LOC100881256 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100881256
Entrez Gene ID	100881256
Full Name	proteasome-associated protein ECM29 homolog
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012295986.1	XP_012151376.1	proteasome-associated protein ECM29 homolog isoform X1
XM_012295987.1	XP_012151377.1	proteasome-associated protein ECM29 homolog isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100881256 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100881256 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe113999	XM_012295986.1 Latest version!	Megachile rotundata proteasome-associated protein ECM29 homolog (LOC100881256), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMe114000	XM_012295987.1 Latest version!	Megachile rotundata proteasome-associated protein ECM29 homolog (LOC100881256), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100881256 ( XM_012295986.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012151376.1 Megachile rotundata proteasome-associated protein ECM29 homolog (LOC100881256), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe113999
Clone ID Related Accession (Same CDS sequence)	XM_012295986.1
Accession Version	XM_012295986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	proteasome-associated protein ECM29 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797704.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCG CTACGGACGA ACTGATCTTG CTTGAACGAG TATTTCTTCG CTTGGGTTCT 
GTGGAGACTG ATGAACAGTT ACAAGCATCA GTATGTAAAT TTCTGCCTCC AGTTTTACTA 
AAACTATCTA GTGCACAAGA AGGTGTTAGA AAAAAAGTAA TGGAATTATT AATTCATATA 
AATAAAAGAG TAAAAACTAG ACCACAAGTT CAACTGCCAG TTGAAGCTCT ACTATTACAA 
TATCAAGATC CTGCTGCCAG TTCATTCGTA ATTAATTTTA CAATTATATA TATAAAACTA 
GGATATCCCA GAATGGACAT ACAAAAGCAG GCTGAACTAA TTCCAAGTGT ACTAAATGCT 
ATAGAAGGAA AACCATTATC TCATCAAGAT AGTTTATTAC TTATAATTAT GCCTGCATTG 
GGTCATATTA ATGTTCCCAT GGATCCAGAT AAACGAGCAT CTCTTCTTGG TTTGCAAGAT 
AAACCTTATG TATCAAAACA ATTAATCAAT TTTATGCTTG ATGTTTTGTT ATTACCATAT 
GGATCAGTAG GACAAACAGA AAATCAACAT CCAGGTCAAG CTATTGATTG GTCACAGTTT 
CCTGTACCAC CAGGATTAAG TGAATATGCA TTTAAACGAG TAATAGGTGA AAATCCACCA 
ACAGCTGAAC AACTGGAACA AACTAAATTA GGAATTGTCA AATTTCTTAG TAGTGGATTC 
TTTCCCGATT CTGACATTCT CATACATTTA ATAGTAGCAG CTGCTGATAC CAGATTCAGT 
GTTGCTAATA TGGCAGATTT AGAATTGAAA AAAATTGTTA ATACATTAGA TTGGTCTTCA 
ATGCAGTTAG CAGCTCCACT TTATACTTTA TTTTTGGGTA CTAATGCTTT AGCTGCACAG 
AAAGAAGTTA AATCAGAAAT GAAACGATTA CCAGCAAGTA CGCGAATTCG TCTCAAATTG 
TTACATTATC TTTGTCGTGT AACTAAAACT GGTTTTATTA TACCACCTTG TATTCAGGTA 
GTCTTTGATT CACTGTACGG TAAAAATACA AACACAAAAT TAAAATCAAT GGCATTGACA 
TTTACATCAA ATATTGTACA ACAGTGTAAC CTAGCGCCAC TATCTCGTGT AGCTGGAGTT 
ATTTTAAATG GTATGATAAA GTTAATTTCT GAAGGTGAAC ATAATCATAA AGCAATGGCA 
TATACTGTTA TTGGACAATT GGGACAAAGA ATACCATCGT TAATTAATAA GGATGTAAAT 
TTATTACGTA ATTTATTCCA AACACTGATA TCGACGGAAG GTGATTTACG AAGAGCAGTA 
CGAGATGCGT TAATTACGGT CACGTCTGCA TTCGTACTTA GTAAAGACGA TGAAACTAAT 
ATATCTTTAT TGAATGAATT ATTGTCTACT TATATCGAAT CACCAGAATA TAATGTCAGA 
TTTGTAGCTA TGCATTATGC TGCTACAGTA TTCCCACCTG ATGATGCACC TTCTCGTTAT 
CTTCTGTTGC TGGCATGTGG TGATAATAAA CATGAAATAA GTAAAGAAGC CGCAAAAGTT 
CTGTACAGCA CTTTGAATAA AACTGAAAAT GATAAGCAAG ATAGTAACCA AATTTTATTG 
CCAGACTTCT CAAAACTTGT TGTATATATT TATTCTAAAA TGCAAACGAG AATGCCAACT 
TCAAGTACTG GTAAAGTGAA TGTTGAAAGT AAGATACTTC CTTACAGTAT TACTGTATTT 
ACAGAGATAA TTAATTATCT TCGTATCTGT TTAGCAAAAA GTGCTAATGC TACCGTACAA 
AATGAACCAT TACAGCATCC CTGTGAAAGT ACACCTTTAA TTGCACGTTA TTTAGAAAAT 
CTTTATAAAG AAGAACCATA TACAGTAAAC AGTTATTTTG ATATGGTTTT GATCTTTAGT 
AATGCCTCTG CAGATCAGAC TTCTTTACAA GCTTTGTTAG AACTGGTTAG TTCTATTCCA 
CAATATATAA CGAAAATTTA CGAAAAACAG TTGTCACGAC TTCATAATTT ACTAACGTCT 
ACAAAAGTAG ATATAAGACA AGTGGCTGCT AAAATTTATG GCGTAATAAC TGCCCCACTT 
CCGGTTAATG ACTTCGAAAC TCAAATTTCT GAAATTATAA ATATTACAAA TAAAAAGAAT 
TTGGAAGCTC AACACGGAGG ATTATTAGCT TTAACATATA TGATGGAAAG ACGATTATTA 
ATGTTTAGAA AGAATTCTAC AAATGATTCA TTTAATTGGG ATCTTTATAC TAATGTTGTA 
