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WASF1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol WASF1
Entrez Gene ID 8936
Full Name WAS protein family member 1
Synonyms SCAR1,WAVE,WAVE1
General protein information
Preferred Names

WAS protein family member 1

Names

wiskott-Aldrich syndrome protein family member 1
WASP family protein member 1
homology of dictyostelium scar 1
protein WAVE-1
verprolin homology domain-containing protein 1
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

6

6q21
Summary The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

605035


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_005267207.2 XP_005267264.1 wiskott-Aldrich syndrome protein family member 1 isoform X1
XM_017011444.1 XP_016866933.1 wiskott-Aldrich syndrome protein family member 1 isoform X2
XM_011536233.1 XP_011534535.1 wiskott-Aldrich syndrome protein family member 1 isoform X1
NM_003931.2 NP_003922.1 wiskott-Aldrich syndrome protein family member 1
XM_011536234.1 XP_011534536.1 wiskott-Aldrich syndrome protein family member 1 isoform X1
XM_017011442.1 XP_016866931.1 wiskott-Aldrich syndrome protein family member 1 isoform X2
XM_017011443.1 XP_016866932.1 wiskott-Aldrich syndrome protein family member 1 isoform X2
NM_001024936.1 NP_001020107.1 wiskott-Aldrich syndrome protein family member 1
NM_001024934.1 NP_001020105.1 wiskott-Aldrich syndrome protein family member 1
NM_001024935.1 NP_001020106.1 wiskott-Aldrich syndrome protein family member 1
XM_005267203.3 XP_005267260.1 wiskott-Aldrich syndrome protein family member 1 isoform X1
XM_005267206.2 XP_005267263.1 wiskott-Aldrich syndrome protein family member 1 isoform X1
XM_017011437.1 XP_016866926.1 wiskott-Aldrich syndrome protein family member 1 isoform X1
XM_017011438.1 XP_016866927.1 wiskott-Aldrich syndrome protein family member 1 isoform X1
XM_011536235.1 XP_011534537.1 wiskott-Aldrich syndrome protein family member 1 isoform X2
XM_017011439.1 XP_016866928.1 wiskott-Aldrich syndrome protein family member 1 isoform X2
XM_017011440.1 XP_016866929.1 wiskott-Aldrich syndrome protein family member 1 isoform X2
XM_017011441.1 XP_016866930.1 wiskott-Aldrich syndrome protein family member 1 isoform X2
XM_005267205.2 XP_005267262.1 wiskott-Aldrich syndrome protein family member 1 isoform X1

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) WASF1 XP_005627764.1
Mus musculus (house mouse) Wasf1 NP_114083.1
Rattus norvegicus (Norway rat) Wasf1 NP_001020285.1
Pan troglodytes (chimpanzee) WASF1 NP_001233409.1
Xenopus tropicalis (tropical clawed frog) wasf1 NP_001096217.1
Gallus gallus (chicken) WASF1 XP_004940365.1
Bos taurus (cattle) WASF1 NP_001029189.2
Danio rerio (zebrafish) si:dkeyp-88f5.5 XP_001335802.5
Homo sapiens (human) WASF1 NP_001020105.1

Gene Ontology


Bibliography

Related articles in PubMed

WASP and SCAR are evolutionarily conserved in actin-filled pseudopod-based motility.
Fritz-Laylin LK, Lord SJ, Mullins RD
The Journal of cell biology216(6)1673-1688(2017 Jun)

Knockdown of WAVE1 enhances apoptosis of leukemia cells by downregulating autophagy.
Zhang Z, Wu B, Chai W, Cao L, Wang Y, Yu Y, Yang L
International journal of oncology48(6)2647-56(2016 Jun)

The Association Between WAVE1 and -3 and the ARP2/3 Complex in PC 3 Cells.
Weeks HP, Sanders AJ, Kynaston HG, Jiang WG
Anticancer research36(3)1135-42(2016 Mar)

APP intracellular domain-WAVE1 pathway reduces amyloid-? production.
Ceglia I, Reitz C, Gresack J, Ahn JH, Bustos V, Bleck M, Zhang X, Martin G, Simon SM, Nairn AC, Greengard P, Kim Y
Nature medicine21(9)1054-9(2015 Sep)

Loss of Wave1 gene defines a subtype of lethal prostate cancer.
Sowalsky AG, Sager R, Schaefer RJ, Bratslavsky G, Pandolfi PP, Balk SP, Kotula L
Oncotarget6(14)12383-91(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

WASP and SCAR drive pseudopod formation and are conserved in actin-filled pseudopod-based motility.
Title: WASP and SCAR are evolutionarily conserved in actin-filled pseudopod-based motility.

