LOC100880175 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100880175
Entrez Gene ID	100880175
Full Name	pleiotropic regulator 1
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012284301.1	XP_012139691.1	pleiotropic regulator 1

Pathways from BioSystems
Gene Ontology

The following LOC100880175 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100880175 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe97094	XM_012284301.1 Latest version!	Megachile rotundata pleiotropic regulator 1 (LOC100880175), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100880175 ( XM_012284301.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012139691.1 Megachile rotundata pleiotropic regulator 1 (LOC100880175), mRNA.

Price & Availability↑

CloneID	OMe97094
Clone ID Related Accession (Same CDS sequence)	XM_012284301.1
Accession Version	XM_012284301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	pleiotropic regulator 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797069.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTAC AAAAGCATTC TGTGCATACT CTAGTATTCC GATCTTTAAA AAGAACGCAC 
GATATGTTCT TATTAAATCA AGGTACTTTA CCACCTGTGG ATCTTAATTT ACAAAAGATG 
AAAAAAACCA TAAAAGCAAA GGACTCTTAT GGTCCTGTAT TGGAACGTGT CAAGAATAAC 
AATGTGATGA AAGCACAGAA AGAAAATGAA AATGCAGATC CTCCTCCACC AGGCGATGAA 
TCTTTTGGAG GAAATAGTAT TACTGCTACT GTACCTTATA ATAGCACACA AAATAGTAAT 
ATAATAGCAA CAACACAAGC AAGTTCTGGA GGAAATGTAA TAAATACTCT TATAACACAA 
AAAAAGACAA CTTCTATGGC AAAACCAAAA TGGCATCCGC CATGGAAATT ATACAGAGTT 
ATTAGTGGCC ACCTTGGTTG GGTTAGGTGT TGTGCCGTTG AACCAGGAAA TGAATGGTTT 
GCTACTGGTT CAGCAGATAG AGTAATTAAA ATATGGGATC TTGCAAGTGG TAAATTGAAA 
GTTTCTTTGA CTGGTCACAT AAGTAGTGTC CGTGGACTTG CATTTTCTCA GCGTCATCCG 
TACCTATTTT CCTGTGGAGA GGATCGACAA GTAAAGTGTT GGGATCTTGA ATACAACAAG 
GTTATAAGAC ACTACCATGG TCATTTATCA GCTGTATATT CAATGGCACT CCATCCTAGT 
ATAGATGTAT TAGTAACTGC GGGTAGAGAT TCTACTGCAA GAGTATGGGA TATGCGTACC 
AAGGCAAATG TACACACGCT TGTTGGTCAT ACTAATACAA TTGCTAGTGT AATTTGTCAA 
ACAGCTGAGC CACAGATTGT CACTGGAAGT CATGATTGCA CAATAAGGTT ATGGGATCTA 
GCTACAGGAA AATCTAAAGC AACTTTAACA AATCACAAAA AGAGTGTGAG AGCTGTGACT 
TTCCATCCAT CTTTGTACAT GTTTGCATCA GCATCTCCAG ATAATATTAA ACAGTGGAAA 
TGTCCAGAAG GCAAATTTAT TCAAAACTTA TCAGGTCATA ATGCCATAGT TAATTGCTTG 
GCTGTTAATG CTGATGGTGT TTTAGTTTCT GGAGCAGATA ATGGTACAAT GCATCTGTGG 
GATTGGAGGA CAGGATATAA TTTTCAACGA TTACAAGCAC CAGTTCAACC TGGTTCAATG 
GATAGTGAAG CTGGAGTATT TAGTATTACA TTTGACATGT CTGGCACACG AATGATTACA 
ACAGAAGCAG ATAAAACAAT CAAAGTGTAT AAAGAAGATG ATACTGCTAC GGAGGAAACG 
CATCCTGTAA ATTGGAGACC AGATATAATA AAACGAAGGA AATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012139691.1
CDS	175..1542
Translation	MDVQKHSVHTLVFRSLKRTHDMFLLNQGTLPPVDLNLQKMKKTIKAKDSYGPVLERVKNNNVMKAQKENENADPPPPGDESFGGNSITATVPYNSTQNSNIIATTQASSGGNVINTLITQKKTTSMAKPKWHPPWKLYRVISGHLGWVRCCAVEPGNEWFATGSADRVIKIWDLASGKLKVSLTGHISSVRGLAFSQRHPYLFSCGEDRQVKCWDLEYNKVIRHYHGHLSAVYSMALHPSIDVLVTAGRDSTARVWDMRTKANVHTLVGHTNTIASVICQTAEPQIVTGSHDCTIRLWDLATGKSKATLTNHKKSVRAVTFHPSLYMFASASPDNIKQWKCPEGKFIQNLSGHNAIVNCLAVNADGVLVSGADNGTMHLWDWRTGYNFQRLQAPVQPGSMDSEAGVFSITFDMSGTRMITTEADKTIKVYKEDDTATEETHPVNWRPDIIKRRKY

