Col6a3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col6a3
Entrez Gene ID	12835
Full Name	collagen, type VI, alpha 3
Synonyms	AI507288,Col6a-3
General protein information	Preferred Names collagen, type VI, alpha 3 Names collagen alpha-3(VI) chain collagen alpha 3 chain type VI collagen alpha3(VI) procollagen, type VI, alpha 3 type VI collagen alpha 3 subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 D\|1 45.53 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001243008.1	NP_001229937.1	collagen alpha-3(VI) chain isoform 1 precursor
XM_030245438.1	XP_030101298.1	collagen alpha-3(VI) chain isoform X3
XM_030245451.1	XP_030101311.1	collagen alpha-3(VI) chain isoform X4
NM_001243009.1	NP_001229938.1	collagen alpha-3(VI) chain isoform 2 precursor
XM_030245436.1	XP_030101296.1	collagen alpha-3(VI) chain isoform X1
XM_030245437.1	XP_030101297.1	collagen alpha-3(VI) chain isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	COL6A3	XP_005205035.1
Canis lupus familiaris (dog)	COL6A3	NP_001096685.1
Mus musculus (house mouse)	Col6a3	NP_001229937.1
Xenopus tropicalis (tropical clawed frog)	col6a3	XP_002932067.2
Homo sapiens (human)	COL6A3	NP_004360.2
Pan troglodytes (chimpanzee)	COL6A3	XP_001153544.1
	COL6A3	XP_001084624.1
Rattus norvegicus (Norway rat)	Col6a3	XP_003750780.1
Gallus gallus (chicken)	COL6A3	NP_990865.1
Danio rerio (zebrafish)	col6a3	XP_002663415.3

Gene Ontology
Bibliography

Related articles in PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

Divergent functions of endotrophin on different cell populations in adipose tissue.
Zhao Y, Gu X, Zhang N, Kolonin MG, An Z, Sun K
American journal of physiology. Endocrinology and metabolism311(6)E952-E963(2016 Dec)

A collagen VI-dependent pathogenic mechanism for Hirschsprung's disease.
Soret R, Mennetrey M, Bergeron KF, Dariel A, Neunlist M, Grunder F, Faure C, Silversides DW, Pilon N,
The Journal of clinical investigation125(12)4483-96(2015 Dec)

Regulatory mechanisms of anthrax toxin receptor 1-dependent vascular and connective tissue homeostasis.
Besschetnova TY, Ichimura T, Katebi N, St Croix B, Bonventre JV, Olsen BR
Matrix biology : journal of the International Society for Matrix Biology4256-73(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of endotrophin caused upregulation of adipogenic genes in the white adipose tissue(WAT) of endotrophin transgenic mice. The mice exhibited inhibited lipolysis and accelerated hypertrophy and hyperplasia in WAT.
Title: Divergent functions of endotrophin on different cell populations in adipose tissue.

Data indicate that overexpression of endotrophin (COL6alpha3) locally in adipose tissue promotes metabolic dysfunction under a high-fat diet (HFD) challenge.
Title: Endotrophin triggers adipose tissue fibrosis and metabolic dysfunction.

Data show that the TFIID subunit TAF4 is essential to restrain the growth promoting properties of collagen 6A3 (Col6a3).
Title: TAF4 inactivation reveals the 3 dimensional growth promoting activities of collagen 6A3.

mice lacking normal alpha3(VI) collagen chains display mild musculoskeletal phenotypes similar to mice deficient in the alpha1(VI) collagen alpha chain, suggesting that alpha3(VI) collagen does not elicit essential functions in normal growth and development
Title: COL6A3 protein deficiency in mice leads to muscle and tendon defects similar to human collagen VI congenital muscular dystrophy.

analysis of collagen VI chains alpha4, alpha5, and alpha6with high homology to the alpha3 chain
Title: Three novel collagen VI chains with high homology to the alpha3 chain.

The following Col6a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col6a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84999	XM_030245451.1 Latest version!	Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu03629	NM_001243008.1 Latest version!	Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu03632	NM_001243009.1 Latest version!	Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu84996	XM_030245436.1 Latest version!	Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu84997	XM_030245437.1 Latest version!	Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu84998	XM_030245438.1 Latest version!	Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col6a3 ( XM_030245451.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101311.1 Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu84999
Clone ID Related Accession (Same CDS sequence)	XM_030245451.1
Accession Version	XM_030245451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-3(VI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAAC ACCGGCATCT GCCCCTAGTG GCTGTCTTTA GCCTTCTCCT CTCAGGCATT 
GCCACGACTC ATGCCCAACA GCATGGAGAG AGCAAGCGGG ACATTCTTTT CCTCTTTGAC 
GGCTCAGTCA ATGTCTTGGG TCAGTTCCCT GCTGTCAGAG ACTTTCTCTA CAGGATCATT 
GAGGAACTTG ATGTGAAGCC GGATGGGACC CGGGTAGCAA TAGCTCAGTT CAGTGATGAC 
GTCAGGCTGG AGTCCCGCTT CAGTGAGCAC CAGACGAAAG CTGAAATCCT TAATCTCGTG 
AAGAAGATGA AGCTAAAAAC AGGCAAAGCC CTCAACCTGG GCTACGCCCT GGACTACGCG 
CTTAGGAACA TCTTTGTGAG GTCGGCTGGC AGCCGCATCG AGGATAACGT GCAGCAGTTC 
CTGGTGCTGC TGGTTGCTGG AAGGTCGTCA GATGCTGTGG CCGGGCCTGC AAGCTCCCTG 
AAGCAGAGAG GAGTGGTACC TTTCATCTTT CAAGCCAAGA ACGCCAACCC CAGTGAGCTG 
GAGCAGATTG TGCTGTCGCC TGCATTCATC CTGGCTGCAG AGTCACTCCC TAAGATCGGA 
GACCTCCAAT CACAGATTGT GAGCCTTCTG AAAGCAGAAC AGGGTTCAGG CCCTGTATCA 
GGTGAAAAGG ATGTGGTGTT TCTCATTGAT GGCTCTGAGG GTGTCCGCAG TGGCTTCCCC 
CTGCTAAAGG ACTTTGTGCA GAGGGTTGTG GAGAGCTTGG ATGTGGGTCC CGACCGTGTG 
CGTGTGGCAC TGGTGCAGTA CAGTGACCGG ACCAGGCCGG AGTTCTACCT GAATTCCCAC 
ATGGACCAGC AGGGTGTCAT CAGCGCCATC CGCAGGCTGA CACTGCTGGG CGGCCCAACC 
CCCAACACAG GGGCGGCACT GGAGTTCGTG TTAAGGAACA TTCTGACCAG TTCTACTGGG 
AGCAGGATAG CAGAAGGTGT TCCTCAGCTC CTGATTGTCC TCACAGCAGA GCCATCAGGG 
GATGACGTGC GAGGCCCTTC AGTAGTCCTG AAGCAGGGCG GGGCTGTGCC CATTGGCATT 
GGCATTGGGA ATGCTGACAT CTCTGAAATG CAGACCATCT CCTTCATCCC TGACTTTGCT 
GTGGCCATCC CCACCTTCCG GGAGCTTGGG ACGATACAGC AGGTCATCTC TGAGAGGGTG 
ATCCAGCTTA ATCGTGAGGA GCTGAGCTCG TTGAAACCCA TTTTGACGCC CTCAACAGGT 
GCAGGTGTTG GCAGCAAGAA AGATGTGGTC TTTCTCATCG ATGGATCCCG AAATGCTGGC 
CCTGAGTTCC AGTACATCCG TACCCTGATT GAGAGGATAG TCGAGTACCT GGACATAGGC 
TTTGACACCA CCCGGGTAGC CGTCATCCAG TTCAGTGAGG ACTCCAAGAT GGAGTTTCCT 
CTGAATGCCC ACTTCAGCAA GGATGAAGTA CAGAACGCTG TGCGCAGGCT GCGGCCCAAG 
GGTGGGAGTC AGGTCTACAT CGGAAATGCC CTGGAGTACG TGCTGAAGAA CATCTTCCAG 
AGGCCACTGG GGAGCCGGAT AGAAGAGGGC GTTCCGCAGT TCCTAGTCCT CATTTCGTCT 
GGAAAGTCTG ATGATGAGGT GGACGACTCA GCCGTGGAAC TCAAGCAGTT CGGTGTGGCC 
CCTCTCACCA TAGCCAGACA CACAGACCAA GAGGAGCTGG TGAAGATCTC CCTAAGTCCT 
GAGTACGTGT ACTCCGTGAG CACCTTCAGG GAGCTGCCCA GACTGGAGCA GAAGCTGCTG 
ACGCCCATCA CCACTCTAAC CTCACAGCAG ATCCATCAGA TCCTGGCCAG TACCCGATAT 
CCCCCTTCAG TGGTTGAAAG CGATGCGGCT GACATCGTCT TTCTGATTGA CAGCTCTGAT 
GCCGTCAAGC CCGATGGCAT CGCTCATATC CGAGACTTTG TCAGCAGGAT CGTTCGCAGA 
CTCAACATTG GTCCCAGTAA AGTGAGGATT GGGGTCGTGC AGTTTAGCAA TGATGTCTTC 
CCTGAGTTCT ACCTGAAGAC CCACAAGTCC CAGAGCAGTG TGCTTGAAGC CATACGGCGT 
CTGAGGTTCA AAGGAGGGTC TCCCCTAAAC ACTGGCAGAG CCCTGGAGTT TGTGGCAAGA 
AACCTCTTCG TGAAGTCTGC TGGGAGCCGG ATAGAAGATG GGGTCCCTCA ACACCTGGTC 
TTATTCCTGG GTGGGAAGTC TCAGGACGAT GTGGCCAGGC ATGCCCAAGT CATAAGCTCA 
TCGGGGATTG TGAGCTTAGG AATAGGAGAT CGGAACATTG ATAGGACGGA CTTGCAAACC 
ATCACCAATG ACCCCAGGCT GGTCTTCACA GTTCGGGAAT TCAGAGAGCT GCCCAACATA 
GAAGAGAGGG TCATGCTTTC CTTTGGACCC TCTGGGGCTA CTCCTCAGCC TCCTGGGGTA 
GATTTACCTT CTCCTTCCCG GCCAGAGAAG AAGAAGGCCG ACATTGTGTT CCTGCTGGAT 
GGTTCCATCA ATTTTAGGAG GGACAGTTTC CAGGAAGTGC TCCGTTTTGC ATCTGAAATC 
GTGGACACGG TCTATGAAGA CGGAGATTCC ATCAGAGTGG GACTGGTCCA GTACAACTCA 
GACCCCACGG ATGAATTCTT CCTGAGGGAC TTTTCTACCA AAAGGCAGAT TATCGATGCC 
ATCAACAAAG TCGTCTATAA AGGAGGGAGG CATGCCAACA CCAGGGTGGG CATCGAGCAC 
CTGCTGCGGA ATCACTTTGT GCCGGAGGCT GGTAGCCGCC TGGATGAGCG GGTTCCTCAG 
ATCGCCTTCG TGATCACCGG AGGGAAGTCA GTGGAGGATG CTCAGGATGT GAGCCTGGCT 
CTCACCCAGA AAGGTGTCAA GGTGTTTGCC GTTGGCGTGA GGAACATCGA CTCTGAGGAG 
GTGGGCAAGA TAGCCTCCAA CAGTGCCACA GCCTTCCGGG TGGGCAGCGT GCAGGAACTG 
TCCGAACTGA GTGAGACAGT GCTCGAGACT CTGCATGATG CAATGCATGA GACCCTCTGC 
CCTGGTGTGA CCGATGTCTC CAAAGCCTGC AATCTGGAAG TCATTCTGGG GTTTGATGGA 
TCTCGAGATC AGAATGTGTT TGTGAGTCAG AAGGGCCTTG AGTCCAAGGT GGACATCATC 
TTGAACAGAA TCAGCCAGAT ACAAAGGATC AGCTGCAGTG GCAACCAGCT GCCCACTGTG 
AGGGTATCCG TGATGGCGAA CACGCCCTCT GGGCCAGTGG AGGCCTTTGA CTTTGCCGAG 
TATCAGCCGG AGCTGTTTGA GAAGTTTCGC AACATGCGCA GTCAGCGCCC ATACGTCCTC 
ACAGCTGACA CGCTGAAGTT GTACCAGAAC AAGTTCAGGC AGTCGTCGCC TGATACTGTG 
AAGGTAGTCA TTCACTTCAC GGATGGAGCA GATGGAGACA TGGCTGACTT ATACAGAGCA 
TCTGAGGAAC TCCGGCAAGC AGGTGCCCAA GCCCTGATCC TGGTGGGTCT TGAACGTGTG 
GCTAACCTGG AGCGGCTGAT GCATCTGGAG TTCGGGCGGG GCTTCATGTA TGACAGGCCC 
CTGAGGCTCA ACCTGCTTGA CTTAGACTAT GAACTGGCAG AGCAGCTTGA CAATATTGCT 
GAGAAAGCTT GCTGTGGGGT TCCATGCAAG TGCTCTGGAG AGAGGGGAGA CAGAGGGCCC 
ATTGGCAGCA TTGGGCCAAA GGGTATCTCC GGGGAAGATG GCTACCGAGG CTACCCGGGA 
GACGAAGGCG GACCTGGTGA GCGAGGTCCA CCTGGTGTGA ATGGCACACA AGGTTTCCAG 
GGCTGTCCAG GCCAGAGGGG AGTGAAGGGT TCTCGAGGAT TCCCAGGAGA GAAGGGTGAA 
TTGGGAGAAA TCGGCTTGGA TGGTCTGGAT GGTGAAGAGG GAGACAAGGG GCTGCCTGGT 
TCTTCTGGAG AAAAGGGGAG TCCTGGAAGA AGGGGTGACA AAGGACCCAA AGGAGACAAA 
GGCGAGAGAG GAGATGTCGG GATTCGAGGT GACCCGGGTG ACTCAGGACG GGACAGCCAG 
CAGAGAGGAC CCAAAGGAGA GACGGGAGAC ATCGGCCCCA TGGGTCTCCC AGGCAGAGAT 
GGCATCCCTG GCAGCCCAGG AGACCCTGGG AAGGACGGTG GCTCTGGCCG AAGGGGCCCT 
GCAGGAGCTA AGGGCAACAG AGGTGGTCCT GGCCAGCCAG GCTTCGAAGG CGAGCAGGGT 
ACCAGAGGCT CACAGGGCCC ACCTGGTCCC ATTGGTCCCC CAGGCCTGAT TGGGGAACAA 
GGAATTCCCG GACCTCGAGG AGGCGGAGGT ACGGCTGGGG CTCCTGGAGA ACGTGGCAGA 
ACTGGTCCTC TGGGGAGGAA GGGTGAGCCA GGAGAGCCAG GGCCGAAGGG AAGCATCGGG 
AACCGTGGAC CCCGAGGAGA GACGGGAGAT GACGGGAGAG ATGGGGTTGG CAGTGAAGGA 
CGCAGAGGCA AAAAAGGAGA AAGAGGCTTC CCTGGGTATC CGGGACCAAA GGGCACCCCT 
GGTGAGCCAG GGGCAGATGG ACCACCAGGA CCCAAAGGCA TCAGAGGTCG AAGGGGAAAT 
TCAGGACCTC CAGGGGCAAC TGGACAAAAG GGAGACCCTG GTTACCCAGG ACCATCTGGT 
CACAAGGGCA ACCGAGGCGA CTCCGTTGAT CAATGCGCCC TCATCCAGAG CATCAGAGAC 
AAATGCCCTT GCTGCTACGG GCCCCTGGAG TGCCCTGTAT TCCCAACAGA ACTCGCCTTT 
GCCCTGGATA CCTCTGAGGG GGTGACTCAG GACACCTTCA GCCGGATGCG AGAGGTGCTT 
CTGGGCATTG TGGGCGATCT GACCATTGCA GAGAGCAACT GTCCGCGGGG TGCCCGCGTG 
GCTGTGGTCA CCTATAACAA TGAGGTGACT ACAGAGATCC GGTTTGCTGA CTCCAAGAAG 
AAGTCTGCCC TCTTAGACAG TATTCAGAAC CTCCAAGTGG CCTTGACCTC TAAGCAGCAG 
AGCCTGGAGA CCGCAATGTC ATTCGTGGCC AGGAATACAT TCAAACGTGT GAGGAGTGGC 
TTCCTGATGA GGAAAGTGGC TGTTTTCTTC AGCAACAAGC CCACCAGGGC ATCCCCCCAG 
CTCCGAGAGG CCGTACTCAA GCTTTCCGAT GCGGGGATCA CACCCTTGTT CCTTACAAGC 
CAGGAGGACC GACAGCTCAT CAACGCTTTG CAGATCAATA ACACAGCAGT GGGACATGCC 
CTGGTTCTGC CTGCAAGGAG GGATCTCACA GACTTCCTGA AGAATGTCCT TACCTGCCAT 
GTTTGCTTGG ACATTTGCAA TATCGACCCA TCCTGTGGCT TCGGCAGCTG GAGGCCTTCC 
TTCAGGGACC GGAGGGCAGC AGGCAGTGAT GTGGACATAG ACCTGGCTTT CATCTTGGAC 
AGCTCAGAGG CTACCACACT GTTCCAGTTC AATGAGATGA AGAAATACAT AGGCTATGTG 
ATCAGACAGC TGGACCTGAG CCCAGACCCG AAGGCTTCCC AACACTTTGC CAGGGTGGCT 
GTTGTGCAGC AGTCAACCTA CGAGTCTGTG GACAATGCCA GTGTGCCGCC TGTGAAGGTG 
GAATTCTCAC TGACAGACTA TGGTGCCAAG GAAAAGCTGT TGGATTTCCT TAGCCGGAGG 
ATGACCCAAC TTCAGGGAAC CATGGGCCTG GGCAATGCCA TTGAATACAC TATAGAGAAC 
ATCTTTGAAA GTGCTCCAAA CCCACGGGAC CTCAAAATTA TGGTGCTGAT GTTGACTGGT 
GACATGCAGA GGCAGCAGCT GGAGGAGGCC CAGAGAGCCA TCCTCCAGGC CAAGTGCAAG 
GGTTACTTCT TTGTGGTTCT GGGCATTGGC AGGAAGGTGA ATATCAAGGA GGTCTACAGC 
TTTGCCAGTG AGCCCAATGA TGTCTTCTTC AAATTTGTGG ACAAATCAAC TGAGCTCAAC 
GAGGAGCCTC TGATGCGCTT TGGGAGGCTG CTCCCATCCT TTGTCAGCAG TGAAAATGCA 
TTTTACCTGC CTCCAGATCT CAGGAAACAG TGTGATTGGT TCCAAGGGGA CCAACCAGTG 
AAGAATGGCG TGAAGTTTGG TCACAAACAG ATAAACCCTC CACATACTGC TAATTCAAGT 
CTTACATCCA AAGTTGTGAC CACAATGAAG CCAGTGACAA CGACCAAACC AACAGCAATT 
GTAAACCTGC CACCTGCTAA GCCAGCCCCA GCGAGGCCAG CCCCTGCTCA GCCAGTTCTT 
GCTAAGCCAG ACCCCGCAAA ACCAGCCCAA GCAAGGCCAG CCCCTGCTAA GCCAGCTTCT 
GCTAAGTTGG TGCCTCCTCA GCCAGTCCAT GTTCAGCCAG CCCCTGCCCA GACAGCTTCT 
GTAAGACCAG CCCCCGCTAA GCCAGCCCCT CCTCAGCCAG CTGCTGCTAA GCCAGTCCCT 
GCTAAGCCAG CAGTCCCTGC TCAGCCAGCC CCTCCTCAGC CAGCTGCTGC TAAGCCAGTC 
CCTGCTAAGC CAGCAGTCCC TGCTCAGCCA GCCCCTCCTC AGCCAGCTGC TGCTAAGCCA 
GTCCCTGCTA AGCCAGCAGT CCCTGCTCAG CCAGCCGCTG CTCAACCAAT GCCTGCTCAG 
CCAGTCCTTA CTAAATCAGC CGCTGTGAAA CCAGCCTCTG CCAACAAGCC TGTGGCAGCC 
AAACCTGTGG CCACGAACAC AGCCACAGCC ACAGCCAGAC CTGCATTAGC AGCGAAGCCT 
GCTGCAGCGA AGCCTGCAGC AACGAGACCC CTTGCTGCTG CCATAAGGCC AGTTGCTACC 
AAGCCAGAGG CCCCCAGGCA ACAGGCCAAA CCAGCTGCCA CCAAGCCAGC CACTACTAAG 
CCCTTGGCGA GAGTGTCCCG AGAAGTCCAG GTCTCTGAAG TCACGGAGAA CAGTGCCAGA 
CTCCACTGGG AGCGGCCCGA GCCTTCCAGC TCCTTTTTTT ATGACCTCAC TGTAACCTCG 
GCCCACGACC AGTCCTTGGT TCTGAGACAA AACCTCACTG TCACTGACCG TGTCATCGGA 
GGCCTACTGG CTGGACAGCT GTACCATGTC GTAGTGGTCT CCTACCTGCA GTCTCAGGTC 
AGGGCCATCT ACCAAGGAAG TTTCAATACG AAGAAAACCC AGCCTCCACC TCTTCAGGCA 
GCACACCGAG CATCCAGTTC AACCATCAAC CTCATGGTGA ACACAGAACC ATTGTTTCTC 
ACTAAAACAG ATATATGTAA GCTGTCCAGA GATGCTGGGA CTTGTGTGGA CTTCAAGTTA 
CTATGGCACT ATGACCTAGA GAGCAAAAGT TGCAAGAGAT TCTGGTATGG AGGTTGTGGA 
GGCAACGAGA ACAGATTCCA CTCCCAGGAA GAATGTGAAA AGATGTGTAG TCCTGAGTTA 
ACAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101311.1
CDS	276..7724
Translation	MRKHRHLPLVAVFSLLLSGIATTHAQQHGESKRDILFLFDGSVNVLGQFPAVRDFLYRIIEELDVKPDGTRVAIAQFSDDVRLESRFSEHQTKAEILNLVKKMKLKTGKALNLGYALDYALRNIFVRSAGSRIEDNVQQFLVLLVAGRSSDAVAGPASSLKQRGVVPFIFQAKNANPSELEQIVLSPAFILAAESLPKIGDLQSQIVSLLKAEQGSGPVSGEKDVVFLIDGSEGVRSGFPLLKDFVQRVVESLDVGPDRVRVALVQYSDRTRPEFYLNSHMDQQGVISAIRRLTLLGGPTPNTGAALEFVLRNILTSSTGSRIAEGVPQLLIVLTAEPSGDDVRGPSVVLKQGGAVPIGIGIGNADISEMQTISFIPDFAVAIPTFRELGTIQQVISERVIQLNREELSSLKPILTPSTGAGVGSKKDVVFLIDGSRNAGPEFQYIRTLIERIVEYLDIGFDTTRVAVIQFSEDSKMEFPLNAHFSKDEVQNAVRRLRPKGGSQVYIGNALEYVLKNIFQRPLGSRIEEGVPQFLVLISSGKSDDEVDDSAVELKQFGVAPLTIARHTDQEELVKISLSPEYVYSVSTFRELPRLEQKLLTPITTLTSQQIHQILASTRYPPSVVESDAADIVFLIDSSDAVKPDGIAHIRDFVSRIVRRLNIGPSKVRIGVVQFSNDVFPEFYLKTHKSQSSVLEAIRRLRFKGGSPLNTGRALEFVARNLFVKSAGSRIEDGVPQHLVLFLGGKSQDDVARHAQVISSSGIVSLGIGDRNIDRTDLQTITNDPRLVFTVREFRELPNIEERVMLSFGPSGATPQPPGVDLPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFASEIVDTVYEDGDSIRVGLVQYNSDPTDEFFLRDFSTKRQIIDAINKVVYKGGRHANTRVGIEHLLRNHFVPEAGSRLDERVPQIAFVITGGKSVEDAQDVSLALTQKGVKVFAVGVRNIDSEEVGKIASNSATAFRVGSVQELSELSETVLETLHDAMHETLCPGVTDVSKACNLEVILGFDGSRDQNVFVSQKGLESKVDIILNRISQIQRISCSGNQLPTVRVSVMANTPSGPVEAFDFAEYQPELFEKFRNMRSQRPYVLTADTLKLYQNKFRQSSPDTVKVVIHFTDGADGDMADLYRASEELRQAGAQALILVGLERVANLERLMHLEFGRGFMYDRPLRLNLLDLDYELAEQLDNIAEKACCGVPCKCSGERGDRGPIGSIGPKGISGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEEGDKGLPGSSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGDSGRDSQQRGPKGETGDIGPMGLPGRDGIPGSPGDPGKDGGSGRRGPAGAKGNRGGPGQPGFEGEQGTRGSQGPPGPIGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGEPGPKGSIGNRGPRGETGDDGRDGVGSEGRRGKKGERGFPGYPGPKGTPGEPGADGPPGPKGIRGRRGNSGPPGATGQKGDPGYPGPSGHKGNRGDSVDQCALIQSIRDKCPCCYGPLECPVFPTELAFALDTSEGVTQDTFSRMREVLLGIVGDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSALLDSIQNLQVALTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRASPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPARRDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDLAFILDSSEATTLFQFNEMKKYIGYVIRQLDLSPDPKASQHFARVAVVQQSTYESVDNASVPPVKVEFSLTDYGAKEKLLDFLSRRMTQLQGTMGLGNAIEYTIENIFESAPNPRDLKIMVLMLTGDMQRQQLEEAQRAILQAKCKGYFFVVLGIGRKVNIKEVYSFASEPNDVFFKFVDKSTELNEEPLMRFGRLLPSFVSSENAFYLPPDLRKQCDWFQGDQPVKNGVKFGHKQINPPHTANSSLTSKVVTTMKPVTTTKPTAIVNLPPAKPAPARPAPAQPVLAKPDPAKPAQARPAPAKPASAKLVPPQPVHVQPAPAQTASVRPAPAKPAPPQPAAAKPVPAKPAVPAQPAPPQPAAAKPVPAKPAVPAQPAPPQPAAAKPVPAKPAVPAQPAAAQPMPAQPVLTKSAAVKPASANKPVAAKPVATNTATATARPALAAKPAAAKPAATRPLAAAIRPVATKPEAPRQQAKPAATKPATTKPLARVSREVQVSEVTENSARLHWERPEPSSSFFYDLTVTSAHDQSLVLRQNLTVTDRVIGGLLAGQLYHVVVVSYLQSQVRAIYQGSFNTKKTQPPPLQAAHRASSSTINLMVNTEPLFLTKTDICKLSRDAGTCVDFKLLWHYDLESKSCKRFWYGGCGGNENRFHSQEECEKMCSPELTV

Target ORF information:

RefSeq Version	XM_030245451.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245451.1

ORF Insert Sequence:

ATGAGGAAAC ACCGGCATCT GCCCCTAGTG GCTGTCTTTA GCCTTCTCCT CTCAGGCATT 
GCCACGACTC ATGCCCAACA GCATGGAGAG AGCAAGCGGG ACATTCTTTT CCTCTTTGAC 
GGCTCAGTCA ATGTCTTGGG TCAGTTCCCT GCTGTCAGAG ACTTTCTCTA CAGGATCATT 
GAGGAACTTG ATGTGAAGCC GGATGGGACC CGGGTAGCAA TAGCTCAGTT CAGTGATGAC 
GTCAGGCTGG AGTCCCGCTT CAGTGAGCAC CAGACGAAAG CTGAAATCCT TAATCTCGTG 
AAGAAGATGA AGCTAAAAAC AGGCAAAGCC CTCAACCTGG GCTACGCCCT GGACTACGCG 
CTTAGGAACA TCTTTGTGAG GTCGGCTGGC AGCCGCATCG AGGATAACGT GCAGCAGTTC 
CTGGTGCTGC TGGTTGCTGG AAGGTCGTCA GATGCTGTGG CCGGGCCTGC AAGCTCCCTG 
AAGCAGAGAG GAGTGGTACC TTTCATCTTT CAAGCCAAGA ACGCCAACCC CAGTGAGCTG 
GAGCAGATTG TGCTGTCGCC TGCATTCATC CTGGCTGCAG AGTCACTCCC TAAGATCGGA 
GACCTCCAAT CACAGATTGT GAGCCTTCTG AAAGCAGAAC AGGGTTCAGG CCCTGTATCA 
GGTGAAAAGG ATGTGGTGTT TCTCATTGAT GGCTCTGAGG GTGTCCGCAG TGGCTTCCCC 
CTGCTAAAGG ACTTTGTGCA GAGGGTTGTG GAGAGCTTGG ATGTGGGTCC CGACCGTGTG 
CGTGTGGCAC TGGTGCAGTA CAGTGACCGG ACCAGGCCGG AGTTCTACCT GAATTCCCAC 
ATGGACCAGC AGGGTGTCAT CAGCGCCATC CGCAGGCTGA CACTGCTGGG CGGCCCAACC 
CCCAACACAG GGGCGGCACT GGAGTTCGTG TTAAGGAACA TTCTGACCAG TTCTACTGGG 
AGCAGGATAG CAGAAGGTGT TCCTCAGCTC CTGATTGTCC TCACAGCAGA GCCATCAGGG 
GATGACGTGC GAGGCCCTTC AGTAGTCCTG AAGCAGGGCG GGGCTGTGCC CATTGGCATT 
GGCATTGGGA ATGCTGACAT CTCTGAAATG CAGACCATCT CCTTCATCCC TGACTTTGCT 
GTGGCCATCC CCACCTTCCG GGAGCTTGGG ACGATACAGC AGGTCATCTC TGAGAGGGTG 
ATCCAGCTTA ATCGTGAGGA GCTGAGCTCG TTGAAACCCA TTTTGACGCC CTCAACAGGT 
GCAGGTGTTG GCAGCAAGAA AGATGTGGTC TTTCTCATCG ATGGATCCCG AAATGCTGGC 
CCTGAGTTCC AGTACATCCG TACCCTGATT GAGAGGATAG TCGAGTACCT GGACATAGGC 
TTTGACACCA CCCGGGTAGC CGTCATCCAG TTCAGTGAGG ACTCCAAGAT GGAGTTTCCT 
CTGAATGCCC ACTTCAGCAA GGATGAAGTA CAGAACGCTG TGCGCAGGCT GCGGCCCAAG 
GGTGGGAGTC AGGTCTACAT CGGAAATGCC CTGGAGTACG TGCTGAAGAA CATCTTCCAG 
AGGCCACTGG GGAGCCGGAT AGAAGAGGGC GTTCCGCAGT TCCTAGTCCT CATTTCGTCT 
GGAAAGTCTG ATGATGAGGT GGACGACTCA GCCGTGGAAC TCAAGCAGTT CGGTGTGGCC 
CCTCTCACCA TAGCCAGACA CACAGACCAA GAGGAGCTGG TGAAGATCTC CCTAAGTCCT 
GAGTACGTGT ACTCCGTGAG CACCTTCAGG GAGCTGCCCA GACTGGAGCA GAAGCTGCTG 
ACGCCCATCA CCACTCTAAC CTCACAGCAG ATCCATCAGA TCCTGGCCAG TACCCGATAT 
CCCCCTTCAG TGGTTGAAAG CGATGCGGCT GACATCGTCT TTCTGATTGA CAGCTCTGAT 
GCCGTCAAGC CCGATGGCAT CGCTCATATC CGAGACTTTG TCAGCAGGAT CGTTCGCAGA 
CTCAACATTG GTCCCAGTAA AGTGAGGATT GGGGTCGTGC AGTTTAGCAA TGATGTCTTC 
CCTGAGTTCT ACCTGAAGAC CCACAAGTCC CAGAGCAGTG TGCTTGAAGC CATACGGCGT 
CTGAGGTTCA AAGGAGGGTC TCCCCTAAAC ACTGGCAGAG CCCTGGAGTT TGTGGCAAGA 
AACCTCTTCG TGAAGTCTGC TGGGAGCCGG ATAGAAGATG GGGTCCCTCA ACACCTGGTC 
TTATTCCTGG GTGGGAAGTC TCAGGACGAT GTGGCCAGGC ATGCCCAAGT CATAAGCTCA 
TCGGGGATTG TGAGCTTAGG AATAGGAGAT CGGAACATTG ATAGGACGGA CTTGCAAACC 
ATCACCAATG ACCCCAGGCT GGTCTTCACA GTTCGGGAAT TCAGAGAGCT GCCCAACATA 
GAAGAGAGGG TCATGCTTTC CTTTGGACCC TCTGGGGCTA CTCCTCAGCC TCCTGGGGTA 
GATTTACCTT CTCCTTCCCG GCCAGAGAAG AAGAAGGCCG ACATTGTGTT CCTGCTGGAT 
GGTTCCATCA ATTTTAGGAG GGACAGTTTC CAGGAAGTGC TCCGTTTTGC ATCTGAAATC 
GTGGACACGG TCTATGAAGA CGGAGATTCC ATCAGAGTGG GACTGGTCCA GTACAACTCA 
GACCCCACGG ATGAATTCTT CCTGAGGGAC TTTTCTACCA AAAGGCAGAT TATCGATGCC 
ATCAACAAAG TCGTCTATAA AGGAGGGAGG CATGCCAACA CCAGGGTGGG CATCGAGCAC 
CTGCTGCGGA ATCACTTTGT GCCGGAGGCT GGTAGCCGCC TGGATGAGCG GGTTCCTCAG 
ATCGCCTTCG TGATCACCGG AGGGAAGTCA GTGGAGGATG CTCAGGATGT GAGCCTGGCT 
CTCACCCAGA AAGGTGTCAA GGTGTTTGCC GTTGGCGTGA GGAACATCGA CTCTGAGGAG 
GTGGGCAAGA TAGCCTCCAA CAGTGCCACA GCCTTCCGGG TGGGCAGCGT GCAGGAACTG 
TCCGAACTGA GTGAGACAGT GCTCGAGACT CTGCATGATG CAATGCATGA GACCCTCTGC 
CCTGGTGTGA CCGATGTCTC CAAAGCCTGC AATCTGGAAG TCATTCTGGG GTTTGATGGA 
TCTCGAGATC AGAATGTGTT TGTGAGTCAG AAGGGCCTTG AGTCCAAGGT GGACATCATC 
TTGAACAGAA TCAGCCAGAT ACAAAGGATC AGCTGCAGTG GCAACCAGCT GCCCACTGTG 
AGGGTATCCG TGATGGCGAA CACGCCCTCT GGGCCAGTGG AGGCCTTTGA CTTTGCCGAG 
TATCAGCCGG AGCTGTTTGA GAAGTTTCGC AACATGCGCA GTCAGCGCCC ATACGTCCTC 
ACAGCTGACA CGCTGAAGTT GTACCAGAAC AAGTTCAGGC AGTCGTCGCC TGATACTGTG 
AAGGTAGTCA TTCACTTCAC GGATGGAGCA GATGGAGACA TGGCTGACTT ATACAGAGCA 
TCTGAGGAAC TCCGGCAAGC AGGTGCCCAA GCCCTGATCC TGGTGGGTCT TGAACGTGTG 
GCTAACCTGG AGCGGCTGAT GCATCTGGAG TTCGGGCGGG GCTTCATGTA TGACAGGCCC 
CTGAGGCTCA ACCTGCTTGA CTTAGACTAT GAACTGGCAG AGCAGCTTGA CAATATTGCT 
GAGAAAGCTT GCTGTGGGGT TCCATGCAAG TGCTCTGGAG AGAGGGGAGA CAGAGGGCCC 
ATTGGCAGCA TTGGGCCAAA GGGTATCTCC GGGGAAGATG GCTACCGAGG CTACCCGGGA 
GACGAAGGCG GACCTGGTGA GCGAGGTCCA CCTGGTGTGA ATGGCACACA AGGTTTCCAG 
GGCTGTCCAG GCCAGAGGGG AGTGAAGGGT TCTCGAGGAT TCCCAGGAGA GAAGGGTGAA 
TTGGGAGAAA TCGGCTTGGA TGGTCTGGAT GGTGAAGAGG GAGACAAGGG GCTGCCTGGT 
TCTTCTGGAG AAAAGGGGAG TCCTGGAAGA AGGGGTGACA AAGGACCCAA AGGAGACAAA 
GGCGAGAGAG GAGATGTCGG GATTCGAGGT GACCCGGGTG ACTCAGGACG GGACAGCCAG 
CAGAGAGGAC CCAAAGGAGA GACGGGAGAC ATCGGCCCCA TGGGTCTCCC AGGCAGAGAT 
GGCATCCCTG GCAGCCCAGG AGACCCTGGG AAGGACGGTG GCTCTGGCCG AAGGGGCCCT 
GCAGGAGCTA AGGGCAACAG AGGTGGTCCT GGCCAGCCAG GCTTCGAAGG CGAGCAGGGT 
ACCAGAGGCT CACAGGGCCC ACCTGGTCCC ATTGGTCCCC CAGGCCTGAT TGGGGAACAA 
GGAATTCCCG GACCTCGAGG AGGCGGAGGT ACGGCTGGGG CTCCTGGAGA ACGTGGCAGA 
ACTGGTCCTC TGGGGAGGAA GGGTGAGCCA GGAGAGCCAG GGCCGAAGGG AAGCATCGGG 
AACCGTGGAC CCCGAGGAGA GACGGGAGAT GACGGGAGAG ATGGGGTTGG CAGTGAAGGA 
CGCAGAGGCA AAAAAGGAGA AAGAGGCTTC CCTGGGTATC CGGGACCAAA GGGCACCCCT 
GGTGAGCCAG GGGCAGATGG ACCACCAGGA CCCAAAGGCA TCAGAGGTCG AAGGGGAAAT 
TCAGGACCTC CAGGGGCAAC TGGACAAAAG GGAGACCCTG GTTACCCAGG ACCATCTGGT 
CACAAGGGCA ACCGAGGCGA CTCCGTTGAT CAATGCGCCC TCATCCAGAG CATCAGAGAC 
AAATGCCCTT GCTGCTACGG GCCCCTGGAG TGCCCTGTAT TCCCAACAGA ACTCGCCTTT 
GCCCTGGATA CCTCTGAGGG GGTGACTCAG GACACCTTCA GCCGGATGCG AGAGGTGCTT 
CTGGGCATTG TGGGCGATCT GACCATTGCA GAGAGCAACT GTCCGCGGGG TGCCCGCGTG 
GCTGTGGTCA CCTATAACAA TGAGGTGACT ACAGAGATCC GGTTTGCTGA CTCCAAGAAG 
AAGTCTGCCC TCTTAGACAG TATTCAGAAC CTCCAAGTGG CCTTGACCTC TAAGCAGCAG 
AGCCTGGAGA CCGCAATGTC ATTCGTGGCC AGGAATACAT TCAAACGTGT GAGGAGTGGC 
TTCCTGATGA GGAAAGTGGC TGTTTTCTTC AGCAACAAGC CCACCAGGGC ATCCCCCCAG 
CTCCGAGAGG CCGTACTCAA GCTTTCCGAT GCGGGGATCA CACCCTTGTT CCTTACAAGC 
CAGGAGGACC GACAGCTCAT CAACGCTTTG CAGATCAATA ACACAGCAGT GGGACATGCC 
CTGGTTCTGC CTGCAAGGAG GGATCTCACA GACTTCCTGA AGAATGTCCT TACCTGCCAT 
GTTTGCTTGG ACATTTGCAA TATCGACCCA TCCTGTGGCT TCGGCAGCTG GAGGCCTTCC 
TTCAGGGACC GGAGGGCAGC AGGCAGTGAT GTGGACATAG ACCTGGCTTT CATCTTGGAC 
AGCTCAGAGG CTACCACACT GTTCCAGTTC AATGAGATGA AGAAATACAT AGGCTATGTG 
ATCAGACAGC TGGACCTGAG CCCAGACCCG AAGGCTTCCC AACACTTTGC CAGGGTGGCT 
GTTGTGCAGC AGTCAACCTA CGAGTCTGTG GACAATGCCA GTGTGCCGCC TGTGAAGGTG 
GAATTCTCAC TGACAGACTA TGGTGCCAAG GAAAAGCTGT TGGATTTCCT TAGCCGGAGG 
ATGACCCAAC TTCAGGGAAC CATGGGCCTG GGCAATGCCA TTGAATACAC TATAGAGAAC 
ATCTTTGAAA GTGCTCCAAA CCCACGGGAC CTCAAAATTA TGGTGCTGAT GTTGACTGGT 
GACATGCAGA GGCAGCAGCT GGAGGAGGCC CAGAGAGCCA TCCTCCAGGC CAAGTGCAAG 
GGTTACTTCT TTGTGGTTCT GGGCATTGGC AGGAAGGTGA ATATCAAGGA GGTCTACAGC 
TTTGCCAGTG AGCCCAATGA TGTCTTCTTC AAATTTGTGG ACAAATCAAC TGAGCTCAAC 
GAGGAGCCTC TGATGCGCTT TGGGAGGCTG CTCCCATCCT TTGTCAGCAG TGAAAATGCA 
TTTTACCTGC CTCCAGATCT CAGGAAACAG TGTGATTGGT TCCAAGGGGA CCAACCAGTG 
AAGAATGGCG TGAAGTTTGG TCACAAACAG ATAAACCCTC CACATACTGC TAATTCAAGT 
CTTACATCCA AAGTTGTGAC CACAATGAAG CCAGTGACAA CGACCAAACC AACAGCAATT 
GTAAACCTGC CACCTGCTAA GCCAGCCCCA GCGAGGCCAG CCCCTGCTCA GCCAGTTCTT 
GCTAAGCCAG ACCCCGCAAA ACCAGCCCAA GCAAGGCCAG CCCCTGCTAA GCCAGCTTCT 
GCTAAGTTGG TGCCTCCTCA GCCAGTCCAT GTTCAGCCAG CCCCTGCCCA GACAGCTTCT 
GTAAGACCAG CCCCCGCTAA GCCAGCCCCT CCTCAGCCAG CTGCTGCTAA GCCAGTCCCT 
GCTAAGCCAG CAGTCCCTGC TCAGCCAGCC CCTCCTCAGC CAGCTGCTGC TAAGCCAGTC 
CCTGCTAAGC CAGCAGTCCC TGCTCAGCCA GCCCCTCCTC AGCCAGCTGC TGCTAAGCCA 
GTCCCTGCTA AGCCAGCAGT CCCTGCTCAG CCAGCCGCTG CTCAACCAAT GCCTGCTCAG 
CCAGTCCTTA CTAAATCAGC CGCTGTGAAA CCAGCCTCTG CCAACAAGCC TGTGGCAGCC 
AAACCTGTGG CCACGAACAC AGCCACAGCC ACAGCCAGAC CTGCATTAGC AGCGAAGCCT 
GCTGCAGCGA AGCCTGCAGC AACGAGACCC CTTGCTGCTG CCATAAGGCC AGTTGCTACC 
AAGCCAGAGG CCCCCAGGCA ACAGGCCAAA CCAGCTGCCA CCAAGCCAGC CACTACTAAG 
CCCTTGGCGA GAGTGTCCCG AGAAGTCCAG GTCTCTGAAG TCACGGAGAA CAGTGCCAGA 
CTCCACTGGG AGCGGCCCGA GCCTTCCAGC TCCTTTTTTT ATGACCTCAC TGTAACCTCG 
GCCCACGACC AGTCCTTGGT TCTGAGACAA AACCTCACTG TCACTGACCG TGTCATCGGA 
GGCCTACTGG CTGGACAGCT GTACCATGTC GTAGTGGTCT CCTACCTGCA GTCTCAGGTC 
AGGGCCATCT ACCAAGGAAG TTTCAATACG AAGAAAACCC AGCCTCCACC TCTTCAGGCA 
GCACACCGAG CATCCAGTTC AACCATCAAC CTCATGGTGA ACACAGAACC ATTGTTTCTC 
ACTAAAACAG ATATATGTAA GCTGTCCAGA GATGCTGGGA CTTGTGTGGA CTTCAAGTTA 
CTATGGCACT ATGACCTAGA GAGCAAAAGT TGCAAGAGAT TCTGGTATGG AGGTTGTGGA 
GGCAACGAGA ACAGATTCCA CTCCCAGGAA GAATGTGAAA AGATGTGTAG TCCTGAGTTA 
ACAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a3 ( NM_001243008.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001229937.1 Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant 1, mRNA.