Results suggested that WAVE1 is a critical pro-autophagic protein capable of enhancing cell survival and regulating chemoresistance in leukemia cells potentially through the Beclin1/Bcl-2 and Beclin1/PI3K- complex-dependent pathways.
Title: Knockdown of WAVE1 enhances apoptosis of leukemia cells by downregulating autophagy.

Results implicate a contributory role of WAVE1 and -3 to the metastatic phenotype of PC-3 cells through their interaction with the ARP2/3 complex.
Title: The Association Between WAVE1 and -3 and the ARP2/3 Complex in PC 3 Cells.

A decrease in amounts of WASF1 mRNA was also observed in human Alzheimer's disease brains, suggesting clinical relevance of the negative feedback circuit involved in homeostatic regulation of Abeta production
Title: APP intracellular domain-WAVE1 pathway reduces amyloid-? production.

we propose that WASF1 status defines a subtype of androgen deprivation therapy -resistant prostate cancer patients
Title: Loss of Wave1 gene defines a subtype of lethal prostate cancer.

The following WASF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WASF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu03620 NM_001024935.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu03620 NM_001024936.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu03620 NM_001024934.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu03620 NM_003931.2
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu03620 XM_011536233.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu03620 XM_005267206.2
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu03620 XM_017011437.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu03620 XM_011536234.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu03620 XM_017011438.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu03620 XM_005267207.2
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu03620 XM_005267203.3
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu03620 XM_005267205.2
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu41010 XM_017011440.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu41010 XM_017011442.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu41010 XM_017011441.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu41010 XM_011536235.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu41010 XM_017011439.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu41010 XM_017011444.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu41010 XM_017011443.1
Latest version!		 Homo sapiens WAS protein family member 1 (WASF1), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu03620
Clone ID Related Accession (Same CDS sequence) XM_005267207.2 , XM_011536233.1 , NM_003931.2 , XM_011536234.1 , NM_001024936.1 , NM_001024934.1 , NM_001024935.1 , XM_005267203.3 , XM_005267206.2 , XM_017011437.1 , XM_017011438.1 , XM_005267205.2
Accession Version NM_003931.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-18
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG719023.1, BI461486.1, D87459.1 and AI928895.1. On Jun 23, 2005 this sequence version replaced NM_003931.1. Transcript Variant: This variant (1) represents the longest transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.166743.1, SRR1660803.26161.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003922.1
CDS838..2517
Misc Feature(1)811..813
Misc Feature(2)1858..1860
Misc Feature(3)<2020..>2304
Misc Feature(4)2302..2304
Misc Feature(5)2317..2400
Exon (1)1..566
Exon (2)567..711
Exon (3)712..809
Exon (4)810..970
Exon (5)971..1105
Exon (6)1106..1259
Exon (7)1260..1377
Exon (8)1378..1550
Exon (9)1551..1730
Exon (10)1731..2359
Exon (11)2360..3220
Translation

Target ORF information:

RefSeq Version NM_003931.2
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_003931.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03620
Clone ID Related Accession (Same CDS sequence) XM_005267207.2 , XM_011536233.1 , NM_003931.2 , XM_011536234.1 , NM_001024936.1 , NM_001024934.1 , NM_001024935.1 , XM_005267203.3 , XM_005267206.2 , XM_017011437.1 , XM_017011438.1 , XM_005267205.2
Accession Version NM_001024936.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-18
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG719023.1, BC068546.1, BC044591.1 and AI928895.1. Transcript Variant: This variant (4) lacks two exons in the 5' UTR, as compared to variant 1. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.165262.1, SRR1660803.30375.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001020107.1
CDS595..2274
Misc Feature(1)568..570
Misc Feature(2)1615..1617
Misc Feature(3)<1777..>2061
Misc Feature(4)2059..2061
Misc Feature(5)2074..2157
Exon (1)1..566
Exon (2)567..727
Exon (3)728..862
Exon (4)863..1016
Exon (5)1017..1134
Exon (6)1135..1307
Exon (7)1308..1487
Exon (8)1488..2116
Exon (9)2117..2977
Translation

Target ORF information:

RefSeq Version NM_001024936.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001024936.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03620
Clone ID Related Accession (Same CDS sequence) XM_005267207.2 , XM_011536233.1 , NM_003931.2 , XM_011536234.1 , NM_001024936.1 , NM_001024934.1 , NM_001024935.1 , XM_005267203.3 , XM_005267206.2 , XM_017011437.1 , XM_017011438.1 , XM_005267205.2
Accession Version NM_001024934.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-18
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG719023.1, BI461486.1, BC068546.1, BC044591.1 and AI928895.1. Transcript Variant: This variant (2) lacks an exon in the 5' UTR, as compared to variant 1. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001020105.1
CDS740..2419
Misc Feature(1)713..715
Misc Feature(2)1760..1762
Misc Feature(3)<1922..>2206
Misc Feature(4)2204..2206
Misc Feature(5)2219..2302
Exon (1)1..566
Exon (2)567..711
Exon (3)712..872
Exon (4)873..1007
Exon (5)1008..1161
Exon (6)1162..1279
Exon (7)1280..1452
Exon (8)1453..1632
Exon (9)1633..2261
Exon (10)2262..3122
Translation

Target ORF information:

RefSeq Version NM_001024934.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001024934.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03620
Clone ID Related Accession (Same CDS sequence) XM_005267207.2 , XM_011536233.1 , NM_003931.2 , XM_011536234.1 , NM_001024936.1 , NM_001024934.1 , NM_001024935.1 , XM_005267203.3 , XM_005267206.2 , XM_017011437.1 , XM_017011438.1 , XM_005267205.2
Accession Version NM_001024935.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-18
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG719023.1, BC044591.1 and AI928895.1. Transcript Variant: This variant (3) lacks an exon in the 5' UTR, as compared to variant 1. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC044591.1, SRR1660805.116055.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001020106.1
CDS693..2372
Misc Feature(1)666..668
Misc Feature(2)1713..1715
Misc Feature(3)<1875..>2159
Misc Feature(4)2157..2159
Misc Feature(5)2172..2255
Exon (1)1..566
Exon (2)567..664
Exon (3)665..825
Exon (4)826..960
Exon (5)961..1114
Exon (6)1115..1232
Exon (7)1233..1405
Exon (8)1406..1585
Exon (9)1586..2214
Exon (10)2215..3075
Translation

Target ORF information:

RefSeq Version NM_001024935.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001024935.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03620
Clone ID Related Accession (Same CDS sequence) XM_005267207.2 , XM_011536233.1 , NM_003931.2 , XM_011536234.1 , NM_001024936.1 , NM_001024934.1 , NM_001024935.1 , XM_005267203.3 , XM_005267206.2 , XM_017011437.1 , XM_017011438.1 , XM_005267205.2
Accession Version XM_005267207.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005267207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005267264.1
CDS165..1844
Misc Feature(1)<1347..>1631
Misc Feature(2)1644..1727
Translation

Target ORF information:

RefSeq Version XM_005267207.2
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005267207.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03620
Clone ID Related Accession (Same CDS sequence) XM_005267207.2 , XM_011536233.1 , NM_003931.2 , XM_011536234.1 , NM_001024936.1 , NM_001024934.1 , NM_001024935.1 , XM_005267203.3 , XM_005267206.2 , XM_017011437.1 , XM_017011438.1 , XM_005267205.2
Accession Version XM_011536233.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534535.1
CDS450..2129
Misc Feature(1)<1632..>1916
Misc Feature(2)1929..2012
Translation

Target ORF information:

RefSeq Version XM_011536233.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011536233.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03620
Clone ID Related Accession (Same CDS sequence) XM_005267207.2 , XM_011536233.1 , NM_003931.2 , XM_011536234.1 , NM_001024936.1 , NM_001024934.1 , NM_001024935.1 , XM_005267203.3 , XM_005267206.2 , XM_017011437.1 , XM_017011438.1 , XM_005267205.2
Accession Version XM_011536234.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534536.1
CDS738..2417
Misc Feature(1)<1920..>2204
Misc Feature(2)2217..2300
Translation

Target ORF information:

RefSeq Version XM_011536234.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011536234.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03620
Clone ID Related Accession (Same CDS sequence) XM_005267207.2 , XM_011536233.1 , NM_003931.2 , XM_011536234.1 , NM_001024936.1 , NM_001024934.1 , NM_001024935.1 , XM_005267203.3 , XM_005267206.2 , XM_017011437.1 , XM_017011438.1 , XM_005267205.2
Accession Version XM_005267203.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005267203.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005267260.1
CDS836..2515
Misc Feature(1)<2018..>2302
Misc Feature(2)2315..2398
Translation

