LOC8031660 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8031660
Entrez Gene ID	8031660
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029983249.1	XP_029839109.1	SR-related and CTD-associated factor 8 isoform X3
XM_002406655.1	XP_002406699.1	hypothetical protein
XM_029983247.1	XP_029839107.1	SR-related and CTD-associated factor 8 isoform X1
XM_029983248.1	XP_029839108.1	SR-related and CTD-associated factor 8 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC8031660 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8031660 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa04787	XM_002406655.1 Latest version!	Ixodes scapularis hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OIa25776	XM_029983247.1 Latest version!	Ixodes scapularis SR-related and CTD-associated factor 8 (LOC8031660), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa25777	XM_029983248.1 Latest version!	Ixodes scapularis SR-related and CTD-associated factor 8 (LOC8031660), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa25778	XM_029983249.1 Latest version!	Ixodes scapularis SR-related and CTD-associated factor 8 (LOC8031660), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8031660 ( XM_002406655.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002406699.1 Ixodes scapularis hypothetical protein, mRNA.

Price & Availability↑

CloneID	OIa04787
Clone ID Related Accession (Same CDS sequence)	XM_002406655.1
Accession Version	XM_002406655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2009-06-22
Organism	Ixodes scapularis(black-legged tick)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002688148). COMPLETENESS: incomplete on both ends.

ATGGCCTGGG CCCCTGGCAA GGGCGTGAAG AGCAAGGAGC TGAAGGACTT CTGGGAGGTT 
GACCTCGGCG TGAGCTACAT CCCGTACGAG AAGCTGCCCA AGGACGTCGA CCTCGTGTCC 
CTGGAAGAGG GCGGAGTCAT CGATGAGGAC TCTCTTCCCG AGGACCTCAG AGAGAAGCGG 
AGGGCCCAGG AACAGCAGCG CACTCAGGGT GGGCCCGGTC TGGCACCCTC CACGGTGGCC 
GGCGGCGGCG GTGCCGACGT GGTGGCCGCA ATGGCAGCCT TTCCGGGGGC TGCCATGACC 
ACCCCAGTTG TGACCACCAT GGCTGCCCCC GGGGGTGCCG CCACTGCGGT GGCCATGGTG 
CCGCCGCCCG TGGCACAATT TGGGATCCCG TTGCCACCAG GCAACGTTCG GGCGACTGCC 
GAGGCAGTGC GACTCGACGG CACTTTGCGT CGCATCCAAG CACGTTGCGG TAAGGATTCG 
GACGCGATCG GCTCGTCACT CGGAGGGACG ACGGAGGACC TCGGCCCCGG CAATCGCGCC 
TTCGAAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002406699.1
CDS	1..552
Translation	MAWAPGKGVKSKELKDFWEVDLGVSYIPYEKLPKDVDLVSLEEGGVIDEDSLPEDLREKRRAQEQQRTQGGPGLAPSTVAGGGGADVVAAMAAFPGAAMTTPVVTTMAAPGGAATAVAMVPPPVAQFGIPLPPGNVRATAEAVRLDGTLRRIQARCGKDSDAIGSSLGGTTEDLGPGNRAFES

Target ORF information:

RefSeq Version	XM_002406655.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002406655.1

ORF Insert Sequence:

ATGGCCTGGG CCCCTGGCAA GGGCGTGAAG AGCAAGGAGC TGAAGGACTT CTGGGAGGTT 
GACCTCGGCG TGAGCTACAT CCCGTACGAG AAGCTGCCCA AGGACGTCGA CCTCGTGTCC 
CTGGAAGAGG GCGGAGTCAT CGATGAGGAC TCTCTTCCCG AGGACCTCAG AGAGAAGCGG 
AGGGCCCAGG AACAGCAGCG CACTCAGGGT GGGCCCGGTC TGGCACCCTC CACGGTGGCC 
GGCGGCGGCG GTGCCGACGT GGTGGCCGCA ATGGCAGCCT TTCCGGGGGC TGCCATGACC 
ACCCCAGTTG TGACCACCAT GGCTGCCCCC GGGGGTGCCG CCACTGCGGT GGCCATGGTG 
CCGCCGCCCG TGGCACAATT TGGGATCCCG TTGCCACCAG GCAACGTTCG GGCGACTGCC 
GAGGCAGTGC GACTCGACGG CACTTTGCGT CGCATCCAAG CACGTTGCGG TAAGGATTCG 
GACGCGATCG GCTCGTCACT CGGAGGGACG ACGGAGGACC TCGGCCCCGG CAATCGCGCC 
TTCGAAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8031660 ( XM_029983247.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029839107.1 Ixodes scapularis SR-related and CTD-associated factor 8 (LOC8031660), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa25776
Clone ID Related Accession (Same CDS sequence)	XM_029983247.1
Accession Version	XM_029983247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	SR-related and CTD-associated factor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021920090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTTG TTAAAGCCTT CAACGGCGAG CTTTCCTCGC TGTATGAAGG CAAGCCTCCC 
ATCTCGAAAG CCAAGATGAC AGCAATTACA AAGTCTGCCA TCAAGGGCAT CAAATTCTAC 
AAACACATAG TTCAGAGCGT GGAGAAGTTT GTTCAGAAGT GCCGGCCCGA GTACAAGGTT 
CCAGGCCTGT ACGTTGTGGA CTCGATTGTG CGCCAGTCGC GGCACCAGTT TGGCCAGGAG 
AAGGATGTGT TCGCACCCCG GTTCACCAAG AACATCCACA ACACCTTCCA GCACCTCTTC 
AAGTGCCCCG TGGAGGACAG ACCCAAGATT GTGCGGGTGC TCAATTTGTG GCAGAAGAAC 
CAGGTGTTCC CCCCGGATGT CATCCAGCCG CTTTTGGACA TGGCGAGCCC CAGTGCGGTG 
TTGGCGGCAG GGGACATGAG CGAGGGGCCC GACATGGGCC TGGACTCAAA GCCCCCCAGC 
AAGGCGGCGA TGGACCAGAT GGCCTCCTGG AAGGGCTGGA ACCTGGGAGG CAACAGCGTC 
CAGGACAATG CCTCCGCCCC TCCACAGGAG ACCAAGACCT ACGCCCTGAC GCAGGAGAAC 
TTGAACCTGG CCCTGGCGGC AGAGGCGGAA GGCGCGAGCG CCCCGGACCC CGAGATGCTG 
GTGAAGCTGC AGAGCCTGGC GTCGCAGCTG CTGGGGGCGA GAGCGCTGGG AAGCGGGAAC 
GCCATTGCCG AAGAGAAGCC CAGTCAGCCC TCGGCCCCGG CCGTCAAGTT CAACAAGAAA 
CTGCTGGACT TTGACTACGG GGACGAAGAG GAAGAGGAGG AGGAGGAACC CGTGGAGGAA 
ACAAGCACCC TGGCCCCGCC CGAGACCAAC CTGGAGGACT CCGGAGGAAG GCCGTCAGAA 
CCCAATGCTC TGGCACTCAG CATGGCTCAG AACCTGCTGA ACAATCCAGA GTTGCTGAAG 
CAGCTGCAGC AGATGCAGCA GACCCTTCAG CAGACGGAGG TGCTTAGGGG CTCGGAGACG 
TCGGCCGCCC CCGCAGCCCC TTCGTCGCTC GGAACGGCCG CGCTCCCCCA GTGCTCGGAG 
ACTGGCAAGG CGCCCTCCCC TCATCCGCAG ACCCCGCAGG GGCCGGACGG GCAGCCGCTG 
GCGACGACTG CGGGGTACCT GACGGCCTCG GCCTCGGTGG TGAACTCCCT GGCGCCTGCG 
GTGAGCTCTG GGCTTTCCCA GCTTTTCCAG ACAGACTTTG CGGGGGCCTC TGGTGCTCCT 
TTGCTGCCTG AATCTCTGAG CCAGCTGAAC ATGCCCCTTT ACCCGCCGGC GATGGCGATG 
CCTCAGGGGG TCCCCTATGG GGCCGGTGCA ATAGGGCCGG ACGCCGTGTC CCAGGGGTAC 
CCGCCGGCCC CCCAAGGTGT GCTGGCCCCC CCCATGGGCG AGCTCTCCGG CCTGGAGGGC 
GTGCCCATGC CCCCCGCGGC CGGCGTGGCC CCGCCCGCGT ACCTGAGTGG GGGCGACATG 
GACGAGCGCT GCACTGTCTT GGCGCCCGGC GGAAGGGACC TGGACCTCAG GACAGGGGGT 
CTGTCCCCTC CCAGGGACAG AGATAGGCGG CGGCACAGTC GTTCCCGCTC TCCTCGGAGG 
CGTGGCCGTT CCCGGTCTCG GTCGCCCTCC CAGCGCACCA CGCCGGGTGC CCGGTACAAC 
GCCGCCAAGC GGGACCGGTC ACGCGAGCGG GAGAAGGAGC GGGAGCGGGA GAGGGACCGC 
CGGCGCAAGG GCCTGCCCCC CGTCAAGAAG GACTGCCTCA GCGTGTGCAG CTCGACCTTG 
TGGTTGGGAC ACGTGCCCAA GTCCGTGTCG GAGACGGACC TTCACGACAC CTTCGGCGAA 
TTTGGAAGCA TCGTCAAGAT TGAGATTATC GCCCCTCGTG GCTGTGCATT CGTGTGCATG 
GACCGGCGGC AGGATGCCTT TCGTGCCCTG CAGAAACTCG CAAAAGCACT CAAGCTTCAG 
GGGAGCCTCA TCAAGATGGC CTGGGCCCCT GGCAAGGGTG TGAAGAGCAA GGAGCTGAAG 
GACTTCTGGG AGGTTGACCT CGGCGTGAGC TACATCCCGT ACGAGAAGCT TCCCAAGGAC 
GTCGACCTCG TGTCCCTGGA AGAGGGCGGA GTCATCGACG AGGACTCTCT TCCTGAGGAC 
CTCAGAGAGA AGCGGAGGGC CCAGGAACAG CAGCGCACTC AGGGTGGGCC CGGTCTGGCA 
CCCTCCACGG CGGCCGGCGG CGGCGGTGCC GACGTGGTGG CCGCAATGGC AGCCTTTCCG 
GGGGCCGCCA TGACCACCCC AGTGGTGACC ACCATGGCTG CCCCCGGGGG TGCCGCCACT 
GCGGTGGCCA TGGTGCCGCC GCCCGTGGCA CAGTTTGGGA TCCCGCTGCC ACCAGTTGTC 
CAAGTTAAAC CGAGCGAACC TGAAATAAAA GGTGCATGCA TGCTGCCCTC GCAGGGCTTG 
GTGATGCCGC AGATACCAGT GCCCATGGGG GTGCCCGCCC CTCCAAACCA CATGCTGATG 
GTGCCACCCC ACGGCACCCA AACATTGCTG GGCCACAGTG CACTGGGACT CCTCGGTCCA 
CCGCCATTGG GAATCTCAGG AACTGCCCTG GGCCTTTCCC TGCACCCTCC CATGAGCCTC 
CCGACCACCT CCCAGTCTGC CCCTGTGCTG CCCGTGGCAA CAACAGCACA GCTGATGGCG 
GCGGCGGCAG CTGCGGCGGC CACGACGGCT ACGGTGACGA CCGACACCAC TGCGACCACT 
GCGACGCCCA TGGCCATCGA CGGGGATGCA ACGCCGACAG ACGAGGATGG AACGAGTGTC 
ATGGACAGCG TCAGCGCCAT TCCTACTCCG GCGGACCCTC CGGAACAACA AGGCGAGGCG 
TCCAAGGATT CAGTGTTTGG GAAACTTCTG CCACCACCAA TGTCTTTTCC GCCTCTCGCT 
GCGCCTCCAG GAGTTCCTTC GCAGTTCGTC ACTCAGGGAC TGGTGGGTGC TCAGAAGGAA 
CCGCACCCTG CCGAAGCTAA GTTCAAGTCT CCGGGATCTA GTCAAGAAAC TTCTAGTGGA 
AATGTGCAGG GCGCTGGCAT CTCTCAAGAG AAGCCACCAC CGGAAGGCAT GCAGATTGAG 
GACTCCAACT CCCAGGAACA GCAGAGCGAC TCAAGGACGG AGATACGCAG CTTCGAGACG 
TCACCCTCGG CGATACCTTT GCCGGTCGGA CTTCAGGGCT TTAACTCCTC GCCGATGCCT 
CCAAGAGGAC TGGGGGGACC TCCCATACGC TTCAGAGGAC CGCCCCTATT TCCGTTTGAT 
GGTCCACCGA TGCCCGATGG AAGACCTGGA ATGGACTTTC CAGGTCCGAG ACTGCTGGGG 