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CloneID	OMu03629
Clone ID Related Accession (Same CDS sequence)	NM_001243008.1
Accession Version	NM_001243008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	collagen alpha-3(VI) chain isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ098732.1, AF034136.1, AC158773.6, CN667533.1, AK076391.1, CF734596.1, BQ561449.1, BQ560536.1, AC110895.14, FQ976558.1, AA118378.1 and BX638221.1. On or before Jul 15, 2011 this sequence version replaced XM_003085326.1, XM_003086179.1, XM_003086177.1, XM_003086176.1, XM_003086178.1, XM_003086180.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAAAC ACCGGCATCT GCCCCTAGTG GCTGTCTTTA GCCTTCTCCT CTCAGGCATT 
GCCACGACTC ATGCCCAACA GCATGGAGAT GTCAAAAACG GTGCGGCGGC TGACATAGTG 
TTTCTAGTGG ATTCCTCTTG GAGTGCTGGG AAGGACCGTT TCCTGCTTGT TCAAGAGTTT 
CTGTCTGATG TTGTAGAGTC TTTAGCTGTG GGAGACAATG ATTTTCACTT TGCTCTGGTC 
CGGCTCAACG GAAACCCACA TACCGAGTTC CTGTTAAATA CATACCACTC TAAACAAGAA 
GTCCTTTCTC ATATTGTGAA CATGTCTTAC ATTGGGGGAA GCAACCAAAC TGGAAAAGGA 
TTAGAATACG TAATTCACAG CCACCTCACT GAGGCTTCCG GAAGCCGGGC TGCTGACGGA 
GTCCCTCAGG TTATCATAGT ATTAACTGAT GGACAATCGG AGGATGGCTT CGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC GGACGTTAAT GTGTTTGCAG TCGGAGTTGA GGGTGCAGAT 
GAAAGAGCCC TAGGAGAAGT AGCAAGCGAA CCACTCAGTA TGCATGTTTT CAACCTAGAG 
AACGTTACCT CACTGCATGG CCTAGTAGGG AACTTAGTGT CCTGTATACA CTCCTCTGTG 
AATCCAGAAA GGGCTGGGGA CAAGGAAAGC CTTAAAGACA TCACAGCACA AGACTCAGCT 
GACATTATTT TCCTTATTGA TGGATCACAG AACACCGGAA ATGCCAATTT CGATGTCATT 
CGAGACTTCC TTGTAAATGT CCTTGAGAGA CTCTCAGTTG GAAACCAGCA GGTCCAAGTG 
GGGGTGGTAC AGTATAGCGA GGAGCCCATA ACCATGTTCT CCTTGAATAG CTACCCCTCC 
AAGGCTGCAG TTCTGGATGC CGTGAAAGGG CTCAGCTTGG TTGGGGGGGA GTCGGCCAAT 
ATAGGCCAGG CTCTTGACTT TGTAGTGGAG AATCACTTCA CTCGGGCAGG TGGCAGCCGC 
GTGGAGGAAG GGGTCCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATTCGAGATT CGGTGGTAGC TCTGAAGCAG GCTAGTGTGT TCTCGTTTGG CCTCGGAGCC 
CAGGCAGCCT CCAGGGCAGA GCTTCAGCAC ATAGCTACCG ATGACAGCTT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCTT TGGGGATCTC CAGGAGCAAA TACTGCCGTA CCTTGTTGGT 
GTGGCCCAAA GGCACATTGT CTTGCAACCA CCAGCTATTG TCACACAAGT CATGGAAGTC 
AACAAGAGGG ACATAGTCTT CCTGGTGGAT GGTTCCTCCA GCCTGGGGCC ATCCAACTTC 
AATGCCATCC GAGACTTCGT TACCAGAGTC ATCCAGAGGT TGGAAATTGG ACAGGACCTG 
GTTCAGGTAT CAGTGGCTCA GTATGCAGAC ACTGTCAAGC CTGAGTTCTA TTTAAATTCC 
TATACCAACA AAAGGGATGC CATAACTGCT GTACGGAAGA TGAGGGCCCT GAATGGCTCA 
GCCCTGTACA CGGGATCTTC TCTGGACTTT GTTCGAAACA ACCTCTTCAC TAGCTCAGCC 
GGCCATAGGG CCGCTGAGGG GGTTCCTAAG CTCCTGGTGC TGATCACAGG CGGTAAGTCC 
CTCGATGAAG TCAGCCAGCC TGCCCAGGAG CTGAAGAGAG GCAGCATCAT GGCCTTGGCC 
GTTGGCAGCA AGGCCGCCGA TGAGGATGAG CTGAAAGAGA TCGCTTTCGA CTCCTCCCTG 
GTCTTCATTC CCGCTGAGTT CCGCCCTGCT CCCTTGCAAA ACATGCTGCC CAGCCTGATG 
GCACCTCTCA GGACTCTCAC TGGAACCACG GAAGTTCACG TAAACAAACG GGATATCATC 
TTCCTTTTGG ATGGCTCCGA CAACGTTGGG AAAAACAATT TCCCTTATGT GCGTGACTTT 
GTAACCAACC TAGTTAACAG CCTCGATGTT GGAAGTGACA ATATCCGTGT TGGCTTGGTG 
CAGTTTAGTG ACACTCCGGT CACGGAGTTC TCTCTAGACA CTTACCAGAC CAAGTCCGAG 
CTGCTCGCTC ACCTGAGGCG CTTGCAGCTC AAGGGGGGCT CCGGCTTGAA CGCAGGCTCT 
GCCTTGAGCT ATATCCATGC CAATCACTTC ACGGAGGCAG GAGGCAGCAG GACTCGTGAA 
CACGTGCCTC AGCTGCTGCT CCTGCTCATG GCCGGGCCCT CTGAGGATGC CTATTTGCAA 
GCTGCCAATG CCCTGGTGCG CTCAGGCGTG CTGACCTTCT GTGTGGGGAC CAATCGGGCG 
GATAAGGCTG AGTTAGAGCA TATTGCTTTT AATCCCAGCC TGGTATACCT CATGGACGAT 
TTCCGCTCCC TGCCATCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC ATATGTTAGT 
GGAGGTGTAC AGGAAGTTCC ACTATCCCAG CCAGAGAGCA AGCGGGACAT TCTTTTCCTC 
TTTGACGGCT CAGTCAATGT CTTGGGTCAG TTCCCTGCTG TCAGAGACTT TCTCTACAGG 
ATCATTGAGG AACTTGATGT GAAGCCGGAT GGGACCCGGG TAGCAATAGC TCAGTTCAGT 
GATGACGTCA GGCTGGAGTC CCGCTTCAGT GAGCACCAGA CGAAAGCTGA AATCCTTAAT 
CTCGTGAAGA AGATGAAGCT AAAAACAGGC AAAGCCCTCA ACCTGGGCTA CGCCCTGGAC 
TACGCGCTTA GGAACATCTT TGTGAGGTCG GCTGGCAGCC GCATCGAGGA TAACGTGCAG 
CAGTTCCTGG TGCTGCTGGT TGCTGGAAGG TCGTCAGATG CTGTGGCCGG GCCTGCAAGC 
TCCCTGAAGC AGAGAGGAGT GGTACCTTTC ATCTTTCAAG CCAAGAACGC CAACCCCAGT 
GAGCTGGAGC AGATTGTGCT GTCGCCTGCA TTCATCCTGG CTGCAGAGTC ACTCCCTAAG 
ATCGGAGACC TCCAATCACA GATTGTGAGC CTTCTGAAAG CAGAACAGGG TTCAGGCCCT 
GTATCAGGTG AAAAGGATGT GGTGTTTCTC ATTGATGGCT CTGAGGGTGT CCGCAGTGGC 
TTCCCCCTGC TAAAGGACTT TGTGCAGAGG GTTGTGGAGA GCTTGGATGT GGGTCCCGAC 
CGTGTGCGTG TGGCACTGGT GCAGTACAGT GACCGGACCA GGCCGGAGTT CTACCTGAAT 
TCCCACATGG ACCAGCAGGG TGTCATCAGC GCCATCCGCA GGCTGACACT GCTGGGCGGC 
CCAACCCCCA ACACAGGGGC GGCACTGGAG TTCGTGTTAA GGAACATTCT GACCAGTTCT 
ACTGGGAGCA GGATAGCAGA AGGTGTTCCT CAGCTCCTGA TTGTCCTCAC AGCAGAGCCA 
TCAGGGGATG ACGTGCGAGG CCCTTCAGTA GTCCTGAAGC AGGGCGGGGC TGTGCCCATT 
GGCATTGGCA TTGGGAATGC TGACATCTCT GAAATGCAGA CCATCTCCTT CATCCCTGAC 
TTTGCTGTGG CCATCCCCAC CTTCCGGGAG CTTGGGACGA TACAGCAGGT CATCTCTGAG 
AGGGTGATCC AGCTTAATCG TGAGGAGCTG AGCTCGTTGA AACCCATTTT GACGCCCTCA 
ACAGGTGCAG GTGTTGGCAG CAAGAAAGAT GTGGTCTTTC TCATCGATGG ATCCCGAAAT 
GCTGGCCCTG AGTTCCAGTA CATCCGTACC CTGATTGAGA GGATAGTCGA GTACCTGGAC 
ATAGGCTTTG ACACCACCCG GGTAGCCGTC ATCCAGTTCA GTGAGGACTC CAAGATGGAG 
TTTCCTCTGA ATGCCCACTT CAGCAAGGAT GAAGTACAGA ACGCTGTGCG CAGGCTGCGG 
CCCAAGGGTG GGAGTCAGGT CTACATCGGA AATGCCCTGG AGTACGTGCT GAAGAACATC 
TTCCAGAGGC CACTGGGGAG CCGGATAGAA GAGGGCGTTC CGCAGTTCCT AGTCCTCATT 
TCGTCTGGAA AGTCTGATGA TGAGGTGGAC GACTCAGCCG TGGAACTCAA GCAGTTCGGT 
GTGGCCCCTC TCACCATAGC CAGACACACA GACCAAGAGG AGCTGGTGAA GATCTCCCTA 
AGTCCTGAGT ACGTGTACTC CGTGAGCACC TTCAGGGAGC TGCCCAGACT GGAGCAGAAG 
CTGCTGACGC CCATCACCAC TCTAACCTCA CAGCAGATCC ATCAGATCCT GGCCAGTACC 
CGATATCCCC CTTCAGTGGT TGAAAGCGAT GCGGCTGACA TCGTCTTTCT GATTGACAGC 
TCTGATGCCG TCAAGCCCGA TGGCATCGCT CATATCCGAG ACTTTGTCAG CAGGATCGTT 
CGCAGACTCA ACATTGGTCC CAGTAAAGTG AGGATTGGGG TCGTGCAGTT TAGCAATGAT 
GTCTTCCCTG AGTTCTACCT GAAGACCCAC AAGTCCCAGA GCAGTGTGCT TGAAGCCATA 
CGGCGTCTGA GGTTCAAAGG AGGGTCTCCC CTAAACACTG GCAGAGCCCT GGAGTTTGTG 
GCAAGAAACC TCTTCGTGAA GTCTGCTGGG AGCCGGATAG AAGATGGGGT CCCTCAACAC 
CTGGTCTTAT TCCTGGGTGG GAAGTCTCAG GACGATGTGG CCAGGCATGC CCAAGTCATA 
AGCTCATCGG GGATTGTGAG CTTAGGAATA GGAGATCGGA ACATTGATAG GACGGACTTG 
CAAACCATCA CCAATGACCC CAGGCTGGTC TTCACAGTTC GGGAATTCAG AGAGCTGCCC 
AACATAGAAG AGAGGGTCAT GCTTTCCTTT GGACCCTCTG GGGCTACTCC TCAGCCTCCT 
GGGGTAGATT TACCTTCTCC TTCCCGGCCA GAGAAGAAGA AGGCCGACAT TGTGTTCCTG 
CTGGATGGTT CCATCAATTT TAGGAGGGAC AGTTTCCAGG AAGTGCTCCG TTTTGCATCT 
GAAATCGTGG ACACGGTCTA TGAAGACGGA GATTCCATCA GAGTGGGACT GGTCCAGTAC 
AACTCAGACC CCACGGATGA ATTCTTCCTG AGGGACTTTT CTACCAAAAG GCAGATTATC 
GATGCCATCA ACAAAGTCGT CTATAAAGGA GGGAGGCATG CCAACACCAG GGTGGGCATC 
GAGCACCTGC TGCGGAATCA CTTTGTGCCG GAGGCTGGTA GCCGCCTGGA TGAGCGGGTT 
CCTCAGATCG CCTTCGTGAT CACCGGAGGG AAGTCAGTGG AGGATGCTCA GGATGTGAGC 
CTGGCTCTCA CCCAGAAAGG TGTCAAGGTG TTTGCCGTTG GCGTGAGGAA CATCGACTCT 
GAGGAGGTGG GCAAGATAGC CTCCAACAGT GCCACAGCCT TCCGGGTGGG CAGCGTGCAG 
GAACTGTCCG AACTGAGTGA GACAGTGCTC GAGACTCTGC ATGATGCAAT GCATGAGACC 
CTCTGCCCTG GTGTGACCGA TGTCTCCAAA GCCTGCAATC TGGAAGTCAT TCTGGGGTTT 
GATGGATCTC GAGATCAGAA TGTGTTTGTG AGTCAGAAGG GCCTTGAGTC CAAGGTGGAC 
ATCATCTTGA ACAGAATCAG CCAGATACAA AGGATCAGCT GCAGTGGCAA CCAGCTGCCC 
ACTGTGAGGG TATCCGTGAT GGCGAACACG CCCTCTGGGC CAGTGGAGGC CTTTGACTTT 
GCCGAGTATC AGCCGGAGCT GTTTGAGAAG TTTCGCAACA TGCGCAGTCA GCGCCCATAC 
GTCCTCACAG CTGACACGCT GAAGTTGTAC CAGAACAAGT TCAGGCAGTC GTCGCCTGAT 
ACTGTGAAGG TAGTCATTCA CTTCACGGAT GGAGCAGATG GAGACATGGC TGACTTATAC 
AGAGCATCTG AGGAACTCCG GCAAGCAGGT GCCCAAGCCC TGATCCTGGT GGGTCTTGAA 
CGTGTGGCTA ACCTGGAGCG GCTGATGCAT CTGGAGTTCG GGCGGGGCTT CATGTATGAC 
AGGCCCCTGA GGCTCAACCT GCTTGACTTA GACTATGAAC TGGCAGAGCA GCTTGACAAT 
ATTGCTGAGA AAGCTTGCTG TGGGGTTCCA TGCAAGTGCT CTGGAGAGAG GGGAGACAGA 
GGGCCCATTG GCAGCATTGG GCCAAAGGGT ATCTCCGGGG AAGATGGCTA CCGAGGCTAC 
CCGGGAGACG AAGGCGGACC TGGTGAGCGA GGTCCACCTG GTGTGAATGG CACACAAGGT 
TTCCAGGGCT GTCCAGGCCA GAGGGGAGTG AAGGGTTCTC GAGGATTCCC AGGAGAGAAG 
GGTGAATTGG GAGAAATCGG CTTGGATGGT CTGGATGGTG AAGAGGGAGA CAAGGGGCTG 
CCTGGTTCTT CTGGAGAAAA GGGGAGTCCT GGAAGAAGGG GTGACAAAGG ACCCAAAGGA 
GACAAAGGCG AGAGAGGAGA TGTCGGGATT CGAGGTGACC CGGGTGACTC AGGACGGGAC 
AGCCAGCAGA GAGGACCCAA AGGAGAGACG GGAGACATCG GCCCCATGGG TCTCCCAGGC 
AGAGATGGCA TCCCTGGCAG CCCAGGAGAC CCTGGGAAGG ACGGTGGCTC TGGCCGAAGG 
GGCCCTGCAG GAGCTAAGGG CAACAGAGGT GGTCCTGGCC AGCCAGGCTT CGAAGGCGAG 
CAGGGTACCA GAGGCTCACA GGGCCCACCT GGTCCCATTG GTCCCCCAGG CCTGATTGGG 
GAACAAGGAA TTCCCGGACC TCGAGGAGGC GGAGGTACGG CTGGGGCTCC TGGAGAACGT 
GGCAGAACTG GTCCTCTGGG GAGGAAGGGT GAGCCAGGAG AGCCAGGGCC GAAGGGAAGC 
ATCGGGAACC GTGGACCCCG AGGAGAGACG GGAGATGACG GGAGAGATGG GGTTGGCAGT 
GAAGGACGCA GAGGCAAAAA AGGAGAAAGA GGCTTCCCTG GGTATCCGGG ACCAAAGGGC 
ACCCCTGGTG AGCCAGGGGC AGATGGACCA CCAGGACCCA AAGGCATCAG AGGTCGAAGG 
GGAAATTCAG GACCTCCAGG GGCAACTGGA CAAAAGGGAG ACCCTGGTTA CCCAGGACCA 
TCTGGTCACA AGGGCAACCG AGGCGACTCC GTTGATCAAT GCGCCCTCAT CCAGAGCATC 
AGAGACAAAT GCCCTTGCTG CTACGGGCCC CTGGAGTGCC CTGTATTCCC AACAGAACTC 
GCCTTTGCCC TGGATACCTC TGAGGGGGTG ACTCAGGACA CCTTCAGCCG GATGCGAGAG 
GTGCTTCTGG GCATTGTGGG CGATCTGACC ATTGCAGAGA GCAACTGTCC GCGGGGTGCC 
CGCGTGGCTG TGGTCACCTA TAACAATGAG GTGACTACAG AGATCCGGTT TGCTGACTCC 
AAGAAGAAGT CTGCCCTCTT AGACAGTATT CAGAACCTCC AAGTGGCCTT GACCTCTAAG 
CAGCAGAGCC TGGAGACCGC AATGTCATTC GTGGCCAGGA ATACATTCAA ACGTGTGAGG 
AGTGGCTTCC TGATGAGGAA AGTGGCTGTT TTCTTCAGCA ACAAGCCCAC CAGGGCATCC 
CCCCAGCTCC GAGAGGCCGT ACTCAAGCTT TCCGATGCGG GGATCACACC CTTGTTCCTT 
ACAAGCCAGG AGGACCGACA GCTCATCAAC GCTTTGCAGA TCAATAACAC AGCAGTGGGA 
CATGCCCTGG TTCTGCCTGC AAGGAGGGAT CTCACAGACT TCCTGAAGAA TGTCCTTACC 
TGCCATGTTT GCTTGGACAT TTGCAATATC GACCCATCCT GTGGCTTCGG CAGCTGGAGG 
CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATAGACCT GGCTTTCATC 
TTGGACAGCT CAGAGGCTAC CACACTGTTC CAGTTCAATG AGATGAAGAA ATACATAGGC 
TATGTGATCA GACAGCTGGA CCTGAGCCCA GACCCGAAGG CTTCCCAACA CTTTGCCAGG 
GTGGCTGTTG TGCAGCAGTC AACCTACGAG TCTGTGGACA ATGCCAGTGT GCCGCCTGTG 
AAGGTGGAAT TCTCACTGAC AGACTATGGT GCCAAGGAAA AGCTGTTGGA TTTCCTTAGC 
CGGAGGATGA CCCAACTTCA GGGAACCATG GGCCTGGGCA ATGCCATTGA ATACACTATA 
GAGAACATCT TTGAAAGTGC TCCAAACCCA CGGGACCTCA AAATTATGGT GCTGATGTTG 
ACTGGTGACA TGCAGAGGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT CCAGGCCAAG 
TGCAAGGGTT ACTTCTTTGT GGTTCTGGGC ATTGGCAGGA AGGTGAATAT CAAGGAGGTC 
TACAGCTTTG CCAGTGAGCC CAATGATGTC TTCTTCAAAT TTGTGGACAA ATCAACTGAG 
CTCAACGAGG AGCCTCTGAT GCGCTTTGGG AGGCTGCTCC CATCCTTTGT CAGCAGTGAA 
AATGCATTTT ACCTGCCTCC AGATCTCAGG AAACAGTGTG ATTGGTTCCA AGGGGACCAA 
CCAGTGAAGA ATGGCGTGAA GTTTGGTCAC AAACAGATAA ACCCTCCACA TACTGCTAAT 
TCAAGTCTTA CATCCAAAGT TGTGACCACA ATGAAGCCAG TGACAACGAC CAAACCAACA 
GCAATTGTAA ACCTGCCACC TGCTAAGCCA GCCCCAGCGA GGCCAGCCCC TGCTCAGCCA 
GTTCTTGCTA AGCCAGACCC CGCAAAACCA GCCCAAGCAA GGCCAGCCCC TGCTAAGCCA 
GCTTCTGCTA AGTTGGTGCC TCCTCAGCCA GTCCATGTTC AGCCAGCCCC TGCCCAGACA 
GCTTCTGTAA GACCAGCCCC CGCTAAGCCA GCCCCTCCTC AGCCAGCTGC TGCTAAGCCA 
GTCCCTGCTA AGCCAGCAGT CCCTGCTCAG CCAGCCCCTC CTCAGCCAGC TGCTGCTAAG 
CCAGTCCCTG CTAAGCCAGC AGTCCCTGCT CAGCCAGCCC CTCCTCAGCC AGCTGCTGCT 
AAGCCAGTCC CTGCTAAGCC AGCAGTCCCT GCTCAGCCAG CCGCTGCTCA ACCAATGCCT 
GCTCAGCCAG TCCTTACTAA ATCAGCCGCT GTGAAACCAG CCTCTGCCAA CAAGCCTGTG 
GCAGCCAAAC CTGTGGCCAC GAACACAGCC ACAGCCACAG CCAGACCTGC ATTAGCAGCG 
AAGCCTGCTG CAGCGAAGCC TGCAGCAACG AGACCCCTTG CTGCTGCCAT AAGGCCAGTT 
GCTACCAAGC CAGAGGCCCC CAGGCAACAG GCCAAACCAG CTGCCACCAA GCCAGCCACT 
ACTAAGCCCT TGGCGAGAGT GTCCCGAGAA GTCCAGGTCT CTGAAGTCAC GGAGAACAGT 
GCCAGACTCC ACTGGGAGCG GCCCGAGCCT TCCAGCTCCT TTTTTTATGA CCTCACTGTA 
ACCTCGGCCC ACGACCAGTC CTTGGTTCTG AGACAAAACC TCACTGTCAC TGACCGTGTC 
ATCGGAGGCC TACTGGCTGG ACAGCTGTAC CATGTCGTAG TGGTCTCCTA CCTGCAGTCT 
CAGGTCAGGG CCATCTACCA AGGAAGTTTC AATACGAAGA AAACCCAGCC TCCACCTCTT 
CAGGCAGCAC ACCGAGCATC CAGTTCAACC ATCAACCTCA TGGTGAACAC AGAACCATTG 
TTTCTCACTA AAACAGATAT ATGTAAGCTG TCCAGAGATG CTGGGACTTG TGTGGACTTC 
AAGTTACTAT GGCACTATGA CCTAGAGAGC AAAAGTTGCA AGAGATTCTG GTATGGAGGT 
TGTGGAGGCA ACGAGAACAG ATTCCACTCC CAGGAAGAAT GTGAAAAGAT GTGTAGTCCT 
GAGTTAACAG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229937.1
CDS	255..10109
Translation	MRKHRHLPLVAVFSLLLSGIATTHAQQHGDVKNGAAADIVFLVDSSWSAGKDRFLLVQEFLSDVVESLAVGDNDFHFALVRLNGNPHTEFLLNTYHSKQEVLSHIVNMSYIGGSNQTGKGLEYVIHSHLTEASGSRAADGVPQVIIVLTDGQSEDGFALPSAELKSADVNVFAVGVEGADERALGEVASEPLSMHVFNLENVTSLHGLVGNLVSCIHSSVNPERAGDKESLKDITAQDSADIIFLIDGSQNTGNANFDVIRDFLVNVLERLSVGNQQVQVGVVQYSEEPITMFSLNSYPSKAAVLDAVKGLSLVGGESANIGQALDFVVENHFTRAGGSRVEEGVPQVLVLISAGPSSDEIRDSVVALKQASVFSFGLGAQAASRAELQHIATDDSLVFTVPEFRSFGDLQEQILPYLVGVAQRHIVLQPPAIVTQVMEVNKRDIVFLVDGSSSLGPSNFNAIRDFVTRVIQRLEIGQDLVQVSVAQYADTVKPEFYLNSYTNKRDAITAVRKMRALNGSALYTGSSLDFVRNNLFTSSAGHRAAEGVPKLLVLITGGKSLDEVSQPAQELKRGSIMALAVGSKAADEDELKEIAFDSSLVFIPAEFRPAPLQNMLPSLMAPLRTLTGTTEVHVNKRDIIFLLDGSDNVGKNNFPYVRDFVTNLVNSLDVGSDNIRVGLVQFSDTPVTEFSLDTYQTKSELLAHLRRLQLKGGSGLNAGSALSYIHANHFTEAGGSRTREHVPQLLLLLMAGPSEDAYLQAANALVRSGVLTFCVGTNRADKAELEHIAFNPSLVYLMDDFRSLPSLPQQLIQPLTTYVSGGVQEVPLSQPESKRDILFLFDGSVNVLGQFPAVRDFLYRIIEELDVKPDGTRVAIAQFSDDVRLESRFSEHQTKAEILNLVKKMKLKTGKALNLGYALDYALRNIFVRSAGSRIEDNVQQFLVLLVAGRSSDAVAGPASSLKQRGVVPFIFQAKNANPSELEQIVLSPAFILAAESLPKIGDLQSQIVSLLKAEQGSGPVSGEKDVVFLIDGSEGVRSGFPLLKDFVQRVVESLDVGPDRVRVALVQYSDRTRPEFYLNSHMDQQGVISAIRRLTLLGGPTPNTGAALEFVLRNILTSSTGSRIAEGVPQLLIVLTAEPSGDDVRGPSVVLKQGGAVPIGIGIGNADISEMQTISFIPDFAVAIPTFRELGTIQQVISERVIQLNREELSSLKPILTPSTGAGVGSKKDVVFLIDGSRNAGPEFQYIRTLIERIVEYLDIGFDTTRVAVIQFSEDSKMEFPLNAHFSKDEVQNAVRRLRPKGGSQVYIGNALEYVLKNIFQRPLGSRIEEGVPQFLVLISSGKSDDEVDDSAVELKQFGVAPLTIARHTDQEELVKISLSPEYVYSVSTFRELPRLEQKLLTPITTLTSQQIHQILASTRYPPSVVESDAADIVFLIDSSDAVKPDGIAHIRDFVSRIVRRLNIGPSKVRIGVVQFSNDVFPEFYLKTHKSQSSVLEAIRRLRFKGGSPLNTGRALEFVARNLFVKSAGSRIEDGVPQHLVLFLGGKSQDDVARHAQVISSSGIVSLGIGDRNIDRTDLQTITNDPRLVFTVREFRELPNIEERVMLSFGPSGATPQPPGVDLPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFASEIVDTVYEDGDSIRVGLVQYNSDPTDEFFLRDFSTKRQIIDAINKVVYKGGRHANTRVGIEHLLRNHFVPEAGSRLDERVPQIAFVITGGKSVEDAQDVSLALTQKGVKVFAVGVRNIDSEEVGKIASNSATAFRVGSVQELSELSETVLETLHDAMHETLCPGVTDVSKACNLEVILGFDGSRDQNVFVSQKGLESKVDIILNRISQIQRISCSGNQLPTVRVSVMANTPSGPVEAFDFAEYQPELFEKFRNMRSQRPYVLTADTLKLYQNKFRQSSPDTVKVVIHFTDGADGDMADLYRASEELRQAGAQALILVGLERVANLERLMHLEFGRGFMYDRPLRLNLLDLDYELAEQLDNIAEKACCGVPCKCSGERGDRGPIGSIGPKGISGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEEGDKGLPGSSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGDSGRDSQQRGPKGETGDIGPMGLPGRDGIPGSPGDPGKDGGSGRRGPAGAKGNRGGPGQPGFEGEQGTRGSQGPPGPIGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGEPGPKGSIGNRGPRGETGDDGRDGVGSEGRRGKKGERGFPGYPGPKGTPGEPGADGPPGPKGIRGRRGNSGPPGATGQKGDPGYPGPSGHKGNRGDSVDQCALIQSIRDKCPCCYGPLECPVFPTELAFALDTSEGVTQDTFSRMREVLLGIVGDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSALLDSIQNLQVALTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRASPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPARRDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDLAFILDSSEATTLFQFNEMKKYIGYVIRQLDLSPDPKASQHFARVAVVQQSTYESVDNASVPPVKVEFSLTDYGAKEKLLDFLSRRMTQLQGTMGLGNAIEYTIENIFESAPNPRDLKIMVLMLTGDMQRQQLEEAQRAILQAKCKGYFFVVLGIGRKVNIKEVYSFASEPNDVFFKFVDKSTELNEEPLMRFGRLLPSFVSSENAFYLPPDLRKQCDWFQGDQPVKNGVKFGHKQINPPHTANSSLTSKVVTTMKPVTTTKPTAIVNLPPAKPAPARPAPAQPVLAKPDPAKPAQARPAPAKPASAKLVPPQPVHVQPAPAQTASVRPAPAKPAPPQPAAAKPVPAKPAVPAQPAPPQPAAAKPVPAKPAVPAQPAPPQPAAAKPVPAKPAVPAQPAAAQPMPAQPVLTKSAAVKPASANKPVAAKPVATNTATATARPALAAKPAAAKPAATRPLAAAIRPVATKPEAPRQQAKPAATKPATTKPLARVSREVQVSEVTENSARLHWERPEPSSSFFYDLTVTSAHDQSLVLRQNLTVTDRVIGGLLAGQLYHVVVVSYLQSQVRAIYQGSFNTKKTQPPPLQAAHRASSSTINLMVNTEPLFLTKTDICKLSRDAGTCVDFKLLWHYDLESKSCKRFWYGGCGGNENRFHSQEECEKMCSPELTV

