PRMT5 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	PRMT5
Entrez Gene ID	5068851
Full Name	putative arginine N-methyltransferase, type II
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001465396.1	XP_001465433.1	putative arginine N-methyltransferase, type II

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRMT5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRMT5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc02552	XM_001465396.1 Latest version!	Leishmania infantum JPCM5 putative arginine N-methyltransferase, type II (PRMT5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRMT5 ( XM_001465396.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001465433.1 Leishmania infantum JPCM5 putative arginine N-methyltransferase, type II (PRMT5), partial mRNA.

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CloneID	OLc02552
Clone ID Related Accession (Same CDS sequence)	XM_001465396.1
Accession Version	XM_001465396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-15
Organism	Leishmania infantum JPCM5
Product	putative arginine N-methyltransferase, type II
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009405). COMPLETENESS: incomplete on both ends.

ATGCATAGAC GCATACATAA AAACGGCGAA GTGTACACGC TCACGAAACA TCCTCCAAGT 
CGCTCCTCTT ATCTCCGCCT TCTACTATTC CCTCAGTCTC CCGGAATGTC GCTCGATGGC 
GGTGGCATCA GCAATGGCCC CGAGGCACAG GCTACCGCCT CGGCCATGGA GGAGGACTAC 
TGCCTGCATG GGCGGGAGCG CTTTCAAAAC CAGTGGGCGC GCGCTGGCGT GTTTTCTCCT 
GCCTCATTGC GTGTGTACGG CATCACCGGG GAGCCGGTGC TGATGCGCTC CATCAAGCAA 
GCGCGCATGC TGAGTGCCAC GTTCGGCACG TGCTTTCTCG GCCGCTTTCC GCGGTTCAGG 
TCGTCGCCGT CTTCGGGGGA CAGCGATGCC GCCGCCGTCA CGCAGAAGGA CAACGCCTCG 
AACGCTGCCG GTCCGTCCAG AAGCGGCAGC AGCTACGATT CGCTTCCCTT CAGCTCCCTC 
TTGCTGGGTC CGTGCCCTCC GCCGAACATG ACGGCTGATC CCCACATGCT ACATCAGGCG 
TGGCGCAGGG AGGTGGCCGG GACGTGGAGC GCGCTGCACA TGCCGTCGGA GTGGTGCCAG 
ACGATGAACG GTGTCGCGAC GGAGCTAAGC TGGTCGACGC GTGCGCAGCG GAAGCGTATC 
GCTGAGCAGC TTGAGCTAGC AGCCTTCATG TGCCTACGCG CCGTGCAGGT GCCGCTACCG 