AAAATAATAT GTAATTTTCT TGAAAGTAGT ACAATTTTGT TAATGGATGC TGCAATTCAG 
AGTATTGGTA TTTTGGGAAA AATTTGTGGA TTACCATTAC CTAATGATGG TGAAGAACAA 
CCCAATAAAA AAGCAATTGT GGAAATATTG TTTTCCACAT TGATTAACGC AAAATTAAAT 
ACTAAGATTA AAGAAAAGGC TGCACTTTCA CTAGGATATT TGTGCGTTGG AGAAAATTTT 
CCACATACCT CACAGATAAT TGACAAAATA ATTGGAATTG TTAAAGAAAC GAAAGATATT 
GAAATTCATC TAATTTTGGG AGAAGCATTA GTTTGCTGTG TTCAAGGTGA AGCCTCTCCA 
GAGAAAAGGG ATCCTTGGAC AACTTTACCG AACGAACATG AAGTACCTTA TAGTAACACC 
AGTACTGAAC TATTTATGCA GACGTTAGAT AGATTACTTG GAATGTATAA AGATCCACAC 
CCTAATTTAA GACAGGCGGT CTGTGTATGG TTGTTAGCAC TGTTAAAATA TAATATCCAA 
AGAGAATGCA TTAAACAAAG ATTTTCGTCG TTACACCGTG CATTCATGGA TTACCTTGCA 
GATGACAGTG ATGTAATACA GGATATAGCA GCAAAAGGAC TTAGTTTGAT ATACGTTAAT 
ATTAACAAAG AAGAAAAAGA TTCTTTAGTT TCTACTATAT TAAGTCAATT TACACAAGGG 
CGTAGAAGTG TACAAGAAGT TACACCCGAC ACAAAATTGT TTGAAGAGGG TCAATTGGGA 
AAGACTCCAA CAAGTGGCAA CTTAACTACA TATAGAGAAA TCTGTTCTTT GGCTACAGAA 
TTACAGAAAC CAGATCTAGT ATATTACTTT ATGCACTTAG CAAATCACAA TGCAGTGTGG 
TCATCTAAAA AAGGTGCTGC ATTTGGATTT GCAACGATTG CAAAAATAGC AAATGAAGAA 
TTAAATAAAT ACTTGCCAAG CATTGTACCA CGCTTGTATA GATATCAGTA TGATCCAACT 
CCTAAAATTC AACAAAGCAT GTCTAGTATT TGGCGTGCAA TCATTCCATC GACAAGTAAA 
GCAATTGAAC AGTATCATGA AGAAATATTA GATGATATAA CTAATAATTT AACACATTAC 
GAATGGCGAG TTCGAATTAG TTGTTGCAAT GCGCTTGCAG ATCTATTAAG AGCAAATGCT 
CGTTTAAATC TTGTAAAATA TGCACCAGAA TTGTGGAAAA AATTGTTTCG AGTAATGGAT 
GATATTCACG AAGGTACGCG ATTAGCTGCT ACGAATACAA CTAAAATACT TAGTAAAGTT 
TGCATCCGAT ATTGTGATTC GTCTCATGGT AAAGACGGAG AAGAAGTACT GCAAGCAATA 
TTGCCTATAT TATTAGATAT TGGAGTTATT CACATATTAA GCTCCGTAAG ATCGATATCG 
CTGCAAACAG TATCTCAATT GATTTCAAGA GCTGGTAATT TACTTAAACC ATCTTTAGTC 
ACTTTAATTC CTGCATTGTT AAGTGCAATT GGCGATTCGG AAAGTCCTAA TTTGTCGTAC 
TGGAGTAATG TATGTGGGAC GGCAACAGAA ACACGAGACG TTATTGATAA TATTAGAGCT 
AGTGCTGCTA AAGGACATTA TGCTACTGAA ACAGTAACGA AGTGCATACA GTACATCGAT 
GCAGAGGTTT TAAAAGAATT AATGCCAAAG GTGATAGAAT TAATTAAATC TAACGTGAAT 
TTTGGAACGA AAATTACTTG CTTACACTTC ATTATTCTTC TGAGTGTTCA TTTTAAACAT 
GAAATACAGC CTTACACTGG TAAAATTTTG TCAACTTTAC TGAATGGATT ATTAGATTGC 
AATTCTGTTG TTAGGAAAAA TAATGCAAGT ACTATTGGGC ATATAGTTGG ATCAATGAAG 
GAATCAAGTC TTAACAAGTT GTTTAAAACA CTTAATACAT GGTACATGGA ACGAGATGAT 
ACGGTCAAGT TTGCAATTGG ACAAACATTA CAAGCTATAA ACAATTATAA TCAAGATGTA 
CTAAGAAATT ATTCAAATAT TGTTATACCT CTTACTTTTC TCGCAATGCA TGAACAAAAA 
TCACAAGAGA ATGAAAATGC AATTGAATTA TGGACCGATC TATGGAACGA CATAACTCCA 
GGAACTGAAG CTGGAATCAG GCAAAATGTT GAACCAATAA CAAACATTTT ACGTTCATCT 
TTAGAATCAG CTTCTTGGAT TACAAAAGCA CAAGCAGCTA ATGCAATCAA TACTTTAGCG 
CAAAAATTGG GTAATGATCT TGATGCAACT GTAAGAGACA CTTTGTTAAA GGTGTTAATC 
GATGGTTTAC GCGGAAGAAC TTGGGACGGC AAGGAACGAT TGTTACATGC TCTTGCTACG 
CTGACATGTA ATAGCAAGCA AGGTCTTAAA GAGAATGCTG AGATATCTGC AGATATAGTG 
CAAGTGTTAT ATAAAGAAAC AAAAAAGGAG GCTTTAGAAT ATCGCCGTCA TGCTTTGAAA 
GCATTTGGTA TGGTTTTGCA TGAATTAGAC GTCGATAAGT TTAAGGAAGT GTACGATATC 
GCTCAAGAAA TTTGGACAAC GTTGCCAAAG AAAGACGAAA CCGAATCTCT AATATCTGAT 
GAGAGAATGA AGAGAACCGA TGACATGGTG AAATTATACG AAGCCATTTT TGAAACACTT 
GGAAAAGCAT GGCCATCTTG TAAAGAAACT CAAGATAAAT ATTGTATAGA ACTTATAACA 
TGTTGTGACA AAATGTTTCC TCTTCAGTCT ACAACTATAC AAATATCAAT GTTAGTAATG 
CTTAATTTAT TTTTGGATAA ACTTTCTTTG CTCAAAACAA ATTACTCAGA ATTGTCAATC 
AAAGATAAAG AAATGTTGGA TTCAATTTGT AACACGCTTA ATAATATATT AACTCGTAGT 
ATAGGTATTT CGTACATCAG GATAAGAAAA GAAGCCTTGA ACATAGTTCT GTCCCTTGGT 
AACAAGTTAC GTGATACTAA AAATACTGAA CTATTTGATA AAATGACTTT TATAATGCAG 
AAAGTCTTAC CAGAACTAAC AAAAGATAAT GAACCGGAAA TTCGTACAAG ACTTATTGAT 
ATCAAAGAGA TGTTTAAAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012151376.1
CDS	610..6033