Target ORF information:

RefSeq Version	NM_001243008.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243008.1

ORF Insert Sequence:

ATGAGGAAAC ACCGGCATCT GCCCCTAGTG GCTGTCTTTA GCCTTCTCCT CTCAGGCATT 
GCCACGACTC ATGCCCAACA GCATGGAGAT GTCAAAAACG GTGCGGCGGC TGACATAGTG 
TTTCTAGTGG ATTCCTCTTG GAGTGCTGGG AAGGACCGTT TCCTGCTTGT TCAAGAGTTT 
CTGTCTGATG TTGTAGAGTC TTTAGCTGTG GGAGACAATG ATTTTCACTT TGCTCTGGTC 
CGGCTCAACG GAAACCCACA TACCGAGTTC CTGTTAAATA CATACCACTC TAAACAAGAA 
GTCCTTTCTC ATATTGTGAA CATGTCTTAC ATTGGGGGAA GCAACCAAAC TGGAAAAGGA 
TTAGAATACG TAATTCACAG CCACCTCACT GAGGCTTCCG GAAGCCGGGC TGCTGACGGA 
GTCCCTCAGG TTATCATAGT ATTAACTGAT GGACAATCGG AGGATGGCTT CGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC GGACGTTAAT GTGTTTGCAG TCGGAGTTGA GGGTGCAGAT 
GAAAGAGCCC TAGGAGAAGT AGCAAGCGAA CCACTCAGTA TGCATGTTTT CAACCTAGAG 
AACGTTACCT CACTGCATGG CCTAGTAGGG AACTTAGTGT CCTGTATACA CTCCTCTGTG 
AATCCAGAAA GGGCTGGGGA CAAGGAAAGC CTTAAAGACA TCACAGCACA AGACTCAGCT 
GACATTATTT TCCTTATTGA TGGATCACAG AACACCGGAA ATGCCAATTT CGATGTCATT 
CGAGACTTCC TTGTAAATGT CCTTGAGAGA CTCTCAGTTG GAAACCAGCA GGTCCAAGTG 
GGGGTGGTAC AGTATAGCGA GGAGCCCATA ACCATGTTCT CCTTGAATAG CTACCCCTCC 
AAGGCTGCAG TTCTGGATGC CGTGAAAGGG CTCAGCTTGG TTGGGGGGGA GTCGGCCAAT 
ATAGGCCAGG CTCTTGACTT TGTAGTGGAG AATCACTTCA CTCGGGCAGG TGGCAGCCGC 
GTGGAGGAAG GGGTCCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATTCGAGATT CGGTGGTAGC TCTGAAGCAG GCTAGTGTGT TCTCGTTTGG CCTCGGAGCC 
CAGGCAGCCT CCAGGGCAGA GCTTCAGCAC ATAGCTACCG ATGACAGCTT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCTT TGGGGATCTC CAGGAGCAAA TACTGCCGTA CCTTGTTGGT 
GTGGCCCAAA GGCACATTGT CTTGCAACCA CCAGCTATTG TCACACAAGT CATGGAAGTC 
AACAAGAGGG ACATAGTCTT CCTGGTGGAT GGTTCCTCCA GCCTGGGGCC ATCCAACTTC 
AATGCCATCC GAGACTTCGT TACCAGAGTC ATCCAGAGGT TGGAAATTGG ACAGGACCTG 
GTTCAGGTAT CAGTGGCTCA GTATGCAGAC ACTGTCAAGC CTGAGTTCTA TTTAAATTCC 
TATACCAACA AAAGGGATGC CATAACTGCT GTACGGAAGA TGAGGGCCCT GAATGGCTCA 
GCCCTGTACA CGGGATCTTC TCTGGACTTT GTTCGAAACA ACCTCTTCAC TAGCTCAGCC 
GGCCATAGGG CCGCTGAGGG GGTTCCTAAG CTCCTGGTGC TGATCACAGG CGGTAAGTCC 
CTCGATGAAG TCAGCCAGCC TGCCCAGGAG CTGAAGAGAG GCAGCATCAT GGCCTTGGCC 
GTTGGCAGCA AGGCCGCCGA TGAGGATGAG CTGAAAGAGA TCGCTTTCGA CTCCTCCCTG 
GTCTTCATTC CCGCTGAGTT CCGCCCTGCT CCCTTGCAAA ACATGCTGCC CAGCCTGATG 
GCACCTCTCA GGACTCTCAC TGGAACCACG GAAGTTCACG TAAACAAACG GGATATCATC 
TTCCTTTTGG ATGGCTCCGA CAACGTTGGG AAAAACAATT TCCCTTATGT GCGTGACTTT 
GTAACCAACC TAGTTAACAG CCTCGATGTT GGAAGTGACA ATATCCGTGT TGGCTTGGTG 
CAGTTTAGTG ACACTCCGGT CACGGAGTTC TCTCTAGACA CTTACCAGAC CAAGTCCGAG 
CTGCTCGCTC ACCTGAGGCG CTTGCAGCTC AAGGGGGGCT CCGGCTTGAA CGCAGGCTCT 
GCCTTGAGCT ATATCCATGC CAATCACTTC ACGGAGGCAG GAGGCAGCAG GACTCGTGAA 
CACGTGCCTC AGCTGCTGCT CCTGCTCATG GCCGGGCCCT CTGAGGATGC CTATTTGCAA 
GCTGCCAATG CCCTGGTGCG CTCAGGCGTG CTGACCTTCT GTGTGGGGAC CAATCGGGCG 
GATAAGGCTG AGTTAGAGCA TATTGCTTTT AATCCCAGCC TGGTATACCT CATGGACGAT 
TTCCGCTCCC TGCCATCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC ATATGTTAGT 
GGAGGTGTAC AGGAAGTTCC ACTATCCCAG CCAGAGAGCA AGCGGGACAT TCTTTTCCTC 
TTTGACGGCT CAGTCAATGT CTTGGGTCAG TTCCCTGCTG TCAGAGACTT TCTCTACAGG 
ATCATTGAGG AACTTGATGT GAAGCCGGAT GGGACCCGGG TAGCAATAGC TCAGTTCAGT 
GATGACGTCA GGCTGGAGTC CCGCTTCAGT GAGCACCAGA CGAAAGCTGA AATCCTTAAT 
CTCGTGAAGA AGATGAAGCT AAAAACAGGC AAAGCCCTCA ACCTGGGCTA CGCCCTGGAC 
TACGCGCTTA GGAACATCTT TGTGAGGTCG GCTGGCAGCC GCATCGAGGA TAACGTGCAG 
CAGTTCCTGG TGCTGCTGGT TGCTGGAAGG TCGTCAGATG CTGTGGCCGG GCCTGCAAGC 
TCCCTGAAGC AGAGAGGAGT GGTACCTTTC ATCTTTCAAG CCAAGAACGC CAACCCCAGT 
GAGCTGGAGC AGATTGTGCT GTCGCCTGCA TTCATCCTGG CTGCAGAGTC ACTCCCTAAG 
ATCGGAGACC TCCAATCACA GATTGTGAGC CTTCTGAAAG CAGAACAGGG TTCAGGCCCT 
GTATCAGGTG AAAAGGATGT GGTGTTTCTC ATTGATGGCT CTGAGGGTGT CCGCAGTGGC 
TTCCCCCTGC TAAAGGACTT TGTGCAGAGG GTTGTGGAGA GCTTGGATGT GGGTCCCGAC 
CGTGTGCGTG TGGCACTGGT GCAGTACAGT GACCGGACCA GGCCGGAGTT CTACCTGAAT 
TCCCACATGG ACCAGCAGGG TGTCATCAGC GCCATCCGCA GGCTGACACT GCTGGGCGGC 
CCAACCCCCA ACACAGGGGC GGCACTGGAG TTCGTGTTAA GGAACATTCT GACCAGTTCT 
ACTGGGAGCA GGATAGCAGA AGGTGTTCCT CAGCTCCTGA TTGTCCTCAC AGCAGAGCCA 
TCAGGGGATG ACGTGCGAGG CCCTTCAGTA GTCCTGAAGC AGGGCGGGGC TGTGCCCATT 
GGCATTGGCA TTGGGAATGC TGACATCTCT GAAATGCAGA CCATCTCCTT CATCCCTGAC 
TTTGCTGTGG CCATCCCCAC CTTCCGGGAG CTTGGGACGA TACAGCAGGT CATCTCTGAG 
AGGGTGATCC AGCTTAATCG TGAGGAGCTG AGCTCGTTGA AACCCATTTT GACGCCCTCA 
ACAGGTGCAG GTGTTGGCAG CAAGAAAGAT GTGGTCTTTC TCATCGATGG ATCCCGAAAT 
GCTGGCCCTG AGTTCCAGTA CATCCGTACC CTGATTGAGA GGATAGTCGA GTACCTGGAC 
ATAGGCTTTG ACACCACCCG GGTAGCCGTC ATCCAGTTCA GTGAGGACTC CAAGATGGAG 
TTTCCTCTGA ATGCCCACTT CAGCAAGGAT GAAGTACAGA ACGCTGTGCG CAGGCTGCGG 
CCCAAGGGTG GGAGTCAGGT CTACATCGGA AATGCCCTGG AGTACGTGCT GAAGAACATC 
TTCCAGAGGC CACTGGGGAG CCGGATAGAA GAGGGCGTTC CGCAGTTCCT AGTCCTCATT 
TCGTCTGGAA AGTCTGATGA TGAGGTGGAC GACTCAGCCG TGGAACTCAA GCAGTTCGGT 
GTGGCCCCTC TCACCATAGC CAGACACACA GACCAAGAGG AGCTGGTGAA GATCTCCCTA 
AGTCCTGAGT ACGTGTACTC CGTGAGCACC TTCAGGGAGC TGCCCAGACT GGAGCAGAAG 
CTGCTGACGC CCATCACCAC TCTAACCTCA CAGCAGATCC ATCAGATCCT GGCCAGTACC 
CGATATCCCC CTTCAGTGGT TGAAAGCGAT GCGGCTGACA TCGTCTTTCT GATTGACAGC 
TCTGATGCCG TCAAGCCCGA TGGCATCGCT CATATCCGAG ACTTTGTCAG CAGGATCGTT 
CGCAGACTCA ACATTGGTCC CAGTAAAGTG AGGATTGGGG TCGTGCAGTT TAGCAATGAT 
GTCTTCCCTG AGTTCTACCT GAAGACCCAC AAGTCCCAGA GCAGTGTGCT TGAAGCCATA 
CGGCGTCTGA GGTTCAAAGG AGGGTCTCCC CTAAACACTG GCAGAGCCCT GGAGTTTGTG 
GCAAGAAACC TCTTCGTGAA GTCTGCTGGG AGCCGGATAG AAGATGGGGT CCCTCAACAC 
CTGGTCTTAT TCCTGGGTGG GAAGTCTCAG GACGATGTGG CCAGGCATGC CCAAGTCATA 
AGCTCATCGG GGATTGTGAG CTTAGGAATA GGAGATCGGA ACATTGATAG GACGGACTTG 
CAAACCATCA CCAATGACCC CAGGCTGGTC TTCACAGTTC GGGAATTCAG AGAGCTGCCC 
AACATAGAAG AGAGGGTCAT GCTTTCCTTT GGACCCTCTG GGGCTACTCC TCAGCCTCCT 
GGGGTAGATT TACCTTCTCC TTCCCGGCCA GAGAAGAAGA AGGCCGACAT TGTGTTCCTG 
CTGGATGGTT CCATCAATTT TAGGAGGGAC AGTTTCCAGG AAGTGCTCCG TTTTGCATCT 
GAAATCGTGG ACACGGTCTA TGAAGACGGA GATTCCATCA GAGTGGGACT GGTCCAGTAC 
AACTCAGACC CCACGGATGA ATTCTTCCTG AGGGACTTTT CTACCAAAAG GCAGATTATC 
GATGCCATCA ACAAAGTCGT CTATAAAGGA GGGAGGCATG CCAACACCAG GGTGGGCATC 
GAGCACCTGC TGCGGAATCA CTTTGTGCCG GAGGCTGGTA GCCGCCTGGA TGAGCGGGTT 
CCTCAGATCG CCTTCGTGAT CACCGGAGGG AAGTCAGTGG AGGATGCTCA GGATGTGAGC 
CTGGCTCTCA CCCAGAAAGG TGTCAAGGTG TTTGCCGTTG GCGTGAGGAA CATCGACTCT 
GAGGAGGTGG GCAAGATAGC CTCCAACAGT GCCACAGCCT TCCGGGTGGG CAGCGTGCAG 
GAACTGTCCG AACTGAGTGA GACAGTGCTC GAGACTCTGC ATGATGCAAT GCATGAGACC 
CTCTGCCCTG GTGTGACCGA TGTCTCCAAA GCCTGCAATC TGGAAGTCAT TCTGGGGTTT 
GATGGATCTC GAGATCAGAA TGTGTTTGTG AGTCAGAAGG GCCTTGAGTC CAAGGTGGAC 
ATCATCTTGA ACAGAATCAG CCAGATACAA AGGATCAGCT GCAGTGGCAA CCAGCTGCCC 
ACTGTGAGGG TATCCGTGAT GGCGAACACG CCCTCTGGGC CAGTGGAGGC CTTTGACTTT 
GCCGAGTATC AGCCGGAGCT GTTTGAGAAG TTTCGCAACA TGCGCAGTCA GCGCCCATAC 
GTCCTCACAG CTGACACGCT GAAGTTGTAC CAGAACAAGT TCAGGCAGTC GTCGCCTGAT 
ACTGTGAAGG TAGTCATTCA CTTCACGGAT GGAGCAGATG GAGACATGGC TGACTTATAC 
AGAGCATCTG AGGAACTCCG GCAAGCAGGT GCCCAAGCCC TGATCCTGGT GGGTCTTGAA 
CGTGTGGCTA ACCTGGAGCG GCTGATGCAT CTGGAGTTCG GGCGGGGCTT CATGTATGAC 
AGGCCCCTGA GGCTCAACCT GCTTGACTTA GACTATGAAC TGGCAGAGCA GCTTGACAAT 
ATTGCTGAGA AAGCTTGCTG TGGGGTTCCA TGCAAGTGCT CTGGAGAGAG GGGAGACAGA 
GGGCCCATTG GCAGCATTGG GCCAAAGGGT ATCTCCGGGG AAGATGGCTA CCGAGGCTAC 
CCGGGAGACG AAGGCGGACC TGGTGAGCGA GGTCCACCTG GTGTGAATGG CACACAAGGT 
TTCCAGGGCT GTCCAGGCCA GAGGGGAGTG AAGGGTTCTC GAGGATTCCC AGGAGAGAAG 
GGTGAATTGG GAGAAATCGG CTTGGATGGT CTGGATGGTG AAGAGGGAGA CAAGGGGCTG 
CCTGGTTCTT CTGGAGAAAA GGGGAGTCCT GGAAGAAGGG GTGACAAAGG ACCCAAAGGA 
GACAAAGGCG AGAGAGGAGA TGTCGGGATT CGAGGTGACC CGGGTGACTC AGGACGGGAC 
AGCCAGCAGA GAGGACCCAA AGGAGAGACG GGAGACATCG GCCCCATGGG TCTCCCAGGC 
AGAGATGGCA TCCCTGGCAG CCCAGGAGAC CCTGGGAAGG ACGGTGGCTC TGGCCGAAGG 
GGCCCTGCAG GAGCTAAGGG CAACAGAGGT GGTCCTGGCC AGCCAGGCTT CGAAGGCGAG 
CAGGGTACCA GAGGCTCACA GGGCCCACCT GGTCCCATTG GTCCCCCAGG CCTGATTGGG 
GAACAAGGAA TTCCCGGACC TCGAGGAGGC GGAGGTACGG CTGGGGCTCC TGGAGAACGT 
GGCAGAACTG GTCCTCTGGG GAGGAAGGGT GAGCCAGGAG AGCCAGGGCC GAAGGGAAGC 
ATCGGGAACC GTGGACCCCG AGGAGAGACG GGAGATGACG GGAGAGATGG GGTTGGCAGT 
GAAGGACGCA GAGGCAAAAA AGGAGAAAGA GGCTTCCCTG GGTATCCGGG ACCAAAGGGC 
ACCCCTGGTG AGCCAGGGGC AGATGGACCA CCAGGACCCA AAGGCATCAG AGGTCGAAGG 
GGAAATTCAG GACCTCCAGG GGCAACTGGA CAAAAGGGAG ACCCTGGTTA CCCAGGACCA 
TCTGGTCACA AGGGCAACCG AGGCGACTCC GTTGATCAAT GCGCCCTCAT CCAGAGCATC 
AGAGACAAAT GCCCTTGCTG CTACGGGCCC CTGGAGTGCC CTGTATTCCC AACAGAACTC 
GCCTTTGCCC TGGATACCTC TGAGGGGGTG ACTCAGGACA CCTTCAGCCG GATGCGAGAG 
GTGCTTCTGG GCATTGTGGG CGATCTGACC ATTGCAGAGA GCAACTGTCC GCGGGGTGCC 
CGCGTGGCTG TGGTCACCTA TAACAATGAG GTGACTACAG AGATCCGGTT TGCTGACTCC 
AAGAAGAAGT CTGCCCTCTT AGACAGTATT CAGAACCTCC AAGTGGCCTT GACCTCTAAG 
CAGCAGAGCC TGGAGACCGC AATGTCATTC GTGGCCAGGA ATACATTCAA ACGTGTGAGG 
AGTGGCTTCC TGATGAGGAA AGTGGCTGTT TTCTTCAGCA ACAAGCCCAC CAGGGCATCC 
CCCCAGCTCC GAGAGGCCGT ACTCAAGCTT TCCGATGCGG GGATCACACC CTTGTTCCTT 
ACAAGCCAGG AGGACCGACA GCTCATCAAC GCTTTGCAGA TCAATAACAC AGCAGTGGGA 
CATGCCCTGG TTCTGCCTGC AAGGAGGGAT CTCACAGACT TCCTGAAGAA TGTCCTTACC 
TGCCATGTTT GCTTGGACAT TTGCAATATC GACCCATCCT GTGGCTTCGG CAGCTGGAGG 
CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATAGACCT GGCTTTCATC 
TTGGACAGCT CAGAGGCTAC CACACTGTTC CAGTTCAATG AGATGAAGAA ATACATAGGC 
TATGTGATCA GACAGCTGGA CCTGAGCCCA GACCCGAAGG CTTCCCAACA CTTTGCCAGG 
GTGGCTGTTG TGCAGCAGTC AACCTACGAG TCTGTGGACA ATGCCAGTGT GCCGCCTGTG 
AAGGTGGAAT TCTCACTGAC AGACTATGGT GCCAAGGAAA AGCTGTTGGA TTTCCTTAGC 
CGGAGGATGA CCCAACTTCA GGGAACCATG GGCCTGGGCA ATGCCATTGA ATACACTATA 
GAGAACATCT TTGAAAGTGC TCCAAACCCA CGGGACCTCA AAATTATGGT GCTGATGTTG 
ACTGGTGACA TGCAGAGGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT CCAGGCCAAG 
TGCAAGGGTT ACTTCTTTGT GGTTCTGGGC ATTGGCAGGA AGGTGAATAT CAAGGAGGTC 
TACAGCTTTG CCAGTGAGCC CAATGATGTC TTCTTCAAAT TTGTGGACAA ATCAACTGAG 
CTCAACGAGG AGCCTCTGAT GCGCTTTGGG AGGCTGCTCC CATCCTTTGT CAGCAGTGAA 
AATGCATTTT ACCTGCCTCC AGATCTCAGG AAACAGTGTG ATTGGTTCCA AGGGGACCAA 
CCAGTGAAGA ATGGCGTGAA GTTTGGTCAC AAACAGATAA ACCCTCCACA TACTGCTAAT 
TCAAGTCTTA CATCCAAAGT TGTGACCACA ATGAAGCCAG TGACAACGAC CAAACCAACA 
GCAATTGTAA ACCTGCCACC TGCTAAGCCA GCCCCAGCGA GGCCAGCCCC TGCTCAGCCA 
GTTCTTGCTA AGCCAGACCC CGCAAAACCA GCCCAAGCAA GGCCAGCCCC TGCTAAGCCA 
GCTTCTGCTA AGTTGGTGCC TCCTCAGCCA GTCCATGTTC AGCCAGCCCC TGCCCAGACA 
GCTTCTGTAA GACCAGCCCC CGCTAAGCCA GCCCCTCCTC AGCCAGCTGC TGCTAAGCCA 
GTCCCTGCTA AGCCAGCAGT CCCTGCTCAG CCAGCCCCTC CTCAGCCAGC TGCTGCTAAG 
CCAGTCCCTG CTAAGCCAGC AGTCCCTGCT CAGCCAGCCC CTCCTCAGCC AGCTGCTGCT 
AAGCCAGTCC CTGCTAAGCC AGCAGTCCCT GCTCAGCCAG CCGCTGCTCA ACCAATGCCT 
GCTCAGCCAG TCCTTACTAA ATCAGCCGCT GTGAAACCAG CCTCTGCCAA CAAGCCTGTG 
GCAGCCAAAC CTGTGGCCAC GAACACAGCC ACAGCCACAG CCAGACCTGC ATTAGCAGCG 
AAGCCTGCTG CAGCGAAGCC TGCAGCAACG AGACCCCTTG CTGCTGCCAT AAGGCCAGTT 
GCTACCAAGC CAGAGGCCCC CAGGCAACAG GCCAAACCAG CTGCCACCAA GCCAGCCACT 
ACTAAGCCCT TGGCGAGAGT GTCCCGAGAA GTCCAGGTCT CTGAAGTCAC GGAGAACAGT 
GCCAGACTCC ACTGGGAGCG GCCCGAGCCT TCCAGCTCCT TTTTTTATGA CCTCACTGTA 
ACCTCGGCCC ACGACCAGTC CTTGGTTCTG AGACAAAACC TCACTGTCAC TGACCGTGTC 
ATCGGAGGCC TACTGGCTGG ACAGCTGTAC CATGTCGTAG TGGTCTCCTA CCTGCAGTCT 
CAGGTCAGGG CCATCTACCA AGGAAGTTTC AATACGAAGA AAACCCAGCC TCCACCTCTT 
CAGGCAGCAC ACCGAGCATC CAGTTCAACC ATCAACCTCA TGGTGAACAC AGAACCATTG 
TTTCTCACTA AAACAGATAT ATGTAAGCTG TCCAGAGATG CTGGGACTTG TGTGGACTTC 
AAGTTACTAT GGCACTATGA CCTAGAGAGC AAAAGTTGCA AGAGATTCTG GTATGGAGGT 
TGTGGAGGCA ACGAGAACAG ATTCCACTCC CAGGAAGAAT GTGAAAAGAT GTGTAGTCCT 
GAGTTAACAG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a3 ( NM_001243009.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001229938.1 Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant 2, mRNA.