TTCTATGCCG CGGACGTCGC ATGGTCTGGC GGAGATGACG ACGACAGCCG CGATCAGGAG 
GACAACGAGG GGGGTGCCAC AGCAGGCAAG CGCGGCGGCG CGCAGCCGAT GAGTGATGAA 
GAGGAGAATC AGCGCAACAT CCGCGTCGCT TGTCTTGCCG CGGATGTAGT GCTGTCTTTC 
ATTCAGCAGT GCACGAGGGT GAAGGTGTGG GTCCGCTGCG ATGCATGCAA CCCTGCCCAT 
CGCTACCAGC TGCAGCGTCT CCGCCAGGCC GTCCTTTACG GCTACTCCAC TCTCAAGACG 
GACTCAGGGC GGGTGATATA CCGCATCAAT ACACCCTGTA GTGTCACCAG CACCACTACG 
AGCCTCATGT CTGACGCATC ACCGCCGTCG TCGTCCTCGT CGCCGCGTTC GGGTGTGGTG 
CATACCGAGG CGGCTTCGTC TTTGATGGCG CTGCTCTACT ACTCCACGTG GAACCCGCGC 
ATGCTGCTGC CCAGCGAGTG GTTCGGCGAG GAGGTCGTCG GCTTTGAGTG CCCTACTGCC 
CCGGCGCTGC TGGCGTGTCT GGCGCCGCAC GGGGTGGCGC CGCACGAGCA CCCCAACTAC 
CGCCCTGACG CCCCAGGCAC CGCTTCCAGC ACGCACCATC GCCCCAGCGC CACTTTGGCG 
GAGGAGGACG TCGTGGAGGC GCAGGAGCAG GCGCACCCTA ACCACGGCAA CCCCGACGAG 
GACAACGCAC CGCTCCGGCG AACGCCACCG ACCTACCACG ACGGCGAGAG CGAAGGGAGT 
CGAACAGGCT ACTCACTCTT CGACTGGTAC GACAGCGTTA CAGAGCTTGA GACGAGCCAC 
GAAGCCGACT CGGCCGTGGC GGCGGCGAAG GGGGGCTCCA AGGGCTCCGG TTCCGCCTCC 
GACACATCGT ATGAGGCAGC GGCCGACTGT CGCCGCTCTC ATTTGCTGCG GAGCCGCTAC 
ACCTACGTGA TGCCGGAGGA TCTCCAGCCG TTGGTGGCCT CCCGCTGGGC TGGCACCTAC 
CCGCCCTTTG TCAGCGCGAT CACCTTCATT GTGGAGCTGC TGCGCCGGCG CGCGATGCCA 
GTGTTCGATC GCACCTTTTT TGACCAGTAC CCGCTGCTCA ACTACCTCCA CTTCAAAGGC 
GTCGAGGAGC CGACCCGCGA CGTCTTTGCC TCCTTCGAGG GTCAACTCCA ACTGCCACTA 
CAGCCACTGA GCCACCACCT GTCGAGCGGC GTCTACGAAG TCTTTGAGCG CGACGCACGA 
AAATACCGGC AGTACCGCGA GGCAGTCTTT CACTACGTGC GGGACTGGTA CGCCGCCGGG 
GCGGAGCAGC AGCACGCGCA TCAGAACAAA ATGTTCTTCG CGAAGCACGG TGCCATGCAG 
AGGGTGCCGG TGCCCTCGCC GGATGAGCGG ACACTCCACC TCGTGCTGCT CGGCTGCGGT 
CGCGGCCCGT TGATCGATGA GTGCCTCCAC GCTGTCTCCG CGCTCGGGGT GCGACTGCGC 
ATCTTCGCCA TCGAAAAGAA TCCCCCAGCT GCCGCCTTCA CGCGAATGCG GTGGGCGAAT 
GACCCCGAGT GGATGCAGCT GGCCTACACC TTCGGACACA CACTGGAGGT GATCGTCGCC 
GACGGGCGCA CCATTGCGAC CGCCGCGGAA GACGGCTCGC TCACCTTACC GGCTGACTTC 
GGTCTGTGCG ACCTTATCGT TTCTGAGCTG CTCGGGAGTC TGGGCGACAA CGAGCTGAGC 
CCGGAGTGTC TGGAGGCCTT CCACGCTCAG CTCGAGGACA TCCAGCTCAG CCGCGGCATC 
