LOC100202200 cDNA ORF clone, Hydra vulgaris

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100202200
Entrez Gene ID	100202200
Full Name	isoleucine--tRNA ligase, cytoplasmic
Synonyms	iars
Gene Type	protein-coding
Organism	Hydra vulgaris

mRNA and Protein(s)

mRNA	Protein	Name
XM_004208576.2	XP_004208624.2	isoleucine--tRNA ligase, cytoplasmic

Pathways from BioSystems
Gene Ontology

The following LOC100202200 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100202200 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHc14707	XM_004208576.2 Latest version!	Hydra vulgaris isoleucine--tRNA ligase, cytoplasmic (LOC100202200), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100202200 ( XM_004208576.2 ) cDNA ORF clone, Hydra vulgaris -> XP_004208624.2 Hydra vulgaris isoleucine--tRNA ligase, cytoplasmic (LOC100202200), mRNA.

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CloneID	OHc14707
Clone ID Related Accession (Same CDS sequence)	XM_004208576.2
Accession Version	XM_004208576.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-07
Organism	Hydra vulgaris
Product	isoleucine--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004168699.1) and transcript sequence (GAOL01021802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 8, 2015 this sequence version replaced XM_004208576.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hydra vulgaris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1251 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTAGTTG CTTTCAATCG TATAAGACCA AGAATATCTC TCTCTCTTAG CTCTACTGCC 
TTAAAGTCAT CTAGAAAAAT GTCTAATCCG CAAGGGGAGA CGCATAATTT TCCCAAAGAA 
GAAGAGAAGG TTTTAGAGTA TTGGAAGAAA ATTGATGCAT TCAAAACATC TTTGAAGTTA 
TCCGAAGGTA AACCGAGATT TACTTTTTAT GATGGACCTC CATTTGCTAC TGGACTACCC 
CATTATGGGC ATATATTGGC TGGGTCAATT AAGGATGTTG TAACTCGTTA TGCTCACCAA 
TCAGGGTTTC ACGTAGAAAG AAGGTTTGGA TGGGATTGCC ATGGCTTACC AGTTGAATTC 
GAAATCGATC AGAAACTTGG AATAAAGGGA CCTGATGATG TACAGAAAAT GGGTATAACT 
GCATACAATA AAGAGTGCAG GGCGATTGTA ATGAGATATG CAAAGGAATG GGAGAATGTT 
GTTTCTCGTC TTGGGAGATG GATCGATTTC GAAAACGATT ACAAAACTTT GTATCCATCA 
TTTATGGAAT CAGTATGGTG GGTGTTTAAG CAACTGTTTG AAAAAGGAAT GGTATACAGA 
GGATTTAGAG TTATGCCCTA TTCAACAAAA TGCAATACTC CACTTTCAAA TTTTGAAGCC 
AACCAAAACT ACAAAGATGT TATTGATCCT ACTGTTATTG TGAGTTTTCC ACTGGATGAA 
