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CHST3 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol CHST3
Entrez Gene ID 9469
Full Name carbohydrate sulfotransferase 3
Synonyms C6ST,C6ST1,HSD
General protein information
Preferred Names

carbohydrate sulfotransferase 3

Names

carbohydrate sulfotransferase 3
C6ST-1
GST-0
carbohydrate (chondroitin 6) sulfotransferase 3
chondroitin 6 sulfotransferase 1
chondroitin 6-O-sulfotransferase 1
galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

10

10q22.1

Summary This gene encodes an enzyme which catalyzes the sulfation of chondroitin, a proteoglycan found in the extracellular matrix and most cells which is involved in cell migration and differentiation. Mutations in this gene are associated with spondylepiphyseal dysplasia and humerospinal dysostosis. [provided by RefSeq, Mar 2009].
Disorder MIM:

603799

Disorder Html: Spondyloepiphyseal dysplasia with congenital joint dislocations,

mRNA and Protein(s)

mRNA Protein Name
XM_011540369.2 XP_011538671.1 carbohydrate sulfotransferase 3 isoform X1
NM_004273.4 NP_004264.2 carbohydrate sulfotransferase 3
XM_006718075.3 XP_006718138.1 carbohydrate sulfotransferase 3 isoform X1



Homo sapiens (human) CHST3 NP_004264.2
Pan troglodytes (chimpanzee) CHST3 XP_521690.1
Macaca mulatta (rhesus monkey) CHST3 XP_001106464.2
Mus musculus (house mouse) Chst3 NP_058083.2
Rattus norvegicus (Norway rat) Chst3 NP_445860.1
Gallus gallus (chicken) CHST3 NP_990452.1
Danio rerio (zebrafish) chst3a NP_001139043.1
Canis lupus familiaris (dog) CHST3 XP_003639041.1
Bos taurus (cattle) CHST3 NP_001192524.1
Danio rerio (zebrafish) chst3b NP_001103846.1
Xenopus tropicalis (tropical clawed frog) chst3 XP_002939563.2


Related articles in PubMed

Craniosynostosis: a previously unreported association with CHST3-related skeletal dysplasia (autosomal recessive Larsen syndrome).
Searle C, Jewell R, Kraft J, Stoebe P, Chumas P, Titheradge H, Kraus A, Gencik M, Hobson E
Clinical dysmorphology23(1)12-5(2014 Jan)

Lumbar disc degeneration is linked to a carbohydrate sulfotransferase 3 variant.
Song YQ, Karasugi T, Cheung KM, Chiba K, Ho DW, Miyake A, Kao PY, Sze KL, Yee A, Takahashi A, Kawaguchi Y, Mikami Y, Matsumoto M, Togawa D, Kanayama M, Shi D, Dai J, Jiang Q, Wu C, Tian W, Wang N, Leong JC, Luk KD, Yip SP, Cherny SS, Wang J, Mundlos S, Kelempisioti A, Eskola PJ, Männikkö M, Mäkelä P, Karppinen J, Järvelin MR, O'Reilly PF, Kubo M, Kimura T, Kubo T, Toyama Y, Mizuta H, Cheah KS, Tsunoda T, Sham PC, Ikegawa S, Chan D
The Journal of clinical investigation123(11)4909-17(2013 Nov)

Persistent cortical plasticity by upregulation of chondroitin 6-sulfation.
Miyata S, Komatsu Y, Yoshimura Y, Taya C, Kitagawa H
Nature neuroscience15(3)414-22, S1-2(2012 Jan)

Image-based genome-wide siRNA screen identifies selective autophagy factors.
Orvedahl A, Sumpter R Jr, Xiao G, Ng A, Zou Z, Tang Y, Narimatsu M, Gilpin C, Sun Q, Roth M, Forst CV, Wrana JL, Zhang YE, Luby-Phelps K, Xavier RJ, Xie Y, Levine B
Nature480(7375)113-7(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel CHST3 allele associated with spondyloepiphyseal dysplasia and hearing loss has been described in a consanguineous Pakistani pedigree.
Title: A novel CHST3 allele associated with spondyloepiphyseal dysplasia and hearing loss in Pakistani kindred.