CCCGATCCTA GGATGATGGG ACCGCCTTCG GACTTCAGAC AGGGAAGCCC TCATGAGCAT 
CGAATGTTGG GTGGTCCCAT GGAGCAGCGG ATGATGGGTG GGGCAGAACA GCCGATGATG 
CGAGGTCCCA TGGAGCAAAG GCTGTTGGGC CCTCCAGAAC AGATGATGGG TGGGCCTCCG 
GACTCGCGGA TGTTGAGGGG TCCCCCAGAG CAAAGGTTTC TGGGCATGCC GCAGGAGCCA 
AGGATGCTGG GAGGTCCTTT GTCACCAAGA ATGCTAGGGG GTCCTCCGGA TGATAGACTA 
ATGGGTGGTC CACCAGATCA GAGGATGATG GGAGGACCAC GGGAGCACAG AATGTTGGGC 
GGTCCACCTC ACGAGCATAG AATGATGGGT GGTCCTCCTG AACACAGAAT GATGGGTGGG 
CCCCCAGACC ACCGGATGTT GGGTGGGCCC CTGGATCATA GGATGATGGG TGGGCCCCCT 
GACCACCGAA TGTTGGGTGG ACCGCTGGAC CACAGAATGA TGGGAGGCCC GCCAGACCAT 
AGGATGTTAA ACATTCCTCC GGAGCACAGG ATGATGGGCA GGCCAGGAGA TTTAGAACAC 
CGGATGATGG GAGGTCCCCC TGAACACCGG ATGAGGGGGG GTCCACCAGA CCATCGGATG 
ATGGGAGGTC CTCCGGATCA TAGGATGATG GGCGGGCCTC CCGACCACAG GATGATGGGC 
AGTCCACCTG AACACAGAAT GCTTCCAGGA CCTCAAGACC AGAGGATGAT GGGAGGTCCA 
CCGGACCACC GAATGCTCGG ACCTCCCGAT TTCATGATGG GTGGACCGCC GGAGTTTCGA 
AAGCTTGGAC CGCCGGACTC GCGAATGTTG GGACCGGGAC CTCGAATGCT GGGACCCAAT 
TTCGGCATGA TGAGCCCGAA TCAGATGGGG CCCCATGGTC CAGGCGGAGC AGACTCGGAC 
CCGGCAGATA AGATGGCAAA TTCTGATGGC AAGAAGGTGG CAACTGACAA AGAGGAAGGC 
AATGCCGGTG AAGGTGGTAA GGACGCGCAC TCGGGCGATA GGGAGGCAAA TATGGACGAA 
AAGAAAAACA GTCAAAACGA GCCAACCAAG AACTGGAATC ACCAGGTGGG AGGCGCCGAA 
GACGGCCGTG TACCAAATTT AATGGACGGT GGGCTGGGAA GACCCGACGG CGACAAGGGT 
CCCCAGAGCG ACGGCGATCG CGACAGGAAA GGCTTTGGCT TTTTTGGGCC ACCCCGTACG 
AGGTTTGACG GTCCAGAAGA GCGGTCCGGC TTCAGACCTG CCTTCAACGA TGGGCCAATG 
GATCGCGGTT CCGATAACGC CTGGGGAGGC AGGGACGGCG AAGACGGCGG TGGGTTTGGC 
GGCGATCGCC GCGTTTTCAC GAATGCCTTC GGCGAAATGT CAGAAAGGCG GCGGAGCGGC 
GGTGGTGGCG GCGGCGGCAG TGGGGGCGGC AGTGGCGGTC GAGGGGAGCG TAGGAGCCGC 
TGGTCTCGGG AAGACAACGA GTGGGACTCG CGGCGTAGTG ATCGCGCAGA TCGCAGTGAT 
CGAAGCGATC GTGACCGAAG TGATCGGGAT CGGAACGACC GTGACCGCAG AGACCGAGAC 
CGTACCGACG GCAAAGACAG AAGCGACCGG GATCGCTCGG ACAAGGACCG TAACGATCGG 
GAAAGAAGTG ATCGGGATCG GAGCGACCGG GATCGAAGCG ATCGTGACCG TAGTGATCGG 
GACCGGAGTG ACCGAGACCG CAGCGAGAAA GATCGGGACC GGAGTGAAAA GGACCGTAGC 
GACAGGGATC GCAGCGACAA AGATCGCAGT GAAAAGGATC GCAGCGACAA GGACCGCGGT 
GACAGGGATC GAGACCGAAG CAAAGACCGG GACCGAAGCA AAGATCGGGA CCGTAGCAAA 
GATCGGGACC GGAGCAAAGA CCGAGATCGA AGTAGAGACC GCGATCGGAG TGATAGGGAT 
CGAGATCGGA GTGATAGAGA AAAGGGCGAC AAGGATCGTG AGCGTAGCGA CAAGGATCGT 
GAGCGCAGTG ATAAAGACCG TGGTGATCGT GATCGGAGTG AGCGTGATCG CAGCAAAGGG 
GATTACCGGA GAAGCCGCGA GACTGACCGG GAGCGGGAAC GGAGCTCAGG CCGGAGCCAC 
AGCTCGTTAG AACAAAGCTC ATCGGCTGTC GAGACCAACG GAGAGGTCTC CAAGGCCAAC 
GGGAGCAGCT CACAGTCGTC GAAAGAGGAC AAGGCGCTAG GCAAGGCGCT AGACAGGGCG 
CTGGACAATG TGCTGGACAA GGCTGAGGCA CCCAAGCCCC AGGAAGTGGA GGAGGTCAGT 
GGGACGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029839107.1
CDS	93..5804
Translation	MEVVKAFNGELSSLYEGKPPISKAKMTAITKSAIKGIKFYKHIVQSVEKFVQKCRPEYKVPGLYVVDSIVRQSRHQFGQEKDVFAPRFTKNIHNTFQHLFKCPVEDRPKIVRVLNLWQKNQVFPPDVIQPLLDMASPSAVLAAGDMSEGPDMGLDSKPPSKAAMDQMASWKGWNLGGNSVQDNASAPPQETKTYALTQENLNLALAAEAEGASAPDPEMLVKLQSLASQLLGARALGSGNAIAEEKPSQPSAPAVKFNKKLLDFDYGDEEEEEEEEPVEETSTLAPPETNLEDSGGRPSEPNALALSMAQNLLNNPELLKQLQQMQQTLQQTEVLRGSETSAAPAAPSSLGTAALPQCSETGKAPSPHPQTPQGPDGQPLATTAGYLTASASVVNSLAPAVSSGLSQLFQTDFAGASGAPLLPESLSQLNMPLYPPAMAMPQGVPYGAGAIGPDAVSQGYPPAPQGVLAPPMGELSGLEGVPMPPAAGVAPPAYLSGGDMDERCTVLAPGGRDLDLRTGGLSPPRDRDRRRHSRSRSPRRRGRSRSRSPSQRTTPGARYNAAKRDRSREREKERERERDRRRKGLPPVKKDCLSVCSSTLWLGHVPKSVSETDLHDTFGEFGSIVKIEIIAPRGCAFVCMDRRQDAFRALQKLAKALKLQGSLIKMAWAPGKGVKSKELKDFWEVDLGVSYIPYEKLPKDVDLVSLEEGGVIDEDSLPEDLREKRRAQEQQRTQGGPGLAPSTAAGGGGADVVAAMAAFPGAAMTTPVVTTMAAPGGAATAVAMVPPPVAQFGIPLPPVVQVKPSEPEIKGACMLPSQGLVMPQIPVPMGVPAPPNHMLMVPPHGTQTLLGHSALGLLGPPPLGISGTALGLSLHPPMSLPTTSQSAPVLPVATTAQLMAAAAAAAATTATVTTDTTATTATPMAIDGDATPTDEDGTSVMDSVSAIPTPADPPEQQGEASKDSVFGKLLPPPMSFPPLAAPPGVPSQFVTQGLVGAQKEPHPAEAKFKSPGSSQETSSGNVQGAGISQEKPPPEGMQIEDSNSQEQQSDSRTEIRSFETSPSAIPLPVGLQGFNSSPMPPRGLGGPPIRFRGPPLFPFDGPPMPDGRPGMDFPGPRLLGPDPRMMGPPSDFRQGSPHEHRMLGGPMEQRMMGGAEQPMMRGPMEQRLLGPPEQMMGGPPDSRMLRGPPEQRFLGMPQEPRMLGGPLSPRMLGGPPDDRLMGGPPDQRMMGGPREHRMLGGPPHEHRMMGGPPEHRMMGGPPDHRMLGGPLDHRMMGGPPDHRMLGGPLDHRMMGGPPDHRMLNIPPEHRMMGRPGDLEHRMMGGPPEHRMRGGPPDHRMMGGPPDHRMMGGPPDHRMMGSPPEHRMLPGPQDQRMMGGPPDHRMLGPPDFMMGGPPEFRKLGPPDSRMLGPGPRMLGPNFGMMSPNQMGPHGPGGADSDPADKMANSDGKKVATDKEEGNAGEGGKDAHSGDREANMDEKKNSQNEPTKNWNHQVGGAEDGRVPNLMDGGLGRPDGDKGPQSDGDRDRKGFGFFGPPRTRFDGPEERSGFRPAFNDGPMDRGSDNAWGGRDGEDGGGFGGDRRVFTNAFGEMSERRRSGGGGGGGSGGGSGGRGERRSRWSREDNEWDSRRSDRADRSDRSDRDRSDRDRNDRDRRDRDRTDGKDRSDRDRSDKDRNDRERSDRDRSDRDRSDRDRSDRDRSDRDRSEKDRDRSEKDRSDRDRSDKDRSEKDRSDKDRGDRDRDRSKDRDRSKDRDRSKDRDRSKDRDRSRDRDRSDRDRDRSDREKGDKDRERSDKDRERSDKDRGDRDRSERDRSKGDYRRSRETDRERERSSGRSHSSLEQSSSAVETNGEVSKANGSSSQSSKEDKALGKALDRALDNVLDKAEAPKPQEVEEVSGTA

Target ORF information:

RefSeq Version	XM_029983247.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis SR-related and CTD-associated factor 8 (LOC8031660), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029983247.1

ORF Insert Sequence:

