Cep250 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_017315701.1	XP_017171190.1	centrosome-associated protein CEP250 isoform X6
NM_177217.3	NP_796191.2	centrosome-associated protein CEP250 isoform 1
NM_008383.3	NP_032409.3	centrosome-associated protein CEP250 isoform 1
XM_030247663.1	XP_030103523.1	centrosome-associated protein CEP250 isoform X4
NM_001130000.1	NP_001123472.1	centrosome-associated protein CEP250 isoform 3
XM_006498796.1	XP_006498859.1	centrosome-associated protein CEP250 isoform X1
XM_006498798.1	XP_006498861.1	centrosome-associated protein CEP250 isoform X2
XM_030247666.1	XP_030103526.1	centrosome-associated protein CEP250 isoform X4
XM_030247667.1	XP_030103527.1	centrosome-associated protein CEP250 isoform X4
XM_006498802.4	XP_006498865.1	centrosome-associated protein CEP250 isoform X5
NM_001129999.1	NP_001123471.1	centrosome-associated protein CEP250 isoform 2
XM_006498797.3	XP_006498860.1	centrosome-associated protein CEP250 isoform X1
XM_006498799.2	XP_006498862.1	centrosome-associated protein CEP250 isoform X3
XM_006498802.3	XP_006498865.1	centrosome-associated protein CEP250 isoform X4
XM_006498797.4	XP_006498860.1	centrosome-associated protein CEP250 isoform X1
XM_006498799.3	XP_006498862.1	centrosome-associated protein CEP250 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu30045	XM_006498802.4 Latest version!	Mus musculus centrosomal protein 250 (Cep250), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price
OMu06037	XM_006498797.4 Latest version!	Mus musculus centrosomal protein 250 (Cep250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	Quote Price
OMu30043	XM_006498799.3 Latest version!	Mus musculus centrosomal protein 250 (Cep250), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	Quote Price
OMu87726	XM_030247667.1 Latest version!	Mus musculus centrosomal protein 250 (Cep250), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	Quote Price
OMu87726	XM_030247666.1 Latest version!	Mus musculus centrosomal protein 250 (Cep250), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	Quote Price
OMu87726	XM_030247663.1 Latest version!	Mus musculus centrosomal protein 250 (Cep250), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	Quote Price
OMu01820	NM_008383.3 Latest version!	Mus musculus centrosomal protein 250 (Cep250), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu01820	NM_177217.3 Latest version!	Mus musculus centrosomal protein 250 (Cep250), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu06035	NM_001129999.1 Latest version!	Mus musculus centrosomal protein 250 (Cep250), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu06037	NM_001130000.1 Latest version!	Mus musculus centrosomal protein 250 (Cep250), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu06037	XM_006498796.1 Latest version!	Mus musculus centrosomal protein 250 (Cep250), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu30042	XM_006498798.1 Latest version!	Mus musculus centrosomal protein 250 (Cep250), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu80076	XM_017315701.1 Latest version!	Mus musculus centrosomal protein 250 (Cep250), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OMu30045	XM_006498802.3	Mus musculus centrosomal protein 250 (Cep250), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
OMu06037	XM_006498797.3	Mus musculus centrosomal protein 250 (Cep250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu30043	XM_006498799.2	Mus musculus centrosomal protein 250 (Cep250), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

Cep250 ( XM_006498802.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498865.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu30045
Clone ID Related Accession (Same CDS sequence)	XM_006498802.4 , XM_006498802.3
Accession Version	XM_006498802.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498802.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGGG GCTCAGGGGA AGTGAAATGC CTGAAGCTGG AACTGGATGC TGAGAGGACC 
CGGGCAGAGC AGGAGTGGGA TGCAGTAGCC AGGCAGCTAG CCCAAGCTGA GCAGGAGGGG 
CAAGCATCTC TGGAGCGACA GAAGGTGGCC CACGAGGAGG AGGTGAACAG GCTGCAAGAG 
AAATGGGAGA AAGAGCGCTC CTGGCTGCAG CAGGAGCTGG ATAAGACCCT GGAAACCCTC 
GAGAGGGAGA GAGCAGAGCT GGAGACAAAG CTGAGAGAGC AGCAGACAGA AATGGAAGCC 
ATCCGTGCAC AGAGGGAAGA GGAACGAAGC CAGGCTGACA GCGCCCTGTA TCAAATGCAG 
CTAGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGAGGAC CCAGAAGGAG 
CTGGCAGATG CTAGTCAACA GCTGGAAAGG CTGAGACAGG ACATGAAGAT TCAGAAGTTA 
AAGGAACAGG AGACCACTGG CATGCTGCAG GCCCAGCTTC AGGAAACACA GCAGGAGCTG 
AAGGAGGCAG CCCAGCAGCA CAGGGACGAC CTTGCTGCCT TCCAGAAGGA CAAGCTGGAC 
CTGCAGAAGC AGGTAGAGGA CCTAATGTCT CAGCTGGTAG CCCACGATGA CTCCCAGAGG 
CTGGTGAAAG AAGAGATTGA AGAGAAGGTG AAAGTGGCTC AAGAATGCAG CCGGATTCAG 
AAGGAACTGG AGAAAGAGAA CGCCAGCCTG GCTCTGTCGC TGGTGGAAAA GGAAAAACGA 
CTTCTCATTT TACAAGAAGC TGACTCTGTA AGACAGCAGG AGCTGAGCTC CTTGCGCCAG 
GACATACAGG AGGCCCAGGA AGGGCAAAGA GAGCTGGGTG TACAGGTGGA ACTATTGAGG 
CAGGAGGTGA AGGAAAAGGA GGCTGACTTT GTGGCCCGGG AAGCACAGCT GCTGGAGGAG 
TTGGAGGCCT CACGGGTTGC AGAGCAGCAA CTTCGAGCGT CTTTGTGGGC CCAGGAAGCC 
AAGGCAACCC AGCTGCAGCT GCAGCTGCGC AGCACTGAGA GCCAGCTAGA GGCATTGGTT 
GCAGAACAAC AGCCGGAGAA CCAGGCACAG GCACAGCTAG CTAGCCTCTG CTCTGTTCTG 
CAGCAGGCCC TGGGGTCTGC ATGTGAGAGT AGGCCTGAGC TGAGGGGTGG AGGCGACTCT 
GCTCCTACCT TATGGGGCCC TGATCCAGAT CAGAATGGAG CTTCCAGACT CTTTAAGAGA 
TGGTCGCTCC CAACGGCACT GTCACCGGAG GCTGTGGCAC TAGCTCTCCA GAAGCTCCAT 
CAGGACGTGT GGAAGGCTCG GCAGGCCCGG GATGACCTAA GGGACCAGGT CCAGAAGCTG 
GTGCAGCGCC TGACTGACAC GGAGGCCCAG AAGAGCCAGG TTCACTCGGA GCTGCAGGAC 
CTGCAGAGAC AGCTCTCTCA GAGCCAGGAA GAGAAATCCA AGTGGGAAGG GAGGCAGAAC 
TCCCTGGAGT CTGAGCTGAG GGACTTGCAC GAGACCGCGG CATCCTTACA GAGTCGCCTC 
CGGCAGGCAG AACTGCAAAA AATGGAAGCT CAGAATGATC GAGAGTTGCT TCAGGCATCC 
AAGGAGAAGC TGTCAGCCCA GGTTGAACAT CTGCAAGCAT GTGTTGCAGA AGCCCAGGCT 
CAGGCAGATG CAGCTGCTGT CCTGGAAGAA GATCTGCGAA CTGCTCGCTC TGCACTGAAA 
CTGAAAAACG AGGAGCTAGA GAGTGAACGG GAGAGAGCCC AGGCTTTACA AGAACAGGGT 
GAGCTGAAGG TGGCTCAGGG GAAAGCTTTG CAGGAGAACC TAGCCCTGCT GGCACAGACC 
CTGTCTAACA GAGAGCGGGA GGTCGAGACG TTGCAGGCTG AGGTTCAGGA ACTGGAGAAG 
CAGCGGGAGA TGCAGAAGGC TGCTCTGGAG CTGCTCTCCC TCGACCTGAA GAAGCGCAGT 
CGGGAGGTAG ACCTGCAACA AGAACAGATT CAGGAGCTGG AGCAGTGCAG GTCAGTCTTA 
GAGCACTTGC CCATGGCCGT CCAAGAGCGA GAGCAGAAAC TGAGTGTGCA GCGGGACCAG 
ATCAGGGAAC TGGAGAACGA TCGGGAGGCC CAGAGGAGTG TCCTGGAACA CCAGCTTCTG 
GACCTGGAGC AGAAGGCTCA AGTGATTGAG TCCCAGAGGG GACAGATTCA GGATCTGAAA 
AAGCAACTGG GGACACTGGA ATGCCTGGCC CTGGAACTGG AGGAAAGCCA TCACAAAGTG 
GAGAGCCAAC AGAAGATGAT CACAGAACTA GAGGGCCAGA GGGAAATGCA GAGGGTGGCT 
CTAACCCACC TCACACTGGA CCTGGAAGAA AGGAGCCAGG AGCTGCAGGC ACAAAGCAGC 
CAGCTCCATG AGCTGGAGAA CCATAGCACC CATCTGGCAA AAGAGCTGCA GGAGAGGGAC 
CAGGAGGTGA CGTCCCAACG CCAGCAGATC GATGAGCTGC AGAAGCAGCA GGAACAGCTG 
GCTCAGGCCC TGGAGAGGAA AGGCCAGGAG CTGGTGCTGC AGAAGGAAAG GATTCAGGTT 
CTGGAAGATC AGAGGACGCT GCAGACCAAG ATCTTAGAGG AGGACCTAGA ACAGATCAAG 
CATTCCTTGA GAGAACGCAG CCAGGAGCTG GCCTCTCAGT GGCAGCTGGT GCATGAGCGT 
GCCGACGATG GAAAGAGCCC GAGTAAAGGC CAGCGAGGGA GCCTGGAGCA CTTGAAGCTG 
ATCCTGCGTG ATAAGGAGAA GGAGGTAGAG TGCCAGCAGG AGCGCATCCA GGAACTTCAG 
GGCCACATGG GCCAGCTGGA ACAGCAGCTG CAGGGTCTGC ACAGGAAGGT GGGGGAGACT 
AGCCTGCTCC TGACCCATCG AGAGCAGGAG ACAGCAACCC TGCAGCAGCA TCTCCAGGAA 
GCCAAGGAAC AAGGGGAACT GAGAGAGCAG GTACTTCAGG GTCAGCTGGA GGAGGCCCAG 
AGAGATCTGG CCCAGAGGGA CCATGAACTA GAGACCCTCC GGCAGGAAAA ACAGCAGACC 
CAGGACCAGG AGGAAAGCAT GAAGCTAAAG ACAAGTGCTC TGCAGGCCGC TCTGGAGCAG 
GCCCATGCGA CACTGAAGGA GCGTCAGGGG GAGCTGGAAG AACACAGGGA ACAGGTGCGA 
AGGCTCCAGG AAGAACTAGA AGTGGAGGGA CGGCAGGTAC GGGCCCTGGA GGAGGTGTTG 
GGGGACCTAA GGGCTGAGTC TCGGGAGCAC GAGAAGGCTG TGCTGGCCCT TCAACAACGG 
TGTGCTGAGC AGGCCCAGGA ACATGAGGCA GAAGCCAGGA CCCTGCAGGA CAGCTGGCTG 
CAGGCCCAGG CCACTCTCAC AGAACAAGAA CAAGAGCTAG CGGCACTGCG AGCGGAAAAC 
CAGTATTCCC GCCGACAGGA AGAGGCTGCA GTTAGCCAGG CAGAGGCGCT GCAGGAGGCC 
CTCAGCAAAG CCCAGGCTGC CCTGCAGGAG AAAGAGCAGA GTCTGCTTGA ACAGGCTGAG 
TTGAGCCACA CCCTGGAGGC CAGCACTGCC GCCTTGCAAG CTACCCTGGA CACCTGCCAG 
GCAAGTGCCC GGCAGCTGGA GGAGGCCCTG AGGATTCGGG AAGGTGAGAT CCAGGCCCAG 
GCTCTCCAAC ACCACGAGGT CACGCAGCAC CTCCAGCAGG AACTTTGCCA GAAGAAAGAA 
GAGCTGAGGC AGCTACTAGA GAAGGCTGGG GCCCGGAGGA GTCAAGAAAA TGGAATCCAA 
GAGAAGCAAA GTCTGGAGCA AGAGAGACAA GAGGAAACAA GGAGGCTCCT TGAGAGCCTA 
AAGGAGCTAC AGCTGACTGT AGCCCAAAGG GAAGAAGAGA TTCTGATGCT GAGGGAGGCC 
TCGTCCCCAC GCCACAGAGC CCTCCCAGCC GAGAAGCCAG CTCTGCAACC GCTCCCAGCA 
CAACAGGAGT TAGAGCGGCT GCAGACTGCC CTAAGGCAAA CAGAGGCCAG GGAGATTGAA 
TGGAGGGAGA AGGCTCAGGA CCTGGCTCTG TCCCTGGCCC AAAGCAAGGC CAGCATCAGC 
AGTCTGCAGG AGATAACCAT GTTCCTACAA GCCTCTGTTC TAGAGCGGGA ATCAGAACAA 
CAAAGACTAC AGGAGGAGTT GGTACTCAGC AGACAAGCTC TGGAAGAGCA GCAGTCAGGT 
GGCCCACACT CAACCAGCAG AGCAGACCAG GGACCAAAAG TGGGACAGGG AAGCCAGTCT 
GGAGAGGTAG AGACTGAGCC TAGTCCTGGA GTGGAGGAGA AGGAACGGTT GACACAGAGG 
CTTGAACGTC TGCAGCAAGC GGTGGCAGAG CTGGAAGTTG ACCGAAGCAA GCTGCAGTGC 
CACAATGCGC AGCTGCGGAC CGCCTTGGAG CAGGTGGAAC GAGAACGGAG GAAGCTGAAG 
AGGGATTCCG TGCGTGCATC CCGGGCAGGA TCACTGGAGG CCAGAGAAAC TATGACTTCA 
TCCCCCACAC AGCAGGATGG GAGAGGCAGT CAGAGGGGTT CCTCGGACAG CGTGCTTGTG 
GTTGAGCTTC AGAGAGAGGT AGCCCTGCTG CGAGCCCAGC TGGCCTTGGA GCGAAAGCAG 
AGGCAGGACT ACATTGCACG GTCCGTGCAG ACCAGCCGTG AGCTAGCAGG CCTGCATCAC 
AGCCTGTCTC ACTCCCTCCT CACCGTGGCC CAGGCCCCTG AGGCCACTGT TCTGGAGGCT 
GAGACCCGAA AGCTGGATGA GTCTCTGAAT CAGAGTCTGA CATCCCCAGG GCCATGTTTG 
CTACATCCCA GTCTGGACAC TACTCAAAAT ACCCACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498865.1
CDS	65..4906
Translation	MSWGSGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	XM_006498802.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498802.4

ORF Insert Sequence:

ATGTCCTGGG GCTCAGGGGA AGTGAAATGC CTGAAGCTGG AACTGGATGC TGAGAGGACC 
CGGGCAGAGC AGGAGTGGGA TGCAGTAGCC AGGCAGCTAG CCCAAGCTGA GCAGGAGGGG 
CAAGCATCTC TGGAGCGACA GAAGGTGGCC CACGAGGAGG AGGTGAACAG GCTGCAAGAG 
AAATGGGAGA AAGAGCGCTC CTGGCTGCAG CAGGAGCTGG ATAAGACCCT GGAAACCCTC 
GAGAGGGAGA GAGCAGAGCT GGAGACAAAG CTGAGAGAGC AGCAGACAGA AATGGAAGCC 
ATCCGTGCAC AGAGGGAAGA GGAACGAAGC CAGGCTGACA GCGCCCTGTA TCAAATGCAG 
CTAGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGAGGAC CCAGAAGGAG 
CTGGCAGATG CTAGTCAACA GCTGGAAAGG CTGAGACAGG ACATGAAGAT TCAGAAGTTA 
AAGGAACAGG AGACCACTGG CATGCTGCAG GCCCAGCTTC AGGAAACACA GCAGGAGCTG 
AAGGAGGCAG CCCAGCAGCA CAGGGACGAC CTTGCTGCCT TCCAGAAGGA CAAGCTGGAC 
CTGCAGAAGC AGGTAGAGGA CCTAATGTCT CAGCTGGTAG CCCACGATGA CTCCCAGAGG 
CTGGTGAAAG AAGAGATTGA AGAGAAGGTG AAAGTGGCTC AAGAATGCAG CCGGATTCAG 
AAGGAACTGG AGAAAGAGAA CGCCAGCCTG GCTCTGTCGC TGGTGGAAAA GGAAAAACGA 
CTTCTCATTT TACAAGAAGC TGACTCTGTA AGACAGCAGG AGCTGAGCTC CTTGCGCCAG 
GACATACAGG AGGCCCAGGA AGGGCAAAGA GAGCTGGGTG TACAGGTGGA ACTATTGAGG 
CAGGAGGTGA AGGAAAAGGA GGCTGACTTT GTGGCCCGGG AAGCACAGCT GCTGGAGGAG 
TTGGAGGCCT CACGGGTTGC AGAGCAGCAA CTTCGAGCGT CTTTGTGGGC CCAGGAAGCC 
AAGGCAACCC AGCTGCAGCT GCAGCTGCGC AGCACTGAGA GCCAGCTAGA GGCATTGGTT 
GCAGAACAAC AGCCGGAGAA CCAGGCACAG GCACAGCTAG CTAGCCTCTG CTCTGTTCTG 
CAGCAGGCCC TGGGGTCTGC ATGTGAGAGT AGGCCTGAGC TGAGGGGTGG AGGCGACTCT 
GCTCCTACCT TATGGGGCCC TGATCCAGAT CAGAATGGAG CTTCCAGACT CTTTAAGAGA 
TGGTCGCTCC CAACGGCACT GTCACCGGAG GCTGTGGCAC TAGCTCTCCA GAAGCTCCAT 
CAGGACGTGT GGAAGGCTCG GCAGGCCCGG GATGACCTAA GGGACCAGGT CCAGAAGCTG 
GTGCAGCGCC TGACTGACAC GGAGGCCCAG AAGAGCCAGG TTCACTCGGA GCTGCAGGAC 
CTGCAGAGAC AGCTCTCTCA GAGCCAGGAA GAGAAATCCA AGTGGGAAGG GAGGCAGAAC 
TCCCTGGAGT CTGAGCTGAG GGACTTGCAC GAGACCGCGG CATCCTTACA GAGTCGCCTC 
CGGCAGGCAG AACTGCAAAA AATGGAAGCT CAGAATGATC GAGAGTTGCT TCAGGCATCC 
AAGGAGAAGC TGTCAGCCCA GGTTGAACAT CTGCAAGCAT GTGTTGCAGA AGCCCAGGCT 
CAGGCAGATG CAGCTGCTGT CCTGGAAGAA GATCTGCGAA CTGCTCGCTC TGCACTGAAA 
CTGAAAAACG AGGAGCTAGA GAGTGAACGG GAGAGAGCCC AGGCTTTACA AGAACAGGGT 
GAGCTGAAGG TGGCTCAGGG GAAAGCTTTG CAGGAGAACC TAGCCCTGCT GGCACAGACC 
CTGTCTAACA GAGAGCGGGA GGTCGAGACG TTGCAGGCTG AGGTTCAGGA ACTGGAGAAG 
CAGCGGGAGA TGCAGAAGGC TGCTCTGGAG CTGCTCTCCC TCGACCTGAA GAAGCGCAGT 
CGGGAGGTAG ACCTGCAACA AGAACAGATT CAGGAGCTGG AGCAGTGCAG GTCAGTCTTA 
GAGCACTTGC CCATGGCCGT CCAAGAGCGA GAGCAGAAAC TGAGTGTGCA GCGGGACCAG 
ATCAGGGAAC TGGAGAACGA TCGGGAGGCC CAGAGGAGTG TCCTGGAACA CCAGCTTCTG 
GACCTGGAGC AGAAGGCTCA AGTGATTGAG TCCCAGAGGG GACAGATTCA GGATCTGAAA 
AAGCAACTGG GGACACTGGA ATGCCTGGCC CTGGAACTGG AGGAAAGCCA TCACAAAGTG 
GAGAGCCAAC AGAAGATGAT CACAGAACTA GAGGGCCAGA GGGAAATGCA GAGGGTGGCT 
CTAACCCACC TCACACTGGA CCTGGAAGAA AGGAGCCAGG AGCTGCAGGC ACAAAGCAGC 
CAGCTCCATG AGCTGGAGAA CCATAGCACC CATCTGGCAA AAGAGCTGCA GGAGAGGGAC 
CAGGAGGTGA CGTCCCAACG CCAGCAGATC GATGAGCTGC AGAAGCAGCA GGAACAGCTG 
GCTCAGGCCC TGGAGAGGAA AGGCCAGGAG CTGGTGCTGC AGAAGGAAAG GATTCAGGTT 
CTGGAAGATC AGAGGACGCT GCAGACCAAG ATCTTAGAGG AGGACCTAGA ACAGATCAAG 
CATTCCTTGA GAGAACGCAG CCAGGAGCTG GCCTCTCAGT GGCAGCTGGT GCATGAGCGT 
GCCGACGATG GAAAGAGCCC GAGTAAAGGC CAGCGAGGGA GCCTGGAGCA CTTGAAGCTG 
ATCCTGCGTG ATAAGGAGAA GGAGGTAGAG TGCCAGCAGG AGCGCATCCA GGAACTTCAG 
GGCCACATGG GCCAGCTGGA ACAGCAGCTG CAGGGTCTGC ACAGGAAGGT GGGGGAGACT 
AGCCTGCTCC TGACCCATCG AGAGCAGGAG ACAGCAACCC TGCAGCAGCA TCTCCAGGAA 
GCCAAGGAAC AAGGGGAACT GAGAGAGCAG GTACTTCAGG GTCAGCTGGA GGAGGCCCAG 
AGAGATCTGG CCCAGAGGGA CCATGAACTA GAGACCCTCC GGCAGGAAAA ACAGCAGACC 
CAGGACCAGG AGGAAAGCAT GAAGCTAAAG ACAAGTGCTC TGCAGGCCGC TCTGGAGCAG 
GCCCATGCGA CACTGAAGGA GCGTCAGGGG GAGCTGGAAG AACACAGGGA ACAGGTGCGA 
AGGCTCCAGG AAGAACTAGA AGTGGAGGGA CGGCAGGTAC GGGCCCTGGA GGAGGTGTTG 
GGGGACCTAA GGGCTGAGTC TCGGGAGCAC GAGAAGGCTG TGCTGGCCCT TCAACAACGG 
TGTGCTGAGC AGGCCCAGGA ACATGAGGCA GAAGCCAGGA CCCTGCAGGA CAGCTGGCTG 
CAGGCCCAGG CCACTCTCAC AGAACAAGAA CAAGAGCTAG CGGCACTGCG AGCGGAAAAC 
CAGTATTCCC GCCGACAGGA AGAGGCTGCA GTTAGCCAGG CAGAGGCGCT GCAGGAGGCC 
CTCAGCAAAG CCCAGGCTGC CCTGCAGGAG AAAGAGCAGA GTCTGCTTGA ACAGGCTGAG 
TTGAGCCACA CCCTGGAGGC CAGCACTGCC GCCTTGCAAG CTACCCTGGA CACCTGCCAG 
GCAAGTGCCC GGCAGCTGGA GGAGGCCCTG AGGATTCGGG AAGGTGAGAT CCAGGCCCAG 
GCTCTCCAAC ACCACGAGGT CACGCAGCAC CTCCAGCAGG AACTTTGCCA GAAGAAAGAA 
GAGCTGAGGC AGCTACTAGA GAAGGCTGGG GCCCGGAGGA GTCAAGAAAA TGGAATCCAA 
GAGAAGCAAA GTCTGGAGCA AGAGAGACAA GAGGAAACAA GGAGGCTCCT TGAGAGCCTA 
AAGGAGCTAC AGCTGACTGT AGCCCAAAGG GAAGAAGAGA TTCTGATGCT GAGGGAGGCC 
TCGTCCCCAC GCCACAGAGC CCTCCCAGCC GAGAAGCCAG CTCTGCAACC GCTCCCAGCA 
CAACAGGAGT TAGAGCGGCT GCAGACTGCC CTAAGGCAAA CAGAGGCCAG GGAGATTGAA 
TGGAGGGAGA AGGCTCAGGA CCTGGCTCTG TCCCTGGCCC AAAGCAAGGC CAGCATCAGC 
AGTCTGCAGG AGATAACCAT GTTCCTACAA GCCTCTGTTC TAGAGCGGGA ATCAGAACAA 
CAAAGACTAC AGGAGGAGTT GGTACTCAGC AGACAAGCTC TGGAAGAGCA GCAGTCAGGT 
GGCCCACACT CAACCAGCAG AGCAGACCAG GGACCAAAAG TGGGACAGGG AAGCCAGTCT 
GGAGAGGTAG AGACTGAGCC TAGTCCTGGA GTGGAGGAGA AGGAACGGTT GACACAGAGG 
CTTGAACGTC TGCAGCAAGC GGTGGCAGAG CTGGAAGTTG ACCGAAGCAA GCTGCAGTGC 
CACAATGCGC AGCTGCGGAC CGCCTTGGAG CAGGTGGAAC GAGAACGGAG GAAGCTGAAG 
AGGGATTCCG TGCGTGCATC CCGGGCAGGA TCACTGGAGG CCAGAGAAAC TATGACTTCA 
TCCCCCACAC AGCAGGATGG GAGAGGCAGT CAGAGGGGTT CCTCGGACAG CGTGCTTGTG 
GTTGAGCTTC AGAGAGAGGT AGCCCTGCTG CGAGCCCAGC TGGCCTTGGA GCGAAAGCAG 
AGGCAGGACT ACATTGCACG GTCCGTGCAG ACCAGCCGTG AGCTAGCAGG CCTGCATCAC 
AGCCTGTCTC ACTCCCTCCT CACCGTGGCC CAGGCCCCTG AGGCCACTGT TCTGGAGGCT 
GAGACCCGAA AGCTGGATGA GTCTCTGAAT CAGAGTCTGA CATCCCCAGG GCCATGTTTG 
CTACATCCCA GTCTGGACAC TACTCAAAAT ACCCACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( XM_006498802.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498865.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant X5, mRNA.