GCTTTCAACC CTCACCTCAT GTGCATTCCA CAGCAATACA CTGCGTGGGT GGCGCCGCTC 
ATGTCTGCCA CCTTTGATGC GGCGGTGACG GAGGCAGCTG TGAAAGGGTT GACGGTGCCG 
CCGCCGGGCT GCCACGACCG CCATGCTGCT CTCAATCATA CGCTGCTTGT CACAAACCTC 
AGCAGGGCTG TCACACTGGC GCCTCCGCAG CCGTGCTGGA CCTTCGAGCA CCGATTCCAC 
GGCGGCAGCG GTAACGACTA CAAGGGAGAC CGCGGCGCGA TGAAGCGGCG GGAGCCCGTG 
TCGCTGGAGC GGGCGGCGAG CCTTGTCTTT CAGGTGCCGC CGTGTGGCCG CTGCTGCGGC 
TTGGCTGGCT ATTTCTCCGC GGTGCTTTAC GAGTCTGCGA CCGCGCCAGC CACGATCATC 
GCCACTGCCC CCGTGGAGAG GACGGAGGAC ATGTACAGTT GGTTTCCATG CGTCTTTGCG 
CTCGAGCCCG CCCAGCAGGC GGAGCTGCAG GATGTCGGGC AAGCAGCGGC GGAAGAAAGC 
CGCATGGTCG CCATCCGCGT GCAGCTTGAT CGGCGGACGG GTCTGGCGGA GCAGAGGGTT 
TGGTATGAGT GGAGCGTCAC CTACGGCGAT GCGGCGGTGG AGCGACAGTC GCCGAGGACT 
GGCCGCGACG CCTCCGCTGC ACCGTTGGTT CACAACAAGA ACGGCTGGGC TGCGTCGATG 
CTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001465433.1
CDS	1..3249
Translation	MHRRIHKNGEVYTLTKHPPSRSSYLRLLLFPQSPGMSLDGGGISNGPEAQATASAMEEDYCLHGRERFQNQWARAGVFSPASLRVYGITGEPVLMRSIKQARMLSATFGTCFLGRFPRFRSSPSSGDSDAAAVTQKDNASNAAGPSRSGSSYDSLPFSSLLLGPCPPPNMTADPHMLHQAWRREVAGTWSALHMPSEWCQTMNGVATELSWSTRAQRKRIAEQLELAAFMCLRAVQVPLPFYAADVAWSGGDDDDSRDQEDNEGGATAGKRGGAQPMSDEEENQRNIRVACLAADVVLSFIQQCTRVKVWVRCDACNPAHRYQLQRLRQAVLYGYSTLKTDSGRVIYRINTPCSVTSTTTSLMSDASPPSSSSSPRSGVVHTEAASSLMALLYYSTWNPRMLLPSEWFGEEVVGFECPTAPALLACLAPHGVAPHEHPNYRPDAPGTASSTHHRPSATLAEEDVVEAQEQAHPNHGNPDEDNAPLRRTPPTYHDGESEGSRTGYSLFDWYDSVTELETSHEADSAVAAAKGGSKGSGSASDTSYEAAADCRRSHLLRSRYTYVMPEDLQPLVASRWAGTYPPFVSAITFIVELLRRRAMPVFDRTFFDQYPLLNYLHFKGVEEPTRDVFASFEGQLQLPLQPLSHHLSSGVYEVFERDARKYRQYREAVFHYVRDWYAAGAEQQHAHQNKMFFAKHGAMQRVPVPSPDERTLHLVLLGCGRGPLIDECLHAVSALGVRLRIFAIEKNPPAAAFTRMRWANDPEWMQLAYTFGHTLEVIVADGRTIATAAEDGSLTLPADFGLCDLIVSELLGSLGDNELSPECLEAFHAQLEDIQLSRGIAFNPHLMCIPQQYTAWVAPLMSATFDAAVTEAAVKGLTVPPPGCHDRHAALNHTLLVTNLSRAVTLAPPQPCWTFEHRFHGGSGNDYKGDRGAMKRREPVSLERAASLVFQVPPCGRCCGLAGYFSAVLYESATAPATIIATAPVERTEDMYSWFPCVFALEPAQQAELQDVGQAAAEESRMVAIRVQLDRRTGLAEQRVWYEWSVTYGDAAVERQSPRTGRDASAAPLVHNKNGWAASMLL

Target ORF information:

RefSeq Version	XM_001465396.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative arginine N-methyltransferase, type II (PRMT5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001465396.1

ORF Insert Sequence:

ATGCATAGAC GCATACATAA AAACGGCGAA GTGTACACGC TCACGAAACA TCCTCCAAGT 
CGCTCCTCTT ATCTCCGCCT TCTACTATTC CCTCAGTCTC CCGGAATGTC GCTCGATGGC 
GGTGGCATCA GCAATGGCCC CGAGGCACAG GCTACCGCCT CGGCCATGGA GGAGGACTAC 
TGCCTGCATG GGCGGGAGCG CTTTCAAAAC CAGTGGGCGC GCGCTGGCGT GTTTTCTCCT 
GCCTCATTGC GTGTGTACGG CATCACCGGG GAGCCGGTGC TGATGCGCTC CATCAAGCAA 
GCGCGCATGC TGAGTGCCAC GTTCGGCACG TGCTTTCTCG GCCGCTTTCC GCGGTTCAGG 
TCGTCGCCGT CTTCGGGGGA CAGCGATGCC GCCGCCGTCA CGCAGAAGGA CAACGCCTCG 
AACGCTGCCG GTCCGTCCAG AAGCGGCAGC AGCTACGATT CGCTTCCCTT CAGCTCCCTC 
TTGCTGGGTC CGTGCCCTCC GCCGAACATG ACGGCTGATC CCCACATGCT ACATCAGGCG 
TGGCGCAGGG AGGTGGCCGG GACGTGGAGC GCGCTGCACA TGCCGTCGGA GTGGTGCCAG 
ACGATGAACG GTGTCGCGAC GGAGCTAAGC TGGTCGACGC GTGCGCAGCG GAAGCGTATC 
GCTGAGCAGC TTGAGCTAGC AGCCTTCATG TGCCTACGCG CCGTGCAGGT GCCGCTACCG 
TTCTATGCCG CGGACGTCGC ATGGTCTGGC GGAGATGACG ACGACAGCCG CGATCAGGAG 
GACAACGAGG GGGGTGCCAC AGCAGGCAAG CGCGGCGGCG CGCAGCCGAT GAGTGATGAA 
GAGGAGAATC AGCGCAACAT CCGCGTCGCT TGTCTTGCCG CGGATGTAGT GCTGTCTTTC 
ATTCAGCAGT GCACGAGGGT GAAGGTGTGG GTCCGCTGCG ATGCATGCAA CCCTGCCCAT 
CGCTACCAGC TGCAGCGTCT CCGCCAGGCC GTCCTTTACG GCTACTCCAC TCTCAAGACG 
GACTCAGGGC GGGTGATATA CCGCATCAAT ACACCCTGTA GTGTCACCAG CACCACTACG 
AGCCTCATGT CTGACGCATC ACCGCCGTCG TCGTCCTCGT CGCCGCGTTC GGGTGTGGTG 
CATACCGAGG CGGCTTCGTC TTTGATGGCG CTGCTCTACT ACTCCACGTG GAACCCGCGC 
ATGCTGCTGC CCAGCGAGTG GTTCGGCGAG GAGGTCGTCG GCTTTGAGTG CCCTACTGCC 
CCGGCGCTGC TGGCGTGTCT GGCGCCGCAC GGGGTGGCGC CGCACGAGCA CCCCAACTAC 
CGCCCTGACG CCCCAGGCAC CGCTTCCAGC ACGCACCATC GCCCCAGCGC CACTTTGGCG 
GAGGAGGACG TCGTGGAGGC GCAGGAGCAG GCGCACCCTA ACCACGGCAA CCCCGACGAG 
GACAACGCAC CGCTCCGGCG AACGCCACCG ACCTACCACG ACGGCGAGAG CGAAGGGAGT 
CGAACAGGCT ACTCACTCTT CGACTGGTAC GACAGCGTTA CAGAGCTTGA GACGAGCCAC 
GAAGCCGACT CGGCCGTGGC GGCGGCGAAG GGGGGCTCCA AGGGCTCCGG TTCCGCCTCC 