TCTCCGGACG TTAGCATGAT TGCCTGGACA ACTACTCCTT GGACATTACC TAGCAATTTG 
GCTCTTGTTG TCAACCCAGA TTTTATGTAT GTAAAAGTAA AAGATAATTC TAATGAAAAA 
GTGTACATTA TGATGGAAGA TAGACTGGAA TCATTATTTA AGAATAATAC AGAGTACACT 
ATCATTAGCA GATTTCCAGG AAGAGATTTG AAAGGAAAGA CCTACAAGCC TTTATTTCCG 
TATTTTGAAA AGCTTAAGTC AAGTGATGGA GCTTTTCAAA TACTTGTTGA TGGTTATGTG 
ACAAATGATA GTGGTACTGG AATCGTACAT TCGGCTCCTG CCTTTGGAGA GGATGATTAT 
CGTGTTTGTT TGGAAAATGG AATTTTAAAG CGCGGGGGTA AACTACCATG TCCAGTTAAT 
GAATCAGGTA TTTTTACCGA AGAAGTTGAA GATTTTAAGA ATATCTATGT TAAGGATGCT 
GATAAACTAA TCGCAAAGAA GTTAAAAGAA TTAGGTCGAT TGGTCCACCA AGGCAACATG 
AAACATAGTT ATCCATTCTG TTGGAGATCA GATACTCCAC TTATCTACAA AGCTGTTCCC 
GGTTGGTTTA TTCGTGTTGA GAATATTGTT CCAAATCTAT TAAAAAATAA TCAAGATTCT 
TATTGGGTTC CTGAGTTTAT AAAGGAGAAA CGCTTTCACA ATTGGTTAGA AAATGCAAGA 
GATTGGAATG TTTCAAGAAA TCGATACTGG GGTACCCCTA TGCCATTGTG GGCGAGTGAA 
GATTTTGAAG AGGTAATTTG TATCGGTTCA GTTGATGAAC TTCACAAGCT TTCTGGTATT 
AAAATATCTG ATCTTCATAG AGAAAATGTT GATAACATAA CAATACCTTC ATCAAAAGGT 
AATGGTACTT TAAAAAGGGT TCCAGAAGTA TTTGACTGTT GGTTTGAAAG TGGCAGTATG 
CCATATGCTC AAGCACATTA TCCATTTGAA AACAAAGAGG AATTTGAAAA GTCGTTTCCT 
GCTGATTTTA TAGCTGAAGG AGTTGATCAA ACTCGTGGTT GGTTTTATAC ATTGTTAGTT 
ATATCAACTC ATTTGTTCAA CAAACCGCCA TTCAAGAATC TTATTGTAAA TGGTCTTGTT 
TTAGCCGCAG ATGGAGAGAA GATGAGTAAA CGAAAGAAAA ACTATCCAGA TCCTCTATCT 
GTCATTGATA AATATGGTGC AGATGCATTA AGACTGTATC TTATTAACTC CCCAGTTGTT 
CGAGGTGAAA CTCTAAAGTT TCGAGAAGAA GGAGTACGTG ACATTATCAA GGATGTGTTT 
TTACCATGGT TTAATGCCTA TAGATTTTTA ATGCAGAATA TACGAGTTCA GGAAAAAGAT 
CATGCTTTTA AATTTGTTTT TGATCCATCA AAAGTTGAAC GTTCAGACAA TATTATGGAT 
CGTTGGATTA TATCGTTTAC GCAAAGTCTT TTGCTTTTTA TTAAGCAAGA AATGGCGGCT 
TATCGTCTTT ACACTGTTGT TCCTCGTTTA ATGAAGTTTA TTGATAATTT AACTAATTGG 
TATGTGCGTT CAAACAGGAA GCGTTTAAAG GGTGAATCCG GACCTACAGA TGGTTTACAA 
GCACTGCAGA CATTGTTTAA TGTAGTTTAT ACAATGGTAG GCGTGATGGC TCCTTATACT 
CCGTATTTAA CAGAACATAT GTATTTGAAT CTTAAAGGTT TTCTTGTTGA AGATGGTGTT 
GATCCAAAAA CTAAAGAAAG TGTTCATTAT CTAATGTTGC CACAACCTAG AGAAGATTTG 
ATTTTCTTGG ATGTTGAACG AGCTGTGTCT CGAATGCAAA CGGTTATAGA GTTAGGAAGG 
GTCACTCGTG ATAGAAAAAC ATTGCCTTTA AAATATCCAC TTCCATCCAT TGTAATTCTT 
CACAGTGATG AACAATACAT GAAAGATATT TTATCATTGC AGCATTACAT TCGTGAGGAA 