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CloneID	OMu03632
Clone ID Related Accession (Same CDS sequence)	NM_001243009.1
Accession Version	NM_001243009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	collagen alpha-3(VI) chain isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ098732.1, AC158773.6, AK076391.1, CF734596.1, BQ561449.1, BQ560536.1, AC110895.14, FQ976558.1, AA118378.1 and BX638221.1. Transcript Variant: This variant (2) lacks 3 non-consecutive in-frame coding exons compared to variant 1. This results in a shorter isoform (2) missing two internal protein segments compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAAAC ACCGGCATCT GCCCCTAGTG GCTGTCTTTA GCCTTCTCCT CTCAGGCATT 
GCCACGACTC ATGCCCAACA GCATGGAGTC ATGGAAGTCA ACAAGAGGGA CATAGTCTTC 
CTGGTGGATG GTTCCTCCAG CCTGGGGCCA TCCAACTTCA ATGCCATCCG AGACTTCGTT 
ACCAGAGTCA TCCAGAGGTT GGAAATTGGA CAGGACCTGG TTCAGGTATC AGTGGCTCAG 
TATGCAGACA CTGTCAAGCC TGAGTTCTAT TTAAATTCCT ATACCAACAA AAGGGATGCC 
ATAACTGCTG TACGGAAGAT GAGGGCCCTG AATGGCTCAG CCCTGTACAC GGGATCTTCT 
CTGGACTTTG TTCGAAACAA CCTCTTCACT AGCTCAGCCG GCCATAGGGC CGCTGAGGGG 
GTTCCTAAGC TCCTGGTGCT GATCACAGGC GGTAAGTCCC TCGATGAAGT CAGCCAGCCT 
GCCCAGGAGC TGAAGAGAGG CAGCATCATG GCCTTGGCCG TTGGCAGCAA GGCCGCCGAT 
GAGGATGAGC TGAAAGAGAT CGCTTTCGAC TCCTCCCTGG TCTTCATTCC CGCTGAGTTC 
CGCCCTGCTC CCTTGCAAAA CATGCTGCCC AGCCTGATGG CACCTCTCAG GACTCTCACT 
GGAACCACGG AAGAGAGCAA GCGGGACATT CTTTTCCTCT TTGACGGCTC AGTCAATGTC 
TTGGGTCAGT TCCCTGCTGT CAGAGACTTT CTCTACAGGA TCATTGAGGA ACTTGATGTG 
AAGCCGGATG GGACCCGGGT AGCAATAGCT CAGTTCAGTG ATGACGTCAG GCTGGAGTCC 
CGCTTCAGTG AGCACCAGAC GAAAGCTGAA ATCCTTAATC TCGTGAAGAA GATGAAGCTA 
AAAACAGGCA AAGCCCTCAA CCTGGGCTAC GCCCTGGACT ACGCGCTTAG GAACATCTTT 
GTGAGGTCGG CTGGCAGCCG CATCGAGGAT AACGTGCAGC AGTTCCTGGT GCTGCTGGTT 
GCTGGAAGGT CGTCAGATGC TGTGGCCGGG CCTGCAAGCT CCCTGAAGCA GAGAGGAGTG 
GTACCTTTCA TCTTTCAAGC CAAGAACGCC AACCCCAGTG AGCTGGAGCA GATTGTGCTG 
TCGCCTGCAT TCATCCTGGC TGCAGAGTCA CTCCCTAAGA TCGGAGACCT CCAATCACAG 
ATTGTGAGCC TTCTGAAAGC AGAACAGGGT TCAGGCCCTG TATCAGGTGA AAAGGATGTG 
GTGTTTCTCA TTGATGGCTC TGAGGGTGTC CGCAGTGGCT TCCCCCTGCT AAAGGACTTT 
GTGCAGAGGG TTGTGGAGAG CTTGGATGTG GGTCCCGACC GTGTGCGTGT GGCACTGGTG 
CAGTACAGTG ACCGGACCAG GCCGGAGTTC TACCTGAATT CCCACATGGA CCAGCAGGGT 
GTCATCAGCG CCATCCGCAG GCTGACACTG CTGGGCGGCC CAACCCCCAA CACAGGGGCG 
GCACTGGAGT TCGTGTTAAG GAACATTCTG ACCAGTTCTA CTGGGAGCAG GATAGCAGAA 
GGTGTTCCTC AGCTCCTGAT TGTCCTCACA GCAGAGCCAT CAGGGGATGA CGTGCGAGGC 
CCTTCAGTAG TCCTGAAGCA GGGCGGGGCT GTGCCCATTG GCATTGGCAT TGGGAATGCT 
GACATCTCTG AAATGCAGAC CATCTCCTTC ATCCCTGACT TTGCTGTGGC CATCCCCACC 
TTCCGGGAGC TTGGGACGAT ACAGCAGGTC ATCTCTGAGA GGGTGATCCA GCTTAATCGT 
GAGGAGCTGA GCTCGTTGAA ACCCATTTTG ACGCCCTCAA CAGGTGCAGG TGTTGGCAGC 
AAGAAAGATG TGGTCTTTCT CATCGATGGA TCCCGAAATG CTGGCCCTGA GTTCCAGTAC 
ATCCGTACCC TGATTGAGAG GATAGTCGAG TACCTGGACA TAGGCTTTGA CACCACCCGG 
GTAGCCGTCA TCCAGTTCAG TGAGGACTCC AAGATGGAGT TTCCTCTGAA TGCCCACTTC 
AGCAAGGATG AAGTACAGAA CGCTGTGCGC AGGCTGCGGC CCAAGGGTGG GAGTCAGGTC 
TACATCGGAA ATGCCCTGGA GTACGTGCTG AAGAACATCT TCCAGAGGCC ACTGGGGAGC 
CGGATAGAAG AGGGCGTTCC GCAGTTCCTA GTCCTCATTT CGTCTGGAAA GTCTGATGAT 
GAGGTGGACG ACTCAGCCGT GGAACTCAAG CAGTTCGGTG TGGCCCCTCT CACCATAGCC 
AGACACACAG ACCAAGAGGA GCTGGTGAAG ATCTCCCTAA GTCCTGAGTA CGTGTACTCC 
GTGAGCACCT TCAGGGAGCT GCCCAGACTG GAGCAGAAGC TGCTGACGCC CATCACCACT 
CTAACCTCAC AGCAGATCCA TCAGATCCTG GCCAGTACCC GATATCCCCC TTCAGTGGTT 
GAAAGCGATG CGGCTGACAT CGTCTTTCTG ATTGACAGCT CTGATGCCGT CAAGCCCGAT 
GGCATCGCTC ATATCCGAGA CTTTGTCAGC AGGATCGTTC GCAGACTCAA CATTGGTCCC 
AGTAAAGTGA GGATTGGGGT CGTGCAGTTT AGCAATGATG TCTTCCCTGA GTTCTACCTG 
AAGACCCACA AGTCCCAGAG CAGTGTGCTT GAAGCCATAC GGCGTCTGAG GTTCAAAGGA 
GGGTCTCCCC TAAACACTGG CAGAGCCCTG GAGTTTGTGG CAAGAAACCT CTTCGTGAAG 
TCTGCTGGGA GCCGGATAGA AGATGGGGTC CCTCAACACC TGGTCTTATT CCTGGGTGGG 
AAGTCTCAGG ACGATGTGGC CAGGCATGCC CAAGTCATAA GCTCATCGGG GATTGTGAGC 
TTAGGAATAG GAGATCGGAA CATTGATAGG ACGGACTTGC AAACCATCAC CAATGACCCC 
AGGCTGGTCT TCACAGTTCG GGAATTCAGA GAGCTGCCCA ACATAGAAGA GAGGGTCATG 
CTTTCCTTTG GACCCTCTGG GGCTACTCCT CAGCCTCCTG GGGTAGATTT ACCTTCTCCT 
TCCCGGCCAG AGAAGAAGAA GGCCGACATT GTGTTCCTGC TGGATGGTTC CATCAATTTT 
AGGAGGGACA GTTTCCAGGA AGTGCTCCGT TTTGCATCTG AAATCGTGGA CACGGTCTAT 
GAAGACGGAG ATTCCATCAG AGTGGGACTG GTCCAGTACA ACTCAGACCC CACGGATGAA 
TTCTTCCTGA GGGACTTTTC TACCAAAAGG CAGATTATCG ATGCCATCAA CAAAGTCGTC 
TATAAAGGAG GGAGGCATGC CAACACCAGG GTGGGCATCG AGCACCTGCT GCGGAATCAC 
TTTGTGCCGG AGGCTGGTAG CCGCCTGGAT GAGCGGGTTC CTCAGATCGC CTTCGTGATC 
ACCGGAGGGA AGTCAGTGGA GGATGCTCAG GATGTGAGCC TGGCTCTCAC CCAGAAAGGT 
GTCAAGGTGT TTGCCGTTGG CGTGAGGAAC ATCGACTCTG AGGAGGTGGG CAAGATAGCC 
TCCAACAGTG CCACAGCCTT CCGGGTGGGC AGCGTGCAGG AACTGTCCGA ACTGAGTGAG 
ACAGTGCTCG AGACTCTGCA TGATGCAATG CATGAGACCC TCTGCCCTGG TGTGACCGAT 
GTCTCCAAAG CCTGCAATCT GGAAGTCATT CTGGGGTTTG ATGGATCTCG AGATCAGAAT 
GTGTTTGTGA GTCAGAAGGG CCTTGAGTCC AAGGTGGACA TCATCTTGAA CAGAATCAGC 
CAGATACAAA GGATCAGCTG CAGTGGCAAC CAGCTGCCCA CTGTGAGGGT ATCCGTGATG 
GCGAACACGC CCTCTGGGCC AGTGGAGGCC TTTGACTTTG CCGAGTATCA GCCGGAGCTG 
TTTGAGAAGT TTCGCAACAT GCGCAGTCAG CGCCCATACG TCCTCACAGC TGACACGCTG 
AAGTTGTACC AGAACAAGTT CAGGCAGTCG TCGCCTGATA CTGTGAAGGT AGTCATTCAC 
TTCACGGATG GAGCAGATGG AGACATGGCT GACTTATACA GAGCATCTGA GGAACTCCGG 
CAAGCAGGTG CCCAAGCCCT GATCCTGGTG GGTCTTGAAC GTGTGGCTAA CCTGGAGCGG 
CTGATGCATC TGGAGTTCGG GCGGGGCTTC ATGTATGACA GGCCCCTGAG GCTCAACCTG 
CTTGACTTAG ACTATGAACT GGCAGAGCAG CTTGACAATA TTGCTGAGAA AGCTTGCTGT 
GGGGTTCCAT GCAAGTGCTC TGGAGAGAGG GGAGACAGAG GGCCCATTGG CAGCATTGGG 
CCAAAGGGTA TCTCCGGGGA AGATGGCTAC CGAGGCTACC CGGGAGACGA AGGCGGACCT 
GGTGAGCGAG GTCCACCTGG TGTGAATGGC ACACAAGGTT TCCAGGGCTG TCCAGGCCAG 
AGGGGAGTGA AGGGTTCTCG AGGATTCCCA GGAGAGAAGG GTGAATTGGG AGAAATCGGC 
TTGGATGGTC TGGATGGTGA AGAGGGAGAC AAGGGGCTGC CTGGTTCTTC TGGAGAAAAG 
GGGAGTCCTG GAAGAAGGGG TGACAAAGGA CCCAAAGGAG ACAAAGGCGA GAGAGGAGAT 
GTCGGGATTC GAGGTGACCC GGGTGACTCA GGACGGGACA GCCAGCAGAG AGGACCCAAA 
GGAGAGACGG GAGACATCGG CCCCATGGGT CTCCCAGGCA GAGATGGCAT CCCTGGCAGC 
CCAGGAGACC CTGGGAAGGA CGGTGGCTCT GGCCGAAGGG GCCCTGCAGG AGCTAAGGGC 
AACAGAGGTG GTCCTGGCCA GCCAGGCTTC GAAGGCGAGC AGGGTACCAG AGGCTCACAG 
GGCCCACCTG GTCCCATTGG TCCCCCAGGC CTGATTGGGG AACAAGGAAT TCCCGGACCT 
CGAGGAGGCG GAGGTACGGC TGGGGCTCCT GGAGAACGTG GCAGAACTGG TCCTCTGGGG 
AGGAAGGGTG AGCCAGGAGA GCCAGGGCCG AAGGGAAGCA TCGGGAACCG TGGACCCCGA 
GGAGAGACGG GAGATGACGG GAGAGATGGG GTTGGCAGTG AAGGACGCAG AGGCAAAAAA 
GGAGAAAGAG GCTTCCCTGG GTATCCGGGA CCAAAGGGCA CCCCTGGTGA GCCAGGGGCA 
GATGGACCAC CAGGACCCAA AGGCATCAGA GGTCGAAGGG GAAATTCAGG ACCTCCAGGG 
GCAACTGGAC AAAAGGGAGA CCCTGGTTAC CCAGGACCAT CTGGTCACAA GGGCAACCGA 
GGCGACTCCG TTGATCAATG CGCCCTCATC CAGAGCATCA GAGACAAATG CCCTTGCTGC 
TACGGGCCCC TGGAGTGCCC TGTATTCCCA ACAGAACTCG CCTTTGCCCT GGATACCTCT 
GAGGGGGTGA CTCAGGACAC CTTCAGCCGG ATGCGAGAGG TGCTTCTGGG CATTGTGGGC 
GATCTGACCA TTGCAGAGAG CAACTGTCCG CGGGGTGCCC GCGTGGCTGT GGTCACCTAT 
AACAATGAGG TGACTACAGA GATCCGGTTT GCTGACTCCA AGAAGAAGTC TGCCCTCTTA 
GACAGTATTC AGAACCTCCA AGTGGCCTTG ACCTCTAAGC AGCAGAGCCT GGAGACCGCA 
ATGTCATTCG TGGCCAGGAA TACATTCAAA CGTGTGAGGA GTGGCTTCCT GATGAGGAAA 
GTGGCTGTTT TCTTCAGCAA CAAGCCCACC AGGGCATCCC CCCAGCTCCG AGAGGCCGTA 
CTCAAGCTTT CCGATGCGGG GATCACACCC TTGTTCCTTA CAAGCCAGGA GGACCGACAG 
CTCATCAACG CTTTGCAGAT CAATAACACA GCAGTGGGAC ATGCCCTGGT TCTGCCTGCA 
AGGAGGGATC TCACAGACTT CCTGAAGAAT GTCCTTACCT GCCATGTTTG CTTGGACATT 
TGCAATATCG ACCCATCCTG TGGCTTCGGC AGCTGGAGGC CTTCCTTCAG GGACCGGAGG 
GCAGCAGGCA GTGATGTGGA CATAGACCTG GCTTTCATCT TGGACAGCTC AGAGGCTACC 
ACACTGTTCC AGTTCAATGA GATGAAGAAA TACATAGGCT ATGTGATCAG ACAGCTGGAC 
CTGAGCCCAG ACCCGAAGGC TTCCCAACAC TTTGCCAGGG TGGCTGTTGT GCAGCAGTCA 
ACCTACGAGT CTGTGGACAA TGCCAGTGTG CCGCCTGTGA AGGTGGAATT CTCACTGACA 
GACTATGGTG CCAAGGAAAA GCTGTTGGAT TTCCTTAGCC GGAGGATGAC CCAACTTCAG 
GGAACCATGG GCCTGGGCAA TGCCATTGAA TACACTATAG AGAACATCTT TGAAAGTGCT 
CCAAACCCAC GGGACCTCAA AATTATGGTG CTGATGTTGA CTGGTGACAT GCAGAGGCAG 
CAGCTGGAGG AGGCCCAGAG AGCCATCCTC CAGGCCAAGT GCAAGGGTTA CTTCTTTGTG 
GTTCTGGGCA TTGGCAGGAA GGTGAATATC AAGGAGGTCT ACAGCTTTGC CAGTGAGCCC 
AATGATGTCT TCTTCAAATT TGTGGACAAA TCAACTGAGC TCAACGAGGA GCCTCTGATG 
CGCTTTGGGA GGCTGCTCCC ATCCTTTGTC AGCAGTGAAA ATGCATTTTA CCTGCCTCCA 
GATCTCAGGA AACAGTGTGA TTGGTTCCAA GGGGACCAAC CAGTGAAGAA TGGCGTGAAG 
TTTGGTCACA AACAGATAAA CCCTCCACAT ACTGCTAATT CAAGTCTTAC ATCCAAAGTT 
GTGACCACAA TGAAGCCAGT GACAACGACC AAACCAACAG CAATTGTAAA CCTGCCACCT 
GCTAAGCCAG CCCCAGCGAG GCCAGCCCCT GCTCAGCCAG TTCTTGCTAA GCCAGACCCC 
GCAAAACCAG CCCAAGCAAG GCCAGCCCCT GCTAAGCCAG CTTCTGCTAA GTTGGTGCCT 
CCTCAGCCAG TCCATGTTCA GCCAGCCCCT GCCCAGACAG CTTCTGTAAG ACCAGCCCCC 
GCTAAGCCAG CCCCTCCTCA GCCAGCTGCT GCTAAGCCAG TCCCTGCTAA GCCAGCAGTC 
CCTGCTCAGC CAGCCCCTCC TCAGCCAGCT GCTGCTAAGC CAGTCCCTGC TAAGCCAGCA 
GTCCCTGCTC AGCCAGCCCC TCCTCAGCCA GCTGCTGCTA AGCCAGTCCC TGCTAAGCCA 
GCAGTCCCTG CTCAGCCAGC CGCTGCTCAA CCAATGCCTG CTCAGCCAGT CCTTACTAAA 
TCAGCCGCTG TGAAACCAGC CTCTGCCAAC AAGCCTGTGG CAGCCAAACC TGTGGCCACG 
AACACAGCCA CAGCCACAGC CAGACCTGCA TTAGCAGCGA AGCCTGCTGC AGCGAAGCCT 
GCAGCAACGA GACCCCTTGC TGCTGCCATA AGGCCAGTTG CTACCAAGCC AGAGGCCCCC 
AGGCAACAGG CCAAACCAGC TGCCACCAAG CCAGCCACTA CTAAGCCCTT GGCGAGAGTG 
TCCCGAGAAG TCCAGGTCTC TGAAGTCACG GAGAACAGTG CCAGACTCCA CTGGGAGCGG 
CCCGAGCCTT CCAGCTCCTT TTTTTATGAC CTCACTGTAA CCTCGGCCCA CGACCAGTCC 
TTGGTTCTGA GACAAAACCT CACTGTCACT GACCGTGTCA TCGGAGGCCT ACTGGCTGGA 
CAGCTGTACC ATGTCGTAGT GGTCTCCTAC CTGCAGTCTC AGGTCAGGGC CATCTACCAA 
GGAAGTTTCA ATACGAAGAA AACCCAGCCT CCACCTCTTC AGGCAGCACA CCGAGCATCC 
AGTTCAACCA TCAACCTCAT GGTGAACACA GAACCATTGT TTCTCACTAA AACAGATATA 
TGTAAGCTGT CCAGAGATGC TGGGACTTGT GTGGACTTCA AGTTACTATG GCACTATGAC 
CTAGAGAGCA AAAGTTGCAA GAGATTCTGG TATGGAGGTT GTGGAGGCAA CGAGAACAGA 
TTCCACTCCC AGGAAGAATG TGAAAAGATG TGTAGTCCTG AGTTAACAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229938.1
CDS	255..8288
Translation	MRKHRHLPLVAVFSLLLSGIATTHAQQHGVMEVNKRDIVFLVDGSSSLGPSNFNAIRDFVTRVIQRLEIGQDLVQVSVAQYADTVKPEFYLNSYTNKRDAITAVRKMRALNGSALYTGSSLDFVRNNLFTSSAGHRAAEGVPKLLVLITGGKSLDEVSQPAQELKRGSIMALAVGSKAADEDELKEIAFDSSLVFIPAEFRPAPLQNMLPSLMAPLRTLTGTTEESKRDILFLFDGSVNVLGQFPAVRDFLYRIIEELDVKPDGTRVAIAQFSDDVRLESRFSEHQTKAEILNLVKKMKLKTGKALNLGYALDYALRNIFVRSAGSRIEDNVQQFLVLLVAGRSSDAVAGPASSLKQRGVVPFIFQAKNANPSELEQIVLSPAFILAAESLPKIGDLQSQIVSLLKAEQGSGPVSGEKDVVFLIDGSEGVRSGFPLLKDFVQRVVESLDVGPDRVRVALVQYSDRTRPEFYLNSHMDQQGVISAIRRLTLLGGPTPNTGAALEFVLRNILTSSTGSRIAEGVPQLLIVLTAEPSGDDVRGPSVVLKQGGAVPIGIGIGNADISEMQTISFIPDFAVAIPTFRELGTIQQVISERVIQLNREELSSLKPILTPSTGAGVGSKKDVVFLIDGSRNAGPEFQYIRTLIERIVEYLDIGFDTTRVAVIQFSEDSKMEFPLNAHFSKDEVQNAVRRLRPKGGSQVYIGNALEYVLKNIFQRPLGSRIEEGVPQFLVLISSGKSDDEVDDSAVELKQFGVAPLTIARHTDQEELVKISLSPEYVYSVSTFRELPRLEQKLLTPITTLTSQQIHQILASTRYPPSVVESDAADIVFLIDSSDAVKPDGIAHIRDFVSRIVRRLNIGPSKVRIGVVQFSNDVFPEFYLKTHKSQSSVLEAIRRLRFKGGSPLNTGRALEFVARNLFVKSAGSRIEDGVPQHLVLFLGGKSQDDVARHAQVISSSGIVSLGIGDRNIDRTDLQTITNDPRLVFTVREFRELPNIEERVMLSFGPSGATPQPPGVDLPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFASEIVDTVYEDGDSIRVGLVQYNSDPTDEFFLRDFSTKRQIIDAINKVVYKGGRHANTRVGIEHLLRNHFVPEAGSRLDERVPQIAFVITGGKSVEDAQDVSLALTQKGVKVFAVGVRNIDSEEVGKIASNSATAFRVGSVQELSELSETVLETLHDAMHETLCPGVTDVSKACNLEVILGFDGSRDQNVFVSQKGLESKVDIILNRISQIQRISCSGNQLPTVRVSVMANTPSGPVEAFDFAEYQPELFEKFRNMRSQRPYVLTADTLKLYQNKFRQSSPDTVKVVIHFTDGADGDMADLYRASEELRQAGAQALILVGLERVANLERLMHLEFGRGFMYDRPLRLNLLDLDYELAEQLDNIAEKACCGVPCKCSGERGDRGPIGSIGPKGISGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEEGDKGLPGSSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGDSGRDSQQRGPKGETGDIGPMGLPGRDGIPGSPGDPGKDGGSGRRGPAGAKGNRGGPGQPGFEGEQGTRGSQGPPGPIGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGEPGPKGSIGNRGPRGETGDDGRDGVGSEGRRGKKGERGFPGYPGPKGTPGEPGADGPPGPKGIRGRRGNSGPPGATGQKGDPGYPGPSGHKGNRGDSVDQCALIQSIRDKCPCCYGPLECPVFPTELAFALDTSEGVTQDTFSRMREVLLGIVGDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSALLDSIQNLQVALTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRASPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPARRDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDLAFILDSSEATTLFQFNEMKKYIGYVIRQLDLSPDPKASQHFARVAVVQQSTYESVDNASVPPVKVEFSLTDYGAKEKLLDFLSRRMTQLQGTMGLGNAIEYTIENIFESAPNPRDLKIMVLMLTGDMQRQQLEEAQRAILQAKCKGYFFVVLGIGRKVNIKEVYSFASEPNDVFFKFVDKSTELNEEPLMRFGRLLPSFVSSENAFYLPPDLRKQCDWFQGDQPVKNGVKFGHKQINPPHTANSSLTSKVVTTMKPVTTTKPTAIVNLPPAKPAPARPAPAQPVLAKPDPAKPAQARPAPAKPASAKLVPPQPVHVQPAPAQTASVRPAPAKPAPPQPAAAKPVPAKPAVPAQPAPPQPAAAKPVPAKPAVPAQPAPPQPAAAKPVPAKPAVPAQPAAAQPMPAQPVLTKSAAVKPASANKPVAAKPVATNTATATARPALAAKPAAAKPAATRPLAAAIRPVATKPEAPRQQAKPAATKPATTKPLARVSREVQVSEVTENSARLHWERPEPSSSFFYDLTVTSAHDQSLVLRQNLTVTDRVIGGLLAGQLYHVVVVSYLQSQVRAIYQGSFNTKKTQPPPLQAAHRASSSTINLMVNTEPLFLTKTDICKLSRDAGTCVDFKLLWHYDLESKSCKRFWYGGCGGNENRFHSQEECEKMCSPELTV

Target ORF information:

RefSeq Version	NM_001243009.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243009.1

ORF Insert Sequence:

ATGAGGAAAC ACCGGCATCT GCCCCTAGTG GCTGTCTTTA GCCTTCTCCT CTCAGGCATT 
GCCACGACTC ATGCCCAACA GCATGGAGTC ATGGAAGTCA ACAAGAGGGA CATAGTCTTC 
CTGGTGGATG GTTCCTCCAG CCTGGGGCCA TCCAACTTCA ATGCCATCCG AGACTTCGTT 
ACCAGAGTCA TCCAGAGGTT GGAAATTGGA CAGGACCTGG TTCAGGTATC AGTGGCTCAG 
TATGCAGACA CTGTCAAGCC TGAGTTCTAT TTAAATTCCT ATACCAACAA AAGGGATGCC 
ATAACTGCTG TACGGAAGAT GAGGGCCCTG AATGGCTCAG CCCTGTACAC GGGATCTTCT 
CTGGACTTTG TTCGAAACAA CCTCTTCACT AGCTCAGCCG GCCATAGGGC CGCTGAGGGG 
GTTCCTAAGC TCCTGGTGCT GATCACAGGC GGTAAGTCCC TCGATGAAGT CAGCCAGCCT 
GCCCAGGAGC TGAAGAGAGG CAGCATCATG GCCTTGGCCG TTGGCAGCAA GGCCGCCGAT 
GAGGATGAGC TGAAAGAGAT CGCTTTCGAC TCCTCCCTGG TCTTCATTCC CGCTGAGTTC 
CGCCCTGCTC CCTTGCAAAA CATGCTGCCC AGCCTGATGG CACCTCTCAG GACTCTCACT 
GGAACCACGG AAGAGAGCAA GCGGGACATT CTTTTCCTCT TTGACGGCTC AGTCAATGTC 
TTGGGTCAGT TCCCTGCTGT CAGAGACTTT CTCTACAGGA TCATTGAGGA ACTTGATGTG 
AAGCCGGATG GGACCCGGGT AGCAATAGCT CAGTTCAGTG ATGACGTCAG GCTGGAGTCC 
CGCTTCAGTG AGCACCAGAC GAAAGCTGAA ATCCTTAATC TCGTGAAGAA GATGAAGCTA 
AAAACAGGCA AAGCCCTCAA CCTGGGCTAC GCCCTGGACT ACGCGCTTAG GAACATCTTT 
GTGAGGTCGG CTGGCAGCCG CATCGAGGAT AACGTGCAGC AGTTCCTGGT GCTGCTGGTT 
GCTGGAAGGT CGTCAGATGC TGTGGCCGGG CCTGCAAGCT CCCTGAAGCA GAGAGGAGTG 
GTACCTTTCA TCTTTCAAGC CAAGAACGCC AACCCCAGTG AGCTGGAGCA GATTGTGCTG 
TCGCCTGCAT TCATCCTGGC TGCAGAGTCA CTCCCTAAGA TCGGAGACCT CCAATCACAG 
ATTGTGAGCC TTCTGAAAGC AGAACAGGGT TCAGGCCCTG TATCAGGTGA AAAGGATGTG 
GTGTTTCTCA TTGATGGCTC TGAGGGTGTC CGCAGTGGCT TCCCCCTGCT AAAGGACTTT 
GTGCAGAGGG TTGTGGAGAG CTTGGATGTG GGTCCCGACC GTGTGCGTGT GGCACTGGTG 
CAGTACAGTG ACCGGACCAG GCCGGAGTTC TACCTGAATT CCCACATGGA CCAGCAGGGT 
GTCATCAGCG CCATCCGCAG GCTGACACTG CTGGGCGGCC CAACCCCCAA CACAGGGGCG 
GCACTGGAGT TCGTGTTAAG GAACATTCTG ACCAGTTCTA CTGGGAGCAG GATAGCAGAA 
GGTGTTCCTC AGCTCCTGAT TGTCCTCACA GCAGAGCCAT CAGGGGATGA CGTGCGAGGC 
CCTTCAGTAG TCCTGAAGCA GGGCGGGGCT GTGCCCATTG GCATTGGCAT TGGGAATGCT 
GACATCTCTG AAATGCAGAC CATCTCCTTC ATCCCTGACT TTGCTGTGGC CATCCCCACC 
TTCCGGGAGC TTGGGACGAT ACAGCAGGTC ATCTCTGAGA GGGTGATCCA GCTTAATCGT 
GAGGAGCTGA GCTCGTTGAA ACCCATTTTG ACGCCCTCAA CAGGTGCAGG TGTTGGCAGC 
AAGAAAGATG TGGTCTTTCT CATCGATGGA TCCCGAAATG CTGGCCCTGA GTTCCAGTAC 
ATCCGTACCC TGATTGAGAG GATAGTCGAG TACCTGGACA TAGGCTTTGA CACCACCCGG 
GTAGCCGTCA TCCAGTTCAG TGAGGACTCC AAGATGGAGT TTCCTCTGAA TGCCCACTTC 
AGCAAGGATG AAGTACAGAA CGCTGTGCGC AGGCTGCGGC CCAAGGGTGG GAGTCAGGTC 
TACATCGGAA ATGCCCTGGA GTACGTGCTG AAGAACATCT TCCAGAGGCC ACTGGGGAGC 
CGGATAGAAG AGGGCGTTCC GCAGTTCCTA GTCCTCATTT CGTCTGGAAA GTCTGATGAT 
GAGGTGGACG ACTCAGCCGT GGAACTCAAG CAGTTCGGTG TGGCCCCTCT CACCATAGCC 
AGACACACAG ACCAAGAGGA GCTGGTGAAG ATCTCCCTAA GTCCTGAGTA CGTGTACTCC 
GTGAGCACCT TCAGGGAGCT GCCCAGACTG GAGCAGAAGC TGCTGACGCC CATCACCACT 
CTAACCTCAC AGCAGATCCA TCAGATCCTG GCCAGTACCC GATATCCCCC TTCAGTGGTT 
GAAAGCGATG CGGCTGACAT CGTCTTTCTG ATTGACAGCT CTGATGCCGT CAAGCCCGAT 
GGCATCGCTC ATATCCGAGA CTTTGTCAGC AGGATCGTTC GCAGACTCAA CATTGGTCCC 
AGTAAAGTGA GGATTGGGGT CGTGCAGTTT AGCAATGATG TCTTCCCTGA GTTCTACCTG 
AAGACCCACA AGTCCCAGAG CAGTGTGCTT GAAGCCATAC GGCGTCTGAG GTTCAAAGGA 
GGGTCTCCCC TAAACACTGG CAGAGCCCTG GAGTTTGTGG CAAGAAACCT CTTCGTGAAG 
TCTGCTGGGA GCCGGATAGA AGATGGGGTC CCTCAACACC TGGTCTTATT CCTGGGTGGG 
AAGTCTCAGG ACGATGTGGC CAGGCATGCC CAAGTCATAA GCTCATCGGG GATTGTGAGC 
TTAGGAATAG GAGATCGGAA CATTGATAGG ACGGACTTGC AAACCATCAC CAATGACCCC 
AGGCTGGTCT TCACAGTTCG GGAATTCAGA GAGCTGCCCA ACATAGAAGA GAGGGTCATG 
CTTTCCTTTG GACCCTCTGG GGCTACTCCT CAGCCTCCTG GGGTAGATTT ACCTTCTCCT 
TCCCGGCCAG AGAAGAAGAA GGCCGACATT GTGTTCCTGC TGGATGGTTC CATCAATTTT 
AGGAGGGACA GTTTCCAGGA AGTGCTCCGT TTTGCATCTG AAATCGTGGA CACGGTCTAT 
GAAGACGGAG ATTCCATCAG AGTGGGACTG GTCCAGTACA ACTCAGACCC CACGGATGAA 
TTCTTCCTGA GGGACTTTTC TACCAAAAGG CAGATTATCG ATGCCATCAA CAAAGTCGTC 
TATAAAGGAG GGAGGCATGC CAACACCAGG GTGGGCATCG AGCACCTGCT GCGGAATCAC 
TTTGTGCCGG AGGCTGGTAG CCGCCTGGAT GAGCGGGTTC CTCAGATCGC CTTCGTGATC 
ACCGGAGGGA AGTCAGTGGA GGATGCTCAG GATGTGAGCC TGGCTCTCAC CCAGAAAGGT 
GTCAAGGTGT TTGCCGTTGG CGTGAGGAAC ATCGACTCTG AGGAGGTGGG CAAGATAGCC 
TCCAACAGTG CCACAGCCTT CCGGGTGGGC AGCGTGCAGG AACTGTCCGA ACTGAGTGAG 
ACAGTGCTCG AGACTCTGCA TGATGCAATG CATGAGACCC TCTGCCCTGG TGTGACCGAT 
GTCTCCAAAG CCTGCAATCT GGAAGTCATT CTGGGGTTTG ATGGATCTCG AGATCAGAAT 
GTGTTTGTGA GTCAGAAGGG CCTTGAGTCC AAGGTGGACA TCATCTTGAA CAGAATCAGC 
CAGATACAAA GGATCAGCTG CAGTGGCAAC CAGCTGCCCA CTGTGAGGGT ATCCGTGATG 
GCGAACACGC CCTCTGGGCC AGTGGAGGCC TTTGACTTTG CCGAGTATCA GCCGGAGCTG 
TTTGAGAAGT TTCGCAACAT GCGCAGTCAG CGCCCATACG TCCTCACAGC TGACACGCTG 
AAGTTGTACC AGAACAAGTT CAGGCAGTCG TCGCCTGATA CTGTGAAGGT AGTCATTCAC 
TTCACGGATG GAGCAGATGG AGACATGGCT GACTTATACA GAGCATCTGA GGAACTCCGG 
CAAGCAGGTG CCCAAGCCCT GATCCTGGTG GGTCTTGAAC GTGTGGCTAA CCTGGAGCGG 
CTGATGCATC TGGAGTTCGG GCGGGGCTTC ATGTATGACA GGCCCCTGAG GCTCAACCTG 
CTTGACTTAG ACTATGAACT GGCAGAGCAG CTTGACAATA TTGCTGAGAA AGCTTGCTGT 
GGGGTTCCAT GCAAGTGCTC TGGAGAGAGG GGAGACAGAG GGCCCATTGG CAGCATTGGG 
CCAAAGGGTA TCTCCGGGGA AGATGGCTAC CGAGGCTACC CGGGAGACGA AGGCGGACCT 
GGTGAGCGAG GTCCACCTGG TGTGAATGGC ACACAAGGTT TCCAGGGCTG TCCAGGCCAG 
AGGGGAGTGA AGGGTTCTCG AGGATTCCCA GGAGAGAAGG GTGAATTGGG AGAAATCGGC 
TTGGATGGTC TGGATGGTGA AGAGGGAGAC AAGGGGCTGC CTGGTTCTTC TGGAGAAAAG 
GGGAGTCCTG GAAGAAGGGG TGACAAAGGA CCCAAAGGAG ACAAAGGCGA GAGAGGAGAT 
GTCGGGATTC GAGGTGACCC GGGTGACTCA GGACGGGACA GCCAGCAGAG AGGACCCAAA 
GGAGAGACGG GAGACATCGG CCCCATGGGT CTCCCAGGCA GAGATGGCAT CCCTGGCAGC 
CCAGGAGACC CTGGGAAGGA CGGTGGCTCT GGCCGAAGGG GCCCTGCAGG AGCTAAGGGC 
AACAGAGGTG GTCCTGGCCA GCCAGGCTTC GAAGGCGAGC AGGGTACCAG AGGCTCACAG 
GGCCCACCTG GTCCCATTGG TCCCCCAGGC CTGATTGGGG AACAAGGAAT TCCCGGACCT 
CGAGGAGGCG GAGGTACGGC TGGGGCTCCT GGAGAACGTG GCAGAACTGG TCCTCTGGGG 
AGGAAGGGTG AGCCAGGAGA GCCAGGGCCG AAGGGAAGCA TCGGGAACCG TGGACCCCGA 
GGAGAGACGG GAGATGACGG GAGAGATGGG GTTGGCAGTG AAGGACGCAG AGGCAAAAAA 
GGAGAAAGAG GCTTCCCTGG GTATCCGGGA CCAAAGGGCA CCCCTGGTGA GCCAGGGGCA 
GATGGACCAC CAGGACCCAA AGGCATCAGA GGTCGAAGGG GAAATTCAGG ACCTCCAGGG 
GCAACTGGAC AAAAGGGAGA CCCTGGTTAC CCAGGACCAT CTGGTCACAA GGGCAACCGA 
GGCGACTCCG TTGATCAATG CGCCCTCATC CAGAGCATCA GAGACAAATG CCCTTGCTGC 
TACGGGCCCC TGGAGTGCCC TGTATTCCCA ACAGAACTCG CCTTTGCCCT GGATACCTCT 
GAGGGGGTGA CTCAGGACAC CTTCAGCCGG ATGCGAGAGG TGCTTCTGGG CATTGTGGGC 
GATCTGACCA TTGCAGAGAG CAACTGTCCG CGGGGTGCCC GCGTGGCTGT GGTCACCTAT 
AACAATGAGG TGACTACAGA GATCCGGTTT GCTGACTCCA AGAAGAAGTC TGCCCTCTTA 
GACAGTATTC AGAACCTCCA AGTGGCCTTG ACCTCTAAGC AGCAGAGCCT GGAGACCGCA 
ATGTCATTCG TGGCCAGGAA TACATTCAAA CGTGTGAGGA GTGGCTTCCT GATGAGGAAA 
GTGGCTGTTT TCTTCAGCAA CAAGCCCACC AGGGCATCCC CCCAGCTCCG AGAGGCCGTA 
CTCAAGCTTT CCGATGCGGG GATCACACCC TTGTTCCTTA CAAGCCAGGA GGACCGACAG 
CTCATCAACG CTTTGCAGAT CAATAACACA GCAGTGGGAC ATGCCCTGGT TCTGCCTGCA 
AGGAGGGATC TCACAGACTT CCTGAAGAAT GTCCTTACCT GCCATGTTTG CTTGGACATT 
TGCAATATCG ACCCATCCTG TGGCTTCGGC AGCTGGAGGC CTTCCTTCAG GGACCGGAGG 
GCAGCAGGCA GTGATGTGGA CATAGACCTG GCTTTCATCT TGGACAGCTC AGAGGCTACC 
ACACTGTTCC AGTTCAATGA GATGAAGAAA TACATAGGCT ATGTGATCAG ACAGCTGGAC 
CTGAGCCCAG ACCCGAAGGC TTCCCAACAC TTTGCCAGGG TGGCTGTTGT GCAGCAGTCA 
ACCTACGAGT CTGTGGACAA TGCCAGTGTG CCGCCTGTGA AGGTGGAATT CTCACTGACA 
GACTATGGTG CCAAGGAAAA GCTGTTGGAT TTCCTTAGCC GGAGGATGAC CCAACTTCAG 
GGAACCATGG GCCTGGGCAA TGCCATTGAA TACACTATAG AGAACATCTT TGAAAGTGCT 
CCAAACCCAC GGGACCTCAA AATTATGGTG CTGATGTTGA CTGGTGACAT GCAGAGGCAG 
CAGCTGGAGG AGGCCCAGAG AGCCATCCTC CAGGCCAAGT GCAAGGGTTA CTTCTTTGTG 
GTTCTGGGCA TTGGCAGGAA GGTGAATATC AAGGAGGTCT ACAGCTTTGC CAGTGAGCCC 
AATGATGTCT TCTTCAAATT TGTGGACAAA TCAACTGAGC TCAACGAGGA GCCTCTGATG 
CGCTTTGGGA GGCTGCTCCC ATCCTTTGTC AGCAGTGAAA ATGCATTTTA CCTGCCTCCA 
GATCTCAGGA AACAGTGTGA TTGGTTCCAA GGGGACCAAC CAGTGAAGAA TGGCGTGAAG 
TTTGGTCACA AACAGATAAA CCCTCCACAT ACTGCTAATT CAAGTCTTAC ATCCAAAGTT 
GTGACCACAA TGAAGCCAGT GACAACGACC AAACCAACAG CAATTGTAAA CCTGCCACCT 
GCTAAGCCAG CCCCAGCGAG GCCAGCCCCT GCTCAGCCAG TTCTTGCTAA GCCAGACCCC 
GCAAAACCAG CCCAAGCAAG GCCAGCCCCT GCTAAGCCAG CTTCTGCTAA GTTGGTGCCT 
CCTCAGCCAG TCCATGTTCA GCCAGCCCCT GCCCAGACAG CTTCTGTAAG ACCAGCCCCC 
GCTAAGCCAG CCCCTCCTCA GCCAGCTGCT GCTAAGCCAG TCCCTGCTAA GCCAGCAGTC 
CCTGCTCAGC CAGCCCCTCC TCAGCCAGCT GCTGCTAAGC CAGTCCCTGC TAAGCCAGCA 
GTCCCTGCTC AGCCAGCCCC TCCTCAGCCA GCTGCTGCTA AGCCAGTCCC TGCTAAGCCA 
GCAGTCCCTG CTCAGCCAGC CGCTGCTCAA CCAATGCCTG CTCAGCCAGT CCTTACTAAA 
TCAGCCGCTG TGAAACCAGC CTCTGCCAAC AAGCCTGTGG CAGCCAAACC TGTGGCCACG 
AACACAGCCA CAGCCACAGC CAGACCTGCA TTAGCAGCGA AGCCTGCTGC AGCGAAGCCT 
GCAGCAACGA GACCCCTTGC TGCTGCCATA AGGCCAGTTG CTACCAAGCC AGAGGCCCCC 
AGGCAACAGG CCAAACCAGC TGCCACCAAG CCAGCCACTA CTAAGCCCTT GGCGAGAGTG 
TCCCGAGAAG TCCAGGTCTC TGAAGTCACG GAGAACAGTG CCAGACTCCA CTGGGAGCGG 
CCCGAGCCTT CCAGCTCCTT TTTTTATGAC CTCACTGTAA CCTCGGCCCA CGACCAGTCC 
TTGGTTCTGA GACAAAACCT CACTGTCACT GACCGTGTCA TCGGAGGCCT ACTGGCTGGA 
CAGCTGTACC ATGTCGTAGT GGTCTCCTAC CTGCAGTCTC AGGTCAGGGC CATCTACCAA 
GGAAGTTTCA ATACGAAGAA AACCCAGCCT CCACCTCTTC AGGCAGCACA CCGAGCATCC 
AGTTCAACCA TCAACCTCAT GGTGAACACA GAACCATTGT TTCTCACTAA AACAGATATA 
TGTAAGCTGT CCAGAGATGC TGGGACTTGT GTGGACTTCA AGTTACTATG GCACTATGAC 
CTAGAGAGCA AAAGTTGCAA GAGATTCTGG TATGGAGGTT GTGGAGGCAA CGAGAACAGA 
TTCCACTCCC AGGAAGAATG TGAAAAGATG TGTAGTCCTG AGTTAACAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a3 ( XM_030245436.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101296.1 Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant X1, mRNA.