Target ORF information:

RefSeq Version	XM_012284301.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata pleiotropic regulator 1 (LOC100880175), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012284301.1

ORF Insert Sequence:

ATGGACGTAC AAAAGCATTC TGTGCATACT CTAGTATTCC GATCTTTAAA AAGAACGCAC 
GATATGTTCT TATTAAATCA AGGTACTTTA CCACCTGTGG ATCTTAATTT ACAAAAGATG 
AAAAAAACCA TAAAAGCAAA GGACTCTTAT GGTCCTGTAT TGGAACGTGT CAAGAATAAC 
AATGTGATGA AAGCACAGAA AGAAAATGAA AATGCAGATC CTCCTCCACC AGGCGATGAA 
TCTTTTGGAG GAAATAGTAT TACTGCTACT GTACCTTATA ATAGCACACA AAATAGTAAT 
ATAATAGCAA CAACACAAGC AAGTTCTGGA GGAAATGTAA TAAATACTCT TATAACACAA 
AAAAAGACAA CTTCTATGGC AAAACCAAAA TGGCATCCGC CATGGAAATT ATACAGAGTT 
ATTAGTGGCC ACCTTGGTTG GGTTAGGTGT TGTGCCGTTG AACCAGGAAA TGAATGGTTT 
GCTACTGGTT CAGCAGATAG AGTAATTAAA ATATGGGATC TTGCAAGTGG TAAATTGAAA 
GTTTCTTTGA CTGGTCACAT AAGTAGTGTC CGTGGACTTG CATTTTCTCA GCGTCATCCG 
TACCTATTTT CCTGTGGAGA GGATCGACAA GTAAAGTGTT GGGATCTTGA ATACAACAAG 
GTTATAAGAC ACTACCATGG TCATTTATCA GCTGTATATT CAATGGCACT CCATCCTAGT 
ATAGATGTAT TAGTAACTGC GGGTAGAGAT TCTACTGCAA GAGTATGGGA TATGCGTACC 
AAGGCAAATG TACACACGCT TGTTGGTCAT ACTAATACAA TTGCTAGTGT AATTTGTCAA 
ACAGCTGAGC CACAGATTGT CACTGGAAGT CATGATTGCA CAATAAGGTT ATGGGATCTA 
GCTACAGGAA AATCTAAAGC AACTTTAACA AATCACAAAA AGAGTGTGAG AGCTGTGACT 
TTCCATCCAT CTTTGTACAT GTTTGCATCA GCATCTCCAG ATAATATTAA ACAGTGGAAA 
TGTCCAGAAG GCAAATTTAT TCAAAACTTA TCAGGTCATA ATGCCATAGT TAATTGCTTG 
GCTGTTAATG CTGATGGTGT TTTAGTTTCT GGAGCAGATA ATGGTACAAT GCATCTGTGG 
GATTGGAGGA CAGGATATAA TTTTCAACGA TTACAAGCAC CAGTTCAACC TGGTTCAATG 
GATAGTGAAG CTGGAGTATT TAGTATTACA TTTGACATGT CTGGCACACG AATGATTACA 
ACAGAAGCAG ATAAAACAAT CAAAGTGTAT AAAGAAGATG ATACTGCTAC GGAGGAAACG 
CATCCTGTAA ATTGGAGACC AGATATAATA AAACGAAGGA AATATTAA