CTGAATGTAA AATTAGTAAC TACTTCCAAT GATAAAGAAT TATATGGACT TCGACTAAAA 
GCTGATTTAG ATTTTCAAGC TCTTGGAAAG CGTCTAAAAC AAGACTTTAA GAAAGTTCAA 
GAAGCCATTA ATGATGTTAC TGAAGATGAA CTCAAAGCAT TTAAAACTGT TGGTAAACTT 
CTTGTTGCTG GACATGAGTT ATTTGAAAAT GAAGTTAAGA TTAACTATTC CTTTGTTAAC 
AACAAAACAA CCAATATGTT TGAACCACAT TCCGACGGAG AGGTAGTAAT TTTATTAGAT 
ACAACTCCTG ATGAATCAAT GTTAGATGAA GGAATAGCAA GAGAAATAAT TAATCGAATG 
CAAAAGTTGC GGAAAAAGGC TGGTCTTGTT CCTACTGAAG AGATAACAGT AGTTTTCGAA 
ATTATTGCAG ATAAAGATGT TTCCGCATTT GAAAAACTTA GTTTAGTTGC AAAATCGCAT 
TTAAATTATA TGGTTGATAG CATTAAACAG CCTGTTGTCC TTGCACCAGG GCCTTCTTTA 
CTAGAAGAAA TTATAAATGA AGTAGTTGAC TGTAAAGGAG CAAAACTCAA ACTAAAAATT 
TTACGTGGTC GCCAAACAGA TAATTTAAAC AGAATAGAAC CTTATTGTAG ATTTATAAAT 
ATAGAATTAG TCCATAGTTG CGGAGAAACT GCTAAATCTA ATCATGGAAC ATTATTATTA 
GAAAATCCCT TTGGTAACTG TTTTTTAACG AAAGCGGAAA TGTTGAAACA GATCAATAAT 
ATATTTGGCA TTAATGGTTC ATACGTAGTT TCACCAAGTC CAGACCTTAA ACAAGATATC 
GAAGAACCTT TAACTAAATA CCACGGAAAG ACACTGTACG TGGGCAAATC CACTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004208624.2
CDS	69..3668
Translation	MLVAFNRIRPRISLSLSSTALKSSRKMSNPQGETHNFPKEEEKVLEYWKKIDAFKTSLKLSEGKPRFTFYDGPPFATGLPHYGHILAGSIKDVVTRYAHQSGFHVERRFGWDCHGLPVEFEIDQKLGIKGPDDVQKMGITAYNKECRAIVMRYAKEWENVVSRLGRWIDFENDYKTLYPSFMESVWWVFKQLFEKGMVYRGFRVMPYSTKCNTPLSNFEANQNYKDVIDPTVIVSFPLDESPDVSMIAWTTTPWTLPSNLALVVNPDFMYVKVKDNSNEKVYIMMEDRLESLFKNNTEYTIISRFPGRDLKGKTYKPLFPYFEKLKSSDGAFQILVDGYVTNDSGTGIVHSAPAFGEDDYRVCLENGILKRGGKLPCPVNESGIFTEEVEDFKNIYVKDADKLIAKKLKELGRLVHQGNMKHSYPFCWRSDTPLIYKAVPGWFIRVENIVPNLLKNNQDSYWVPEFIKEKRFHNWLENARDWNVSRNRYWGTPMPLWASEDFEEVICIGSVDELHKLSGIKISDLHRENVDNITIPSSKGNGTLKRVPEVFDCWFESGSMPYAQAHYPFENKEEFEKSFPADFIAEGVDQTRGWFYTLLVISTHLFNKPPFKNLIVNGLVLAADGEKMSKRKKNYPDPLSVIDKYGADALRLYLINSPVVRGETLKFREEGVRDIIKDVFLPWFNAYRFLMQNIRVQEKDHAFKFVFDPSKVERSDNIMDRWIISFTQSLLLFIKQEMAAYRLYTVVPRLMKFIDNLTNWYVRSNRKRLKGESGPTDGLQALQTLFNVVYTMVGVMAPYTPYLTEHMYLNLKGFLVEDGVDPKTKESVHYLMLPQPREDLIFLDVERAVSRMQTVIELGRVTRDRKTLPLKYPLPSIVILHSDEQYMKDILSLQHYIREELNVKLVTTSNDKELYGLRLKADLDFQALGKRLKQDFKKVQEAINDVTEDELKAFKTVGKLLVAGHELFENEVKINYSFVNNKTTNMFEPHSDGEVVILLDTTPDESMLDEGIAREIINRMQKLRKKAGLVPTEEITVVFEIIADKDVSAFEKLSLVAKSHLNYMVDSIKQPVVLAPGPSLLEEIINEVVDCKGAKLKLKILRGRQTDNLNRIEPYCRFINIELVHSCGETAKSNHGTLLLENPFGNCFLTKAEMLKQINNIFGINGSYVVSPSPDLKQDIEEPLTKYHGKTLYVGKSTK