Sequencing of CHST3 detected a previously unreported homozygous duplication c.407_426dup (p.Thr143Cysfs*80). The mutation is predicted to lead to frameshift and introduction of a premature stopcodon.
Title: Craniosynostosis: a previously unreported association with CHST3-related skeletal dysplasia (autosomal recessive Larsen syndrome).

expression of CHST3 mRNA was significantly reduced in the intervertebral disc cells of subjects carrying the A allele of rs4148941
Title: Lumbar disc degeneration is linked to a carbohydrate sulfotransferase 3 variant.

The results of this study indicated that the critical period for cortical plasticity is regulated by the 4S/6S ratio of CSPGs, which determines the maturation of parvalbumin-expressing interneurons.
Title: Persistent cortical plasticity by upregulation of chondroitin 6-sulfation.

Vertebral changes, normal carpal age, lack of facial flattening, and recessive inheritance are characteristic and distinguishes CHST3 deficiency from other disorders with congenital dislocations.
Title: Phenotypic features of carbohydrate sulfotransferase 3 (CHST3) deficiency in 24 patients: congenital dislocations and vertebral changes as principal diagnostic features.

The following CHST3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHST3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu23151 NM_004273.4
Latest version!
Homo sapiens carbohydrate sulfotransferase 3 (CHST3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu23151 XM_006718075.3
Latest version!
Homo sapiens carbohydrate sulfotransferase 3 (CHST3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu23151 XM_011540369.2
Latest version!
Homo sapiens carbohydrate sulfotransferase 3 (CHST3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23151
Clone ID Related Accession (Same CDS sequence) XM_011540369.2 , NM_004273.4 , XM_006718075.3
Accession Version NM_004273.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1440bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-17
Organism Homo sapiens(Human)
Product carbohydrate sulfotransferase 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB017915.1, AC022392.5 and AI041015.1. This sequence is a reference standard in the RefSeqGene project. On Mar 4, 2009 this sequence version replaced NM_004273.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB017915.1, AB012192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAGAAAG GACTCACTTT GCCCCAGGAC TGCCGGGACT TTGTGCACAG CCTGAAGATG 
AGAAGCAAAT ACGCCCTTTT CTTGGTTTTT GTGGTGATAG TTTTTGTCTT CATCGAAAAG
GAAAATAAAA TCATATCAAG GGTCTCAGAC AAGCTGAAGC AGATTCCCCA AGCTCTAGCA
GATGCCAACA GCACCGACCC AGCCCTGATC TTAGCTGAGA ACGCATCTCT CTTGTCCCTG
AGCGAGCTCG ATTCAGCCTT CTCCCAGCTT CAGAGCCGTC TCCGCAACCT CAGCTTGCAG
CTGGGCGTGG AGCCAGCCAT GGAGGCCGCA GGGGAGGAAG AGGAAGAGCA GAGAAAGGAG
GAGGAGCCGC CCAGACCGGC CGTGGCGGGG CCCCGGCGCC ACGTGCTGCT CATGGCCACC
ACGCGCACCG GCTCCTCGTT CGTGGGCGAG TTCTTCAACC AGCAGGGCAA CATCTTCTAC
CTCTTCGAGC CGCTGTGGCA CATCGAGCGC ACAGTGTCCT TCGAGCCGGG GGGCGCCAAC
GCCGCGGGCT CGGCCCTGGT GTACCGCGAC GTGCTCAAGC AGCTCTTCCT GTGCGACCTG
TACGTGCTGG AGCACTTCAT CACGCCGCTG CCCGAGGACC ACCTGACTCA GTTCATGTTC
CGCCGGGGCT CCAGCCGCTC CCTGTGCGAG GACCCCGTCT GTACGCCCTT CGTCAAGAAG
GTCTTCGAGA AGTACCACTG CAAGAACCGC CGCTGCGGCC CCCTCAACGT GACGCTGGCC
GCAGAGGCCT GCCGCCGCAA GGAGCACATG GCCCTCAAGG CGGTGCGCAT CCGGCAGCTG
GAGTTCCTGC AGCCGCTGGC CGAGGACCCC CGCCTGGACC TGCGCGTCAT CCAGCTGGTG
CGCGACCCCC GGGCCGTGCT GGCCTCGCGC ATGGTGGCCT TCGCCGGCAA GTATAAGACC
TGGAAGAAGT GGCTGGACGA CGAGGGCCAG GACGGCCTGA GGGAAGAGGA GGTGCAGCGG
CTGCGGGGCA ACTGCGAGAG CATCCGCCTG TCCGCGGAGC TGGGGCTGCG GCAGCCCGCC
TGGCTGCGGG GCCGCTACAT GCTGGTGCGC TACGAGGACG TGGCACGCGG GCCGCTGCAG
AAGGCCCGCG AGATGTACCG CTTCGCCGGC ATCCCCCTGA CCCCGCAGGT GGAAGACTGG
ATCCAAAAGA ACACGCAGGC GGCCCACGAC GGCAGCGGCA TCTACTCCAC GCAGAAGAAC
TCCTCGGAGC AGTTCGAGAA GTGGCGCTTC AGCATGCCCT TCAAGCTGGC CCAGGTGGTG
CAGGCCGCCT GCGGCCCTGC CATGCGCCTC TTCGGCTACA AACTGGCGCG GGACGCCGCC
GCCCTCACCA ACCGCTCAGT CAGCCTGCTG GAGGAGAGGG GCACCTTCTG GGTCACGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004264.2
CDS441..1880
Misc Feature(1)378..380
Misc Feature(2)501..554
Misc Feature(3)<1230..1796
Exon (1)1..333
Exon (2)334..580
Exon (3)581..6973
Translation