Translation	MAAATDELILLERVFLRLGSVETDEQLQASVCKFLPPVLLKLSSAQEGVRKKVMELLIHINKRVKTRPQVQLPVEALLLQYQDPAASSFVINFTIIYIKLGYPRMDIQKQAELIPSVLNAIEGKPLSHQDSLLLIIMPALGHINVPMDPDKRASLLGLQDKPYVSKQLINFMLDVLLLPYGSVGQTENQHPGQAIDWSQFPVPPGLSEYAFKRVIGENPPTAEQLEQTKLGIVKFLSSGFFPDSDILIHLIVAAADTRFSVANMADLELKKIVNTLDWSSMQLAAPLYTLFLGTNALAAQKEVKSEMKRLPASTRIRLKLLHYLCRVTKTGFIIPPCIQVVFDSLYGKNTNTKLKSMALTFTSNIVQQCNLAPLSRVAGVILNGMIKLISEGEHNHKAMAYTVIGQLGQRIPSLINKDVNLLRNLFQTLISTEGDLRRAVRDALITVTSAFVLSKDDETNISLLNELLSTYIESPEYNVRFVAMHYAATVFPPDDAPSRYLLLLACGDNKHEISKEAAKVLYSTLNKTENDKQDSNQILLPDFSKLVVYIYSKMQTRMPTSSTGKVNVESKILPYSITVFTEIINYLRICLAKSANATVQNEPLQHPCESTPLIARYLENLYKEEPYTVNSYFDMVLIFSNASADQTSLQALLELVSSIPQYITKIYEKQLSRLHNLLTSTKVDIRQVAAKIYGVITAPLPVNDFETQISEIINITNKKNLEAQHGGLLALTYMMERRLLMFRKNSTNDSFNWDLYTNVVKIICNFLESSTILLMDAAIQSIGILGKICGLPLPNDGEEQPNKKAIVEILFSTLINAKLNTKIKEKAALSLGYLCVGENFPHTSQIIDKIIGIVKETKDIEIHLILGEALVCCVQGEASPEKRDPWTTLPNEHEVPYSNTSTELFMQTLDRLLGMYKDPHPNLRQAVCVWLLALLKYNIQRECIKQRFSSLHRAFMDYLADDSDVIQDIAAKGLSLIYVNINKEEKDSLVSTILSQFTQGRRSVQEVTPDTKLFEEGQLGKTPTSGNLTTYREICSLATELQKPDLVYYFMHLANHNAVWSSKKGAAFGFATIAKIANEELNKYLPSIVPRLYRYQYDPTPKIQQSMSSIWRAIIPSTSKAIEQYHEEILDDITNNLTHYEWRVRISCCNALADLLRANARLNLVKYAPELWKKLFRVMDDIHEGTRLAATNTTKILSKVCIRYCDSSHGKDGEEVLQAILPILLDIGVIHILSSVRSISLQTVSQLISRAGNLLKPSLVTLIPALLSAIGDSESPNLSYWSNVCGTATETRDVIDNIRASAAKGHYATETVTKCIQYIDAEVLKELMPKVIELIKSNVNFGTKITCLHFIILLSVHFKHEIQPYTGKILSTLLNGLLDCNSVVRKNNASTIGHIVGSMKESSLNKLFKTLNTWYMERDDTVKFAIGQTLQAINNYNQDVLRNYSNIVIPLTFLAMHEQKSQENENAIELWTDLWNDITPGTEAGIRQNVEPITNILRSSLESASWITKAQAANAINTLAQKLGNDLDATVRDTLLKVLIDGLRGRTWDGKERLLHALATLTCNSKQGLKENAEISADIVQVLYKETKKEALEYRRHALKAFGMVLHELDVDKFKEVYDIAQEIWTTLPKKDETESLISDERMKRTDDMVKLYEAIFETLGKAWPSCKETQDKYCIELITCCDKMFPLQSTTIQISMLVMLNLFLDKLSLLKTNYSELSIKDKEMLDSICNTLNNILTRSIGISYIRIRKEALNIVLSLGNKLRDTKNTELFDKMTFIMQKVLPELTKDNEPEIRTRLIDIKEMFKI

Target ORF information:

RefSeq Version	XM_012295986.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata proteasome-associated protein ECM29 homolog (LOC100881256), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012295986.1

ORF Insert Sequence:

ATGGCAGCCG CTACGGACGA ACTGATCTTG CTTGAACGAG TATTTCTTCG CTTGGGTTCT 
GTGGAGACTG ATGAACAGTT ACAAGCATCA GTATGTAAAT TTCTGCCTCC AGTTTTACTA 
AAACTATCTA GTGCACAAGA AGGTGTTAGA AAAAAAGTAA TGGAATTATT AATTCATATA 
AATAAAAGAG TAAAAACTAG ACCACAAGTT CAACTGCCAG TTGAAGCTCT ACTATTACAA 
TATCAAGATC CTGCTGCCAG TTCATTCGTA ATTAATTTTA CAATTATATA TATAAAACTA 
GGATATCCCA GAATGGACAT ACAAAAGCAG GCTGAACTAA TTCCAAGTGT ACTAAATGCT 
ATAGAAGGAA AACCATTATC TCATCAAGAT AGTTTATTAC TTATAATTAT GCCTGCATTG 
GGTCATATTA ATGTTCCCAT GGATCCAGAT AAACGAGCAT CTCTTCTTGG TTTGCAAGAT 
AAACCTTATG TATCAAAACA ATTAATCAAT TTTATGCTTG ATGTTTTGTT ATTACCATAT 
GGATCAGTAG GACAAACAGA AAATCAACAT CCAGGTCAAG CTATTGATTG GTCACAGTTT 
CCTGTACCAC CAGGATTAAG TGAATATGCA TTTAAACGAG TAATAGGTGA AAATCCACCA 
ACAGCTGAAC AACTGGAACA AACTAAATTA GGAATTGTCA AATTTCTTAG TAGTGGATTC 
TTTCCCGATT CTGACATTCT CATACATTTA ATAGTAGCAG CTGCTGATAC CAGATTCAGT 
GTTGCTAATA TGGCAGATTT AGAATTGAAA AAAATTGTTA ATACATTAGA TTGGTCTTCA 
ATGCAGTTAG CAGCTCCACT TTATACTTTA TTTTTGGGTA CTAATGCTTT AGCTGCACAG 
AAAGAAGTTA AATCAGAAAT GAAACGATTA CCAGCAAGTA CGCGAATTCG TCTCAAATTG 
TTACATTATC TTTGTCGTGT AACTAAAACT GGTTTTATTA TACCACCTTG TATTCAGGTA 
GTCTTTGATT CACTGTACGG TAAAAATACA AACACAAAAT TAAAATCAAT GGCATTGACA 
TTTACATCAA ATATTGTACA ACAGTGTAAC CTAGCGCCAC TATCTCGTGT AGCTGGAGTT 
ATTTTAAATG GTATGATAAA GTTAATTTCT GAAGGTGAAC ATAATCATAA AGCAATGGCA 
TATACTGTTA TTGGACAATT GGGACAAAGA ATACCATCGT TAATTAATAA GGATGTAAAT 
TTATTACGTA ATTTATTCCA AACACTGATA TCGACGGAAG GTGATTTACG AAGAGCAGTA 
CGAGATGCGT TAATTACGGT CACGTCTGCA TTCGTACTTA GTAAAGACGA TGAAACTAAT 
ATATCTTTAT TGAATGAATT ATTGTCTACT TATATCGAAT CACCAGAATA TAATGTCAGA 
TTTGTAGCTA TGCATTATGC TGCTACAGTA TTCCCACCTG ATGATGCACC TTCTCGTTAT 
CTTCTGTTGC TGGCATGTGG TGATAATAAA CATGAAATAA GTAAAGAAGC CGCAAAAGTT 
CTGTACAGCA CTTTGAATAA AACTGAAAAT GATAAGCAAG ATAGTAACCA AATTTTATTG 
CCAGACTTCT CAAAACTTGT TGTATATATT TATTCTAAAA TGCAAACGAG AATGCCAACT 
TCAAGTACTG GTAAAGTGAA TGTTGAAAGT AAGATACTTC CTTACAGTAT TACTGTATTT 
ACAGAGATAA TTAATTATCT TCGTATCTGT TTAGCAAAAA GTGCTAATGC TACCGTACAA 
AATGAACCAT TACAGCATCC CTGTGAAAGT ACACCTTTAA TTGCACGTTA TTTAGAAAAT 
CTTTATAAAG AAGAACCATA TACAGTAAAC AGTTATTTTG ATATGGTTTT GATCTTTAGT 
AATGCCTCTG CAGATCAGAC TTCTTTACAA GCTTTGTTAG AACTGGTTAG TTCTATTCCA 
CAATATATAA CGAAAATTTA CGAAAAACAG TTGTCACGAC TTCATAATTT ACTAACGTCT 
ACAAAAGTAG ATATAAGACA AGTGGCTGCT AAAATTTATG GCGTAATAAC TGCCCCACTT 