ATGGAGGTTG TTAAAGCCTT CAACGGCGAG CTTTCCTCGC TGTATGAAGG CAAGCCTCCC 
ATCTCGAAAG CCAAGATGAC AGCAATTACA AAGTCTGCCA TCAAGGGCAT CAAATTCTAC 
AAACACATAG TTCAGAGCGT GGAGAAGTTT GTTCAGAAGT GCCGGCCCGA GTACAAGGTT 
CCAGGCCTGT ACGTTGTGGA CTCGATTGTG CGCCAGTCGC GGCACCAGTT TGGCCAGGAG 
AAGGATGTGT TCGCACCCCG GTTCACCAAG AACATCCACA ACACCTTCCA GCACCTCTTC 
AAGTGCCCCG TGGAGGACAG ACCCAAGATT GTGCGGGTGC TCAATTTGTG GCAGAAGAAC 
CAGGTGTTCC CCCCGGATGT CATCCAGCCG CTTTTGGACA TGGCGAGCCC CAGTGCGGTG 
TTGGCGGCAG GGGACATGAG CGAGGGGCCC GACATGGGCC TGGACTCAAA GCCCCCCAGC 
AAGGCGGCGA TGGACCAGAT GGCCTCCTGG AAGGGCTGGA ACCTGGGAGG CAACAGCGTC 
CAGGACAATG CCTCCGCCCC TCCACAGGAG ACCAAGACCT ACGCCCTGAC GCAGGAGAAC 
TTGAACCTGG CCCTGGCGGC AGAGGCGGAA GGCGCGAGCG CCCCGGACCC CGAGATGCTG 
GTGAAGCTGC AGAGCCTGGC GTCGCAGCTG CTGGGGGCGA GAGCGCTGGG AAGCGGGAAC 
GCCATTGCCG AAGAGAAGCC CAGTCAGCCC TCGGCCCCGG CCGTCAAGTT CAACAAGAAA 
CTGCTGGACT TTGACTACGG GGACGAAGAG GAAGAGGAGG AGGAGGAACC CGTGGAGGAA 
ACAAGCACCC TGGCCCCGCC CGAGACCAAC CTGGAGGACT CCGGAGGAAG GCCGTCAGAA 
CCCAATGCTC TGGCACTCAG CATGGCTCAG AACCTGCTGA ACAATCCAGA GTTGCTGAAG 
CAGCTGCAGC AGATGCAGCA GACCCTTCAG CAGACGGAGG TGCTTAGGGG CTCGGAGACG 
TCGGCCGCCC CCGCAGCCCC TTCGTCGCTC GGAACGGCCG CGCTCCCCCA GTGCTCGGAG 
ACTGGCAAGG CGCCCTCCCC TCATCCGCAG ACCCCGCAGG GGCCGGACGG GCAGCCGCTG 
GCGACGACTG CGGGGTACCT GACGGCCTCG GCCTCGGTGG TGAACTCCCT GGCGCCTGCG 
GTGAGCTCTG GGCTTTCCCA GCTTTTCCAG ACAGACTTTG CGGGGGCCTC TGGTGCTCCT 
TTGCTGCCTG AATCTCTGAG CCAGCTGAAC ATGCCCCTTT ACCCGCCGGC GATGGCGATG 
CCTCAGGGGG TCCCCTATGG GGCCGGTGCA ATAGGGCCGG ACGCCGTGTC CCAGGGGTAC 
CCGCCGGCCC CCCAAGGTGT GCTGGCCCCC CCCATGGGCG AGCTCTCCGG CCTGGAGGGC 
GTGCCCATGC CCCCCGCGGC CGGCGTGGCC CCGCCCGCGT ACCTGAGTGG GGGCGACATG 
GACGAGCGCT GCACTGTCTT GGCGCCCGGC GGAAGGGACC TGGACCTCAG GACAGGGGGT 
CTGTCCCCTC CCAGGGACAG AGATAGGCGG CGGCACAGTC GTTCCCGCTC TCCTCGGAGG 
CGTGGCCGTT CCCGGTCTCG GTCGCCCTCC CAGCGCACCA CGCCGGGTGC CCGGTACAAC 
GCCGCCAAGC GGGACCGGTC ACGCGAGCGG GAGAAGGAGC GGGAGCGGGA GAGGGACCGC 
CGGCGCAAGG GCCTGCCCCC CGTCAAGAAG GACTGCCTCA GCGTGTGCAG CTCGACCTTG 
TGGTTGGGAC ACGTGCCCAA GTCCGTGTCG GAGACGGACC TTCACGACAC CTTCGGCGAA 
TTTGGAAGCA TCGTCAAGAT TGAGATTATC GCCCCTCGTG GCTGTGCATT CGTGTGCATG 
GACCGGCGGC AGGATGCCTT TCGTGCCCTG CAGAAACTCG CAAAAGCACT CAAGCTTCAG 
GGGAGCCTCA TCAAGATGGC CTGGGCCCCT GGCAAGGGTG TGAAGAGCAA GGAGCTGAAG 
GACTTCTGGG AGGTTGACCT CGGCGTGAGC TACATCCCGT ACGAGAAGCT TCCCAAGGAC 
GTCGACCTCG TGTCCCTGGA AGAGGGCGGA GTCATCGACG AGGACTCTCT TCCTGAGGAC 
CTCAGAGAGA AGCGGAGGGC CCAGGAACAG CAGCGCACTC AGGGTGGGCC CGGTCTGGCA 
CCCTCCACGG CGGCCGGCGG CGGCGGTGCC GACGTGGTGG CCGCAATGGC AGCCTTTCCG 
GGGGCCGCCA TGACCACCCC AGTGGTGACC ACCATGGCTG CCCCCGGGGG TGCCGCCACT 
GCGGTGGCCA TGGTGCCGCC GCCCGTGGCA CAGTTTGGGA TCCCGCTGCC ACCAGTTGTC 
CAAGTTAAAC CGAGCGAACC TGAAATAAAA GGTGCATGCA TGCTGCCCTC GCAGGGCTTG 
GTGATGCCGC AGATACCAGT GCCCATGGGG GTGCCCGCCC CTCCAAACCA CATGCTGATG 
GTGCCACCCC ACGGCACCCA AACATTGCTG GGCCACAGTG CACTGGGACT CCTCGGTCCA 
CCGCCATTGG GAATCTCAGG AACTGCCCTG GGCCTTTCCC TGCACCCTCC CATGAGCCTC 
CCGACCACCT CCCAGTCTGC CCCTGTGCTG CCCGTGGCAA CAACAGCACA GCTGATGGCG 
GCGGCGGCAG CTGCGGCGGC CACGACGGCT ACGGTGACGA CCGACACCAC TGCGACCACT 
GCGACGCCCA TGGCCATCGA CGGGGATGCA ACGCCGACAG ACGAGGATGG AACGAGTGTC 
ATGGACAGCG TCAGCGCCAT TCCTACTCCG GCGGACCCTC CGGAACAACA AGGCGAGGCG 
TCCAAGGATT CAGTGTTTGG GAAACTTCTG CCACCACCAA TGTCTTTTCC GCCTCTCGCT 
GCGCCTCCAG GAGTTCCTTC GCAGTTCGTC ACTCAGGGAC TGGTGGGTGC TCAGAAGGAA 
CCGCACCCTG CCGAAGCTAA GTTCAAGTCT CCGGGATCTA GTCAAGAAAC TTCTAGTGGA 
AATGTGCAGG GCGCTGGCAT CTCTCAAGAG AAGCCACCAC CGGAAGGCAT GCAGATTGAG 
GACTCCAACT CCCAGGAACA GCAGAGCGAC TCAAGGACGG AGATACGCAG CTTCGAGACG 
TCACCCTCGG CGATACCTTT GCCGGTCGGA CTTCAGGGCT TTAACTCCTC GCCGATGCCT 
CCAAGAGGAC TGGGGGGACC TCCCATACGC TTCAGAGGAC CGCCCCTATT TCCGTTTGAT 
GGTCCACCGA TGCCCGATGG AAGACCTGGA ATGGACTTTC CAGGTCCGAG ACTGCTGGGG 
CCCGATCCTA GGATGATGGG ACCGCCTTCG GACTTCAGAC AGGGAAGCCC TCATGAGCAT 
CGAATGTTGG GTGGTCCCAT GGAGCAGCGG ATGATGGGTG GGGCAGAACA GCCGATGATG 
CGAGGTCCCA TGGAGCAAAG GCTGTTGGGC CCTCCAGAAC AGATGATGGG TGGGCCTCCG 
GACTCGCGGA TGTTGAGGGG TCCCCCAGAG CAAAGGTTTC TGGGCATGCC GCAGGAGCCA 
AGGATGCTGG GAGGTCCTTT GTCACCAAGA ATGCTAGGGG GTCCTCCGGA TGATAGACTA 
ATGGGTGGTC CACCAGATCA GAGGATGATG GGAGGACCAC GGGAGCACAG AATGTTGGGC 
GGTCCACCTC ACGAGCATAG AATGATGGGT GGTCCTCCTG AACACAGAAT GATGGGTGGG 
CCCCCAGACC ACCGGATGTT GGGTGGGCCC CTGGATCATA GGATGATGGG TGGGCCCCCT 
GACCACCGAA TGTTGGGTGG ACCGCTGGAC CACAGAATGA TGGGAGGCCC GCCAGACCAT 
AGGATGTTAA ACATTCCTCC GGAGCACAGG ATGATGGGCA GGCCAGGAGA TTTAGAACAC 
CGGATGATGG GAGGTCCCCC TGAACACCGG ATGAGGGGGG GTCCACCAGA CCATCGGATG 
ATGGGAGGTC CTCCGGATCA TAGGATGATG GGCGGGCCTC CCGACCACAG GATGATGGGC 
AGTCCACCTG AACACAGAAT GCTTCCAGGA CCTCAAGACC AGAGGATGAT GGGAGGTCCA 
CCGGACCACC GAATGCTCGG ACCTCCCGAT TTCATGATGG GTGGACCGCC GGAGTTTCGA 
AAGCTTGGAC CGCCGGACTC GCGAATGTTG GGACCGGGAC CTCGAATGCT GGGACCCAAT 
TTCGGCATGA TGAGCCCGAA TCAGATGGGG CCCCATGGTC CAGGCGGAGC AGACTCGGAC 
CCGGCAGATA AGATGGCAAA TTCTGATGGC AAGAAGGTGG CAACTGACAA AGAGGAAGGC 
AATGCCGGTG AAGGTGGTAA GGACGCGCAC TCGGGCGATA GGGAGGCAAA TATGGACGAA 
AAGAAAAACA GTCAAAACGA GCCAACCAAG AACTGGAATC ACCAGGTGGG AGGCGCCGAA 
GACGGCCGTG TACCAAATTT AATGGACGGT GGGCTGGGAA GACCCGACGG CGACAAGGGT 
CCCCAGAGCG ACGGCGATCG CGACAGGAAA GGCTTTGGCT TTTTTGGGCC ACCCCGTACG 
AGGTTTGACG GTCCAGAAGA GCGGTCCGGC TTCAGACCTG CCTTCAACGA TGGGCCAATG 
GATCGCGGTT CCGATAACGC CTGGGGAGGC AGGGACGGCG AAGACGGCGG TGGGTTTGGC 
GGCGATCGCC GCGTTTTCAC GAATGCCTTC GGCGAAATGT CAGAAAGGCG GCGGAGCGGC 
GGTGGTGGCG GCGGCGGCAG TGGGGGCGGC AGTGGCGGTC GAGGGGAGCG TAGGAGCCGC 
TGGTCTCGGG AAGACAACGA GTGGGACTCG CGGCGTAGTG ATCGCGCAGA TCGCAGTGAT 
CGAAGCGATC GTGACCGAAG TGATCGGGAT CGGAACGACC GTGACCGCAG AGACCGAGAC 
CGTACCGACG GCAAAGACAG AAGCGACCGG GATCGCTCGG ACAAGGACCG TAACGATCGG 
GAAAGAAGTG ATCGGGATCG GAGCGACCGG GATCGAAGCG ATCGTGACCG TAGTGATCGG 
GACCGGAGTG ACCGAGACCG CAGCGAGAAA GATCGGGACC GGAGTGAAAA GGACCGTAGC 
GACAGGGATC GCAGCGACAA AGATCGCAGT GAAAAGGATC GCAGCGACAA GGACCGCGGT 
GACAGGGATC GAGACCGAAG CAAAGACCGG GACCGAAGCA AAGATCGGGA CCGTAGCAAA 
GATCGGGACC GGAGCAAAGA CCGAGATCGA AGTAGAGACC GCGATCGGAG TGATAGGGAT 
CGAGATCGGA GTGATAGAGA AAAGGGCGAC AAGGATCGTG AGCGTAGCGA CAAGGATCGT 
GAGCGCAGTG ATAAAGACCG TGGTGATCGT GATCGGAGTG AGCGTGATCG CAGCAAAGGG 
GATTACCGGA GAAGCCGCGA GACTGACCGG GAGCGGGAAC GGAGCTCAGG CCGGAGCCAC 
AGCTCGTTAG AACAAAGCTC ATCGGCTGTC GAGACCAACG GAGAGGTCTC CAAGGCCAAC 
GGGAGCAGCT CACAGTCGTC GAAAGAGGAC AAGGCGCTAG GCAAGGCGCT AGACAGGGCG 
CTGGACAATG TGCTGGACAA GGCTGAGGCA CCCAAGCCCC AGGAAGTGGA GGAGGTCAGT 
GGGACGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8031660 ( XM_029983248.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029839108.1 Ixodes scapularis SR-related and CTD-associated factor 8 (LOC8031660), transcript variant X2, mRNA.