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CloneID	OMu84996
Clone ID Related Accession (Same CDS sequence)	XM_030245436.1
Accession Version	XM_030245436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-3(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAAC ACCGGCATCT GCCCCTAGTG GCTGTCTTTA GCCTTCTCCT CTCAGGCATT 
GCCACGACTC ATGCCCAACA GCATGGAGAT GTCAAAAACG GTGCGGCGGC TGACATAGTG 
TTTCTAGTGG ATTCCTCTTG GAGTGCTGGG AAGGACCGTT TCCTGCTTGT TCAAGAGTTT 
CTGTCTGATG TTGTAGAGTC TTTAGCTGTG GGAGACAATG ATTTTCACTT TGCTCTGGTC 
CGGCTCAACG GAAACCCACA TACCGAGTTC CTGTTAAATA CATACCACTC TAAACAAGAA 
GTCCTTTCTC ATATTGTGAA CATGTCTTAC ATTGGGGGAA GCAACCAAAC TGGAAAAGGA 
TTAGAATACG TAATTCACAG CCACCTCACT GAGGCTTCCG GAAGCCGGGC TGCTGACGGA 
GTCCCTCAGG TTATCATAGT ATTAACTGAT GGACAATCGG AGGATGGCTT CGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC GGACGTTAAT GTGTTTGCAG TCGGAGTTGA GGGTGCAGAT 
GAAAGAGCCC TAGGAGAAGT AGCAAGCGAA CCACTCAGTA TGCATGTTTT CAACCTAGAG 
AACGTTACCT CACTGCATGG CCTAGTAGGG AACTTAGTGT CCTGTATACA CTCCTCTGTG 
AATCCAGAAA GGGCTGGGGA CAAGGAAAGC CTTAAAGACA TCACAGCACA AGACTCAGCT 
GACATTATTT TCCTTATTGA TGGATCACAG AACACCGGAA ATGCCAATTT CGATGTCATT 
CGAGACTTCC TTGTAAATGT CCTTGAGAGA CTCTCAGTTG GAAACCAGCA GGTCCAAGTG 
GGGGTGGTAC AGTATAGCGA GGAGCCCATA ACCATGTTCT CCTTGAATAG CTACCCCTCC 
AAGGCTGCAG TTCTGGATGC CGTGAAAGGG CTCAGCTTGG TTGGGGGGGA GTCGGCCAAT 
ATAGGCCAGG CTCTTGACTT TGTAGTGGAG AATCACTTCA CTCGGGCAGG TGGCAGCCGC 
GTGGAGGAAG GGGTCCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATTCGAGATT CGGTGGTAGC TCTGAAGCAG GCTAGTGTGT TCTCGTTTGG CCTCGGAGCC 
CAGGCAGCCT CCAGGGCAGA GCTTCAGCAC ATAGCTACCG ATGACAGCTT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCTT TGGGGATCTC CAGGAGCAAA TACTGCCGTA CCTTGTTGGT 
GTGGCCCAAA GGCACATTGT CTTGCAACCA CCAGCTATTG TCACACAAGT CATGGAAGTC 
AACAAGAGGG ACATAGTCTT CCTGGTGGAT GGTTCCTCCA GCCTGGGGCC ATCCAACTTC 
AATGCCATCC GAGACTTCGT TACCAGAGTC ATCCAGAGGT TGGAAATTGG ACAGGACCTG 
GTTCAGGTAT CAGTGGCTCA GTATGCAGAC ACTGTCAAGC CTGAGTTCTA TTTAAATTCC 
TATACCAACA AAAGGGATGC CATAACTGCT GTACGGAAGA TGAGGGCCCT GAATGGCTCA 
GCCCTGTACA CGGGATCTTC TCTGGACTTT GTTCGAAACA ACCTCTTCAC TAGCTCAGCC 
GGCCATAGGG CCGCTGAGGG GGTTCCTAAG CTCCTGGTGC TGATCACAGG CGGTAAGTCC 
CTCGATGAAG TCAGCCAGCC TGCCCAGGAG CTGAAGAGAG GCAGCATCAT GGCCTTGGCC 
GTTGGCAGCA AGGCCGCCGA TGAGGATGAG CTGAAAGAGA TCGCTTTCGA CTCCTCCCTG 
GTCTTCATTC CCGCTGAGTT CCGCCCTGCT CCCTTGCAAA ACATGCTGCC CAGCCTGATG 
GCACCTCTCA GGACTCTCAC TGGAACCACG GAAGAGAGCA AGCGGGACAT TCTTTTCCTC 
TTTGACGGCT CAGTCAATGT CTTGGGTCAG TTCCCTGCTG TCAGAGACTT TCTCTACAGG 
ATCATTGAGG AACTTGATGT GAAGCCGGAT GGGACCCGGG TAGCAATAGC TCAGTTCAGT 
GATGACGTCA GGCTGGAGTC CCGCTTCAGT GAGCACCAGA CGAAAGCTGA AATCCTTAAT 
CTCGTGAAGA AGATGAAGCT AAAAACAGGC AAAGCCCTCA ACCTGGGCTA CGCCCTGGAC 
TACGCGCTTA GGAACATCTT TGTGAGGTCG GCTGGCAGCC GCATCGAGGA TAACGTGCAG 
CAGTTCCTGG TGCTGCTGGT TGCTGGAAGG TCGTCAGATG CTGTGGCCGG GCCTGCAAGC 
TCCCTGAAGC AGAGAGGAGT GGTACCTTTC ATCTTTCAAG CCAAGAACGC CAACCCCAGT 
GAGCTGGAGC AGATTGTGCT GTCGCCTGCA TTCATCCTGG CTGCAGAGTC ACTCCCTAAG 
ATCGGAGACC TCCAATCACA GATTGTGAGC CTTCTGAAAG CAGAACAGGG TTCAGGCCCT 
GTATCAGGTG AAAAGGATGT GGTGTTTCTC ATTGATGGCT CTGAGGGTGT CCGCAGTGGC 
TTCCCCCTGC TAAAGGACTT TGTGCAGAGG GTTGTGGAGA GCTTGGATGT GGGTCCCGAC 
CGTGTGCGTG TGGCACTGGT GCAGTACAGT GACCGGACCA GGCCGGAGTT CTACCTGAAT 
TCCCACATGG ACCAGCAGGG TGTCATCAGC GCCATCCGCA GGCTGACACT GCTGGGCGGC 
CCAACCCCCA ACACAGGGGC GGCACTGGAG TTCGTGTTAA GGAACATTCT GACCAGTTCT 
ACTGGGAGCA GGATAGCAGA AGGTGTTCCT CAGCTCCTGA TTGTCCTCAC AGCAGAGCCA 
TCAGGGGATG ACGTGCGAGG CCCTTCAGTA GTCCTGAAGC AGGGCGGGGC TGTGCCCATT 
GGCATTGGCA TTGGGAATGC TGACATCTCT GAAATGCAGA CCATCTCCTT CATCCCTGAC 
TTTGCTGTGG CCATCCCCAC CTTCCGGGAG CTTGGGACGA TACAGCAGGT CATCTCTGAG 
AGGGTGATCC AGCTTAATCG TGAGGAGCTG AGCTCGTTGA AACCCATTTT GACGCCCTCA 
ACAGGTGCAG GTGTTGGCAG CAAGAAAGAT GTGGTCTTTC TCATCGATGG ATCCCGAAAT 
GCTGGCCCTG AGTTCCAGTA CATCCGTACC CTGATTGAGA GGATAGTCGA GTACCTGGAC 
ATAGGCTTTG ACACCACCCG GGTAGCCGTC ATCCAGTTCA GTGAGGACTC CAAGATGGAG 
TTTCCTCTGA ATGCCCACTT CAGCAAGGAT GAAGTACAGA ACGCTGTGCG CAGGCTGCGG 
CCCAAGGGTG GGAGTCAGGT CTACATCGGA AATGCCCTGG AGTACGTGCT GAAGAACATC 
TTCCAGAGGC CACTGGGGAG CCGGATAGAA GAGGGCGTTC CGCAGTTCCT AGTCCTCATT 
TCGTCTGGAA AGTCTGATGA TGAGGTGGAC GACTCAGCCG TGGAACTCAA GCAGTTCGGT 
GTGGCCCCTC TCACCATAGC CAGACACACA GACCAAGAGG AGCTGGTGAA GATCTCCCTA 
AGTCCTGAGT ACGTGTACTC CGTGAGCACC TTCAGGGAGC TGCCCAGACT GGAGCAGAAG 
CTGCTGACGC CCATCACCAC TCTAACCTCA CAGCAGATCC ATCAGATCCT GGCCAGTACC 
CGATATCCCC CTTCAGTGGT TGAAAGCGAT GCGGCTGACA TCGTCTTTCT GATTGACAGC 
TCTGATGCCG TCAAGCCCGA TGGCATCGCT CATATCCGAG ACTTTGTCAG CAGGATCGTT 
CGCAGACTCA ACATTGGTCC CAGTAAAGTG AGGATTGGGG TCGTGCAGTT TAGCAATGAT 
GTCTTCCCTG AGTTCTACCT GAAGACCCAC AAGTCCCAGA GCAGTGTGCT TGAAGCCATA 
CGGCGTCTGA GGTTCAAAGG AGGGTCTCCC CTAAACACTG GCAGAGCCCT GGAGTTTGTG 
GCAAGAAACC TCTTCGTGAA GTCTGCTGGG AGCCGGATAG AAGATGGGGT CCCTCAACAC 
CTGGTCTTAT TCCTGGGTGG GAAGTCTCAG GACGATGTGG CCAGGCATGC CCAAGTCATA 
AGCTCATCGG GGATTGTGAG CTTAGGAATA GGAGATCGGA ACATTGATAG GACGGACTTG 
CAAACCATCA CCAATGACCC CAGGCTGGTC TTCACAGTTC GGGAATTCAG AGAGCTGCCC 
AACATAGAAG AGAGGGTCAT GCTTTCCTTT GGACCCTCTG GGGCTACTCC TCAGCCTCCT 
GGGGTAGATT TACCTTCTCC TTCCCGGCCA GAGAAGAAGA AGGCCGACAT TGTGTTCCTG 
CTGGATGGTT CCATCAATTT TAGGAGGGAC AGTTTCCAGG AAGTGCTCCG TTTTGCATCT 
GAAATCGTGG ACACGGTCTA TGAAGACGGA GATTCCATCA GAGTGGGACT GGTCCAGTAC 
AACTCAGACC CCACGGATGA ATTCTTCCTG AGGGACTTTT CTACCAAAAG GCAGATTATC 
GATGCCATCA ACAAAGTCGT CTATAAAGGA GGGAGGCATG CCAACACCAG GGTGGGCATC 
GAGCACCTGC TGCGGAATCA CTTTGTGCCG GAGGCTGGTA GCCGCCTGGA TGAGCGGGTT 
CCTCAGATCG CCTTCGTGAT CACCGGAGGG AAGTCAGTGG AGGATGCTCA GGATGTGAGC 
CTGGCTCTCA CCCAGAAAGG TGTCAAGGTG TTTGCCGTTG GCGTGAGGAA CATCGACTCT 
GAGGAGGTGG GCAAGATAGC CTCCAACAGT GCCACAGCCT TCCGGGTGGG CAGCGTGCAG 
GAACTGTCCG AACTGAGTGA GACAGTGCTC GAGACTCTGC ATGATGCAAT GCATGAGACC 
CTCTGCCCTG GTGTGACCGA TGTCTCCAAA GCCTGCAATC TGGAAGTCAT TCTGGGGTTT 
GATGGATCTC GAGATCAGAA TGTGTTTGTG AGTCAGAAGG GCCTTGAGTC CAAGGTGGAC 
ATCATCTTGA ACAGAATCAG CCAGATACAA AGGATCAGCT GCAGTGGCAA CCAGCTGCCC 
ACTGTGAGGG TATCCGTGAT GGCGAACACG CCCTCTGGGC CAGTGGAGGC CTTTGACTTT 
GCCGAGTATC AGCCGGAGCT GTTTGAGAAG TTTCGCAACA TGCGCAGTCA GCGCCCATAC 
GTCCTCACAG CTGACACGCT GAAGTTGTAC CAGAACAAGT TCAGGCAGTC GTCGCCTGAT 
ACTGTGAAGG TAGTCATTCA CTTCACGGAT GGAGCAGATG GAGACATGGC TGACTTATAC 
AGAGCATCTG AGGAACTCCG GCAAGCAGGT GCCCAAGCCC TGATCCTGGT GGGTCTTGAA 
CGTGTGGCTA ACCTGGAGCG GCTGATGCAT CTGGAGTTCG GGCGGGGCTT CATGTATGAC 
AGGCCCCTGA GGCTCAACCT GCTTGACTTA GACTATGAAC TGGCAGAGCA GCTTGACAAT 
ATTGCTGAGA AAGCTTGCTG TGGGGTTCCA TGCAAGTGCT CTGGAGAGAG GGGAGACAGA 
GGGCCCATTG GCAGCATTGG GCCAAAGGGT ATCTCCGGGG AAGATGGCTA CCGAGGCTAC 
CCGGGAGACG AAGGCGGACC TGGTGAGCGA GGTCCACCTG GTGTGAATGG CACACAAGGT 
TTCCAGGGCT GTCCAGGCCA GAGGGGAGTG AAGGGTTCTC GAGGATTCCC AGGAGAGAAG 
GGTGAATTGG GAGAAATCGG CTTGGATGGT CTGGATGGTG AAGAGGGAGA CAAGGGGCTG 
CCTGGTTCTT CTGGAGAAAA GGGGAGTCCT GGAAGAAGGG GTGACAAAGG ACCCAAAGGA 
GACAAAGGCG AGAGAGGAGA TGTCGGGATT CGAGGTGACC CGGGTGACTC AGGACGGGAC 
AGCCAGCAGA GAGGACCCAA AGGAGAGACG GGAGACATCG GCCCCATGGG TCTCCCAGGC 
AGAGATGGCA TCCCTGGCAG CCCAGGAGAC CCTGGGAAGG ACGGTGGCTC TGGCCGAAGG 
GGCCCTGCAG GAGCTAAGGG CAACAGAGGT GGTCCTGGCC AGCCAGGCTT CGAAGGCGAG 
CAGGGTACCA GAGGCTCACA GGGCCCACCT GGTCCCATTG GTCCCCCAGG CCTGATTGGG 
GAACAAGGAA TTCCCGGACC TCGAGGAGGC GGAGGTACGG CTGGGGCTCC TGGAGAACGT 
GGCAGAACTG GTCCTCTGGG GAGGAAGGGT GAGCCAGGAG AGCCAGGGCC GAAGGGAAGC 
ATCGGGAACC GTGGACCCCG AGGAGAGACG GGAGATGACG GGAGAGATGG GGTTGGCAGT 
GAAGGACGCA GAGGCAAAAA AGGAGAAAGA GGCTTCCCTG GGTATCCGGG ACCAAAGGGC 
ACCCCTGGTG AGCCAGGGGC AGATGGACCA CCAGGACCCA AAGGCATCAG AGGTCGAAGG 
GGAAATTCAG GACCTCCAGG GGCAACTGGA CAAAAGGGAG ACCCTGGTTA CCCAGGACCA 
TCTGGTCACA AGGGCAACCG AGGCGACTCC GTTGATCAAT GCGCCCTCAT CCAGAGCATC 
AGAGACAAAT GCCCTTGCTG CTACGGGCCC CTGGAGTGCC CTGTATTCCC AACAGAACTC 
GCCTTTGCCC TGGATACCTC TGAGGGGGTG ACTCAGGACA CCTTCAGCCG GATGCGAGAG 
GTGCTTCTGG GCATTGTGGG CGATCTGACC ATTGCAGAGA GCAACTGTCC GCGGGGTGCC 
CGCGTGGCTG TGGTCACCTA TAACAATGAG GTGACTACAG AGATCCGGTT TGCTGACTCC 
AAGAAGAAGT CTGCCCTCTT AGACAGTATT CAGAACCTCC AAGTGGCCTT GACCTCTAAG 
CAGCAGAGCC TGGAGACCGC AATGTCATTC GTGGCCAGGA ATACATTCAA ACGTGTGAGG 
AGTGGCTTCC TGATGAGGAA AGTGGCTGTT TTCTTCAGCA ACAAGCCCAC CAGGGCATCC 
CCCCAGCTCC GAGAGGCCGT ACTCAAGCTT TCCGATGCGG GGATCACACC CTTGTTCCTT 
ACAAGCCAGG AGGACCGACA GCTCATCAAC GCTTTGCAGA TCAATAACAC AGCAGTGGGA 
CATGCCCTGG TTCTGCCTGC AAGGAGGGAT CTCACAGACT TCCTGAAGAA TGTCCTTACC 
TGCCATGTTT GCTTGGACAT TTGCAATATC GACCCATCCT GTGGCTTCGG CAGCTGGAGG 
CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATAGACCT GGCTTTCATC 
TTGGACAGCT CAGAGGCTAC CACACTGTTC CAGTTCAATG AGATGAAGAA ATACATAGGC 
TATGTGATCA GACAGCTGGA CCTGAGCCCA GACCCGAAGG CTTCCCAACA CTTTGCCAGG 
GTGGCTGTTG TGCAGCAGTC AACCTACGAG TCTGTGGACA ATGCCAGTGT GCCGCCTGTG 
AAGGTGGAAT TCTCACTGAC AGACTATGGT GCCAAGGAAA AGCTGTTGGA TTTCCTTAGC 
CGGAGGATGA CCCAACTTCA GGGAACCATG GGCCTGGGCA ATGCCATTGA ATACACTATA 
GAGAACATCT TTGAAAGTGC TCCAAACCCA CGGGACCTCA AAATTATGGT GCTGATGTTG 
ACTGGTGACA TGCAGAGGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT CCAGGCCAAG 
TGCAAGGGTT ACTTCTTTGT GGTTCTGGGC ATTGGCAGGA AGGTGAATAT CAAGGAGGTC 
TACAGCTTTG CCAGTGAGCC CAATGATGTC TTCTTCAAAT TTGTGGACAA ATCAACTGAG 
CTCAACGAGG AGCCTCTGAT GCGCTTTGGG AGGCTGCTCC CATCCTTTGT CAGCAGTGAA 
AATGCATTTT ACCTGCCTCC AGATCTCAGG AAACAGTGTG ATTGGTTCCA AGGGGACCAA 
CCAGTGAAGA ATGGCGTGAA GTTTGGTCAC AAACAGATAA ACCCTCCACA TACTGCTAAT 
TCAAGTCTTA CATCCAAAGT TGTGACCACA ATGAAGCCAG TGACAACGAC CAAACCAACA 
GCAATTGTAA ACCTGCCACC TGCTAAGCCA GCCCCAGCGA GGCCAGCCCC TGCTCAGCCA 
GTTCTTGCTA AGCCAGACCC CGCAAAACCA GCCCAAGCAA GGCCAGCCCC TGCTAAGCCA 
GCTTCTGCTA AGTTGGTGCC TCCTCAGCCA GTCCATGTTC AGCCAGCCCC TGCCCAGACA 
GCTTCTGTAA GACCAGCCCC CGCTAAGCCA GCCCCTCCTC AGCCAGCTGC TGCTAAGCCA 
GTCCCTGCTA AGCCAGCAGT CCCTGCTCAG CCAGCCCCTC CTCAGCCAGC TGCTGCTAAG 
CCAGTCCCTG CTAAGCCAGC AGTCCCTGCT CAGCCAGCCC CTCCTCAGCC AGCTGCTGCT 
AAGCCAGTCC CTGCTAAGCC AGCAGTCCCT GCTCAGCCAG CCGCTGCTCA ACCAATGCCT 
GCTCAGCCAG TCCTTACTAA ATCAGCCGCT GTGAAACCAG CCTCTGCCAA CAAGCCTGTG 
GCAGCCAAAC CTGTGGCCAC GAACACAGCC ACAGCCACAG CCAGACCTGC ATTAGCAGCG 
AAGCCTGCTG CAGCGAAGCC TGCAGCAACG AGACCCCTTG CTGCTGCCAT AAGGCCAGTT 
GCTACCAAGC CAGAGGCCCC CAGGCAACAG GCCAAACCAG CTGCCACCAA GCCAGCCACT 
ACTAAGCCCT TGGCGAGAGT GTCCCGAGAA GTCCAGGTCT CTGAAGTCAC GGAGAACAGT 
GCCAGACTCC ACTGGGAGCG GCCCGAGCCT TCCAGCTCCT TTTTTTATGA CCTCACTGTA 
ACCTCGGCCC ACGACCAGTC CTTGGTTCTG AGACAAAACC TCACTGTCAC TGACCGTGTC 
ATCGGAGGCC TACTGGCTGG ACAGCTGTAC CATGTCGTAG TGGTCTCCTA CCTGCAGTCT 
CAGGTCAGGG CCATCTACCA AGGAAGTTTC AATACGAAGA AAACCCAGCC TCCACCTCTT 
CAGGCAGCAC ACCGAGCATC CAGTTCAACC ATCAACCTCA TGGTGAACAC AGAACCATTG 
TTTCTCACTA AAACAGATAT ATGTAAGCTG TCCAGAGATG CTGGGACTTG TGTGGACTTC 
AAGTTACTAT GGCACTATGA CCTAGAGAGC AAAAGTTGCA AGAGATTCTG GTATGGAGGT 
TGTGGAGGCA ACGAGAACAG ATTCCACTCC CAGGAAGAAT GTGAAAAGAT GTGTAGTCCT 
GAGTTAACAG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101296.1
CDS	285..9539
Translation	MRKHRHLPLVAVFSLLLSGIATTHAQQHGDVKNGAAADIVFLVDSSWSAGKDRFLLVQEFLSDVVESLAVGDNDFHFALVRLNGNPHTEFLLNTYHSKQEVLSHIVNMSYIGGSNQTGKGLEYVIHSHLTEASGSRAADGVPQVIIVLTDGQSEDGFALPSAELKSADVNVFAVGVEGADERALGEVASEPLSMHVFNLENVTSLHGLVGNLVSCIHSSVNPERAGDKESLKDITAQDSADIIFLIDGSQNTGNANFDVIRDFLVNVLERLSVGNQQVQVGVVQYSEEPITMFSLNSYPSKAAVLDAVKGLSLVGGESANIGQALDFVVENHFTRAGGSRVEEGVPQVLVLISAGPSSDEIRDSVVALKQASVFSFGLGAQAASRAELQHIATDDSLVFTVPEFRSFGDLQEQILPYLVGVAQRHIVLQPPAIVTQVMEVNKRDIVFLVDGSSSLGPSNFNAIRDFVTRVIQRLEIGQDLVQVSVAQYADTVKPEFYLNSYTNKRDAITAVRKMRALNGSALYTGSSLDFVRNNLFTSSAGHRAAEGVPKLLVLITGGKSLDEVSQPAQELKRGSIMALAVGSKAADEDELKEIAFDSSLVFIPAEFRPAPLQNMLPSLMAPLRTLTGTTEESKRDILFLFDGSVNVLGQFPAVRDFLYRIIEELDVKPDGTRVAIAQFSDDVRLESRFSEHQTKAEILNLVKKMKLKTGKALNLGYALDYALRNIFVRSAGSRIEDNVQQFLVLLVAGRSSDAVAGPASSLKQRGVVPFIFQAKNANPSELEQIVLSPAFILAAESLPKIGDLQSQIVSLLKAEQGSGPVSGEKDVVFLIDGSEGVRSGFPLLKDFVQRVVESLDVGPDRVRVALVQYSDRTRPEFYLNSHMDQQGVISAIRRLTLLGGPTPNTGAALEFVLRNILTSSTGSRIAEGVPQLLIVLTAEPSGDDVRGPSVVLKQGGAVPIGIGIGNADISEMQTISFIPDFAVAIPTFRELGTIQQVISERVIQLNREELSSLKPILTPSTGAGVGSKKDVVFLIDGSRNAGPEFQYIRTLIERIVEYLDIGFDTTRVAVIQFSEDSKMEFPLNAHFSKDEVQNAVRRLRPKGGSQVYIGNALEYVLKNIFQRPLGSRIEEGVPQFLVLISSGKSDDEVDDSAVELKQFGVAPLTIARHTDQEELVKISLSPEYVYSVSTFRELPRLEQKLLTPITTLTSQQIHQILASTRYPPSVVESDAADIVFLIDSSDAVKPDGIAHIRDFVSRIVRRLNIGPSKVRIGVVQFSNDVFPEFYLKTHKSQSSVLEAIRRLRFKGGSPLNTGRALEFVARNLFVKSAGSRIEDGVPQHLVLFLGGKSQDDVARHAQVISSSGIVSLGIGDRNIDRTDLQTITNDPRLVFTVREFRELPNIEERVMLSFGPSGATPQPPGVDLPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFASEIVDTVYEDGDSIRVGLVQYNSDPTDEFFLRDFSTKRQIIDAINKVVYKGGRHANTRVGIEHLLRNHFVPEAGSRLDERVPQIAFVITGGKSVEDAQDVSLALTQKGVKVFAVGVRNIDSEEVGKIASNSATAFRVGSVQELSELSETVLETLHDAMHETLCPGVTDVSKACNLEVILGFDGSRDQNVFVSQKGLESKVDIILNRISQIQRISCSGNQLPTVRVSVMANTPSGPVEAFDFAEYQPELFEKFRNMRSQRPYVLTADTLKLYQNKFRQSSPDTVKVVIHFTDGADGDMADLYRASEELRQAGAQALILVGLERVANLERLMHLEFGRGFMYDRPLRLNLLDLDYELAEQLDNIAEKACCGVPCKCSGERGDRGPIGSIGPKGISGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEEGDKGLPGSSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGDSGRDSQQRGPKGETGDIGPMGLPGRDGIPGSPGDPGKDGGSGRRGPAGAKGNRGGPGQPGFEGEQGTRGSQGPPGPIGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGEPGPKGSIGNRGPRGETGDDGRDGVGSEGRRGKKGERGFPGYPGPKGTPGEPGADGPPGPKGIRGRRGNSGPPGATGQKGDPGYPGPSGHKGNRGDSVDQCALIQSIRDKCPCCYGPLECPVFPTELAFALDTSEGVTQDTFSRMREVLLGIVGDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSALLDSIQNLQVALTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRASPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPARRDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDLAFILDSSEATTLFQFNEMKKYIGYVIRQLDLSPDPKASQHFARVAVVQQSTYESVDNASVPPVKVEFSLTDYGAKEKLLDFLSRRMTQLQGTMGLGNAIEYTIENIFESAPNPRDLKIMVLMLTGDMQRQQLEEAQRAILQAKCKGYFFVVLGIGRKVNIKEVYSFASEPNDVFFKFVDKSTELNEEPLMRFGRLLPSFVSSENAFYLPPDLRKQCDWFQGDQPVKNGVKFGHKQINPPHTANSSLTSKVVTTMKPVTTTKPTAIVNLPPAKPAPARPAPAQPVLAKPDPAKPAQARPAPAKPASAKLVPPQPVHVQPAPAQTASVRPAPAKPAPPQPAAAKPVPAKPAVPAQPAPPQPAAAKPVPAKPAVPAQPAPPQPAAAKPVPAKPAVPAQPAAAQPMPAQPVLTKSAAVKPASANKPVAAKPVATNTATATARPALAAKPAAAKPAATRPLAAAIRPVATKPEAPRQQAKPAATKPATTKPLARVSREVQVSEVTENSARLHWERPEPSSSFFYDLTVTSAHDQSLVLRQNLTVTDRVIGGLLAGQLYHVVVVSYLQSQVRAIYQGSFNTKKTQPPPLQAAHRASSSTINLMVNTEPLFLTKTDICKLSRDAGTCVDFKLLWHYDLESKSCKRFWYGGCGGNENRFHSQEECEKMCSPELTV

Target ORF information:

RefSeq Version	XM_030245436.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245436.1

ORF Insert Sequence:

ATGAGGAAAC ACCGGCATCT GCCCCTAGTG GCTGTCTTTA GCCTTCTCCT CTCAGGCATT 
GCCACGACTC ATGCCCAACA GCATGGAGAT GTCAAAAACG GTGCGGCGGC TGACATAGTG 
TTTCTAGTGG ATTCCTCTTG GAGTGCTGGG AAGGACCGTT TCCTGCTTGT TCAAGAGTTT 
CTGTCTGATG TTGTAGAGTC TTTAGCTGTG GGAGACAATG ATTTTCACTT TGCTCTGGTC 
CGGCTCAACG GAAACCCACA TACCGAGTTC CTGTTAAATA CATACCACTC TAAACAAGAA 
GTCCTTTCTC ATATTGTGAA CATGTCTTAC ATTGGGGGAA GCAACCAAAC TGGAAAAGGA 
TTAGAATACG TAATTCACAG CCACCTCACT GAGGCTTCCG GAAGCCGGGC TGCTGACGGA 
GTCCCTCAGG TTATCATAGT ATTAACTGAT GGACAATCGG AGGATGGCTT CGCTCTGCCC 
TCAGCGGAAC TTAAGTCTGC GGACGTTAAT GTGTTTGCAG TCGGAGTTGA GGGTGCAGAT 
GAAAGAGCCC TAGGAGAAGT AGCAAGCGAA CCACTCAGTA TGCATGTTTT CAACCTAGAG 
AACGTTACCT CACTGCATGG CCTAGTAGGG AACTTAGTGT CCTGTATACA CTCCTCTGTG 
AATCCAGAAA GGGCTGGGGA CAAGGAAAGC CTTAAAGACA TCACAGCACA AGACTCAGCT 
GACATTATTT TCCTTATTGA TGGATCACAG AACACCGGAA ATGCCAATTT CGATGTCATT 
CGAGACTTCC TTGTAAATGT CCTTGAGAGA CTCTCAGTTG GAAACCAGCA GGTCCAAGTG 
GGGGTGGTAC AGTATAGCGA GGAGCCCATA ACCATGTTCT CCTTGAATAG CTACCCCTCC 
AAGGCTGCAG TTCTGGATGC CGTGAAAGGG CTCAGCTTGG TTGGGGGGGA GTCGGCCAAT 
ATAGGCCAGG CTCTTGACTT TGTAGTGGAG AATCACTTCA CTCGGGCAGG TGGCAGCCGC 
GTGGAGGAAG GGGTCCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG 
ATTCGAGATT CGGTGGTAGC TCTGAAGCAG GCTAGTGTGT TCTCGTTTGG CCTCGGAGCC 
CAGGCAGCCT CCAGGGCAGA GCTTCAGCAC ATAGCTACCG ATGACAGCTT GGTGTTTACT 
GTCCCGGAAT TCCGTAGCTT TGGGGATCTC CAGGAGCAAA TACTGCCGTA CCTTGTTGGT 
GTGGCCCAAA GGCACATTGT CTTGCAACCA CCAGCTATTG TCACACAAGT CATGGAAGTC 
AACAAGAGGG ACATAGTCTT CCTGGTGGAT GGTTCCTCCA GCCTGGGGCC ATCCAACTTC 
AATGCCATCC GAGACTTCGT TACCAGAGTC ATCCAGAGGT TGGAAATTGG ACAGGACCTG 
GTTCAGGTAT CAGTGGCTCA GTATGCAGAC ACTGTCAAGC CTGAGTTCTA TTTAAATTCC 
TATACCAACA AAAGGGATGC CATAACTGCT GTACGGAAGA TGAGGGCCCT GAATGGCTCA 
GCCCTGTACA CGGGATCTTC TCTGGACTTT GTTCGAAACA ACCTCTTCAC TAGCTCAGCC 
GGCCATAGGG CCGCTGAGGG GGTTCCTAAG CTCCTGGTGC TGATCACAGG CGGTAAGTCC 
CTCGATGAAG TCAGCCAGCC TGCCCAGGAG CTGAAGAGAG GCAGCATCAT GGCCTTGGCC 
GTTGGCAGCA AGGCCGCCGA TGAGGATGAG CTGAAAGAGA TCGCTTTCGA CTCCTCCCTG 
GTCTTCATTC CCGCTGAGTT CCGCCCTGCT CCCTTGCAAA ACATGCTGCC CAGCCTGATG 
GCACCTCTCA GGACTCTCAC TGGAACCACG GAAGAGAGCA AGCGGGACAT TCTTTTCCTC 
TTTGACGGCT CAGTCAATGT CTTGGGTCAG TTCCCTGCTG TCAGAGACTT TCTCTACAGG 
ATCATTGAGG AACTTGATGT GAAGCCGGAT GGGACCCGGG TAGCAATAGC TCAGTTCAGT 
GATGACGTCA GGCTGGAGTC CCGCTTCAGT GAGCACCAGA CGAAAGCTGA AATCCTTAAT 
CTCGTGAAGA AGATGAAGCT AAAAACAGGC AAAGCCCTCA ACCTGGGCTA CGCCCTGGAC 
TACGCGCTTA GGAACATCTT TGTGAGGTCG GCTGGCAGCC GCATCGAGGA TAACGTGCAG 
CAGTTCCTGG TGCTGCTGGT TGCTGGAAGG TCGTCAGATG CTGTGGCCGG GCCTGCAAGC 
TCCCTGAAGC AGAGAGGAGT GGTACCTTTC ATCTTTCAAG CCAAGAACGC CAACCCCAGT 
GAGCTGGAGC AGATTGTGCT GTCGCCTGCA TTCATCCTGG CTGCAGAGTC ACTCCCTAAG 
ATCGGAGACC TCCAATCACA GATTGTGAGC CTTCTGAAAG CAGAACAGGG TTCAGGCCCT 
GTATCAGGTG AAAAGGATGT GGTGTTTCTC ATTGATGGCT CTGAGGGTGT CCGCAGTGGC 
TTCCCCCTGC TAAAGGACTT TGTGCAGAGG GTTGTGGAGA GCTTGGATGT GGGTCCCGAC 
CGTGTGCGTG TGGCACTGGT GCAGTACAGT GACCGGACCA GGCCGGAGTT CTACCTGAAT 
TCCCACATGG ACCAGCAGGG TGTCATCAGC GCCATCCGCA GGCTGACACT GCTGGGCGGC 
CCAACCCCCA ACACAGGGGC GGCACTGGAG TTCGTGTTAA GGAACATTCT GACCAGTTCT 
ACTGGGAGCA GGATAGCAGA AGGTGTTCCT CAGCTCCTGA TTGTCCTCAC AGCAGAGCCA 
TCAGGGGATG ACGTGCGAGG CCCTTCAGTA GTCCTGAAGC AGGGCGGGGC TGTGCCCATT 
GGCATTGGCA TTGGGAATGC TGACATCTCT GAAATGCAGA CCATCTCCTT CATCCCTGAC 
TTTGCTGTGG CCATCCCCAC CTTCCGGGAG CTTGGGACGA TACAGCAGGT CATCTCTGAG 
AGGGTGATCC AGCTTAATCG TGAGGAGCTG AGCTCGTTGA AACCCATTTT GACGCCCTCA 
ACAGGTGCAG GTGTTGGCAG CAAGAAAGAT GTGGTCTTTC TCATCGATGG ATCCCGAAAT 
GCTGGCCCTG AGTTCCAGTA CATCCGTACC CTGATTGAGA GGATAGTCGA GTACCTGGAC 
ATAGGCTTTG ACACCACCCG GGTAGCCGTC ATCCAGTTCA GTGAGGACTC CAAGATGGAG 
TTTCCTCTGA ATGCCCACTT CAGCAAGGAT GAAGTACAGA ACGCTGTGCG CAGGCTGCGG 
CCCAAGGGTG GGAGTCAGGT CTACATCGGA AATGCCCTGG AGTACGTGCT GAAGAACATC 
TTCCAGAGGC CACTGGGGAG CCGGATAGAA GAGGGCGTTC CGCAGTTCCT AGTCCTCATT 
TCGTCTGGAA AGTCTGATGA TGAGGTGGAC GACTCAGCCG TGGAACTCAA GCAGTTCGGT 
GTGGCCCCTC TCACCATAGC CAGACACACA GACCAAGAGG AGCTGGTGAA GATCTCCCTA 
AGTCCTGAGT ACGTGTACTC CGTGAGCACC TTCAGGGAGC TGCCCAGACT GGAGCAGAAG 
CTGCTGACGC CCATCACCAC TCTAACCTCA CAGCAGATCC ATCAGATCCT GGCCAGTACC 
CGATATCCCC CTTCAGTGGT TGAAAGCGAT GCGGCTGACA TCGTCTTTCT GATTGACAGC 
TCTGATGCCG TCAAGCCCGA TGGCATCGCT CATATCCGAG ACTTTGTCAG CAGGATCGTT 
CGCAGACTCA ACATTGGTCC CAGTAAAGTG AGGATTGGGG TCGTGCAGTT TAGCAATGAT 
GTCTTCCCTG AGTTCTACCT GAAGACCCAC AAGTCCCAGA GCAGTGTGCT TGAAGCCATA 
CGGCGTCTGA GGTTCAAAGG AGGGTCTCCC CTAAACACTG GCAGAGCCCT GGAGTTTGTG 
GCAAGAAACC TCTTCGTGAA GTCTGCTGGG AGCCGGATAG AAGATGGGGT CCCTCAACAC 
CTGGTCTTAT TCCTGGGTGG GAAGTCTCAG GACGATGTGG CCAGGCATGC CCAAGTCATA 
AGCTCATCGG GGATTGTGAG CTTAGGAATA GGAGATCGGA ACATTGATAG GACGGACTTG 
CAAACCATCA CCAATGACCC CAGGCTGGTC TTCACAGTTC GGGAATTCAG AGAGCTGCCC 
AACATAGAAG AGAGGGTCAT GCTTTCCTTT GGACCCTCTG GGGCTACTCC TCAGCCTCCT 
GGGGTAGATT TACCTTCTCC TTCCCGGCCA GAGAAGAAGA AGGCCGACAT TGTGTTCCTG 
CTGGATGGTT CCATCAATTT TAGGAGGGAC AGTTTCCAGG AAGTGCTCCG TTTTGCATCT 
GAAATCGTGG ACACGGTCTA TGAAGACGGA GATTCCATCA GAGTGGGACT GGTCCAGTAC 
AACTCAGACC CCACGGATGA ATTCTTCCTG AGGGACTTTT CTACCAAAAG GCAGATTATC 
GATGCCATCA ACAAAGTCGT CTATAAAGGA GGGAGGCATG CCAACACCAG GGTGGGCATC 
GAGCACCTGC TGCGGAATCA CTTTGTGCCG GAGGCTGGTA GCCGCCTGGA TGAGCGGGTT 
CCTCAGATCG CCTTCGTGAT CACCGGAGGG AAGTCAGTGG AGGATGCTCA GGATGTGAGC 
CTGGCTCTCA CCCAGAAAGG TGTCAAGGTG TTTGCCGTTG GCGTGAGGAA CATCGACTCT 
GAGGAGGTGG GCAAGATAGC CTCCAACAGT GCCACAGCCT TCCGGGTGGG CAGCGTGCAG 
GAACTGTCCG AACTGAGTGA GACAGTGCTC GAGACTCTGC ATGATGCAAT GCATGAGACC 
CTCTGCCCTG GTGTGACCGA TGTCTCCAAA GCCTGCAATC TGGAAGTCAT TCTGGGGTTT 
GATGGATCTC GAGATCAGAA TGTGTTTGTG AGTCAGAAGG GCCTTGAGTC CAAGGTGGAC 
ATCATCTTGA ACAGAATCAG CCAGATACAA AGGATCAGCT GCAGTGGCAA CCAGCTGCCC 
ACTGTGAGGG TATCCGTGAT GGCGAACACG CCCTCTGGGC CAGTGGAGGC CTTTGACTTT 
GCCGAGTATC AGCCGGAGCT GTTTGAGAAG TTTCGCAACA TGCGCAGTCA GCGCCCATAC 
GTCCTCACAG CTGACACGCT GAAGTTGTAC CAGAACAAGT TCAGGCAGTC GTCGCCTGAT 
ACTGTGAAGG TAGTCATTCA CTTCACGGAT GGAGCAGATG GAGACATGGC TGACTTATAC 
AGAGCATCTG AGGAACTCCG GCAAGCAGGT GCCCAAGCCC TGATCCTGGT GGGTCTTGAA 
CGTGTGGCTA ACCTGGAGCG GCTGATGCAT CTGGAGTTCG GGCGGGGCTT CATGTATGAC 
AGGCCCCTGA GGCTCAACCT GCTTGACTTA GACTATGAAC TGGCAGAGCA GCTTGACAAT 
ATTGCTGAGA AAGCTTGCTG TGGGGTTCCA TGCAAGTGCT CTGGAGAGAG GGGAGACAGA 
GGGCCCATTG GCAGCATTGG GCCAAAGGGT ATCTCCGGGG AAGATGGCTA CCGAGGCTAC 
CCGGGAGACG AAGGCGGACC TGGTGAGCGA GGTCCACCTG GTGTGAATGG CACACAAGGT 
TTCCAGGGCT GTCCAGGCCA GAGGGGAGTG AAGGGTTCTC GAGGATTCCC AGGAGAGAAG 
GGTGAATTGG GAGAAATCGG CTTGGATGGT CTGGATGGTG AAGAGGGAGA CAAGGGGCTG 
CCTGGTTCTT CTGGAGAAAA GGGGAGTCCT GGAAGAAGGG GTGACAAAGG ACCCAAAGGA 
GACAAAGGCG AGAGAGGAGA TGTCGGGATT CGAGGTGACC CGGGTGACTC AGGACGGGAC 
AGCCAGCAGA GAGGACCCAA AGGAGAGACG GGAGACATCG GCCCCATGGG TCTCCCAGGC 
AGAGATGGCA TCCCTGGCAG CCCAGGAGAC CCTGGGAAGG ACGGTGGCTC TGGCCGAAGG 
GGCCCTGCAG GAGCTAAGGG CAACAGAGGT GGTCCTGGCC AGCCAGGCTT CGAAGGCGAG 
CAGGGTACCA GAGGCTCACA GGGCCCACCT GGTCCCATTG GTCCCCCAGG CCTGATTGGG 
GAACAAGGAA TTCCCGGACC TCGAGGAGGC GGAGGTACGG CTGGGGCTCC TGGAGAACGT 
GGCAGAACTG GTCCTCTGGG GAGGAAGGGT GAGCCAGGAG AGCCAGGGCC GAAGGGAAGC 
ATCGGGAACC GTGGACCCCG AGGAGAGACG GGAGATGACG GGAGAGATGG GGTTGGCAGT 
GAAGGACGCA GAGGCAAAAA AGGAGAAAGA GGCTTCCCTG GGTATCCGGG ACCAAAGGGC 
ACCCCTGGTG AGCCAGGGGC AGATGGACCA CCAGGACCCA AAGGCATCAG AGGTCGAAGG 
GGAAATTCAG GACCTCCAGG GGCAACTGGA CAAAAGGGAG ACCCTGGTTA CCCAGGACCA 
TCTGGTCACA AGGGCAACCG AGGCGACTCC GTTGATCAAT GCGCCCTCAT CCAGAGCATC 
AGAGACAAAT GCCCTTGCTG CTACGGGCCC CTGGAGTGCC CTGTATTCCC AACAGAACTC 
GCCTTTGCCC TGGATACCTC TGAGGGGGTG ACTCAGGACA CCTTCAGCCG GATGCGAGAG 
GTGCTTCTGG GCATTGTGGG CGATCTGACC ATTGCAGAGA GCAACTGTCC GCGGGGTGCC 
CGCGTGGCTG TGGTCACCTA TAACAATGAG GTGACTACAG AGATCCGGTT TGCTGACTCC 
AAGAAGAAGT CTGCCCTCTT AGACAGTATT CAGAACCTCC AAGTGGCCTT GACCTCTAAG 
CAGCAGAGCC TGGAGACCGC AATGTCATTC GTGGCCAGGA ATACATTCAA ACGTGTGAGG 
AGTGGCTTCC TGATGAGGAA AGTGGCTGTT TTCTTCAGCA ACAAGCCCAC CAGGGCATCC 
CCCCAGCTCC GAGAGGCCGT ACTCAAGCTT TCCGATGCGG GGATCACACC CTTGTTCCTT 
ACAAGCCAGG AGGACCGACA GCTCATCAAC GCTTTGCAGA TCAATAACAC AGCAGTGGGA 
CATGCCCTGG TTCTGCCTGC AAGGAGGGAT CTCACAGACT TCCTGAAGAA TGTCCTTACC 
TGCCATGTTT GCTTGGACAT TTGCAATATC GACCCATCCT GTGGCTTCGG CAGCTGGAGG 
CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATAGACCT GGCTTTCATC 
TTGGACAGCT CAGAGGCTAC CACACTGTTC CAGTTCAATG AGATGAAGAA ATACATAGGC 
TATGTGATCA GACAGCTGGA CCTGAGCCCA GACCCGAAGG CTTCCCAACA CTTTGCCAGG 
GTGGCTGTTG TGCAGCAGTC AACCTACGAG TCTGTGGACA ATGCCAGTGT GCCGCCTGTG 
AAGGTGGAAT TCTCACTGAC AGACTATGGT GCCAAGGAAA AGCTGTTGGA TTTCCTTAGC 
CGGAGGATGA CCCAACTTCA GGGAACCATG GGCCTGGGCA ATGCCATTGA ATACACTATA 
GAGAACATCT TTGAAAGTGC TCCAAACCCA CGGGACCTCA AAATTATGGT GCTGATGTTG 
ACTGGTGACA TGCAGAGGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT CCAGGCCAAG 
TGCAAGGGTT ACTTCTTTGT GGTTCTGGGC ATTGGCAGGA AGGTGAATAT CAAGGAGGTC 
TACAGCTTTG CCAGTGAGCC CAATGATGTC TTCTTCAAAT TTGTGGACAA ATCAACTGAG 
CTCAACGAGG AGCCTCTGAT GCGCTTTGGG AGGCTGCTCC CATCCTTTGT CAGCAGTGAA 
AATGCATTTT ACCTGCCTCC AGATCTCAGG AAACAGTGTG ATTGGTTCCA AGGGGACCAA 
CCAGTGAAGA ATGGCGTGAA GTTTGGTCAC AAACAGATAA ACCCTCCACA TACTGCTAAT 
TCAAGTCTTA CATCCAAAGT TGTGACCACA ATGAAGCCAG TGACAACGAC CAAACCAACA 
GCAATTGTAA ACCTGCCACC TGCTAAGCCA GCCCCAGCGA GGCCAGCCCC TGCTCAGCCA 
GTTCTTGCTA AGCCAGACCC CGCAAAACCA GCCCAAGCAA GGCCAGCCCC TGCTAAGCCA 
GCTTCTGCTA AGTTGGTGCC TCCTCAGCCA GTCCATGTTC AGCCAGCCCC TGCCCAGACA 
GCTTCTGTAA GACCAGCCCC CGCTAAGCCA GCCCCTCCTC AGCCAGCTGC TGCTAAGCCA 
GTCCCTGCTA AGCCAGCAGT CCCTGCTCAG CCAGCCCCTC CTCAGCCAGC TGCTGCTAAG 
CCAGTCCCTG CTAAGCCAGC AGTCCCTGCT CAGCCAGCCC CTCCTCAGCC AGCTGCTGCT 
AAGCCAGTCC CTGCTAAGCC AGCAGTCCCT GCTCAGCCAG CCGCTGCTCA ACCAATGCCT 
GCTCAGCCAG TCCTTACTAA ATCAGCCGCT GTGAAACCAG CCTCTGCCAA CAAGCCTGTG 
GCAGCCAAAC CTGTGGCCAC GAACACAGCC ACAGCCACAG CCAGACCTGC ATTAGCAGCG 
AAGCCTGCTG CAGCGAAGCC TGCAGCAACG AGACCCCTTG CTGCTGCCAT AAGGCCAGTT 
GCTACCAAGC CAGAGGCCCC CAGGCAACAG GCCAAACCAG CTGCCACCAA GCCAGCCACT 
ACTAAGCCCT TGGCGAGAGT GTCCCGAGAA GTCCAGGTCT CTGAAGTCAC GGAGAACAGT 
GCCAGACTCC ACTGGGAGCG GCCCGAGCCT TCCAGCTCCT TTTTTTATGA CCTCACTGTA 
ACCTCGGCCC ACGACCAGTC CTTGGTTCTG AGACAAAACC TCACTGTCAC TGACCGTGTC 
ATCGGAGGCC TACTGGCTGG ACAGCTGTAC CATGTCGTAG TGGTCTCCTA CCTGCAGTCT 
CAGGTCAGGG CCATCTACCA AGGAAGTTTC AATACGAAGA AAACCCAGCC TCCACCTCTT 
CAGGCAGCAC ACCGAGCATC CAGTTCAACC ATCAACCTCA TGGTGAACAC AGAACCATTG 
TTTCTCACTA AAACAGATAT ATGTAAGCTG TCCAGAGATG CTGGGACTTG TGTGGACTTC 
AAGTTACTAT GGCACTATGA CCTAGAGAGC AAAAGTTGCA AGAGATTCTG GTATGGAGGT 
TGTGGAGGCA ACGAGAACAG ATTCCACTCC CAGGAAGAAT GTGAAAAGAT GTGTAGTCCT 
GAGTTAACAG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a3 ( XM_030245437.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101297.1 Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant X2, mRNA.