CCGGTTAATG ACTTCGAAAC TCAAATTTCT GAAATTATAA ATATTACAAA TAAAAAGAAT 
TTGGAAGCTC AACACGGAGG ATTATTAGCT TTAACATATA TGATGGAAAG ACGATTATTA 
ATGTTTAGAA AGAATTCTAC AAATGATTCA TTTAATTGGG ATCTTTATAC TAATGTTGTA 
AAAATAATAT GTAATTTTCT TGAAAGTAGT ACAATTTTGT TAATGGATGC TGCAATTCAG 
AGTATTGGTA TTTTGGGAAA AATTTGTGGA TTACCATTAC CTAATGATGG TGAAGAACAA 
CCCAATAAAA AAGCAATTGT GGAAATATTG TTTTCCACAT TGATTAACGC AAAATTAAAT 
ACTAAGATTA AAGAAAAGGC TGCACTTTCA CTAGGATATT TGTGCGTTGG AGAAAATTTT 
CCACATACCT CACAGATAAT TGACAAAATA ATTGGAATTG TTAAAGAAAC GAAAGATATT 
GAAATTCATC TAATTTTGGG AGAAGCATTA GTTTGCTGTG TTCAAGGTGA AGCCTCTCCA 
GAGAAAAGGG ATCCTTGGAC AACTTTACCG AACGAACATG AAGTACCTTA TAGTAACACC 
AGTACTGAAC TATTTATGCA GACGTTAGAT AGATTACTTG GAATGTATAA AGATCCACAC 
CCTAATTTAA GACAGGCGGT CTGTGTATGG TTGTTAGCAC TGTTAAAATA TAATATCCAA 
AGAGAATGCA TTAAACAAAG ATTTTCGTCG TTACACCGTG CATTCATGGA TTACCTTGCA 
GATGACAGTG ATGTAATACA GGATATAGCA GCAAAAGGAC TTAGTTTGAT ATACGTTAAT 
ATTAACAAAG AAGAAAAAGA TTCTTTAGTT TCTACTATAT TAAGTCAATT TACACAAGGG 
CGTAGAAGTG TACAAGAAGT TACACCCGAC ACAAAATTGT TTGAAGAGGG TCAATTGGGA 
AAGACTCCAA CAAGTGGCAA CTTAACTACA TATAGAGAAA TCTGTTCTTT GGCTACAGAA 
TTACAGAAAC CAGATCTAGT ATATTACTTT ATGCACTTAG CAAATCACAA TGCAGTGTGG 
TCATCTAAAA AAGGTGCTGC ATTTGGATTT GCAACGATTG CAAAAATAGC AAATGAAGAA 
TTAAATAAAT ACTTGCCAAG CATTGTACCA CGCTTGTATA GATATCAGTA TGATCCAACT 
CCTAAAATTC AACAAAGCAT GTCTAGTATT TGGCGTGCAA TCATTCCATC GACAAGTAAA 
GCAATTGAAC AGTATCATGA AGAAATATTA GATGATATAA CTAATAATTT AACACATTAC 
GAATGGCGAG TTCGAATTAG TTGTTGCAAT GCGCTTGCAG ATCTATTAAG AGCAAATGCT 
CGTTTAAATC TTGTAAAATA TGCACCAGAA TTGTGGAAAA AATTGTTTCG AGTAATGGAT 
GATATTCACG AAGGTACGCG ATTAGCTGCT ACGAATACAA CTAAAATACT TAGTAAAGTT 
TGCATCCGAT ATTGTGATTC GTCTCATGGT AAAGACGGAG AAGAAGTACT GCAAGCAATA 
TTGCCTATAT TATTAGATAT TGGAGTTATT CACATATTAA GCTCCGTAAG ATCGATATCG 
CTGCAAACAG TATCTCAATT GATTTCAAGA GCTGGTAATT TACTTAAACC ATCTTTAGTC 
ACTTTAATTC CTGCATTGTT AAGTGCAATT GGCGATTCGG AAAGTCCTAA TTTGTCGTAC 
TGGAGTAATG TATGTGGGAC GGCAACAGAA ACACGAGACG TTATTGATAA TATTAGAGCT 
AGTGCTGCTA AAGGACATTA TGCTACTGAA ACAGTAACGA AGTGCATACA GTACATCGAT 
GCAGAGGTTT TAAAAGAATT AATGCCAAAG GTGATAGAAT TAATTAAATC TAACGTGAAT 
TTTGGAACGA AAATTACTTG CTTACACTTC ATTATTCTTC TGAGTGTTCA TTTTAAACAT 
GAAATACAGC CTTACACTGG TAAAATTTTG TCAACTTTAC TGAATGGATT ATTAGATTGC 
AATTCTGTTG TTAGGAAAAA TAATGCAAGT ACTATTGGGC ATATAGTTGG ATCAATGAAG 
GAATCAAGTC TTAACAAGTT GTTTAAAACA CTTAATACAT GGTACATGGA ACGAGATGAT 
ACGGTCAAGT TTGCAATTGG ACAAACATTA CAAGCTATAA ACAATTATAA TCAAGATGTA 
CTAAGAAATT ATTCAAATAT TGTTATACCT CTTACTTTTC TCGCAATGCA TGAACAAAAA 
TCACAAGAGA ATGAAAATGC AATTGAATTA TGGACCGATC TATGGAACGA CATAACTCCA 
GGAACTGAAG CTGGAATCAG GCAAAATGTT GAACCAATAA CAAACATTTT ACGTTCATCT 
TTAGAATCAG CTTCTTGGAT TACAAAAGCA CAAGCAGCTA ATGCAATCAA TACTTTAGCG 
CAAAAATTGG GTAATGATCT TGATGCAACT GTAAGAGACA CTTTGTTAAA GGTGTTAATC 
GATGGTTTAC GCGGAAGAAC TTGGGACGGC AAGGAACGAT TGTTACATGC TCTTGCTACG 
CTGACATGTA ATAGCAAGCA AGGTCTTAAA GAGAATGCTG AGATATCTGC AGATATAGTG 
CAAGTGTTAT ATAAAGAAAC AAAAAAGGAG GCTTTAGAAT ATCGCCGTCA TGCTTTGAAA 
GCATTTGGTA TGGTTTTGCA TGAATTAGAC GTCGATAAGT TTAAGGAAGT GTACGATATC 
GCTCAAGAAA TTTGGACAAC GTTGCCAAAG AAAGACGAAA CCGAATCTCT AATATCTGAT 
GAGAGAATGA AGAGAACCGA TGACATGGTG AAATTATACG AAGCCATTTT TGAAACACTT 
GGAAAAGCAT GGCCATCTTG TAAAGAAACT CAAGATAAAT ATTGTATAGA ACTTATAACA 
TGTTGTGACA AAATGTTTCC TCTTCAGTCT ACAACTATAC AAATATCAAT GTTAGTAATG 
CTTAATTTAT TTTTGGATAA ACTTTCTTTG CTCAAAACAA ATTACTCAGA ATTGTCAATC 
AAAGATAAAG AAATGTTGGA TTCAATTTGT AACACGCTTA ATAATATATT AACTCGTAGT 
ATAGGTATTT CGTACATCAG GATAAGAAAA GAAGCCTTGA ACATAGTTCT GTCCCTTGGT 
AACAAGTTAC GTGATACTAA AAATACTGAA CTATTTGATA AAATGACTTT TATAATGCAG 
AAAGTCTTAC CAGAACTAAC AAAAGATAAT GAACCGGAAA TTCGTACAAG ACTTATTGAT 
ATCAAAGAGA TGTTTAAAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100881256 ( XM_012295987.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012151377.1 Megachile rotundata proteasome-associated protein ECM29 homolog (LOC100881256), transcript variant X2, mRNA.