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CloneID	OIa25777
Clone ID Related Accession (Same CDS sequence)	XM_029983248.1
Accession Version	XM_029983248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	SR-related and CTD-associated factor 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021920090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTTG TTAAAGCCTT CAACGGCGAG CTTTCCTCGC TGTATGAAGG CAAGCCTCCC 
ATCTCGAAAG CCAAGATGAC AGCAATTACA AAGTCTGCCA TCAAGGGCAT CAAATTCTAC 
AAACACATAG TTCAGAGCGT GGAGAAGTTT GTTCAGAAGT GCCGGCCCGA GTACAAGGTT 
CCAGGCCTGT ACGTTGTGGA CTCGATTGTG CGCCAGTCGC GGCACCAGTT TGGCCAGGAG 
AAGGATGTGT TCGCACCCCG GTTCACCAAG AACATCCACA ACACCTTCCA GCACCTCTTC 
AAGTGCCCCG TGGAGGACAG ACCCAAGATT GTGCGGGTGC TCAATTTGTG GCAGAAGAAC 
CAGGTGTTCC CCCCGGATGT CATCCAGCCG CTTTTGGACA TGGCGAGCCC CAGTGCGGTG 
TTGGCGGCAG GGGACATGAG CGAGGGGCCC GACATGGGCC TGGACTCAAA GCCCCCCAGC 
AAGGCGGCGA TGGACCAGAT GGCCTCCTGG AAGGGCTGGA ACCTGGGAGG CAACAGCGTC 
CAGGACAATG CCTCCGCCCC TCCACAGGAG ACCAAGACCT ACGCCCTGAC GCAGGAGAAC 
TTGAACCTGG CCCTGGCGGC AGAGGCGGAA GGCGCGAGCG CCCCGGACCC CGAGATGCTG 
GTGAAGCTGC AGAGCCTGGC GTCGCAGCTG CTGGGGGCGA GAGCGCTGGG AAGCGGGAAC 
GCCATTGCCG AAGAGAAGCC CAGTCAGCCC TCGGCCCCGG CCGTCAAGTT CAACAAGAAA 
CTGCTGGACT TTGACTACGG GGACGAAGAG GAAGAGGAGG AGGAGGAACC CGTGGAGGAA 
ACAAGCACCC TGGCCCCGCC CGAGACCAAC CTGGAGGACT CCGGAGGAAG GCCGTCAGAA 
CCCAATGCTC TGGCACTCAG CATGGCTCAG AACCTGCTGA ACAATCCAGA GTTGCTGAAG 
CAGCTGCAGC AGATGCAGCA GACCCTTCAG CAGACGGAGG TGCTTAGGGG CTCGGAGACG 
TCGGCCGCCC CCGCAGCCCC TTCGTCGCTC GGAACGGCCG CGCTCCCCCA GTGCTCGGAG 
ACTGGCAAGG CGCCCTCCCC TCATCCGCAG ACCCCGCAGG GGCCGGACGG GCAGCCGCTG 
GCGACGACTG CGGGGTACCT GACGGCCTCG GCCTCGGTGG TGAACTCCCT GGCGCCTGCG 
GTGAGCTCTG GGCTTTCCCA GCTTTTCCAG ACAGACTTTG CGGGGGCCTC TGGTGCTCCT 
TTGCTGCCTG AATCTCTGAG CCAGCTGAAC ATGCCCCTTT ACCCGCCGGC GATGGCGATG 
CCTCAGGGGG TCCCCTATGG GGCCGGTGCA ATAGGGCCGG ACGCCGTGTC CCAGGGGTAC 
CCGCCGGCCC CCCAAGGTGT GCTGGCCCCC CCCATGGGCG AGCTCTCCGG CCTGGAGGGC 
GTGCCCATGC CCCCCGCGGC CGGCGTGGCC CCGCCCGCGT ACCTGAGTGG GGGCGACATG 
GACGAGCGCT GCACTGTCTT GGCGCCCGGC GGAAGGGACC TGGACCTCAG GACAGGGGGT 
CTGTCCCCTC CCAGGGACAG AGATAGGCGG CGGCACAGTC GTTCCCGCTC TCCTCGGAGG 
CGTGGCCGTT CCCGGTCTCG GTCGCCCTCC CAGCGCACCA CGCCGGGTGC CCGGTACAAC 
GCCGCCAAGC GGGACCGGTC ACGCGAGCGG GAGAAGGAGC GGGAGCGGGA GAGGGACCGC 
CGGCGCAAGG GCCTGCCCCC CGTCAAGAAG GACTGCCTCA GCGTGTGCAG CTCGACCTTG 
TGGTTGGGAC ACGTGCCCAA GTCCGTGTCG GAGACGGACC TTCACGACAC CTTCGGCGAA 
TTTGGAAGCA TCGTCAAGAT TGAGATTATC GCCCCTCGTG GCTGTGCATT CGTGTGCATG 
GACCGGCGGC AGGATGCCTT TCGTGCCCTG CAGAAACTCG CAAAAGCACT CAAGCTTCAG 
GGGAGCCTCA TCAAGATGGC CTGGGCCCCT GGCAAGGGTG TGAAGAGCAA GGAGCTGAAG 
GACTTCTGGG AGGTTGACCT CGGCGTGAGC TACATCCCGT ACGAGAAGCT TCCCAAGGAC 
GTCGACCTCG TGTCCCTGGA AGAGGGCGGA GTCATCGACG AGGACTCTCT TCCTGAGGAC 
CTCAGAGAGA AGCGGAGGGC CCAGGAACAG CAGCGCACTC AGGGTGGGCC CGGTCTGGCA 
CCCTCCACGG CGGCCGGCGG CGGCGGTGCC GACGTGGTGG CCGCAATGGC AGCCTTTCCG 
GGGGCCGCCA TGACCACCCC AGTGGTGACC ACCATGGCTG CCCCCGGGGG TGCCGCCACT 
GCGGTGGCCA TGGTGCCGCC GCCCGTGGCA CAGTTTGGGA TCCCGCTGCC ACCAGTTGTC 
CAAGTTAAAC CGAGCGAACC TGAAATAAAA GGCATGCTGC CCTCGCAGGG CTTGGTGATG 
CCGCAGATAC CAGTGCCCAT GGGGGTGCCC GCCCCTCCAA ACCACATGCT GATGGTGCCA 
CCCCACGGCA CCCAAACATT GCTGGGCCAC AGTGCACTGG GACTCCTCGG TCCACCGCCA 
TTGGGAATCT CAGGAACTGC CCTGGGCCTT TCCCTGCACC CTCCCATGAG CCTCCCGACC 
ACCTCCCAGT CTGCCCCTGT GCTGCCCGTG GCAACAACAG CACAGCTGAT GGCGGCGGCG 
GCAGCTGCGG CGGCCACGAC GGCTACGGTG ACGACCGACA CCACTGCGAC CACTGCGACG 
CCCATGGCCA TCGACGGGGA TGCAACGCCG ACAGACGAGG ATGGAACGAG TGTCATGGAC 
AGCGTCAGCG CCATTCCTAC TCCGGCGGAC CCTCCGGAAC AACAAGGCGA GGCGTCCAAG 
GATTCAGTGT TTGGGAAACT TCTGCCACCA CCAATGTCTT TTCCGCCTCT CGCTGCGCCT 
CCAGGAGTTC CTTCGCAGTT CGTCACTCAG GGACTGGTGG GTGCTCAGAA GGAACCGCAC 
CCTGCCGAAG CTAAGTTCAA GTCTCCGGGA TCTAGTCAAG AAACTTCTAG TGGAAATGTG 
CAGGGCGCTG GCATCTCTCA AGAGAAGCCA CCACCGGAAG GCATGCAGAT TGAGGACTCC 
AACTCCCAGG AACAGCAGAG CGACTCAAGG ACGGAGATAC GCAGCTTCGA GACGTCACCC 
TCGGCGATAC CTTTGCCGGT CGGACTTCAG GGCTTTAACT CCTCGCCGAT GCCTCCAAGA 
GGACTGGGGG GACCTCCCAT ACGCTTCAGA GGACCGCCCC TATTTCCGTT TGATGGTCCA 
CCGATGCCCG ATGGAAGACC TGGAATGGAC TTTCCAGGTC CGAGACTGCT GGGGCCCGAT 
CCTAGGATGA TGGGACCGCC TTCGGACTTC AGACAGGGAA GCCCTCATGA GCATCGAATG 
TTGGGTGGTC CCATGGAGCA GCGGATGATG GGTGGGGCAG AACAGCCGAT GATGCGAGGT 
CCCATGGAGC AAAGGCTGTT GGGCCCTCCA GAACAGATGA TGGGTGGGCC TCCGGACTCG 
CGGATGTTGA GGGGTCCCCC AGAGCAAAGG TTTCTGGGCA TGCCGCAGGA GCCAAGGATG 
CTGGGAGGTC CTTTGTCACC AAGAATGCTA GGGGGTCCTC CGGATGATAG ACTAATGGGT 
GGTCCACCAG ATCAGAGGAT GATGGGAGGA CCACGGGAGC ACAGAATGTT GGGCGGTCCA 
CCTCACGAGC ATAGAATGAT GGGTGGTCCT CCTGAACACA GAATGATGGG TGGGCCCCCA 
GACCACCGGA TGTTGGGTGG GCCCCTGGAT CATAGGATGA TGGGTGGGCC CCCTGACCAC 
CGAATGTTGG GTGGACCGCT GGACCACAGA ATGATGGGAG GCCCGCCAGA CCATAGGATG 
TTAAACATTC CTCCGGAGCA CAGGATGATG GGCAGGCCAG GAGATTTAGA ACACCGGATG 
ATGGGAGGTC CCCCTGAACA CCGGATGAGG GGGGGTCCAC CAGACCATCG GATGATGGGA 
GGTCCTCCGG ATCATAGGAT GATGGGCGGG CCTCCCGACC ACAGGATGAT GGGCAGTCCA 
CCTGAACACA GAATGCTTCC AGGACCTCAA GACCAGAGGA TGATGGGAGG TCCACCGGAC 
CACCGAATGC TCGGACCTCC CGATTTCATG ATGGGTGGAC CGCCGGAGTT TCGAAAGCTT 
GGACCGCCGG ACTCGCGAAT GTTGGGACCG GGACCTCGAA TGCTGGGACC CAATTTCGGC 
ATGATGAGCC CGAATCAGAT GGGGCCCCAT GGTCCAGGCG GAGCAGACTC GGACCCGGCA 
GATAAGATGG CAAATTCTGA TGGCAAGAAG GTGGCAACTG ACAAAGAGGA AGGCAATGCC 
GGTGAAGGTG GTAAGGACGC GCACTCGGGC GATAGGGAGG CAAATATGGA CGAAAAGAAA 
AACAGTCAAA ACGAGCCAAC CAAGAACTGG AATCACCAGG TGGGAGGCGC CGAAGACGGC 
CGTGTACCAA ATTTAATGGA CGGTGGGCTG GGAAGACCCG ACGGCGACAA GGGTCCCCAG 
AGCGACGGCG ATCGCGACAG GAAAGGCTTT GGCTTTTTTG GGCCACCCCG TACGAGGTTT 
GACGGTCCAG AAGAGCGGTC CGGCTTCAGA CCTGCCTTCA ACGATGGGCC AATGGATCGC 
GGTTCCGATA ACGCCTGGGG AGGCAGGGAC GGCGAAGACG GCGGTGGGTT TGGCGGCGAT 
CGCCGCGTTT TCACGAATGC CTTCGGCGAA ATGTCAGAAA GGCGGCGGAG CGGCGGTGGT 
GGCGGCGGCG GCAGTGGGGG CGGCAGTGGC GGTCGAGGGG AGCGTAGGAG CCGCTGGTCT 
CGGGAAGACA ACGAGTGGGA CTCGCGGCGT AGTGATCGCG CAGATCGCAG TGATCGAAGC 
GATCGTGACC GAAGTGATCG GGATCGGAAC GACCGTGACC GCAGAGACCG AGACCGTACC 
GACGGCAAAG ACAGAAGCGA CCGGGATCGC TCGGACAAGG ACCGTAACGA TCGGGAAAGA 
AGTGATCGGG ATCGGAGCGA CCGGGATCGA AGCGATCGTG ACCGTAGTGA TCGGGACCGG 
AGTGACCGAG ACCGCAGCGA GAAAGATCGG GACCGGAGTG AAAAGGACCG TAGCGACAGG 
GATCGCAGCG ACAAAGATCG CAGTGAAAAG GATCGCAGCG ACAAGGACCG CGGTGACAGG 
GATCGAGACC GAAGCAAAGA CCGGGACCGA AGCAAAGATC GGGACCGTAG CAAAGATCGG 
GACCGGAGCA AAGACCGAGA TCGAAGTAGA GACCGCGATC GGAGTGATAG GGATCGAGAT 
CGGAGTGATA GAGAAAAGGG CGACAAGGAT CGTGAGCGTA GCGACAAGGA TCGTGAGCGC 
AGTGATAAAG ACCGTGGTGA TCGTGATCGG AGTGAGCGTG ATCGCAGCAA AGGGGATTAC 
CGGAGAAGCC GCGAGACTGA CCGGGAGCGG GAACGGAGCT CAGGCCGGAG CCACAGCTCG 
TTAGAACAAA GCTCATCGGC TGTCGAGACC AACGGAGAGG TCTCCAAGGC CAACGGGAGC 
AGCTCACAGT CGTCGAAAGA GGACAAGGCG CTAGGCAAGG CGCTAGACAG GGCGCTGGAC 
AATGTGCTGG ACAAGGCTGA GGCACCCAAG CCCCAGGAAG TGGAGGAGGT CAGTGGGACG 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029839108.1
CDS	93..5798
Translation	MEVVKAFNGELSSLYEGKPPISKAKMTAITKSAIKGIKFYKHIVQSVEKFVQKCRPEYKVPGLYVVDSIVRQSRHQFGQEKDVFAPRFTKNIHNTFQHLFKCPVEDRPKIVRVLNLWQKNQVFPPDVIQPLLDMASPSAVLAAGDMSEGPDMGLDSKPPSKAAMDQMASWKGWNLGGNSVQDNASAPPQETKTYALTQENLNLALAAEAEGASAPDPEMLVKLQSLASQLLGARALGSGNAIAEEKPSQPSAPAVKFNKKLLDFDYGDEEEEEEEEPVEETSTLAPPETNLEDSGGRPSEPNALALSMAQNLLNNPELLKQLQQMQQTLQQTEVLRGSETSAAPAAPSSLGTAALPQCSETGKAPSPHPQTPQGPDGQPLATTAGYLTASASVVNSLAPAVSSGLSQLFQTDFAGASGAPLLPESLSQLNMPLYPPAMAMPQGVPYGAGAIGPDAVSQGYPPAPQGVLAPPMGELSGLEGVPMPPAAGVAPPAYLSGGDMDERCTVLAPGGRDLDLRTGGLSPPRDRDRRRHSRSRSPRRRGRSRSRSPSQRTTPGARYNAAKRDRSREREKERERERDRRRKGLPPVKKDCLSVCSSTLWLGHVPKSVSETDLHDTFGEFGSIVKIEIIAPRGCAFVCMDRRQDAFRALQKLAKALKLQGSLIKMAWAPGKGVKSKELKDFWEVDLGVSYIPYEKLPKDVDLVSLEEGGVIDEDSLPEDLREKRRAQEQQRTQGGPGLAPSTAAGGGGADVVAAMAAFPGAAMTTPVVTTMAAPGGAATAVAMVPPPVAQFGIPLPPVVQVKPSEPEIKGMLPSQGLVMPQIPVPMGVPAPPNHMLMVPPHGTQTLLGHSALGLLGPPPLGISGTALGLSLHPPMSLPTTSQSAPVLPVATTAQLMAAAAAAAATTATVTTDTTATTATPMAIDGDATPTDEDGTSVMDSVSAIPTPADPPEQQGEASKDSVFGKLLPPPMSFPPLAAPPGVPSQFVTQGLVGAQKEPHPAEAKFKSPGSSQETSSGNVQGAGISQEKPPPEGMQIEDSNSQEQQSDSRTEIRSFETSPSAIPLPVGLQGFNSSPMPPRGLGGPPIRFRGPPLFPFDGPPMPDGRPGMDFPGPRLLGPDPRMMGPPSDFRQGSPHEHRMLGGPMEQRMMGGAEQPMMRGPMEQRLLGPPEQMMGGPPDSRMLRGPPEQRFLGMPQEPRMLGGPLSPRMLGGPPDDRLMGGPPDQRMMGGPREHRMLGGPPHEHRMMGGPPEHRMMGGPPDHRMLGGPLDHRMMGGPPDHRMLGGPLDHRMMGGPPDHRMLNIPPEHRMMGRPGDLEHRMMGGPPEHRMRGGPPDHRMMGGPPDHRMMGGPPDHRMMGSPPEHRMLPGPQDQRMMGGPPDHRMLGPPDFMMGGPPEFRKLGPPDSRMLGPGPRMLGPNFGMMSPNQMGPHGPGGADSDPADKMANSDGKKVATDKEEGNAGEGGKDAHSGDREANMDEKKNSQNEPTKNWNHQVGGAEDGRVPNLMDGGLGRPDGDKGPQSDGDRDRKGFGFFGPPRTRFDGPEERSGFRPAFNDGPMDRGSDNAWGGRDGEDGGGFGGDRRVFTNAFGEMSERRRSGGGGGGGSGGGSGGRGERRSRWSREDNEWDSRRSDRADRSDRSDRDRSDRDRNDRDRRDRDRTDGKDRSDRDRSDKDRNDRERSDRDRSDRDRSDRDRSDRDRSDRDRSEKDRDRSEKDRSDRDRSDKDRSEKDRSDKDRGDRDRDRSKDRDRSKDRDRSKDRDRSKDRDRSRDRDRSDRDRDRSDREKGDKDRERSDKDRERSDKDRGDRDRSERDRSKGDYRRSRETDRERERSSGRSHSSLEQSSSAVETNGEVSKANGSSSQSSKEDKALGKALDRALDNVLDKAEAPKPQEVEEVSGTA