Target ORF information:

RefSeq Version	XM_004208576.2
Organism	Hydra vulgaris
Definition	Hydra vulgaris isoleucine--tRNA ligase, cytoplasmic (LOC100202200), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004208576.2

ORF Insert Sequence:

ATGCTAGTTG CTTTCAATCG TATAAGACCA AGAATATCTC TCTCTCTTAG CTCTACTGCC 
TTAAAGTCAT CTAGAAAAAT GTCTAATCCG CAAGGGGAGA CGCATAATTT TCCCAAAGAA 
GAAGAGAAGG TTTTAGAGTA TTGGAAGAAA ATTGATGCAT TCAAAACATC TTTGAAGTTA 
TCCGAAGGTA AACCGAGATT TACTTTTTAT GATGGACCTC CATTTGCTAC TGGACTACCC 
CATTATGGGC ATATATTGGC TGGGTCAATT AAGGATGTTG TAACTCGTTA TGCTCACCAA 
TCAGGGTTTC ACGTAGAAAG AAGGTTTGGA TGGGATTGCC ATGGCTTACC AGTTGAATTC 
GAAATCGATC AGAAACTTGG AATAAAGGGA CCTGATGATG TACAGAAAAT GGGTATAACT 
GCATACAATA AAGAGTGCAG GGCGATTGTA ATGAGATATG CAAAGGAATG GGAGAATGTT 
GTTTCTCGTC TTGGGAGATG GATCGATTTC GAAAACGATT ACAAAACTTT GTATCCATCA 
TTTATGGAAT CAGTATGGTG GGTGTTTAAG CAACTGTTTG AAAAAGGAAT GGTATACAGA 
GGATTTAGAG TTATGCCCTA TTCAACAAAA TGCAATACTC CACTTTCAAA TTTTGAAGCC 
AACCAAAACT ACAAAGATGT TATTGATCCT ACTGTTATTG TGAGTTTTCC ACTGGATGAA 
TCTCCGGACG TTAGCATGAT TGCCTGGACA ACTACTCCTT GGACATTACC TAGCAATTTG 
GCTCTTGTTG TCAACCCAGA TTTTATGTAT GTAAAAGTAA AAGATAATTC TAATGAAAAA 
GTGTACATTA TGATGGAAGA TAGACTGGAA TCATTATTTA AGAATAATAC AGAGTACACT 
ATCATTAGCA GATTTCCAGG AAGAGATTTG AAAGGAAAGA CCTACAAGCC TTTATTTCCG 
TATTTTGAAA AGCTTAAGTC AAGTGATGGA GCTTTTCAAA TACTTGTTGA TGGTTATGTG 
ACAAATGATA GTGGTACTGG AATCGTACAT TCGGCTCCTG CCTTTGGAGA GGATGATTAT 
CGTGTTTGTT TGGAAAATGG AATTTTAAAG CGCGGGGGTA AACTACCATG TCCAGTTAAT 
GAATCAGGTA TTTTTACCGA AGAAGTTGAA GATTTTAAGA ATATCTATGT TAAGGATGCT 
GATAAACTAA TCGCAAAGAA GTTAAAAGAA TTAGGTCGAT TGGTCCACCA AGGCAACATG 
AAACATAGTT ATCCATTCTG TTGGAGATCA GATACTCCAC TTATCTACAA AGCTGTTCCC 
GGTTGGTTTA TTCGTGTTGA GAATATTGTT CCAAATCTAT TAAAAAATAA TCAAGATTCT 
TATTGGGTTC CTGAGTTTAT AAAGGAGAAA CGCTTTCACA ATTGGTTAGA AAATGCAAGA 
GATTGGAATG TTTCAAGAAA TCGATACTGG GGTACCCCTA TGCCATTGTG GGCGAGTGAA 
GATTTTGAAG AGGTAATTTG TATCGGTTCA GTTGATGAAC TTCACAAGCT TTCTGGTATT 
AAAATATCTG ATCTTCATAG AGAAAATGTT GATAACATAA CAATACCTTC ATCAAAAGGT 
AATGGTACTT TAAAAAGGGT TCCAGAAGTA TTTGACTGTT GGTTTGAAAG TGGCAGTATG 