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CloneID	OMe114000
Clone ID Related Accession (Same CDS sequence)	XM_012295987.1
Accession Version	XM_012295987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	proteasome-associated protein ECM29 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797704.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATAC AAAAGCAGGC TGAACTAATT CCAAGTGTAC TAAATGCTAT AGAAGGAAAA 
CCATTATCTC ATCAAGATAG TTTATTACTT ATAATTATGC CTGCATTGGG TCATATTAAT 
GTTCCCATGG ATCCAGATAA ACGAGCATCT CTTCTTGGTT TGCAAGATAA ACCTTATGTA 
TCAAAACAAT TAATCAATTT TATGCTTGAT GTTTTGTTAT TACCATATGG ATCAGTAGGA 
CAAACAGAAA ATCAACATCC AGGTCAAGCT ATTGATTGGT CACAGTTTCC TGTACCACCA 
GGATTAAGTG AATATGCATT TAAACGAGTA ATAGGTGAAA ATCCACCAAC AGCTGAACAA 
CTGGAACAAA CTAAATTAGG AATTGTCAAA TTTCTTAGTA GTGGATTCTT TCCCGATTCT 
GACATTCTCA TACATTTAAT AGTAGCAGCT GCTGATACCA GATTCAGTGT TGCTAATATG 
GCAGATTTAG AATTGAAAAA AATTGTTAAT ACATTAGATT GGTCTTCAAT GCAGTTAGCA 
GCTCCACTTT ATACTTTATT TTTGGGTACT AATGCTTTAG CTGCACAGAA AGAAGTTAAA 
TCAGAAATGA AACGATTACC AGCAAGTACG CGAATTCGTC TCAAATTGTT ACATTATCTT 
TGTCGTGTAA CTAAAACTGG TTTTATTATA CCACCTTGTA TTCAGGTAGT CTTTGATTCA 
CTGTACGGTA AAAATACAAA CACAAAATTA AAATCAATGG CATTGACATT TACATCAAAT 
ATTGTACAAC AGTGTAACCT AGCGCCACTA TCTCGTGTAG CTGGAGTTAT TTTAAATGGT 
ATGATAAAGT TAATTTCTGA AGGTGAACAT AATCATAAAG CAATGGCATA TACTGTTATT 
GGACAATTGG GACAAAGAAT ACCATCGTTA ATTAATAAGG ATGTAAATTT ATTACGTAAT 
TTATTCCAAA CACTGATATC GACGGAAGGT GATTTACGAA GAGCAGTACG AGATGCGTTA 
ATTACGGTCA CGTCTGCATT CGTACTTAGT AAAGACGATG AAACTAATAT ATCTTTATTG 
AATGAATTAT TGTCTACTTA TATCGAATCA CCAGAATATA ATGTCAGATT TGTAGCTATG 
CATTATGCTG CTACAGTATT CCCACCTGAT GATGCACCTT CTCGTTATCT TCTGTTGCTG 
GCATGTGGTG ATAATAAACA TGAAATAAGT AAAGAAGCCG CAAAAGTTCT GTACAGCACT 
TTGAATAAAA CTGAAAATGA TAAGCAAGAT AGTAACCAAA TTTTATTGCC AGACTTCTCA 
AAACTTGTTG TATATATTTA TTCTAAAATG CAAACGAGAA TGCCAACTTC AAGTACTGGT 
AAAGTGAATG TTGAAAGTAA GATACTTCCT TACAGTATTA CTGTATTTAC AGAGATAATT 
AATTATCTTC GTATCTGTTT AGCAAAAAGT GCTAATGCTA CCGTACAAAA TGAACCATTA 
CAGCATCCCT GTGAAAGTAC ACCTTTAATT GCACGTTATT TAGAAAATCT TTATAAAGAA 
GAACCATATA CAGTAAACAG TTATTTTGAT ATGGTTTTGA TCTTTAGTAA TGCCTCTGCA 
GATCAGACTT CTTTACAAGC TTTGTTAGAA CTGGTTAGTT CTATTCCACA ATATATAACG 
AAAATTTACG AAAAACAGTT GTCACGACTT CATAATTTAC TAACGTCTAC AAAAGTAGAT 
ATAAGACAAG TGGCTGCTAA AATTTATGGC GTAATAACTG CCCCACTTCC GGTTAATGAC 
TTCGAAACTC AAATTTCTGA AATTATAAAT ATTACAAATA AAAAGAATTT GGAAGCTCAA 
CACGGAGGAT TATTAGCTTT AACATATATG ATGGAAAGAC GATTATTAAT GTTTAGAAAG 
AATTCTACAA ATGATTCATT TAATTGGGAT CTTTATACTA ATGTTGTAAA AATAATATGT 
AATTTTCTTG AAAGTAGTAC AATTTTGTTA ATGGATGCTG CAATTCAGAG TATTGGTATT 
TTGGGAAAAA TTTGTGGATT ACCATTACCT AATGATGGTG AAGAACAACC CAATAAAAAA 
GCAATTGTGG AAATATTGTT TTCCACATTG ATTAACGCAA AATTAAATAC TAAGATTAAA 
GAAAAGGCTG CACTTTCACT AGGATATTTG TGCGTTGGAG AAAATTTTCC ACATACCTCA 
CAGATAATTG ACAAAATAAT TGGAATTGTT AAAGAAACGA AAGATATTGA AATTCATCTA 
ATTTTGGGAG AAGCATTAGT TTGCTGTGTT CAAGGTGAAG CCTCTCCAGA GAAAAGGGAT 
CCTTGGACAA CTTTACCGAA CGAACATGAA GTACCTTATA GTAACACCAG TACTGAACTA 
TTTATGCAGA CGTTAGATAG ATTACTTGGA ATGTATAAAG ATCCACACCC TAATTTAAGA 
CAGGCGGTCT GTGTATGGTT GTTAGCACTG TTAAAATATA ATATCCAAAG AGAATGCATT 
AAACAAAGAT TTTCGTCGTT ACACCGTGCA TTCATGGATT ACCTTGCAGA TGACAGTGAT 
GTAATACAGG ATATAGCAGC AAAAGGACTT AGTTTGATAT ACGTTAATAT TAACAAAGAA 
GAAAAAGATT CTTTAGTTTC TACTATATTA AGTCAATTTA CACAAGGGCG TAGAAGTGTA 
CAAGAAGTTA CACCCGACAC AAAATTGTTT GAAGAGGGTC AATTGGGAAA GACTCCAACA 
AGTGGCAACT TAACTACATA TAGAGAAATC TGTTCTTTGG CTACAGAATT ACAGAAACCA 