Target ORF information:

RefSeq Version	XM_029983248.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis SR-related and CTD-associated factor 8 (LOC8031660), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029983248.1

ORF Insert Sequence:

ATGGAGGTTG TTAAAGCCTT CAACGGCGAG CTTTCCTCGC TGTATGAAGG CAAGCCTCCC 
ATCTCGAAAG CCAAGATGAC AGCAATTACA AAGTCTGCCA TCAAGGGCAT CAAATTCTAC 
AAACACATAG TTCAGAGCGT GGAGAAGTTT GTTCAGAAGT GCCGGCCCGA GTACAAGGTT 
CCAGGCCTGT ACGTTGTGGA CTCGATTGTG CGCCAGTCGC GGCACCAGTT TGGCCAGGAG 
AAGGATGTGT TCGCACCCCG GTTCACCAAG AACATCCACA ACACCTTCCA GCACCTCTTC 
AAGTGCCCCG TGGAGGACAG ACCCAAGATT GTGCGGGTGC TCAATTTGTG GCAGAAGAAC 
CAGGTGTTCC CCCCGGATGT CATCCAGCCG CTTTTGGACA TGGCGAGCCC CAGTGCGGTG 
TTGGCGGCAG GGGACATGAG CGAGGGGCCC GACATGGGCC TGGACTCAAA GCCCCCCAGC 
AAGGCGGCGA TGGACCAGAT GGCCTCCTGG AAGGGCTGGA ACCTGGGAGG CAACAGCGTC 
CAGGACAATG CCTCCGCCCC TCCACAGGAG ACCAAGACCT ACGCCCTGAC GCAGGAGAAC 
TTGAACCTGG CCCTGGCGGC AGAGGCGGAA GGCGCGAGCG CCCCGGACCC CGAGATGCTG 
GTGAAGCTGC AGAGCCTGGC GTCGCAGCTG CTGGGGGCGA GAGCGCTGGG AAGCGGGAAC 
GCCATTGCCG AAGAGAAGCC CAGTCAGCCC TCGGCCCCGG CCGTCAAGTT CAACAAGAAA 
CTGCTGGACT TTGACTACGG GGACGAAGAG GAAGAGGAGG AGGAGGAACC CGTGGAGGAA 
ACAAGCACCC TGGCCCCGCC CGAGACCAAC CTGGAGGACT CCGGAGGAAG GCCGTCAGAA 
CCCAATGCTC TGGCACTCAG CATGGCTCAG AACCTGCTGA ACAATCCAGA GTTGCTGAAG 
CAGCTGCAGC AGATGCAGCA GACCCTTCAG CAGACGGAGG TGCTTAGGGG CTCGGAGACG 
TCGGCCGCCC CCGCAGCCCC TTCGTCGCTC GGAACGGCCG CGCTCCCCCA GTGCTCGGAG 
ACTGGCAAGG CGCCCTCCCC TCATCCGCAG ACCCCGCAGG GGCCGGACGG GCAGCCGCTG 
GCGACGACTG CGGGGTACCT GACGGCCTCG GCCTCGGTGG TGAACTCCCT GGCGCCTGCG 
GTGAGCTCTG GGCTTTCCCA GCTTTTCCAG ACAGACTTTG CGGGGGCCTC TGGTGCTCCT 
TTGCTGCCTG AATCTCTGAG CCAGCTGAAC ATGCCCCTTT ACCCGCCGGC GATGGCGATG 
CCTCAGGGGG TCCCCTATGG GGCCGGTGCA ATAGGGCCGG ACGCCGTGTC CCAGGGGTAC 
CCGCCGGCCC CCCAAGGTGT GCTGGCCCCC CCCATGGGCG AGCTCTCCGG CCTGGAGGGC 
GTGCCCATGC CCCCCGCGGC CGGCGTGGCC CCGCCCGCGT ACCTGAGTGG GGGCGACATG 
GACGAGCGCT GCACTGTCTT GGCGCCCGGC GGAAGGGACC TGGACCTCAG GACAGGGGGT 
CTGTCCCCTC CCAGGGACAG AGATAGGCGG CGGCACAGTC GTTCCCGCTC TCCTCGGAGG 
CGTGGCCGTT CCCGGTCTCG GTCGCCCTCC CAGCGCACCA CGCCGGGTGC CCGGTACAAC 
GCCGCCAAGC GGGACCGGTC ACGCGAGCGG GAGAAGGAGC GGGAGCGGGA GAGGGACCGC 
CGGCGCAAGG GCCTGCCCCC CGTCAAGAAG GACTGCCTCA GCGTGTGCAG CTCGACCTTG 
TGGTTGGGAC ACGTGCCCAA GTCCGTGTCG GAGACGGACC TTCACGACAC CTTCGGCGAA 
TTTGGAAGCA TCGTCAAGAT TGAGATTATC GCCCCTCGTG GCTGTGCATT CGTGTGCATG 
GACCGGCGGC AGGATGCCTT TCGTGCCCTG CAGAAACTCG CAAAAGCACT CAAGCTTCAG 
GGGAGCCTCA TCAAGATGGC CTGGGCCCCT GGCAAGGGTG TGAAGAGCAA GGAGCTGAAG 
GACTTCTGGG AGGTTGACCT CGGCGTGAGC TACATCCCGT ACGAGAAGCT TCCCAAGGAC 
GTCGACCTCG TGTCCCTGGA AGAGGGCGGA GTCATCGACG AGGACTCTCT TCCTGAGGAC 
CTCAGAGAGA AGCGGAGGGC CCAGGAACAG CAGCGCACTC AGGGTGGGCC CGGTCTGGCA 
CCCTCCACGG CGGCCGGCGG CGGCGGTGCC GACGTGGTGG CCGCAATGGC AGCCTTTCCG 
GGGGCCGCCA TGACCACCCC AGTGGTGACC ACCATGGCTG CCCCCGGGGG TGCCGCCACT 
GCGGTGGCCA TGGTGCCGCC GCCCGTGGCA CAGTTTGGGA TCCCGCTGCC ACCAGTTGTC 
CAAGTTAAAC CGAGCGAACC TGAAATAAAA GGCATGCTGC CCTCGCAGGG CTTGGTGATG 
CCGCAGATAC CAGTGCCCAT GGGGGTGCCC GCCCCTCCAA ACCACATGCT GATGGTGCCA 
CCCCACGGCA CCCAAACATT GCTGGGCCAC AGTGCACTGG GACTCCTCGG TCCACCGCCA 
TTGGGAATCT CAGGAACTGC CCTGGGCCTT TCCCTGCACC CTCCCATGAG CCTCCCGACC 
ACCTCCCAGT CTGCCCCTGT GCTGCCCGTG GCAACAACAG CACAGCTGAT GGCGGCGGCG 
GCAGCTGCGG CGGCCACGAC GGCTACGGTG ACGACCGACA CCACTGCGAC CACTGCGACG 
CCCATGGCCA TCGACGGGGA TGCAACGCCG ACAGACGAGG ATGGAACGAG TGTCATGGAC 
AGCGTCAGCG CCATTCCTAC TCCGGCGGAC CCTCCGGAAC AACAAGGCGA GGCGTCCAAG 
GATTCAGTGT TTGGGAAACT TCTGCCACCA CCAATGTCTT TTCCGCCTCT CGCTGCGCCT 
CCAGGAGTTC CTTCGCAGTT CGTCACTCAG GGACTGGTGG GTGCTCAGAA GGAACCGCAC 
CCTGCCGAAG CTAAGTTCAA GTCTCCGGGA TCTAGTCAAG AAACTTCTAG TGGAAATGTG 
CAGGGCGCTG GCATCTCTCA AGAGAAGCCA CCACCGGAAG GCATGCAGAT TGAGGACTCC 
AACTCCCAGG AACAGCAGAG CGACTCAAGG ACGGAGATAC GCAGCTTCGA GACGTCACCC 
TCGGCGATAC CTTTGCCGGT CGGACTTCAG GGCTTTAACT CCTCGCCGAT GCCTCCAAGA 
GGACTGGGGG GACCTCCCAT ACGCTTCAGA GGACCGCCCC TATTTCCGTT TGATGGTCCA 
CCGATGCCCG ATGGAAGACC TGGAATGGAC TTTCCAGGTC CGAGACTGCT GGGGCCCGAT 
CCTAGGATGA TGGGACCGCC TTCGGACTTC AGACAGGGAA GCCCTCATGA GCATCGAATG 
TTGGGTGGTC CCATGGAGCA GCGGATGATG GGTGGGGCAG AACAGCCGAT GATGCGAGGT 
CCCATGGAGC AAAGGCTGTT GGGCCCTCCA GAACAGATGA TGGGTGGGCC TCCGGACTCG 
CGGATGTTGA GGGGTCCCCC AGAGCAAAGG TTTCTGGGCA TGCCGCAGGA GCCAAGGATG 
CTGGGAGGTC CTTTGTCACC AAGAATGCTA GGGGGTCCTC CGGATGATAG ACTAATGGGT 
GGTCCACCAG ATCAGAGGAT GATGGGAGGA CCACGGGAGC ACAGAATGTT GGGCGGTCCA 
CCTCACGAGC ATAGAATGAT GGGTGGTCCT CCTGAACACA GAATGATGGG TGGGCCCCCA 
GACCACCGGA TGTTGGGTGG GCCCCTGGAT CATAGGATGA TGGGTGGGCC CCCTGACCAC 
CGAATGTTGG GTGGACCGCT GGACCACAGA ATGATGGGAG GCCCGCCAGA CCATAGGATG 
TTAAACATTC CTCCGGAGCA CAGGATGATG GGCAGGCCAG GAGATTTAGA ACACCGGATG 
ATGGGAGGTC CCCCTGAACA CCGGATGAGG GGGGGTCCAC CAGACCATCG GATGATGGGA 
GGTCCTCCGG ATCATAGGAT GATGGGCGGG CCTCCCGACC ACAGGATGAT GGGCAGTCCA 
CCTGAACACA GAATGCTTCC AGGACCTCAA GACCAGAGGA TGATGGGAGG TCCACCGGAC 
CACCGAATGC TCGGACCTCC CGATTTCATG ATGGGTGGAC CGCCGGAGTT TCGAAAGCTT 
GGACCGCCGG ACTCGCGAAT GTTGGGACCG GGACCTCGAA TGCTGGGACC CAATTTCGGC 
ATGATGAGCC CGAATCAGAT GGGGCCCCAT GGTCCAGGCG GAGCAGACTC GGACCCGGCA 
GATAAGATGG CAAATTCTGA TGGCAAGAAG GTGGCAACTG ACAAAGAGGA AGGCAATGCC 
GGTGAAGGTG GTAAGGACGC GCACTCGGGC GATAGGGAGG CAAATATGGA CGAAAAGAAA 
AACAGTCAAA ACGAGCCAAC CAAGAACTGG AATCACCAGG TGGGAGGCGC CGAAGACGGC 
CGTGTACCAA ATTTAATGGA CGGTGGGCTG GGAAGACCCG ACGGCGACAA GGGTCCCCAG 
AGCGACGGCG ATCGCGACAG GAAAGGCTTT GGCTTTTTTG GGCCACCCCG TACGAGGTTT 
GACGGTCCAG AAGAGCGGTC CGGCTTCAGA CCTGCCTTCA ACGATGGGCC AATGGATCGC 
GGTTCCGATA ACGCCTGGGG AGGCAGGGAC GGCGAAGACG GCGGTGGGTT TGGCGGCGAT 
CGCCGCGTTT TCACGAATGC CTTCGGCGAA ATGTCAGAAA GGCGGCGGAG CGGCGGTGGT 
GGCGGCGGCG GCAGTGGGGG CGGCAGTGGC GGTCGAGGGG AGCGTAGGAG CCGCTGGTCT 
CGGGAAGACA ACGAGTGGGA CTCGCGGCGT AGTGATCGCG CAGATCGCAG TGATCGAAGC 
GATCGTGACC GAAGTGATCG GGATCGGAAC GACCGTGACC GCAGAGACCG AGACCGTACC 
GACGGCAAAG ACAGAAGCGA CCGGGATCGC TCGGACAAGG ACCGTAACGA TCGGGAAAGA 
AGTGATCGGG ATCGGAGCGA CCGGGATCGA AGCGATCGTG ACCGTAGTGA TCGGGACCGG 
AGTGACCGAG ACCGCAGCGA GAAAGATCGG GACCGGAGTG AAAAGGACCG TAGCGACAGG 
GATCGCAGCG ACAAAGATCG CAGTGAAAAG GATCGCAGCG ACAAGGACCG CGGTGACAGG 
GATCGAGACC GAAGCAAAGA CCGGGACCGA AGCAAAGATC GGGACCGTAG CAAAGATCGG 
GACCGGAGCA AAGACCGAGA TCGAAGTAGA GACCGCGATC GGAGTGATAG GGATCGAGAT 
CGGAGTGATA GAGAAAAGGG CGACAAGGAT CGTGAGCGTA GCGACAAGGA TCGTGAGCGC 
AGTGATAAAG ACCGTGGTGA TCGTGATCGG AGTGAGCGTG ATCGCAGCAA AGGGGATTAC 
CGGAGAAGCC GCGAGACTGA CCGGGAGCGG GAACGGAGCT CAGGCCGGAG CCACAGCTCG 
TTAGAACAAA GCTCATCGGC TGTCGAGACC AACGGAGAGG TCTCCAAGGC CAACGGGAGC 
AGCTCACAGT CGTCGAAAGA GGACAAGGCG CTAGGCAAGG CGCTAGACAG GGCGCTGGAC 
AATGTGCTGG ACAAGGCTGA GGCACCCAAG CCCCAGGAAG TGGAGGAGGT CAGTGGGACG 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8031660 ( XM_029983249.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029839109.1 Ixodes scapularis SR-related and CTD-associated factor 8 (LOC8031660), transcript variant X3, mRNA.