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CloneID	OMu84997
Clone ID Related Accession (Same CDS sequence)	XM_030245437.1
Accession Version	XM_030245437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-3(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAAC ACCGGCATCT GCCCCTAGTG GCTGTCTTTA GCCTTCTCCT CTCAGGCATT 
GCCACGACTC ATGCCCAACA GCATGGAGCA CAAGACTCAG CTGACATTAT TTTCCTTATT 
GATGGATCAC AGAACACCGG AAATGCCAAT TTCGATGTCA TTCGAGACTT CCTTGTAAAT 
GTCCTTGAGA GACTCTCAGT TGGAAACCAG CAGGTCCAAG TGGGGGTGGT ACAGTATAGC 
GAGGAGCCCA TAACCATGTT CTCCTTGAAT AGCTACCCCT CCAAGGCTGC AGTTCTGGAT 
GCCGTGAAAG GGCTCAGCTT GGTTGGGGGG GAGTCGGCCA ATATAGGCCA GGCTCTTGAC 
TTTGTAGTGG AGAATCACTT CACTCGGGCA GGTGGCAGCC GCGTGGAGGA AGGGGTCCCC 
CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATTCGAGA TTCGGTGGTA 
GCTCTGAAGC AGGCTAGTGT GTTCTCGTTT GGCCTCGGAG CCCAGGCAGC CTCCAGGGCA 
GAGCTTCAGC ACATAGCTAC CGATGACAGC TTGGTGTTTA CTGTCCCGGA ATTCCGTAGC 
TTTGGGGATC TCCAGGAGCA AATACTGCCG TACCTTGTTG GTGTGGCCCA AAGGCACATT 
GTCTTGCAAC CACCAGCTAT TGTCACACAA GTCATGGAAG TCAACAAGAG GGACATAGTC 
TTCCTGGTGG ATGGTTCCTC CAGCCTGGGG CCATCCAACT TCAATGCCAT CCGAGACTTC 
GTTACCAGAG TCATCCAGAG GTTGGAAATT GGACAGGACC TGGTTCAGGT ATCAGTGGCT 
CAGTATGCAG ACACTGTCAA GCCTGAGTTC TATTTAAATT CCTATACCAA CAAAAGGGAT 
GCCATAACTG CTGTACGGAA GATGAGGGCC CTGAATGGCT CAGCCCTGTA CACGGGATCT 
TCTCTGGACT TTGTTCGAAA CAACCTCTTC ACTAGCTCAG CCGGCCATAG GGCCGCTGAG 
GGGGTTCCTA AGCTCCTGGT GCTGATCACA GGCGGTAAGT CCCTCGATGA AGTCAGCCAG 
CCTGCCCAGG AGCTGAAGAG AGGCAGCATC ATGGCCTTGG CCGTTGGCAG CAAGGCCGCC 
GATGAGGATG AGCTGAAAGA GATCGCTTTC GACTCCTCCC TGGTCTTCAT TCCCGCTGAG 
TTCCGCCCTG CTCCCTTGCA AAACATGCTG CCCAGCCTGA TGGCACCTCT CAGGACTCTC 
ACTGGAACCA CGGAAGTTCA CGTAAACAAA CGGGATATCA TCTTCCTTTT GGATGGCTCC 
GACAACGTTG GGAAAAACAA TTTCCCTTAT GTGCGTGACT TTGTAACCAA CCTAGTTAAC 
AGCCTCGATG TTGGAAGTGA CAATATCCGT GTTGGCTTGG TGCAGTTTAG TGACACTCCG 
GTCACGGAGT TCTCTCTAGA CACTTACCAG ACCAAGTCCG AGCTGCTCGC TCACCTGAGG 
CGCTTGCAGC TCAAGGGGGG CTCCGGCTTG AACGCAGGCT CTGCCTTGAG CTATATCCAT 
GCCAATCACT TCACGGAGGC AGGAGGCAGC AGGACTCGTG AACACGTGCC TCAGCTGCTG 
CTCCTGCTCA TGGCCGGGCC CTCTGAGGAT GCCTATTTGC AAGCTGCCAA TGCCCTGGTG 
CGCTCAGGCG TGCTGACCTT CTGTGTGGGG ACCAATCGGG CGGATAAGGC TGAGTTAGAG 
CATATTGCTT TTAATCCCAG CCTGGTATAC CTCATGGACG ATTTCCGCTC CCTGCCATCT 
TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACATATGTTA GTGGAGGTGT ACAGGAAGTT 
CCACTATCCC AGCCAGAGAG CAAGCGGGAC ATTCTTTTCC TCTTTGACGG CTCAGTCAAT 
GTCTTGGGTC AGTTCCCTGC TGTCAGAGAC TTTCTCTACA GGATCATTGA GGAACTTGAT 
GTGAAGCCGG ATGGGACCCG GGTAGCAATA GCTCAGTTCA GTGATGACGT CAGGCTGGAG 
TCCCGCTTCA GTGAGCACCA GACGAAAGCT GAAATCCTTA ATCTCGTGAA GAAGATGAAG 
CTAAAAACAG GCAAAGCCCT CAACCTGGGC TACGCCCTGG ACTACGCGCT TAGGAACATC 
TTTGTGAGGT CGGCTGGCAG CCGCATCGAG GATAACGTGC AGCAGTTCCT GGTGCTGCTG 
GTTGCTGGAA GGTCGTCAGA TGCTGTGGCC GGGCCTGCAA GCTCCCTGAA GCAGAGAGGA 
GTGGTACCTT TCATCTTTCA AGCCAAGAAC GCCAACCCCA GTGAGCTGGA GCAGATTGTG 
CTGTCGCCTG CATTCATCCT GGCTGCAGAG TCACTCCCTA AGATCGGAGA CCTCCAATCA 
CAGATTGTGA GCCTTCTGAA AGCAGAACAG GGTTCAGGCC CTGTATCAGG TGAAAAGGAT 
GTGGTGTTTC TCATTGATGG CTCTGAGGGT GTCCGCAGTG GCTTCCCCCT GCTAAAGGAC 
TTTGTGCAGA GGGTTGTGGA GAGCTTGGAT GTGGGTCCCG ACCGTGTGCG TGTGGCACTG 
GTGCAGTACA GTGACCGGAC CAGGCCGGAG TTCTACCTGA ATTCCCACAT GGACCAGCAG 
GGTGTCATCA GCGCCATCCG CAGGCTGACA CTGCTGGGCG GCCCAACCCC CAACACAGGG 
GCGGCACTGG AGTTCGTGTT AAGGAACATT CTGACCAGTT CTACTGGGAG CAGGATAGCA 
GAAGGTGTTC CTCAGCTCCT GATTGTCCTC ACAGCAGAGC CATCAGGGGA TGACGTGCGA 
GGCCCTTCAG TAGTCCTGAA GCAGGGCGGG GCTGTGCCCA TTGGCATTGG CATTGGGAAT 
GCTGACATCT CTGAAATGCA GACCATCTCC TTCATCCCTG ACTTTGCTGT GGCCATCCCC 
ACCTTCCGGG AGCTTGGGAC GATACAGCAG GTCATCTCTG AGAGGGTGAT CCAGCTTAAT 
CGTGAGGAGC TGAGCTCGTT GAAACCCATT TTGACGCCCT CAACAGGTGC AGGTGTTGGC 
AGCAAGAAAG ATGTGGTCTT TCTCATCGAT GGATCCCGAA ATGCTGGCCC TGAGTTCCAG 
TACATCCGTA CCCTGATTGA GAGGATAGTC GAGTACCTGG ACATAGGCTT TGACACCACC 
CGGGTAGCCG TCATCCAGTT CAGTGAGGAC TCCAAGATGG AGTTTCCTCT GAATGCCCAC 
TTCAGCAAGG ATGAAGTACA GAACGCTGTG CGCAGGCTGC GGCCCAAGGG TGGGAGTCAG 
GTCTACATCG GAAATGCCCT GGAGTACGTG CTGAAGAACA TCTTCCAGAG GCCACTGGGG 
AGCCGGATAG AAGAGGGCGT TCCGCAGTTC CTAGTCCTCA TTTCGTCTGG AAAGTCTGAT 
GATGAGGTGG ACGACTCAGC CGTGGAACTC AAGCAGTTCG GTGTGGCCCC TCTCACCATA 
GCCAGACACA CAGACCAAGA GGAGCTGGTG AAGATCTCCC TAAGTCCTGA GTACGTGTAC 
TCCGTGAGCA CCTTCAGGGA GCTGCCCAGA CTGGAGCAGA AGCTGCTGAC GCCCATCACC 
ACTCTAACCT CACAGCAGAT CCATCAGATC CTGGCCAGTA CCCGATATCC CCCTTCAGTG 
GTTGAAAGCG ATGCGGCTGA CATCGTCTTT CTGATTGACA GCTCTGATGC CGTCAAGCCC 
GATGGCATCG CTCATATCCG AGACTTTGTC AGCAGGATCG TTCGCAGACT CAACATTGGT 
CCCAGTAAAG TGAGGATTGG GGTCGTGCAG TTTAGCAATG ATGTCTTCCC TGAGTTCTAC 
CTGAAGACCC ACAAGTCCCA GAGCAGTGTG CTTGAAGCCA TACGGCGTCT GAGGTTCAAA 
GGAGGGTCTC CCCTAAACAC TGGCAGAGCC CTGGAGTTTG TGGCAAGAAA CCTCTTCGTG 
AAGTCTGCTG GGAGCCGGAT AGAAGATGGG GTCCCTCAAC ACCTGGTCTT ATTCCTGGGT 
GGGAAGTCTC AGGACGATGT GGCCAGGCAT GCCCAAGTCA TAAGCTCATC GGGGATTGTG 
AGCTTAGGAA TAGGAGATCG GAACATTGAT AGGACGGACT TGCAAACCAT CACCAATGAC 
CCCAGGCTGG TCTTCACAGT TCGGGAATTC AGAGAGCTGC CCAACATAGA AGAGAGGGTC 
ATGCTTTCCT TTGGACCCTC TGGGGCTACT CCTCAGCCTC CTGGGGTAGA TTTACCTTCT 
CCTTCCCGGC CAGAGAAGAA GAAGGCCGAC ATTGTGTTCC TGCTGGATGG TTCCATCAAT 
TTTAGGAGGG ACAGTTTCCA GGAAGTGCTC CGTTTTGCAT CTGAAATCGT GGACACGGTC 
TATGAAGACG GAGATTCCAT CAGAGTGGGA CTGGTCCAGT ACAACTCAGA CCCCACGGAT 
GAATTCTTCC TGAGGGACTT TTCTACCAAA AGGCAGATTA TCGATGCCAT CAACAAAGTC 
GTCTATAAAG GAGGGAGGCA TGCCAACACC AGGGTGGGCA TCGAGCACCT GCTGCGGAAT 
CACTTTGTGC CGGAGGCTGG TAGCCGCCTG GATGAGCGGG TTCCTCAGAT CGCCTTCGTG 
ATCACCGGAG GGAAGTCAGT GGAGGATGCT CAGGATGTGA GCCTGGCTCT CACCCAGAAA 
GGTGTCAAGG TGTTTGCCGT TGGCGTGAGG AACATCGACT CTGAGGAGGT GGGCAAGATA 
GCCTCCAACA GTGCCACAGC CTTCCGGGTG GGCAGCGTGC AGGAACTGTC CGAACTGAGT 
GAGACAGTGC TCGAGACTCT GCATGATGCA ATGCATGAGA CCCTCTGCCC TGGTGTGACC 
GATGTCTCCA AAGCCTGCAA TCTGGAAGTC ATTCTGGGGT TTGATGGATC TCGAGATCAG 
AATGTGTTTG TGAGTCAGAA GGGCCTTGAG TCCAAGGTGG ACATCATCTT GAACAGAATC 
AGCCAGATAC AAAGGATCAG CTGCAGTGGC AACCAGCTGC CCACTGTGAG GGTATCCGTG 
ATGGCGAACA CGCCCTCTGG GCCAGTGGAG GCCTTTGACT TTGCCGAGTA TCAGCCGGAG 
CTGTTTGAGA AGTTTCGCAA CATGCGCAGT CAGCGCCCAT ACGTCCTCAC AGCTGACACG 
CTGAAGTTGT ACCAGAACAA GTTCAGGCAG TCGTCGCCTG ATACTGTGAA GGTAGTCATT 
CACTTCACGG ATGGAGCAGA TGGAGACATG GCTGACTTAT ACAGAGCATC TGAGGAACTC 
CGGCAAGCAG GTGCCCAAGC CCTGATCCTG GTGGGTCTTG AACGTGTGGC TAACCTGGAG 
CGGCTGATGC ATCTGGAGTT CGGGCGGGGC TTCATGTATG ACAGGCCCCT GAGGCTCAAC 
CTGCTTGACT TAGACTATGA ACTGGCAGAG CAGCTTGACA ATATTGCTGA GAAAGCTTGC 
TGTGGGGTTC CATGCAAGTG CTCTGGAGAG AGGGGAGACA GAGGGCCCAT TGGCAGCATT 
GGGCCAAAGG GTATCTCCGG GGAAGATGGC TACCGAGGCT ACCCGGGAGA CGAAGGCGGA 
CCTGGTGAGC GAGGTCCACC TGGTGTGAAT GGCACACAAG GTTTCCAGGG CTGTCCAGGC 
CAGAGGGGAG TGAAGGGTTC TCGAGGATTC CCAGGAGAGA AGGGTGAATT GGGAGAAATC 
GGCTTGGATG GTCTGGATGG TGAAGAGGGA GACAAGGGGC TGCCTGGTTC TTCTGGAGAA 
AAGGGGAGTC CTGGAAGAAG GGGTGACAAA GGACCCAAAG GAGACAAAGG CGAGAGAGGA 
GATGTCGGGA TTCGAGGTGA CCCGGGTGAC TCAGGACGGG ACAGCCAGCA GAGAGGACCC 
AAAGGAGAGA CGGGAGACAT CGGCCCCATG GGTCTCCCAG GCAGAGATGG CATCCCTGGC 
AGCCCAGGAG ACCCTGGGAA GGACGGTGGC TCTGGCCGAA GGGGCCCTGC AGGAGCTAAG 
GGCAACAGAG GTGGTCCTGG CCAGCCAGGC TTCGAAGGCG AGCAGGGTAC CAGAGGCTCA 
CAGGGCCCAC CTGGTCCCAT TGGTCCCCCA GGCCTGATTG GGGAACAAGG AATTCCCGGA 
CCTCGAGGAG GCGGAGGTAC GGCTGGGGCT CCTGGAGAAC GTGGCAGAAC TGGTCCTCTG 
GGGAGGAAGG GTGAGCCAGG AGAGCCAGGG CCGAAGGGAA GCATCGGGAA CCGTGGACCC 
CGAGGAGAGA CGGGAGATGA CGGGAGAGAT GGGGTTGGCA GTGAAGGACG CAGAGGCAAA 
AAAGGAGAAA GAGGCTTCCC TGGGTATCCG GGACCAAAGG GCACCCCTGG TGAGCCAGGG 
GCAGATGGAC CACCAGGACC CAAAGGCATC AGAGGTCGAA GGGGAAATTC AGGACCTCCA 
GGGGCAACTG GACAAAAGGG AGACCCTGGT TACCCAGGAC CATCTGGTCA CAAGGGCAAC 
CGAGGCGACT CCGTTGATCA ATGCGCCCTC ATCCAGAGCA TCAGAGACAA ATGCCCTTGC 
TGCTACGGGC CCCTGGAGTG CCCTGTATTC CCAACAGAAC TCGCCTTTGC CCTGGATACC 
TCTGAGGGGG TGACTCAGGA CACCTTCAGC CGGATGCGAG AGGTGCTTCT GGGCATTGTG 
GGCGATCTGA CCATTGCAGA GAGCAACTGT CCGCGGGGTG CCCGCGTGGC TGTGGTCACC 
TATAACAATG AGGTGACTAC AGAGATCCGG TTTGCTGACT CCAAGAAGAA GTCTGCCCTC 
TTAGACAGTA TTCAGAACCT CCAAGTGGCC TTGACCTCTA AGCAGCAGAG CCTGGAGACC 
GCAATGTCAT TCGTGGCCAG GAATACATTC AAACGTGTGA GGAGTGGCTT CCTGATGAGG 
AAAGTGGCTG TTTTCTTCAG CAACAAGCCC ACCAGGGCAT CCCCCCAGCT CCGAGAGGCC 
GTACTCAAGC TTTCCGATGC GGGGATCACA CCCTTGTTCC TTACAAGCCA GGAGGACCGA 
CAGCTCATCA ACGCTTTGCA GATCAATAAC ACAGCAGTGG GACATGCCCT GGTTCTGCCT 
GCAAGGAGGG ATCTCACAGA CTTCCTGAAG AATGTCCTTA CCTGCCATGT TTGCTTGGAC 
ATTTGCAATA TCGACCCATC CTGTGGCTTC GGCAGCTGGA GGCCTTCCTT CAGGGACCGG 
AGGGCAGCAG GCAGTGATGT GGACATAGAC CTGGCTTTCA TCTTGGACAG CTCAGAGGCT 
ACCACACTGT TCCAGTTCAA TGAGATGAAG AAATACATAG GCTATGTGAT CAGACAGCTG 
GACCTGAGCC CAGACCCGAA GGCTTCCCAA CACTTTGCCA GGGTGGCTGT TGTGCAGCAG 
TCAACCTACG AGTCTGTGGA CAATGCCAGT GTGCCGCCTG TGAAGGTGGA ATTCTCACTG 
ACAGACTATG GTGCCAAGGA AAAGCTGTTG GATTTCCTTA GCCGGAGGAT GACCCAACTT 
CAGGGAACCA TGGGCCTGGG CAATGCCATT GAATACACTA TAGAGAACAT CTTTGAAAGT 
GCTCCAAACC CACGGGACCT CAAAATTATG GTGCTGATGT TGACTGGTGA CATGCAGAGG 
CAGCAGCTGG AGGAGGCCCA GAGAGCCATC CTCCAGGCCA AGTGCAAGGG TTACTTCTTT 
GTGGTTCTGG GCATTGGCAG GAAGGTGAAT ATCAAGGAGG TCTACAGCTT TGCCAGTGAG 
CCCAATGATG TCTTCTTCAA ATTTGTGGAC AAATCAACTG AGCTCAACGA GGAGCCTCTG 
ATGCGCTTTG GGAGGCTGCT CCCATCCTTT GTCAGCAGTG AAAATGCATT TTACCTGCCT 
CCAGATCTCA GGAAACAGTG TGATTGGTTC CAAGGGGACC AACCAGTGAA GAATGGCGTG 
AAGTTTGGTC ACAAACAGAT AAACCCTCCA CATACTGCTA ATTCAAGTCT TACATCCAAA 
GTTGTGACCA CAATGAAGCC AGTGACAACG ACCAAACCAA CAGCAATTGT AAACCTGCCA 
CCTGCTAAGC CAGCCCCAGC GAGGCCAGCC CCTGCTCAGC CAGTTCTTGC TAAGCCAGAC 
CCCGCAAAAC CAGCCCAAGC AAGGCCAGCC CCTGCTAAGC CAGCTTCTGC TAAGTTGGTG 
CCTCCTCAGC CAGTCCATGT TCAGCCAGCC CCTGCCCAGA CAGCTTCTGT AAGACCAGCC 
CCCGCTAAGC CAGCCCCTCC TCAGCCAGCT GCTGCTAAGC CAGTCCCTGC TAAGCCAGCA 
GTCCCTGCTC AGCCAGCCCC TCCTCAGCCA GCTGCTGCTA AGCCAGTCCC TGCTAAGCCA 
GCAGTCCCTG CTCAGCCAGC CCCTCCTCAG CCAGCTGCTG CTAAGCCAGT CCCTGCTAAG 
CCAGCAGTCC CTGCTCAGCC AGCCGCTGCT CAACCAATGC CTGCTCAGCC AGTCCTTACT 
AAATCAGCCG CTGTGAAACC AGCCTCTGCC AACAAGCCTG TGGCAGCCAA ACCTGTGGCC 
ACGAACACAG CCACAGCCAC AGCCAGACCT GCATTAGCAG CGAAGCCTGC TGCAGCGAAG 
CCTGCAGCAA CGAGACCCCT TGCTGCTGCC ATAAGGCCAG TTGCTACCAA GCCAGAGGCC 
CCCAGGCAAC AGGCCAAACC AGCTGCCACC AAGCCAGCCA CTACTAAGCC CTTGGCGAGA 
GTGTCCCGAG AAGTCCAGGT CTCTGAAGTC ACGGAGAACA GTGCCAGACT CCACTGGGAG 
CGGCCCGAGC CTTCCAGCTC CTTTTTTTAT GACCTCACTG TAACCTCGGC CCACGACCAG 
TCCTTGGTTC TGAGACAAAA CCTCACTGTC ACTGACCGTG TCATCGGAGG CCTACTGGCT 
GGACAGCTGT ACCATGTCGT AGTGGTCTCC TACCTGCAGT CTCAGGTCAG GGCCATCTAC 
CAAGGAAGTT TCAATACGAA GAAAACCCAG CCTCCACCTC TTCAGGCAGC ACACCGAGCA 
TCCAGTTCAA CCATCAACCT CATGGTGAAC ACAGAACCAT TGTTTCTCAC TAAAACAGAT 
ATATGTAAGC TGTCCAGAGA TGCTGGGACT TGTGTGGACT TCAAGTTACT ATGGCACTAT 
GACCTAGAGA GCAAAAGTTG CAAGAGATTC TGGTATGGAG GTTGTGGAGG CAACGAGAAC 
AGATTCCACT CCCAGGAAGA ATGTGAAAAG ATGTGTAGTC CTGAGTTAAC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101297.1
CDS	284..9520
Translation	MRKHRHLPLVAVFSLLLSGIATTHAQQHGAQDSADIIFLIDGSQNTGNANFDVIRDFLVNVLERLSVGNQQVQVGVVQYSEEPITMFSLNSYPSKAAVLDAVKGLSLVGGESANIGQALDFVVENHFTRAGGSRVEEGVPQVLVLISAGPSSDEIRDSVVALKQASVFSFGLGAQAASRAELQHIATDDSLVFTVPEFRSFGDLQEQILPYLVGVAQRHIVLQPPAIVTQVMEVNKRDIVFLVDGSSSLGPSNFNAIRDFVTRVIQRLEIGQDLVQVSVAQYADTVKPEFYLNSYTNKRDAITAVRKMRALNGSALYTGSSLDFVRNNLFTSSAGHRAAEGVPKLLVLITGGKSLDEVSQPAQELKRGSIMALAVGSKAADEDELKEIAFDSSLVFIPAEFRPAPLQNMLPSLMAPLRTLTGTTEVHVNKRDIIFLLDGSDNVGKNNFPYVRDFVTNLVNSLDVGSDNIRVGLVQFSDTPVTEFSLDTYQTKSELLAHLRRLQLKGGSGLNAGSALSYIHANHFTEAGGSRTREHVPQLLLLLMAGPSEDAYLQAANALVRSGVLTFCVGTNRADKAELEHIAFNPSLVYLMDDFRSLPSLPQQLIQPLTTYVSGGVQEVPLSQPESKRDILFLFDGSVNVLGQFPAVRDFLYRIIEELDVKPDGTRVAIAQFSDDVRLESRFSEHQTKAEILNLVKKMKLKTGKALNLGYALDYALRNIFVRSAGSRIEDNVQQFLVLLVAGRSSDAVAGPASSLKQRGVVPFIFQAKNANPSELEQIVLSPAFILAAESLPKIGDLQSQIVSLLKAEQGSGPVSGEKDVVFLIDGSEGVRSGFPLLKDFVQRVVESLDVGPDRVRVALVQYSDRTRPEFYLNSHMDQQGVISAIRRLTLLGGPTPNTGAALEFVLRNILTSSTGSRIAEGVPQLLIVLTAEPSGDDVRGPSVVLKQGGAVPIGIGIGNADISEMQTISFIPDFAVAIPTFRELGTIQQVISERVIQLNREELSSLKPILTPSTGAGVGSKKDVVFLIDGSRNAGPEFQYIRTLIERIVEYLDIGFDTTRVAVIQFSEDSKMEFPLNAHFSKDEVQNAVRRLRPKGGSQVYIGNALEYVLKNIFQRPLGSRIEEGVPQFLVLISSGKSDDEVDDSAVELKQFGVAPLTIARHTDQEELVKISLSPEYVYSVSTFRELPRLEQKLLTPITTLTSQQIHQILASTRYPPSVVESDAADIVFLIDSSDAVKPDGIAHIRDFVSRIVRRLNIGPSKVRIGVVQFSNDVFPEFYLKTHKSQSSVLEAIRRLRFKGGSPLNTGRALEFVARNLFVKSAGSRIEDGVPQHLVLFLGGKSQDDVARHAQVISSSGIVSLGIGDRNIDRTDLQTITNDPRLVFTVREFRELPNIEERVMLSFGPSGATPQPPGVDLPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFASEIVDTVYEDGDSIRVGLVQYNSDPTDEFFLRDFSTKRQIIDAINKVVYKGGRHANTRVGIEHLLRNHFVPEAGSRLDERVPQIAFVITGGKSVEDAQDVSLALTQKGVKVFAVGVRNIDSEEVGKIASNSATAFRVGSVQELSELSETVLETLHDAMHETLCPGVTDVSKACNLEVILGFDGSRDQNVFVSQKGLESKVDIILNRISQIQRISCSGNQLPTVRVSVMANTPSGPVEAFDFAEYQPELFEKFRNMRSQRPYVLTADTLKLYQNKFRQSSPDTVKVVIHFTDGADGDMADLYRASEELRQAGAQALILVGLERVANLERLMHLEFGRGFMYDRPLRLNLLDLDYELAEQLDNIAEKACCGVPCKCSGERGDRGPIGSIGPKGISGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEEGDKGLPGSSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGDSGRDSQQRGPKGETGDIGPMGLPGRDGIPGSPGDPGKDGGSGRRGPAGAKGNRGGPGQPGFEGEQGTRGSQGPPGPIGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGEPGPKGSIGNRGPRGETGDDGRDGVGSEGRRGKKGERGFPGYPGPKGTPGEPGADGPPGPKGIRGRRGNSGPPGATGQKGDPGYPGPSGHKGNRGDSVDQCALIQSIRDKCPCCYGPLECPVFPTELAFALDTSEGVTQDTFSRMREVLLGIVGDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSALLDSIQNLQVALTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRASPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPARRDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDLAFILDSSEATTLFQFNEMKKYIGYVIRQLDLSPDPKASQHFARVAVVQQSTYESVDNASVPPVKVEFSLTDYGAKEKLLDFLSRRMTQLQGTMGLGNAIEYTIENIFESAPNPRDLKIMVLMLTGDMQRQQLEEAQRAILQAKCKGYFFVVLGIGRKVNIKEVYSFASEPNDVFFKFVDKSTELNEEPLMRFGRLLPSFVSSENAFYLPPDLRKQCDWFQGDQPVKNGVKFGHKQINPPHTANSSLTSKVVTTMKPVTTTKPTAIVNLPPAKPAPARPAPAQPVLAKPDPAKPAQARPAPAKPASAKLVPPQPVHVQPAPAQTASVRPAPAKPAPPQPAAAKPVPAKPAVPAQPAPPQPAAAKPVPAKPAVPAQPAPPQPAAAKPVPAKPAVPAQPAAAQPMPAQPVLTKSAAVKPASANKPVAAKPVATNTATATARPALAAKPAAAKPAATRPLAAAIRPVATKPEAPRQQAKPAATKPATTKPLARVSREVQVSEVTENSARLHWERPEPSSSFFYDLTVTSAHDQSLVLRQNLTVTDRVIGGLLAGQLYHVVVVSYLQSQVRAIYQGSFNTKKTQPPPLQAAHRASSSTINLMVNTEPLFLTKTDICKLSRDAGTCVDFKLLWHYDLESKSCKRFWYGGCGGNENRFHSQEECEKMCSPELTV