GATCTAGTAT ATTACTTTAT GCACTTAGCA AATCACAATG CAGTGTGGTC ATCTAAAAAA 
GGTGCTGCAT TTGGATTTGC AACGATTGCA AAAATAGCAA ATGAAGAATT AAATAAATAC 
TTGCCAAGCA TTGTACCACG CTTGTATAGA TATCAGTATG ATCCAACTCC TAAAATTCAA 
CAAAGCATGT CTAGTATTTG GCGTGCAATC ATTCCATCGA CAAGTAAAGC AATTGAACAG 
TATCATGAAG AAATATTAGA TGATATAACT AATAATTTAA CACATTACGA ATGGCGAGTT 
CGAATTAGTT GTTGCAATGC GCTTGCAGAT CTATTAAGAG CAAATGCTCG TTTAAATCTT 
GTAAAATATG CACCAGAATT GTGGAAAAAA TTGTTTCGAG TAATGGATGA TATTCACGAA 
GGTACGCGAT TAGCTGCTAC GAATACAACT AAAATACTTA GTAAAGTTTG CATCCGATAT 
TGTGATTCGT CTCATGGTAA AGACGGAGAA GAAGTACTGC AAGCAATATT GCCTATATTA 
TTAGATATTG GAGTTATTCA CATATTAAGC TCCGTAAGAT CGATATCGCT GCAAACAGTA 
TCTCAATTGA TTTCAAGAGC TGGTAATTTA CTTAAACCAT CTTTAGTCAC TTTAATTCCT 
GCATTGTTAA GTGCAATTGG CGATTCGGAA AGTCCTAATT TGTCGTACTG GAGTAATGTA 
TGTGGGACGG CAACAGAAAC ACGAGACGTT ATTGATAATA TTAGAGCTAG TGCTGCTAAA 
GGACATTATG CTACTGAAAC AGTAACGAAG TGCATACAGT ACATCGATGC AGAGGTTTTA 
AAAGAATTAA TGCCAAAGGT GATAGAATTA ATTAAATCTA ACGTGAATTT TGGAACGAAA 
ATTACTTGCT TACACTTCAT TATTCTTCTG AGTGTTCATT TTAAACATGA AATACAGCCT 
TACACTGGTA AAATTTTGTC AACTTTACTG AATGGATTAT TAGATTGCAA TTCTGTTGTT 
AGGAAAAATA ATGCAAGTAC TATTGGGCAT ATAGTTGGAT CAATGAAGGA ATCAAGTCTT 
AACAAGTTGT TTAAAACACT TAATACATGG TACATGGAAC GAGATGATAC GGTCAAGTTT 
GCAATTGGAC AAACATTACA AGCTATAAAC AATTATAATC AAGATGTACT AAGAAATTAT 
TCAAATATTG TTATACCTCT TACTTTTCTC GCAATGCATG AACAAAAATC ACAAGAGAAT 
GAAAATGCAA TTGAATTATG GACCGATCTA TGGAACGACA TAACTCCAGG AACTGAAGCT 
GGAATCAGGC AAAATGTTGA ACCAATAACA AACATTTTAC GTTCATCTTT AGAATCAGCT 
TCTTGGATTA CAAAAGCACA AGCAGCTAAT GCAATCAATA CTTTAGCGCA AAAATTGGGT 
AATGATCTTG ATGCAACTGT AAGAGACACT TTGTTAAAGG TGTTAATCGA TGGTTTACGC 
GGAAGAACTT GGGACGGCAA GGAACGATTG TTACATGCTC TTGCTACGCT GACATGTAAT 
AGCAAGCAAG GTCTTAAAGA GAATGCTGAG ATATCTGCAG ATATAGTGCA AGTGTTATAT 
AAAGAAACAA AAAAGGAGGC TTTAGAATAT CGCCGTCATG CTTTGAAAGC ATTTGGTATG 
GTTTTGCATG AATTAGACGT CGATAAGTTT AAGGAAGTGT ACGATATCGC TCAAGAAATT 
TGGACAACGT TGCCAAAGAA AGACGAAACC GAATCTCTAA TATCTGATGA GAGAATGAAG 
AGAACCGATG ACATGGTGAA ATTATACGAA GCCATTTTTG AAACACTTGG AAAAGCATGG 
CCATCTTGTA AAGAAACTCA AGATAAATAT TGTATAGAAC TTATAACATG TTGTGACAAA 
ATGTTTCCTC TTCAGTCTAC AACTATACAA ATATCAATGT TAGTAATGCT TAATTTATTT 
TTGGATAAAC TTTCTTTGCT CAAAACAAAT TACTCAGAAT TGTCAATCAA AGATAAAGAA 
ATGTTGGATT CAATTTGTAA CACGCTTAAT AATATATTAA CTCGTAGTAT AGGTATTTCG 
TACATCAGGA TAAGAAAAGA AGCCTTGAAC ATAGTTCTGT CCCTTGGTAA CAAGTTACGT 
GATACTAAAA ATACTGAACT ATTTGATAAA ATGACTTTTA TAATGCAGAA AGTCTTACCA 
GAACTAACAA AAGATAATGA ACCGGAAATT CGTACAAGAC TTATTGATAT CAAAGAGATG 
TTTAAAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012151377.1
CDS	101..5212
Translation	MDIQKQAELIPSVLNAIEGKPLSHQDSLLLIIMPALGHINVPMDPDKRASLLGLQDKPYVSKQLINFMLDVLLLPYGSVGQTENQHPGQAIDWSQFPVPPGLSEYAFKRVIGENPPTAEQLEQTKLGIVKFLSSGFFPDSDILIHLIVAAADTRFSVANMADLELKKIVNTLDWSSMQLAAPLYTLFLGTNALAAQKEVKSEMKRLPASTRIRLKLLHYLCRVTKTGFIIPPCIQVVFDSLYGKNTNTKLKSMALTFTSNIVQQCNLAPLSRVAGVILNGMIKLISEGEHNHKAMAYTVIGQLGQRIPSLINKDVNLLRNLFQTLISTEGDLRRAVRDALITVTSAFVLSKDDETNISLLNELLSTYIESPEYNVRFVAMHYAATVFPPDDAPSRYLLLLACGDNKHEISKEAAKVLYSTLNKTENDKQDSNQILLPDFSKLVVYIYSKMQTRMPTSSTGKVNVESKILPYSITVFTEIINYLRICLAKSANATVQNEPLQHPCESTPLIARYLENLYKEEPYTVNSYFDMVLIFSNASADQTSLQALLELVSSIPQYITKIYEKQLSRLHNLLTSTKVDIRQVAAKIYGVITAPLPVNDFETQISEIINITNKKNLEAQHGGLLALTYMMERRLLMFRKNSTNDSFNWDLYTNVVKIICNFLESSTILLMDAAIQSIGILGKICGLPLPNDGEEQPNKKAIVEILFSTLINAKLNTKIKEKAALSLGYLCVGENFPHTSQIIDKIIGIVKETKDIEIHLILGEALVCCVQGEASPEKRDPWTTLPNEHEVPYSNTSTELFMQTLDRLLGMYKDPHPNLRQAVCVWLLALLKYNIQRECIKQRFSSLHRAFMDYLADDSDVIQDIAAKGLSLIYVNINKEEKDSLVSTILSQFTQGRRSVQEVTPDTKLFEEGQLGKTPTSGNLTTYREICSLATELQKPDLVYYFMHLANHNAVWSSKKGAAFGFATIAKIANEELNKYLPSIVPRLYRYQYDPTPKIQQSMSSIWRAIIPSTSKAIEQYHEEILDDITNNLTHYEWRVRISCCNALADLLRANARLNLVKYAPELWKKLFRVMDDIHEGTRLAATNTTKILSKVCIRYCDSSHGKDGEEVLQAILPILLDIGVIHILSSVRSISLQTVSQLISRAGNLLKPSLVTLIPALLSAIGDSESPNLSYWSNVCGTATETRDVIDNIRASAAKGHYATETVTKCIQYIDAEVLKELMPKVIELIKSNVNFGTKITCLHFIILLSVHFKHEIQPYTGKILSTLLNGLLDCNSVVRKNNASTIGHIVGSMKESSLNKLFKTLNTWYMERDDTVKFAIGQTLQAINNYNQDVLRNYSNIVIPLTFLAMHEQKSQENENAIELWTDLWNDITPGTEAGIRQNVEPITNILRSSLESASWITKAQAANAINTLAQKLGNDLDATVRDTLLKVLIDGLRGRTWDGKERLLHALATLTCNSKQGLKENAEISADIVQVLYKETKKEALEYRRHALKAFGMVLHELDVDKFKEVYDIAQEIWTTLPKKDETESLISDERMKRTDDMVKLYEAIFETLGKAWPSCKETQDKYCIELITCCDKMFPLQSTTIQISMLVMLNLFLDKLSLLKTNYSELSIKDKEMLDSICNTLNNILTRSIGISYIRIRKEALNIVLSLGNKLRDTKNTELFDKMTFIMQKVLPELTKDNEPEIRTRLIDIKEMFKI

Target ORF information:

RefSeq Version	XM_012295987.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata proteasome-associated protein ECM29 homolog (LOC100881256), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012295987.1

ORF Insert Sequence:

ATGGACATAC AAAAGCAGGC TGAACTAATT CCAAGTGTAC TAAATGCTAT AGAAGGAAAA 
CCATTATCTC ATCAAGATAG TTTATTACTT ATAATTATGC CTGCATTGGG TCATATTAAT 
GTTCCCATGG ATCCAGATAA ACGAGCATCT CTTCTTGGTT TGCAAGATAA ACCTTATGTA 
TCAAAACAAT TAATCAATTT TATGCTTGAT GTTTTGTTAT TACCATATGG ATCAGTAGGA 
CAAACAGAAA ATCAACATCC AGGTCAAGCT ATTGATTGGT CACAGTTTCC TGTACCACCA 
GGATTAAGTG AATATGCATT TAAACGAGTA ATAGGTGAAA ATCCACCAAC AGCTGAACAA 
CTGGAACAAA CTAAATTAGG AATTGTCAAA TTTCTTAGTA GTGGATTCTT TCCCGATTCT 
GACATTCTCA TACATTTAAT AGTAGCAGCT GCTGATACCA GATTCAGTGT TGCTAATATG 
GCAGATTTAG AATTGAAAAA AATTGTTAAT ACATTAGATT GGTCTTCAAT GCAGTTAGCA 
GCTCCACTTT ATACTTTATT TTTGGGTACT AATGCTTTAG CTGCACAGAA AGAAGTTAAA 
TCAGAAATGA AACGATTACC AGCAAGTACG CGAATTCGTC TCAAATTGTT ACATTATCTT 
TGTCGTGTAA CTAAAACTGG TTTTATTATA CCACCTTGTA TTCAGGTAGT CTTTGATTCA 
CTGTACGGTA AAAATACAAA CACAAAATTA AAATCAATGG CATTGACATT TACATCAAAT 
ATTGTACAAC AGTGTAACCT AGCGCCACTA TCTCGTGTAG CTGGAGTTAT TTTAAATGGT 
ATGATAAAGT TAATTTCTGA AGGTGAACAT AATCATAAAG CAATGGCATA TACTGTTATT 
GGACAATTGG GACAAAGAAT ACCATCGTTA ATTAATAAGG ATGTAAATTT ATTACGTAAT 
TTATTCCAAA CACTGATATC GACGGAAGGT GATTTACGAA GAGCAGTACG AGATGCGTTA 
ATTACGGTCA CGTCTGCATT CGTACTTAGT AAAGACGATG AAACTAATAT ATCTTTATTG 
AATGAATTAT TGTCTACTTA TATCGAATCA CCAGAATATA ATGTCAGATT TGTAGCTATG 
CATTATGCTG CTACAGTATT CCCACCTGAT GATGCACCTT CTCGTTATCT TCTGTTGCTG 
GCATGTGGTG ATAATAAACA TGAAATAAGT AAAGAAGCCG CAAAAGTTCT GTACAGCACT 
TTGAATAAAA CTGAAAATGA TAAGCAAGAT AGTAACCAAA TTTTATTGCC AGACTTCTCA 
AAACTTGTTG TATATATTTA TTCTAAAATG CAAACGAGAA TGCCAACTTC AAGTACTGGT 
AAAGTGAATG TTGAAAGTAA GATACTTCCT TACAGTATTA CTGTATTTAC AGAGATAATT 
AATTATCTTC GTATCTGTTT AGCAAAAAGT GCTAATGCTA CCGTACAAAA TGAACCATTA 
CAGCATCCCT GTGAAAGTAC ACCTTTAATT GCACGTTATT TAGAAAATCT TTATAAAGAA 
GAACCATATA CAGTAAACAG TTATTTTGAT ATGGTTTTGA TCTTTAGTAA TGCCTCTGCA 
GATCAGACTT CTTTACAAGC TTTGTTAGAA CTGGTTAGTT CTATTCCACA ATATATAACG 
AAAATTTACG AAAAACAGTT GTCACGACTT CATAATTTAC TAACGTCTAC AAAAGTAGAT 
ATAAGACAAG TGGCTGCTAA AATTTATGGC GTAATAACTG CCCCACTTCC GGTTAATGAC 
TTCGAAACTC AAATTTCTGA AATTATAAAT ATTACAAATA AAAAGAATTT GGAAGCTCAA 
CACGGAGGAT TATTAGCTTT AACATATATG ATGGAAAGAC GATTATTAAT GTTTAGAAAG 
AATTCTACAA ATGATTCATT TAATTGGGAT CTTTATACTA ATGTTGTAAA AATAATATGT 
AATTTTCTTG AAAGTAGTAC AATTTTGTTA ATGGATGCTG CAATTCAGAG TATTGGTATT 
TTGGGAAAAA TTTGTGGATT ACCATTACCT AATGATGGTG AAGAACAACC CAATAAAAAA 