Target ORF information:

RefSeq Version NM_004273.4
Organism Homo sapiens(Human)
Definition Homo sapiens carbohydrate sulfotransferase 3 (CHST3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004273.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAGAAAG GACTCACTTT GCCCCAGGAC TGCCGGGACT TTGTGCACAG CCTGAAGATG 
AGAAGCAAAT ACGCCCTTTT CTTGGTTTTT GTGGTGATAG TTTTTGTCTT CATCGAAAAG
GAAAATAAAA TCATATCAAG GGTCTCAGAC AAGCTGAAGC AGATTCCCCA AGCTCTAGCA
GATGCCAACA GCACCGACCC AGCCCTGATC TTAGCTGAGA ACGCATCTCT CTTGTCCCTG
AGCGAGCTCG ATTCAGCCTT CTCCCAGCTT CAGAGCCGTC TCCGCAACCT CAGCTTGCAG
CTGGGCGTGG AGCCAGCCAT GGAGGCCGCA GGGGAGGAAG AGGAAGAGCA GAGAAAGGAG
GAGGAGCCGC CCAGACCGGC CGTGGCGGGG CCCCGGCGCC ACGTGCTGCT CATGGCCACC
ACGCGCACCG GCTCCTCGTT CGTGGGCGAG TTCTTCAACC AGCAGGGCAA CATCTTCTAC
CTCTTCGAGC CGCTGTGGCA CATCGAGCGC ACAGTGTCCT TCGAGCCGGG GGGCGCCAAC
GCCGCGGGCT CGGCCCTGGT GTACCGCGAC GTGCTCAAGC AGCTCTTCCT GTGCGACCTG
TACGTGCTGG AGCACTTCAT CACGCCGCTG CCCGAGGACC ACCTGACTCA GTTCATGTTC
CGCCGGGGCT CCAGCCGCTC CCTGTGCGAG GACCCCGTCT GTACGCCCTT CGTCAAGAAG
GTCTTCGAGA AGTACCACTG CAAGAACCGC CGCTGCGGCC CCCTCAACGT GACGCTGGCC
GCAGAGGCCT GCCGCCGCAA GGAGCACATG GCCCTCAAGG CGGTGCGCAT CCGGCAGCTG
GAGTTCCTGC AGCCGCTGGC CGAGGACCCC CGCCTGGACC TGCGCGTCAT CCAGCTGGTG
CGCGACCCCC GGGCCGTGCT GGCCTCGCGC ATGGTGGCCT TCGCCGGCAA GTATAAGACC
TGGAAGAAGT GGCTGGACGA CGAGGGCCAG GACGGCCTGA GGGAAGAGGA GGTGCAGCGG
CTGCGGGGCA ACTGCGAGAG CATCCGCCTG TCCGCGGAGC TGGGGCTGCG GCAGCCCGCC
TGGCTGCGGG GCCGCTACAT GCTGGTGCGC TACGAGGACG TGGCACGCGG GCCGCTGCAG
AAGGCCCGCG AGATGTACCG CTTCGCCGGC ATCCCCCTGA CCCCGCAGGT GGAAGACTGG
ATCCAAAAGA ACACGCAGGC GGCCCACGAC GGCAGCGGCA TCTACTCCAC GCAGAAGAAC
TCCTCGGAGC AGTTCGAGAA GTGGCGCTTC AGCATGCCCT TCAAGCTGGC CCAGGTGGTG
CAGGCCGCCT GCGGCCCTGC CATGCGCCTC TTCGGCTACA AACTGGCGCG GGACGCCGCC
GCCCTCACCA ACCGCTCAGT CAGCCTGCTG GAGGAGAGGG GCACCTTCTG GGTCACGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23151
Clone ID Related Accession (Same CDS sequence) XM_011540369.2 , NM_004273.4 , XM_006718075.3