Target ORF information:

RefSeq Version	XM_030245437.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245437.1

ORF Insert Sequence:

ATGAGGAAAC ACCGGCATCT GCCCCTAGTG GCTGTCTTTA GCCTTCTCCT CTCAGGCATT 
GCCACGACTC ATGCCCAACA GCATGGAGCA CAAGACTCAG CTGACATTAT TTTCCTTATT 
GATGGATCAC AGAACACCGG AAATGCCAAT TTCGATGTCA TTCGAGACTT CCTTGTAAAT 
GTCCTTGAGA GACTCTCAGT TGGAAACCAG CAGGTCCAAG TGGGGGTGGT ACAGTATAGC 
GAGGAGCCCA TAACCATGTT CTCCTTGAAT AGCTACCCCT CCAAGGCTGC AGTTCTGGAT 
GCCGTGAAAG GGCTCAGCTT GGTTGGGGGG GAGTCGGCCA ATATAGGCCA GGCTCTTGAC 
TTTGTAGTGG AGAATCACTT CACTCGGGCA GGTGGCAGCC GCGTGGAGGA AGGGGTCCCC 
CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATTCGAGA TTCGGTGGTA 
GCTCTGAAGC AGGCTAGTGT GTTCTCGTTT GGCCTCGGAG CCCAGGCAGC CTCCAGGGCA 
GAGCTTCAGC ACATAGCTAC CGATGACAGC TTGGTGTTTA CTGTCCCGGA ATTCCGTAGC 
TTTGGGGATC TCCAGGAGCA AATACTGCCG TACCTTGTTG GTGTGGCCCA AAGGCACATT 
GTCTTGCAAC CACCAGCTAT TGTCACACAA GTCATGGAAG TCAACAAGAG GGACATAGTC 
TTCCTGGTGG ATGGTTCCTC CAGCCTGGGG CCATCCAACT TCAATGCCAT CCGAGACTTC 
GTTACCAGAG TCATCCAGAG GTTGGAAATT GGACAGGACC TGGTTCAGGT ATCAGTGGCT 
CAGTATGCAG ACACTGTCAA GCCTGAGTTC TATTTAAATT CCTATACCAA CAAAAGGGAT 
GCCATAACTG CTGTACGGAA GATGAGGGCC CTGAATGGCT CAGCCCTGTA CACGGGATCT 
TCTCTGGACT TTGTTCGAAA CAACCTCTTC ACTAGCTCAG CCGGCCATAG GGCCGCTGAG 
GGGGTTCCTA AGCTCCTGGT GCTGATCACA GGCGGTAAGT CCCTCGATGA AGTCAGCCAG 
CCTGCCCAGG AGCTGAAGAG AGGCAGCATC ATGGCCTTGG CCGTTGGCAG CAAGGCCGCC 
GATGAGGATG AGCTGAAAGA GATCGCTTTC GACTCCTCCC TGGTCTTCAT TCCCGCTGAG 
TTCCGCCCTG CTCCCTTGCA AAACATGCTG CCCAGCCTGA TGGCACCTCT CAGGACTCTC 
ACTGGAACCA CGGAAGTTCA CGTAAACAAA CGGGATATCA TCTTCCTTTT GGATGGCTCC 
GACAACGTTG GGAAAAACAA TTTCCCTTAT GTGCGTGACT TTGTAACCAA CCTAGTTAAC 
AGCCTCGATG TTGGAAGTGA CAATATCCGT GTTGGCTTGG TGCAGTTTAG TGACACTCCG 
GTCACGGAGT TCTCTCTAGA CACTTACCAG ACCAAGTCCG AGCTGCTCGC TCACCTGAGG 
CGCTTGCAGC TCAAGGGGGG CTCCGGCTTG AACGCAGGCT CTGCCTTGAG CTATATCCAT 
GCCAATCACT TCACGGAGGC AGGAGGCAGC AGGACTCGTG AACACGTGCC TCAGCTGCTG 
CTCCTGCTCA TGGCCGGGCC CTCTGAGGAT GCCTATTTGC AAGCTGCCAA TGCCCTGGTG 
CGCTCAGGCG TGCTGACCTT CTGTGTGGGG ACCAATCGGG CGGATAAGGC TGAGTTAGAG 
CATATTGCTT TTAATCCCAG CCTGGTATAC CTCATGGACG ATTTCCGCTC CCTGCCATCT 
TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACATATGTTA GTGGAGGTGT ACAGGAAGTT 
CCACTATCCC AGCCAGAGAG CAAGCGGGAC ATTCTTTTCC TCTTTGACGG CTCAGTCAAT 
GTCTTGGGTC AGTTCCCTGC TGTCAGAGAC TTTCTCTACA GGATCATTGA GGAACTTGAT 
GTGAAGCCGG ATGGGACCCG GGTAGCAATA GCTCAGTTCA GTGATGACGT CAGGCTGGAG 
TCCCGCTTCA GTGAGCACCA GACGAAAGCT GAAATCCTTA ATCTCGTGAA GAAGATGAAG 
CTAAAAACAG GCAAAGCCCT CAACCTGGGC TACGCCCTGG ACTACGCGCT TAGGAACATC 
TTTGTGAGGT CGGCTGGCAG CCGCATCGAG GATAACGTGC AGCAGTTCCT GGTGCTGCTG 
GTTGCTGGAA GGTCGTCAGA TGCTGTGGCC GGGCCTGCAA GCTCCCTGAA GCAGAGAGGA 
GTGGTACCTT TCATCTTTCA AGCCAAGAAC GCCAACCCCA GTGAGCTGGA GCAGATTGTG 
CTGTCGCCTG CATTCATCCT GGCTGCAGAG TCACTCCCTA AGATCGGAGA CCTCCAATCA 
CAGATTGTGA GCCTTCTGAA AGCAGAACAG GGTTCAGGCC CTGTATCAGG TGAAAAGGAT 
GTGGTGTTTC TCATTGATGG CTCTGAGGGT GTCCGCAGTG GCTTCCCCCT GCTAAAGGAC 
TTTGTGCAGA GGGTTGTGGA GAGCTTGGAT GTGGGTCCCG ACCGTGTGCG TGTGGCACTG 
GTGCAGTACA GTGACCGGAC CAGGCCGGAG TTCTACCTGA ATTCCCACAT GGACCAGCAG 
GGTGTCATCA GCGCCATCCG CAGGCTGACA CTGCTGGGCG GCCCAACCCC CAACACAGGG 
GCGGCACTGG AGTTCGTGTT AAGGAACATT CTGACCAGTT CTACTGGGAG CAGGATAGCA 
GAAGGTGTTC CTCAGCTCCT GATTGTCCTC ACAGCAGAGC CATCAGGGGA TGACGTGCGA 
GGCCCTTCAG TAGTCCTGAA GCAGGGCGGG GCTGTGCCCA TTGGCATTGG CATTGGGAAT 
GCTGACATCT CTGAAATGCA GACCATCTCC TTCATCCCTG ACTTTGCTGT GGCCATCCCC 
ACCTTCCGGG AGCTTGGGAC GATACAGCAG GTCATCTCTG AGAGGGTGAT CCAGCTTAAT 
CGTGAGGAGC TGAGCTCGTT GAAACCCATT TTGACGCCCT CAACAGGTGC AGGTGTTGGC 
AGCAAGAAAG ATGTGGTCTT TCTCATCGAT GGATCCCGAA ATGCTGGCCC TGAGTTCCAG 
TACATCCGTA CCCTGATTGA GAGGATAGTC GAGTACCTGG ACATAGGCTT TGACACCACC 
CGGGTAGCCG TCATCCAGTT CAGTGAGGAC TCCAAGATGG AGTTTCCTCT GAATGCCCAC 
TTCAGCAAGG ATGAAGTACA GAACGCTGTG CGCAGGCTGC GGCCCAAGGG TGGGAGTCAG 
GTCTACATCG GAAATGCCCT GGAGTACGTG CTGAAGAACA TCTTCCAGAG GCCACTGGGG 
AGCCGGATAG AAGAGGGCGT TCCGCAGTTC CTAGTCCTCA TTTCGTCTGG AAAGTCTGAT 
GATGAGGTGG ACGACTCAGC CGTGGAACTC AAGCAGTTCG GTGTGGCCCC TCTCACCATA 
GCCAGACACA CAGACCAAGA GGAGCTGGTG AAGATCTCCC TAAGTCCTGA GTACGTGTAC 
TCCGTGAGCA CCTTCAGGGA GCTGCCCAGA CTGGAGCAGA AGCTGCTGAC GCCCATCACC 
ACTCTAACCT CACAGCAGAT CCATCAGATC CTGGCCAGTA CCCGATATCC CCCTTCAGTG 
GTTGAAAGCG ATGCGGCTGA CATCGTCTTT CTGATTGACA GCTCTGATGC CGTCAAGCCC 
GATGGCATCG CTCATATCCG AGACTTTGTC AGCAGGATCG TTCGCAGACT CAACATTGGT 
CCCAGTAAAG TGAGGATTGG GGTCGTGCAG TTTAGCAATG ATGTCTTCCC TGAGTTCTAC 
CTGAAGACCC ACAAGTCCCA GAGCAGTGTG CTTGAAGCCA TACGGCGTCT GAGGTTCAAA 
GGAGGGTCTC CCCTAAACAC TGGCAGAGCC CTGGAGTTTG TGGCAAGAAA CCTCTTCGTG 
AAGTCTGCTG GGAGCCGGAT AGAAGATGGG GTCCCTCAAC ACCTGGTCTT ATTCCTGGGT 
GGGAAGTCTC AGGACGATGT GGCCAGGCAT GCCCAAGTCA TAAGCTCATC GGGGATTGTG 
AGCTTAGGAA TAGGAGATCG GAACATTGAT AGGACGGACT TGCAAACCAT CACCAATGAC 
CCCAGGCTGG TCTTCACAGT TCGGGAATTC AGAGAGCTGC CCAACATAGA AGAGAGGGTC 
ATGCTTTCCT TTGGACCCTC TGGGGCTACT CCTCAGCCTC CTGGGGTAGA TTTACCTTCT 
CCTTCCCGGC CAGAGAAGAA GAAGGCCGAC ATTGTGTTCC TGCTGGATGG TTCCATCAAT 
TTTAGGAGGG ACAGTTTCCA GGAAGTGCTC CGTTTTGCAT CTGAAATCGT GGACACGGTC 
TATGAAGACG GAGATTCCAT CAGAGTGGGA CTGGTCCAGT ACAACTCAGA CCCCACGGAT 
GAATTCTTCC TGAGGGACTT TTCTACCAAA AGGCAGATTA TCGATGCCAT CAACAAAGTC 
GTCTATAAAG GAGGGAGGCA TGCCAACACC AGGGTGGGCA TCGAGCACCT GCTGCGGAAT 
CACTTTGTGC CGGAGGCTGG TAGCCGCCTG GATGAGCGGG TTCCTCAGAT CGCCTTCGTG 
ATCACCGGAG GGAAGTCAGT GGAGGATGCT CAGGATGTGA GCCTGGCTCT CACCCAGAAA 
GGTGTCAAGG TGTTTGCCGT TGGCGTGAGG AACATCGACT CTGAGGAGGT GGGCAAGATA 
GCCTCCAACA GTGCCACAGC CTTCCGGGTG GGCAGCGTGC AGGAACTGTC CGAACTGAGT 
GAGACAGTGC TCGAGACTCT GCATGATGCA ATGCATGAGA CCCTCTGCCC TGGTGTGACC 
GATGTCTCCA AAGCCTGCAA TCTGGAAGTC ATTCTGGGGT TTGATGGATC TCGAGATCAG 
AATGTGTTTG TGAGTCAGAA GGGCCTTGAG TCCAAGGTGG ACATCATCTT GAACAGAATC 
AGCCAGATAC AAAGGATCAG CTGCAGTGGC AACCAGCTGC CCACTGTGAG GGTATCCGTG 
ATGGCGAACA CGCCCTCTGG GCCAGTGGAG GCCTTTGACT TTGCCGAGTA TCAGCCGGAG 
CTGTTTGAGA AGTTTCGCAA CATGCGCAGT CAGCGCCCAT ACGTCCTCAC AGCTGACACG 
CTGAAGTTGT ACCAGAACAA GTTCAGGCAG TCGTCGCCTG ATACTGTGAA GGTAGTCATT 
CACTTCACGG ATGGAGCAGA TGGAGACATG GCTGACTTAT ACAGAGCATC TGAGGAACTC 
CGGCAAGCAG GTGCCCAAGC CCTGATCCTG GTGGGTCTTG AACGTGTGGC TAACCTGGAG 
CGGCTGATGC ATCTGGAGTT CGGGCGGGGC TTCATGTATG ACAGGCCCCT GAGGCTCAAC 
CTGCTTGACT TAGACTATGA ACTGGCAGAG CAGCTTGACA ATATTGCTGA GAAAGCTTGC 
TGTGGGGTTC CATGCAAGTG CTCTGGAGAG AGGGGAGACA GAGGGCCCAT TGGCAGCATT 
GGGCCAAAGG GTATCTCCGG GGAAGATGGC TACCGAGGCT ACCCGGGAGA CGAAGGCGGA 
CCTGGTGAGC GAGGTCCACC TGGTGTGAAT GGCACACAAG GTTTCCAGGG CTGTCCAGGC 
CAGAGGGGAG TGAAGGGTTC TCGAGGATTC CCAGGAGAGA AGGGTGAATT GGGAGAAATC 
GGCTTGGATG GTCTGGATGG TGAAGAGGGA GACAAGGGGC TGCCTGGTTC TTCTGGAGAA 
AAGGGGAGTC CTGGAAGAAG GGGTGACAAA GGACCCAAAG GAGACAAAGG CGAGAGAGGA 
GATGTCGGGA TTCGAGGTGA CCCGGGTGAC TCAGGACGGG ACAGCCAGCA GAGAGGACCC 
AAAGGAGAGA CGGGAGACAT CGGCCCCATG GGTCTCCCAG GCAGAGATGG CATCCCTGGC 
AGCCCAGGAG ACCCTGGGAA GGACGGTGGC TCTGGCCGAA GGGGCCCTGC AGGAGCTAAG 
GGCAACAGAG GTGGTCCTGG CCAGCCAGGC TTCGAAGGCG AGCAGGGTAC CAGAGGCTCA 
CAGGGCCCAC CTGGTCCCAT TGGTCCCCCA GGCCTGATTG GGGAACAAGG AATTCCCGGA 
CCTCGAGGAG GCGGAGGTAC GGCTGGGGCT CCTGGAGAAC GTGGCAGAAC TGGTCCTCTG 
GGGAGGAAGG GTGAGCCAGG AGAGCCAGGG CCGAAGGGAA GCATCGGGAA CCGTGGACCC 
CGAGGAGAGA CGGGAGATGA CGGGAGAGAT GGGGTTGGCA GTGAAGGACG CAGAGGCAAA 
AAAGGAGAAA GAGGCTTCCC TGGGTATCCG GGACCAAAGG GCACCCCTGG TGAGCCAGGG 
GCAGATGGAC CACCAGGACC CAAAGGCATC AGAGGTCGAA GGGGAAATTC AGGACCTCCA 
GGGGCAACTG GACAAAAGGG AGACCCTGGT TACCCAGGAC CATCTGGTCA CAAGGGCAAC 
CGAGGCGACT CCGTTGATCA ATGCGCCCTC ATCCAGAGCA TCAGAGACAA ATGCCCTTGC 
TGCTACGGGC CCCTGGAGTG CCCTGTATTC CCAACAGAAC TCGCCTTTGC CCTGGATACC 
TCTGAGGGGG TGACTCAGGA CACCTTCAGC CGGATGCGAG AGGTGCTTCT GGGCATTGTG 
GGCGATCTGA CCATTGCAGA GAGCAACTGT CCGCGGGGTG CCCGCGTGGC TGTGGTCACC 
TATAACAATG AGGTGACTAC AGAGATCCGG TTTGCTGACT CCAAGAAGAA GTCTGCCCTC 
TTAGACAGTA TTCAGAACCT CCAAGTGGCC TTGACCTCTA AGCAGCAGAG CCTGGAGACC 
GCAATGTCAT TCGTGGCCAG GAATACATTC AAACGTGTGA GGAGTGGCTT CCTGATGAGG 
AAAGTGGCTG TTTTCTTCAG CAACAAGCCC ACCAGGGCAT CCCCCCAGCT CCGAGAGGCC 
GTACTCAAGC TTTCCGATGC GGGGATCACA CCCTTGTTCC TTACAAGCCA GGAGGACCGA 
CAGCTCATCA ACGCTTTGCA GATCAATAAC ACAGCAGTGG GACATGCCCT GGTTCTGCCT 
GCAAGGAGGG ATCTCACAGA CTTCCTGAAG AATGTCCTTA CCTGCCATGT TTGCTTGGAC 
ATTTGCAATA TCGACCCATC CTGTGGCTTC GGCAGCTGGA GGCCTTCCTT CAGGGACCGG 
AGGGCAGCAG GCAGTGATGT GGACATAGAC CTGGCTTTCA TCTTGGACAG CTCAGAGGCT 
ACCACACTGT TCCAGTTCAA TGAGATGAAG AAATACATAG GCTATGTGAT CAGACAGCTG 
GACCTGAGCC CAGACCCGAA GGCTTCCCAA CACTTTGCCA GGGTGGCTGT TGTGCAGCAG 
TCAACCTACG AGTCTGTGGA CAATGCCAGT GTGCCGCCTG TGAAGGTGGA ATTCTCACTG 
ACAGACTATG GTGCCAAGGA AAAGCTGTTG GATTTCCTTA GCCGGAGGAT GACCCAACTT 
CAGGGAACCA TGGGCCTGGG CAATGCCATT GAATACACTA TAGAGAACAT CTTTGAAAGT 
GCTCCAAACC CACGGGACCT CAAAATTATG GTGCTGATGT TGACTGGTGA CATGCAGAGG 
CAGCAGCTGG AGGAGGCCCA GAGAGCCATC CTCCAGGCCA AGTGCAAGGG TTACTTCTTT 
GTGGTTCTGG GCATTGGCAG GAAGGTGAAT ATCAAGGAGG TCTACAGCTT TGCCAGTGAG 
CCCAATGATG TCTTCTTCAA ATTTGTGGAC AAATCAACTG AGCTCAACGA GGAGCCTCTG 
ATGCGCTTTG GGAGGCTGCT CCCATCCTTT GTCAGCAGTG AAAATGCATT TTACCTGCCT 
CCAGATCTCA GGAAACAGTG TGATTGGTTC CAAGGGGACC AACCAGTGAA GAATGGCGTG 
AAGTTTGGTC ACAAACAGAT AAACCCTCCA CATACTGCTA ATTCAAGTCT TACATCCAAA 
GTTGTGACCA CAATGAAGCC AGTGACAACG ACCAAACCAA CAGCAATTGT AAACCTGCCA 
CCTGCTAAGC CAGCCCCAGC GAGGCCAGCC CCTGCTCAGC CAGTTCTTGC TAAGCCAGAC 
CCCGCAAAAC CAGCCCAAGC AAGGCCAGCC CCTGCTAAGC CAGCTTCTGC TAAGTTGGTG 
CCTCCTCAGC CAGTCCATGT TCAGCCAGCC CCTGCCCAGA CAGCTTCTGT AAGACCAGCC 
CCCGCTAAGC CAGCCCCTCC TCAGCCAGCT GCTGCTAAGC CAGTCCCTGC TAAGCCAGCA 
GTCCCTGCTC AGCCAGCCCC TCCTCAGCCA GCTGCTGCTA AGCCAGTCCC TGCTAAGCCA 
GCAGTCCCTG CTCAGCCAGC CCCTCCTCAG CCAGCTGCTG CTAAGCCAGT CCCTGCTAAG 
CCAGCAGTCC CTGCTCAGCC AGCCGCTGCT CAACCAATGC CTGCTCAGCC AGTCCTTACT 
AAATCAGCCG CTGTGAAACC AGCCTCTGCC AACAAGCCTG TGGCAGCCAA ACCTGTGGCC 
ACGAACACAG CCACAGCCAC AGCCAGACCT GCATTAGCAG CGAAGCCTGC TGCAGCGAAG 
CCTGCAGCAA CGAGACCCCT TGCTGCTGCC ATAAGGCCAG TTGCTACCAA GCCAGAGGCC 
CCCAGGCAAC AGGCCAAACC AGCTGCCACC AAGCCAGCCA CTACTAAGCC CTTGGCGAGA 
GTGTCCCGAG AAGTCCAGGT CTCTGAAGTC ACGGAGAACA GTGCCAGACT CCACTGGGAG 
CGGCCCGAGC CTTCCAGCTC CTTTTTTTAT GACCTCACTG TAACCTCGGC CCACGACCAG 
TCCTTGGTTC TGAGACAAAA CCTCACTGTC ACTGACCGTG TCATCGGAGG CCTACTGGCT 
GGACAGCTGT ACCATGTCGT AGTGGTCTCC TACCTGCAGT CTCAGGTCAG GGCCATCTAC 
CAAGGAAGTT TCAATACGAA GAAAACCCAG CCTCCACCTC TTCAGGCAGC ACACCGAGCA 
TCCAGTTCAA CCATCAACCT CATGGTGAAC ACAGAACCAT TGTTTCTCAC TAAAACAGAT 
ATATGTAAGC TGTCCAGAGA TGCTGGGACT TGTGTGGACT TCAAGTTACT ATGGCACTAT 
GACCTAGAGA GCAAAAGTTG CAAGAGATTC TGGTATGGAG GTTGTGGAGG CAACGAGAAC 
AGATTCCACT CCCAGGAAGA ATGTGAAAAG ATGTGTAGTC CTGAGTTAAC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col6a3 ( XM_030245438.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101298.1 Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant X3, mRNA.