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CloneID	OIa25778
Clone ID Related Accession (Same CDS sequence)	XM_029983249.1
Accession Version	XM_029983249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	SR-related and CTD-associated factor 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021920090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTTG TTAAAGCCTT CAACGGCGAG CTTTCCTCGC TGTATGAAGG CAAGCCTCCC 
ATCTCGAAAG CCAAGATGAC AGCAATTACA AAGTCTGCCA TCAAGGGCAT CAAATTCTAC 
AAACACATAG TTCAGAGCGT GGAGAAGTTT GTTCAGAAGT GCCGGCCCGA GTACAAGGTT 
CCAGGCCTGT ACGTTGTGGA CTCGATTGTG CGCCAGTCGC GGCACCAGTT TGGCCAGGAG 
AAGGATGTGT TCGCACCCCG GTTCACCAAG AACATCCACA ACACCTTCCA GCACCTCTTC 
AAGTGCCCCG TGGAGGACAG ACCCAAGATT GTGCGGGTGC TCAATTTGTG GCAGAAGAAC 
CAGGTGTTCC CCCCGGATGT CATCCAGCCG CTTTTGGACA TGGCGAGCCC CAGTGCGGTG 
TTGGCGGCAG GGGACATGAG CGAGGGGCCC GACATGGGCC TGGACTCAAA GCCCCCCAGC 
AAGGCGGCGA TGGACCAGAT GGCCTCCTGG AAGGGCTGGA ACCTGGGAGG CAACAGCGTC 
CAGGACAATG CCTCCGCCCC TCCACAGGAG ACCAAGACCT ACGCCCTGAC GCAGGAGAAC 
TTGAACCTGG CCCTGGCGGC AGAGGCGGAA GGCGCGAGCG CCCCGGACCC CGAGATGCTG 
GTGAAGCTGC AGAGCCTGGC GTCGCAGCTG CTGGGGGCGA GAGCGCTGGG AAGCGGGAAC 
GCCATTGCCG AAGAGAAGCC CAGTCAGCCC TCGGCCCCGG CCGTCAAGTT CAACAAGAAA 
CTGCTGGACT TTGACTACGG GGACGAAGAG GAAGAGGAGG AGGAGGAACC CGTGGAGGAA 
ACAAGCACCC TGGCCCCGCC CGAGACCAAC CTGGAGGACT CCGGAGGAAG GCCGTCAGAA 
CCCAATGCTC TGGCACTCAG CATGGCTCAG AACCTGCTGA ACAATCCAGA GTTGCTGAAG 
CAGCTGCAGC AGATGCAGCA GACCCTTCAG CAGACGGAGG TGCTTAGGGG CTCGGAGACG 
TCGGCCGCCC CCGCAGCCCC TTCGTCGCTC GGAACGGCCG CGCTCCCCCA GTGCTCGGAG 
ACTGGCAAGG CGCCCTCCCC TCATCCGCAG ACCCCGCAGG GGCCGGACGG GCAGCCGCTG 
GCGACGACTG CGGGGTACCT GACGGCCTCG GCCTCGGTGG TGAACTCCCT GGCGCCTGCG 
GTGAGCTCTG GGCTTTCCCA GCTTTTCCAG ACAGACTTTG CGGGGGCCTC TGGTGCTCCT 
TTGCTGCCTG AATCTCTGAG CCAGCTGAAC ATGCCCCTTT ACCCGCCGGC GATGGCGATG 
CCTCAGGGGG TCCCCTATGG GGCCGGTGCA ATAGGGCCGG ACGCCGTGTC CCAGGGGTAC 
CCGCCGGCCC CCCAAGGTGT GCTGGCCCCC CCCATGGGCG AGCTCTCCGG CCTGGAGGGC 
GTGCCCATGC CCCCCGCGGC CGGCGTGGCC CCGCCCGCGT ACCTGAGTGG GGGCGACATG 
GACGAGCGCT GCACTGTCTT GGCGCCCGGC GGAAGGGACC TGGACCTCAG GACAGGGGGT 
CTGTCCCCTC CCAGGGACAG AGATAGGCGG CGGCACAGTC GTTCCCGCTC TCCTCGGAGG 
CGTGGCCGTT CCCGGTCTCG GTCGCCCTCC CAGCGCACCA CGCCGGGTGC CCGGTACAAC 
GCCGCCAAGC GGGACCGGTC ACGCGAGCGG GAGAAGGAGC GGGAGCGGGA GAGGGACCGC 
CGGCGCAAGG GCCTGCCCCC CGTCAAGAAG GACTGCCTCA GCGTGTGCAG CTCGACCTTG 
TGGTTGGGAC ACGTGCCCAA GTCCGTGTCG GAGACGGACC TTCACGACAC CTTCGGCGAA 
TTTGGAAGCA TCGTCAAGAT TGAGATTATC GCCCCTCGTG GCTGTGCATT CGTGTGCATG 
GACCGGCGGC AGGATGCCTT TCGTGCCCTG CAGAAACTCG CAAAAGCACT CAAGCTTCAG 
GGGAGCCTCA TCAAGATGGC CTGGGCCCCT GGCAAGGGTG TGAAGAGCAA GGAGCTGAAG 
GACTTCTGGG AGGTTGACCT CGGCGTGAGC TACATCCCGT ACGAGAAGCT TCCCAAGGAC 
GTCGACCTCG TGTCCCTGGA AGAGGGCGGA GTCATCGACG AGGACTCTCT TCCTGAGGAC 
CTCAGAGAGA AGCGGAGGGC CCAGGAACAG CAGCGCACTC AGGGTGGGCC CGGTCTGGCA 
CCCTCCACGG CGGCCGGCGG CGGCGGTGCC GACGTGGTGG CCGCAATGGC AGCCTTTCCG 
GGGGCCGCCA TGACCACCCC AGTGGTGACC ACCATGGCTG CCCCCGGGGG TGCCGCCACT 
GCGGTGGCCA TGGTGCCGCC GCCCGTGGCA CAGTTTGGGA TCCCGCTGCC ACCAGGCATG 
CTGCCCTCGC AGGGCTTGGT GATGCCGCAG ATACCAGTGC CCATGGGGGT GCCCGCCCCT 
CCAAACCACA TGCTGATGGT GCCACCCCAC GGCACCCAAA CATTGCTGGG CCACAGTGCA 
CTGGGACTCC TCGGTCCACC GCCATTGGGA ATCTCAGGAA CTGCCCTGGG CCTTTCCCTG 
CACCCTCCCA TGAGCCTCCC GACCACCTCC CAGTCTGCCC CTGTGCTGCC CGTGGCAACA 
ACAGCACAGC TGATGGCGGC GGCGGCAGCT GCGGCGGCCA CGACGGCTAC GGTGACGACC 
GACACCACTG CGACCACTGC GACGCCCATG GCCATCGACG GGGATGCAAC GCCGACAGAC 
GAGGATGGAA CGAGTGTCAT GGACAGCGTC AGCGCCATTC CTACTCCGGC GGACCCTCCG 
GAACAACAAG GCGAGGCGTC CAAGGATTCA GTGTTTGGGA AACTTCTGCC ACCACCAATG 
TCTTTTCCGC CTCTCGCTGC GCCTCCAGGA GTTCCTTCGC AGTTCGTCAC TCAGGGACTG 
GTGGGTGCTC AGAAGGAACC GCACCCTGCC GAAGCTAAGT TCAAGTCTCC GGGATCTAGT 
CAAGAAACTT CTAGTGGAAA TGTGCAGGGC GCTGGCATCT CTCAAGAGAA GCCACCACCG 
GAAGGCATGC AGATTGAGGA CTCCAACTCC CAGGAACAGC AGAGCGACTC AAGGACGGAG 
ATACGCAGCT TCGAGACGTC ACCCTCGGCG ATACCTTTGC CGGTCGGACT TCAGGGCTTT 
AACTCCTCGC CGATGCCTCC AAGAGGACTG GGGGGACCTC CCATACGCTT CAGAGGACCG 
CCCCTATTTC CGTTTGATGG TCCACCGATG CCCGATGGAA GACCTGGAAT GGACTTTCCA 
GGTCCGAGAC TGCTGGGGCC CGATCCTAGG ATGATGGGAC CGCCTTCGGA CTTCAGACAG 
GGAAGCCCTC ATGAGCATCG AATGTTGGGT GGTCCCATGG AGCAGCGGAT GATGGGTGGG 
GCAGAACAGC CGATGATGCG AGGTCCCATG GAGCAAAGGC TGTTGGGCCC TCCAGAACAG 
ATGATGGGTG GGCCTCCGGA CTCGCGGATG TTGAGGGGTC CCCCAGAGCA AAGGTTTCTG 
GGCATGCCGC AGGAGCCAAG GATGCTGGGA GGTCCTTTGT CACCAAGAAT GCTAGGGGGT 
CCTCCGGATG ATAGACTAAT GGGTGGTCCA CCAGATCAGA GGATGATGGG AGGACCACGG 
GAGCACAGAA TGTTGGGCGG TCCACCTCAC GAGCATAGAA TGATGGGTGG TCCTCCTGAA 
CACAGAATGA TGGGTGGGCC CCCAGACCAC CGGATGTTGG GTGGGCCCCT GGATCATAGG 
ATGATGGGTG GGCCCCCTGA CCACCGAATG TTGGGTGGAC CGCTGGACCA CAGAATGATG 
GGAGGCCCGC CAGACCATAG GATGTTAAAC ATTCCTCCGG AGCACAGGAT GATGGGCAGG 
CCAGGAGATT TAGAACACCG GATGATGGGA GGTCCCCCTG AACACCGGAT GAGGGGGGGT 
CCACCAGACC ATCGGATGAT GGGAGGTCCT CCGGATCATA GGATGATGGG CGGGCCTCCC 
GACCACAGGA TGATGGGCAG TCCACCTGAA CACAGAATGC TTCCAGGACC TCAAGACCAG 
AGGATGATGG GAGGTCCACC GGACCACCGA ATGCTCGGAC CTCCCGATTT CATGATGGGT 
GGACCGCCGG AGTTTCGAAA GCTTGGACCG CCGGACTCGC GAATGTTGGG ACCGGGACCT 
CGAATGCTGG GACCCAATTT CGGCATGATG AGCCCGAATC AGATGGGGCC CCATGGTCCA 
GGCGGAGCAG ACTCGGACCC GGCAGATAAG ATGGCAAATT CTGATGGCAA GAAGGTGGCA 
ACTGACAAAG AGGAAGGCAA TGCCGGTGAA GGTGGTAAGG ACGCGCACTC GGGCGATAGG 
GAGGCAAATA TGGACGAAAA GAAAAACAGT CAAAACGAGC CAACCAAGAA CTGGAATCAC 
CAGGTGGGAG GCGCCGAAGA CGGCCGTGTA CCAAATTTAA TGGACGGTGG GCTGGGAAGA 
CCCGACGGCG ACAAGGGTCC CCAGAGCGAC GGCGATCGCG ACAGGAAAGG CTTTGGCTTT 
TTTGGGCCAC CCCGTACGAG GTTTGACGGT CCAGAAGAGC GGTCCGGCTT CAGACCTGCC 
TTCAACGATG GGCCAATGGA TCGCGGTTCC GATAACGCCT GGGGAGGCAG GGACGGCGAA 
GACGGCGGTG GGTTTGGCGG CGATCGCCGC GTTTTCACGA ATGCCTTCGG CGAAATGTCA 
GAAAGGCGGC GGAGCGGCGG TGGTGGCGGC GGCGGCAGTG GGGGCGGCAG TGGCGGTCGA 
GGGGAGCGTA GGAGCCGCTG GTCTCGGGAA GACAACGAGT GGGACTCGCG GCGTAGTGAT 
CGCGCAGATC GCAGTGATCG AAGCGATCGT GACCGAAGTG ATCGGGATCG GAACGACCGT 
GACCGCAGAG ACCGAGACCG TACCGACGGC AAAGACAGAA GCGACCGGGA TCGCTCGGAC 
AAGGACCGTA ACGATCGGGA AAGAAGTGAT CGGGATCGGA GCGACCGGGA TCGAAGCGAT 
CGTGACCGTA GTGATCGGGA CCGGAGTGAC CGAGACCGCA GCGAGAAAGA TCGGGACCGG 
AGTGAAAAGG ACCGTAGCGA CAGGGATCGC AGCGACAAAG ATCGCAGTGA AAAGGATCGC 
AGCGACAAGG ACCGCGGTGA CAGGGATCGA GACCGAAGCA AAGACCGGGA CCGAAGCAAA 
GATCGGGACC GTAGCAAAGA TCGGGACCGG AGCAAAGACC GAGATCGAAG TAGAGACCGC 
GATCGGAGTG ATAGGGATCG AGATCGGAGT GATAGAGAAA AGGGCGACAA GGATCGTGAG 
CGTAGCGACA AGGATCGTGA GCGCAGTGAT AAAGACCGTG GTGATCGTGA TCGGAGTGAG 
CGTGATCGCA GCAAAGGGGA TTACCGGAGA AGCCGCGAGA CTGACCGGGA GCGGGAACGG 
AGCTCAGGCC GGAGCCACAG CTCGTTAGAA CAAAGCTCAT CGGCTGTCGA GACCAACGGA 
GAGGTCTCCA AGGCCAACGG GAGCAGCTCA CAGTCGTCGA AAGAGGACAA GGCGCTAGGC 
AAGGCGCTAG ACAGGGCGCT GGACAATGTG CTGGACAAGG CTGAGGCACC CAAGCCCCAG 
GAAGTGGAGG AGGTCAGTGG GACGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029839109.1
CDS	93..5762
Translation	MEVVKAFNGELSSLYEGKPPISKAKMTAITKSAIKGIKFYKHIVQSVEKFVQKCRPEYKVPGLYVVDSIVRQSRHQFGQEKDVFAPRFTKNIHNTFQHLFKCPVEDRPKIVRVLNLWQKNQVFPPDVIQPLLDMASPSAVLAAGDMSEGPDMGLDSKPPSKAAMDQMASWKGWNLGGNSVQDNASAPPQETKTYALTQENLNLALAAEAEGASAPDPEMLVKLQSLASQLLGARALGSGNAIAEEKPSQPSAPAVKFNKKLLDFDYGDEEEEEEEEPVEETSTLAPPETNLEDSGGRPSEPNALALSMAQNLLNNPELLKQLQQMQQTLQQTEVLRGSETSAAPAAPSSLGTAALPQCSETGKAPSPHPQTPQGPDGQPLATTAGYLTASASVVNSLAPAVSSGLSQLFQTDFAGASGAPLLPESLSQLNMPLYPPAMAMPQGVPYGAGAIGPDAVSQGYPPAPQGVLAPPMGELSGLEGVPMPPAAGVAPPAYLSGGDMDERCTVLAPGGRDLDLRTGGLSPPRDRDRRRHSRSRSPRRRGRSRSRSPSQRTTPGARYNAAKRDRSREREKERERERDRRRKGLPPVKKDCLSVCSSTLWLGHVPKSVSETDLHDTFGEFGSIVKIEIIAPRGCAFVCMDRRQDAFRALQKLAKALKLQGSLIKMAWAPGKGVKSKELKDFWEVDLGVSYIPYEKLPKDVDLVSLEEGGVIDEDSLPEDLREKRRAQEQQRTQGGPGLAPSTAAGGGGADVVAAMAAFPGAAMTTPVVTTMAAPGGAATAVAMVPPPVAQFGIPLPPGMLPSQGLVMPQIPVPMGVPAPPNHMLMVPPHGTQTLLGHSALGLLGPPPLGISGTALGLSLHPPMSLPTTSQSAPVLPVATTAQLMAAAAAAAATTATVTTDTTATTATPMAIDGDATPTDEDGTSVMDSVSAIPTPADPPEQQGEASKDSVFGKLLPPPMSFPPLAAPPGVPSQFVTQGLVGAQKEPHPAEAKFKSPGSSQETSSGNVQGAGISQEKPPPEGMQIEDSNSQEQQSDSRTEIRSFETSPSAIPLPVGLQGFNSSPMPPRGLGGPPIRFRGPPLFPFDGPPMPDGRPGMDFPGPRLLGPDPRMMGPPSDFRQGSPHEHRMLGGPMEQRMMGGAEQPMMRGPMEQRLLGPPEQMMGGPPDSRMLRGPPEQRFLGMPQEPRMLGGPLSPRMLGGPPDDRLMGGPPDQRMMGGPREHRMLGGPPHEHRMMGGPPEHRMMGGPPDHRMLGGPLDHRMMGGPPDHRMLGGPLDHRMMGGPPDHRMLNIPPEHRMMGRPGDLEHRMMGGPPEHRMRGGPPDHRMMGGPPDHRMMGGPPDHRMMGSPPEHRMLPGPQDQRMMGGPPDHRMLGPPDFMMGGPPEFRKLGPPDSRMLGPGPRMLGPNFGMMSPNQMGPHGPGGADSDPADKMANSDGKKVATDKEEGNAGEGGKDAHSGDREANMDEKKNSQNEPTKNWNHQVGGAEDGRVPNLMDGGLGRPDGDKGPQSDGDRDRKGFGFFGPPRTRFDGPEERSGFRPAFNDGPMDRGSDNAWGGRDGEDGGGFGGDRRVFTNAFGEMSERRRSGGGGGGGSGGGSGGRGERRSRWSREDNEWDSRRSDRADRSDRSDRDRSDRDRNDRDRRDRDRTDGKDRSDRDRSDKDRNDRERSDRDRSDRDRSDRDRSDRDRSDRDRSEKDRDRSEKDRSDRDRSDKDRSEKDRSDKDRGDRDRDRSKDRDRSKDRDRSKDRDRSKDRDRSRDRDRSDRDRDRSDREKGDKDRERSDKDRERSDKDRGDRDRSERDRSKGDYRRSRETDRERERSSGRSHSSLEQSSSAVETNGEVSKANGSSSQSSKEDKALGKALDRALDNVLDKAEAPKPQEVEEVSGTA