Accession Version XM_011540369.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1440bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product carbohydrate sulfotransferase 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAGAAAG GACTCACTTT GCCCCAGGAC TGCCGGGACT TTGTGCACAG CCTGAAGATG 
AGAAGCAAAT ACGCCCTTTT CTTGGTTTTT GTGGTGATAG TTTTTGTCTT CATCGAAAAG
GAAAATAAAA TCATATCAAG GGTCTCAGAC AAGCTGAAGC AGATTCCCCA AGCTCTAGCA
GATGCCAACA GCACCGACCC AGCCCTGATC TTAGCTGAGA ACGCATCTCT CTTGTCCCTG
AGCGAGCTCG ATTCAGCCTT CTCCCAGCTT CAGAGCCGTC TCCGCAACCT CAGCTTGCAG
CTGGGCGTGG AGCCAGCCAT GGAGGCCGCA GGGGAGGAAG AGGAAGAGCA GAGAAAGGAG
GAGGAGCCGC CCAGACCGGC CGTGGCGGGG CCCCGGCGCC ACGTGCTGCT CATGGCCACC
ACGCGCACCG GCTCCTCGTT CGTGGGCGAG TTCTTCAACC AGCAGGGCAA CATCTTCTAC
CTCTTCGAGC CGCTGTGGCA CATCGAGCGC ACAGTGTCCT TCGAGCCGGG GGGCGCCAAC
GCCGCGGGCT CGGCCCTGGT GTACCGCGAC GTGCTCAAGC AGCTCTTCCT GTGCGACCTG
TACGTGCTGG AGCACTTCAT CACGCCGCTG CCCGAGGACC ACCTGACTCA GTTCATGTTC
CGCCGGGGCT CCAGCCGCTC CCTGTGCGAG GACCCCGTCT GTACGCCCTT CGTCAAGAAG
GTCTTCGAGA AGTACCACTG CAAGAACCGC CGCTGCGGCC CCCTCAACGT GACGCTGGCC
GCAGAGGCCT GCCGCCGCAA GGAGCACATG GCCCTCAAGG CGGTGCGCAT CCGGCAGCTG
GAGTTCCTGC AGCCGCTGGC CGAGGACCCC CGCCTGGACC TGCGCGTCAT CCAGCTGGTG
CGCGACCCCC GGGCCGTGCT GGCCTCGCGC ATGGTGGCCT TCGCCGGCAA GTATAAGACC
TGGAAGAAGT GGCTGGACGA CGAGGGCCAG GACGGCCTGA GGGAAGAGGA GGTGCAGCGG
CTGCGGGGCA ACTGCGAGAG CATCCGCCTG TCCGCGGAGC TGGGGCTGCG GCAGCCCGCC
TGGCTGCGGG GCCGCTACAT GCTGGTGCGC TACGAGGACG TGGCACGCGG GCCGCTGCAG
AAGGCCCGCG AGATGTACCG CTTCGCCGGC ATCCCCCTGA CCCCGCAGGT GGAAGACTGG
ATCCAAAAGA ACACGCAGGC GGCCCACGAC GGCAGCGGCA TCTACTCCAC GCAGAAGAAC
TCCTCGGAGC AGTTCGAGAA GTGGCGCTTC AGCATGCCCT TCAAGCTGGC CCAGGTGGTG
CAGGCCGCCT GCGGCCCTGC CATGCGCCTC TTCGGCTACA AACTGGCGCG GGACGCCGCC
GCCCTCACCA ACCGCTCAGT CAGCCTGCTG GAGGAGAGGG GCACCTTCTG GGTCACGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538671.1
CDS2367..3806
Misc Feature(1)<3156..3722
Translation

Target ORF information:

RefSeq Version XM_011540369.2
Organism Homo sapiens(Human)
Definition Homo sapiens carbohydrate sulfotransferase 3 (CHST3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011540369.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAGAAAG GACTCACTTT GCCCCAGGAC TGCCGGGACT TTGTGCACAG CCTGAAGATG 
AGAAGCAAAT ACGCCCTTTT CTTGGTTTTT GTGGTGATAG TTTTTGTCTT CATCGAAAAG
GAAAATAAAA TCATATCAAG GGTCTCAGAC AAGCTGAAGC AGATTCCCCA AGCTCTAGCA
GATGCCAACA GCACCGACCC AGCCCTGATC TTAGCTGAGA ACGCATCTCT CTTGTCCCTG
AGCGAGCTCG ATTCAGCCTT CTCCCAGCTT CAGAGCCGTC TCCGCAACCT CAGCTTGCAG
CTGGGCGTGG AGCCAGCCAT GGAGGCCGCA GGGGAGGAAG AGGAAGAGCA GAGAAAGGAG
GAGGAGCCGC CCAGACCGGC CGTGGCGGGG CCCCGGCGCC ACGTGCTGCT CATGGCCACC
ACGCGCACCG GCTCCTCGTT CGTGGGCGAG TTCTTCAACC AGCAGGGCAA CATCTTCTAC
CTCTTCGAGC CGCTGTGGCA CATCGAGCGC ACAGTGTCCT TCGAGCCGGG GGGCGCCAAC
GCCGCGGGCT CGGCCCTGGT GTACCGCGAC GTGCTCAAGC AGCTCTTCCT GTGCGACCTG
TACGTGCTGG AGCACTTCAT CACGCCGCTG CCCGAGGACC ACCTGACTCA GTTCATGTTC
CGCCGGGGCT CCAGCCGCTC CCTGTGCGAG GACCCCGTCT GTACGCCCTT CGTCAAGAAG
GTCTTCGAGA AGTACCACTG CAAGAACCGC CGCTGCGGCC CCCTCAACGT GACGCTGGCC
GCAGAGGCCT GCCGCCGCAA GGAGCACATG GCCCTCAAGG CGGTGCGCAT CCGGCAGCTG
GAGTTCCTGC AGCCGCTGGC CGAGGACCCC CGCCTGGACC TGCGCGTCAT CCAGCTGGTG
CGCGACCCCC GGGCCGTGCT GGCCTCGCGC ATGGTGGCCT TCGCCGGCAA GTATAAGACC
TGGAAGAAGT GGCTGGACGA CGAGGGCCAG GACGGCCTGA GGGAAGAGGA GGTGCAGCGG
CTGCGGGGCA ACTGCGAGAG CATCCGCCTG TCCGCGGAGC TGGGGCTGCG GCAGCCCGCC
TGGCTGCGGG GCCGCTACAT GCTGGTGCGC TACGAGGACG TGGCACGCGG GCCGCTGCAG
AAGGCCCGCG AGATGTACCG CTTCGCCGGC ATCCCCCTGA CCCCGCAGGT GGAAGACTGG
ATCCAAAAGA ACACGCAGGC GGCCCACGAC GGCAGCGGCA TCTACTCCAC GCAGAAGAAC
TCCTCGGAGC AGTTCGAGAA GTGGCGCTTC AGCATGCCCT TCAAGCTGGC CCAGGTGGTG
CAGGCCGCCT GCGGCCCTGC CATGCGCCTC TTCGGCTACA AACTGGCGCG GGACGCCGCC
GCCCTCACCA ACCGCTCAGT CAGCCTGCTG GAGGAGAGGG GCACCTTCTG GGTCACGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23151
Clone ID Related Accession (Same CDS sequence) XM_011540369.2 , NM_004273.4 , XM_006718075.3