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CloneID	OMu84998
Clone ID Related Accession (Same CDS sequence)	XM_030245438.1
Accession Version	XM_030245438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-3(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAAC ACCGGCATCT GCCCCTAGTG GCTGTCTTTA GCCTTCTCCT CTCAGGCATT 
GCCACGACTC ATGCCCAACA GCATGGAGTC ATGGAAGTCA ACAAGAGGGA CATAGTCTTC 
CTGGTGGATG GTTCCTCCAG CCTGGGGCCA TCCAACTTCA ATGCCATCCG AGACTTCGTT 
ACCAGAGTCA TCCAGAGGTT GGAAATTGGA CAGGACCTGG TTCAGGTATC AGTGGCTCAG 
TATGCAGACA CTGTCAAGCC TGAGTTCTAT TTAAATTCCT ATACCAACAA AAGGGATGCC 
ATAACTGCTG TACGGAAGAT GAGGGCCCTG AATGGCTCAG CCCTGTACAC GGGATCTTCT 
CTGGACTTTG TTCGAAACAA CCTCTTCACT AGCTCAGCCG GCCATAGGGC CGCTGAGGGG 
GTTCCTAAGC TCCTGGTGCT GATCACAGGC GGTAAGTCCC TCGATGAAGT CAGCCAGCCT 
GCCCAGGAGC TGAAGAGAGG CAGCATCATG GCCTTGGCCG TTGGCAGCAA GGCCGCCGAT 
GAGGATGAGC TGAAAGAGAT CGCTTTCGAC TCCTCCCTGG TCTTCATTCC CGCTGAGTTC 
CGCCCTGCTC CCTTGCAAAA CATGCTGCCC AGCCTGATGG CACCTCTCAG GACTCTCACT 
GGAACCACGG AAGTTCACGT AAACAAACGG GATATCATCT TCCTTTTGGA TGGCTCCGAC 
AACGTTGGGA AAAACAATTT CCCTTATGTG CGTGACTTTG TAACCAACCT AGTTAACAGC 
CTCGATGTTG GAAGTGACAA TATCCGTGTT GGCTTGGTGC AGTTTAGTGA CACTCCGGTC 
ACGGAGTTCT CTCTAGACAC TTACCAGACC AAGTCCGAGC TGCTCGCTCA CCTGAGGCGC 
TTGCAGCTCA AGGGGGGCTC CGGCTTGAAC GCAGGCTCTG CCTTGAGCTA TATCCATGCC 
AATCACTTCA CGGAGGCAGG AGGCAGCAGG ACTCGTGAAC ACGTGCCTCA GCTGCTGCTC 
CTGCTCATGG CCGGGCCCTC TGAGGATGCC TATTTGCAAG CTGCCAATGC CCTGGTGCGC 
TCAGGCGTGC TGACCTTCTG TGTGGGGACC AATCGGGCGG ATAAGGCTGA GTTAGAGCAT 
ATTGCTTTTA ATCCCAGCCT GGTATACCTC ATGGACGATT TCCGCTCCCT GCCATCTTTG 
CCTCAACAGC TGATTCAGCC CCTAACCACA TATGTTAGTG GAGGTGTACA GGAAGTTCCA 
CTATCCCAGC CAGAGAGCAA GCGGGACATT CTTTTCCTCT TTGACGGCTC AGTCAATGTC 
TTGGGTCAGT TCCCTGCTGT CAGAGACTTT CTCTACAGGA TCATTGAGGA ACTTGATGTG 
AAGCCGGATG GGACCCGGGT AGCAATAGCT CAGTTCAGTG ATGACGTCAG GCTGGAGTCC 
CGCTTCAGTG AGCACCAGAC GAAAGCTGAA ATCCTTAATC TCGTGAAGAA GATGAAGCTA 
AAAACAGGCA AAGCCCTCAA CCTGGGCTAC GCCCTGGACT ACGCGCTTAG GAACATCTTT 
GTGAGGTCGG CTGGCAGCCG CATCGAGGAT AACGTGCAGC AGTTCCTGGT GCTGCTGGTT 
GCTGGAAGGT CGTCAGATGC TGTGGCCGGG CCTGCAAGCT CCCTGAAGCA GAGAGGAGTG 
GTACCTTTCA TCTTTCAAGC CAAGAACGCC AACCCCAGTG AGCTGGAGCA GATTGTGCTG 
TCGCCTGCAT TCATCCTGGC TGCAGAGTCA CTCCCTAAGA TCGGAGACCT CCAATCACAG 
ATTGTGAGCC TTCTGAAAGC AGAACAGGGT TCAGGCCCTG TATCAGGTGA AAAGGATGTG 
GTGTTTCTCA TTGATGGCTC TGAGGGTGTC CGCAGTGGCT TCCCCCTGCT AAAGGACTTT 
GTGCAGAGGG TTGTGGAGAG CTTGGATGTG GGTCCCGACC GTGTGCGTGT GGCACTGGTG 
CAGTACAGTG ACCGGACCAG GCCGGAGTTC TACCTGAATT CCCACATGGA CCAGCAGGGT 
GTCATCAGCG CCATCCGCAG GCTGACACTG CTGGGCGGCC CAACCCCCAA CACAGGGGCG 
GCACTGGAGT TCGTGTTAAG GAACATTCTG ACCAGTTCTA CTGGGAGCAG GATAGCAGAA 
GGTGTTCCTC AGCTCCTGAT TGTCCTCACA GCAGAGCCAT CAGGGGATGA CGTGCGAGGC 
CCTTCAGTAG TCCTGAAGCA GGGCGGGGCT GTGCCCATTG GCATTGGCAT TGGGAATGCT 
GACATCTCTG AAATGCAGAC CATCTCCTTC ATCCCTGACT TTGCTGTGGC CATCCCCACC 
TTCCGGGAGC TTGGGACGAT ACAGCAGGTC ATCTCTGAGA GGGTGATCCA GCTTAATCGT 
GAGGAGCTGA GCTCGTTGAA ACCCATTTTG ACGCCCTCAA CAGGTGCAGG TGTTGGCAGC 
AAGAAAGATG TGGTCTTTCT CATCGATGGA TCCCGAAATG CTGGCCCTGA GTTCCAGTAC 
ATCCGTACCC TGATTGAGAG GATAGTCGAG TACCTGGACA TAGGCTTTGA CACCACCCGG 
GTAGCCGTCA TCCAGTTCAG TGAGGACTCC AAGATGGAGT TTCCTCTGAA TGCCCACTTC 
AGCAAGGATG AAGTACAGAA CGCTGTGCGC AGGCTGCGGC CCAAGGGTGG GAGTCAGGTC 
TACATCGGAA ATGCCCTGGA GTACGTGCTG AAGAACATCT TCCAGAGGCC ACTGGGGAGC 
CGGATAGAAG AGGGCGTTCC GCAGTTCCTA GTCCTCATTT CGTCTGGAAA GTCTGATGAT 
GAGGTGGACG ACTCAGCCGT GGAACTCAAG CAGTTCGGTG TGGCCCCTCT CACCATAGCC 
AGACACACAG ACCAAGAGGA GCTGGTGAAG ATCTCCCTAA GTCCTGAGTA CGTGTACTCC 
GTGAGCACCT TCAGGGAGCT GCCCAGACTG GAGCAGAAGC TGCTGACGCC CATCACCACT 
CTAACCTCAC AGCAGATCCA TCAGATCCTG GCCAGTACCC GATATCCCCC TTCAGTGGTT 
GAAAGCGATG CGGCTGACAT CGTCTTTCTG ATTGACAGCT CTGATGCCGT CAAGCCCGAT 
GGCATCGCTC ATATCCGAGA CTTTGTCAGC AGGATCGTTC GCAGACTCAA CATTGGTCCC 
AGTAAAGTGA GGATTGGGGT CGTGCAGTTT AGCAATGATG TCTTCCCTGA GTTCTACCTG 
AAGACCCACA AGTCCCAGAG CAGTGTGCTT GAAGCCATAC GGCGTCTGAG GTTCAAAGGA 
GGGTCTCCCC TAAACACTGG CAGAGCCCTG GAGTTTGTGG CAAGAAACCT CTTCGTGAAG 
TCTGCTGGGA GCCGGATAGA AGATGGGGTC CCTCAACACC TGGTCTTATT CCTGGGTGGG 
AAGTCTCAGG ACGATGTGGC CAGGCATGCC CAAGTCATAA GCTCATCGGG GATTGTGAGC 
TTAGGAATAG GAGATCGGAA CATTGATAGG ACGGACTTGC AAACCATCAC CAATGACCCC 
AGGCTGGTCT TCACAGTTCG GGAATTCAGA GAGCTGCCCA ACATAGAAGA GAGGGTCATG 
CTTTCCTTTG GACCCTCTGG GGCTACTCCT CAGCCTCCTG GGGTAGATTT ACCTTCTCCT 
TCCCGGCCAG AGAAGAAGAA GGCCGACATT GTGTTCCTGC TGGATGGTTC CATCAATTTT 
AGGAGGGACA GTTTCCAGGA AGTGCTCCGT TTTGCATCTG AAATCGTGGA CACGGTCTAT 
GAAGACGGAG ATTCCATCAG AGTGGGACTG GTCCAGTACA ACTCAGACCC CACGGATGAA 
TTCTTCCTGA GGGACTTTTC TACCAAAAGG CAGATTATCG ATGCCATCAA CAAAGTCGTC 
TATAAAGGAG GGAGGCATGC CAACACCAGG GTGGGCATCG AGCACCTGCT GCGGAATCAC 
TTTGTGCCGG AGGCTGGTAG CCGCCTGGAT GAGCGGGTTC CTCAGATCGC CTTCGTGATC 
ACCGGAGGGA AGTCAGTGGA GGATGCTCAG GATGTGAGCC TGGCTCTCAC CCAGAAAGGT 
GTCAAGGTGT TTGCCGTTGG CGTGAGGAAC ATCGACTCTG AGGAGGTGGG CAAGATAGCC 
TCCAACAGTG CCACAGCCTT CCGGGTGGGC AGCGTGCAGG AACTGTCCGA ACTGAGTGAG 
ACAGTGCTCG AGACTCTGCA TGATGCAATG CATGAGACCC TCTGCCCTGG TGTGACCGAT 
GTCTCCAAAG CCTGCAATCT GGAAGTCATT CTGGGGTTTG ATGGATCTCG AGATCAGAAT 
GTGTTTGTGA GTCAGAAGGG CCTTGAGTCC AAGGTGGACA TCATCTTGAA CAGAATCAGC 
CAGATACAAA GGATCAGCTG CAGTGGCAAC CAGCTGCCCA CTGTGAGGGT ATCCGTGATG 
GCGAACACGC CCTCTGGGCC AGTGGAGGCC TTTGACTTTG CCGAGTATCA GCCGGAGCTG 
TTTGAGAAGT TTCGCAACAT GCGCAGTCAG CGCCCATACG TCCTCACAGC TGACACGCTG 
AAGTTGTACC AGAACAAGTT CAGGCAGTCG TCGCCTGATA CTGTGAAGGT AGTCATTCAC 
TTCACGGATG GAGCAGATGG AGACATGGCT GACTTATACA GAGCATCTGA GGAACTCCGG 
CAAGCAGGTG CCCAAGCCCT GATCCTGGTG GGTCTTGAAC GTGTGGCTAA CCTGGAGCGG 
CTGATGCATC TGGAGTTCGG GCGGGGCTTC ATGTATGACA GGCCCCTGAG GCTCAACCTG 
CTTGACTTAG ACTATGAACT GGCAGAGCAG CTTGACAATA TTGCTGAGAA AGCTTGCTGT 
GGGGTTCCAT GCAAGTGCTC TGGAGAGAGG GGAGACAGAG GGCCCATTGG CAGCATTGGG 
CCAAAGGGTA TCTCCGGGGA AGATGGCTAC CGAGGCTACC CGGGAGACGA AGGCGGACCT 
GGTGAGCGAG GTCCACCTGG TGTGAATGGC ACACAAGGTT TCCAGGGCTG TCCAGGCCAG 
AGGGGAGTGA AGGGTTCTCG AGGATTCCCA GGAGAGAAGG GTGAATTGGG AGAAATCGGC 
TTGGATGGTC TGGATGGTGA AGAGGGAGAC AAGGGGCTGC CTGGTTCTTC TGGAGAAAAG 
GGGAGTCCTG GAAGAAGGGG TGACAAAGGA CCCAAAGGAG ACAAAGGCGA GAGAGGAGAT 
GTCGGGATTC GAGGTGACCC GGGTGACTCA GGACGGGACA GCCAGCAGAG AGGACCCAAA 
GGAGAGACGG GAGACATCGG CCCCATGGGT CTCCCAGGCA GAGATGGCAT CCCTGGCAGC 
CCAGGAGACC CTGGGAAGGA CGGTGGCTCT GGCCGAAGGG GCCCTGCAGG AGCTAAGGGC 
AACAGAGGTG GTCCTGGCCA GCCAGGCTTC GAAGGCGAGC AGGGTACCAG AGGCTCACAG 
GGCCCACCTG GTCCCATTGG TCCCCCAGGC CTGATTGGGG AACAAGGAAT TCCCGGACCT 
CGAGGAGGCG GAGGTACGGC TGGGGCTCCT GGAGAACGTG GCAGAACTGG TCCTCTGGGG 
AGGAAGGGTG AGCCAGGAGA GCCAGGGCCG AAGGGAAGCA TCGGGAACCG TGGACCCCGA 
GGAGAGACGG GAGATGACGG GAGAGATGGG GTTGGCAGTG AAGGACGCAG AGGCAAAAAA 
GGAGAAAGAG GCTTCCCTGG GTATCCGGGA CCAAAGGGCA CCCCTGGTGA GCCAGGGGCA 
GATGGACCAC CAGGACCCAA AGGCATCAGA GGTCGAAGGG GAAATTCAGG ACCTCCAGGG 
GCAACTGGAC AAAAGGGAGA CCCTGGTTAC CCAGGACCAT CTGGTCACAA GGGCAACCGA 
GGCGACTCCG TTGATCAATG CGCCCTCATC CAGAGCATCA GAGACAAATG CCCTTGCTGC 
TACGGGCCCC TGGAGTGCCC TGTATTCCCA ACAGAACTCG CCTTTGCCCT GGATACCTCT 
GAGGGGGTGA CTCAGGACAC CTTCAGCCGG ATGCGAGAGG TGCTTCTGGG CATTGTGGGC 
GATCTGACCA TTGCAGAGAG CAACTGTCCG CGGGGTGCCC GCGTGGCTGT GGTCACCTAT 
AACAATGAGG TGACTACAGA GATCCGGTTT GCTGACTCCA AGAAGAAGTC TGCCCTCTTA 
GACAGTATTC AGAACCTCCA AGTGGCCTTG ACCTCTAAGC AGCAGAGCCT GGAGACCGCA 
ATGTCATTCG TGGCCAGGAA TACATTCAAA CGTGTGAGGA GTGGCTTCCT GATGAGGAAA 
GTGGCTGTTT TCTTCAGCAA CAAGCCCACC AGGGCATCCC CCCAGCTCCG AGAGGCCGTA 
CTCAAGCTTT CCGATGCGGG GATCACACCC TTGTTCCTTA CAAGCCAGGA GGACCGACAG 
CTCATCAACG CTTTGCAGAT CAATAACACA GCAGTGGGAC ATGCCCTGGT TCTGCCTGCA 
AGGAGGGATC TCACAGACTT CCTGAAGAAT GTCCTTACCT GCCATGTTTG CTTGGACATT 
TGCAATATCG ACCCATCCTG TGGCTTCGGC AGCTGGAGGC CTTCCTTCAG GGACCGGAGG 
GCAGCAGGCA GTGATGTGGA CATAGACCTG GCTTTCATCT TGGACAGCTC AGAGGCTACC 
ACACTGTTCC AGTTCAATGA GATGAAGAAA TACATAGGCT ATGTGATCAG ACAGCTGGAC 
CTGAGCCCAG ACCCGAAGGC TTCCCAACAC TTTGCCAGGG TGGCTGTTGT GCAGCAGTCA 
ACCTACGAGT CTGTGGACAA TGCCAGTGTG CCGCCTGTGA AGGTGGAATT CTCACTGACA 
GACTATGGTG CCAAGGAAAA GCTGTTGGAT TTCCTTAGCC GGAGGATGAC CCAACTTCAG 
GGAACCATGG GCCTGGGCAA TGCCATTGAA TACACTATAG AGAACATCTT TGAAAGTGCT 
CCAAACCCAC GGGACCTCAA AATTATGGTG CTGATGTTGA CTGGTGACAT GCAGAGGCAG 
CAGCTGGAGG AGGCCCAGAG AGCCATCCTC CAGGCCAAGT GCAAGGGTTA CTTCTTTGTG 
GTTCTGGGCA TTGGCAGGAA GGTGAATATC AAGGAGGTCT ACAGCTTTGC CAGTGAGCCC 
AATGATGTCT TCTTCAAATT TGTGGACAAA TCAACTGAGC TCAACGAGGA GCCTCTGATG 
CGCTTTGGGA GGCTGCTCCC ATCCTTTGTC AGCAGTGAAA ATGCATTTTA CCTGCCTCCA 
GATCTCAGGA AACAGTGTGA TTGGTTCCAA GGGGACCAAC CAGTGAAGAA TGGCGTGAAG 
TTTGGTCACA AACAGATAAA CCCTCCACAT ACTGCTAATT CAAGTCTTAC ATCCAAAGTT 
GTGACCACAA TGAAGCCAGT GACAACGACC AAACCAACAG CAATTGTAAA CCTGCCACCT 
GCTAAGCCAG CCCCAGCGAG GCCAGCCCCT GCTCAGCCAG TTCTTGCTAA GCCAGACCCC 
GCAAAACCAG CCCAAGCAAG GCCAGCCCCT GCTAAGCCAG CTTCTGCTAA GTTGGTGCCT 
CCTCAGCCAG TCCATGTTCA GCCAGCCCCT GCCCAGACAG CTTCTGTAAG ACCAGCCCCC 
GCTAAGCCAG CCCCTCCTCA GCCAGCTGCT GCTAAGCCAG TCCCTGCTAA GCCAGCAGTC 
CCTGCTCAGC CAGCCCCTCC TCAGCCAGCT GCTGCTAAGC CAGTCCCTGC TAAGCCAGCA 
GTCCCTGCTC AGCCAGCCCC TCCTCAGCCA GCTGCTGCTA AGCCAGTCCC TGCTAAGCCA 
GCAGTCCCTG CTCAGCCAGC CGCTGCTCAA CCAATGCCTG CTCAGCCAGT CCTTACTAAA 
TCAGCCGCTG TGAAACCAGC CTCTGCCAAC AAGCCTGTGG CAGCCAAACC TGTGGCCACG 
AACACAGCCA CAGCCACAGC CAGACCTGCA TTAGCAGCGA AGCCTGCTGC AGCGAAGCCT 
GCAGCAACGA GACCCCTTGC TGCTGCCATA AGGCCAGTTG CTACCAAGCC AGAGGCCCCC 
AGGCAACAGG CCAAACCAGC TGCCACCAAG CCAGCCACTA CTAAGCCCTT GGCGAGAGTG 
TCCCGAGAAG TCCAGGTCTC TGAAGTCACG GAGAACAGTG CCAGACTCCA CTGGGAGCGG 
CCCGAGCCTT CCAGCTCCTT TTTTTATGAC CTCACTGTAA CCTCGGCCCA CGACCAGTCC 
TTGGTTCTGA GACAAAACCT CACTGTCACT GACCGTGTCA TCGGAGGCCT ACTGGCTGGA 
CAGCTGTACC ATGTCGTAGT GGTCTCCTAC CTGCAGTCTC AGGTCAGGGC CATCTACCAA 
GGAAGTTTCA ATACGAAGAA AACCCAGCCT CCACCTCTTC AGGCAGCACA CCGAGCATCC 
AGTTCAACCA TCAACCTCAT GGTGAACACA GAACCATTGT TTCTCACTAA AACAGATATA 
TGTAAGCTGT CCAGAGATGC TGGGACTTGT GTGGACTTCA AGTTACTATG GCACTATGAC 
CTAGAGAGCA AAAGTTGCAA GAGATTCTGG TATGGAGGTT GTGGAGGCAA CGAGAACAGA 
TTCCACTCCC AGGAAGAATG TGAAAAGATG TGTAGTCCTG AGTTAACAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101298.1
CDS	281..8914
Translation	MRKHRHLPLVAVFSLLLSGIATTHAQQHGVMEVNKRDIVFLVDGSSSLGPSNFNAIRDFVTRVIQRLEIGQDLVQVSVAQYADTVKPEFYLNSYTNKRDAITAVRKMRALNGSALYTGSSLDFVRNNLFTSSAGHRAAEGVPKLLVLITGGKSLDEVSQPAQELKRGSIMALAVGSKAADEDELKEIAFDSSLVFIPAEFRPAPLQNMLPSLMAPLRTLTGTTEVHVNKRDIIFLLDGSDNVGKNNFPYVRDFVTNLVNSLDVGSDNIRVGLVQFSDTPVTEFSLDTYQTKSELLAHLRRLQLKGGSGLNAGSALSYIHANHFTEAGGSRTREHVPQLLLLLMAGPSEDAYLQAANALVRSGVLTFCVGTNRADKAELEHIAFNPSLVYLMDDFRSLPSLPQQLIQPLTTYVSGGVQEVPLSQPESKRDILFLFDGSVNVLGQFPAVRDFLYRIIEELDVKPDGTRVAIAQFSDDVRLESRFSEHQTKAEILNLVKKMKLKTGKALNLGYALDYALRNIFVRSAGSRIEDNVQQFLVLLVAGRSSDAVAGPASSLKQRGVVPFIFQAKNANPSELEQIVLSPAFILAAESLPKIGDLQSQIVSLLKAEQGSGPVSGEKDVVFLIDGSEGVRSGFPLLKDFVQRVVESLDVGPDRVRVALVQYSDRTRPEFYLNSHMDQQGVISAIRRLTLLGGPTPNTGAALEFVLRNILTSSTGSRIAEGVPQLLIVLTAEPSGDDVRGPSVVLKQGGAVPIGIGIGNADISEMQTISFIPDFAVAIPTFRELGTIQQVISERVIQLNREELSSLKPILTPSTGAGVGSKKDVVFLIDGSRNAGPEFQYIRTLIERIVEYLDIGFDTTRVAVIQFSEDSKMEFPLNAHFSKDEVQNAVRRLRPKGGSQVYIGNALEYVLKNIFQRPLGSRIEEGVPQFLVLISSGKSDDEVDDSAVELKQFGVAPLTIARHTDQEELVKISLSPEYVYSVSTFRELPRLEQKLLTPITTLTSQQIHQILASTRYPPSVVESDAADIVFLIDSSDAVKPDGIAHIRDFVSRIVRRLNIGPSKVRIGVVQFSNDVFPEFYLKTHKSQSSVLEAIRRLRFKGGSPLNTGRALEFVARNLFVKSAGSRIEDGVPQHLVLFLGGKSQDDVARHAQVISSSGIVSLGIGDRNIDRTDLQTITNDPRLVFTVREFRELPNIEERVMLSFGPSGATPQPPGVDLPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFASEIVDTVYEDGDSIRVGLVQYNSDPTDEFFLRDFSTKRQIIDAINKVVYKGGRHANTRVGIEHLLRNHFVPEAGSRLDERVPQIAFVITGGKSVEDAQDVSLALTQKGVKVFAVGVRNIDSEEVGKIASNSATAFRVGSVQELSELSETVLETLHDAMHETLCPGVTDVSKACNLEVILGFDGSRDQNVFVSQKGLESKVDIILNRISQIQRISCSGNQLPTVRVSVMANTPSGPVEAFDFAEYQPELFEKFRNMRSQRPYVLTADTLKLYQNKFRQSSPDTVKVVIHFTDGADGDMADLYRASEELRQAGAQALILVGLERVANLERLMHLEFGRGFMYDRPLRLNLLDLDYELAEQLDNIAEKACCGVPCKCSGERGDRGPIGSIGPKGISGEDGYRGYPGDEGGPGERGPPGVNGTQGFQGCPGQRGVKGSRGFPGEKGELGEIGLDGLDGEEGDKGLPGSSGEKGSPGRRGDKGPKGDKGERGDVGIRGDPGDSGRDSQQRGPKGETGDIGPMGLPGRDGIPGSPGDPGKDGGSGRRGPAGAKGNRGGPGQPGFEGEQGTRGSQGPPGPIGPPGLIGEQGIPGPRGGGGTAGAPGERGRTGPLGRKGEPGEPGPKGSIGNRGPRGETGDDGRDGVGSEGRRGKKGERGFPGYPGPKGTPGEPGADGPPGPKGIRGRRGNSGPPGATGQKGDPGYPGPSGHKGNRGDSVDQCALIQSIRDKCPCCYGPLECPVFPTELAFALDTSEGVTQDTFSRMREVLLGIVGDLTIAESNCPRGARVAVVTYNNEVTTEIRFADSKKKSALLDSIQNLQVALTSKQQSLETAMSFVARNTFKRVRSGFLMRKVAVFFSNKPTRASPQLREAVLKLSDAGITPLFLTSQEDRQLINALQINNTAVGHALVLPARRDLTDFLKNVLTCHVCLDICNIDPSCGFGSWRPSFRDRRAAGSDVDIDLAFILDSSEATTLFQFNEMKKYIGYVIRQLDLSPDPKASQHFARVAVVQQSTYESVDNASVPPVKVEFSLTDYGAKEKLLDFLSRRMTQLQGTMGLGNAIEYTIENIFESAPNPRDLKIMVLMLTGDMQRQQLEEAQRAILQAKCKGYFFVVLGIGRKVNIKEVYSFASEPNDVFFKFVDKSTELNEEPLMRFGRLLPSFVSSENAFYLPPDLRKQCDWFQGDQPVKNGVKFGHKQINPPHTANSSLTSKVVTTMKPVTTTKPTAIVNLPPAKPAPARPAPAQPVLAKPDPAKPAQARPAPAKPASAKLVPPQPVHVQPAPAQTASVRPAPAKPAPPQPAAAKPVPAKPAVPAQPAPPQPAAAKPVPAKPAVPAQPAPPQPAAAKPVPAKPAVPAQPAAAQPMPAQPVLTKSAAVKPASANKPVAAKPVATNTATATARPALAAKPAAAKPAATRPLAAAIRPVATKPEAPRQQAKPAATKPATTKPLARVSREVQVSEVTENSARLHWERPEPSSSFFYDLTVTSAHDQSLVLRQNLTVTDRVIGGLLAGQLYHVVVVSYLQSQVRAIYQGSFNTKKTQPPPLQAAHRASSSTINLMVNTEPLFLTKTDICKLSRDAGTCVDFKLLWHYDLESKSCKRFWYGGCGGNENRFHSQEECEKMCSPELTV

Target ORF information:

RefSeq Version	XM_030245438.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VI, alpha 3 (Col6a3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245438.1

ORF Insert Sequence:

ATGAGGAAAC ACCGGCATCT GCCCCTAGTG GCTGTCTTTA GCCTTCTCCT CTCAGGCATT 
GCCACGACTC ATGCCCAACA GCATGGAGTC ATGGAAGTCA ACAAGAGGGA CATAGTCTTC 
CTGGTGGATG GTTCCTCCAG CCTGGGGCCA TCCAACTTCA ATGCCATCCG AGACTTCGTT 
ACCAGAGTCA TCCAGAGGTT GGAAATTGGA CAGGACCTGG TTCAGGTATC AGTGGCTCAG 
TATGCAGACA CTGTCAAGCC TGAGTTCTAT TTAAATTCCT ATACCAACAA AAGGGATGCC 
ATAACTGCTG TACGGAAGAT GAGGGCCCTG AATGGCTCAG CCCTGTACAC GGGATCTTCT 
CTGGACTTTG TTCGAAACAA CCTCTTCACT AGCTCAGCCG GCCATAGGGC CGCTGAGGGG 
GTTCCTAAGC TCCTGGTGCT GATCACAGGC GGTAAGTCCC TCGATGAAGT CAGCCAGCCT 
GCCCAGGAGC TGAAGAGAGG CAGCATCATG GCCTTGGCCG TTGGCAGCAA GGCCGCCGAT 
GAGGATGAGC TGAAAGAGAT CGCTTTCGAC TCCTCCCTGG TCTTCATTCC CGCTGAGTTC 
CGCCCTGCTC CCTTGCAAAA CATGCTGCCC AGCCTGATGG CACCTCTCAG GACTCTCACT 
GGAACCACGG AAGTTCACGT AAACAAACGG GATATCATCT TCCTTTTGGA TGGCTCCGAC 
AACGTTGGGA AAAACAATTT CCCTTATGTG CGTGACTTTG TAACCAACCT AGTTAACAGC 
CTCGATGTTG GAAGTGACAA TATCCGTGTT GGCTTGGTGC AGTTTAGTGA CACTCCGGTC 
ACGGAGTTCT CTCTAGACAC TTACCAGACC AAGTCCGAGC TGCTCGCTCA CCTGAGGCGC 
TTGCAGCTCA AGGGGGGCTC CGGCTTGAAC GCAGGCTCTG CCTTGAGCTA TATCCATGCC 
AATCACTTCA CGGAGGCAGG AGGCAGCAGG ACTCGTGAAC ACGTGCCTCA GCTGCTGCTC 
CTGCTCATGG CCGGGCCCTC TGAGGATGCC TATTTGCAAG CTGCCAATGC CCTGGTGCGC 
TCAGGCGTGC TGACCTTCTG TGTGGGGACC AATCGGGCGG ATAAGGCTGA GTTAGAGCAT 
ATTGCTTTTA ATCCCAGCCT GGTATACCTC ATGGACGATT TCCGCTCCCT GCCATCTTTG 
CCTCAACAGC TGATTCAGCC CCTAACCACA TATGTTAGTG GAGGTGTACA GGAAGTTCCA 
CTATCCCAGC CAGAGAGCAA GCGGGACATT CTTTTCCTCT TTGACGGCTC AGTCAATGTC 
TTGGGTCAGT TCCCTGCTGT CAGAGACTTT CTCTACAGGA TCATTGAGGA ACTTGATGTG 
AAGCCGGATG GGACCCGGGT AGCAATAGCT CAGTTCAGTG ATGACGTCAG GCTGGAGTCC 
CGCTTCAGTG AGCACCAGAC GAAAGCTGAA ATCCTTAATC TCGTGAAGAA GATGAAGCTA 
AAAACAGGCA AAGCCCTCAA CCTGGGCTAC GCCCTGGACT ACGCGCTTAG GAACATCTTT 
GTGAGGTCGG CTGGCAGCCG CATCGAGGAT AACGTGCAGC AGTTCCTGGT GCTGCTGGTT 
GCTGGAAGGT CGTCAGATGC TGTGGCCGGG CCTGCAAGCT CCCTGAAGCA GAGAGGAGTG 
GTACCTTTCA TCTTTCAAGC CAAGAACGCC AACCCCAGTG AGCTGGAGCA GATTGTGCTG 
TCGCCTGCAT TCATCCTGGC TGCAGAGTCA CTCCCTAAGA TCGGAGACCT CCAATCACAG 
ATTGTGAGCC TTCTGAAAGC AGAACAGGGT TCAGGCCCTG TATCAGGTGA AAAGGATGTG 
GTGTTTCTCA TTGATGGCTC TGAGGGTGTC CGCAGTGGCT TCCCCCTGCT AAAGGACTTT 
GTGCAGAGGG TTGTGGAGAG CTTGGATGTG GGTCCCGACC GTGTGCGTGT GGCACTGGTG 
CAGTACAGTG ACCGGACCAG GCCGGAGTTC TACCTGAATT CCCACATGGA CCAGCAGGGT 
GTCATCAGCG CCATCCGCAG GCTGACACTG CTGGGCGGCC CAACCCCCAA CACAGGGGCG 
GCACTGGAGT TCGTGTTAAG GAACATTCTG ACCAGTTCTA CTGGGAGCAG GATAGCAGAA 
GGTGTTCCTC AGCTCCTGAT TGTCCTCACA GCAGAGCCAT CAGGGGATGA CGTGCGAGGC 
CCTTCAGTAG TCCTGAAGCA GGGCGGGGCT GTGCCCATTG GCATTGGCAT TGGGAATGCT 
GACATCTCTG AAATGCAGAC CATCTCCTTC ATCCCTGACT TTGCTGTGGC CATCCCCACC 
TTCCGGGAGC TTGGGACGAT ACAGCAGGTC ATCTCTGAGA GGGTGATCCA GCTTAATCGT 
GAGGAGCTGA GCTCGTTGAA ACCCATTTTG ACGCCCTCAA CAGGTGCAGG TGTTGGCAGC 
AAGAAAGATG TGGTCTTTCT CATCGATGGA TCCCGAAATG CTGGCCCTGA GTTCCAGTAC 
ATCCGTACCC TGATTGAGAG GATAGTCGAG TACCTGGACA TAGGCTTTGA CACCACCCGG 
GTAGCCGTCA TCCAGTTCAG TGAGGACTCC AAGATGGAGT TTCCTCTGAA TGCCCACTTC 
AGCAAGGATG AAGTACAGAA CGCTGTGCGC AGGCTGCGGC CCAAGGGTGG GAGTCAGGTC 
TACATCGGAA ATGCCCTGGA GTACGTGCTG AAGAACATCT TCCAGAGGCC ACTGGGGAGC 
CGGATAGAAG AGGGCGTTCC GCAGTTCCTA GTCCTCATTT CGTCTGGAAA GTCTGATGAT 
GAGGTGGACG ACTCAGCCGT GGAACTCAAG CAGTTCGGTG TGGCCCCTCT CACCATAGCC 
AGACACACAG ACCAAGAGGA GCTGGTGAAG ATCTCCCTAA GTCCTGAGTA CGTGTACTCC 
GTGAGCACCT TCAGGGAGCT GCCCAGACTG GAGCAGAAGC TGCTGACGCC CATCACCACT 
CTAACCTCAC AGCAGATCCA TCAGATCCTG GCCAGTACCC GATATCCCCC TTCAGTGGTT 
GAAAGCGATG CGGCTGACAT CGTCTTTCTG ATTGACAGCT CTGATGCCGT CAAGCCCGAT 
GGCATCGCTC ATATCCGAGA CTTTGTCAGC AGGATCGTTC GCAGACTCAA CATTGGTCCC 
AGTAAAGTGA GGATTGGGGT CGTGCAGTTT AGCAATGATG TCTTCCCTGA GTTCTACCTG 
AAGACCCACA AGTCCCAGAG CAGTGTGCTT GAAGCCATAC GGCGTCTGAG GTTCAAAGGA 
GGGTCTCCCC TAAACACTGG CAGAGCCCTG GAGTTTGTGG CAAGAAACCT CTTCGTGAAG 
TCTGCTGGGA GCCGGATAGA AGATGGGGTC CCTCAACACC TGGTCTTATT CCTGGGTGGG 
AAGTCTCAGG ACGATGTGGC CAGGCATGCC CAAGTCATAA GCTCATCGGG GATTGTGAGC 
TTAGGAATAG GAGATCGGAA CATTGATAGG ACGGACTTGC AAACCATCAC CAATGACCCC 
AGGCTGGTCT TCACAGTTCG GGAATTCAGA GAGCTGCCCA ACATAGAAGA GAGGGTCATG 
CTTTCCTTTG GACCCTCTGG GGCTACTCCT CAGCCTCCTG GGGTAGATTT ACCTTCTCCT 
TCCCGGCCAG AGAAGAAGAA GGCCGACATT GTGTTCCTGC TGGATGGTTC CATCAATTTT 
AGGAGGGACA GTTTCCAGGA AGTGCTCCGT TTTGCATCTG AAATCGTGGA CACGGTCTAT 
GAAGACGGAG ATTCCATCAG AGTGGGACTG GTCCAGTACA ACTCAGACCC CACGGATGAA 
TTCTTCCTGA GGGACTTTTC TACCAAAAGG CAGATTATCG ATGCCATCAA CAAAGTCGTC 
TATAAAGGAG GGAGGCATGC CAACACCAGG GTGGGCATCG AGCACCTGCT GCGGAATCAC 
TTTGTGCCGG AGGCTGGTAG CCGCCTGGAT GAGCGGGTTC CTCAGATCGC CTTCGTGATC 
ACCGGAGGGA AGTCAGTGGA GGATGCTCAG GATGTGAGCC TGGCTCTCAC CCAGAAAGGT 
GTCAAGGTGT TTGCCGTTGG CGTGAGGAAC ATCGACTCTG AGGAGGTGGG CAAGATAGCC 
TCCAACAGTG CCACAGCCTT CCGGGTGGGC AGCGTGCAGG AACTGTCCGA ACTGAGTGAG 
ACAGTGCTCG AGACTCTGCA TGATGCAATG CATGAGACCC TCTGCCCTGG TGTGACCGAT 
GTCTCCAAAG CCTGCAATCT GGAAGTCATT CTGGGGTTTG ATGGATCTCG AGATCAGAAT 
GTGTTTGTGA GTCAGAAGGG CCTTGAGTCC AAGGTGGACA TCATCTTGAA CAGAATCAGC 
CAGATACAAA GGATCAGCTG CAGTGGCAAC CAGCTGCCCA CTGTGAGGGT ATCCGTGATG 
GCGAACACGC CCTCTGGGCC AGTGGAGGCC TTTGACTTTG CCGAGTATCA GCCGGAGCTG 
TTTGAGAAGT TTCGCAACAT GCGCAGTCAG CGCCCATACG TCCTCACAGC TGACACGCTG 
AAGTTGTACC AGAACAAGTT CAGGCAGTCG TCGCCTGATA CTGTGAAGGT AGTCATTCAC 
TTCACGGATG GAGCAGATGG AGACATGGCT GACTTATACA GAGCATCTGA GGAACTCCGG 
CAAGCAGGTG CCCAAGCCCT GATCCTGGTG GGTCTTGAAC GTGTGGCTAA CCTGGAGCGG 
CTGATGCATC TGGAGTTCGG GCGGGGCTTC ATGTATGACA GGCCCCTGAG GCTCAACCTG 
CTTGACTTAG ACTATGAACT GGCAGAGCAG CTTGACAATA TTGCTGAGAA AGCTTGCTGT 
GGGGTTCCAT GCAAGTGCTC TGGAGAGAGG GGAGACAGAG GGCCCATTGG CAGCATTGGG 
CCAAAGGGTA TCTCCGGGGA AGATGGCTAC CGAGGCTACC CGGGAGACGA AGGCGGACCT 
GGTGAGCGAG GTCCACCTGG TGTGAATGGC ACACAAGGTT TCCAGGGCTG TCCAGGCCAG 
AGGGGAGTGA AGGGTTCTCG AGGATTCCCA GGAGAGAAGG GTGAATTGGG AGAAATCGGC 
TTGGATGGTC TGGATGGTGA AGAGGGAGAC AAGGGGCTGC CTGGTTCTTC TGGAGAAAAG 
GGGAGTCCTG GAAGAAGGGG TGACAAAGGA CCCAAAGGAG ACAAAGGCGA GAGAGGAGAT 
GTCGGGATTC GAGGTGACCC GGGTGACTCA GGACGGGACA GCCAGCAGAG AGGACCCAAA 
GGAGAGACGG GAGACATCGG CCCCATGGGT CTCCCAGGCA GAGATGGCAT CCCTGGCAGC 
CCAGGAGACC CTGGGAAGGA CGGTGGCTCT GGCCGAAGGG GCCCTGCAGG AGCTAAGGGC 
AACAGAGGTG GTCCTGGCCA GCCAGGCTTC GAAGGCGAGC AGGGTACCAG AGGCTCACAG 
GGCCCACCTG GTCCCATTGG TCCCCCAGGC CTGATTGGGG AACAAGGAAT TCCCGGACCT 
CGAGGAGGCG GAGGTACGGC TGGGGCTCCT GGAGAACGTG GCAGAACTGG TCCTCTGGGG 
AGGAAGGGTG AGCCAGGAGA GCCAGGGCCG AAGGGAAGCA TCGGGAACCG TGGACCCCGA 
GGAGAGACGG GAGATGACGG GAGAGATGGG GTTGGCAGTG AAGGACGCAG AGGCAAAAAA 
GGAGAAAGAG GCTTCCCTGG GTATCCGGGA CCAAAGGGCA CCCCTGGTGA GCCAGGGGCA 
GATGGACCAC CAGGACCCAA AGGCATCAGA GGTCGAAGGG GAAATTCAGG ACCTCCAGGG 
GCAACTGGAC AAAAGGGAGA CCCTGGTTAC CCAGGACCAT CTGGTCACAA GGGCAACCGA 
GGCGACTCCG TTGATCAATG CGCCCTCATC CAGAGCATCA GAGACAAATG CCCTTGCTGC 
TACGGGCCCC TGGAGTGCCC TGTATTCCCA ACAGAACTCG CCTTTGCCCT GGATACCTCT 
GAGGGGGTGA CTCAGGACAC CTTCAGCCGG ATGCGAGAGG TGCTTCTGGG CATTGTGGGC 
GATCTGACCA TTGCAGAGAG CAACTGTCCG CGGGGTGCCC GCGTGGCTGT GGTCACCTAT 
AACAATGAGG TGACTACAGA GATCCGGTTT GCTGACTCCA AGAAGAAGTC TGCCCTCTTA 
GACAGTATTC AGAACCTCCA AGTGGCCTTG ACCTCTAAGC AGCAGAGCCT GGAGACCGCA 
ATGTCATTCG TGGCCAGGAA TACATTCAAA CGTGTGAGGA GTGGCTTCCT GATGAGGAAA 
GTGGCTGTTT TCTTCAGCAA CAAGCCCACC AGGGCATCCC CCCAGCTCCG AGAGGCCGTA 
CTCAAGCTTT CCGATGCGGG GATCACACCC TTGTTCCTTA CAAGCCAGGA GGACCGACAG 
CTCATCAACG CTTTGCAGAT CAATAACACA GCAGTGGGAC ATGCCCTGGT TCTGCCTGCA 
AGGAGGGATC TCACAGACTT CCTGAAGAAT GTCCTTACCT GCCATGTTTG CTTGGACATT 
TGCAATATCG ACCCATCCTG TGGCTTCGGC AGCTGGAGGC CTTCCTTCAG GGACCGGAGG 
GCAGCAGGCA GTGATGTGGA CATAGACCTG GCTTTCATCT TGGACAGCTC AGAGGCTACC 
ACACTGTTCC AGTTCAATGA GATGAAGAAA TACATAGGCT ATGTGATCAG ACAGCTGGAC 
CTGAGCCCAG ACCCGAAGGC TTCCCAACAC TTTGCCAGGG TGGCTGTTGT GCAGCAGTCA 
ACCTACGAGT CTGTGGACAA TGCCAGTGTG CCGCCTGTGA AGGTGGAATT CTCACTGACA 
GACTATGGTG CCAAGGAAAA GCTGTTGGAT TTCCTTAGCC GGAGGATGAC CCAACTTCAG 
GGAACCATGG GCCTGGGCAA TGCCATTGAA TACACTATAG AGAACATCTT TGAAAGTGCT 
CCAAACCCAC GGGACCTCAA AATTATGGTG CTGATGTTGA CTGGTGACAT GCAGAGGCAG 
CAGCTGGAGG AGGCCCAGAG AGCCATCCTC CAGGCCAAGT GCAAGGGTTA CTTCTTTGTG 
GTTCTGGGCA TTGGCAGGAA GGTGAATATC AAGGAGGTCT ACAGCTTTGC CAGTGAGCCC 
AATGATGTCT TCTTCAAATT TGTGGACAAA TCAACTGAGC TCAACGAGGA GCCTCTGATG 
CGCTTTGGGA GGCTGCTCCC ATCCTTTGTC AGCAGTGAAA ATGCATTTTA CCTGCCTCCA 
GATCTCAGGA AACAGTGTGA TTGGTTCCAA GGGGACCAAC CAGTGAAGAA TGGCGTGAAG 
TTTGGTCACA AACAGATAAA CCCTCCACAT ACTGCTAATT CAAGTCTTAC ATCCAAAGTT 
GTGACCACAA TGAAGCCAGT GACAACGACC AAACCAACAG CAATTGTAAA CCTGCCACCT 
GCTAAGCCAG CCCCAGCGAG GCCAGCCCCT GCTCAGCCAG TTCTTGCTAA GCCAGACCCC 
GCAAAACCAG CCCAAGCAAG GCCAGCCCCT GCTAAGCCAG CTTCTGCTAA GTTGGTGCCT 
CCTCAGCCAG TCCATGTTCA GCCAGCCCCT GCCCAGACAG CTTCTGTAAG ACCAGCCCCC 
GCTAAGCCAG CCCCTCCTCA GCCAGCTGCT GCTAAGCCAG TCCCTGCTAA GCCAGCAGTC 
CCTGCTCAGC CAGCCCCTCC TCAGCCAGCT GCTGCTAAGC CAGTCCCTGC TAAGCCAGCA 
GTCCCTGCTC AGCCAGCCCC TCCTCAGCCA GCTGCTGCTA AGCCAGTCCC TGCTAAGCCA 
GCAGTCCCTG CTCAGCCAGC CGCTGCTCAA CCAATGCCTG CTCAGCCAGT CCTTACTAAA 
TCAGCCGCTG TGAAACCAGC CTCTGCCAAC AAGCCTGTGG CAGCCAAACC TGTGGCCACG 
AACACAGCCA CAGCCACAGC CAGACCTGCA TTAGCAGCGA AGCCTGCTGC AGCGAAGCCT 
GCAGCAACGA GACCCCTTGC TGCTGCCATA AGGCCAGTTG CTACCAAGCC AGAGGCCCCC 
AGGCAACAGG CCAAACCAGC TGCCACCAAG CCAGCCACTA CTAAGCCCTT GGCGAGAGTG 
TCCCGAGAAG TCCAGGTCTC TGAAGTCACG GAGAACAGTG CCAGACTCCA CTGGGAGCGG 
CCCGAGCCTT CCAGCTCCTT TTTTTATGAC CTCACTGTAA CCTCGGCCCA CGACCAGTCC 
TTGGTTCTGA GACAAAACCT CACTGTCACT GACCGTGTCA TCGGAGGCCT ACTGGCTGGA 
CAGCTGTACC ATGTCGTAGT GGTCTCCTAC CTGCAGTCTC AGGTCAGGGC CATCTACCAA 
GGAAGTTTCA ATACGAAGAA AACCCAGCCT CCACCTCTTC AGGCAGCACA CCGAGCATCC 
AGTTCAACCA TCAACCTCAT GGTGAACACA GAACCATTGT TTCTCACTAA AACAGATATA 
TGTAAGCTGT CCAGAGATGC TGGGACTTGT GTGGACTTCA AGTTACTATG GCACTATGAC 
CTAGAGAGCA AAAGTTGCAA GAGATTCTGG TATGGAGGTT GTGGAGGCAA CGAGAACAGA 
TTCCACTCCC AGGAAGAATG TGAAAAGATG TGTAGTCCTG AGTTAACAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

Divergent functions of endotrophin on different cell populations in adipose tissue.
American journal of physiology. Endocrinology and metabolism311(6)E952-E963(2016 Dec)
Zhao Y,Gu X,Zhang N,Kolonin MG,An Z,Sun K

A collagen VI-dependent pathogenic mechanism for Hirschsprung's disease.
The Journal of clinical investigation125(12)4483-96(2015 Dec)
Soret R,Mennetrey M,Bergeron KF,Dariel A,Neunlist M,Grunder F,Faure C,Silversides DW,Pilon N,

Regulatory mechanisms of anthrax toxin receptor 1-dependent vascular and connective tissue homeostasis.
Matrix biology : journal of the International Society for Matrix Biology4256-73(2015 Mar)
Besschetnova TY,Ichimura T,Katebi N,St Croix B,Bonventre JV,Olsen BR

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Col6a3 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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