Target ORF information:

RefSeq Version XM_005267203.3
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005267203.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03620
Clone ID Related Accession (Same CDS sequence) XM_005267207.2 , XM_011536233.1 , NM_003931.2 , XM_011536234.1 , NM_001024936.1 , NM_001024934.1 , NM_001024935.1 , XM_005267203.3 , XM_005267206.2 , XM_017011437.1 , XM_017011438.1 , XM_005267205.2
Accession Version XM_005267206.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005267206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005267263.1
CDS350..2029
Misc Feature(1)<1532..>1816
Misc Feature(2)1829..1912
Translation

Target ORF information:

RefSeq Version XM_005267206.2
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005267206.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03620
Clone ID Related Accession (Same CDS sequence) XM_005267207.2 , XM_011536233.1 , NM_003931.2 , XM_011536234.1 , NM_001024936.1 , NM_001024934.1 , NM_001024935.1 , XM_005267203.3 , XM_005267206.2 , XM_017011437.1 , XM_017011438.1 , XM_005267205.2
Accession Version XM_017011437.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016866926.1
CDS858..2537
Misc Feature(1)<2040..>2324
Misc Feature(2)2337..2420
Translation

Target ORF information:

RefSeq Version XM_017011437.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017011437.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03620
Clone ID Related Accession (Same CDS sequence) XM_005267207.2 , XM_011536233.1 , NM_003931.2 , XM_011536234.1 , NM_001024936.1 , NM_001024934.1 , NM_001024935.1 , XM_005267203.3 , XM_005267206.2 , XM_017011437.1 , XM_017011438.1 , XM_005267205.2
Accession Version XM_017011438.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016866927.1
CDS800..2479
Misc Feature(1)<1982..>2266
Misc Feature(2)2279..2362
Translation

Target ORF information:

RefSeq Version XM_017011438.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017011438.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03620
Clone ID Related Accession (Same CDS sequence) XM_005267207.2 , XM_011536233.1 , NM_003931.2 , XM_011536234.1 , NM_001024936.1 , NM_001024934.1 , NM_001024935.1 , XM_005267203.3 , XM_005267206.2 , XM_017011437.1 , XM_017011438.1 , XM_005267205.2
Accession Version XM_005267205.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005267205.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005267262.1
CDS302..1981
Misc Feature(1)<1484..>1768
Misc Feature(2)1781..1864
Translation

Target ORF information:

RefSeq Version XM_005267205.2
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005267205.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
CAGAAAAATC TAGATCGTCC TCATGAACCA GAAAAAGTGC CAAGAGCACC TCATGACAGG 
CGGCGAGAAT GGCAGAAGCT GGCCCAAGGT CCAGAGCTGG CTGAAGATGA TGCTAATCTC 
TTACATAAGC ATATTGAAGT TGCTAATGGC CCAGCCTCTC ATTTTGAAAC AAGACCTCAG 
ACATACGTGG ATCATATGGA TGGATCTTAC TCACTTTCTG CCTTGCCATT TAGTCAGATG 
AGTGAGCTTC TGACTAGAGC TGAGGAAAGG GTATTAGTCA GACCACATGA ACCACCTCCA 
CCTCCACCAA TGCATGGAGC AGGAGATGCA AAACCGATAC CCACCTGTAT CAGTTCTGCT 
ACAGGTTTGA TAGAAAATCG CCCTCAGTCA CCAGCTACAG GCAGAACACC TGTGTTTGTG 
AGCCCCACTC CCCCACCTCC TCCACCACCT CTTCCATCTG CCTTGTCAAC TTCCTCATTA 
AGAGCTTCAA TGACTTCAAC TCCTCCCCCT CCAGTACCTC CCCCACCTCC ACCTCCAGCC 
ACTGCTTTGC AAGCTCCAGC AGTACCACCA CCTCCAGCTC CTCTTCAGAT TGCCCCTGGA 
GTTCTTCACC CAGCTCCTCC TCCAATTGCA CCTCCTCTAG TACAGCCCTC TCCACCAGTA 
GCTAGAGCTG CCCCAGTATG TGAGACTGTA CCAGTTCATC CACTCCCACA AGGTGAAGTT 
CAGGGGCTGC CTCCACCCCC ACCACCGCCT CCTCTGCCTC CACCTGGCAT TCGACCATCA 
TCACCTGTCA CAGTTACAGC TCTTGCTCAT CCTCCCTCTG GGCTACATCC AACTCCATCT 
ACTGCCCCAG GTCCCCATGT TCCATTAATG CCTCCATCTC CTCCATCACA AGTTATACCT 
GCTTCTGAGC CAAAGCGCCA TCCATCAACC CTACCTGTAA TCAGTGATGC CAGGAGTGTG 
CTACTGGAAG CAATACGAAA AGGTATTCAG CTACGCAAAG TAGAAGAGCA GCGTGAACAG 
GAAGCTAAGC ATGAACGCAT TGAAAACGAT GTTGCCACCA TCCTGTCTCG CCGTATTGCT 
GTTGAATATA GTGATTCGGA AGATGATTCA GAATTTGATG AAGTAGATTG GTTGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41010
Clone ID Related Accession (Same CDS sequence) XM_017011444.1 , XM_017011442.1 , XM_017011443.1 , XM_011536235.1 , XM_017011439.1 , XM_017011440.1 , XM_017011441.1
Accession Version XM_017011444.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016866933.1
CDS350..2026
Misc Feature(1)1826..1909
Translation