Accession Version XM_006718075.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1440bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product carbohydrate sulfotransferase 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718075.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAGAAAG GACTCACTTT GCCCCAGGAC TGCCGGGACT TTGTGCACAG CCTGAAGATG 
AGAAGCAAAT ACGCCCTTTT CTTGGTTTTT GTGGTGATAG TTTTTGTCTT CATCGAAAAG
GAAAATAAAA TCATATCAAG GGTCTCAGAC AAGCTGAAGC AGATTCCCCA AGCTCTAGCA
GATGCCAACA GCACCGACCC AGCCCTGATC TTAGCTGAGA ACGCATCTCT CTTGTCCCTG
AGCGAGCTCG ATTCAGCCTT CTCCCAGCTT CAGAGCCGTC TCCGCAACCT CAGCTTGCAG
CTGGGCGTGG AGCCAGCCAT GGAGGCCGCA GGGGAGGAAG AGGAAGAGCA GAGAAAGGAG
GAGGAGCCGC CCAGACCGGC CGTGGCGGGG CCCCGGCGCC ACGTGCTGCT CATGGCCACC
ACGCGCACCG GCTCCTCGTT CGTGGGCGAG TTCTTCAACC AGCAGGGCAA CATCTTCTAC
CTCTTCGAGC CGCTGTGGCA CATCGAGCGC ACAGTGTCCT TCGAGCCGGG GGGCGCCAAC
GCCGCGGGCT CGGCCCTGGT GTACCGCGAC GTGCTCAAGC AGCTCTTCCT GTGCGACCTG
TACGTGCTGG AGCACTTCAT CACGCCGCTG CCCGAGGACC ACCTGACTCA GTTCATGTTC
CGCCGGGGCT CCAGCCGCTC CCTGTGCGAG GACCCCGTCT GTACGCCCTT CGTCAAGAAG
GTCTTCGAGA AGTACCACTG CAAGAACCGC CGCTGCGGCC CCCTCAACGT GACGCTGGCC
GCAGAGGCCT GCCGCCGCAA GGAGCACATG GCCCTCAAGG CGGTGCGCAT CCGGCAGCTG
GAGTTCCTGC AGCCGCTGGC CGAGGACCCC CGCCTGGACC TGCGCGTCAT CCAGCTGGTG
CGCGACCCCC GGGCCGTGCT GGCCTCGCGC ATGGTGGCCT TCGCCGGCAA GTATAAGACC
TGGAAGAAGT GGCTGGACGA CGAGGGCCAG GACGGCCTGA GGGAAGAGGA GGTGCAGCGG
CTGCGGGGCA ACTGCGAGAG CATCCGCCTG TCCGCGGAGC TGGGGCTGCG GCAGCCCGCC
TGGCTGCGGG GCCGCTACAT GCTGGTGCGC TACGAGGACG TGGCACGCGG GCCGCTGCAG
AAGGCCCGCG AGATGTACCG CTTCGCCGGC ATCCCCCTGA CCCCGCAGGT GGAAGACTGG
ATCCAAAAGA ACACGCAGGC GGCCCACGAC GGCAGCGGCA TCTACTCCAC GCAGAAGAAC
TCCTCGGAGC AGTTCGAGAA GTGGCGCTTC AGCATGCCCT TCAAGCTGGC CCAGGTGGTG
CAGGCCGCCT GCGGCCCTGC CATGCGCCTC TTCGGCTACA AACTGGCGCG GGACGCCGCC
GCCCTCACCA ACCGCTCAGT CAGCCTGCTG GAGGAGAGGG GCACCTTCTG GGTCACGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006718138.1
CDS674..2113
Misc Feature(1)<1463..2029
Translation