GACACATCGT ATGAGGCAGC GGCCGACTGT CGCCGCTCTC ATTTGCTGCG GAGCCGCTAC 
ACCTACGTGA TGCCGGAGGA TCTCCAGCCG TTGGTGGCCT CCCGCTGGGC TGGCACCTAC 
CCGCCCTTTG TCAGCGCGAT CACCTTCATT GTGGAGCTGC TGCGCCGGCG CGCGATGCCA 
GTGTTCGATC GCACCTTTTT TGACCAGTAC CCGCTGCTCA ACTACCTCCA CTTCAAAGGC 
GTCGAGGAGC CGACCCGCGA CGTCTTTGCC TCCTTCGAGG GTCAACTCCA ACTGCCACTA 
CAGCCACTGA GCCACCACCT GTCGAGCGGC GTCTACGAAG TCTTTGAGCG CGACGCACGA 
AAATACCGGC AGTACCGCGA GGCAGTCTTT CACTACGTGC GGGACTGGTA CGCCGCCGGG 
GCGGAGCAGC AGCACGCGCA TCAGAACAAA ATGTTCTTCG CGAAGCACGG TGCCATGCAG 
AGGGTGCCGG TGCCCTCGCC GGATGAGCGG ACACTCCACC TCGTGCTGCT CGGCTGCGGT 
CGCGGCCCGT TGATCGATGA GTGCCTCCAC GCTGTCTCCG CGCTCGGGGT GCGACTGCGC 
ATCTTCGCCA TCGAAAAGAA TCCCCCAGCT GCCGCCTTCA CGCGAATGCG GTGGGCGAAT 
GACCCCGAGT GGATGCAGCT GGCCTACACC TTCGGACACA CACTGGAGGT GATCGTCGCC 
GACGGGCGCA CCATTGCGAC CGCCGCGGAA GACGGCTCGC TCACCTTACC GGCTGACTTC 
GGTCTGTGCG ACCTTATCGT TTCTGAGCTG CTCGGGAGTC TGGGCGACAA CGAGCTGAGC 
CCGGAGTGTC TGGAGGCCTT CCACGCTCAG CTCGAGGACA TCCAGCTCAG CCGCGGCATC 
GCTTTCAACC CTCACCTCAT GTGCATTCCA CAGCAATACA CTGCGTGGGT GGCGCCGCTC 
ATGTCTGCCA CCTTTGATGC GGCGGTGACG GAGGCAGCTG TGAAAGGGTT GACGGTGCCG 
CCGCCGGGCT GCCACGACCG CCATGCTGCT CTCAATCATA CGCTGCTTGT CACAAACCTC 
AGCAGGGCTG TCACACTGGC GCCTCCGCAG CCGTGCTGGA CCTTCGAGCA CCGATTCCAC 
GGCGGCAGCG GTAACGACTA CAAGGGAGAC CGCGGCGCGA TGAAGCGGCG GGAGCCCGTG 
TCGCTGGAGC GGGCGGCGAG CCTTGTCTTT CAGGTGCCGC CGTGTGGCCG CTGCTGCGGC 
TTGGCTGGCT ATTTCTCCGC GGTGCTTTAC GAGTCTGCGA CCGCGCCAGC CACGATCATC 
GCCACTGCCC CCGTGGAGAG GACGGAGGAC ATGTACAGTT GGTTTCCATG CGTCTTTGCG 
CTCGAGCCCG CCCAGCAGGC GGAGCTGCAG GATGTCGGGC AAGCAGCGGC GGAAGAAAGC 
CGCATGGTCG CCATCCGCGT GCAGCTTGAT CGGCGGACGG GTCTGGCGGA GCAGAGGGTT 
TGGTATGAGT GGAGCGTCAC CTACGGCGAT GCGGCGGTGG AGCGACAGTC GCCGAGGACT 
GGCCGCGACG CCTCCGCTGC ACCGTTGGTT CACAACAAGA ACGGCTGGGC TGCGTCGATG 
CTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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PRMT5 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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