CCATATGCTC AAGCACATTA TCCATTTGAA AACAAAGAGG AATTTGAAAA GTCGTTTCCT 
GCTGATTTTA TAGCTGAAGG AGTTGATCAA ACTCGTGGTT GGTTTTATAC ATTGTTAGTT 
ATATCAACTC ATTTGTTCAA CAAACCGCCA TTCAAGAATC TTATTGTAAA TGGTCTTGTT 
TTAGCCGCAG ATGGAGAGAA GATGAGTAAA CGAAAGAAAA ACTATCCAGA TCCTCTATCT 
GTCATTGATA AATATGGTGC AGATGCATTA AGACTGTATC TTATTAACTC CCCAGTTGTT 
CGAGGTGAAA CTCTAAAGTT TCGAGAAGAA GGAGTACGTG ACATTATCAA GGATGTGTTT 
TTACCATGGT TTAATGCCTA TAGATTTTTA ATGCAGAATA TACGAGTTCA GGAAAAAGAT 
CATGCTTTTA AATTTGTTTT TGATCCATCA AAAGTTGAAC GTTCAGACAA TATTATGGAT 
CGTTGGATTA TATCGTTTAC GCAAAGTCTT TTGCTTTTTA TTAAGCAAGA AATGGCGGCT 
TATCGTCTTT ACACTGTTGT TCCTCGTTTA ATGAAGTTTA TTGATAATTT AACTAATTGG 
TATGTGCGTT CAAACAGGAA GCGTTTAAAG GGTGAATCCG GACCTACAGA TGGTTTACAA 
GCACTGCAGA CATTGTTTAA TGTAGTTTAT ACAATGGTAG GCGTGATGGC TCCTTATACT 
CCGTATTTAA CAGAACATAT GTATTTGAAT CTTAAAGGTT TTCTTGTTGA AGATGGTGTT 
GATCCAAAAA CTAAAGAAAG TGTTCATTAT CTAATGTTGC CACAACCTAG AGAAGATTTG 
ATTTTCTTGG ATGTTGAACG AGCTGTGTCT CGAATGCAAA CGGTTATAGA GTTAGGAAGG 
GTCACTCGTG ATAGAAAAAC ATTGCCTTTA AAATATCCAC TTCCATCCAT TGTAATTCTT 
CACAGTGATG AACAATACAT GAAAGATATT TTATCATTGC AGCATTACAT TCGTGAGGAA 
CTGAATGTAA AATTAGTAAC TACTTCCAAT GATAAAGAAT TATATGGACT TCGACTAAAA 
GCTGATTTAG ATTTTCAAGC TCTTGGAAAG CGTCTAAAAC AAGACTTTAA GAAAGTTCAA 
GAAGCCATTA ATGATGTTAC TGAAGATGAA CTCAAAGCAT TTAAAACTGT TGGTAAACTT 
CTTGTTGCTG GACATGAGTT ATTTGAAAAT GAAGTTAAGA TTAACTATTC CTTTGTTAAC 
AACAAAACAA CCAATATGTT TGAACCACAT TCCGACGGAG AGGTAGTAAT TTTATTAGAT 
ACAACTCCTG ATGAATCAAT GTTAGATGAA GGAATAGCAA GAGAAATAAT TAATCGAATG 
CAAAAGTTGC GGAAAAAGGC TGGTCTTGTT CCTACTGAAG AGATAACAGT AGTTTTCGAA 
ATTATTGCAG ATAAAGATGT TTCCGCATTT GAAAAACTTA GTTTAGTTGC AAAATCGCAT 
TTAAATTATA TGGTTGATAG CATTAAACAG CCTGTTGTCC TTGCACCAGG GCCTTCTTTA 
CTAGAAGAAA TTATAAATGA AGTAGTTGAC TGTAAAGGAG CAAAACTCAA ACTAAAAATT 
TTACGTGGTC GCCAAACAGA TAATTTAAAC AGAATAGAAC CTTATTGTAG ATTTATAAAT 
ATAGAATTAG TCCATAGTTG CGGAGAAACT GCTAAATCTA ATCATGGAAC ATTATTATTA 
GAAAATCCCT TTGGTAACTG TTTTTTAACG AAAGCGGAAA TGTTGAAACA GATCAATAAT 
ATATTTGGCA TTAATGGTTC ATACGTAGTT TCACCAAGTC CAGACCTTAA ACAAGATATC 
GAAGAACCTT TAACTAAATA CCACGGAAAG ACACTGTACG TGGGCAAATC CACTAAATGA