Target ORF information:

RefSeq Version XM_006718075.3
Organism Homo sapiens(Human)
Definition Homo sapiens carbohydrate sulfotransferase 3 (CHST3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006718075.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAGAAAG GACTCACTTT GCCCCAGGAC TGCCGGGACT TTGTGCACAG CCTGAAGATG 
AGAAGCAAAT ACGCCCTTTT CTTGGTTTTT GTGGTGATAG TTTTTGTCTT CATCGAAAAG
GAAAATAAAA TCATATCAAG GGTCTCAGAC AAGCTGAAGC AGATTCCCCA AGCTCTAGCA
GATGCCAACA GCACCGACCC AGCCCTGATC TTAGCTGAGA ACGCATCTCT CTTGTCCCTG
AGCGAGCTCG ATTCAGCCTT CTCCCAGCTT CAGAGCCGTC TCCGCAACCT CAGCTTGCAG
CTGGGCGTGG AGCCAGCCAT GGAGGCCGCA GGGGAGGAAG AGGAAGAGCA GAGAAAGGAG
GAGGAGCCGC CCAGACCGGC CGTGGCGGGG CCCCGGCGCC ACGTGCTGCT CATGGCCACC
ACGCGCACCG GCTCCTCGTT CGTGGGCGAG TTCTTCAACC AGCAGGGCAA CATCTTCTAC
CTCTTCGAGC CGCTGTGGCA CATCGAGCGC ACAGTGTCCT TCGAGCCGGG GGGCGCCAAC
GCCGCGGGCT CGGCCCTGGT GTACCGCGAC GTGCTCAAGC AGCTCTTCCT GTGCGACCTG
TACGTGCTGG AGCACTTCAT CACGCCGCTG CCCGAGGACC ACCTGACTCA GTTCATGTTC
CGCCGGGGCT CCAGCCGCTC CCTGTGCGAG GACCCCGTCT GTACGCCCTT CGTCAAGAAG
GTCTTCGAGA AGTACCACTG CAAGAACCGC CGCTGCGGCC CCCTCAACGT GACGCTGGCC
GCAGAGGCCT GCCGCCGCAA GGAGCACATG GCCCTCAAGG CGGTGCGCAT CCGGCAGCTG
GAGTTCCTGC AGCCGCTGGC CGAGGACCCC CGCCTGGACC TGCGCGTCAT CCAGCTGGTG
CGCGACCCCC GGGCCGTGCT GGCCTCGCGC ATGGTGGCCT TCGCCGGCAA GTATAAGACC
TGGAAGAAGT GGCTGGACGA CGAGGGCCAG GACGGCCTGA GGGAAGAGGA GGTGCAGCGG
CTGCGGGGCA ACTGCGAGAG CATCCGCCTG TCCGCGGAGC TGGGGCTGCG GCAGCCCGCC
TGGCTGCGGG GCCGCTACAT GCTGGTGCGC TACGAGGACG TGGCACGCGG GCCGCTGCAG
AAGGCCCGCG AGATGTACCG CTTCGCCGGC ATCCCCCTGA CCCCGCAGGT GGAAGACTGG
ATCCAAAAGA ACACGCAGGC GGCCCACGAC GGCAGCGGCA TCTACTCCAC GCAGAAGAAC
TCCTCGGAGC AGTTCGAGAA GTGGCGCTTC AGCATGCCCT TCAAGCTGGC CCAGGTGGTG
CAGGCCGCCT GCGGCCCTGC CATGCGCCTC TTCGGCTACA AACTGGCGCG GGACGCCGCC
GCCCTCACCA ACCGCTCAGT CAGCCTGCTG GAGGAGAGGG GCACCTTCTG GGTCACGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Craniosynostosis: a previously unreported association with CHST3-related skeletal dysplasia (autosomal recessive Larsen syndrome).
Clinical dysmorphology23(1)12-5(2014 Jan)
Searle C,Jewell R,Kraft J,Stoebe P,Chumas P,Titheradge H,Kraus A,Gencik M,Hobson E


book

Lumbar disc degeneration is linked to a carbohydrate sulfotransferase 3 variant.
The Journal of clinical investigation123(11)4909-17(2013 Nov)
Song YQ,Karasugi T,Cheung KM,Chiba K,Ho DW,Miyake A,Kao PY,Sze KL,Yee A,Takahashi A,Kawaguchi Y,Mikami Y,Matsumoto M,Togawa D,Kanayama M,Shi D,Dai J,Jiang Q,Wu C,Tian W,Wang N,Leong JC,Luk KD,Yip SP,Cherny SS,Wang J,Mundlos S,Kelempisioti A,Eskola PJ,Männikkö M,Mäkelä P,Karppinen J,Järvelin MR,O'Reilly PF,Kubo M,Kimura T,Kubo T,Toyama Y,Mizuta H,Cheah KS,Tsunoda T,Sham PC,Ikegawa S,Chan D


book

Persistent cortical plasticity by upregulation of chondroitin 6-sulfation.
Nature neuroscience15(3)414-22, S1-2(2012 Jan)
Miyata S,Komatsu Y,Yoshimura Y,Taya C,Kitagawa H


book

Image-based genome-wide siRNA screen identifies selective autophagy factors.
Nature480(7375)113-7(2011 Dec)
Orvedahl A,Sumpter R Jr,Xiao G,Ng A,Zou Z,Tang Y,Narimatsu M,Gilpin C,Sun Q,Roth M,Forst CV,Wrana JL,Zhang YE,Luby-Phelps K,Xavier RJ,Xie Y,Levine B


 
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