Target ORF information:

RefSeq Version	XM_029983249.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis SR-related and CTD-associated factor 8 (LOC8031660), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029983249.1

ORF Insert Sequence:

ATGGAGGTTG TTAAAGCCTT CAACGGCGAG CTTTCCTCGC TGTATGAAGG CAAGCCTCCC 
ATCTCGAAAG CCAAGATGAC AGCAATTACA AAGTCTGCCA TCAAGGGCAT CAAATTCTAC 
AAACACATAG TTCAGAGCGT GGAGAAGTTT GTTCAGAAGT GCCGGCCCGA GTACAAGGTT 
CCAGGCCTGT ACGTTGTGGA CTCGATTGTG CGCCAGTCGC GGCACCAGTT TGGCCAGGAG 
AAGGATGTGT TCGCACCCCG GTTCACCAAG AACATCCACA ACACCTTCCA GCACCTCTTC 
AAGTGCCCCG TGGAGGACAG ACCCAAGATT GTGCGGGTGC TCAATTTGTG GCAGAAGAAC 
CAGGTGTTCC CCCCGGATGT CATCCAGCCG CTTTTGGACA TGGCGAGCCC CAGTGCGGTG 
TTGGCGGCAG GGGACATGAG CGAGGGGCCC GACATGGGCC TGGACTCAAA GCCCCCCAGC 
AAGGCGGCGA TGGACCAGAT GGCCTCCTGG AAGGGCTGGA ACCTGGGAGG CAACAGCGTC 
CAGGACAATG CCTCCGCCCC TCCACAGGAG ACCAAGACCT ACGCCCTGAC GCAGGAGAAC 
TTGAACCTGG CCCTGGCGGC AGAGGCGGAA GGCGCGAGCG CCCCGGACCC CGAGATGCTG 
GTGAAGCTGC AGAGCCTGGC GTCGCAGCTG CTGGGGGCGA GAGCGCTGGG AAGCGGGAAC 
GCCATTGCCG AAGAGAAGCC CAGTCAGCCC TCGGCCCCGG CCGTCAAGTT CAACAAGAAA 
CTGCTGGACT TTGACTACGG GGACGAAGAG GAAGAGGAGG AGGAGGAACC CGTGGAGGAA 
ACAAGCACCC TGGCCCCGCC CGAGACCAAC CTGGAGGACT CCGGAGGAAG GCCGTCAGAA 
CCCAATGCTC TGGCACTCAG CATGGCTCAG AACCTGCTGA ACAATCCAGA GTTGCTGAAG 
CAGCTGCAGC AGATGCAGCA GACCCTTCAG CAGACGGAGG TGCTTAGGGG CTCGGAGACG 
TCGGCCGCCC CCGCAGCCCC TTCGTCGCTC GGAACGGCCG CGCTCCCCCA GTGCTCGGAG 
ACTGGCAAGG CGCCCTCCCC TCATCCGCAG ACCCCGCAGG GGCCGGACGG GCAGCCGCTG 
GCGACGACTG CGGGGTACCT GACGGCCTCG GCCTCGGTGG TGAACTCCCT GGCGCCTGCG 
GTGAGCTCTG GGCTTTCCCA GCTTTTCCAG ACAGACTTTG CGGGGGCCTC TGGTGCTCCT 
TTGCTGCCTG AATCTCTGAG CCAGCTGAAC ATGCCCCTTT ACCCGCCGGC GATGGCGATG 
CCTCAGGGGG TCCCCTATGG GGCCGGTGCA ATAGGGCCGG ACGCCGTGTC CCAGGGGTAC 
CCGCCGGCCC CCCAAGGTGT GCTGGCCCCC CCCATGGGCG AGCTCTCCGG CCTGGAGGGC 
GTGCCCATGC CCCCCGCGGC CGGCGTGGCC CCGCCCGCGT ACCTGAGTGG GGGCGACATG 
GACGAGCGCT GCACTGTCTT GGCGCCCGGC GGAAGGGACC TGGACCTCAG GACAGGGGGT 
CTGTCCCCTC CCAGGGACAG AGATAGGCGG CGGCACAGTC GTTCCCGCTC TCCTCGGAGG 
CGTGGCCGTT CCCGGTCTCG GTCGCCCTCC CAGCGCACCA CGCCGGGTGC CCGGTACAAC 
GCCGCCAAGC GGGACCGGTC ACGCGAGCGG GAGAAGGAGC GGGAGCGGGA GAGGGACCGC 
CGGCGCAAGG GCCTGCCCCC CGTCAAGAAG GACTGCCTCA GCGTGTGCAG CTCGACCTTG 
TGGTTGGGAC ACGTGCCCAA GTCCGTGTCG GAGACGGACC TTCACGACAC CTTCGGCGAA 
TTTGGAAGCA TCGTCAAGAT TGAGATTATC GCCCCTCGTG GCTGTGCATT CGTGTGCATG 
GACCGGCGGC AGGATGCCTT TCGTGCCCTG CAGAAACTCG CAAAAGCACT CAAGCTTCAG 
GGGAGCCTCA TCAAGATGGC CTGGGCCCCT GGCAAGGGTG TGAAGAGCAA GGAGCTGAAG 
GACTTCTGGG AGGTTGACCT CGGCGTGAGC TACATCCCGT ACGAGAAGCT TCCCAAGGAC 
GTCGACCTCG TGTCCCTGGA AGAGGGCGGA GTCATCGACG AGGACTCTCT TCCTGAGGAC 
CTCAGAGAGA AGCGGAGGGC CCAGGAACAG CAGCGCACTC AGGGTGGGCC CGGTCTGGCA 
CCCTCCACGG CGGCCGGCGG CGGCGGTGCC GACGTGGTGG CCGCAATGGC AGCCTTTCCG 
GGGGCCGCCA TGACCACCCC AGTGGTGACC ACCATGGCTG CCCCCGGGGG TGCCGCCACT 
GCGGTGGCCA TGGTGCCGCC GCCCGTGGCA CAGTTTGGGA TCCCGCTGCC ACCAGGCATG 
CTGCCCTCGC AGGGCTTGGT GATGCCGCAG ATACCAGTGC CCATGGGGGT GCCCGCCCCT 
CCAAACCACA TGCTGATGGT GCCACCCCAC GGCACCCAAA CATTGCTGGG CCACAGTGCA 
CTGGGACTCC TCGGTCCACC GCCATTGGGA ATCTCAGGAA CTGCCCTGGG CCTTTCCCTG 
CACCCTCCCA TGAGCCTCCC GACCACCTCC CAGTCTGCCC CTGTGCTGCC CGTGGCAACA 
ACAGCACAGC TGATGGCGGC GGCGGCAGCT GCGGCGGCCA CGACGGCTAC GGTGACGACC 
GACACCACTG CGACCACTGC GACGCCCATG GCCATCGACG GGGATGCAAC GCCGACAGAC 
GAGGATGGAA CGAGTGTCAT GGACAGCGTC AGCGCCATTC CTACTCCGGC GGACCCTCCG 
GAACAACAAG GCGAGGCGTC CAAGGATTCA GTGTTTGGGA AACTTCTGCC ACCACCAATG 
TCTTTTCCGC CTCTCGCTGC GCCTCCAGGA GTTCCTTCGC AGTTCGTCAC TCAGGGACTG 
GTGGGTGCTC AGAAGGAACC GCACCCTGCC GAAGCTAAGT TCAAGTCTCC GGGATCTAGT 
CAAGAAACTT CTAGTGGAAA TGTGCAGGGC GCTGGCATCT CTCAAGAGAA GCCACCACCG 
GAAGGCATGC AGATTGAGGA CTCCAACTCC CAGGAACAGC AGAGCGACTC AAGGACGGAG 
ATACGCAGCT TCGAGACGTC ACCCTCGGCG ATACCTTTGC CGGTCGGACT TCAGGGCTTT 
AACTCCTCGC CGATGCCTCC AAGAGGACTG GGGGGACCTC CCATACGCTT CAGAGGACCG 
CCCCTATTTC CGTTTGATGG TCCACCGATG CCCGATGGAA GACCTGGAAT GGACTTTCCA 
GGTCCGAGAC TGCTGGGGCC CGATCCTAGG ATGATGGGAC CGCCTTCGGA CTTCAGACAG 
GGAAGCCCTC ATGAGCATCG AATGTTGGGT GGTCCCATGG AGCAGCGGAT GATGGGTGGG 
GCAGAACAGC CGATGATGCG AGGTCCCATG GAGCAAAGGC TGTTGGGCCC TCCAGAACAG 
ATGATGGGTG GGCCTCCGGA CTCGCGGATG TTGAGGGGTC CCCCAGAGCA AAGGTTTCTG 
GGCATGCCGC AGGAGCCAAG GATGCTGGGA GGTCCTTTGT CACCAAGAAT GCTAGGGGGT 
CCTCCGGATG ATAGACTAAT GGGTGGTCCA CCAGATCAGA GGATGATGGG AGGACCACGG 
GAGCACAGAA TGTTGGGCGG TCCACCTCAC GAGCATAGAA TGATGGGTGG TCCTCCTGAA 
CACAGAATGA TGGGTGGGCC CCCAGACCAC CGGATGTTGG GTGGGCCCCT GGATCATAGG 
ATGATGGGTG GGCCCCCTGA CCACCGAATG TTGGGTGGAC CGCTGGACCA CAGAATGATG 
GGAGGCCCGC CAGACCATAG GATGTTAAAC ATTCCTCCGG AGCACAGGAT GATGGGCAGG 
CCAGGAGATT TAGAACACCG GATGATGGGA GGTCCCCCTG AACACCGGAT GAGGGGGGGT 
CCACCAGACC ATCGGATGAT GGGAGGTCCT CCGGATCATA GGATGATGGG CGGGCCTCCC 
GACCACAGGA TGATGGGCAG TCCACCTGAA CACAGAATGC TTCCAGGACC TCAAGACCAG 
AGGATGATGG GAGGTCCACC GGACCACCGA ATGCTCGGAC CTCCCGATTT CATGATGGGT 
GGACCGCCGG AGTTTCGAAA GCTTGGACCG CCGGACTCGC GAATGTTGGG ACCGGGACCT 
CGAATGCTGG GACCCAATTT CGGCATGATG AGCCCGAATC AGATGGGGCC CCATGGTCCA 
GGCGGAGCAG ACTCGGACCC GGCAGATAAG ATGGCAAATT CTGATGGCAA GAAGGTGGCA 
ACTGACAAAG AGGAAGGCAA TGCCGGTGAA GGTGGTAAGG ACGCGCACTC GGGCGATAGG 
GAGGCAAATA TGGACGAAAA GAAAAACAGT CAAAACGAGC CAACCAAGAA CTGGAATCAC 
CAGGTGGGAG GCGCCGAAGA CGGCCGTGTA CCAAATTTAA TGGACGGTGG GCTGGGAAGA 
CCCGACGGCG ACAAGGGTCC CCAGAGCGAC GGCGATCGCG ACAGGAAAGG CTTTGGCTTT 
TTTGGGCCAC CCCGTACGAG GTTTGACGGT CCAGAAGAGC GGTCCGGCTT CAGACCTGCC 
TTCAACGATG GGCCAATGGA TCGCGGTTCC GATAACGCCT GGGGAGGCAG GGACGGCGAA 
GACGGCGGTG GGTTTGGCGG CGATCGCCGC GTTTTCACGA ATGCCTTCGG CGAAATGTCA 
GAAAGGCGGC GGAGCGGCGG TGGTGGCGGC GGCGGCAGTG GGGGCGGCAG TGGCGGTCGA 
GGGGAGCGTA GGAGCCGCTG GTCTCGGGAA GACAACGAGT GGGACTCGCG GCGTAGTGAT 
CGCGCAGATC GCAGTGATCG AAGCGATCGT GACCGAAGTG ATCGGGATCG GAACGACCGT 
GACCGCAGAG ACCGAGACCG TACCGACGGC AAAGACAGAA GCGACCGGGA TCGCTCGGAC 
AAGGACCGTA ACGATCGGGA AAGAAGTGAT CGGGATCGGA GCGACCGGGA TCGAAGCGAT 
CGTGACCGTA GTGATCGGGA CCGGAGTGAC CGAGACCGCA GCGAGAAAGA TCGGGACCGG 
AGTGAAAAGG ACCGTAGCGA CAGGGATCGC AGCGACAAAG ATCGCAGTGA AAAGGATCGC 
AGCGACAAGG ACCGCGGTGA CAGGGATCGA GACCGAAGCA AAGACCGGGA CCGAAGCAAA 
GATCGGGACC GTAGCAAAGA TCGGGACCGG AGCAAAGACC GAGATCGAAG TAGAGACCGC 
GATCGGAGTG ATAGGGATCG AGATCGGAGT GATAGAGAAA AGGGCGACAA GGATCGTGAG 
CGTAGCGACA AGGATCGTGA GCGCAGTGAT AAAGACCGTG GTGATCGTGA TCGGAGTGAG 
CGTGATCGCA GCAAAGGGGA TTACCGGAGA AGCCGCGAGA CTGACCGGGA GCGGGAACGG 
AGCTCAGGCC GGAGCCACAG CTCGTTAGAA CAAAGCTCAT CGGCTGTCGA GACCAACGGA 
GAGGTCTCCA AGGCCAACGG GAGCAGCTCA CAGTCGTCGA AAGAGGACAA GGCGCTAGGC 
AAGGCGCTAG ACAGGGCGCT GGACAATGTG CTGGACAAGG CTGAGGCACC CAAGCCCCAG 
GAAGTGGAGG AGGTCAGTGG GACGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8031660 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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