GCAATTGTGG AAATATTGTT TTCCACATTG ATTAACGCAA AATTAAATAC TAAGATTAAA 
GAAAAGGCTG CACTTTCACT AGGATATTTG TGCGTTGGAG AAAATTTTCC ACATACCTCA 
CAGATAATTG ACAAAATAAT TGGAATTGTT AAAGAAACGA AAGATATTGA AATTCATCTA 
ATTTTGGGAG AAGCATTAGT TTGCTGTGTT CAAGGTGAAG CCTCTCCAGA GAAAAGGGAT 
CCTTGGACAA CTTTACCGAA CGAACATGAA GTACCTTATA GTAACACCAG TACTGAACTA 
TTTATGCAGA CGTTAGATAG ATTACTTGGA ATGTATAAAG ATCCACACCC TAATTTAAGA 
CAGGCGGTCT GTGTATGGTT GTTAGCACTG TTAAAATATA ATATCCAAAG AGAATGCATT 
AAACAAAGAT TTTCGTCGTT ACACCGTGCA TTCATGGATT ACCTTGCAGA TGACAGTGAT 
GTAATACAGG ATATAGCAGC AAAAGGACTT AGTTTGATAT ACGTTAATAT TAACAAAGAA 
GAAAAAGATT CTTTAGTTTC TACTATATTA AGTCAATTTA CACAAGGGCG TAGAAGTGTA 
CAAGAAGTTA CACCCGACAC AAAATTGTTT GAAGAGGGTC AATTGGGAAA GACTCCAACA 
AGTGGCAACT TAACTACATA TAGAGAAATC TGTTCTTTGG CTACAGAATT ACAGAAACCA 
GATCTAGTAT ATTACTTTAT GCACTTAGCA AATCACAATG CAGTGTGGTC ATCTAAAAAA 
GGTGCTGCAT TTGGATTTGC AACGATTGCA AAAATAGCAA ATGAAGAATT AAATAAATAC 
TTGCCAAGCA TTGTACCACG CTTGTATAGA TATCAGTATG ATCCAACTCC TAAAATTCAA 
CAAAGCATGT CTAGTATTTG GCGTGCAATC ATTCCATCGA CAAGTAAAGC AATTGAACAG 
TATCATGAAG AAATATTAGA TGATATAACT AATAATTTAA CACATTACGA ATGGCGAGTT 
CGAATTAGTT GTTGCAATGC GCTTGCAGAT CTATTAAGAG CAAATGCTCG TTTAAATCTT 
GTAAAATATG CACCAGAATT GTGGAAAAAA TTGTTTCGAG TAATGGATGA TATTCACGAA 
GGTACGCGAT TAGCTGCTAC GAATACAACT AAAATACTTA GTAAAGTTTG CATCCGATAT 
TGTGATTCGT CTCATGGTAA AGACGGAGAA GAAGTACTGC AAGCAATATT GCCTATATTA 
TTAGATATTG GAGTTATTCA CATATTAAGC TCCGTAAGAT CGATATCGCT GCAAACAGTA 
TCTCAATTGA TTTCAAGAGC TGGTAATTTA CTTAAACCAT CTTTAGTCAC TTTAATTCCT 
GCATTGTTAA GTGCAATTGG CGATTCGGAA AGTCCTAATT TGTCGTACTG GAGTAATGTA 
TGTGGGACGG CAACAGAAAC ACGAGACGTT ATTGATAATA TTAGAGCTAG TGCTGCTAAA 
GGACATTATG CTACTGAAAC AGTAACGAAG TGCATACAGT ACATCGATGC AGAGGTTTTA 
AAAGAATTAA TGCCAAAGGT GATAGAATTA ATTAAATCTA ACGTGAATTT TGGAACGAAA 
ATTACTTGCT TACACTTCAT TATTCTTCTG AGTGTTCATT TTAAACATGA AATACAGCCT 
TACACTGGTA AAATTTTGTC AACTTTACTG AATGGATTAT TAGATTGCAA TTCTGTTGTT 
AGGAAAAATA ATGCAAGTAC TATTGGGCAT ATAGTTGGAT CAATGAAGGA ATCAAGTCTT 
AACAAGTTGT TTAAAACACT TAATACATGG TACATGGAAC GAGATGATAC GGTCAAGTTT 
GCAATTGGAC AAACATTACA AGCTATAAAC AATTATAATC AAGATGTACT AAGAAATTAT 
TCAAATATTG TTATACCTCT TACTTTTCTC GCAATGCATG AACAAAAATC ACAAGAGAAT 
GAAAATGCAA TTGAATTATG GACCGATCTA TGGAACGACA TAACTCCAGG AACTGAAGCT 
GGAATCAGGC AAAATGTTGA ACCAATAACA AACATTTTAC GTTCATCTTT AGAATCAGCT 
TCTTGGATTA CAAAAGCACA AGCAGCTAAT GCAATCAATA CTTTAGCGCA AAAATTGGGT 
AATGATCTTG ATGCAACTGT AAGAGACACT TTGTTAAAGG TGTTAATCGA TGGTTTACGC 
GGAAGAACTT GGGACGGCAA GGAACGATTG TTACATGCTC TTGCTACGCT GACATGTAAT 
AGCAAGCAAG GTCTTAAAGA GAATGCTGAG ATATCTGCAG ATATAGTGCA AGTGTTATAT 
AAAGAAACAA AAAAGGAGGC TTTAGAATAT CGCCGTCATG CTTTGAAAGC ATTTGGTATG 
GTTTTGCATG AATTAGACGT CGATAAGTTT AAGGAAGTGT ACGATATCGC TCAAGAAATT 
TGGACAACGT TGCCAAAGAA AGACGAAACC GAATCTCTAA TATCTGATGA GAGAATGAAG 
AGAACCGATG ACATGGTGAA ATTATACGAA GCCATTTTTG AAACACTTGG AAAAGCATGG 
CCATCTTGTA AAGAAACTCA AGATAAATAT TGTATAGAAC TTATAACATG TTGTGACAAA 
ATGTTTCCTC TTCAGTCTAC AACTATACAA ATATCAATGT TAGTAATGCT TAATTTATTT 
TTGGATAAAC TTTCTTTGCT CAAAACAAAT TACTCAGAAT TGTCAATCAA AGATAAAGAA 
ATGTTGGATT CAATTTGTAA CACGCTTAAT AATATATTAA CTCGTAGTAT AGGTATTTCG 
TACATCAGGA TAAGAAAAGA AGCCTTGAAC ATAGTTCTGT CCCTTGGTAA CAAGTTACGT 
GATACTAAAA ATACTGAACT ATTTGATAAA ATGACTTTTA TAATGCAGAA AGTCTTACCA 
GAACTAACAA AAGATAATGA ACCGGAAATT CGTACAAGAC TTATTGATAT CAAAGAGATG 
TTTAAAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100881256 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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