Target ORF information:

RefSeq Version XM_017011444.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017011444.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41010
Clone ID Related Accession (Same CDS sequence) XM_017011444.1 , XM_017011442.1 , XM_017011443.1 , XM_011536235.1 , XM_017011439.1 , XM_017011440.1 , XM_017011441.1
Accession Version XM_017011442.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016866931.1
CDS596..2272
Misc Feature(1)2072..2155
Translation

Target ORF information:

RefSeq Version XM_017011442.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017011442.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41010
Clone ID Related Accession (Same CDS sequence) XM_017011444.1 , XM_017011442.1 , XM_017011443.1 , XM_011536235.1 , XM_017011439.1 , XM_017011440.1 , XM_017011441.1
Accession Version XM_017011443.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016866932.1
CDS198..1874
Misc Feature(1)1674..1757
Translation

Target ORF information:

RefSeq Version XM_017011443.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017011443.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41010
Clone ID Related Accession (Same CDS sequence) XM_017011444.1 , XM_017011442.1 , XM_017011443.1 , XM_011536235.1 , XM_017011439.1 , XM_017011440.1 , XM_017011441.1
Accession Version XM_011536235.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534537.1
CDS796..2472
Misc Feature(1)2272..2355
Translation

Target ORF information:

RefSeq Version XM_011536235.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011536235.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41010
Clone ID Related Accession (Same CDS sequence) XM_017011444.1 , XM_017011442.1 , XM_017011443.1 , XM_011536235.1 , XM_017011439.1 , XM_017011440.1 , XM_017011441.1
Accession Version XM_017011439.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016866928.1
CDS391..2067
Misc Feature(1)1867..1950
Translation

Target ORF information:

RefSeq Version XM_017011439.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017011439.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41010
Clone ID Related Accession (Same CDS sequence) XM_017011444.1 , XM_017011442.1 , XM_017011443.1 , XM_011536235.1 , XM_017011439.1 , XM_017011440.1 , XM_017011441.1
Accession Version XM_017011440.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016866929.1
CDS268..1944
Misc Feature(1)1744..1827
Translation

Target ORF information:

RefSeq Version XM_017011440.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017011440.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41010
Clone ID Related Accession (Same CDS sequence) XM_017011444.1 , XM_017011442.1 , XM_017011443.1 , XM_011536235.1 , XM_017011439.1 , XM_017011440.1 , XM_017011441.1
Accession Version XM_017011441.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product wiskott-Aldrich syndrome protein family member 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016866930.1
CDS302..1978
Misc Feature(1)1778..1861
Translation

Target ORF information:

RefSeq Version XM_017011441.1
Organism Homo sapiens(human)
Definition Homo sapiens WAS protein family member 1 (WASF1), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017011441.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGCTAG TGAAAAGAAA CATCGATCCT AGGCACTTGT GCCACACAGC ACTGCCTAGA 
GGCATTAAGA ATGAACTGGA ATGTGTAACC AATATTTCCT TGGCAAATAT AATTAGACAA 
CTAAGTAGCC TAAGTAAATA TGCTGAAGAT ATATTTGGAG AATTATTCAA TGAAGCACAT 
AGTTTTTCCT TCAGAGTCAA CTCATTGCAA GAACGTGTGG ACCGTTTATC TGTTAGTGTT 
ACACAGCTTG ATCCAAAGGA AGAAGAATTG TCTTTGCAAG ATATAACAAT GAGGAAAGCT 
TTCCGAAGTT CTACAATTCA AGACCAGCAG CTTTTCGATC GCAAGACTTT GCCTATTCCA 
TTACAGGAGA CGTACGATGT TTGTGAACAG CCTCCACCTC TCAATATACT CACTCCTTAT 
AGAGATGATG GTAAAGAAGG TCTGAAGTTT TATACCAATC CTTCGTATTT CTTTGATCTA 
TGGAAAGAAA AAATGTTGCA AGATACAGAG GATAAGAGGA AGGAAAAGAG GAAGCAGAAG 
AAAAATCTAG ATCGTCCTCA TGAACCAGAA AAAGTGCCAA GAGCACCTCA TGACAGGCGG 
CGAGAATGGC AGAAGCTGGC CCAAGGTCCA GAGCTGGCTG AAGATGATGC TAATCTCTTA 
CATAAGCATA TTGAAGTTGC TAATGGCCCA GCCTCTCATT TTGAAACAAG ACCTCAGACA 
TACGTGGATC ATATGGATGG ATCTTACTCA CTTTCTGCCT TGCCATTTAG TCAGATGAGT 
GAGCTTCTGA CTAGAGCTGA GGAAAGGGTA TTAGTCAGAC CACATGAACC ACCTCCACCT 
CCACCAATGC ATGGAGCAGG AGATGCAAAA CCGATACCCA CCTGTATCAG TTCTGCTACA 
GGTTTGATAG AAAATCGCCC TCAGTCACCA GCTACAGGCA GAACACCTGT GTTTGTGAGC 
CCCACTCCCC CACCTCCTCC ACCACCTCTT CCATCTGCCT TGTCAACTTC CTCATTAAGA 
GCTTCAATGA CTTCAACTCC TCCCCCTCCA GTACCTCCCC CACCTCCACC TCCAGCCACT 
GCTTTGCAAG CTCCAGCAGT ACCACCACCT CCAGCTCCTC TTCAGATTGC CCCTGGAGTT 
CTTCACCCAG CTCCTCCTCC AATTGCACCT CCTCTAGTAC AGCCCTCTCC ACCAGTAGCT 
AGAGCTGCCC CAGTATGTGA GACTGTACCA GTTCATCCAC TCCCACAAGG TGAAGTTCAG 
GGGCTGCCTC CACCCCCACC ACCGCCTCCT CTGCCTCCAC CTGGCATTCG ACCATCATCA 
CCTGTCACAG TTACAGCTCT TGCTCATCCT CCCTCTGGGC TACATCCAAC TCCATCTACT 
GCCCCAGGTC CCCATGTTCC ATTAATGCCT CCATCTCCTC CATCACAAGT TATACCTGCT 
TCTGAGCCAA AGCGCCATCC ATCAACCCTA CCTGTAATCA GTGATGCCAG GAGTGTGCTA 
CTGGAAGCAA TACGAAAAGG TATTCAGCTA CGCAAAGTAG AAGAGCAGCG TGAACAGGAA 
GCTAAGCATG AACGCATTGA AAACGATGTT GCCACCATCC TGTCTCGCCG TATTGCTGTT 
GAATATAGTG ATTCGGAAGA TGATTCAGAA TTTGATGAAG TAGATTGGTT GGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

WASP and SCAR are evolutionarily conserved in actin-filled pseudopod-based motility.
The Journal of cell biology216(6)1673-1688(2017 Jun)
Fritz-Laylin LK,Lord SJ,Mullins RD


book

Knockdown of WAVE1 enhances apoptosis of leukemia cells by downregulating autophagy.
International journal of oncology48(6)2647-56(2016 Jun)
Zhang Z,Wu B,Chai W,Cao L,Wang Y,Yu Y,Yang L


book

The Association Between WAVE1 and -3 and the ARP2/3 Complex in PC 3 Cells.
Anticancer research36(3)1135-42(2016 Mar)
Weeks HP,Sanders AJ,Kynaston HG,Jiang WG


book

APP intracellular domain-WAVE1 pathway reduces amyloid-? production.
Nature medicine21(9)1054-9(2015 Sep)
Ceglia I,Reitz C,Gresack J,Ahn JH,Bustos V,Bleck M,Zhang X,Martin G,Simon SM,Nairn AC,Greengard P,Kim Y


book

Loss of Wave1 gene defines a subtype of lethal prostate cancer.
Oncotarget6(14)12383-91(2015 May)
Sowalsky AG,Sager R,Schaefer RJ,Bratslavsky G,Pandolfi PP,Balk SP,Kotula L