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CloneID	OMu30045
Clone ID Related Accession (Same CDS sequence)	XM_006498802.4 , XM_006498802.3
Accession Version	XM_006498802.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498802.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGGG GCTCAGGGGA AGTGAAATGC CTGAAGCTGG AACTGGATGC TGAGAGGACC 
CGGGCAGAGC AGGAGTGGGA TGCAGTAGCC AGGCAGCTAG CCCAAGCTGA GCAGGAGGGG 
CAAGCATCTC TGGAGCGACA GAAGGTGGCC CACGAGGAGG AGGTGAACAG GCTGCAAGAG 
AAATGGGAGA AAGAGCGCTC CTGGCTGCAG CAGGAGCTGG ATAAGACCCT GGAAACCCTC 
GAGAGGGAGA GAGCAGAGCT GGAGACAAAG CTGAGAGAGC AGCAGACAGA AATGGAAGCC 
ATCCGTGCAC AGAGGGAAGA GGAACGAAGC CAGGCTGACA GCGCCCTGTA TCAAATGCAG 
CTAGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGAGGAC CCAGAAGGAG 
CTGGCAGATG CTAGTCAACA GCTGGAAAGG CTGAGACAGG ACATGAAGAT TCAGAAGTTA 
AAGGAACAGG AGACCACTGG CATGCTGCAG GCCCAGCTTC AGGAAACACA GCAGGAGCTG 
AAGGAGGCAG CCCAGCAGCA CAGGGACGAC CTTGCTGCCT TCCAGAAGGA CAAGCTGGAC 
CTGCAGAAGC AGGTAGAGGA CCTAATGTCT CAGCTGGTAG CCCACGATGA CTCCCAGAGG 
CTGGTGAAAG AAGAGATTGA AGAGAAGGTG AAAGTGGCTC AAGAATGCAG CCGGATTCAG 
AAGGAACTGG AGAAAGAGAA CGCCAGCCTG GCTCTGTCGC TGGTGGAAAA GGAAAAACGA 
CTTCTCATTT TACAAGAAGC TGACTCTGTA AGACAGCAGG AGCTGAGCTC CTTGCGCCAG 
GACATACAGG AGGCCCAGGA AGGGCAAAGA GAGCTGGGTG TACAGGTGGA ACTATTGAGG 
CAGGAGGTGA AGGAAAAGGA GGCTGACTTT GTGGCCCGGG AAGCACAGCT GCTGGAGGAG 
TTGGAGGCCT CACGGGTTGC AGAGCAGCAA CTTCGAGCGT CTTTGTGGGC CCAGGAAGCC 
AAGGCAACCC AGCTGCAGCT GCAGCTGCGC AGCACTGAGA GCCAGCTAGA GGCATTGGTT 
GCAGAACAAC AGCCGGAGAA CCAGGCACAG GCACAGCTAG CTAGCCTCTG CTCTGTTCTG 
CAGCAGGCCC TGGGGTCTGC ATGTGAGAGT AGGCCTGAGC TGAGGGGTGG AGGCGACTCT 
GCTCCTACCT TATGGGGCCC TGATCCAGAT CAGAATGGAG CTTCCAGACT CTTTAAGAGA 
TGGTCGCTCC CAACGGCACT GTCACCGGAG GCTGTGGCAC TAGCTCTCCA GAAGCTCCAT 
CAGGACGTGT GGAAGGCTCG GCAGGCCCGG GATGACCTAA GGGACCAGGT CCAGAAGCTG 
GTGCAGCGCC TGACTGACAC GGAGGCCCAG AAGAGCCAGG TTCACTCGGA GCTGCAGGAC 
CTGCAGAGAC AGCTCTCTCA GAGCCAGGAA GAGAAATCCA AGTGGGAAGG GAGGCAGAAC 
TCCCTGGAGT CTGAGCTGAG GGACTTGCAC GAGACCGCGG CATCCTTACA GAGTCGCCTC 
CGGCAGGCAG AACTGCAAAA AATGGAAGCT CAGAATGATC GAGAGTTGCT TCAGGCATCC 
AAGGAGAAGC TGTCAGCCCA GGTTGAACAT CTGCAAGCAT GTGTTGCAGA AGCCCAGGCT 
CAGGCAGATG CAGCTGCTGT CCTGGAAGAA GATCTGCGAA CTGCTCGCTC TGCACTGAAA 
CTGAAAAACG AGGAGCTAGA GAGTGAACGG GAGAGAGCCC AGGCTTTACA AGAACAGGGT 
GAGCTGAAGG TGGCTCAGGG GAAAGCTTTG CAGGAGAACC TAGCCCTGCT GGCACAGACC 
CTGTCTAACA GAGAGCGGGA GGTCGAGACG TTGCAGGCTG AGGTTCAGGA ACTGGAGAAG 
CAGCGGGAGA TGCAGAAGGC TGCTCTGGAG CTGCTCTCCC TCGACCTGAA GAAGCGCAGT 
CGGGAGGTAG ACCTGCAACA AGAACAGATT CAGGAGCTGG AGCAGTGCAG GTCAGTCTTA 
GAGCACTTGC CCATGGCCGT CCAAGAGCGA GAGCAGAAAC TGAGTGTGCA GCGGGACCAG 
ATCAGGGAAC TGGAGAACGA TCGGGAGGCC CAGAGGAGTG TCCTGGAACA CCAGCTTCTG 
GACCTGGAGC AGAAGGCTCA AGTGATTGAG TCCCAGAGGG GACAGATTCA GGATCTGAAA 
AAGCAACTGG GGACACTGGA ATGCCTGGCC CTGGAACTGG AGGAAAGCCA TCACAAAGTG 
GAGAGCCAAC AGAAGATGAT CACAGAACTA GAGGGCCAGA GGGAAATGCA GAGGGTGGCT 
CTAACCCACC TCACACTGGA CCTGGAAGAA AGGAGCCAGG AGCTGCAGGC ACAAAGCAGC 
CAGCTCCATG AGCTGGAGAA CCATAGCACC CATCTGGCAA AAGAGCTGCA GGAGAGGGAC 
CAGGAGGTGA CGTCCCAACG CCAGCAGATC GATGAGCTGC AGAAGCAGCA GGAACAGCTG 
GCTCAGGCCC TGGAGAGGAA AGGCCAGGAG CTGGTGCTGC AGAAGGAAAG GATTCAGGTT 
CTGGAAGATC AGAGGACGCT GCAGACCAAG ATCTTAGAGG AGGACCTAGA ACAGATCAAG 
CATTCCTTGA GAGAACGCAG CCAGGAGCTG GCCTCTCAGT GGCAGCTGGT GCATGAGCGT 
GCCGACGATG GAAAGAGCCC GAGTAAAGGC CAGCGAGGGA GCCTGGAGCA CTTGAAGCTG 
ATCCTGCGTG ATAAGGAGAA GGAGGTAGAG TGCCAGCAGG AGCGCATCCA GGAACTTCAG 
GGCCACATGG GCCAGCTGGA ACAGCAGCTG CAGGGTCTGC ACAGGAAGGT GGGGGAGACT 
AGCCTGCTCC TGACCCATCG AGAGCAGGAG ACAGCAACCC TGCAGCAGCA TCTCCAGGAA 
GCCAAGGAAC AAGGGGAACT GAGAGAGCAG GTACTTCAGG GTCAGCTGGA GGAGGCCCAG 
AGAGATCTGG CCCAGAGGGA CCATGAACTA GAGACCCTCC GGCAGGAAAA ACAGCAGACC 
CAGGACCAGG AGGAAAGCAT GAAGCTAAAG ACAAGTGCTC TGCAGGCCGC TCTGGAGCAG 
GCCCATGCGA CACTGAAGGA GCGTCAGGGG GAGCTGGAAG AACACAGGGA ACAGGTGCGA 
AGGCTCCAGG AAGAACTAGA AGTGGAGGGA CGGCAGGTAC GGGCCCTGGA GGAGGTGTTG 
GGGGACCTAA GGGCTGAGTC TCGGGAGCAC GAGAAGGCTG TGCTGGCCCT TCAACAACGG 
TGTGCTGAGC AGGCCCAGGA ACATGAGGCA GAAGCCAGGA CCCTGCAGGA CAGCTGGCTG 
CAGGCCCAGG CCACTCTCAC AGAACAAGAA CAAGAGCTAG CGGCACTGCG AGCGGAAAAC 
CAGTATTCCC GCCGACAGGA AGAGGCTGCA GTTAGCCAGG CAGAGGCGCT GCAGGAGGCC 
CTCAGCAAAG CCCAGGCTGC CCTGCAGGAG AAAGAGCAGA GTCTGCTTGA ACAGGCTGAG 
TTGAGCCACA CCCTGGAGGC CAGCACTGCC GCCTTGCAAG CTACCCTGGA CACCTGCCAG 
GCAAGTGCCC GGCAGCTGGA GGAGGCCCTG AGGATTCGGG AAGGTGAGAT CCAGGCCCAG 
GCTCTCCAAC ACCACGAGGT CACGCAGCAC CTCCAGCAGG AACTTTGCCA GAAGAAAGAA 
GAGCTGAGGC AGCTACTAGA GAAGGCTGGG GCCCGGAGGA GTCAAGAAAA TGGAATCCAA 
GAGAAGCAAA GTCTGGAGCA AGAGAGACAA GAGGAAACAA GGAGGCTCCT TGAGAGCCTA 
AAGGAGCTAC AGCTGACTGT AGCCCAAAGG GAAGAAGAGA TTCTGATGCT GAGGGAGGCC 
TCGTCCCCAC GCCACAGAGC CCTCCCAGCC GAGAAGCCAG CTCTGCAACC GCTCCCAGCA 
CAACAGGAGT TAGAGCGGCT GCAGACTGCC CTAAGGCAAA CAGAGGCCAG GGAGATTGAA 
TGGAGGGAGA AGGCTCAGGA CCTGGCTCTG TCCCTGGCCC AAAGCAAGGC CAGCATCAGC 
AGTCTGCAGG AGATAACCAT GTTCCTACAA GCCTCTGTTC TAGAGCGGGA ATCAGAACAA 
CAAAGACTAC AGGAGGAGTT GGTACTCAGC AGACAAGCTC TGGAAGAGCA GCAGTCAGGT 
GGCCCACACT CAACCAGCAG AGCAGACCAG GGACCAAAAG TGGGACAGGG AAGCCAGTCT 
GGAGAGGTAG AGACTGAGCC TAGTCCTGGA GTGGAGGAGA AGGAACGGTT GACACAGAGG 
CTTGAACGTC TGCAGCAAGC GGTGGCAGAG CTGGAAGTTG ACCGAAGCAA GCTGCAGTGC 
CACAATGCGC AGCTGCGGAC CGCCTTGGAG CAGGTGGAAC GAGAACGGAG GAAGCTGAAG 
AGGGATTCCG TGCGTGCATC CCGGGCAGGA TCACTGGAGG CCAGAGAAAC TATGACTTCA 
TCCCCCACAC AGCAGGATGG GAGAGGCAGT CAGAGGGGTT CCTCGGACAG CGTGCTTGTG 
GTTGAGCTTC AGAGAGAGGT AGCCCTGCTG CGAGCCCAGC TGGCCTTGGA GCGAAAGCAG 
AGGCAGGACT ACATTGCACG GTCCGTGCAG ACCAGCCGTG AGCTAGCAGG CCTGCATCAC 
AGCCTGTCTC ACTCCCTCCT CACCGTGGCC CAGGCCCCTG AGGCCACTGT TCTGGAGGCT 
GAGACCCGAA AGCTGGATGA GTCTCTGAAT CAGAGTCTGA CATCCCCAGG GCCATGTTTG 
CTACATCCCA GTCTGGACAC TACTCAAAAT ACCCACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498865.1
CDS	95..4936
Translation	MSWGSGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	XM_006498802.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498802.3

ORF Insert Sequence:

ATGTCCTGGG GCTCAGGGGA AGTGAAATGC CTGAAGCTGG AACTGGATGC TGAGAGGACC 
CGGGCAGAGC AGGAGTGGGA TGCAGTAGCC AGGCAGCTAG CCCAAGCTGA GCAGGAGGGG 
CAAGCATCTC TGGAGCGACA GAAGGTGGCC CACGAGGAGG AGGTGAACAG GCTGCAAGAG 
AAATGGGAGA AAGAGCGCTC CTGGCTGCAG CAGGAGCTGG ATAAGACCCT GGAAACCCTC 
GAGAGGGAGA GAGCAGAGCT GGAGACAAAG CTGAGAGAGC AGCAGACAGA AATGGAAGCC 
ATCCGTGCAC AGAGGGAAGA GGAACGAAGC CAGGCTGACA GCGCCCTGTA TCAAATGCAG 
CTAGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGAGGAC CCAGAAGGAG 
CTGGCAGATG CTAGTCAACA GCTGGAAAGG CTGAGACAGG ACATGAAGAT TCAGAAGTTA 
AAGGAACAGG AGACCACTGG CATGCTGCAG GCCCAGCTTC AGGAAACACA GCAGGAGCTG 
AAGGAGGCAG CCCAGCAGCA CAGGGACGAC CTTGCTGCCT TCCAGAAGGA CAAGCTGGAC 
CTGCAGAAGC AGGTAGAGGA CCTAATGTCT CAGCTGGTAG CCCACGATGA CTCCCAGAGG 
CTGGTGAAAG AAGAGATTGA AGAGAAGGTG AAAGTGGCTC AAGAATGCAG CCGGATTCAG 
AAGGAACTGG AGAAAGAGAA CGCCAGCCTG GCTCTGTCGC TGGTGGAAAA GGAAAAACGA 
CTTCTCATTT TACAAGAAGC TGACTCTGTA AGACAGCAGG AGCTGAGCTC CTTGCGCCAG 
GACATACAGG AGGCCCAGGA AGGGCAAAGA GAGCTGGGTG TACAGGTGGA ACTATTGAGG 
CAGGAGGTGA AGGAAAAGGA GGCTGACTTT GTGGCCCGGG AAGCACAGCT GCTGGAGGAG 
TTGGAGGCCT CACGGGTTGC AGAGCAGCAA CTTCGAGCGT CTTTGTGGGC CCAGGAAGCC 
AAGGCAACCC AGCTGCAGCT GCAGCTGCGC AGCACTGAGA GCCAGCTAGA GGCATTGGTT 
GCAGAACAAC AGCCGGAGAA CCAGGCACAG GCACAGCTAG CTAGCCTCTG CTCTGTTCTG 
CAGCAGGCCC TGGGGTCTGC ATGTGAGAGT AGGCCTGAGC TGAGGGGTGG AGGCGACTCT 
GCTCCTACCT TATGGGGCCC TGATCCAGAT CAGAATGGAG CTTCCAGACT CTTTAAGAGA 
TGGTCGCTCC CAACGGCACT GTCACCGGAG GCTGTGGCAC TAGCTCTCCA GAAGCTCCAT 
CAGGACGTGT GGAAGGCTCG GCAGGCCCGG GATGACCTAA GGGACCAGGT CCAGAAGCTG 
GTGCAGCGCC TGACTGACAC GGAGGCCCAG AAGAGCCAGG TTCACTCGGA GCTGCAGGAC 
CTGCAGAGAC AGCTCTCTCA GAGCCAGGAA GAGAAATCCA AGTGGGAAGG GAGGCAGAAC 
TCCCTGGAGT CTGAGCTGAG GGACTTGCAC GAGACCGCGG CATCCTTACA GAGTCGCCTC 
CGGCAGGCAG AACTGCAAAA AATGGAAGCT CAGAATGATC GAGAGTTGCT TCAGGCATCC 
AAGGAGAAGC TGTCAGCCCA GGTTGAACAT CTGCAAGCAT GTGTTGCAGA AGCCCAGGCT 
CAGGCAGATG CAGCTGCTGT CCTGGAAGAA GATCTGCGAA CTGCTCGCTC TGCACTGAAA 
CTGAAAAACG AGGAGCTAGA GAGTGAACGG GAGAGAGCCC AGGCTTTACA AGAACAGGGT 
GAGCTGAAGG TGGCTCAGGG GAAAGCTTTG CAGGAGAACC TAGCCCTGCT GGCACAGACC 
CTGTCTAACA GAGAGCGGGA GGTCGAGACG TTGCAGGCTG AGGTTCAGGA ACTGGAGAAG 
CAGCGGGAGA TGCAGAAGGC TGCTCTGGAG CTGCTCTCCC TCGACCTGAA GAAGCGCAGT 
CGGGAGGTAG ACCTGCAACA AGAACAGATT CAGGAGCTGG AGCAGTGCAG GTCAGTCTTA 
GAGCACTTGC CCATGGCCGT CCAAGAGCGA GAGCAGAAAC TGAGTGTGCA GCGGGACCAG 
ATCAGGGAAC TGGAGAACGA TCGGGAGGCC CAGAGGAGTG TCCTGGAACA CCAGCTTCTG 
GACCTGGAGC AGAAGGCTCA AGTGATTGAG TCCCAGAGGG GACAGATTCA GGATCTGAAA 
AAGCAACTGG GGACACTGGA ATGCCTGGCC CTGGAACTGG AGGAAAGCCA TCACAAAGTG 
GAGAGCCAAC AGAAGATGAT CACAGAACTA GAGGGCCAGA GGGAAATGCA GAGGGTGGCT 
CTAACCCACC TCACACTGGA CCTGGAAGAA AGGAGCCAGG AGCTGCAGGC ACAAAGCAGC 
CAGCTCCATG AGCTGGAGAA CCATAGCACC CATCTGGCAA AAGAGCTGCA GGAGAGGGAC 
CAGGAGGTGA CGTCCCAACG CCAGCAGATC GATGAGCTGC AGAAGCAGCA GGAACAGCTG 
GCTCAGGCCC TGGAGAGGAA AGGCCAGGAG CTGGTGCTGC AGAAGGAAAG GATTCAGGTT 
CTGGAAGATC AGAGGACGCT GCAGACCAAG ATCTTAGAGG AGGACCTAGA ACAGATCAAG 
CATTCCTTGA GAGAACGCAG CCAGGAGCTG GCCTCTCAGT GGCAGCTGGT GCATGAGCGT 
GCCGACGATG GAAAGAGCCC GAGTAAAGGC CAGCGAGGGA GCCTGGAGCA CTTGAAGCTG 
ATCCTGCGTG ATAAGGAGAA GGAGGTAGAG TGCCAGCAGG AGCGCATCCA GGAACTTCAG 
GGCCACATGG GCCAGCTGGA ACAGCAGCTG CAGGGTCTGC ACAGGAAGGT GGGGGAGACT 
AGCCTGCTCC TGACCCATCG AGAGCAGGAG ACAGCAACCC TGCAGCAGCA TCTCCAGGAA 
GCCAAGGAAC AAGGGGAACT GAGAGAGCAG GTACTTCAGG GTCAGCTGGA GGAGGCCCAG 
AGAGATCTGG CCCAGAGGGA CCATGAACTA GAGACCCTCC GGCAGGAAAA ACAGCAGACC 
CAGGACCAGG AGGAAAGCAT GAAGCTAAAG ACAAGTGCTC TGCAGGCCGC TCTGGAGCAG 
GCCCATGCGA CACTGAAGGA GCGTCAGGGG GAGCTGGAAG AACACAGGGA ACAGGTGCGA 
AGGCTCCAGG AAGAACTAGA AGTGGAGGGA CGGCAGGTAC GGGCCCTGGA GGAGGTGTTG 
GGGGACCTAA GGGCTGAGTC TCGGGAGCAC GAGAAGGCTG TGCTGGCCCT TCAACAACGG 
TGTGCTGAGC AGGCCCAGGA ACATGAGGCA GAAGCCAGGA CCCTGCAGGA CAGCTGGCTG 
CAGGCCCAGG CCACTCTCAC AGAACAAGAA CAAGAGCTAG CGGCACTGCG AGCGGAAAAC 
CAGTATTCCC GCCGACAGGA AGAGGCTGCA GTTAGCCAGG CAGAGGCGCT GCAGGAGGCC 
CTCAGCAAAG CCCAGGCTGC CCTGCAGGAG AAAGAGCAGA GTCTGCTTGA ACAGGCTGAG 
TTGAGCCACA CCCTGGAGGC CAGCACTGCC GCCTTGCAAG CTACCCTGGA CACCTGCCAG 
GCAAGTGCCC GGCAGCTGGA GGAGGCCCTG AGGATTCGGG AAGGTGAGAT CCAGGCCCAG 
GCTCTCCAAC ACCACGAGGT CACGCAGCAC CTCCAGCAGG AACTTTGCCA GAAGAAAGAA 
GAGCTGAGGC AGCTACTAGA GAAGGCTGGG GCCCGGAGGA GTCAAGAAAA TGGAATCCAA 
GAGAAGCAAA GTCTGGAGCA AGAGAGACAA GAGGAAACAA GGAGGCTCCT TGAGAGCCTA 
AAGGAGCTAC AGCTGACTGT AGCCCAAAGG GAAGAAGAGA TTCTGATGCT GAGGGAGGCC 
TCGTCCCCAC GCCACAGAGC CCTCCCAGCC GAGAAGCCAG CTCTGCAACC GCTCCCAGCA 
CAACAGGAGT TAGAGCGGCT GCAGACTGCC CTAAGGCAAA CAGAGGCCAG GGAGATTGAA 
TGGAGGGAGA AGGCTCAGGA CCTGGCTCTG TCCCTGGCCC AAAGCAAGGC CAGCATCAGC 
AGTCTGCAGG AGATAACCAT GTTCCTACAA GCCTCTGTTC TAGAGCGGGA ATCAGAACAA 
CAAAGACTAC AGGAGGAGTT GGTACTCAGC AGACAAGCTC TGGAAGAGCA GCAGTCAGGT 
GGCCCACACT CAACCAGCAG AGCAGACCAG GGACCAAAAG TGGGACAGGG AAGCCAGTCT 
GGAGAGGTAG AGACTGAGCC TAGTCCTGGA GTGGAGGAGA AGGAACGGTT GACACAGAGG 
CTTGAACGTC TGCAGCAAGC GGTGGCAGAG CTGGAAGTTG ACCGAAGCAA GCTGCAGTGC 
CACAATGCGC AGCTGCGGAC CGCCTTGGAG CAGGTGGAAC GAGAACGGAG GAAGCTGAAG 
AGGGATTCCG TGCGTGCATC CCGGGCAGGA TCACTGGAGG CCAGAGAAAC TATGACTTCA 
TCCCCCACAC AGCAGGATGG GAGAGGCAGT CAGAGGGGTT CCTCGGACAG CGTGCTTGTG 
GTTGAGCTTC AGAGAGAGGT AGCCCTGCTG CGAGCCCAGC TGGCCTTGGA GCGAAAGCAG 
AGGCAGGACT ACATTGCACG GTCCGTGCAG ACCAGCCGTG AGCTAGCAGG CCTGCATCAC 
AGCCTGTCTC ACTCCCTCCT CACCGTGGCC CAGGCCCCTG AGGCCACTGT TCTGGAGGCT 
GAGACCCGAA AGCTGGATGA GTCTCTGAAT CAGAGTCTGA CATCCCCAGG GCCATGTTTG 
CTACATCCCA GTCTGGACAC TACTCAAAAT ACCCACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( XM_006498797.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498860.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu06037
Clone ID Related Accession (Same CDS sequence)	NM_001130000.1 , XM_006498796.1 , XM_006498797.3 , XM_006498797.4
Accession Version	XM_006498797.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498797.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGACCTTCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG GGAGCTGCTT CAGAAGGCCA GAGGAGAGCT GCAGCAGCAA 
CTGGAAGTGC TGGAGCAGGA AGCGTGGCGA CTGCGGAGGA TGAACATGGA GCTGCAGCTG 
CAGGGGGACT CAGCTCAAGG GGAGAAGCTG GAGCAGCAGG AGGAGTTGCA CTTGGCTGTC 
CGGGAGAGGG AGCGCCTTCA GGAGACGCTG GTGGGCCTGG AAGCCAAGCA GTCAGAATCA 
CTAAGTGAGC TGCTCACTCT TCGAGAAGCC CTGGAGTCAA GTCGCCTAGA AGGGGAGTTA 
CTGAAGCAGG AGCGAGTGGA AGTGGCTGCA GCCTTGGCCA GGGCAGAACA GTCCATTGTG 
GAGCTGTCAG GTTCTGAGAA CAGCCTGAAG GCAGAGGTAG CTGATCTTCG AGCTGCAGCT 
GTCAAGCTTG GGGCCTTAAA CGAGGCTTTG GCTTTAGATA AAGTTGAACT GAACCAGCAG 
CTTCTCCAGT TGGAGCAGGA GAACCAGTCT CTATGCAGCA GAGTGGAGGC CGCAGAGCAG 
CTGAGAAGCG CTTTGCAGGT GGACCTGGCG GAGGCAGAAA GGCGCAGGGA AGCCCTGTGG 
GAGAAGAAAA CTCAGCTGGA GACTCAGCTG CAGAAGGCAG AGGAAGCGGG GGCTGAGCTA 
CAGGCAGAGC TGAGGGGCAC CCGAGAAGAA AAGGAAGAGC TTAAAGATAA ATTAAGTGAG 
GCACATCACC AGCAAGAAAC AGCTACAGCT CACCTAGAAC AGCTGCATCA GGACGCAGAA 
AGACAGGAAG AAACACTTGC TAGGGCAGTC CAGGAGAAGG AAGCCCTAGT ACGGGAGAGG 
GCAGCTCTTG AGGTGCGCCT GCAGGCTGTG GAGCGGGACC GACAGGACCT CACTGAACAT 
GTCCTGGGGC TCAGGTCAGC CAAGGAGCAA CTGGAGAGCA ATCTATTTGA GGCCCAACAG 
CAAAATTCTG TGATACAGGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATTATC 
CAAGCCAAGG AAGTAATTCA AGGGGAAGTG AAATGCCTGA AGCTGGAACT GGATGCTGAG 
AGGACCCGGG CAGAGCAGGA GTGGGATGCA GTAGCCAGGC AGCTAGCCCA AGCTGAGCAG 
GAGGGGCAAG CATCTCTGGA GCGACAGAAG GTGGCCCACG AGGAGGAGGT GAACAGGCTG 
CAAGAGAAAT GGGAGAAAGA GCGCTCCTGG CTGCAGCAGG AGCTGGATAA GACCCTGGAA 
ACCCTCGAGA GGGAGAGAGC AGAGCTGGAG ACAAAGCTGA GAGAGCAGCA GACAGAAATG 
GAAGCCATCC GTGCACAGAG GGAAGAGGAA CGAAGCCAGG CTGACAGCGC CCTGTATCAA 
ATGCAGCTAG AAACAGAGAA GGAGAGAGTA TCCCTCCTGG AGACACTGCT GAGGACCCAG 
AAGGAGCTGG CAGATGCTAG TCAACAGCTG GAAAGGCTGA GACAGGACAT GAAGATTCAG 
AAGTTAAAGG AACAGGAGAC CACTGGCATG CTGCAGGCCC AGCTTCAGGA AACACAGCAG 
GAGCTGAAGG AGGCAGCCCA GCAGCACAGG GACGACCTTG CTGCCTTCCA GAAGGACAAG 
CTGGACCTGC AGAAGCAGGT AGAGGACCTA ATGTCTCAGC TGGTAGCCCA CGATGACTCC 
CAGAGGCTGG TGAAAGAAGA GATTGAAGAG AAGGTGAAAG TGGCTCAAGA ATGCAGCCGG 
ATTCAGAAGG AACTGGAGAA AGAGAACGCC AGCCTGGCTC TGTCGCTGGT GGAAAAGGAA 
AAACGACTTC TCATTTTACA AGAAGCTGAC TCTGTAAGAC AGCAGGAGCT GAGCTCCTTG 
CGCCAGGACA TACAGGAGGC CCAGGAAGGG CAAAGAGAGC TGGGTGTACA GGTGGAACTA 
TTGAGGCAGG AGGTGAAGGA AAAGGAGGCT GACTTTGTGG CCCGGGAAGC ACAGCTGCTG 
GAGGAGTTGG AGGCCTCACG GGTTGCAGAG CAGCAACTTC GAGCGTCTTT GTGGGCCCAG 
GAAGCCAAGG CAACCCAGCT GCAGCTGCAG CTGCGCAGCA CTGAGAGCCA GCTAGAGGCA 
TTGGTTGCAG AACAACAGCC GGAGAACCAG GCACAGGCAC AGCTAGCTAG CCTCTGCTCT 
GTTCTGCAGC AGGCCCTGGG GTCTGCATGT GAGAGTAGGC CTGAGCTGAG GGGTGGAGGC 
GACTCTGCTC CTACCTTATG GGGCCCTGAT CCAGATCAGA ATGGAGCTTC CAGACTCTTT 
AAGAGATGGT CGCTCCCAAC GGCACTGTCA CCGGAGGCTG TGGCACTAGC TCTCCAGAAG 
CTCCATCAGG ACGTGTGGAA GGCTCGGCAG GCCCGGGATG ACCTAAGGGA CCAGGTCCAG 
AAGCTGGTGC AGCGCCTGAC TGACACGGAG GCCCAGAAGA GCCAGGTTCA CTCGGAGCTG 
CAGGACCTGC AGAGACAGCT CTCTCAGAGC CAGGAAGAGA AATCCAAGTG GGAAGGGAGG 
CAGAACTCCC TGGAGTCTGA GCTGAGGGAC TTGCACGAGA CCGCGGCATC CTTACAGAGT 
CGCCTCCGGC AGGCAGAACT GCAAAAAATG GAAGCTCAGA ATGATCGAGA GTTGCTTCAG 
GCATCCAAGG AGAAGCTGTC AGCCCAGGTT GAACATCTGC AAGCATGTGT TGCAGAAGCC 
CAGGCTCAGG CAGATGCAGC TGCTGTCCTG GAAGAAGATC TGCGAACTGC TCGCTCTGCA 
CTGAAACTGA AAAACGAGGA GCTAGAGAGT GAACGGGAGA GAGCCCAGGC TTTACAAGAA 
CAGGGTGAGC TGAAGGTGGC TCAGGGGAAA GCTTTGCAGG AGAACCTAGC CCTGCTGGCA 
CAGACCCTGT CTAACAGAGA GCGGGAGGTC GAGACGTTGC AGGCTGAGGT TCAGGAACTG 
GAGAAGCAGC GGGAGATGCA GAAGGCTGCT CTGGAGCTGC TCTCCCTCGA CCTGAAGAAG 
CGCAGTCGGG AGGTAGACCT GCAACAAGAA CAGATTCAGG AGCTGGAGCA GTGCAGGTCA 
GTCTTAGAGC ACTTGCCCAT GGCCGTCCAA GAGCGAGAGC AGAAACTGAG TGTGCAGCGG 
GACCAGATCA GGGAACTGGA GAACGATCGG GAGGCCCAGA GGAGTGTCCT GGAACACCAG 
CTTCTGGACC TGGAGCAGAA GGCTCAAGTG ATTGAGTCCC AGAGGGGACA GATTCAGGAT 
CTGAAAAAGC AACTGGGGAC ACTGGAATGC CTGGCCCTGG AACTGGAGGA AAGCCATCAC 
AAAGTGGAGA GCCAACAGAA GATGATCACA GAACTAGAGG GCCAGAGGGA AATGCAGAGG 
GTGGCTCTAA CCCACCTCAC ACTGGACCTG GAAGAAAGGA GCCAGGAGCT GCAGGCACAA 
AGCAGCCAGC TCCATGAGCT GGAGAACCAT AGCACCCATC TGGCAAAAGA GCTGCAGGAG 
AGGGACCAGG AGGTGACGTC CCAACGCCAG CAGATCGATG AGCTGCAGAA GCAGCAGGAA 
CAGCTGGCTC AGGCCCTGGA GAGGAAAGGC CAGGAGCTGG TGCTGCAGAA GGAAAGGATT 
CAGGTTCTGG AAGATCAGAG GACGCTGCAG ACCAAGATCT TAGAGGAGGA CCTAGAACAG 
ATCAAGCATT CCTTGAGAGA ACGCAGCCAG GAGCTGGCCT CTCAGTGGCA GCTGGTGCAT 
GAGCGTGCCG ACGATGGAAA GAGCCCGAGT AAAGGCCAGC GAGGGAGCCT GGAGCACTTG 
AAGCTGATCC TGCGTGATAA GGAGAAGGAG GTAGAGTGCC AGCAGGAGCG CATCCAGGAA 
CTTCAGGGCC ACATGGGCCA GCTGGAACAG CAGCTGCAGG GTCTGCACAG GAAGGTGGGG 
GAGACTAGCC TGCTCCTGAC CCATCGAGAG CAGGAGACAG CAACCCTGCA GCAGCATCTC 
CAGGAAGCCA AGGAACAAGG GGAACTGAGA GAGCAGGTAC TTCAGGGTCA GCTGGAGGAG 
GCCCAGAGAG ATCTGGCCCA GAGGGACCAT GAACTAGAGA CCCTCCGGCA GGAAAAACAG 
CAGACCCAGG ACCAGGAGGA AAGCATGAAG CTAAAGACAA GTGCTCTGCA GGCCGCTCTG 
GAGCAGGCCC ATGCGACACT GAAGGAGCGT CAGGGGGAGC TGGAAGAACA CAGGGAACAG 
GTGCGAAGGC TCCAGGAAGA ACTAGAAGTG GAGGGACGGC AGGTACGGGC CCTGGAGGAG 
GTGTTGGGGG ACCTAAGGGC TGAGTCTCGG GAGCACGAGA AGGCTGTGCT GGCCCTTCAA 
CAACGGTGTG CTGAGCAGGC CCAGGAACAT GAGGCAGAAG CCAGGACCCT GCAGGACAGC 
TGGCTGCAGG CCCAGGCCAC TCTCACAGAA CAAGAACAAG AGCTAGCGGC ACTGCGAGCG 
GAAAACCAGT ATTCCCGCCG ACAGGAAGAG GCTGCAGTTA GCCAGGCAGA GGCGCTGCAG 
GAGGCCCTCA GCAAAGCCCA GGCTGCCCTG CAGGAGAAAG AGCAGAGTCT GCTTGAACAG 
GCTGAGTTGA GCCACACCCT GGAGGCCAGC ACTGCCGCCT TGCAAGCTAC CCTGGACACC 
TGCCAGGCAA GTGCCCGGCA GCTGGAGGAG GCCCTGAGGA TTCGGGAAGG TGAGATCCAG 
GCCCAGGCTC TCCAACACCA CGAGGTCACG CAGCACCTCC AGCAGGAACT TTGCCAGAAG 
AAAGAAGAGC TGAGGCAGCT ACTAGAGAAG GCTGGGGCCC GGAGGAGTCA AGAAAATGGA 
ATCCAAGAGA AGCAAAGTCT GGAGCAAGAG AGACAAGAGG AAACAAGGAG GCTCCTTGAG 
AGCCTAAAGG AGCTACAGCT GACTGTAGCC CAAAGGGAAG AAGAGATTCT GATGCTGAGG 
GAGGCCTCGT CCCCACGCCA CAGAGCCCTC CCAGCCGAGA AGCCAGCTCT GCAACCGCTC 
CCAGCACAAC AGGAGTTAGA GCGGCTGCAG ACTGCCCTAA GGCAAACAGA GGCCAGGGAG 
ATTGAATGGA GGGAGAAGGC TCAGGACCTG GCTCTGTCCC TGGCCCAAAG CAAGGCCAGC 
ATCAGCAGTC TGCAGGAGAT AACCATGTTC CTACAAGCCT CTGTTCTAGA GCGGGAATCA 
GAACAACAAA GACTACAGGA GGAGTTGGTA CTCAGCAGAC AAGCTCTGGA AGAGCAGCAG 
TCAGGTGGCC CACACTCAAC CAGCAGAGCA GACCAGGGAC CAAAAGTGGG ACAGGGAAGC 
CAGTCTGGAG AGGTAGAGAC TGAGCCTAGT CCTGGAGTGG AGGAGAAGGA ACGGTTGACA 
CAGAGGCTTG AACGTCTGCA GCAAGCGGTG GCAGAGCTGG AAGTTGACCG AAGCAAGCTG 
CAGTGCCACA ATGCGCAGCT GCGGACCGCC TTGGAGCAGG TGGAACGAGA ACGGAGGAAG 
CTGAAGAGGG ATTCCGTGCG TGCATCCCGG GCAGGATCAC TGGAGGCCAG AGAAACTATG 
ACTTCATCCC CCACACAGCA GGATGGGAGA GGCAGTCAGA GGGGTTCCTC GGACAGCGTG 
CTTGTGGTTG AGCTTCAGAG AGAGGTAGCC CTGCTGCGAG CCCAGCTGGC CTTGGAGCGA 
AAGCAGAGGC AGGACTACAT TGCACGGTCC GTGCAGACCA GCCGTGAGCT AGCAGGCCTG 
CATCACAGCC TGTCTCACTC CCTCCTCACC GTGGCCCAGG CCCCTGAGGC CACTGTTCTG 
GAGGCTGAGA CCCGAAAGCT GGATGAGTCT CTGAATCAGA GTCTGACATC CCCAGGGCCA 
TGTTTGCTAC ATCCCAGTCT GGACACTACT CAAAATACCC ACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498860.1
CDS	253..7560
Translation	METGSPGLNMKPQSLQLVLEGQVLALQQQMAENQAASWRKLKNSQEAQKRQATLVRKLQAKVLQYRSWCQDLEKRLEATGGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKLTVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	XM_006498797.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498797.4

ORF Insert Sequence:

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGACCTTCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG GGAGCTGCTT CAGAAGGCCA GAGGAGAGCT GCAGCAGCAA 
CTGGAAGTGC TGGAGCAGGA AGCGTGGCGA CTGCGGAGGA TGAACATGGA GCTGCAGCTG 
CAGGGGGACT CAGCTCAAGG GGAGAAGCTG GAGCAGCAGG AGGAGTTGCA CTTGGCTGTC 
CGGGAGAGGG AGCGCCTTCA GGAGACGCTG GTGGGCCTGG AAGCCAAGCA GTCAGAATCA 
CTAAGTGAGC TGCTCACTCT TCGAGAAGCC CTGGAGTCAA GTCGCCTAGA AGGGGAGTTA 
CTGAAGCAGG AGCGAGTGGA AGTGGCTGCA GCCTTGGCCA GGGCAGAACA GTCCATTGTG 
GAGCTGTCAG GTTCTGAGAA CAGCCTGAAG GCAGAGGTAG CTGATCTTCG AGCTGCAGCT 
GTCAAGCTTG GGGCCTTAAA CGAGGCTTTG GCTTTAGATA AAGTTGAACT GAACCAGCAG 
CTTCTCCAGT TGGAGCAGGA GAACCAGTCT CTATGCAGCA GAGTGGAGGC CGCAGAGCAG 
CTGAGAAGCG CTTTGCAGGT GGACCTGGCG GAGGCAGAAA GGCGCAGGGA AGCCCTGTGG 
GAGAAGAAAA CTCAGCTGGA GACTCAGCTG CAGAAGGCAG AGGAAGCGGG GGCTGAGCTA 
CAGGCAGAGC TGAGGGGCAC CCGAGAAGAA AAGGAAGAGC TTAAAGATAA ATTAAGTGAG 
GCACATCACC AGCAAGAAAC AGCTACAGCT CACCTAGAAC AGCTGCATCA GGACGCAGAA 
AGACAGGAAG AAACACTTGC TAGGGCAGTC CAGGAGAAGG AAGCCCTAGT ACGGGAGAGG 
GCAGCTCTTG AGGTGCGCCT GCAGGCTGTG GAGCGGGACC GACAGGACCT CACTGAACAT 
GTCCTGGGGC TCAGGTCAGC CAAGGAGCAA CTGGAGAGCA ATCTATTTGA GGCCCAACAG 
CAAAATTCTG TGATACAGGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATTATC 
CAAGCCAAGG AAGTAATTCA AGGGGAAGTG AAATGCCTGA AGCTGGAACT GGATGCTGAG 
AGGACCCGGG CAGAGCAGGA GTGGGATGCA GTAGCCAGGC AGCTAGCCCA AGCTGAGCAG 
GAGGGGCAAG CATCTCTGGA GCGACAGAAG GTGGCCCACG AGGAGGAGGT GAACAGGCTG 
CAAGAGAAAT GGGAGAAAGA GCGCTCCTGG CTGCAGCAGG AGCTGGATAA GACCCTGGAA 
ACCCTCGAGA GGGAGAGAGC AGAGCTGGAG ACAAAGCTGA GAGAGCAGCA GACAGAAATG 
GAAGCCATCC GTGCACAGAG GGAAGAGGAA CGAAGCCAGG CTGACAGCGC CCTGTATCAA 
ATGCAGCTAG AAACAGAGAA GGAGAGAGTA TCCCTCCTGG AGACACTGCT GAGGACCCAG 
AAGGAGCTGG CAGATGCTAG TCAACAGCTG GAAAGGCTGA GACAGGACAT GAAGATTCAG 
AAGTTAAAGG AACAGGAGAC CACTGGCATG CTGCAGGCCC AGCTTCAGGA AACACAGCAG 
GAGCTGAAGG AGGCAGCCCA GCAGCACAGG GACGACCTTG CTGCCTTCCA GAAGGACAAG 
CTGGACCTGC AGAAGCAGGT AGAGGACCTA ATGTCTCAGC TGGTAGCCCA CGATGACTCC 
CAGAGGCTGG TGAAAGAAGA GATTGAAGAG AAGGTGAAAG TGGCTCAAGA ATGCAGCCGG 
ATTCAGAAGG AACTGGAGAA AGAGAACGCC AGCCTGGCTC TGTCGCTGGT GGAAAAGGAA 
AAACGACTTC TCATTTTACA AGAAGCTGAC TCTGTAAGAC AGCAGGAGCT GAGCTCCTTG 
CGCCAGGACA TACAGGAGGC CCAGGAAGGG CAAAGAGAGC TGGGTGTACA GGTGGAACTA 
TTGAGGCAGG AGGTGAAGGA AAAGGAGGCT GACTTTGTGG CCCGGGAAGC ACAGCTGCTG 
GAGGAGTTGG AGGCCTCACG GGTTGCAGAG CAGCAACTTC GAGCGTCTTT GTGGGCCCAG 
GAAGCCAAGG CAACCCAGCT GCAGCTGCAG CTGCGCAGCA CTGAGAGCCA GCTAGAGGCA 
TTGGTTGCAG AACAACAGCC GGAGAACCAG GCACAGGCAC AGCTAGCTAG CCTCTGCTCT 
GTTCTGCAGC AGGCCCTGGG GTCTGCATGT GAGAGTAGGC CTGAGCTGAG GGGTGGAGGC 
GACTCTGCTC CTACCTTATG GGGCCCTGAT CCAGATCAGA ATGGAGCTTC CAGACTCTTT 
AAGAGATGGT CGCTCCCAAC GGCACTGTCA CCGGAGGCTG TGGCACTAGC TCTCCAGAAG 
CTCCATCAGG ACGTGTGGAA GGCTCGGCAG GCCCGGGATG ACCTAAGGGA CCAGGTCCAG 
AAGCTGGTGC AGCGCCTGAC TGACACGGAG GCCCAGAAGA GCCAGGTTCA CTCGGAGCTG 
CAGGACCTGC AGAGACAGCT CTCTCAGAGC CAGGAAGAGA AATCCAAGTG GGAAGGGAGG 
CAGAACTCCC TGGAGTCTGA GCTGAGGGAC TTGCACGAGA CCGCGGCATC CTTACAGAGT 
CGCCTCCGGC AGGCAGAACT GCAAAAAATG GAAGCTCAGA ATGATCGAGA GTTGCTTCAG 
GCATCCAAGG AGAAGCTGTC AGCCCAGGTT GAACATCTGC AAGCATGTGT TGCAGAAGCC 
CAGGCTCAGG CAGATGCAGC TGCTGTCCTG GAAGAAGATC TGCGAACTGC TCGCTCTGCA 
CTGAAACTGA AAAACGAGGA GCTAGAGAGT GAACGGGAGA GAGCCCAGGC TTTACAAGAA 
CAGGGTGAGC TGAAGGTGGC TCAGGGGAAA GCTTTGCAGG AGAACCTAGC CCTGCTGGCA 
CAGACCCTGT CTAACAGAGA GCGGGAGGTC GAGACGTTGC AGGCTGAGGT TCAGGAACTG 
GAGAAGCAGC GGGAGATGCA GAAGGCTGCT CTGGAGCTGC TCTCCCTCGA CCTGAAGAAG 
CGCAGTCGGG AGGTAGACCT GCAACAAGAA CAGATTCAGG AGCTGGAGCA GTGCAGGTCA 
GTCTTAGAGC ACTTGCCCAT GGCCGTCCAA GAGCGAGAGC AGAAACTGAG TGTGCAGCGG 
GACCAGATCA GGGAACTGGA GAACGATCGG GAGGCCCAGA GGAGTGTCCT GGAACACCAG 
CTTCTGGACC TGGAGCAGAA GGCTCAAGTG ATTGAGTCCC AGAGGGGACA GATTCAGGAT 
CTGAAAAAGC AACTGGGGAC ACTGGAATGC CTGGCCCTGG AACTGGAGGA AAGCCATCAC 
AAAGTGGAGA GCCAACAGAA GATGATCACA GAACTAGAGG GCCAGAGGGA AATGCAGAGG 
GTGGCTCTAA CCCACCTCAC ACTGGACCTG GAAGAAAGGA GCCAGGAGCT GCAGGCACAA 
AGCAGCCAGC TCCATGAGCT GGAGAACCAT AGCACCCATC TGGCAAAAGA GCTGCAGGAG 
AGGGACCAGG AGGTGACGTC CCAACGCCAG CAGATCGATG AGCTGCAGAA GCAGCAGGAA 
CAGCTGGCTC AGGCCCTGGA GAGGAAAGGC CAGGAGCTGG TGCTGCAGAA GGAAAGGATT 
CAGGTTCTGG AAGATCAGAG GACGCTGCAG ACCAAGATCT TAGAGGAGGA CCTAGAACAG 
ATCAAGCATT CCTTGAGAGA ACGCAGCCAG GAGCTGGCCT CTCAGTGGCA GCTGGTGCAT 
GAGCGTGCCG ACGATGGAAA GAGCCCGAGT AAAGGCCAGC GAGGGAGCCT GGAGCACTTG 
AAGCTGATCC TGCGTGATAA GGAGAAGGAG GTAGAGTGCC AGCAGGAGCG CATCCAGGAA 
CTTCAGGGCC ACATGGGCCA GCTGGAACAG CAGCTGCAGG GTCTGCACAG GAAGGTGGGG 
GAGACTAGCC TGCTCCTGAC CCATCGAGAG CAGGAGACAG CAACCCTGCA GCAGCATCTC 
CAGGAAGCCA AGGAACAAGG GGAACTGAGA GAGCAGGTAC TTCAGGGTCA GCTGGAGGAG 
GCCCAGAGAG ATCTGGCCCA GAGGGACCAT GAACTAGAGA CCCTCCGGCA GGAAAAACAG 
CAGACCCAGG ACCAGGAGGA AAGCATGAAG CTAAAGACAA GTGCTCTGCA GGCCGCTCTG 
GAGCAGGCCC ATGCGACACT GAAGGAGCGT CAGGGGGAGC TGGAAGAACA CAGGGAACAG 
GTGCGAAGGC TCCAGGAAGA ACTAGAAGTG GAGGGACGGC AGGTACGGGC CCTGGAGGAG 
GTGTTGGGGG ACCTAAGGGC TGAGTCTCGG GAGCACGAGA AGGCTGTGCT GGCCCTTCAA 
CAACGGTGTG CTGAGCAGGC CCAGGAACAT GAGGCAGAAG CCAGGACCCT GCAGGACAGC 
TGGCTGCAGG CCCAGGCCAC TCTCACAGAA CAAGAACAAG AGCTAGCGGC ACTGCGAGCG 
GAAAACCAGT ATTCCCGCCG ACAGGAAGAG GCTGCAGTTA GCCAGGCAGA GGCGCTGCAG 
GAGGCCCTCA GCAAAGCCCA GGCTGCCCTG CAGGAGAAAG AGCAGAGTCT GCTTGAACAG 
GCTGAGTTGA GCCACACCCT GGAGGCCAGC ACTGCCGCCT TGCAAGCTAC CCTGGACACC 
TGCCAGGCAA GTGCCCGGCA GCTGGAGGAG GCCCTGAGGA TTCGGGAAGG TGAGATCCAG 
GCCCAGGCTC TCCAACACCA CGAGGTCACG CAGCACCTCC AGCAGGAACT TTGCCAGAAG 
AAAGAAGAGC TGAGGCAGCT ACTAGAGAAG GCTGGGGCCC GGAGGAGTCA AGAAAATGGA 
ATCCAAGAGA AGCAAAGTCT GGAGCAAGAG AGACAAGAGG AAACAAGGAG GCTCCTTGAG 
AGCCTAAAGG AGCTACAGCT GACTGTAGCC CAAAGGGAAG AAGAGATTCT GATGCTGAGG 
GAGGCCTCGT CCCCACGCCA CAGAGCCCTC CCAGCCGAGA AGCCAGCTCT GCAACCGCTC 
CCAGCACAAC AGGAGTTAGA GCGGCTGCAG ACTGCCCTAA GGCAAACAGA GGCCAGGGAG 
ATTGAATGGA GGGAGAAGGC TCAGGACCTG GCTCTGTCCC TGGCCCAAAG CAAGGCCAGC 
ATCAGCAGTC TGCAGGAGAT AACCATGTTC CTACAAGCCT CTGTTCTAGA GCGGGAATCA 
GAACAACAAA GACTACAGGA GGAGTTGGTA CTCAGCAGAC AAGCTCTGGA AGAGCAGCAG 
TCAGGTGGCC CACACTCAAC CAGCAGAGCA GACCAGGGAC CAAAAGTGGG ACAGGGAAGC 
CAGTCTGGAG AGGTAGAGAC TGAGCCTAGT CCTGGAGTGG AGGAGAAGGA ACGGTTGACA 
CAGAGGCTTG AACGTCTGCA GCAAGCGGTG GCAGAGCTGG AAGTTGACCG AAGCAAGCTG 
CAGTGCCACA ATGCGCAGCT GCGGACCGCC TTGGAGCAGG TGGAACGAGA ACGGAGGAAG 
CTGAAGAGGG ATTCCGTGCG TGCATCCCGG GCAGGATCAC TGGAGGCCAG AGAAACTATG 
ACTTCATCCC CCACACAGCA GGATGGGAGA GGCAGTCAGA GGGGTTCCTC GGACAGCGTG 
CTTGTGGTTG AGCTTCAGAG AGAGGTAGCC CTGCTGCGAG CCCAGCTGGC CTTGGAGCGA 
AAGCAGAGGC AGGACTACAT TGCACGGTCC GTGCAGACCA GCCGTGAGCT AGCAGGCCTG 
CATCACAGCC TGTCTCACTC CCTCCTCACC GTGGCCCAGG CCCCTGAGGC CACTGTTCTG 
GAGGCTGAGA CCCGAAAGCT GGATGAGTCT CTGAATCAGA GTCTGACATC CCCAGGGCCA 
TGTTTGCTAC ATCCCAGTCT GGACACTACT CAAAATACCC ACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( XM_006498799.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498862.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant X4, mRNA.

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CloneID	OMu30043
Clone ID Related Accession (Same CDS sequence)	XM_006498799.2 , XM_006498799.3
Accession Version	XM_006498799.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498799.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTCC GTGGATGCAG TATGTGGATG GAATTAGAGA GAAAATTCAT GTTCATCCAG 
CAGGGGCTGA TCCCTCAGCG CTGGGAGAGT GTAGAAGAGC CCAACCTGGA ACAGTTGCTG 
ATCCGATTGG AGGAGGAGCA GCAGAGGTGT GAGAGTCTAG TGGAGGTGAA CACCGAGCTT 
CGGCTGCACA TGGAGAAAGC CGATGTGGTG AATAAAGCCT TGCAGGAGGA CGTGGAAAAA 
CTGACAGTGG ACTGGAGTCG GGCCCGAGAT GAGTTAGTGA GGAAGGAGAG CCAGTGGCGG 
ATGGAGCAGG AGTTCTTCAA GGGCTATTTG AGAGGTGAAC ACGGTCGTCT TCTCAATCTA 
TGGCGAGAGG TGGTAACCTT CCGACGTCAC TTCCTGAAAA TGAAGTCAGC TACTGACAGA 
GACCTGACAG AGCTAAAGGC TGAACACGCA AGGCTTTCAG GGTCCCTGCT GACCTGCTGT 
CTGCGCCTGA CCCTGAGAGC ACAGTCTCGG GAGTCCAGTG GATCTGGGAG AACAGAGGAG 
AGTGAGCCAG CCCGGCTGCT GCTGCTCGTC GCTAAGACCC AGGCGCTGGA GAAGGAAGCC 
CATGAGAAGA GCCAGGAGTT AATGCAGCTC AAGAGTCACG GGGATCTAGA GAAGGCAGAG 
CTGCAGGACC GAGTGACGGA ACTCTCTGCT CTGCTAACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAGA AGATGGTAAA GGCTCTAAGA GAAACAATGG AGATCCTGGA GACAAACCAT 
GCAGAGTTAA TGGAGCATGA GGCATCTCTC AGTAGGAATG CTCAAGAGGA AAAGTTGTCT 
CTGCAGCAGG TGATCAAGGC TATAACCCAG GCACTGGCCT CAGTGGAAGA AGAAGACACT 
GTGACCCAGT CCTCAGGCCA TGAGGACTCG CTGCAGTCAG ACTGTAATGG CCTTTCCCAG 
TTTGATCCCC AGGACCCAGA CAGGGCTCTT ACCCTGGTGC AGTCAGTGCT GACTCGGAGA 
CAGCAGGCTG TGCAGGACCT CAGGCAGCAG CTTTCAGGCT GCCAGGAGGC TATGAGCTTC 
TTGCAGCAGC AGCACGATCA GTGGGAGGAA GAGGGCAGGG CCCTGAGGGA AAAGCTACAG 
AAGCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGGCCTTCA GGGAGAGGTG 
GACTCTCTCA CTGGGGAGCG GGATGCTCTG GCAGGCCAGA CTGTGGACCT TCAGGGAGAG 
GTGGACTCTC TCAGCAGGGA GCGGGAGCTG CTTCAGAAGG CCAGAGGAGA GCTGCAGCAG 
CAACTGGAAG TGCTGGAGCA GGAAGCGTGG CGACTGCGGA GGATGAACAT GGAGCTGCAG 
CTGCAGGGGG ACTCAGCTCA AGGGGAGAAG CTGGAGCAGC AGGAGGAGTT GCACTTGGCT 
GTCCGGGAGA GGGAGCGCCT TCAGGAGACG CTGGTGGGCC TGGAAGCCAA GCAGTCAGAA 
TCACTAAGTG AGCTGCTCAC TCTTCGAGAA GCCCTGGAGT CAAGTCGCCT AGAAGGGGAG 
TTACTGAAGC AGGAGCGAGT GGAAGTGGCT GCAGCCTTGG CCAGGGCAGA ACAGTCCATT 
GTGGAGCTGT CAGGTTCTGA GAACAGCCTG AAGGCAGAGG TAGCTGATCT TCGAGCTGCA 
GCTGTCAAGC TTGGGGCCTT AAACGAGGCT TTGGCTTTAG ATAAAGTTGA ACTGAACCAG 
CAGCTTCTCC AGTTGGAGCA GGAGAACCAG TCTCTATGCA GCAGAGTGGA GGCCGCAGAG 
CAGCTGAGAA GCGCTTTGCA GGTGGACCTG GCGGAGGCAG AAAGGCGCAG GGAAGCCCTG 
TGGGAGAAGA AAACTCAGCT GGAGACTCAG CTGCAGAAGG CAGAGGAAGC GGGGGCTGAG 
CTACAGGCAG AGCTGAGGGG CACCCGAGAA GAAAAGGAAG AGCTTAAAGA TAAATTAAGT 
GAGGCACATC ACCAGCAAGA AACAGCTACA GCTCACCTAG AACAGCTGCA TCAGGACGCA 
GAAAGACAGG AAGAAACACT TGCTAGGGCA GTCCAGGAGA AGGAAGCCCT AGTACGGGAG 
AGGGCAGCTC TTGAGGTGCG CCTGCAGGCT GTGGAGCGGG ACCGACAGGA CCTCACTGAA 
CATGTCCTGG GGCTCAGGTC AGCCAAGGAG CAACTGGAGA GCAATCTATT TGAGGCCCAA 
CAGCAAAATT CTGTGATACA GGTCACCAAG GGGCAGCTGG AGGTCCAGAT TCAAACTATT 
ATCCAAGCCA AGGAAGTAAT TCAAGGGGAA GTGAAATGCC TGAAGCTGGA ACTGGATGCT 
GAGAGGACCC GGGCAGAGCA GGAGTGGGAT GCAGTAGCCA GGCAGCTAGC CCAAGCTGAG 
CAGGAGGGGC AAGCATCTCT GGAGCGACAG AAGGTGGCCC ACGAGGAGGA GGTGAACAGG 
CTGCAAGAGA AATGGGAGAA AGAGCGCTCC TGGCTGCAGC AGGAGCTGGA TAAGACCCTG 
GAAACCCTCG AGAGGGAGAG AGCAGAGCTG GAGACAAAGC TGAGAGAGCA GCAGACAGAA 
ATGGAAGCCA TCCGTGCACA GAGGGAAGAG GAACGAAGCC AGGCTGACAG CGCCCTGTAT 
CAAATGCAGC TAGAAACAGA GAAGGAGAGA GTATCCCTCC TGGAGACACT GCTGAGGACC 
CAGAAGGAGC TGGCAGATGC TAGTCAACAG CTGGAAAGGC TGAGACAGGA CATGAAGATT 
CAGAAGTTAA AGGAACAGGA GACCACTGGC ATGCTGCAGG CCCAGCTTCA GGAAACACAG 
CAGGAGCTGA AGGAGGCAGC CCAGCAGCAC AGGGACGACC TTGCTGCCTT CCAGAAGGAC 
AAGCTGGACC TGCAGAAGCA GGTAGAGGAC CTAATGTCTC AGCTGGTAGC CCACGATGAC 
TCCCAGAGGC TGGTGAAAGA AGAGATTGAA GAGAAGGTGA AAGTGGCTCA AGAATGCAGC 
CGGATTCAGA AGGAACTGGA GAAAGAGAAC GCCAGCCTGG CTCTGTCGCT GGTGGAAAAG 
GAAAAACGAC TTCTCATTTT ACAAGAAGCT GACTCTGTAA GACAGCAGGA GCTGAGCTCC 
TTGCGCCAGG ACATACAGGA GGCCCAGGAA GGGCAAAGAG AGCTGGGTGT ACAGGTGGAA 
CTATTGAGGC AGGAGGTGAA GGAAAAGGAG GCTGACTTTG TGGCCCGGGA AGCACAGCTG 
CTGGAGGAGT TGGAGGCCTC ACGGGTTGCA GAGCAGCAAC TTCGAGCGTC TTTGTGGGCC 
CAGGAAGCCA AGGCAACCCA GCTGCAGCTG CAGCTGCGCA GCACTGAGAG CCAGCTAGAG 
GCATTGGTTG CAGAACAACA GCCGGAGAAC CAGGCACAGG CACAGCTAGC TAGCCTCTGC 
TCTGTTCTGC AGCAGGCCCT GGGGTCTGCA TGTGAGAGTA GGCCTGAGCT GAGGGGTGGA 
GGCGACTCTG CTCCTACCTT ATGGGGCCCT GATCCAGATC AGAATGGAGC TTCCAGACTC 
TTTAAGAGAT GGTCGCTCCC AACGGCACTG TCACCGGAGG CTGTGGCACT AGCTCTCCAG 
AAGCTCCATC AGGACGTGTG GAAGGCTCGG CAGGCCCGGG ATGACCTAAG GGACCAGGTC 
CAGAAGCTGG TGCAGCGCCT GACTGACACG GAGGCCCAGA AGAGCCAGGT TCACTCGGAG 
CTGCAGGACC TGCAGAGACA GCTCTCTCAG AGCCAGGAAG AGAAATCCAA GTGGGAAGGG 
AGGCAGAACT CCCTGGAGTC TGAGCTGAGG GACTTGCACG AGACCGCGGC ATCCTTACAG 
AGTCGCCTCC GGCAGGCAGA ACTGCAAAAA ATGGAAGCTC AGAATGATCG AGAGTTGCTT 
CAGGCATCCA AGGAGAAGCT GTCAGCCCAG GTTGAACATC TGCAAGCATG TGTTGCAGAA 
GCCCAGGCTC AGGCAGATGC AGCTGCTGTC CTGGAAGAAG ATCTGCGAAC TGCTCGCTCT 
GCACTGAAAC TGAAAAACGA GGAGCTAGAG AGTGAACGGG AGAGAGCCCA GGCTTTACAA 
GAACAGGGTG AGCTGAAGGT GGCTCAGGGG AAAGCTTTGC AGGAGAACCT AGCCCTGCTG 
GCACAGACCC TGTCTAACAG AGAGCGGGAG GTCGAGACGT TGCAGGCTGA GGTTCAGGAA 
CTGGAGAAGC AGCGGGAGAT GCAGAAGGCT GCTCTGGAGC TGCTCTCCCT CGACCTGAAG 
AAGCGCAGTC GGGAGGTAGA CCTGCAACAA GAACAGATTC AGGAGCTGGA GCAGTGCAGG 
TCAGTCTTAG AGCACTTGCC CATGGCCGTC CAAGAGCGAG AGCAGAAACT GAGTGTGCAG 
CGGGACCAGA TCAGGGAACT GGAGAACGAT CGGGAGGCCC AGAGGAGTGT CCTGGAACAC 
CAGCTTCTGG ACCTGGAGCA GAAGGCTCAA GTGATTGAGT CCCAGAGGGG ACAGATTCAG 
GATCTGAAAA AGCAACTGGG GACACTGGAA TGCCTGGCCC TGGAACTGGA GGAAAGCCAT 
CACAAAGTGG AGAGCCAACA GAAGATGATC ACAGAACTAG AGGGCCAGAG GGAAATGCAG 
AGGGTGGCTC TAACCCACCT CACACTGGAC CTGGAAGAAA GGAGCCAGGA GCTGCAGGCA 
CAAAGCAGCC AGCTCCATGA GCTGGAGAAC CATAGCACCC ATCTGGCAAA AGAGCTGCAG 
GAGAGGGACC AGGAGGTGAC GTCCCAACGC CAGCAGATCG ATGAGCTGCA GAAGCAGCAG 
GAACAGCTGG CTCAGGCCCT GGAGAGGAAA GGCCAGGAGC TGGTGCTGCA GAAGGAAAGG 
ATTCAGGTTC TGGAAGATCA GAGGACGCTG CAGACCAAGA TCTTAGAGGA GGACCTAGAA 
CAGATCAAGC ATTCCTTGAG AGAACGCAGC CAGGAGCTGG CCTCTCAGTG GCAGCTGGTG 
CATGAGCGTG CCGACGATGG AAAGAGCCCG AGTAAAGGCC AGCGAGGGAG CCTGGAGCAC 
TTGAAGCTGA TCCTGCGTGA TAAGGAGAAG GAGGTAGAGT GCCAGCAGGA GCGCATCCAG 
GAACTTCAGG GCCACATGGG CCAGCTGGAA CAGCAGCTGC AGGGTCTGCA CAGGAAGGTG 
GGGGAGACTA GCCTGCTCCT GACCCATCGA GAGCAGGAGA CAGCAACCCT GCAGCAGCAT 
CTCCAGGAAG CCAAGGAACA AGGGGAACTG AGAGAGCAGG TACTTCAGGG TCAGCTGGAG 
GAGGCCCAGA GAGATCTGGC CCAGAGGGAC CATGAACTAG AGACCCTCCG GCAGGAAAAA 
CAGCAGACCC AGGACCAGGA GGAAAGCATG AAGCTAAAGA CAAGTGCTCT GCAGGCCGCT 
CTGGAGCAGG CCCATGCGAC ACTGAAGGAG CGTCAGGGGG AGCTGGAAGA ACACAGGGAA 
CAGGTGCGAA GGCTCCAGGA AGAACTAGAA GTGGAGGGAC GGCAGGTACG GGCCCTGGAG 
GAGGTGTTGG GGGACCTAAG GGCTGAGTCT CGGGAGCACG AGAAGGCTGT GCTGGCCCTT 
CAACAACGGT GTGCTGAGCA GGCCCAGGAA CATGAGGCAG AAGCCAGGAC CCTGCAGGAC 
AGCTGGCTGC AGGCCCAGGC CACTCTCACA GAACAAGAAC AAGAGCTAGC GGCACTGCGA 
GCGGAAAACC AGTATTCCCG CCGACAGGAA GAGGCTGCAG TTAGCCAGGC AGAGGCGCTG 
CAGGAGGCCC TCAGCAAAGC CCAGGCTGCC CTGCAGGAGA AAGAGCAGAG TCTGCTTGAA 
CAGGCTGAGT TGAGCCACAC CCTGGAGGCC AGCACTGCCG CCTTGCAAGC TACCCTGGAC 
ACCTGCCAGG CAAGTGCCCG GCAGCTGGAG GAGGCCCTGA GGATTCGGGA AGGTGAGATC 
CAGGCCCAGG CTCTCCAACA CCACGAGGTC ACGCAGCACC TCCAGCAGGA ACTTTGCCAG 
AAGAAAGAAG AGCTGAGGCA GCTACTAGAG AAGGCTGGGG CCCGGAGGAG TCAAGAAAAT 
GGAATCCAAG AGAAGCAAAG TCTGGAGCAA GAGAGACAAG AGGAAACAAG GAGGCTCCTT 
GAGAGCCTAA AGGAGCTACA GCTGACTGTA GCCCAAAGGG AAGAAGAGAT TCTGATGCTG 
AGGGAGGCCT CGTCCCCACG CCACAGAGCC CTCCCAGCCG AGAAGCCAGC TCTGCAACCG 
CTCCCAGCAC AACAGGAGTT AGAGCGGCTG CAGACTGCCC TAAGGCAAAC AGAGGCCAGG 
GAGATTGAAT GGAGGGAGAA GGCTCAGGAC CTGGCTCTGT CCCTGGCCCA AAGCAAGGCC 
AGCATCAGCA GTCTGCAGGA GATAACCATG TTCCTACAAG CCTCTGTTCT AGAGCGGGAA 
TCAGAACAAC AAAGACTACA GGAGGAGTTG GTACTCAGCA GACAAGCTCT GGAAGAGCAG 
CAGTCAGGTG GCCCACACTC AACCAGCAGA GCAGACCAGG GACCAAAAGT GGGACAGGGA 
AGCCAGTCTG GAGAGGTAGA GACTGAGCCT AGTCCTGGAG TGGAGGAGAA GGAACGGTTG 
ACACAGAGGC TTGAACGTCT GCAGCAAGCG GTGGCAGAGC TGGAAGTTGA CCGAAGCAAG 
CTGCAGTGCC ACAATGCGCA GCTGCGGACC GCCTTGGAGC AGGTGGAACG AGAACGGAGG 
AAGCTGAAGA GGGATTCCGT GCGTGCATCC CGGGCAGGAT CACTGGAGGC CAGAGAAACT 
ATGACTTCAT CCCCCACACA GCAGGATGGG AGAGGCAGTC AGAGGGGTTC CTCGGACAGC 
GTGCTTGTGG TTGAGCTTCA GAGAGAGGTA GCCCTGCTGC GAGCCCAGCT GGCCTTGGAG 
CGAAAGCAGA GGCAGGACTA CATTGCACGG TCCGTGCAGA CCAGCCGTGA GCTAGCAGGC 
CTGCATCACA GCCTGTCTCA CTCCCTCCTC ACCGTGGCCC AGGCCCCTGA GGCCACTGTT 
CTGGAGGCTG AGACCCGAAA GCTGGATGAG TCTCTGAATC AGAGTCTGAC ATCCCCAGGG 
CCATGTTTGC TACATCCCAG TCTGGACACT ACTCAAAATA CCCACAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498862.1
CDS	76..7206
Translation	MAFRGCSMWMELERKFMFIQQGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKLTVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	XM_006498799.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498799.3

ORF Insert Sequence:

ATGGCTTTCC GTGGATGCAG TATGTGGATG GAATTAGAGA GAAAATTCAT GTTCATCCAG 
CAGGGGCTGA TCCCTCAGCG CTGGGAGAGT GTAGAAGAGC CCAACCTGGA ACAGTTGCTG 
ATCCGATTGG AGGAGGAGCA GCAGAGGTGT GAGAGTCTAG TGGAGGTGAA CACCGAGCTT 
CGGCTGCACA TGGAGAAAGC CGATGTGGTG AATAAAGCCT TGCAGGAGGA CGTGGAAAAA 
CTGACAGTGG ACTGGAGTCG GGCCCGAGAT GAGTTAGTGA GGAAGGAGAG CCAGTGGCGG 
ATGGAGCAGG AGTTCTTCAA GGGCTATTTG AGAGGTGAAC ACGGTCGTCT TCTCAATCTA 
TGGCGAGAGG TGGTAACCTT CCGACGTCAC TTCCTGAAAA TGAAGTCAGC TACTGACAGA 
GACCTGACAG AGCTAAAGGC TGAACACGCA AGGCTTTCAG GGTCCCTGCT GACCTGCTGT 
CTGCGCCTGA CCCTGAGAGC ACAGTCTCGG GAGTCCAGTG GATCTGGGAG AACAGAGGAG 
AGTGAGCCAG CCCGGCTGCT GCTGCTCGTC GCTAAGACCC AGGCGCTGGA GAAGGAAGCC 
CATGAGAAGA GCCAGGAGTT AATGCAGCTC AAGAGTCACG GGGATCTAGA GAAGGCAGAG 
CTGCAGGACC GAGTGACGGA ACTCTCTGCT CTGCTAACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAGA AGATGGTAAA GGCTCTAAGA GAAACAATGG AGATCCTGGA GACAAACCAT 
GCAGAGTTAA TGGAGCATGA GGCATCTCTC AGTAGGAATG CTCAAGAGGA AAAGTTGTCT 
CTGCAGCAGG TGATCAAGGC TATAACCCAG GCACTGGCCT CAGTGGAAGA AGAAGACACT 
GTGACCCAGT CCTCAGGCCA TGAGGACTCG CTGCAGTCAG ACTGTAATGG CCTTTCCCAG 
TTTGATCCCC AGGACCCAGA CAGGGCTCTT ACCCTGGTGC AGTCAGTGCT GACTCGGAGA 
CAGCAGGCTG TGCAGGACCT CAGGCAGCAG CTTTCAGGCT GCCAGGAGGC TATGAGCTTC 
TTGCAGCAGC AGCACGATCA GTGGGAGGAA GAGGGCAGGG CCCTGAGGGA AAAGCTACAG 
AAGCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGGCCTTCA GGGAGAGGTG 
GACTCTCTCA CTGGGGAGCG GGATGCTCTG GCAGGCCAGA CTGTGGACCT TCAGGGAGAG 
GTGGACTCTC TCAGCAGGGA GCGGGAGCTG CTTCAGAAGG CCAGAGGAGA GCTGCAGCAG 
CAACTGGAAG TGCTGGAGCA GGAAGCGTGG CGACTGCGGA GGATGAACAT GGAGCTGCAG 
CTGCAGGGGG ACTCAGCTCA AGGGGAGAAG CTGGAGCAGC AGGAGGAGTT GCACTTGGCT 
GTCCGGGAGA GGGAGCGCCT TCAGGAGACG CTGGTGGGCC TGGAAGCCAA GCAGTCAGAA 
TCACTAAGTG AGCTGCTCAC TCTTCGAGAA GCCCTGGAGT CAAGTCGCCT AGAAGGGGAG 
TTACTGAAGC AGGAGCGAGT GGAAGTGGCT GCAGCCTTGG CCAGGGCAGA ACAGTCCATT 
GTGGAGCTGT CAGGTTCTGA GAACAGCCTG AAGGCAGAGG TAGCTGATCT TCGAGCTGCA 
GCTGTCAAGC TTGGGGCCTT AAACGAGGCT TTGGCTTTAG ATAAAGTTGA ACTGAACCAG 
CAGCTTCTCC AGTTGGAGCA GGAGAACCAG TCTCTATGCA GCAGAGTGGA GGCCGCAGAG 
CAGCTGAGAA GCGCTTTGCA GGTGGACCTG GCGGAGGCAG AAAGGCGCAG GGAAGCCCTG 
TGGGAGAAGA AAACTCAGCT GGAGACTCAG CTGCAGAAGG CAGAGGAAGC GGGGGCTGAG 
CTACAGGCAG AGCTGAGGGG CACCCGAGAA GAAAAGGAAG AGCTTAAAGA TAAATTAAGT 
GAGGCACATC ACCAGCAAGA AACAGCTACA GCTCACCTAG AACAGCTGCA TCAGGACGCA 
GAAAGACAGG AAGAAACACT TGCTAGGGCA GTCCAGGAGA AGGAAGCCCT AGTACGGGAG 
AGGGCAGCTC TTGAGGTGCG CCTGCAGGCT GTGGAGCGGG ACCGACAGGA CCTCACTGAA 
CATGTCCTGG GGCTCAGGTC AGCCAAGGAG CAACTGGAGA GCAATCTATT TGAGGCCCAA 
CAGCAAAATT CTGTGATACA GGTCACCAAG GGGCAGCTGG AGGTCCAGAT TCAAACTATT 
ATCCAAGCCA AGGAAGTAAT TCAAGGGGAA GTGAAATGCC TGAAGCTGGA ACTGGATGCT 
GAGAGGACCC GGGCAGAGCA GGAGTGGGAT GCAGTAGCCA GGCAGCTAGC CCAAGCTGAG 
CAGGAGGGGC AAGCATCTCT GGAGCGACAG AAGGTGGCCC ACGAGGAGGA GGTGAACAGG 
CTGCAAGAGA AATGGGAGAA AGAGCGCTCC TGGCTGCAGC AGGAGCTGGA TAAGACCCTG 
GAAACCCTCG AGAGGGAGAG AGCAGAGCTG GAGACAAAGC TGAGAGAGCA GCAGACAGAA 
ATGGAAGCCA TCCGTGCACA GAGGGAAGAG GAACGAAGCC AGGCTGACAG CGCCCTGTAT 
CAAATGCAGC TAGAAACAGA GAAGGAGAGA GTATCCCTCC TGGAGACACT GCTGAGGACC 
CAGAAGGAGC TGGCAGATGC TAGTCAACAG CTGGAAAGGC TGAGACAGGA CATGAAGATT 
CAGAAGTTAA AGGAACAGGA GACCACTGGC ATGCTGCAGG CCCAGCTTCA GGAAACACAG 
CAGGAGCTGA AGGAGGCAGC CCAGCAGCAC AGGGACGACC TTGCTGCCTT CCAGAAGGAC 
AAGCTGGACC TGCAGAAGCA GGTAGAGGAC CTAATGTCTC AGCTGGTAGC CCACGATGAC 
TCCCAGAGGC TGGTGAAAGA AGAGATTGAA GAGAAGGTGA AAGTGGCTCA AGAATGCAGC 
CGGATTCAGA AGGAACTGGA GAAAGAGAAC GCCAGCCTGG CTCTGTCGCT GGTGGAAAAG 
GAAAAACGAC TTCTCATTTT ACAAGAAGCT GACTCTGTAA GACAGCAGGA GCTGAGCTCC 
TTGCGCCAGG ACATACAGGA GGCCCAGGAA GGGCAAAGAG AGCTGGGTGT ACAGGTGGAA 
CTATTGAGGC AGGAGGTGAA GGAAAAGGAG GCTGACTTTG TGGCCCGGGA AGCACAGCTG 
CTGGAGGAGT TGGAGGCCTC ACGGGTTGCA GAGCAGCAAC TTCGAGCGTC TTTGTGGGCC 
CAGGAAGCCA AGGCAACCCA GCTGCAGCTG CAGCTGCGCA GCACTGAGAG CCAGCTAGAG 
GCATTGGTTG CAGAACAACA GCCGGAGAAC CAGGCACAGG CACAGCTAGC TAGCCTCTGC 
TCTGTTCTGC AGCAGGCCCT GGGGTCTGCA TGTGAGAGTA GGCCTGAGCT GAGGGGTGGA 
GGCGACTCTG CTCCTACCTT ATGGGGCCCT GATCCAGATC AGAATGGAGC TTCCAGACTC 
TTTAAGAGAT GGTCGCTCCC AACGGCACTG TCACCGGAGG CTGTGGCACT AGCTCTCCAG 
AAGCTCCATC AGGACGTGTG GAAGGCTCGG CAGGCCCGGG ATGACCTAAG GGACCAGGTC 
CAGAAGCTGG TGCAGCGCCT GACTGACACG GAGGCCCAGA AGAGCCAGGT TCACTCGGAG 
CTGCAGGACC TGCAGAGACA GCTCTCTCAG AGCCAGGAAG AGAAATCCAA GTGGGAAGGG 
AGGCAGAACT CCCTGGAGTC TGAGCTGAGG GACTTGCACG AGACCGCGGC ATCCTTACAG 
AGTCGCCTCC GGCAGGCAGA ACTGCAAAAA ATGGAAGCTC AGAATGATCG AGAGTTGCTT 
CAGGCATCCA AGGAGAAGCT GTCAGCCCAG GTTGAACATC TGCAAGCATG TGTTGCAGAA 
GCCCAGGCTC AGGCAGATGC AGCTGCTGTC CTGGAAGAAG ATCTGCGAAC TGCTCGCTCT 
GCACTGAAAC TGAAAAACGA GGAGCTAGAG AGTGAACGGG AGAGAGCCCA GGCTTTACAA 
GAACAGGGTG AGCTGAAGGT GGCTCAGGGG AAAGCTTTGC AGGAGAACCT AGCCCTGCTG 
GCACAGACCC TGTCTAACAG AGAGCGGGAG GTCGAGACGT TGCAGGCTGA GGTTCAGGAA 
CTGGAGAAGC AGCGGGAGAT GCAGAAGGCT GCTCTGGAGC TGCTCTCCCT CGACCTGAAG 
AAGCGCAGTC GGGAGGTAGA CCTGCAACAA GAACAGATTC AGGAGCTGGA GCAGTGCAGG 
TCAGTCTTAG AGCACTTGCC CATGGCCGTC CAAGAGCGAG AGCAGAAACT GAGTGTGCAG 
CGGGACCAGA TCAGGGAACT GGAGAACGAT CGGGAGGCCC AGAGGAGTGT CCTGGAACAC 
CAGCTTCTGG ACCTGGAGCA GAAGGCTCAA GTGATTGAGT CCCAGAGGGG ACAGATTCAG 
GATCTGAAAA AGCAACTGGG GACACTGGAA TGCCTGGCCC TGGAACTGGA GGAAAGCCAT 
CACAAAGTGG AGAGCCAACA GAAGATGATC ACAGAACTAG AGGGCCAGAG GGAAATGCAG 
AGGGTGGCTC TAACCCACCT CACACTGGAC CTGGAAGAAA GGAGCCAGGA GCTGCAGGCA 
CAAAGCAGCC AGCTCCATGA GCTGGAGAAC CATAGCACCC ATCTGGCAAA AGAGCTGCAG 
GAGAGGGACC AGGAGGTGAC GTCCCAACGC CAGCAGATCG ATGAGCTGCA GAAGCAGCAG 
GAACAGCTGG CTCAGGCCCT GGAGAGGAAA GGCCAGGAGC TGGTGCTGCA GAAGGAAAGG 
ATTCAGGTTC TGGAAGATCA GAGGACGCTG CAGACCAAGA TCTTAGAGGA GGACCTAGAA 
CAGATCAAGC ATTCCTTGAG AGAACGCAGC CAGGAGCTGG CCTCTCAGTG GCAGCTGGTG 
CATGAGCGTG CCGACGATGG AAAGAGCCCG AGTAAAGGCC AGCGAGGGAG CCTGGAGCAC 
TTGAAGCTGA TCCTGCGTGA TAAGGAGAAG GAGGTAGAGT GCCAGCAGGA GCGCATCCAG 
GAACTTCAGG GCCACATGGG CCAGCTGGAA CAGCAGCTGC AGGGTCTGCA CAGGAAGGTG 
GGGGAGACTA GCCTGCTCCT GACCCATCGA GAGCAGGAGA CAGCAACCCT GCAGCAGCAT 
CTCCAGGAAG CCAAGGAACA AGGGGAACTG AGAGAGCAGG TACTTCAGGG TCAGCTGGAG 
GAGGCCCAGA GAGATCTGGC CCAGAGGGAC CATGAACTAG AGACCCTCCG GCAGGAAAAA 
CAGCAGACCC AGGACCAGGA GGAAAGCATG AAGCTAAAGA CAAGTGCTCT GCAGGCCGCT 
CTGGAGCAGG CCCATGCGAC ACTGAAGGAG CGTCAGGGGG AGCTGGAAGA ACACAGGGAA 
CAGGTGCGAA GGCTCCAGGA AGAACTAGAA GTGGAGGGAC GGCAGGTACG GGCCCTGGAG 
GAGGTGTTGG GGGACCTAAG GGCTGAGTCT CGGGAGCACG AGAAGGCTGT GCTGGCCCTT 
CAACAACGGT GTGCTGAGCA GGCCCAGGAA CATGAGGCAG AAGCCAGGAC CCTGCAGGAC 
AGCTGGCTGC AGGCCCAGGC CACTCTCACA GAACAAGAAC AAGAGCTAGC GGCACTGCGA 
GCGGAAAACC AGTATTCCCG CCGACAGGAA GAGGCTGCAG TTAGCCAGGC AGAGGCGCTG 
CAGGAGGCCC TCAGCAAAGC CCAGGCTGCC CTGCAGGAGA AAGAGCAGAG TCTGCTTGAA 
CAGGCTGAGT TGAGCCACAC CCTGGAGGCC AGCACTGCCG CCTTGCAAGC TACCCTGGAC 
ACCTGCCAGG CAAGTGCCCG GCAGCTGGAG GAGGCCCTGA GGATTCGGGA AGGTGAGATC 
CAGGCCCAGG CTCTCCAACA CCACGAGGTC ACGCAGCACC TCCAGCAGGA ACTTTGCCAG 
AAGAAAGAAG AGCTGAGGCA GCTACTAGAG AAGGCTGGGG CCCGGAGGAG TCAAGAAAAT 
GGAATCCAAG AGAAGCAAAG TCTGGAGCAA GAGAGACAAG AGGAAACAAG GAGGCTCCTT 
GAGAGCCTAA AGGAGCTACA GCTGACTGTA GCCCAAAGGG AAGAAGAGAT TCTGATGCTG 
AGGGAGGCCT CGTCCCCACG CCACAGAGCC CTCCCAGCCG AGAAGCCAGC TCTGCAACCG 
CTCCCAGCAC AACAGGAGTT AGAGCGGCTG CAGACTGCCC TAAGGCAAAC AGAGGCCAGG 
GAGATTGAAT GGAGGGAGAA GGCTCAGGAC CTGGCTCTGT CCCTGGCCCA AAGCAAGGCC 
AGCATCAGCA GTCTGCAGGA GATAACCATG TTCCTACAAG CCTCTGTTCT AGAGCGGGAA 
TCAGAACAAC AAAGACTACA GGAGGAGTTG GTACTCAGCA GACAAGCTCT GGAAGAGCAG 
CAGTCAGGTG GCCCACACTC AACCAGCAGA GCAGACCAGG GACCAAAAGT GGGACAGGGA 
AGCCAGTCTG GAGAGGTAGA GACTGAGCCT AGTCCTGGAG TGGAGGAGAA GGAACGGTTG 
ACACAGAGGC TTGAACGTCT GCAGCAAGCG GTGGCAGAGC TGGAAGTTGA CCGAAGCAAG 
CTGCAGTGCC ACAATGCGCA GCTGCGGACC GCCTTGGAGC AGGTGGAACG AGAACGGAGG 
AAGCTGAAGA GGGATTCCGT GCGTGCATCC CGGGCAGGAT CACTGGAGGC CAGAGAAACT 
ATGACTTCAT CCCCCACACA GCAGGATGGG AGAGGCAGTC AGAGGGGTTC CTCGGACAGC 
GTGCTTGTGG TTGAGCTTCA GAGAGAGGTA GCCCTGCTGC GAGCCCAGCT GGCCTTGGAG 
CGAAAGCAGA GGCAGGACTA CATTGCACGG TCCGTGCAGA CCAGCCGTGA GCTAGCAGGC 
CTGCATCACA GCCTGTCTCA CTCCCTCCTC ACCGTGGCCC AGGCCCCTGA GGCCACTGTT 
CTGGAGGCTG AGACCCGAAA GCTGGATGAG TCTCTGAATC AGAGTCTGAC ATCCCCAGGG 
CCATGTTTGC TACATCCCAG TCTGGACACT ACTCAAAATA CCCACAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( XM_030247663.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103523.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant X5, mRNA.

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CloneID	OMu87726
Clone ID Related Accession (Same CDS sequence)	XM_030247663.1 , XM_030247666.1 , XM_030247667.1
Accession Version	XM_030247663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAG CTACTGACAG AGACCTGACA GAGCTAAAGG CTGAACACGC AAGGCTTTCA 
GGGTCCCTGC TGACCTGCTG TCTGCGCCTG ACCCTGAGAG CACAGTCTCG GGAGTCCAGT 
GGATCTGGGA GAACAGAGGA GAGTGAGCCA GCCCGGCTGC TGCTGCTCGT CGCTAAGACC 
CAGGCGCTGG AGAAGGAAGC CCATGAGAAG AGCCAGGAGT TAATGCAGCT CAAGAGTCAC 
GGGGATCTAG AGAAGGCAGA GCTGCAGGAC CGAGTGACGG AACTCTCTGC TCTGCTAACC 
CAGTCTCAGA AGCAAAATGA AGATTATGAG AAGATGGTAA AGGCTCTAAG AGAAACAATG 
GAGATCCTGG AGACAAACCA TGCAGAGTTA ATGGAGCATG AGGCATCTCT CAGTAGGAAT 
GCTCAAGAGG AAAAGTTGTC TCTGCAGCAG GTGATCAAGG CTATAACCCA GGCACTGGCC 
TCAGTGGAAG AAGAAGACAC TGTGACCCAG TCCTCAGGCC ATGAGGACTC GCTGCAGTCA 
GACTGTAATG GCCTTTCCCA GTTTGATCCC CAGGACCCAG ACAGGGCTCT TACCCTGGTG 
CAGTCAGTGC TGACTCGGAG ACAGCAGGCT GTGCAGGACC TCAGGCAGCA GCTTTCAGGC 
TGCCAGGAGG CTATGAGCTT CTTGCAGCAG CAGCACGATC AGTGGGAGGA AGAGGGCAGG 
GCCCTGAGGG AAAAGCTACA GAAGCTCACT GGGGAGCGGG ATGCTCTGGC AGGCCAGACT 
GTGGGCCTTC AGGGAGAGGT GGACTCTCTC ACTGGGGAGC GGGATGCTCT GGCAGGCCAG 
ACTGTGGACC TTCAGGGAGA GGTGGACTCT CTCAGCAGGG AGCGGGAGCT GCTTCAGAAG 
GCCAGAGGAG AGCTGCAGCA GCAACTGGAA GTGCTGGAGC AGGAAGCGTG GCGACTGCGG 
AGGATGAACA TGGAGCTGCA GCTGCAGGGG GACTCAGCTC AAGGGGAGAA GCTGGAGCAG 
CAGGAGGAGT TGCACTTGGC TGTCCGGGAG AGGGAGCGCC TTCAGGAGAC GCTGGTGGGC 
CTGGAAGCCA AGCAGTCAGA ATCACTAAGT GAGCTGCTCA CTCTTCGAGA AGCCCTGGAG 
TCAAGTCGCC TAGAAGGGGA GTTACTGAAG CAGGAGCGAG TGGAAGTGGC TGCAGCCTTG 
GCCAGGGCAG AACAGTCCAT TGTGGAGCTG TCAGGTTCTG AGAACAGCCT GAAGGCAGAG 
GTAGCTGATC TTCGAGCTGC AGCTGTCAAG CTTGGGGCCT TAAACGAGGC TTTGGCTTTA 
GATAAAGTTG AACTGAACCA GCAGCTTCTC CAGTTGGAGC AGGAGAACCA GTCTCTATGC 
AGCAGAGTGG AGGCCGCAGA GCAGCTGAGA AGCGCTTTGC AGGTGGACCT GGCGGAGGCA 
GAAAGGCGCA GGGAAGCCCT GTGGGAGAAG AAAACTCAGC TGGAGACTCA GCTGCAGAAG 
GCAGAGGAAG CGGGGGCTGA GCTACAGGCA GAGCTGAGGG GCACCCGAGA AGAAAAGGAA 
GAGCTTAAAG ATAAATTAAG TGAGGCACAT CACCAGCAAG AAACAGCTAC AGCTCACCTA 
GAACAGCTGC ATCAGGACGC AGAAAGACAG GAAGAAACAC TTGCTAGGGC AGTCCAGGAG 
AAGGAAGCCC TAGTACGGGA GAGGGCAGCT CTTGAGGTGC GCCTGCAGGC TGTGGAGCGG 
GACCGACAGG ACCTCACTGA ACATGTCCTG GGGCTCAGGT CAGCCAAGGA GCAACTGGAG 
AGCAATCTAT TTGAGGCCCA ACAGCAAAAT TCTGTGATAC AGGTCACCAA GGGGCAGCTG 
GAGGTCCAGA TTCAAACTAT TATCCAAGCC AAGGAAGTAA TTCAAGGGGA AGTGAAATGC 
CTGAAGCTGG AACTGGATGC TGAGAGGACC CGGGCAGAGC AGGAGTGGGA TGCAGTAGCC 
AGGCAGCTAG CCCAAGCTGA GCAGGAGGGG CAAGCATCTC TGGAGCGACA GAAGGTGGCC 
CACGAGGAGG AGGTGAACAG GCTGCAAGAG AAATGGGAGA AAGAGCGCTC CTGGCTGCAG 
CAGGAGCTGG ATAAGACCCT GGAAACCCTC GAGAGGGAGA GAGCAGAGCT GGAGACAAAG 
CTGAGAGAGC AGCAGACAGA AATGGAAGCC ATCCGTGCAC AGAGGGAAGA GGAACGAAGC 
CAGGCTGACA GCGCCCTGTA TCAAATGCAG CTAGAAACAG AGAAGGAGAG AGTATCCCTC 
CTGGAGACAC TGCTGAGGAC CCAGAAGGAG CTGGCAGATG CTAGTCAACA GCTGGAAAGG 
CTGAGACAGG ACATGAAGAT TCAGAAGTTA AAGGAACAGG AGACCACTGG CATGCTGCAG 
GCCCAGCTTC AGGAAACACA GCAGGAGCTG AAGGAGGCAG CCCAGCAGCA CAGGGACGAC 
CTTGCTGCCT TCCAGAAGGA CAAGCTGGAC CTGCAGAAGC AGGTAGAGGA CCTAATGTCT 
CAGCTGGTAG CCCACGATGA CTCCCAGAGG CTGGTGAAAG AAGAGATTGA AGAGAAGGTG 
AAAGTGGCTC AAGAATGCAG CCGGATTCAG AAGGAACTGG AGAAAGAGAA CGCCAGCCTG 
GCTCTGTCGC TGGTGGAAAA GGAAAAACGA CTTCTCATTT TACAAGAAGC TGACTCTGTA 
AGACAGCAGG AGCTGAGCTC CTTGCGCCAG GACATACAGG AGGCCCAGGA AGGGCAAAGA 
GAGCTGGGTG TACAGGTGGA ACTATTGAGG CAGGAGGTGA AGGAAAAGGA GGCTGACTTT 
GTGGCCCGGG AAGCACAGCT GCTGGAGGAG TTGGAGGCCT CACGGGTTGC AGAGCAGCAA 
CTTCGAGCGT CTTTGTGGGC CCAGGAAGCC AAGGCAACCC AGCTGCAGCT GCAGCTGCGC 
AGCACTGAGA GCCAGCTAGA GGCATTGGTT GCAGAACAAC AGCCGGAGAA CCAGGCACAG 
GCACAGCTAG CTAGCCTCTG CTCTGTTCTG CAGCAGGCCC TGGGGTCTGC ATGTGAGAGT 
AGGCCTGAGC TGAGGGGTGG AGGCGACTCT GCTCCTACCT TATGGGGCCC TGATCCAGAT 
CAGAATGGAG CTTCCAGACT CTTTAAGAGA TGGTCGCTCC CAACGGCACT GTCACCGGAG 
GCTGTGGCAC TAGCTCTCCA GAAGCTCCAT CAGGACGTGT GGAAGGCTCG GCAGGCCCGG 
GATGACCTAA GGGACCAGGT CCAGAAGCTG GTGCAGCGCC TGACTGACAC GGAGGCCCAG 
AAGAGCCAGG TTCACTCGGA GCTGCAGGAC CTGCAGAGAC AGCTCTCTCA GAGCCAGGAA 
GAGAAATCCA AGTGGGAAGG GAGGCAGAAC TCCCTGGAGT CTGAGCTGAG GGACTTGCAC 
GAGACCGCGG CATCCTTACA GAGTCGCCTC CGGCAGGCAG AACTGCAAAA AATGGAAGCT 
CAGAATGATC GAGAGTTGCT TCAGGCATCC AAGGAGAAGC TGTCAGCCCA GGTTGAACAT 
CTGCAAGCAT GTGTTGCAGA AGCCCAGGCT CAGGCAGATG CAGCTGCTGT CCTGGAAGAA 
GATCTGCGAA CTGCTCGCTC TGCACTGAAA CTGAAAAACG AGGAGCTAGA GAGTGAACGG 
GAGAGAGCCC AGGCTTTACA AGAACAGGGT GAGCTGAAGG TGGCTCAGGG GAAAGCTTTG 
CAGGAGAACC TAGCCCTGCT GGCACAGACC CTGTCTAACA GAGAGCGGGA GGTCGAGACG 
TTGCAGGCTG AGGTTCAGGA ACTGGAGAAG CAGCGGGAGA TGCAGAAGGC TGCTCTGGAG 
CTGCTCTCCC TCGACCTGAA GAAGCGCAGT CGGGAGGTAG ACCTGCAACA AGAACAGATT 
CAGGAGCTGG AGCAGTGCAG GTCAGTCTTA GAGCACTTGC CCATGGCCGT CCAAGAGCGA 
GAGCAGAAAC TGAGTGTGCA GCGGGACCAG ATCAGGGAAC TGGAGAACGA TCGGGAGGCC 
CAGAGGAGTG TCCTGGAACA CCAGCTTCTG GACCTGGAGC AGAAGGCTCA AGTGATTGAG 
TCCCAGAGGG GACAGATTCA GGATCTGAAA AAGCAACTGG GGACACTGGA ATGCCTGGCC 
CTGGAACTGG AGGAAAGCCA TCACAAAGTG GAGAGCCAAC AGAAGATGAT CACAGAACTA 
GAGGGCCAGA GGGAAATGCA GAGGGTGGCT CTAACCCACC TCACACTGGA CCTGGAAGAA 
AGGAGCCAGG AGCTGCAGGC ACAAAGCAGC CAGCTCCATG AGCTGGAGAA CCATAGCACC 
CATCTGGCAA AAGAGCTGCA GGAGAGGGAC CAGGAGGTGA CGTCCCAACG CCAGCAGATC 
GATGAGCTGC AGAAGCAGCA GGAACAGCTG GCTCAGGCCC TGGAGAGGAA AGGCCAGGAG 
CTGGTGCTGC AGAAGGAAAG GATTCAGGTT CTGGAAGATC AGAGGACGCT GCAGACCAAG 
ATCTTAGAGG AGGACCTAGA ACAGATCAAG CATTCCTTGA GAGAACGCAG CCAGGAGCTG 
GCCTCTCAGT GGCAGCTGGT GCATGAGCGT GCCGACGATG GAAAGAGCCC GAGTAAAGGC 
CAGCGAGGGA GCCTGGAGCA CTTGAAGCTG ATCCTGCGTG ATAAGGAGAA GGAGGTAGAG 
TGCCAGCAGG AGCGCATCCA GGAACTTCAG GGCCACATGG GCCAGCTGGA ACAGCAGCTG 
CAGGGTCTGC ACAGGAAGGT GGGGGAGACT AGCCTGCTCC TGACCCATCG AGAGCAGGAG 
ACAGCAACCC TGCAGCAGCA TCTCCAGGAA GCCAAGGAAC AAGGGGAACT GAGAGAGCAG 
GTACTTCAGG GTCAGCTGGA GGAGGCCCAG AGAGATCTGG CCCAGAGGGA CCATGAACTA 
GAGACCCTCC GGCAGGAAAA ACAGCAGACC CAGGACCAGG AGGAAAGCAT GAAGCTAAAG 
ACAAGTGCTC TGCAGGCCGC TCTGGAGCAG GCCCATGCGA CACTGAAGGA GCGTCAGGGG 
GAGCTGGAAG AACACAGGGA ACAGGTGCGA AGGCTCCAGG AAGAACTAGA AGTGGAGGGA 
CGGCAGGTAC GGGCCCTGGA GGAGGTGTTG GGGGACCTAA GGGCTGAGTC TCGGGAGCAC 
GAGAAGGCTG TGCTGGCCCT TCAACAACGG TGTGCTGAGC AGGCCCAGGA ACATGAGGCA 
GAAGCCAGGA CCCTGCAGGA CAGCTGGCTG CAGGCCCAGG CCACTCTCAC AGAACAAGAA 
CAAGAGCTAG CGGCACTGCG AGCGGAAAAC CAGTATTCCC GCCGACAGGA AGAGGCTGCA 
GTTAGCCAGG CAGAGGCGCT GCAGGAGGCC CTCAGCAAAG CCCAGGCTGC CCTGCAGGAG 
AAAGAGCAGA GTCTGCTTGA ACAGGCTGAG TTGAGCCACA CCCTGGAGGC CAGCACTGCC 
GCCTTGCAAG CTACCCTGGA CACCTGCCAG GCAAGTGCCC GGCAGCTGGA GGAGGCCCTG 
AGGATTCGGG AAGGTGAGAT CCAGGCCCAG GCTCTCCAAC ACCACGAGGT CACGCAGCAC 
CTCCAGCAGG AACTTTGCCA GAAGAAAGAA GAGCTGAGGC AGCTACTAGA GAAGGCTGGG 
GCCCGGAGGA GTCAAGAAAA TGGAATCCAA GAGAAGCAAA GTCTGGAGCA AGAGAGACAA 
GAGGAAACAA GGAGGCTCCT TGAGAGCCTA AAGGAGCTAC AGCTGACTGT AGCCCAAAGG 
GAAGAAGAGA TTCTGATGCT GAGGGAGGCC TCGTCCCCAC GCCACAGAGC CCTCCCAGCC 
GAGAAGCCAG CTCTGCAACC GCTCCCAGCA CAACAGGAGT TAGAGCGGCT GCAGACTGCC 
CTAAGGCAAA CAGAGGCCAG GGAGATTGAA TGGAGGGAGA AGGCTCAGGA CCTGGCTCTG 
TCCCTGGCCC AAAGCAAGGC CAGCATCAGC AGTCTGCAGG AGATAACCAT GTTCCTACAA 
GCCTCTGTTC TAGAGCGGGA ATCAGAACAA CAAAGACTAC AGGAGGAGTT GGTACTCAGC 
AGACAAGCTC TGGAAGAGCA GCAGTCAGGT GGCCCACACT CAACCAGCAG AGCAGACCAG 
GGACCAAAAG TGGGACAGGG AAGCCAGTCT GGAGAGGTAG AGACTGAGCC TAGTCCTGGA 
GTGGAGGAGA AGGAACGGTT GACACAGAGG CTTGAACGTC TGCAGCAAGC GGTGGCAGAG 
CTGGAAGTTG ACCGAAGCAA GCTGCAGTGC CACAATGCGC AGCTGCGGAC CGCCTTGGAG 
CAGGTGGAAC GAGAACGGAG GAAGCTGAAG AGGGATTCCG TGCGTGCATC CCGGGCAGGA 
TCACTGGAGG CCAGAGAAAC TATGACTTCA TCCCCCACAC AGCAGGATGG GAGAGGCAGT 
CAGAGGGGTT CCTCGGACAG CGTGCTTGTG GTTGAGCTTC AGAGAGAGGT AGCCCTGCTG 
CGAGCCCAGC TGGCCTTGGA GCGAAAGCAG AGGCAGGACT ACATTGCACG GTCCGTGCAG 
ACCAGCCGTG AGCTAGCAGG CCTGCATCAC AGCCTGTCTC ACTCCCTCCT CACCGTGGCC 
CAGGCCCCTG AGGCCACTGT TCTGGAGGCT GAGACCCGAA AGCTGGATGA GTCTCTGAAT 
CAGAGTCTGA CATCCCCAGG GCCATGTTTG CTACATCCCA GTCTGGACAC TACTCAAAAT 
ACCCACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103523.1
CDS	935..7666
Translation	MKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	XM_030247663.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247663.1

ORF Insert Sequence:

ATGAAGTCAG CTACTGACAG AGACCTGACA GAGCTAAAGG CTGAACACGC AAGGCTTTCA 
GGGTCCCTGC TGACCTGCTG TCTGCGCCTG ACCCTGAGAG CACAGTCTCG GGAGTCCAGT 
GGATCTGGGA GAACAGAGGA GAGTGAGCCA GCCCGGCTGC TGCTGCTCGT CGCTAAGACC 
CAGGCGCTGG AGAAGGAAGC CCATGAGAAG AGCCAGGAGT TAATGCAGCT CAAGAGTCAC 
GGGGATCTAG AGAAGGCAGA GCTGCAGGAC CGAGTGACGG AACTCTCTGC TCTGCTAACC 
CAGTCTCAGA AGCAAAATGA AGATTATGAG AAGATGGTAA AGGCTCTAAG AGAAACAATG 
GAGATCCTGG AGACAAACCA TGCAGAGTTA ATGGAGCATG AGGCATCTCT CAGTAGGAAT 
GCTCAAGAGG AAAAGTTGTC TCTGCAGCAG GTGATCAAGG CTATAACCCA GGCACTGGCC 
TCAGTGGAAG AAGAAGACAC TGTGACCCAG TCCTCAGGCC ATGAGGACTC GCTGCAGTCA 
GACTGTAATG GCCTTTCCCA GTTTGATCCC CAGGACCCAG ACAGGGCTCT TACCCTGGTG 
CAGTCAGTGC TGACTCGGAG ACAGCAGGCT GTGCAGGACC TCAGGCAGCA GCTTTCAGGC 
TGCCAGGAGG CTATGAGCTT CTTGCAGCAG CAGCACGATC AGTGGGAGGA AGAGGGCAGG 
GCCCTGAGGG AAAAGCTACA GAAGCTCACT GGGGAGCGGG ATGCTCTGGC AGGCCAGACT 
GTGGGCCTTC AGGGAGAGGT GGACTCTCTC ACTGGGGAGC GGGATGCTCT GGCAGGCCAG 
ACTGTGGACC TTCAGGGAGA GGTGGACTCT CTCAGCAGGG AGCGGGAGCT GCTTCAGAAG 
GCCAGAGGAG AGCTGCAGCA GCAACTGGAA GTGCTGGAGC AGGAAGCGTG GCGACTGCGG 
AGGATGAACA TGGAGCTGCA GCTGCAGGGG GACTCAGCTC AAGGGGAGAA GCTGGAGCAG 
CAGGAGGAGT TGCACTTGGC TGTCCGGGAG AGGGAGCGCC TTCAGGAGAC GCTGGTGGGC 
CTGGAAGCCA AGCAGTCAGA ATCACTAAGT GAGCTGCTCA CTCTTCGAGA AGCCCTGGAG 
TCAAGTCGCC TAGAAGGGGA GTTACTGAAG CAGGAGCGAG TGGAAGTGGC TGCAGCCTTG 
GCCAGGGCAG AACAGTCCAT TGTGGAGCTG TCAGGTTCTG AGAACAGCCT GAAGGCAGAG 
GTAGCTGATC TTCGAGCTGC AGCTGTCAAG CTTGGGGCCT TAAACGAGGC TTTGGCTTTA 
GATAAAGTTG AACTGAACCA GCAGCTTCTC CAGTTGGAGC AGGAGAACCA GTCTCTATGC 
AGCAGAGTGG AGGCCGCAGA GCAGCTGAGA AGCGCTTTGC AGGTGGACCT GGCGGAGGCA 
GAAAGGCGCA GGGAAGCCCT GTGGGAGAAG AAAACTCAGC TGGAGACTCA GCTGCAGAAG 
GCAGAGGAAG CGGGGGCTGA GCTACAGGCA GAGCTGAGGG GCACCCGAGA AGAAAAGGAA 
GAGCTTAAAG ATAAATTAAG TGAGGCACAT CACCAGCAAG AAACAGCTAC AGCTCACCTA 
GAACAGCTGC ATCAGGACGC AGAAAGACAG GAAGAAACAC TTGCTAGGGC AGTCCAGGAG 
AAGGAAGCCC TAGTACGGGA GAGGGCAGCT CTTGAGGTGC GCCTGCAGGC TGTGGAGCGG 
GACCGACAGG ACCTCACTGA ACATGTCCTG GGGCTCAGGT CAGCCAAGGA GCAACTGGAG 
AGCAATCTAT TTGAGGCCCA ACAGCAAAAT TCTGTGATAC AGGTCACCAA GGGGCAGCTG 
GAGGTCCAGA TTCAAACTAT TATCCAAGCC AAGGAAGTAA TTCAAGGGGA AGTGAAATGC 
CTGAAGCTGG AACTGGATGC TGAGAGGACC CGGGCAGAGC AGGAGTGGGA TGCAGTAGCC 
AGGCAGCTAG CCCAAGCTGA GCAGGAGGGG CAAGCATCTC TGGAGCGACA GAAGGTGGCC 
CACGAGGAGG AGGTGAACAG GCTGCAAGAG AAATGGGAGA AAGAGCGCTC CTGGCTGCAG 
CAGGAGCTGG ATAAGACCCT GGAAACCCTC GAGAGGGAGA GAGCAGAGCT GGAGACAAAG 
CTGAGAGAGC AGCAGACAGA AATGGAAGCC ATCCGTGCAC AGAGGGAAGA GGAACGAAGC 
CAGGCTGACA GCGCCCTGTA TCAAATGCAG CTAGAAACAG AGAAGGAGAG AGTATCCCTC 
CTGGAGACAC TGCTGAGGAC CCAGAAGGAG CTGGCAGATG CTAGTCAACA GCTGGAAAGG 
CTGAGACAGG ACATGAAGAT TCAGAAGTTA AAGGAACAGG AGACCACTGG CATGCTGCAG 
GCCCAGCTTC AGGAAACACA GCAGGAGCTG AAGGAGGCAG CCCAGCAGCA CAGGGACGAC 
CTTGCTGCCT TCCAGAAGGA CAAGCTGGAC CTGCAGAAGC AGGTAGAGGA CCTAATGTCT 
CAGCTGGTAG CCCACGATGA CTCCCAGAGG CTGGTGAAAG AAGAGATTGA AGAGAAGGTG 
AAAGTGGCTC AAGAATGCAG CCGGATTCAG AAGGAACTGG AGAAAGAGAA CGCCAGCCTG 
GCTCTGTCGC TGGTGGAAAA GGAAAAACGA CTTCTCATTT TACAAGAAGC TGACTCTGTA 
AGACAGCAGG AGCTGAGCTC CTTGCGCCAG GACATACAGG AGGCCCAGGA AGGGCAAAGA 
GAGCTGGGTG TACAGGTGGA ACTATTGAGG CAGGAGGTGA AGGAAAAGGA GGCTGACTTT 
GTGGCCCGGG AAGCACAGCT GCTGGAGGAG TTGGAGGCCT CACGGGTTGC AGAGCAGCAA 
CTTCGAGCGT CTTTGTGGGC CCAGGAAGCC AAGGCAACCC AGCTGCAGCT GCAGCTGCGC 
AGCACTGAGA GCCAGCTAGA GGCATTGGTT GCAGAACAAC AGCCGGAGAA CCAGGCACAG 
GCACAGCTAG CTAGCCTCTG CTCTGTTCTG CAGCAGGCCC TGGGGTCTGC ATGTGAGAGT 
AGGCCTGAGC TGAGGGGTGG AGGCGACTCT GCTCCTACCT TATGGGGCCC TGATCCAGAT 
CAGAATGGAG CTTCCAGACT CTTTAAGAGA TGGTCGCTCC CAACGGCACT GTCACCGGAG 
GCTGTGGCAC TAGCTCTCCA GAAGCTCCAT CAGGACGTGT GGAAGGCTCG GCAGGCCCGG 
GATGACCTAA GGGACCAGGT CCAGAAGCTG GTGCAGCGCC TGACTGACAC GGAGGCCCAG 
AAGAGCCAGG TTCACTCGGA GCTGCAGGAC CTGCAGAGAC AGCTCTCTCA GAGCCAGGAA 
GAGAAATCCA AGTGGGAAGG GAGGCAGAAC TCCCTGGAGT CTGAGCTGAG GGACTTGCAC 
GAGACCGCGG CATCCTTACA GAGTCGCCTC CGGCAGGCAG AACTGCAAAA AATGGAAGCT 
CAGAATGATC GAGAGTTGCT TCAGGCATCC AAGGAGAAGC TGTCAGCCCA GGTTGAACAT 
CTGCAAGCAT GTGTTGCAGA AGCCCAGGCT CAGGCAGATG CAGCTGCTGT CCTGGAAGAA 
GATCTGCGAA CTGCTCGCTC TGCACTGAAA CTGAAAAACG AGGAGCTAGA GAGTGAACGG 
GAGAGAGCCC AGGCTTTACA AGAACAGGGT GAGCTGAAGG TGGCTCAGGG GAAAGCTTTG 
CAGGAGAACC TAGCCCTGCT GGCACAGACC CTGTCTAACA GAGAGCGGGA GGTCGAGACG 
TTGCAGGCTG AGGTTCAGGA ACTGGAGAAG CAGCGGGAGA TGCAGAAGGC TGCTCTGGAG 
CTGCTCTCCC TCGACCTGAA GAAGCGCAGT CGGGAGGTAG ACCTGCAACA AGAACAGATT 
CAGGAGCTGG AGCAGTGCAG GTCAGTCTTA GAGCACTTGC CCATGGCCGT CCAAGAGCGA 
GAGCAGAAAC TGAGTGTGCA GCGGGACCAG ATCAGGGAAC TGGAGAACGA TCGGGAGGCC 
CAGAGGAGTG TCCTGGAACA CCAGCTTCTG GACCTGGAGC AGAAGGCTCA AGTGATTGAG 
TCCCAGAGGG GACAGATTCA GGATCTGAAA AAGCAACTGG GGACACTGGA ATGCCTGGCC 
CTGGAACTGG AGGAAAGCCA TCACAAAGTG GAGAGCCAAC AGAAGATGAT CACAGAACTA 
GAGGGCCAGA GGGAAATGCA GAGGGTGGCT CTAACCCACC TCACACTGGA CCTGGAAGAA 
AGGAGCCAGG AGCTGCAGGC ACAAAGCAGC CAGCTCCATG AGCTGGAGAA CCATAGCACC 
CATCTGGCAA AAGAGCTGCA GGAGAGGGAC CAGGAGGTGA CGTCCCAACG CCAGCAGATC 
GATGAGCTGC AGAAGCAGCA GGAACAGCTG GCTCAGGCCC TGGAGAGGAA AGGCCAGGAG 
CTGGTGCTGC AGAAGGAAAG GATTCAGGTT CTGGAAGATC AGAGGACGCT GCAGACCAAG 
ATCTTAGAGG AGGACCTAGA ACAGATCAAG CATTCCTTGA GAGAACGCAG CCAGGAGCTG 
GCCTCTCAGT GGCAGCTGGT GCATGAGCGT GCCGACGATG GAAAGAGCCC GAGTAAAGGC 
CAGCGAGGGA GCCTGGAGCA CTTGAAGCTG ATCCTGCGTG ATAAGGAGAA GGAGGTAGAG 
TGCCAGCAGG AGCGCATCCA GGAACTTCAG GGCCACATGG GCCAGCTGGA ACAGCAGCTG 
CAGGGTCTGC ACAGGAAGGT GGGGGAGACT AGCCTGCTCC TGACCCATCG AGAGCAGGAG 
ACAGCAACCC TGCAGCAGCA TCTCCAGGAA GCCAAGGAAC AAGGGGAACT GAGAGAGCAG 
GTACTTCAGG GTCAGCTGGA GGAGGCCCAG AGAGATCTGG CCCAGAGGGA CCATGAACTA 
GAGACCCTCC GGCAGGAAAA ACAGCAGACC CAGGACCAGG AGGAAAGCAT GAAGCTAAAG 
ACAAGTGCTC TGCAGGCCGC TCTGGAGCAG GCCCATGCGA CACTGAAGGA GCGTCAGGGG 
GAGCTGGAAG AACACAGGGA ACAGGTGCGA AGGCTCCAGG AAGAACTAGA AGTGGAGGGA 
CGGCAGGTAC GGGCCCTGGA GGAGGTGTTG GGGGACCTAA GGGCTGAGTC TCGGGAGCAC 
GAGAAGGCTG TGCTGGCCCT TCAACAACGG TGTGCTGAGC AGGCCCAGGA ACATGAGGCA 
GAAGCCAGGA CCCTGCAGGA CAGCTGGCTG CAGGCCCAGG CCACTCTCAC AGAACAAGAA 
CAAGAGCTAG CGGCACTGCG AGCGGAAAAC CAGTATTCCC GCCGACAGGA AGAGGCTGCA 
GTTAGCCAGG CAGAGGCGCT GCAGGAGGCC CTCAGCAAAG CCCAGGCTGC CCTGCAGGAG 
AAAGAGCAGA GTCTGCTTGA ACAGGCTGAG TTGAGCCACA CCCTGGAGGC CAGCACTGCC 
GCCTTGCAAG CTACCCTGGA CACCTGCCAG GCAAGTGCCC GGCAGCTGGA GGAGGCCCTG 
AGGATTCGGG AAGGTGAGAT CCAGGCCCAG GCTCTCCAAC ACCACGAGGT CACGCAGCAC 
CTCCAGCAGG AACTTTGCCA GAAGAAAGAA GAGCTGAGGC AGCTACTAGA GAAGGCTGGG 
GCCCGGAGGA GTCAAGAAAA TGGAATCCAA GAGAAGCAAA GTCTGGAGCA AGAGAGACAA 
GAGGAAACAA GGAGGCTCCT TGAGAGCCTA AAGGAGCTAC AGCTGACTGT AGCCCAAAGG 
GAAGAAGAGA TTCTGATGCT GAGGGAGGCC TCGTCCCCAC GCCACAGAGC CCTCCCAGCC 
GAGAAGCCAG CTCTGCAACC GCTCCCAGCA CAACAGGAGT TAGAGCGGCT GCAGACTGCC 
CTAAGGCAAA CAGAGGCCAG GGAGATTGAA TGGAGGGAGA AGGCTCAGGA CCTGGCTCTG 
TCCCTGGCCC AAAGCAAGGC CAGCATCAGC AGTCTGCAGG AGATAACCAT GTTCCTACAA 
GCCTCTGTTC TAGAGCGGGA ATCAGAACAA CAAAGACTAC AGGAGGAGTT GGTACTCAGC 
AGACAAGCTC TGGAAGAGCA GCAGTCAGGT GGCCCACACT CAACCAGCAG AGCAGACCAG 
GGACCAAAAG TGGGACAGGG AAGCCAGTCT GGAGAGGTAG AGACTGAGCC TAGTCCTGGA 
GTGGAGGAGA AGGAACGGTT GACACAGAGG CTTGAACGTC TGCAGCAAGC GGTGGCAGAG 
CTGGAAGTTG ACCGAAGCAA GCTGCAGTGC CACAATGCGC AGCTGCGGAC CGCCTTGGAG 
CAGGTGGAAC GAGAACGGAG GAAGCTGAAG AGGGATTCCG TGCGTGCATC CCGGGCAGGA 
TCACTGGAGG CCAGAGAAAC TATGACTTCA TCCCCCACAC AGCAGGATGG GAGAGGCAGT 
CAGAGGGGTT CCTCGGACAG CGTGCTTGTG GTTGAGCTTC AGAGAGAGGT AGCCCTGCTG 
CGAGCCCAGC TGGCCTTGGA GCGAAAGCAG AGGCAGGACT ACATTGCACG GTCCGTGCAG 
ACCAGCCGTG AGCTAGCAGG CCTGCATCAC AGCCTGTCTC ACTCCCTCCT CACCGTGGCC 
CAGGCCCCTG AGGCCACTGT TCTGGAGGCT GAGACCCGAA AGCTGGATGA GTCTCTGAAT 
CAGAGTCTGA CATCCCCAGG GCCATGTTTG CTACATCCCA GTCTGGACAC TACTCAAAAT 
ACCCACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( XM_030247666.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103526.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant X6, mRNA.

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CloneID	OMu87726
Clone ID Related Accession (Same CDS sequence)	XM_030247663.1 , XM_030247666.1 , XM_030247667.1
Accession Version	XM_030247666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAG CTACTGACAG AGACCTGACA GAGCTAAAGG CTGAACACGC AAGGCTTTCA 
GGGTCCCTGC TGACCTGCTG TCTGCGCCTG ACCCTGAGAG CACAGTCTCG GGAGTCCAGT 
GGATCTGGGA GAACAGAGGA GAGTGAGCCA GCCCGGCTGC TGCTGCTCGT CGCTAAGACC 
CAGGCGCTGG AGAAGGAAGC CCATGAGAAG AGCCAGGAGT TAATGCAGCT CAAGAGTCAC 
GGGGATCTAG AGAAGGCAGA GCTGCAGGAC CGAGTGACGG AACTCTCTGC TCTGCTAACC 
CAGTCTCAGA AGCAAAATGA AGATTATGAG AAGATGGTAA AGGCTCTAAG AGAAACAATG 
GAGATCCTGG AGACAAACCA TGCAGAGTTA ATGGAGCATG AGGCATCTCT CAGTAGGAAT 
GCTCAAGAGG AAAAGTTGTC TCTGCAGCAG GTGATCAAGG CTATAACCCA GGCACTGGCC 
TCAGTGGAAG AAGAAGACAC TGTGACCCAG TCCTCAGGCC ATGAGGACTC GCTGCAGTCA 
GACTGTAATG GCCTTTCCCA GTTTGATCCC CAGGACCCAG ACAGGGCTCT TACCCTGGTG 
CAGTCAGTGC TGACTCGGAG ACAGCAGGCT GTGCAGGACC TCAGGCAGCA GCTTTCAGGC 
TGCCAGGAGG CTATGAGCTT CTTGCAGCAG CAGCACGATC AGTGGGAGGA AGAGGGCAGG 
GCCCTGAGGG AAAAGCTACA GAAGCTCACT GGGGAGCGGG ATGCTCTGGC AGGCCAGACT 
GTGGGCCTTC AGGGAGAGGT GGACTCTCTC ACTGGGGAGC GGGATGCTCT GGCAGGCCAG 
ACTGTGGACC TTCAGGGAGA GGTGGACTCT CTCAGCAGGG AGCGGGAGCT GCTTCAGAAG 
GCCAGAGGAG AGCTGCAGCA GCAACTGGAA GTGCTGGAGC AGGAAGCGTG GCGACTGCGG 
AGGATGAACA TGGAGCTGCA GCTGCAGGGG GACTCAGCTC AAGGGGAGAA GCTGGAGCAG 
CAGGAGGAGT TGCACTTGGC TGTCCGGGAG AGGGAGCGCC TTCAGGAGAC GCTGGTGGGC 
CTGGAAGCCA AGCAGTCAGA ATCACTAAGT GAGCTGCTCA CTCTTCGAGA AGCCCTGGAG 
TCAAGTCGCC TAGAAGGGGA GTTACTGAAG CAGGAGCGAG TGGAAGTGGC TGCAGCCTTG 
GCCAGGGCAG AACAGTCCAT TGTGGAGCTG TCAGGTTCTG AGAACAGCCT GAAGGCAGAG 
GTAGCTGATC TTCGAGCTGC AGCTGTCAAG CTTGGGGCCT TAAACGAGGC TTTGGCTTTA 
GATAAAGTTG AACTGAACCA GCAGCTTCTC CAGTTGGAGC AGGAGAACCA GTCTCTATGC 
AGCAGAGTGG AGGCCGCAGA GCAGCTGAGA AGCGCTTTGC AGGTGGACCT GGCGGAGGCA 
GAAAGGCGCA GGGAAGCCCT GTGGGAGAAG AAAACTCAGC TGGAGACTCA GCTGCAGAAG 
GCAGAGGAAG CGGGGGCTGA GCTACAGGCA GAGCTGAGGG GCACCCGAGA AGAAAAGGAA 
GAGCTTAAAG ATAAATTAAG TGAGGCACAT CACCAGCAAG AAACAGCTAC AGCTCACCTA 
GAACAGCTGC ATCAGGACGC AGAAAGACAG GAAGAAACAC TTGCTAGGGC AGTCCAGGAG 
AAGGAAGCCC TAGTACGGGA GAGGGCAGCT CTTGAGGTGC GCCTGCAGGC TGTGGAGCGG 
GACCGACAGG ACCTCACTGA ACATGTCCTG GGGCTCAGGT CAGCCAAGGA GCAACTGGAG 
AGCAATCTAT TTGAGGCCCA ACAGCAAAAT TCTGTGATAC AGGTCACCAA GGGGCAGCTG 
GAGGTCCAGA TTCAAACTAT TATCCAAGCC AAGGAAGTAA TTCAAGGGGA AGTGAAATGC 
CTGAAGCTGG AACTGGATGC TGAGAGGACC CGGGCAGAGC AGGAGTGGGA TGCAGTAGCC 
AGGCAGCTAG CCCAAGCTGA GCAGGAGGGG CAAGCATCTC TGGAGCGACA GAAGGTGGCC 
CACGAGGAGG AGGTGAACAG GCTGCAAGAG AAATGGGAGA AAGAGCGCTC CTGGCTGCAG 
CAGGAGCTGG ATAAGACCCT GGAAACCCTC GAGAGGGAGA GAGCAGAGCT GGAGACAAAG 
CTGAGAGAGC AGCAGACAGA AATGGAAGCC ATCCGTGCAC AGAGGGAAGA GGAACGAAGC 
CAGGCTGACA GCGCCCTGTA TCAAATGCAG CTAGAAACAG AGAAGGAGAG AGTATCCCTC 
CTGGAGACAC TGCTGAGGAC CCAGAAGGAG CTGGCAGATG CTAGTCAACA GCTGGAAAGG 
CTGAGACAGG ACATGAAGAT TCAGAAGTTA AAGGAACAGG AGACCACTGG CATGCTGCAG 
GCCCAGCTTC AGGAAACACA GCAGGAGCTG AAGGAGGCAG CCCAGCAGCA CAGGGACGAC 
CTTGCTGCCT TCCAGAAGGA CAAGCTGGAC CTGCAGAAGC AGGTAGAGGA CCTAATGTCT 
CAGCTGGTAG CCCACGATGA CTCCCAGAGG CTGGTGAAAG AAGAGATTGA AGAGAAGGTG 
AAAGTGGCTC AAGAATGCAG CCGGATTCAG AAGGAACTGG AGAAAGAGAA CGCCAGCCTG 
GCTCTGTCGC TGGTGGAAAA GGAAAAACGA CTTCTCATTT TACAAGAAGC TGACTCTGTA 
AGACAGCAGG AGCTGAGCTC CTTGCGCCAG GACATACAGG AGGCCCAGGA AGGGCAAAGA 
GAGCTGGGTG TACAGGTGGA ACTATTGAGG CAGGAGGTGA AGGAAAAGGA GGCTGACTTT 
GTGGCCCGGG AAGCACAGCT GCTGGAGGAG TTGGAGGCCT CACGGGTTGC AGAGCAGCAA 
CTTCGAGCGT CTTTGTGGGC CCAGGAAGCC AAGGCAACCC AGCTGCAGCT GCAGCTGCGC 
AGCACTGAGA GCCAGCTAGA GGCATTGGTT GCAGAACAAC AGCCGGAGAA CCAGGCACAG 
GCACAGCTAG CTAGCCTCTG CTCTGTTCTG CAGCAGGCCC TGGGGTCTGC ATGTGAGAGT 
AGGCCTGAGC TGAGGGGTGG AGGCGACTCT GCTCCTACCT TATGGGGCCC TGATCCAGAT 
CAGAATGGAG CTTCCAGACT CTTTAAGAGA TGGTCGCTCC CAACGGCACT GTCACCGGAG 
GCTGTGGCAC TAGCTCTCCA GAAGCTCCAT CAGGACGTGT GGAAGGCTCG GCAGGCCCGG 
GATGACCTAA GGGACCAGGT CCAGAAGCTG GTGCAGCGCC TGACTGACAC GGAGGCCCAG 
AAGAGCCAGG TTCACTCGGA GCTGCAGGAC CTGCAGAGAC AGCTCTCTCA GAGCCAGGAA 
GAGAAATCCA AGTGGGAAGG GAGGCAGAAC TCCCTGGAGT CTGAGCTGAG GGACTTGCAC 
GAGACCGCGG CATCCTTACA GAGTCGCCTC CGGCAGGCAG AACTGCAAAA AATGGAAGCT 
CAGAATGATC GAGAGTTGCT TCAGGCATCC AAGGAGAAGC TGTCAGCCCA GGTTGAACAT 
CTGCAAGCAT GTGTTGCAGA AGCCCAGGCT CAGGCAGATG CAGCTGCTGT CCTGGAAGAA 
GATCTGCGAA CTGCTCGCTC TGCACTGAAA CTGAAAAACG AGGAGCTAGA GAGTGAACGG 
GAGAGAGCCC AGGCTTTACA AGAACAGGGT GAGCTGAAGG TGGCTCAGGG GAAAGCTTTG 
CAGGAGAACC TAGCCCTGCT GGCACAGACC CTGTCTAACA GAGAGCGGGA GGTCGAGACG 
TTGCAGGCTG AGGTTCAGGA ACTGGAGAAG CAGCGGGAGA TGCAGAAGGC TGCTCTGGAG 
CTGCTCTCCC TCGACCTGAA GAAGCGCAGT CGGGAGGTAG ACCTGCAACA AGAACAGATT 
CAGGAGCTGG AGCAGTGCAG GTCAGTCTTA GAGCACTTGC CCATGGCCGT CCAAGAGCGA 
GAGCAGAAAC TGAGTGTGCA GCGGGACCAG ATCAGGGAAC TGGAGAACGA TCGGGAGGCC 
CAGAGGAGTG TCCTGGAACA CCAGCTTCTG GACCTGGAGC AGAAGGCTCA AGTGATTGAG 
TCCCAGAGGG GACAGATTCA GGATCTGAAA AAGCAACTGG GGACACTGGA ATGCCTGGCC 
CTGGAACTGG AGGAAAGCCA TCACAAAGTG GAGAGCCAAC AGAAGATGAT CACAGAACTA 
GAGGGCCAGA GGGAAATGCA GAGGGTGGCT CTAACCCACC TCACACTGGA CCTGGAAGAA 
AGGAGCCAGG AGCTGCAGGC ACAAAGCAGC CAGCTCCATG AGCTGGAGAA CCATAGCACC 
CATCTGGCAA AAGAGCTGCA GGAGAGGGAC CAGGAGGTGA CGTCCCAACG CCAGCAGATC 
GATGAGCTGC AGAAGCAGCA GGAACAGCTG GCTCAGGCCC TGGAGAGGAA AGGCCAGGAG 
CTGGTGCTGC AGAAGGAAAG GATTCAGGTT CTGGAAGATC AGAGGACGCT GCAGACCAAG 
ATCTTAGAGG AGGACCTAGA ACAGATCAAG CATTCCTTGA GAGAACGCAG CCAGGAGCTG 
GCCTCTCAGT GGCAGCTGGT GCATGAGCGT GCCGACGATG GAAAGAGCCC GAGTAAAGGC 
CAGCGAGGGA GCCTGGAGCA CTTGAAGCTG ATCCTGCGTG ATAAGGAGAA GGAGGTAGAG 
TGCCAGCAGG AGCGCATCCA GGAACTTCAG GGCCACATGG GCCAGCTGGA ACAGCAGCTG 
CAGGGTCTGC ACAGGAAGGT GGGGGAGACT AGCCTGCTCC TGACCCATCG AGAGCAGGAG 
ACAGCAACCC TGCAGCAGCA TCTCCAGGAA GCCAAGGAAC AAGGGGAACT GAGAGAGCAG 
GTACTTCAGG GTCAGCTGGA GGAGGCCCAG AGAGATCTGG CCCAGAGGGA CCATGAACTA 
GAGACCCTCC GGCAGGAAAA ACAGCAGACC CAGGACCAGG AGGAAAGCAT GAAGCTAAAG 
ACAAGTGCTC TGCAGGCCGC TCTGGAGCAG GCCCATGCGA CACTGAAGGA GCGTCAGGGG 
GAGCTGGAAG AACACAGGGA ACAGGTGCGA AGGCTCCAGG AAGAACTAGA AGTGGAGGGA 
CGGCAGGTAC GGGCCCTGGA GGAGGTGTTG GGGGACCTAA GGGCTGAGTC TCGGGAGCAC 
GAGAAGGCTG TGCTGGCCCT TCAACAACGG TGTGCTGAGC AGGCCCAGGA ACATGAGGCA 
GAAGCCAGGA CCCTGCAGGA CAGCTGGCTG CAGGCCCAGG CCACTCTCAC AGAACAAGAA 
CAAGAGCTAG CGGCACTGCG AGCGGAAAAC CAGTATTCCC GCCGACAGGA AGAGGCTGCA 
GTTAGCCAGG CAGAGGCGCT GCAGGAGGCC CTCAGCAAAG CCCAGGCTGC CCTGCAGGAG 
AAAGAGCAGA GTCTGCTTGA ACAGGCTGAG TTGAGCCACA CCCTGGAGGC CAGCACTGCC 
GCCTTGCAAG CTACCCTGGA CACCTGCCAG GCAAGTGCCC GGCAGCTGGA GGAGGCCCTG 
AGGATTCGGG AAGGTGAGAT CCAGGCCCAG GCTCTCCAAC ACCACGAGGT CACGCAGCAC 
CTCCAGCAGG AACTTTGCCA GAAGAAAGAA GAGCTGAGGC AGCTACTAGA GAAGGCTGGG 
GCCCGGAGGA GTCAAGAAAA TGGAATCCAA GAGAAGCAAA GTCTGGAGCA AGAGAGACAA 
GAGGAAACAA GGAGGCTCCT TGAGAGCCTA AAGGAGCTAC AGCTGACTGT AGCCCAAAGG 
GAAGAAGAGA TTCTGATGCT GAGGGAGGCC TCGTCCCCAC GCCACAGAGC CCTCCCAGCC 
GAGAAGCCAG CTCTGCAACC GCTCCCAGCA CAACAGGAGT TAGAGCGGCT GCAGACTGCC 
CTAAGGCAAA CAGAGGCCAG GGAGATTGAA TGGAGGGAGA AGGCTCAGGA CCTGGCTCTG 
TCCCTGGCCC AAAGCAAGGC CAGCATCAGC AGTCTGCAGG AGATAACCAT GTTCCTACAA 
GCCTCTGTTC TAGAGCGGGA ATCAGAACAA CAAAGACTAC AGGAGGAGTT GGTACTCAGC 
AGACAAGCTC TGGAAGAGCA GCAGTCAGGT GGCCCACACT CAACCAGCAG AGCAGACCAG 
GGACCAAAAG TGGGACAGGG AAGCCAGTCT GGAGAGGTAG AGACTGAGCC TAGTCCTGGA 
GTGGAGGAGA AGGAACGGTT GACACAGAGG CTTGAACGTC TGCAGCAAGC GGTGGCAGAG 
CTGGAAGTTG ACCGAAGCAA GCTGCAGTGC CACAATGCGC AGCTGCGGAC CGCCTTGGAG 
CAGGTGGAAC GAGAACGGAG GAAGCTGAAG AGGGATTCCG TGCGTGCATC CCGGGCAGGA 
TCACTGGAGG CCAGAGAAAC TATGACTTCA TCCCCCACAC AGCAGGATGG GAGAGGCAGT 
CAGAGGGGTT CCTCGGACAG CGTGCTTGTG GTTGAGCTTC AGAGAGAGGT AGCCCTGCTG 
CGAGCCCAGC TGGCCTTGGA GCGAAAGCAG AGGCAGGACT ACATTGCACG GTCCGTGCAG 
ACCAGCCGTG AGCTAGCAGG CCTGCATCAC AGCCTGTCTC ACTCCCTCCT CACCGTGGCC 
CAGGCCCCTG AGGCCACTGT TCTGGAGGCT GAGACCCGAA AGCTGGATGA GTCTCTGAAT 
CAGAGTCTGA CATCCCCAGG GCCATGTTTG CTACATCCCA GTCTGGACAC TACTCAAAAT 
ACCCACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103526.1
CDS	930..7661
Translation	MKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	XM_030247666.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247666.1

ORF Insert Sequence:

ATGAAGTCAG CTACTGACAG AGACCTGACA GAGCTAAAGG CTGAACACGC AAGGCTTTCA 
GGGTCCCTGC TGACCTGCTG TCTGCGCCTG ACCCTGAGAG CACAGTCTCG GGAGTCCAGT 
GGATCTGGGA GAACAGAGGA GAGTGAGCCA GCCCGGCTGC TGCTGCTCGT CGCTAAGACC 
CAGGCGCTGG AGAAGGAAGC CCATGAGAAG AGCCAGGAGT TAATGCAGCT CAAGAGTCAC 
GGGGATCTAG AGAAGGCAGA GCTGCAGGAC CGAGTGACGG AACTCTCTGC TCTGCTAACC 
CAGTCTCAGA AGCAAAATGA AGATTATGAG AAGATGGTAA AGGCTCTAAG AGAAACAATG 
GAGATCCTGG AGACAAACCA TGCAGAGTTA ATGGAGCATG AGGCATCTCT CAGTAGGAAT 
GCTCAAGAGG AAAAGTTGTC TCTGCAGCAG GTGATCAAGG CTATAACCCA GGCACTGGCC 
TCAGTGGAAG AAGAAGACAC TGTGACCCAG TCCTCAGGCC ATGAGGACTC GCTGCAGTCA 
GACTGTAATG GCCTTTCCCA GTTTGATCCC CAGGACCCAG ACAGGGCTCT TACCCTGGTG 
CAGTCAGTGC TGACTCGGAG ACAGCAGGCT GTGCAGGACC TCAGGCAGCA GCTTTCAGGC 
TGCCAGGAGG CTATGAGCTT CTTGCAGCAG CAGCACGATC AGTGGGAGGA AGAGGGCAGG 
GCCCTGAGGG AAAAGCTACA GAAGCTCACT GGGGAGCGGG ATGCTCTGGC AGGCCAGACT 
GTGGGCCTTC AGGGAGAGGT GGACTCTCTC ACTGGGGAGC GGGATGCTCT GGCAGGCCAG 
ACTGTGGACC TTCAGGGAGA GGTGGACTCT CTCAGCAGGG AGCGGGAGCT GCTTCAGAAG 
GCCAGAGGAG AGCTGCAGCA GCAACTGGAA GTGCTGGAGC AGGAAGCGTG GCGACTGCGG 
AGGATGAACA TGGAGCTGCA GCTGCAGGGG GACTCAGCTC AAGGGGAGAA GCTGGAGCAG 
CAGGAGGAGT TGCACTTGGC TGTCCGGGAG AGGGAGCGCC TTCAGGAGAC GCTGGTGGGC 
CTGGAAGCCA AGCAGTCAGA ATCACTAAGT GAGCTGCTCA CTCTTCGAGA AGCCCTGGAG 
TCAAGTCGCC TAGAAGGGGA GTTACTGAAG CAGGAGCGAG TGGAAGTGGC TGCAGCCTTG 
GCCAGGGCAG AACAGTCCAT TGTGGAGCTG TCAGGTTCTG AGAACAGCCT GAAGGCAGAG 
GTAGCTGATC TTCGAGCTGC AGCTGTCAAG CTTGGGGCCT TAAACGAGGC TTTGGCTTTA 
GATAAAGTTG AACTGAACCA GCAGCTTCTC CAGTTGGAGC AGGAGAACCA GTCTCTATGC 
AGCAGAGTGG AGGCCGCAGA GCAGCTGAGA AGCGCTTTGC AGGTGGACCT GGCGGAGGCA 
GAAAGGCGCA GGGAAGCCCT GTGGGAGAAG AAAACTCAGC TGGAGACTCA GCTGCAGAAG 
GCAGAGGAAG CGGGGGCTGA GCTACAGGCA GAGCTGAGGG GCACCCGAGA AGAAAAGGAA 
GAGCTTAAAG ATAAATTAAG TGAGGCACAT CACCAGCAAG AAACAGCTAC AGCTCACCTA 
GAACAGCTGC ATCAGGACGC AGAAAGACAG GAAGAAACAC TTGCTAGGGC AGTCCAGGAG 
AAGGAAGCCC TAGTACGGGA GAGGGCAGCT CTTGAGGTGC GCCTGCAGGC TGTGGAGCGG 
GACCGACAGG ACCTCACTGA ACATGTCCTG GGGCTCAGGT CAGCCAAGGA GCAACTGGAG 
AGCAATCTAT TTGAGGCCCA ACAGCAAAAT TCTGTGATAC AGGTCACCAA GGGGCAGCTG 
GAGGTCCAGA TTCAAACTAT TATCCAAGCC AAGGAAGTAA TTCAAGGGGA AGTGAAATGC 
CTGAAGCTGG AACTGGATGC TGAGAGGACC CGGGCAGAGC AGGAGTGGGA TGCAGTAGCC 
AGGCAGCTAG CCCAAGCTGA GCAGGAGGGG CAAGCATCTC TGGAGCGACA GAAGGTGGCC 
CACGAGGAGG AGGTGAACAG GCTGCAAGAG AAATGGGAGA AAGAGCGCTC CTGGCTGCAG 
CAGGAGCTGG ATAAGACCCT GGAAACCCTC GAGAGGGAGA GAGCAGAGCT GGAGACAAAG 
CTGAGAGAGC AGCAGACAGA AATGGAAGCC ATCCGTGCAC AGAGGGAAGA GGAACGAAGC 
CAGGCTGACA GCGCCCTGTA TCAAATGCAG CTAGAAACAG AGAAGGAGAG AGTATCCCTC 
CTGGAGACAC TGCTGAGGAC CCAGAAGGAG CTGGCAGATG CTAGTCAACA GCTGGAAAGG 
CTGAGACAGG ACATGAAGAT TCAGAAGTTA AAGGAACAGG AGACCACTGG CATGCTGCAG 
GCCCAGCTTC AGGAAACACA GCAGGAGCTG AAGGAGGCAG CCCAGCAGCA CAGGGACGAC 
CTTGCTGCCT TCCAGAAGGA CAAGCTGGAC CTGCAGAAGC AGGTAGAGGA CCTAATGTCT 
CAGCTGGTAG CCCACGATGA CTCCCAGAGG CTGGTGAAAG AAGAGATTGA AGAGAAGGTG 
AAAGTGGCTC AAGAATGCAG CCGGATTCAG AAGGAACTGG AGAAAGAGAA CGCCAGCCTG 
GCTCTGTCGC TGGTGGAAAA GGAAAAACGA CTTCTCATTT TACAAGAAGC TGACTCTGTA 
AGACAGCAGG AGCTGAGCTC CTTGCGCCAG GACATACAGG AGGCCCAGGA AGGGCAAAGA 
GAGCTGGGTG TACAGGTGGA ACTATTGAGG CAGGAGGTGA AGGAAAAGGA GGCTGACTTT 
GTGGCCCGGG AAGCACAGCT GCTGGAGGAG TTGGAGGCCT CACGGGTTGC AGAGCAGCAA 
CTTCGAGCGT CTTTGTGGGC CCAGGAAGCC AAGGCAACCC AGCTGCAGCT GCAGCTGCGC 
AGCACTGAGA GCCAGCTAGA GGCATTGGTT GCAGAACAAC AGCCGGAGAA CCAGGCACAG 
GCACAGCTAG CTAGCCTCTG CTCTGTTCTG CAGCAGGCCC TGGGGTCTGC ATGTGAGAGT 
AGGCCTGAGC TGAGGGGTGG AGGCGACTCT GCTCCTACCT TATGGGGCCC TGATCCAGAT 
CAGAATGGAG CTTCCAGACT CTTTAAGAGA TGGTCGCTCC CAACGGCACT GTCACCGGAG 
GCTGTGGCAC TAGCTCTCCA GAAGCTCCAT CAGGACGTGT GGAAGGCTCG GCAGGCCCGG 
GATGACCTAA GGGACCAGGT CCAGAAGCTG GTGCAGCGCC TGACTGACAC GGAGGCCCAG 
AAGAGCCAGG TTCACTCGGA GCTGCAGGAC CTGCAGAGAC AGCTCTCTCA GAGCCAGGAA 
GAGAAATCCA AGTGGGAAGG GAGGCAGAAC TCCCTGGAGT CTGAGCTGAG GGACTTGCAC 
GAGACCGCGG CATCCTTACA GAGTCGCCTC CGGCAGGCAG AACTGCAAAA AATGGAAGCT 
CAGAATGATC GAGAGTTGCT TCAGGCATCC AAGGAGAAGC TGTCAGCCCA GGTTGAACAT 
CTGCAAGCAT GTGTTGCAGA AGCCCAGGCT CAGGCAGATG CAGCTGCTGT CCTGGAAGAA 
GATCTGCGAA CTGCTCGCTC TGCACTGAAA CTGAAAAACG AGGAGCTAGA GAGTGAACGG 
GAGAGAGCCC AGGCTTTACA AGAACAGGGT GAGCTGAAGG TGGCTCAGGG GAAAGCTTTG 
CAGGAGAACC TAGCCCTGCT GGCACAGACC CTGTCTAACA GAGAGCGGGA GGTCGAGACG 
TTGCAGGCTG AGGTTCAGGA ACTGGAGAAG CAGCGGGAGA TGCAGAAGGC TGCTCTGGAG 
CTGCTCTCCC TCGACCTGAA GAAGCGCAGT CGGGAGGTAG ACCTGCAACA AGAACAGATT 
CAGGAGCTGG AGCAGTGCAG GTCAGTCTTA GAGCACTTGC CCATGGCCGT CCAAGAGCGA 
GAGCAGAAAC TGAGTGTGCA GCGGGACCAG ATCAGGGAAC TGGAGAACGA TCGGGAGGCC 
CAGAGGAGTG TCCTGGAACA CCAGCTTCTG GACCTGGAGC AGAAGGCTCA AGTGATTGAG 
TCCCAGAGGG GACAGATTCA GGATCTGAAA AAGCAACTGG GGACACTGGA ATGCCTGGCC 
CTGGAACTGG AGGAAAGCCA TCACAAAGTG GAGAGCCAAC AGAAGATGAT CACAGAACTA 
GAGGGCCAGA GGGAAATGCA GAGGGTGGCT CTAACCCACC TCACACTGGA CCTGGAAGAA 
AGGAGCCAGG AGCTGCAGGC ACAAAGCAGC CAGCTCCATG AGCTGGAGAA CCATAGCACC 
CATCTGGCAA AAGAGCTGCA GGAGAGGGAC CAGGAGGTGA CGTCCCAACG CCAGCAGATC 
GATGAGCTGC AGAAGCAGCA GGAACAGCTG GCTCAGGCCC TGGAGAGGAA AGGCCAGGAG 
CTGGTGCTGC AGAAGGAAAG GATTCAGGTT CTGGAAGATC AGAGGACGCT GCAGACCAAG 
ATCTTAGAGG AGGACCTAGA ACAGATCAAG CATTCCTTGA GAGAACGCAG CCAGGAGCTG 
GCCTCTCAGT GGCAGCTGGT GCATGAGCGT GCCGACGATG GAAAGAGCCC GAGTAAAGGC 
CAGCGAGGGA GCCTGGAGCA CTTGAAGCTG ATCCTGCGTG ATAAGGAGAA GGAGGTAGAG 
TGCCAGCAGG AGCGCATCCA GGAACTTCAG GGCCACATGG GCCAGCTGGA ACAGCAGCTG 
CAGGGTCTGC ACAGGAAGGT GGGGGAGACT AGCCTGCTCC TGACCCATCG AGAGCAGGAG 
ACAGCAACCC TGCAGCAGCA TCTCCAGGAA GCCAAGGAAC AAGGGGAACT GAGAGAGCAG 
GTACTTCAGG GTCAGCTGGA GGAGGCCCAG AGAGATCTGG CCCAGAGGGA CCATGAACTA 
GAGACCCTCC GGCAGGAAAA ACAGCAGACC CAGGACCAGG AGGAAAGCAT GAAGCTAAAG 
ACAAGTGCTC TGCAGGCCGC TCTGGAGCAG GCCCATGCGA CACTGAAGGA GCGTCAGGGG 
GAGCTGGAAG AACACAGGGA ACAGGTGCGA AGGCTCCAGG AAGAACTAGA AGTGGAGGGA 
CGGCAGGTAC GGGCCCTGGA GGAGGTGTTG GGGGACCTAA GGGCTGAGTC TCGGGAGCAC 
GAGAAGGCTG TGCTGGCCCT TCAACAACGG TGTGCTGAGC AGGCCCAGGA ACATGAGGCA 
GAAGCCAGGA CCCTGCAGGA CAGCTGGCTG CAGGCCCAGG CCACTCTCAC AGAACAAGAA 
CAAGAGCTAG CGGCACTGCG AGCGGAAAAC CAGTATTCCC GCCGACAGGA AGAGGCTGCA 
GTTAGCCAGG CAGAGGCGCT GCAGGAGGCC CTCAGCAAAG CCCAGGCTGC CCTGCAGGAG 
AAAGAGCAGA GTCTGCTTGA ACAGGCTGAG TTGAGCCACA CCCTGGAGGC CAGCACTGCC 
GCCTTGCAAG CTACCCTGGA CACCTGCCAG GCAAGTGCCC GGCAGCTGGA GGAGGCCCTG 
AGGATTCGGG AAGGTGAGAT CCAGGCCCAG GCTCTCCAAC ACCACGAGGT CACGCAGCAC 
CTCCAGCAGG AACTTTGCCA GAAGAAAGAA GAGCTGAGGC AGCTACTAGA GAAGGCTGGG 
GCCCGGAGGA GTCAAGAAAA TGGAATCCAA GAGAAGCAAA GTCTGGAGCA AGAGAGACAA 
GAGGAAACAA GGAGGCTCCT TGAGAGCCTA AAGGAGCTAC AGCTGACTGT AGCCCAAAGG 
GAAGAAGAGA TTCTGATGCT GAGGGAGGCC TCGTCCCCAC GCCACAGAGC CCTCCCAGCC 
GAGAAGCCAG CTCTGCAACC GCTCCCAGCA CAACAGGAGT TAGAGCGGCT GCAGACTGCC 
CTAAGGCAAA CAGAGGCCAG GGAGATTGAA TGGAGGGAGA AGGCTCAGGA CCTGGCTCTG 
TCCCTGGCCC AAAGCAAGGC CAGCATCAGC AGTCTGCAGG AGATAACCAT GTTCCTACAA 
GCCTCTGTTC TAGAGCGGGA ATCAGAACAA CAAAGACTAC AGGAGGAGTT GGTACTCAGC 
AGACAAGCTC TGGAAGAGCA GCAGTCAGGT GGCCCACACT CAACCAGCAG AGCAGACCAG 
GGACCAAAAG TGGGACAGGG AAGCCAGTCT GGAGAGGTAG AGACTGAGCC TAGTCCTGGA 
GTGGAGGAGA AGGAACGGTT GACACAGAGG CTTGAACGTC TGCAGCAAGC GGTGGCAGAG 
CTGGAAGTTG ACCGAAGCAA GCTGCAGTGC CACAATGCGC AGCTGCGGAC CGCCTTGGAG 
CAGGTGGAAC GAGAACGGAG GAAGCTGAAG AGGGATTCCG TGCGTGCATC CCGGGCAGGA 
TCACTGGAGG CCAGAGAAAC TATGACTTCA TCCCCCACAC AGCAGGATGG GAGAGGCAGT 
CAGAGGGGTT CCTCGGACAG CGTGCTTGTG GTTGAGCTTC AGAGAGAGGT AGCCCTGCTG 
CGAGCCCAGC TGGCCTTGGA GCGAAAGCAG AGGCAGGACT ACATTGCACG GTCCGTGCAG 
ACCAGCCGTG AGCTAGCAGG CCTGCATCAC AGCCTGTCTC ACTCCCTCCT CACCGTGGCC 
CAGGCCCCTG AGGCCACTGT TCTGGAGGCT GAGACCCGAA AGCTGGATGA GTCTCTGAAT 
CAGAGTCTGA CATCCCCAGG GCCATGTTTG CTACATCCCA GTCTGGACAC TACTCAAAAT 
ACCCACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( XM_030247667.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103527.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant X7, mRNA.

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CloneID	OMu87726
Clone ID Related Accession (Same CDS sequence)	XM_030247663.1 , XM_030247666.1 , XM_030247667.1
Accession Version	XM_030247667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAG CTACTGACAG AGACCTGACA GAGCTAAAGG CTGAACACGC AAGGCTTTCA 
GGGTCCCTGC TGACCTGCTG TCTGCGCCTG ACCCTGAGAG CACAGTCTCG GGAGTCCAGT 
GGATCTGGGA GAACAGAGGA GAGTGAGCCA GCCCGGCTGC TGCTGCTCGT CGCTAAGACC 
CAGGCGCTGG AGAAGGAAGC CCATGAGAAG AGCCAGGAGT TAATGCAGCT CAAGAGTCAC 
GGGGATCTAG AGAAGGCAGA GCTGCAGGAC CGAGTGACGG AACTCTCTGC TCTGCTAACC 
CAGTCTCAGA AGCAAAATGA AGATTATGAG AAGATGGTAA AGGCTCTAAG AGAAACAATG 
GAGATCCTGG AGACAAACCA TGCAGAGTTA ATGGAGCATG AGGCATCTCT CAGTAGGAAT 
GCTCAAGAGG AAAAGTTGTC TCTGCAGCAG GTGATCAAGG CTATAACCCA GGCACTGGCC 
TCAGTGGAAG AAGAAGACAC TGTGACCCAG TCCTCAGGCC ATGAGGACTC GCTGCAGTCA 
GACTGTAATG GCCTTTCCCA GTTTGATCCC CAGGACCCAG ACAGGGCTCT TACCCTGGTG 
CAGTCAGTGC TGACTCGGAG ACAGCAGGCT GTGCAGGACC TCAGGCAGCA GCTTTCAGGC 
TGCCAGGAGG CTATGAGCTT CTTGCAGCAG CAGCACGATC AGTGGGAGGA AGAGGGCAGG 
GCCCTGAGGG AAAAGCTACA GAAGCTCACT GGGGAGCGGG ATGCTCTGGC AGGCCAGACT 
GTGGGCCTTC AGGGAGAGGT GGACTCTCTC ACTGGGGAGC GGGATGCTCT GGCAGGCCAG 
ACTGTGGACC TTCAGGGAGA GGTGGACTCT CTCAGCAGGG AGCGGGAGCT GCTTCAGAAG 
GCCAGAGGAG AGCTGCAGCA GCAACTGGAA GTGCTGGAGC AGGAAGCGTG GCGACTGCGG 
AGGATGAACA TGGAGCTGCA GCTGCAGGGG GACTCAGCTC AAGGGGAGAA GCTGGAGCAG 
CAGGAGGAGT TGCACTTGGC TGTCCGGGAG AGGGAGCGCC TTCAGGAGAC GCTGGTGGGC 
CTGGAAGCCA AGCAGTCAGA ATCACTAAGT GAGCTGCTCA CTCTTCGAGA AGCCCTGGAG 
TCAAGTCGCC TAGAAGGGGA GTTACTGAAG CAGGAGCGAG TGGAAGTGGC TGCAGCCTTG 
GCCAGGGCAG AACAGTCCAT TGTGGAGCTG TCAGGTTCTG AGAACAGCCT GAAGGCAGAG 
GTAGCTGATC TTCGAGCTGC AGCTGTCAAG CTTGGGGCCT TAAACGAGGC TTTGGCTTTA 
GATAAAGTTG AACTGAACCA GCAGCTTCTC CAGTTGGAGC AGGAGAACCA GTCTCTATGC 
AGCAGAGTGG AGGCCGCAGA GCAGCTGAGA AGCGCTTTGC AGGTGGACCT GGCGGAGGCA 
GAAAGGCGCA GGGAAGCCCT GTGGGAGAAG AAAACTCAGC TGGAGACTCA GCTGCAGAAG 
GCAGAGGAAG CGGGGGCTGA GCTACAGGCA GAGCTGAGGG GCACCCGAGA AGAAAAGGAA 
GAGCTTAAAG ATAAATTAAG TGAGGCACAT CACCAGCAAG AAACAGCTAC AGCTCACCTA 
GAACAGCTGC ATCAGGACGC AGAAAGACAG GAAGAAACAC TTGCTAGGGC AGTCCAGGAG 
AAGGAAGCCC TAGTACGGGA GAGGGCAGCT CTTGAGGTGC GCCTGCAGGC TGTGGAGCGG 
GACCGACAGG ACCTCACTGA ACATGTCCTG GGGCTCAGGT CAGCCAAGGA GCAACTGGAG 
AGCAATCTAT TTGAGGCCCA ACAGCAAAAT TCTGTGATAC AGGTCACCAA GGGGCAGCTG 
GAGGTCCAGA TTCAAACTAT TATCCAAGCC AAGGAAGTAA TTCAAGGGGA AGTGAAATGC 
CTGAAGCTGG AACTGGATGC TGAGAGGACC CGGGCAGAGC AGGAGTGGGA TGCAGTAGCC 
AGGCAGCTAG CCCAAGCTGA GCAGGAGGGG CAAGCATCTC TGGAGCGACA GAAGGTGGCC 
CACGAGGAGG AGGTGAACAG GCTGCAAGAG AAATGGGAGA AAGAGCGCTC CTGGCTGCAG 
CAGGAGCTGG ATAAGACCCT GGAAACCCTC GAGAGGGAGA GAGCAGAGCT GGAGACAAAG 
CTGAGAGAGC AGCAGACAGA AATGGAAGCC ATCCGTGCAC AGAGGGAAGA GGAACGAAGC 
CAGGCTGACA GCGCCCTGTA TCAAATGCAG CTAGAAACAG AGAAGGAGAG AGTATCCCTC 
CTGGAGACAC TGCTGAGGAC CCAGAAGGAG CTGGCAGATG CTAGTCAACA GCTGGAAAGG 
CTGAGACAGG ACATGAAGAT TCAGAAGTTA AAGGAACAGG AGACCACTGG CATGCTGCAG 
GCCCAGCTTC AGGAAACACA GCAGGAGCTG AAGGAGGCAG CCCAGCAGCA CAGGGACGAC 
CTTGCTGCCT TCCAGAAGGA CAAGCTGGAC CTGCAGAAGC AGGTAGAGGA CCTAATGTCT 
CAGCTGGTAG CCCACGATGA CTCCCAGAGG CTGGTGAAAG AAGAGATTGA AGAGAAGGTG 
AAAGTGGCTC AAGAATGCAG CCGGATTCAG AAGGAACTGG AGAAAGAGAA CGCCAGCCTG 
GCTCTGTCGC TGGTGGAAAA GGAAAAACGA CTTCTCATTT TACAAGAAGC TGACTCTGTA 
AGACAGCAGG AGCTGAGCTC CTTGCGCCAG GACATACAGG AGGCCCAGGA AGGGCAAAGA 
GAGCTGGGTG TACAGGTGGA ACTATTGAGG CAGGAGGTGA AGGAAAAGGA GGCTGACTTT 
GTGGCCCGGG AAGCACAGCT GCTGGAGGAG TTGGAGGCCT CACGGGTTGC AGAGCAGCAA 
CTTCGAGCGT CTTTGTGGGC CCAGGAAGCC AAGGCAACCC AGCTGCAGCT GCAGCTGCGC 
AGCACTGAGA GCCAGCTAGA GGCATTGGTT GCAGAACAAC AGCCGGAGAA CCAGGCACAG 
GCACAGCTAG CTAGCCTCTG CTCTGTTCTG CAGCAGGCCC TGGGGTCTGC ATGTGAGAGT 
AGGCCTGAGC TGAGGGGTGG AGGCGACTCT GCTCCTACCT TATGGGGCCC TGATCCAGAT 
CAGAATGGAG CTTCCAGACT CTTTAAGAGA TGGTCGCTCC CAACGGCACT GTCACCGGAG 
GCTGTGGCAC TAGCTCTCCA GAAGCTCCAT CAGGACGTGT GGAAGGCTCG GCAGGCCCGG 
GATGACCTAA GGGACCAGGT CCAGAAGCTG GTGCAGCGCC TGACTGACAC GGAGGCCCAG 
AAGAGCCAGG TTCACTCGGA GCTGCAGGAC CTGCAGAGAC AGCTCTCTCA GAGCCAGGAA 
GAGAAATCCA AGTGGGAAGG GAGGCAGAAC TCCCTGGAGT CTGAGCTGAG GGACTTGCAC 
GAGACCGCGG CATCCTTACA GAGTCGCCTC CGGCAGGCAG AACTGCAAAA AATGGAAGCT 
CAGAATGATC GAGAGTTGCT TCAGGCATCC AAGGAGAAGC TGTCAGCCCA GGTTGAACAT 
CTGCAAGCAT GTGTTGCAGA AGCCCAGGCT CAGGCAGATG CAGCTGCTGT CCTGGAAGAA 
GATCTGCGAA CTGCTCGCTC TGCACTGAAA CTGAAAAACG AGGAGCTAGA GAGTGAACGG 
GAGAGAGCCC AGGCTTTACA AGAACAGGGT GAGCTGAAGG TGGCTCAGGG GAAAGCTTTG 
CAGGAGAACC TAGCCCTGCT GGCACAGACC CTGTCTAACA GAGAGCGGGA GGTCGAGACG 
TTGCAGGCTG AGGTTCAGGA ACTGGAGAAG CAGCGGGAGA TGCAGAAGGC TGCTCTGGAG 
CTGCTCTCCC TCGACCTGAA GAAGCGCAGT CGGGAGGTAG ACCTGCAACA AGAACAGATT 
CAGGAGCTGG AGCAGTGCAG GTCAGTCTTA GAGCACTTGC CCATGGCCGT CCAAGAGCGA 
GAGCAGAAAC TGAGTGTGCA GCGGGACCAG ATCAGGGAAC TGGAGAACGA TCGGGAGGCC 
CAGAGGAGTG TCCTGGAACA CCAGCTTCTG GACCTGGAGC AGAAGGCTCA AGTGATTGAG 
TCCCAGAGGG GACAGATTCA GGATCTGAAA AAGCAACTGG GGACACTGGA ATGCCTGGCC 
CTGGAACTGG AGGAAAGCCA TCACAAAGTG GAGAGCCAAC AGAAGATGAT CACAGAACTA 
GAGGGCCAGA GGGAAATGCA GAGGGTGGCT CTAACCCACC TCACACTGGA CCTGGAAGAA 
AGGAGCCAGG AGCTGCAGGC ACAAAGCAGC CAGCTCCATG AGCTGGAGAA CCATAGCACC 
CATCTGGCAA AAGAGCTGCA GGAGAGGGAC CAGGAGGTGA CGTCCCAACG CCAGCAGATC 
GATGAGCTGC AGAAGCAGCA GGAACAGCTG GCTCAGGCCC TGGAGAGGAA AGGCCAGGAG 
CTGGTGCTGC AGAAGGAAAG GATTCAGGTT CTGGAAGATC AGAGGACGCT GCAGACCAAG 
ATCTTAGAGG AGGACCTAGA ACAGATCAAG CATTCCTTGA GAGAACGCAG CCAGGAGCTG 
GCCTCTCAGT GGCAGCTGGT GCATGAGCGT GCCGACGATG GAAAGAGCCC GAGTAAAGGC 
CAGCGAGGGA GCCTGGAGCA CTTGAAGCTG ATCCTGCGTG ATAAGGAGAA GGAGGTAGAG 
TGCCAGCAGG AGCGCATCCA GGAACTTCAG GGCCACATGG GCCAGCTGGA ACAGCAGCTG 
CAGGGTCTGC ACAGGAAGGT GGGGGAGACT AGCCTGCTCC TGACCCATCG AGAGCAGGAG 
ACAGCAACCC TGCAGCAGCA TCTCCAGGAA GCCAAGGAAC AAGGGGAACT GAGAGAGCAG 
GTACTTCAGG GTCAGCTGGA GGAGGCCCAG AGAGATCTGG CCCAGAGGGA CCATGAACTA 
GAGACCCTCC GGCAGGAAAA ACAGCAGACC CAGGACCAGG AGGAAAGCAT GAAGCTAAAG 
ACAAGTGCTC TGCAGGCCGC TCTGGAGCAG GCCCATGCGA CACTGAAGGA GCGTCAGGGG 
GAGCTGGAAG AACACAGGGA ACAGGTGCGA AGGCTCCAGG AAGAACTAGA AGTGGAGGGA 
CGGCAGGTAC GGGCCCTGGA GGAGGTGTTG GGGGACCTAA GGGCTGAGTC TCGGGAGCAC 
GAGAAGGCTG TGCTGGCCCT TCAACAACGG TGTGCTGAGC AGGCCCAGGA ACATGAGGCA 
GAAGCCAGGA CCCTGCAGGA CAGCTGGCTG CAGGCCCAGG CCACTCTCAC AGAACAAGAA 
CAAGAGCTAG CGGCACTGCG AGCGGAAAAC CAGTATTCCC GCCGACAGGA AGAGGCTGCA 
GTTAGCCAGG CAGAGGCGCT GCAGGAGGCC CTCAGCAAAG CCCAGGCTGC CCTGCAGGAG 
AAAGAGCAGA GTCTGCTTGA ACAGGCTGAG TTGAGCCACA CCCTGGAGGC CAGCACTGCC 
GCCTTGCAAG CTACCCTGGA CACCTGCCAG GCAAGTGCCC GGCAGCTGGA GGAGGCCCTG 
AGGATTCGGG AAGGTGAGAT CCAGGCCCAG GCTCTCCAAC ACCACGAGGT CACGCAGCAC 
CTCCAGCAGG AACTTTGCCA GAAGAAAGAA GAGCTGAGGC AGCTACTAGA GAAGGCTGGG 
GCCCGGAGGA GTCAAGAAAA TGGAATCCAA GAGAAGCAAA GTCTGGAGCA AGAGAGACAA 
GAGGAAACAA GGAGGCTCCT TGAGAGCCTA AAGGAGCTAC AGCTGACTGT AGCCCAAAGG 
GAAGAAGAGA TTCTGATGCT GAGGGAGGCC TCGTCCCCAC GCCACAGAGC CCTCCCAGCC 
GAGAAGCCAG CTCTGCAACC GCTCCCAGCA CAACAGGAGT TAGAGCGGCT GCAGACTGCC 
CTAAGGCAAA CAGAGGCCAG GGAGATTGAA TGGAGGGAGA AGGCTCAGGA CCTGGCTCTG 
TCCCTGGCCC AAAGCAAGGC CAGCATCAGC AGTCTGCAGG AGATAACCAT GTTCCTACAA 
GCCTCTGTTC TAGAGCGGGA ATCAGAACAA CAAAGACTAC AGGAGGAGTT GGTACTCAGC 
AGACAAGCTC TGGAAGAGCA GCAGTCAGGT GGCCCACACT CAACCAGCAG AGCAGACCAG 
GGACCAAAAG TGGGACAGGG AAGCCAGTCT GGAGAGGTAG AGACTGAGCC TAGTCCTGGA 
GTGGAGGAGA AGGAACGGTT GACACAGAGG CTTGAACGTC TGCAGCAAGC GGTGGCAGAG 
CTGGAAGTTG ACCGAAGCAA GCTGCAGTGC CACAATGCGC AGCTGCGGAC CGCCTTGGAG 
CAGGTGGAAC GAGAACGGAG GAAGCTGAAG AGGGATTCCG TGCGTGCATC CCGGGCAGGA 
TCACTGGAGG CCAGAGAAAC TATGACTTCA TCCCCCACAC AGCAGGATGG GAGAGGCAGT 
CAGAGGGGTT CCTCGGACAG CGTGCTTGTG GTTGAGCTTC AGAGAGAGGT AGCCCTGCTG 
CGAGCCCAGC TGGCCTTGGA GCGAAAGCAG AGGCAGGACT ACATTGCACG GTCCGTGCAG 
ACCAGCCGTG AGCTAGCAGG CCTGCATCAC AGCCTGTCTC ACTCCCTCCT CACCGTGGCC 
CAGGCCCCTG AGGCCACTGT TCTGGAGGCT GAGACCCGAA AGCTGGATGA GTCTCTGAAT 
CAGAGTCTGA CATCCCCAGG GCCATGTTTG CTACATCCCA GTCTGGACAC TACTCAAAAT 
ACCCACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103527.1
CDS	933..7664
Translation	MKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	XM_030247667.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247667.1

ORF Insert Sequence:

ATGAAGTCAG CTACTGACAG AGACCTGACA GAGCTAAAGG CTGAACACGC AAGGCTTTCA 
GGGTCCCTGC TGACCTGCTG TCTGCGCCTG ACCCTGAGAG CACAGTCTCG GGAGTCCAGT 
GGATCTGGGA GAACAGAGGA GAGTGAGCCA GCCCGGCTGC TGCTGCTCGT CGCTAAGACC 
CAGGCGCTGG AGAAGGAAGC CCATGAGAAG AGCCAGGAGT TAATGCAGCT CAAGAGTCAC 
GGGGATCTAG AGAAGGCAGA GCTGCAGGAC CGAGTGACGG AACTCTCTGC TCTGCTAACC 
CAGTCTCAGA AGCAAAATGA AGATTATGAG AAGATGGTAA AGGCTCTAAG AGAAACAATG 
GAGATCCTGG AGACAAACCA TGCAGAGTTA ATGGAGCATG AGGCATCTCT CAGTAGGAAT 
GCTCAAGAGG AAAAGTTGTC TCTGCAGCAG GTGATCAAGG CTATAACCCA GGCACTGGCC 
TCAGTGGAAG AAGAAGACAC TGTGACCCAG TCCTCAGGCC ATGAGGACTC GCTGCAGTCA 
GACTGTAATG GCCTTTCCCA GTTTGATCCC CAGGACCCAG ACAGGGCTCT TACCCTGGTG 
CAGTCAGTGC TGACTCGGAG ACAGCAGGCT GTGCAGGACC TCAGGCAGCA GCTTTCAGGC 
TGCCAGGAGG CTATGAGCTT CTTGCAGCAG CAGCACGATC AGTGGGAGGA AGAGGGCAGG 
GCCCTGAGGG AAAAGCTACA GAAGCTCACT GGGGAGCGGG ATGCTCTGGC AGGCCAGACT 
GTGGGCCTTC AGGGAGAGGT GGACTCTCTC ACTGGGGAGC GGGATGCTCT GGCAGGCCAG 
ACTGTGGACC TTCAGGGAGA GGTGGACTCT CTCAGCAGGG AGCGGGAGCT GCTTCAGAAG 
GCCAGAGGAG AGCTGCAGCA GCAACTGGAA GTGCTGGAGC AGGAAGCGTG GCGACTGCGG 
AGGATGAACA TGGAGCTGCA GCTGCAGGGG GACTCAGCTC AAGGGGAGAA GCTGGAGCAG 
CAGGAGGAGT TGCACTTGGC TGTCCGGGAG AGGGAGCGCC TTCAGGAGAC GCTGGTGGGC 
CTGGAAGCCA AGCAGTCAGA ATCACTAAGT GAGCTGCTCA CTCTTCGAGA AGCCCTGGAG 
TCAAGTCGCC TAGAAGGGGA GTTACTGAAG CAGGAGCGAG TGGAAGTGGC TGCAGCCTTG 
GCCAGGGCAG AACAGTCCAT TGTGGAGCTG TCAGGTTCTG AGAACAGCCT GAAGGCAGAG 
GTAGCTGATC TTCGAGCTGC AGCTGTCAAG CTTGGGGCCT TAAACGAGGC TTTGGCTTTA 
GATAAAGTTG AACTGAACCA GCAGCTTCTC CAGTTGGAGC AGGAGAACCA GTCTCTATGC 
AGCAGAGTGG AGGCCGCAGA GCAGCTGAGA AGCGCTTTGC AGGTGGACCT GGCGGAGGCA 
GAAAGGCGCA GGGAAGCCCT GTGGGAGAAG AAAACTCAGC TGGAGACTCA GCTGCAGAAG 
GCAGAGGAAG CGGGGGCTGA GCTACAGGCA GAGCTGAGGG GCACCCGAGA AGAAAAGGAA 
GAGCTTAAAG ATAAATTAAG TGAGGCACAT CACCAGCAAG AAACAGCTAC AGCTCACCTA 
GAACAGCTGC ATCAGGACGC AGAAAGACAG GAAGAAACAC TTGCTAGGGC AGTCCAGGAG 
AAGGAAGCCC TAGTACGGGA GAGGGCAGCT CTTGAGGTGC GCCTGCAGGC TGTGGAGCGG 
GACCGACAGG ACCTCACTGA ACATGTCCTG GGGCTCAGGT CAGCCAAGGA GCAACTGGAG 
AGCAATCTAT TTGAGGCCCA ACAGCAAAAT TCTGTGATAC AGGTCACCAA GGGGCAGCTG 
GAGGTCCAGA TTCAAACTAT TATCCAAGCC AAGGAAGTAA TTCAAGGGGA AGTGAAATGC 
CTGAAGCTGG AACTGGATGC TGAGAGGACC CGGGCAGAGC AGGAGTGGGA TGCAGTAGCC 
AGGCAGCTAG CCCAAGCTGA GCAGGAGGGG CAAGCATCTC TGGAGCGACA GAAGGTGGCC 
CACGAGGAGG AGGTGAACAG GCTGCAAGAG AAATGGGAGA AAGAGCGCTC CTGGCTGCAG 
CAGGAGCTGG ATAAGACCCT GGAAACCCTC GAGAGGGAGA GAGCAGAGCT GGAGACAAAG 
CTGAGAGAGC AGCAGACAGA AATGGAAGCC ATCCGTGCAC AGAGGGAAGA GGAACGAAGC 
CAGGCTGACA GCGCCCTGTA TCAAATGCAG CTAGAAACAG AGAAGGAGAG AGTATCCCTC 
CTGGAGACAC TGCTGAGGAC CCAGAAGGAG CTGGCAGATG CTAGTCAACA GCTGGAAAGG 
CTGAGACAGG ACATGAAGAT TCAGAAGTTA AAGGAACAGG AGACCACTGG CATGCTGCAG 
GCCCAGCTTC AGGAAACACA GCAGGAGCTG AAGGAGGCAG CCCAGCAGCA CAGGGACGAC 
CTTGCTGCCT TCCAGAAGGA CAAGCTGGAC CTGCAGAAGC AGGTAGAGGA CCTAATGTCT 
CAGCTGGTAG CCCACGATGA CTCCCAGAGG CTGGTGAAAG AAGAGATTGA AGAGAAGGTG 
AAAGTGGCTC AAGAATGCAG CCGGATTCAG AAGGAACTGG AGAAAGAGAA CGCCAGCCTG 
GCTCTGTCGC TGGTGGAAAA GGAAAAACGA CTTCTCATTT TACAAGAAGC TGACTCTGTA 
AGACAGCAGG AGCTGAGCTC CTTGCGCCAG GACATACAGG AGGCCCAGGA AGGGCAAAGA 
GAGCTGGGTG TACAGGTGGA ACTATTGAGG CAGGAGGTGA AGGAAAAGGA GGCTGACTTT 
GTGGCCCGGG AAGCACAGCT GCTGGAGGAG TTGGAGGCCT CACGGGTTGC AGAGCAGCAA 
CTTCGAGCGT CTTTGTGGGC CCAGGAAGCC AAGGCAACCC AGCTGCAGCT GCAGCTGCGC 
AGCACTGAGA GCCAGCTAGA GGCATTGGTT GCAGAACAAC AGCCGGAGAA CCAGGCACAG 
GCACAGCTAG CTAGCCTCTG CTCTGTTCTG CAGCAGGCCC TGGGGTCTGC ATGTGAGAGT 
AGGCCTGAGC TGAGGGGTGG AGGCGACTCT GCTCCTACCT TATGGGGCCC TGATCCAGAT 
CAGAATGGAG CTTCCAGACT CTTTAAGAGA TGGTCGCTCC CAACGGCACT GTCACCGGAG 
GCTGTGGCAC TAGCTCTCCA GAAGCTCCAT CAGGACGTGT GGAAGGCTCG GCAGGCCCGG 
GATGACCTAA GGGACCAGGT CCAGAAGCTG GTGCAGCGCC TGACTGACAC GGAGGCCCAG 
AAGAGCCAGG TTCACTCGGA GCTGCAGGAC CTGCAGAGAC AGCTCTCTCA GAGCCAGGAA 
GAGAAATCCA AGTGGGAAGG GAGGCAGAAC TCCCTGGAGT CTGAGCTGAG GGACTTGCAC 
GAGACCGCGG CATCCTTACA GAGTCGCCTC CGGCAGGCAG AACTGCAAAA AATGGAAGCT 
CAGAATGATC GAGAGTTGCT TCAGGCATCC AAGGAGAAGC TGTCAGCCCA GGTTGAACAT 
CTGCAAGCAT GTGTTGCAGA AGCCCAGGCT CAGGCAGATG CAGCTGCTGT CCTGGAAGAA 
GATCTGCGAA CTGCTCGCTC TGCACTGAAA CTGAAAAACG AGGAGCTAGA GAGTGAACGG 
GAGAGAGCCC AGGCTTTACA AGAACAGGGT GAGCTGAAGG TGGCTCAGGG GAAAGCTTTG 
CAGGAGAACC TAGCCCTGCT GGCACAGACC CTGTCTAACA GAGAGCGGGA GGTCGAGACG 
TTGCAGGCTG AGGTTCAGGA ACTGGAGAAG CAGCGGGAGA TGCAGAAGGC TGCTCTGGAG 
CTGCTCTCCC TCGACCTGAA GAAGCGCAGT CGGGAGGTAG ACCTGCAACA AGAACAGATT 
CAGGAGCTGG AGCAGTGCAG GTCAGTCTTA GAGCACTTGC CCATGGCCGT CCAAGAGCGA 
GAGCAGAAAC TGAGTGTGCA GCGGGACCAG ATCAGGGAAC TGGAGAACGA TCGGGAGGCC 
CAGAGGAGTG TCCTGGAACA CCAGCTTCTG GACCTGGAGC AGAAGGCTCA AGTGATTGAG 
TCCCAGAGGG GACAGATTCA GGATCTGAAA AAGCAACTGG GGACACTGGA ATGCCTGGCC 
CTGGAACTGG AGGAAAGCCA TCACAAAGTG GAGAGCCAAC AGAAGATGAT CACAGAACTA 
GAGGGCCAGA GGGAAATGCA GAGGGTGGCT CTAACCCACC TCACACTGGA CCTGGAAGAA 
AGGAGCCAGG AGCTGCAGGC ACAAAGCAGC CAGCTCCATG AGCTGGAGAA CCATAGCACC 
CATCTGGCAA AAGAGCTGCA GGAGAGGGAC CAGGAGGTGA CGTCCCAACG CCAGCAGATC 
GATGAGCTGC AGAAGCAGCA GGAACAGCTG GCTCAGGCCC TGGAGAGGAA AGGCCAGGAG 
CTGGTGCTGC AGAAGGAAAG GATTCAGGTT CTGGAAGATC AGAGGACGCT GCAGACCAAG 
ATCTTAGAGG AGGACCTAGA ACAGATCAAG CATTCCTTGA GAGAACGCAG CCAGGAGCTG 
GCCTCTCAGT GGCAGCTGGT GCATGAGCGT GCCGACGATG GAAAGAGCCC GAGTAAAGGC 
CAGCGAGGGA GCCTGGAGCA CTTGAAGCTG ATCCTGCGTG ATAAGGAGAA GGAGGTAGAG 
TGCCAGCAGG AGCGCATCCA GGAACTTCAG GGCCACATGG GCCAGCTGGA ACAGCAGCTG 
CAGGGTCTGC ACAGGAAGGT GGGGGAGACT AGCCTGCTCC TGACCCATCG AGAGCAGGAG 
ACAGCAACCC TGCAGCAGCA TCTCCAGGAA GCCAAGGAAC AAGGGGAACT GAGAGAGCAG 
GTACTTCAGG GTCAGCTGGA GGAGGCCCAG AGAGATCTGG CCCAGAGGGA CCATGAACTA 
GAGACCCTCC GGCAGGAAAA ACAGCAGACC CAGGACCAGG AGGAAAGCAT GAAGCTAAAG 
ACAAGTGCTC TGCAGGCCGC TCTGGAGCAG GCCCATGCGA CACTGAAGGA GCGTCAGGGG 
GAGCTGGAAG AACACAGGGA ACAGGTGCGA AGGCTCCAGG AAGAACTAGA AGTGGAGGGA 
CGGCAGGTAC GGGCCCTGGA GGAGGTGTTG GGGGACCTAA GGGCTGAGTC TCGGGAGCAC 
GAGAAGGCTG TGCTGGCCCT TCAACAACGG TGTGCTGAGC AGGCCCAGGA ACATGAGGCA 
GAAGCCAGGA CCCTGCAGGA CAGCTGGCTG CAGGCCCAGG CCACTCTCAC AGAACAAGAA 
CAAGAGCTAG CGGCACTGCG AGCGGAAAAC CAGTATTCCC GCCGACAGGA AGAGGCTGCA 
GTTAGCCAGG CAGAGGCGCT GCAGGAGGCC CTCAGCAAAG CCCAGGCTGC CCTGCAGGAG 
AAAGAGCAGA GTCTGCTTGA ACAGGCTGAG TTGAGCCACA CCCTGGAGGC CAGCACTGCC 
GCCTTGCAAG CTACCCTGGA CACCTGCCAG GCAAGTGCCC GGCAGCTGGA GGAGGCCCTG 
AGGATTCGGG AAGGTGAGAT CCAGGCCCAG GCTCTCCAAC ACCACGAGGT CACGCAGCAC 
CTCCAGCAGG AACTTTGCCA GAAGAAAGAA GAGCTGAGGC AGCTACTAGA GAAGGCTGGG 
GCCCGGAGGA GTCAAGAAAA TGGAATCCAA GAGAAGCAAA GTCTGGAGCA AGAGAGACAA 
GAGGAAACAA GGAGGCTCCT TGAGAGCCTA AAGGAGCTAC AGCTGACTGT AGCCCAAAGG 
GAAGAAGAGA TTCTGATGCT GAGGGAGGCC TCGTCCCCAC GCCACAGAGC CCTCCCAGCC 
GAGAAGCCAG CTCTGCAACC GCTCCCAGCA CAACAGGAGT TAGAGCGGCT GCAGACTGCC 
CTAAGGCAAA CAGAGGCCAG GGAGATTGAA TGGAGGGAGA AGGCTCAGGA CCTGGCTCTG 
TCCCTGGCCC AAAGCAAGGC CAGCATCAGC AGTCTGCAGG AGATAACCAT GTTCCTACAA 
GCCTCTGTTC TAGAGCGGGA ATCAGAACAA CAAAGACTAC AGGAGGAGTT GGTACTCAGC 
AGACAAGCTC TGGAAGAGCA GCAGTCAGGT GGCCCACACT CAACCAGCAG AGCAGACCAG 
GGACCAAAAG TGGGACAGGG AAGCCAGTCT GGAGAGGTAG AGACTGAGCC TAGTCCTGGA 
GTGGAGGAGA AGGAACGGTT GACACAGAGG CTTGAACGTC TGCAGCAAGC GGTGGCAGAG 
CTGGAAGTTG ACCGAAGCAA GCTGCAGTGC CACAATGCGC AGCTGCGGAC CGCCTTGGAG 
CAGGTGGAAC GAGAACGGAG GAAGCTGAAG AGGGATTCCG TGCGTGCATC CCGGGCAGGA 
TCACTGGAGG CCAGAGAAAC TATGACTTCA TCCCCCACAC AGCAGGATGG GAGAGGCAGT 
CAGAGGGGTT CCTCGGACAG CGTGCTTGTG GTTGAGCTTC AGAGAGAGGT AGCCCTGCTG 
CGAGCCCAGC TGGCCTTGGA GCGAAAGCAG AGGCAGGACT ACATTGCACG GTCCGTGCAG 
ACCAGCCGTG AGCTAGCAGG CCTGCATCAC AGCCTGTCTC ACTCCCTCCT CACCGTGGCC 
CAGGCCCCTG AGGCCACTGT TCTGGAGGCT GAGACCCGAA AGCTGGATGA GTCTCTGAAT 
CAGAGTCTGA CATCCCCAGG GCCATGTTTG CTACATCCCA GTCTGGACAC TACTCAAAAT 
ACCCACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( NM_177217.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796191.2 Mus musculus centrosomal protein 250 (Cep250), transcript variant 2, mRNA.

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CloneID	OMu01820
Clone ID Related Accession (Same CDS sequence)	NM_177217.3 , NM_008383.3
Accession Version	NM_177217.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL833786.8. On Jun 6, 2008 this sequence version replaced NM_177217.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCAGCA GGGAGCGGGA GCTGCTTCAG AAGGCCAGAG GAGAGCTGCA GCAGCAACTG 
GAAGTGCTGG AGCAGGAAGC GTGGCGACTG CGGAGGATGA ACATGGAGCT GCAGCTGCAG 
GGGGACTCAG CTCAAGGGGA GAAGCTGGAG CAGCAGGAGG AGTTGCACTT GGCTGTCCGG 
GAGAGGGAGC GCCTTCAGGA GACGCTGGTG GGCCTGGAAG CCAAGCAGTC AGAATCACTA 
AGTGAGCTGC TCACTCTTCG AGAAGCCCTG GAGTCAAGTC GCCTAGAAGG GGAGTTACTG 
AAGCAGGAGC GAGTGGAAGT GGCTGCAGCC TTGGCCAGGG CAGAACAGTC CATTGTGGAG 
CTGTCAGGTT CTGAGAACAG CCTGAAGGCA GAGGTAGCTG ATCTTCGAGC TGCAGCTGTC 
AAGCTTGGGG CCTTAAACGA GGCTTTGGCT TTAGATAAAG TTGAACTGAA CCAGCAGCTT 
CTCCAGTTGG AGCAGGAGAA CCAGTCTCTA TGCAGCAGAG TGGAGGCCGC AGAGCAGCTG 
AGAAGCGCTT TGCAGGTGGA CCTGGCGGAG GCAGAAAGGC GCAGGGAAGC CCTGTGGGAG 
AAGAAAACTC AGCTGGAGAC TCAGCTGCAG AAGGCAGAGG AAGCGGGGGC TGAGCTACAG 
GCAGAGCTGA GGGGCACCCG AGAAGAAAAG GAAGAGCTTA AAGATAAATT AAGTGAGGCA 
CATCACCAGC AAGAAACAGC TACAGCTCAC CTAGAACAGC TGCATCAGGA CGCAGAAAGA 
CAGGAAGAAA CACTTGCTAG GGCAGTCCAG GAGAAGGAAG CCCTAGTACG GGAGAGGGCA 
GCTCTTGAGG TGCGCCTGCA GGCTGTGGAG CGGGACCGAC AGGACCTCAC TGAACATGTC 
CTGGGGCTCA GGTCAGCCAA GGAGCAACTG GAGAGCAATC TATTTGAGGC CCAACAGCAA 
AATTCTGTGA TACAGGTCAC CAAGGGGCAG CTGGAGGTCC AGATTCAAAC TATTATCCAA 
GCCAAGGAAG TAATTCAAGG GGAAGTGAAA TGCCTGAAGC TGGAACTGGA TGCTGAGAGG 
ACCCGGGCAG AGCAGGAGTG GGATGCAGTA GCCAGGCAGC TAGCCCAAGC TGAGCAGGAG 
GGGCAAGCAT CTCTGGAGCG ACAGAAGGTG GCCCACGAGG AGGAGGTGAA CAGGCTGCAA 
GAGAAATGGG AGAAAGAGCG CTCCTGGCTG CAGCAGGAGC TGGATAAGAC CCTGGAAACC 
CTCGAGAGGG AGAGAGCAGA GCTGGAGACA AAGCTGAGAG AGCAGCAGAC AGAAATGGAA 
GCCATCCGTG CACAGAGGGA AGAGGAACGA AGCCAGGCTG ACAGCGCCCT GTATCAAATG 
CAGCTAGAAA CAGAGAAGGA GAGAGTATCC CTCCTGGAGA CACTGCTGAG GACCCAGAAG 
GAGCTGGCAG ATGCTAGTCA ACAGCTGGAA AGGCTGAGAC AGGACATGAA GATTCAGAAG 
TTAAAGGAAC AGGAGACCAC TGGCATGCTG CAGGCCCAGC TTCAGGAAAC ACAGCAGGAG 
CTGAAGGAGG CAGCCCAGCA GCACAGGGAC GACCTTGCTG CCTTCCAGAA GGACAAGCTG 
GACCTGCAGA AGCAGGTAGA GGACCTAATG TCTCAGCTGG TAGCCCACGA TGACTCCCAG 
AGGCTGGTGA AAGAAGAGAT TGAAGAGAAG GTGAAAGTGG CTCAAGAATG CAGCCGGATT 
CAGAAGGAAC TGGAGAAAGA GAACGCCAGC CTGGCTCTGT CGCTGGTGGA AAAGGAAAAA 
CGACTTCTCA TTTTACAAGA AGCTGACTCT GTAAGACAGC AGGAGCTGAG CTCCTTGCGC 
CAGGACATAC AGGAGGCCCA GGAAGGGCAA AGAGAGCTGG GTGTACAGGT GGAACTATTG 
AGGCAGGAGG TGAAGGAAAA GGAGGCTGAC TTTGTGGCCC GGGAAGCACA GCTGCTGGAG 
GAGTTGGAGG CCTCACGGGT TGCAGAGCAG CAACTTCGAG CGTCTTTGTG GGCCCAGGAA 
GCCAAGGCAA CCCAGCTGCA GCTGCAGCTG CGCAGCACTG AGAGCCAGCT AGAGGCATTG 
GTTGCAGAAC AACAGCCGGA GAACCAGGCA CAGGCACAGC TAGCTAGCCT CTGCTCTGTT 
CTGCAGCAGG CCCTGGGGTC TGCATGTGAG AGTAGGCCTG AGCTGAGGGG TGGAGGCGAC 
TCTGCTCCTA CCTTATGGGG CCCTGATCCA GATCAGAATG GAGCTTCCAG ACTCTTTAAG 
AGATGGTCGC TCCCAACGGC ACTGTCACCG GAGGCTGTGG CACTAGCTCT CCAGAAGCTC 
CATCAGGACG TGTGGAAGGC TCGGCAGGCC CGGGATGACC TAAGGGACCA GGTCCAGAAG 
CTGGTGCAGC GCCTGACTGA CACGGAGGCC CAGAAGAGCC AGGTTCACTC GGAGCTGCAG 
GACCTGCAGA GACAGCTCTC TCAGAGCCAG GAAGAGAAAT CCAAGTGGGA AGGGAGGCAG 
AACTCCCTGG AGTCTGAGCT GAGGGACTTG CACGAGACCG CGGCATCCTT ACAGAGTCGC 
CTCCGGCAGG CAGAACTGCA AAAAATGGAA GCTCAGAATG ATCGAGAGTT GCTTCAGGCA 
TCCAAGGAGA AGCTGTCAGC CCAGGTTGAA CATCTGCAAG CATGTGTTGC AGAAGCCCAG 
GCTCAGGCAG ATGCAGCTGC TGTCCTGGAA GAAGATCTGC GAACTGCTCG CTCTGCACTG 
AAACTGAAAA ACGAGGAGCT AGAGAGTGAA CGGGAGAGAG CCCAGGCTTT ACAAGAACAG 
GGTGAGCTGA AGGTGGCTCA GGGGAAAGCT TTGCAGGAGA ACCTAGCCCT GCTGGCACAG 
ACCCTGTCTA ACAGAGAGCG GGAGGTCGAG ACGTTGCAGG CTGAGGTTCA GGAACTGGAG 
AAGCAGCGGG AGATGCAGAA GGCTGCTCTG GAGCTGCTCT CCCTCGACCT GAAGAAGCGC 
AGTCGGGAGG TAGACCTGCA ACAAGAACAG ATTCAGGAGC TGGAGCAGTG CAGGTCAGTC 
TTAGAGCACT TGCCCATGGC CGTCCAAGAG CGAGAGCAGA AACTGAGTGT GCAGCGGGAC 
CAGATCAGGG AACTGGAGAA CGATCGGGAG GCCCAGAGGA GTGTCCTGGA ACACCAGCTT 
CTGGACCTGG AGCAGAAGGC TCAAGTGATT GAGTCCCAGA GGGGACAGAT TCAGGATCTG 
AAAAAGCAAC TGGGGACACT GGAATGCCTG GCCCTGGAAC TGGAGGAAAG CCATCACAAA 
GTGGAGAGCC AACAGAAGAT GATCACAGAA CTAGAGGGCC AGAGGGAAAT GCAGAGGGTG 
GCTCTAACCC ACCTCACACT GGACCTGGAA GAAAGGAGCC AGGAGCTGCA GGCACAAAGC 
AGCCAGCTCC ATGAGCTGGA GAACCATAGC ACCCATCTGG CAAAAGAGCT GCAGGAGAGG 
GACCAGGAGG TGACGTCCCA ACGCCAGCAG ATCGATGAGC TGCAGAAGCA GCAGGAACAG 
CTGGCTCAGG CCCTGGAGAG GAAAGGCCAG GAGCTGGTGC TGCAGAAGGA AAGGATTCAG 
GTTCTGGAAG ATCAGAGGAC GCTGCAGACC AAGATCTTAG AGGAGGACCT AGAACAGATC 
AAGCATTCCT TGAGAGAACG CAGCCAGGAG CTGGCCTCTC AGTGGCAGCT GGTGCATGAG 
CGTGCCGACG ATGGAAAGAG CCCGAGTAAA GGCCAGCGAG GGAGCCTGGA GCACTTGAAG 
CTGATCCTGC GTGATAAGGA GAAGGAGGTA GAGTGCCAGC AGGAGCGCAT CCAGGAACTT 
CAGGGCCACA TGGGCCAGCT GGAACAGCAG CTGCAGGGTC TGCACAGGAA GGTGGGGGAG 
ACTAGCCTGC TCCTGACCCA TCGAGAGCAG GAGACAGCAA CCCTGCAGCA GCATCTCCAG 
GAAGCCAAGG AACAAGGGGA ACTGAGAGAG CAGGTACTTC AGGGTCAGCT GGAGGAGGCC 
CAGAGAGATC TGGCCCAGAG GGACCATGAA CTAGAGACCC TCCGGCAGGA AAAACAGCAG 
ACCCAGGACC AGGAGGAAAG CATGAAGCTA AAGACAAGTG CTCTGCAGGC CGCTCTGGAG 
CAGGCCCATG CGACACTGAA GGAGCGTCAG GGGGAGCTGG AAGAACACAG GGAACAGGTG 
CGAAGGCTCC AGGAAGAACT AGAAGTGGAG GGACGGCAGG TACGGGCCCT GGAGGAGGTG 
TTGGGGGACC TAAGGGCTGA GTCTCGGGAG CACGAGAAGG CTGTGCTGGC CCTTCAACAA 
CGGTGTGCTG AGCAGGCCCA GGAACATGAG GCAGAAGCCA GGACCCTGCA GGACAGCTGG 
CTGCAGGCCC AGGCCACTCT CACAGAACAA GAACAAGAGC TAGCGGCACT GCGAGCGGAA 
AACCAGTATT CCCGCCGACA GGAAGAGGCT GCAGTTAGCC AGGCAGAGGC GCTGCAGGAG 
GCCCTCAGCA AAGCCCAGGC TGCCCTGCAG GAGAAAGAGC AGAGTCTGCT TGAACAGGCT 
GAGTTGAGCC ACACCCTGGA GGCCAGCACT GCCGCCTTGC AAGCTACCCT GGACACCTGC 
CAGGCAAGTG CCCGGCAGCT GGAGGAGGCC CTGAGGATTC GGGAAGGTGA GATCCAGGCC 
CAGGCTCTCC AACACCACGA GGTCACGCAG CACCTCCAGC AGGAACTTTG CCAGAAGAAA 
GAAGAGCTGA GGCAGCTACT AGAGAAGGCT GGGGCCCGGA GGAGTCAAGA AAATGGAATC 
CAAGAGAAGC AAAGTCTGGA GCAAGAGAGA CAAGAGGAAA CAAGGAGGCT CCTTGAGAGC 
CTAAAGGAGC TACAGCTGAC TGTAGCCCAA AGGGAAGAAG AGATTCTGAT GCTGAGGGAG 
GCCTCGTCCC CACGCCACAG AGCCCTCCCA GCCGAGAAGC CAGCTCTGCA ACCGCTCCCA 
GCACAACAGG AGTTAGAGCG GCTGCAGACT GCCCTAAGGC AAACAGAGGC CAGGGAGATT 
GAATGGAGGG AGAAGGCTCA GGACCTGGCT CTGTCCCTGG CCCAAAGCAA GGCCAGCATC 
AGCAGTCTGC AGGAGATAAC CATGTTCCTA CAAGCCTCTG TTCTAGAGCG GGAATCAGAA 
CAACAAAGAC TACAGGAGGA GTTGGTACTC AGCAGACAAG CTCTGGAAGA GCAGCAGTCA 
GGTGGCCCAC ACTCAACCAG CAGAGCAGAC CAGGGACCAA AAGTGGGACA GGGAAGCCAG 
TCTGGAGAGG TAGAGACTGA GCCTAGTCCT GGAGTGGAGG AGAAGGAACG GTTGACACAG 
AGGCTTGAAC GTCTGCAGCA AGCGGTGGCA GAGCTGGAAG TTGACCGAAG CAAGCTGCAG 
TGCCACAATG CGCAGCTGCG GACCGCCTTG GAGCAGGTGG AACGAGAACG GAGGAAGCTG 
AAGAGGGATT CCGTGCGTGC ATCCCGGGCA GGATCACTGG AGGCCAGAGA AACTATGACT 
TCATCCCCCA CACAGCAGGA TGGGAGAGGC AGTCAGAGGG GTTCCTCGGA CAGCGTGCTT 
GTGGTTGAGC TTCAGAGAGA GGTAGCCCTG CTGCGAGCCC AGCTGGCCTT GGAGCGAAAG 
CAGAGGCAGG ACTACATTGC ACGGTCCGTG CAGACCAGCC GTGAGCTAGC AGGCCTGCAT 
CACAGCCTGT CTCACTCCCT CCTCACCGTG GCCCAGGCCC CTGAGGCCAC TGTTCTGGAG 
GCTGAGACCC GAAAGCTGGA TGAGTCTCTG AATCAGAGTC TGACATCCCC AGGGCCATGT 
TTGCTACATC CCAGTCTGGA CACTACTCAA AATACCCACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796191.2
CDS	201..7445
Translation	METGSPGLNMKPQSLQLVLEGQVLALQQQMAENQAASWRKLKNSQEAQKRQATLVRKLQAKVLQYRSWCQDLEKRLEATGGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKLTVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	NM_177217.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177217.3

ORF Insert Sequence:

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCAGCA GGGAGCGGGA GCTGCTTCAG AAGGCCAGAG GAGAGCTGCA GCAGCAACTG 
GAAGTGCTGG AGCAGGAAGC GTGGCGACTG CGGAGGATGA ACATGGAGCT GCAGCTGCAG 
GGGGACTCAG CTCAAGGGGA GAAGCTGGAG CAGCAGGAGG AGTTGCACTT GGCTGTCCGG 
GAGAGGGAGC GCCTTCAGGA GACGCTGGTG GGCCTGGAAG CCAAGCAGTC AGAATCACTA 
AGTGAGCTGC TCACTCTTCG AGAAGCCCTG GAGTCAAGTC GCCTAGAAGG GGAGTTACTG 
AAGCAGGAGC GAGTGGAAGT GGCTGCAGCC TTGGCCAGGG CAGAACAGTC CATTGTGGAG 
CTGTCAGGTT CTGAGAACAG CCTGAAGGCA GAGGTAGCTG ATCTTCGAGC TGCAGCTGTC 
AAGCTTGGGG CCTTAAACGA GGCTTTGGCT TTAGATAAAG TTGAACTGAA CCAGCAGCTT 
CTCCAGTTGG AGCAGGAGAA CCAGTCTCTA TGCAGCAGAG TGGAGGCCGC AGAGCAGCTG 
AGAAGCGCTT TGCAGGTGGA CCTGGCGGAG GCAGAAAGGC GCAGGGAAGC CCTGTGGGAG 
AAGAAAACTC AGCTGGAGAC TCAGCTGCAG AAGGCAGAGG AAGCGGGGGC TGAGCTACAG 
GCAGAGCTGA GGGGCACCCG AGAAGAAAAG GAAGAGCTTA AAGATAAATT AAGTGAGGCA 
CATCACCAGC AAGAAACAGC TACAGCTCAC CTAGAACAGC TGCATCAGGA CGCAGAAAGA 
CAGGAAGAAA CACTTGCTAG GGCAGTCCAG GAGAAGGAAG CCCTAGTACG GGAGAGGGCA 
GCTCTTGAGG TGCGCCTGCA GGCTGTGGAG CGGGACCGAC AGGACCTCAC TGAACATGTC 
CTGGGGCTCA GGTCAGCCAA GGAGCAACTG GAGAGCAATC TATTTGAGGC CCAACAGCAA 
AATTCTGTGA TACAGGTCAC CAAGGGGCAG CTGGAGGTCC AGATTCAAAC TATTATCCAA 
GCCAAGGAAG TAATTCAAGG GGAAGTGAAA TGCCTGAAGC TGGAACTGGA TGCTGAGAGG 
ACCCGGGCAG AGCAGGAGTG GGATGCAGTA GCCAGGCAGC TAGCCCAAGC TGAGCAGGAG 
GGGCAAGCAT CTCTGGAGCG ACAGAAGGTG GCCCACGAGG AGGAGGTGAA CAGGCTGCAA 
GAGAAATGGG AGAAAGAGCG CTCCTGGCTG CAGCAGGAGC TGGATAAGAC CCTGGAAACC 
CTCGAGAGGG AGAGAGCAGA GCTGGAGACA AAGCTGAGAG AGCAGCAGAC AGAAATGGAA 
GCCATCCGTG CACAGAGGGA AGAGGAACGA AGCCAGGCTG ACAGCGCCCT GTATCAAATG 
CAGCTAGAAA CAGAGAAGGA GAGAGTATCC CTCCTGGAGA CACTGCTGAG GACCCAGAAG 
GAGCTGGCAG ATGCTAGTCA ACAGCTGGAA AGGCTGAGAC AGGACATGAA GATTCAGAAG 
TTAAAGGAAC AGGAGACCAC TGGCATGCTG CAGGCCCAGC TTCAGGAAAC ACAGCAGGAG 
CTGAAGGAGG CAGCCCAGCA GCACAGGGAC GACCTTGCTG CCTTCCAGAA GGACAAGCTG 
GACCTGCAGA AGCAGGTAGA GGACCTAATG TCTCAGCTGG TAGCCCACGA TGACTCCCAG 
AGGCTGGTGA AAGAAGAGAT TGAAGAGAAG GTGAAAGTGG CTCAAGAATG CAGCCGGATT 
CAGAAGGAAC TGGAGAAAGA GAACGCCAGC CTGGCTCTGT CGCTGGTGGA AAAGGAAAAA 
CGACTTCTCA TTTTACAAGA AGCTGACTCT GTAAGACAGC AGGAGCTGAG CTCCTTGCGC 
CAGGACATAC AGGAGGCCCA GGAAGGGCAA AGAGAGCTGG GTGTACAGGT GGAACTATTG 
AGGCAGGAGG TGAAGGAAAA GGAGGCTGAC TTTGTGGCCC GGGAAGCACA GCTGCTGGAG 
GAGTTGGAGG CCTCACGGGT TGCAGAGCAG CAACTTCGAG CGTCTTTGTG GGCCCAGGAA 
GCCAAGGCAA CCCAGCTGCA GCTGCAGCTG CGCAGCACTG AGAGCCAGCT AGAGGCATTG 
GTTGCAGAAC AACAGCCGGA GAACCAGGCA CAGGCACAGC TAGCTAGCCT CTGCTCTGTT 
CTGCAGCAGG CCCTGGGGTC TGCATGTGAG AGTAGGCCTG AGCTGAGGGG TGGAGGCGAC 
TCTGCTCCTA CCTTATGGGG CCCTGATCCA GATCAGAATG GAGCTTCCAG ACTCTTTAAG 
AGATGGTCGC TCCCAACGGC ACTGTCACCG GAGGCTGTGG CACTAGCTCT CCAGAAGCTC 
CATCAGGACG TGTGGAAGGC TCGGCAGGCC CGGGATGACC TAAGGGACCA GGTCCAGAAG 
CTGGTGCAGC GCCTGACTGA CACGGAGGCC CAGAAGAGCC AGGTTCACTC GGAGCTGCAG 
GACCTGCAGA GACAGCTCTC TCAGAGCCAG GAAGAGAAAT CCAAGTGGGA AGGGAGGCAG 
AACTCCCTGG AGTCTGAGCT GAGGGACTTG CACGAGACCG CGGCATCCTT ACAGAGTCGC 
CTCCGGCAGG CAGAACTGCA AAAAATGGAA GCTCAGAATG ATCGAGAGTT GCTTCAGGCA 
TCCAAGGAGA AGCTGTCAGC CCAGGTTGAA CATCTGCAAG CATGTGTTGC AGAAGCCCAG 
GCTCAGGCAG ATGCAGCTGC TGTCCTGGAA GAAGATCTGC GAACTGCTCG CTCTGCACTG 
AAACTGAAAA ACGAGGAGCT AGAGAGTGAA CGGGAGAGAG CCCAGGCTTT ACAAGAACAG 
GGTGAGCTGA AGGTGGCTCA GGGGAAAGCT TTGCAGGAGA ACCTAGCCCT GCTGGCACAG 
ACCCTGTCTA ACAGAGAGCG GGAGGTCGAG ACGTTGCAGG CTGAGGTTCA GGAACTGGAG 
AAGCAGCGGG AGATGCAGAA GGCTGCTCTG GAGCTGCTCT CCCTCGACCT GAAGAAGCGC 
AGTCGGGAGG TAGACCTGCA ACAAGAACAG ATTCAGGAGC TGGAGCAGTG CAGGTCAGTC 
TTAGAGCACT TGCCCATGGC CGTCCAAGAG CGAGAGCAGA AACTGAGTGT GCAGCGGGAC 
CAGATCAGGG AACTGGAGAA CGATCGGGAG GCCCAGAGGA GTGTCCTGGA ACACCAGCTT 
CTGGACCTGG AGCAGAAGGC TCAAGTGATT GAGTCCCAGA GGGGACAGAT TCAGGATCTG 
AAAAAGCAAC TGGGGACACT GGAATGCCTG GCCCTGGAAC TGGAGGAAAG CCATCACAAA 
GTGGAGAGCC AACAGAAGAT GATCACAGAA CTAGAGGGCC AGAGGGAAAT GCAGAGGGTG 
GCTCTAACCC ACCTCACACT GGACCTGGAA GAAAGGAGCC AGGAGCTGCA GGCACAAAGC 
AGCCAGCTCC ATGAGCTGGA GAACCATAGC ACCCATCTGG CAAAAGAGCT GCAGGAGAGG 
GACCAGGAGG TGACGTCCCA ACGCCAGCAG ATCGATGAGC TGCAGAAGCA GCAGGAACAG 
CTGGCTCAGG CCCTGGAGAG GAAAGGCCAG GAGCTGGTGC TGCAGAAGGA AAGGATTCAG 
GTTCTGGAAG ATCAGAGGAC GCTGCAGACC AAGATCTTAG AGGAGGACCT AGAACAGATC 
AAGCATTCCT TGAGAGAACG CAGCCAGGAG CTGGCCTCTC AGTGGCAGCT GGTGCATGAG 
CGTGCCGACG ATGGAAAGAG CCCGAGTAAA GGCCAGCGAG GGAGCCTGGA GCACTTGAAG 
CTGATCCTGC GTGATAAGGA GAAGGAGGTA GAGTGCCAGC AGGAGCGCAT CCAGGAACTT 
CAGGGCCACA TGGGCCAGCT GGAACAGCAG CTGCAGGGTC TGCACAGGAA GGTGGGGGAG 
ACTAGCCTGC TCCTGACCCA TCGAGAGCAG GAGACAGCAA CCCTGCAGCA GCATCTCCAG 
GAAGCCAAGG AACAAGGGGA ACTGAGAGAG CAGGTACTTC AGGGTCAGCT GGAGGAGGCC 
CAGAGAGATC TGGCCCAGAG GGACCATGAA CTAGAGACCC TCCGGCAGGA AAAACAGCAG 
ACCCAGGACC AGGAGGAAAG CATGAAGCTA AAGACAAGTG CTCTGCAGGC CGCTCTGGAG 
CAGGCCCATG CGACACTGAA GGAGCGTCAG GGGGAGCTGG AAGAACACAG GGAACAGGTG 
CGAAGGCTCC AGGAAGAACT AGAAGTGGAG GGACGGCAGG TACGGGCCCT GGAGGAGGTG 
TTGGGGGACC TAAGGGCTGA GTCTCGGGAG CACGAGAAGG CTGTGCTGGC CCTTCAACAA 
CGGTGTGCTG AGCAGGCCCA GGAACATGAG GCAGAAGCCA GGACCCTGCA GGACAGCTGG 
CTGCAGGCCC AGGCCACTCT CACAGAACAA GAACAAGAGC TAGCGGCACT GCGAGCGGAA 
AACCAGTATT CCCGCCGACA GGAAGAGGCT GCAGTTAGCC AGGCAGAGGC GCTGCAGGAG 
GCCCTCAGCA AAGCCCAGGC TGCCCTGCAG GAGAAAGAGC AGAGTCTGCT TGAACAGGCT 
GAGTTGAGCC ACACCCTGGA GGCCAGCACT GCCGCCTTGC AAGCTACCCT GGACACCTGC 
CAGGCAAGTG CCCGGCAGCT GGAGGAGGCC CTGAGGATTC GGGAAGGTGA GATCCAGGCC 
CAGGCTCTCC AACACCACGA GGTCACGCAG CACCTCCAGC AGGAACTTTG CCAGAAGAAA 
GAAGAGCTGA GGCAGCTACT AGAGAAGGCT GGGGCCCGGA GGAGTCAAGA AAATGGAATC 
CAAGAGAAGC AAAGTCTGGA GCAAGAGAGA CAAGAGGAAA CAAGGAGGCT CCTTGAGAGC 
CTAAAGGAGC TACAGCTGAC TGTAGCCCAA AGGGAAGAAG AGATTCTGAT GCTGAGGGAG 
GCCTCGTCCC CACGCCACAG AGCCCTCCCA GCCGAGAAGC CAGCTCTGCA ACCGCTCCCA 
GCACAACAGG AGTTAGAGCG GCTGCAGACT GCCCTAAGGC AAACAGAGGC CAGGGAGATT 
GAATGGAGGG AGAAGGCTCA GGACCTGGCT CTGTCCCTGG CCCAAAGCAA GGCCAGCATC 
AGCAGTCTGC AGGAGATAAC CATGTTCCTA CAAGCCTCTG TTCTAGAGCG GGAATCAGAA 
CAACAAAGAC TACAGGAGGA GTTGGTACTC AGCAGACAAG CTCTGGAAGA GCAGCAGTCA 
GGTGGCCCAC ACTCAACCAG CAGAGCAGAC CAGGGACCAA AAGTGGGACA GGGAAGCCAG 
TCTGGAGAGG TAGAGACTGA GCCTAGTCCT GGAGTGGAGG AGAAGGAACG GTTGACACAG 
AGGCTTGAAC GTCTGCAGCA AGCGGTGGCA GAGCTGGAAG TTGACCGAAG CAAGCTGCAG 
TGCCACAATG CGCAGCTGCG GACCGCCTTG GAGCAGGTGG AACGAGAACG GAGGAAGCTG 
AAGAGGGATT CCGTGCGTGC ATCCCGGGCA GGATCACTGG AGGCCAGAGA AACTATGACT 
TCATCCCCCA CACAGCAGGA TGGGAGAGGC AGTCAGAGGG GTTCCTCGGA CAGCGTGCTT 
GTGGTTGAGC TTCAGAGAGA GGTAGCCCTG CTGCGAGCCC AGCTGGCCTT GGAGCGAAAG 
CAGAGGCAGG ACTACATTGC ACGGTCCGTG CAGACCAGCC GTGAGCTAGC AGGCCTGCAT 
CACAGCCTGT CTCACTCCCT CCTCACCGTG GCCCAGGCCC CTGAGGCCAC TGTTCTGGAG 
GCTGAGACCC GAAAGCTGGA TGAGTCTCTG AATCAGAGTC TGACATCCCC AGGGCCATGT 
TTGCTACATC CCAGTCTGGA CACTACTCAA AATACCCACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( NM_008383.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032409.3 Mus musculus centrosomal protein 250 (Cep250), transcript variant 1, mRNA.

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CloneID	OMu01820
Clone ID Related Accession (Same CDS sequence)	NM_177217.3 , NM_008383.3
Accession Version	NM_008383.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL833786.8. On Jun 6, 2008 this sequence version replaced NM_008383.2. Transcript Variant: This variant (1) encodes the shortest isoform (1). Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCAGCA GGGAGCGGGA GCTGCTTCAG AAGGCCAGAG GAGAGCTGCA GCAGCAACTG 
GAAGTGCTGG AGCAGGAAGC GTGGCGACTG CGGAGGATGA ACATGGAGCT GCAGCTGCAG 
GGGGACTCAG CTCAAGGGGA GAAGCTGGAG CAGCAGGAGG AGTTGCACTT GGCTGTCCGG 
GAGAGGGAGC GCCTTCAGGA GACGCTGGTG GGCCTGGAAG CCAAGCAGTC AGAATCACTA 
AGTGAGCTGC TCACTCTTCG AGAAGCCCTG GAGTCAAGTC GCCTAGAAGG GGAGTTACTG 
AAGCAGGAGC GAGTGGAAGT GGCTGCAGCC TTGGCCAGGG CAGAACAGTC CATTGTGGAG 
CTGTCAGGTT CTGAGAACAG CCTGAAGGCA GAGGTAGCTG ATCTTCGAGC TGCAGCTGTC 
AAGCTTGGGG CCTTAAACGA GGCTTTGGCT TTAGATAAAG TTGAACTGAA CCAGCAGCTT 
CTCCAGTTGG AGCAGGAGAA CCAGTCTCTA TGCAGCAGAG TGGAGGCCGC AGAGCAGCTG 
AGAAGCGCTT TGCAGGTGGA CCTGGCGGAG GCAGAAAGGC GCAGGGAAGC CCTGTGGGAG 
AAGAAAACTC AGCTGGAGAC TCAGCTGCAG AAGGCAGAGG AAGCGGGGGC TGAGCTACAG 
GCAGAGCTGA GGGGCACCCG AGAAGAAAAG GAAGAGCTTA AAGATAAATT AAGTGAGGCA 
CATCACCAGC AAGAAACAGC TACAGCTCAC CTAGAACAGC TGCATCAGGA CGCAGAAAGA 
CAGGAAGAAA CACTTGCTAG GGCAGTCCAG GAGAAGGAAG CCCTAGTACG GGAGAGGGCA 
GCTCTTGAGG TGCGCCTGCA GGCTGTGGAG CGGGACCGAC AGGACCTCAC TGAACATGTC 
CTGGGGCTCA GGTCAGCCAA GGAGCAACTG GAGAGCAATC TATTTGAGGC CCAACAGCAA 
AATTCTGTGA TACAGGTCAC CAAGGGGCAG CTGGAGGTCC AGATTCAAAC TATTATCCAA 
GCCAAGGAAG TAATTCAAGG GGAAGTGAAA TGCCTGAAGC TGGAACTGGA TGCTGAGAGG 
ACCCGGGCAG AGCAGGAGTG GGATGCAGTA GCCAGGCAGC TAGCCCAAGC TGAGCAGGAG 
GGGCAAGCAT CTCTGGAGCG ACAGAAGGTG GCCCACGAGG AGGAGGTGAA CAGGCTGCAA 
GAGAAATGGG AGAAAGAGCG CTCCTGGCTG CAGCAGGAGC TGGATAAGAC CCTGGAAACC 
CTCGAGAGGG AGAGAGCAGA GCTGGAGACA AAGCTGAGAG AGCAGCAGAC AGAAATGGAA 
GCCATCCGTG CACAGAGGGA AGAGGAACGA AGCCAGGCTG ACAGCGCCCT GTATCAAATG 
CAGCTAGAAA CAGAGAAGGA GAGAGTATCC CTCCTGGAGA CACTGCTGAG GACCCAGAAG 
GAGCTGGCAG ATGCTAGTCA ACAGCTGGAA AGGCTGAGAC AGGACATGAA GATTCAGAAG 
TTAAAGGAAC AGGAGACCAC TGGCATGCTG CAGGCCCAGC TTCAGGAAAC ACAGCAGGAG 
CTGAAGGAGG CAGCCCAGCA GCACAGGGAC GACCTTGCTG CCTTCCAGAA GGACAAGCTG 
GACCTGCAGA AGCAGGTAGA GGACCTAATG TCTCAGCTGG TAGCCCACGA TGACTCCCAG 
AGGCTGGTGA AAGAAGAGAT TGAAGAGAAG GTGAAAGTGG CTCAAGAATG CAGCCGGATT 
CAGAAGGAAC TGGAGAAAGA GAACGCCAGC CTGGCTCTGT CGCTGGTGGA AAAGGAAAAA 
CGACTTCTCA TTTTACAAGA AGCTGACTCT GTAAGACAGC AGGAGCTGAG CTCCTTGCGC 
CAGGACATAC AGGAGGCCCA GGAAGGGCAA AGAGAGCTGG GTGTACAGGT GGAACTATTG 
AGGCAGGAGG TGAAGGAAAA GGAGGCTGAC TTTGTGGCCC GGGAAGCACA GCTGCTGGAG 
GAGTTGGAGG CCTCACGGGT TGCAGAGCAG CAACTTCGAG CGTCTTTGTG GGCCCAGGAA 
GCCAAGGCAA CCCAGCTGCA GCTGCAGCTG CGCAGCACTG AGAGCCAGCT AGAGGCATTG 
GTTGCAGAAC AACAGCCGGA GAACCAGGCA CAGGCACAGC TAGCTAGCCT CTGCTCTGTT 
CTGCAGCAGG CCCTGGGGTC TGCATGTGAG AGTAGGCCTG AGCTGAGGGG TGGAGGCGAC 
TCTGCTCCTA CCTTATGGGG CCCTGATCCA GATCAGAATG GAGCTTCCAG ACTCTTTAAG 
AGATGGTCGC TCCCAACGGC ACTGTCACCG GAGGCTGTGG CACTAGCTCT CCAGAAGCTC 
CATCAGGACG TGTGGAAGGC TCGGCAGGCC CGGGATGACC TAAGGGACCA GGTCCAGAAG 
CTGGTGCAGC GCCTGACTGA CACGGAGGCC CAGAAGAGCC AGGTTCACTC GGAGCTGCAG 
GACCTGCAGA GACAGCTCTC TCAGAGCCAG GAAGAGAAAT CCAAGTGGGA AGGGAGGCAG 
AACTCCCTGG AGTCTGAGCT GAGGGACTTG CACGAGACCG CGGCATCCTT ACAGAGTCGC 
CTCCGGCAGG CAGAACTGCA AAAAATGGAA GCTCAGAATG ATCGAGAGTT GCTTCAGGCA 
TCCAAGGAGA AGCTGTCAGC CCAGGTTGAA CATCTGCAAG CATGTGTTGC AGAAGCCCAG 
GCTCAGGCAG ATGCAGCTGC TGTCCTGGAA GAAGATCTGC GAACTGCTCG CTCTGCACTG 
AAACTGAAAA ACGAGGAGCT AGAGAGTGAA CGGGAGAGAG CCCAGGCTTT ACAAGAACAG 
GGTGAGCTGA AGGTGGCTCA GGGGAAAGCT TTGCAGGAGA ACCTAGCCCT GCTGGCACAG 
ACCCTGTCTA ACAGAGAGCG GGAGGTCGAG ACGTTGCAGG CTGAGGTTCA GGAACTGGAG 
AAGCAGCGGG AGATGCAGAA GGCTGCTCTG GAGCTGCTCT CCCTCGACCT GAAGAAGCGC 
AGTCGGGAGG TAGACCTGCA ACAAGAACAG ATTCAGGAGC TGGAGCAGTG CAGGTCAGTC 
TTAGAGCACT TGCCCATGGC CGTCCAAGAG CGAGAGCAGA AACTGAGTGT GCAGCGGGAC 
CAGATCAGGG AACTGGAGAA CGATCGGGAG GCCCAGAGGA GTGTCCTGGA ACACCAGCTT 
CTGGACCTGG AGCAGAAGGC TCAAGTGATT GAGTCCCAGA GGGGACAGAT TCAGGATCTG 
AAAAAGCAAC TGGGGACACT GGAATGCCTG GCCCTGGAAC TGGAGGAAAG CCATCACAAA 
GTGGAGAGCC AACAGAAGAT GATCACAGAA CTAGAGGGCC AGAGGGAAAT GCAGAGGGTG 
GCTCTAACCC ACCTCACACT GGACCTGGAA GAAAGGAGCC AGGAGCTGCA GGCACAAAGC 
AGCCAGCTCC ATGAGCTGGA GAACCATAGC ACCCATCTGG CAAAAGAGCT GCAGGAGAGG 
GACCAGGAGG TGACGTCCCA ACGCCAGCAG ATCGATGAGC TGCAGAAGCA GCAGGAACAG 
CTGGCTCAGG CCCTGGAGAG GAAAGGCCAG GAGCTGGTGC TGCAGAAGGA AAGGATTCAG 
GTTCTGGAAG ATCAGAGGAC GCTGCAGACC AAGATCTTAG AGGAGGACCT AGAACAGATC 
AAGCATTCCT TGAGAGAACG CAGCCAGGAG CTGGCCTCTC AGTGGCAGCT GGTGCATGAG 
CGTGCCGACG ATGGAAAGAG CCCGAGTAAA GGCCAGCGAG GGAGCCTGGA GCACTTGAAG 
CTGATCCTGC GTGATAAGGA GAAGGAGGTA GAGTGCCAGC AGGAGCGCAT CCAGGAACTT 
CAGGGCCACA TGGGCCAGCT GGAACAGCAG CTGCAGGGTC TGCACAGGAA GGTGGGGGAG 
ACTAGCCTGC TCCTGACCCA TCGAGAGCAG GAGACAGCAA CCCTGCAGCA GCATCTCCAG 
GAAGCCAAGG AACAAGGGGA ACTGAGAGAG CAGGTACTTC AGGGTCAGCT GGAGGAGGCC 
CAGAGAGATC TGGCCCAGAG GGACCATGAA CTAGAGACCC TCCGGCAGGA AAAACAGCAG 
ACCCAGGACC AGGAGGAAAG CATGAAGCTA AAGACAAGTG CTCTGCAGGC CGCTCTGGAG 
CAGGCCCATG CGACACTGAA GGAGCGTCAG GGGGAGCTGG AAGAACACAG GGAACAGGTG 
CGAAGGCTCC AGGAAGAACT AGAAGTGGAG GGACGGCAGG TACGGGCCCT GGAGGAGGTG 
TTGGGGGACC TAAGGGCTGA GTCTCGGGAG CACGAGAAGG CTGTGCTGGC CCTTCAACAA 
CGGTGTGCTG AGCAGGCCCA GGAACATGAG GCAGAAGCCA GGACCCTGCA GGACAGCTGG 
CTGCAGGCCC AGGCCACTCT CACAGAACAA GAACAAGAGC TAGCGGCACT GCGAGCGGAA 
AACCAGTATT CCCGCCGACA GGAAGAGGCT GCAGTTAGCC AGGCAGAGGC GCTGCAGGAG 
GCCCTCAGCA AAGCCCAGGC TGCCCTGCAG GAGAAAGAGC AGAGTCTGCT TGAACAGGCT 
GAGTTGAGCC ACACCCTGGA GGCCAGCACT GCCGCCTTGC AAGCTACCCT GGACACCTGC 
CAGGCAAGTG CCCGGCAGCT GGAGGAGGCC CTGAGGATTC GGGAAGGTGA GATCCAGGCC 
CAGGCTCTCC AACACCACGA GGTCACGCAG CACCTCCAGC AGGAACTTTG CCAGAAGAAA 
GAAGAGCTGA GGCAGCTACT AGAGAAGGCT GGGGCCCGGA GGAGTCAAGA AAATGGAATC 
CAAGAGAAGC AAAGTCTGGA GCAAGAGAGA CAAGAGGAAA CAAGGAGGCT CCTTGAGAGC 
CTAAAGGAGC TACAGCTGAC TGTAGCCCAA AGGGAAGAAG AGATTCTGAT GCTGAGGGAG 
GCCTCGTCCC CACGCCACAG AGCCCTCCCA GCCGAGAAGC CAGCTCTGCA ACCGCTCCCA 
GCACAACAGG AGTTAGAGCG GCTGCAGACT GCCCTAAGGC AAACAGAGGC CAGGGAGATT 
GAATGGAGGG AGAAGGCTCA GGACCTGGCT CTGTCCCTGG CCCAAAGCAA GGCCAGCATC 
AGCAGTCTGC AGGAGATAAC CATGTTCCTA CAAGCCTCTG TTCTAGAGCG GGAATCAGAA 
CAACAAAGAC TACAGGAGGA GTTGGTACTC AGCAGACAAG CTCTGGAAGA GCAGCAGTCA 
GGTGGCCCAC ACTCAACCAG CAGAGCAGAC CAGGGACCAA AAGTGGGACA GGGAAGCCAG 
TCTGGAGAGG TAGAGACTGA GCCTAGTCCT GGAGTGGAGG AGAAGGAACG GTTGACACAG 
AGGCTTGAAC GTCTGCAGCA AGCGGTGGCA GAGCTGGAAG TTGACCGAAG CAAGCTGCAG 
TGCCACAATG CGCAGCTGCG GACCGCCTTG GAGCAGGTGG AACGAGAACG GAGGAAGCTG 
AAGAGGGATT CCGTGCGTGC ATCCCGGGCA GGATCACTGG AGGCCAGAGA AACTATGACT 
TCATCCCCCA CACAGCAGGA TGGGAGAGGC AGTCAGAGGG GTTCCTCGGA CAGCGTGCTT 
GTGGTTGAGC TTCAGAGAGA GGTAGCCCTG CTGCGAGCCC AGCTGGCCTT GGAGCGAAAG 
CAGAGGCAGG ACTACATTGC ACGGTCCGTG CAGACCAGCC GTGAGCTAGC AGGCCTGCAT 
CACAGCCTGT CTCACTCCCT CCTCACCGTG GCCCAGGCCC CTGAGGCCAC TGTTCTGGAG 
GCTGAGACCC GAAAGCTGGA TGAGTCTCTG AATCAGAGTC TGACATCCCC AGGGCCATGT 
TTGCTACATC CCAGTCTGGA CACTACTCAA AATACCCACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032409.3
CDS	206..7450
Translation	METGSPGLNMKPQSLQLVLEGQVLALQQQMAENQAASWRKLKNSQEAQKRQATLVRKLQAKVLQYRSWCQDLEKRLEATGGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKLTVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	NM_008383.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008383.3

ORF Insert Sequence:

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCAGCA GGGAGCGGGA GCTGCTTCAG AAGGCCAGAG GAGAGCTGCA GCAGCAACTG 
GAAGTGCTGG AGCAGGAAGC GTGGCGACTG CGGAGGATGA ACATGGAGCT GCAGCTGCAG 
GGGGACTCAG CTCAAGGGGA GAAGCTGGAG CAGCAGGAGG AGTTGCACTT GGCTGTCCGG 
GAGAGGGAGC GCCTTCAGGA GACGCTGGTG GGCCTGGAAG CCAAGCAGTC AGAATCACTA 
AGTGAGCTGC TCACTCTTCG AGAAGCCCTG GAGTCAAGTC GCCTAGAAGG GGAGTTACTG 
AAGCAGGAGC GAGTGGAAGT GGCTGCAGCC TTGGCCAGGG CAGAACAGTC CATTGTGGAG 
CTGTCAGGTT CTGAGAACAG CCTGAAGGCA GAGGTAGCTG ATCTTCGAGC TGCAGCTGTC 
AAGCTTGGGG CCTTAAACGA GGCTTTGGCT TTAGATAAAG TTGAACTGAA CCAGCAGCTT 
CTCCAGTTGG AGCAGGAGAA CCAGTCTCTA TGCAGCAGAG TGGAGGCCGC AGAGCAGCTG 
AGAAGCGCTT TGCAGGTGGA CCTGGCGGAG GCAGAAAGGC GCAGGGAAGC CCTGTGGGAG 
AAGAAAACTC AGCTGGAGAC TCAGCTGCAG AAGGCAGAGG AAGCGGGGGC TGAGCTACAG 
GCAGAGCTGA GGGGCACCCG AGAAGAAAAG GAAGAGCTTA AAGATAAATT AAGTGAGGCA 
CATCACCAGC AAGAAACAGC TACAGCTCAC CTAGAACAGC TGCATCAGGA CGCAGAAAGA 
CAGGAAGAAA CACTTGCTAG GGCAGTCCAG GAGAAGGAAG CCCTAGTACG GGAGAGGGCA 
GCTCTTGAGG TGCGCCTGCA GGCTGTGGAG CGGGACCGAC AGGACCTCAC TGAACATGTC 
CTGGGGCTCA GGTCAGCCAA GGAGCAACTG GAGAGCAATC TATTTGAGGC CCAACAGCAA 
AATTCTGTGA TACAGGTCAC CAAGGGGCAG CTGGAGGTCC AGATTCAAAC TATTATCCAA 
GCCAAGGAAG TAATTCAAGG GGAAGTGAAA TGCCTGAAGC TGGAACTGGA TGCTGAGAGG 
ACCCGGGCAG AGCAGGAGTG GGATGCAGTA GCCAGGCAGC TAGCCCAAGC TGAGCAGGAG 
GGGCAAGCAT CTCTGGAGCG ACAGAAGGTG GCCCACGAGG AGGAGGTGAA CAGGCTGCAA 
GAGAAATGGG AGAAAGAGCG CTCCTGGCTG CAGCAGGAGC TGGATAAGAC CCTGGAAACC 
CTCGAGAGGG AGAGAGCAGA GCTGGAGACA AAGCTGAGAG AGCAGCAGAC AGAAATGGAA 
GCCATCCGTG CACAGAGGGA AGAGGAACGA AGCCAGGCTG ACAGCGCCCT GTATCAAATG 
CAGCTAGAAA CAGAGAAGGA GAGAGTATCC CTCCTGGAGA CACTGCTGAG GACCCAGAAG 
GAGCTGGCAG ATGCTAGTCA ACAGCTGGAA AGGCTGAGAC AGGACATGAA GATTCAGAAG 
TTAAAGGAAC AGGAGACCAC TGGCATGCTG CAGGCCCAGC TTCAGGAAAC ACAGCAGGAG 
CTGAAGGAGG CAGCCCAGCA GCACAGGGAC GACCTTGCTG CCTTCCAGAA GGACAAGCTG 
GACCTGCAGA AGCAGGTAGA GGACCTAATG TCTCAGCTGG TAGCCCACGA TGACTCCCAG 
AGGCTGGTGA AAGAAGAGAT TGAAGAGAAG GTGAAAGTGG CTCAAGAATG CAGCCGGATT 
CAGAAGGAAC TGGAGAAAGA GAACGCCAGC CTGGCTCTGT CGCTGGTGGA AAAGGAAAAA 
CGACTTCTCA TTTTACAAGA AGCTGACTCT GTAAGACAGC AGGAGCTGAG CTCCTTGCGC 
CAGGACATAC AGGAGGCCCA GGAAGGGCAA AGAGAGCTGG GTGTACAGGT GGAACTATTG 
AGGCAGGAGG TGAAGGAAAA GGAGGCTGAC TTTGTGGCCC GGGAAGCACA GCTGCTGGAG 
GAGTTGGAGG CCTCACGGGT TGCAGAGCAG CAACTTCGAG CGTCTTTGTG GGCCCAGGAA 
GCCAAGGCAA CCCAGCTGCA GCTGCAGCTG CGCAGCACTG AGAGCCAGCT AGAGGCATTG 
GTTGCAGAAC AACAGCCGGA GAACCAGGCA CAGGCACAGC TAGCTAGCCT CTGCTCTGTT 
CTGCAGCAGG CCCTGGGGTC TGCATGTGAG AGTAGGCCTG AGCTGAGGGG TGGAGGCGAC 
TCTGCTCCTA CCTTATGGGG CCCTGATCCA GATCAGAATG GAGCTTCCAG ACTCTTTAAG 
AGATGGTCGC TCCCAACGGC ACTGTCACCG GAGGCTGTGG CACTAGCTCT CCAGAAGCTC 
CATCAGGACG TGTGGAAGGC TCGGCAGGCC CGGGATGACC TAAGGGACCA GGTCCAGAAG 
CTGGTGCAGC GCCTGACTGA CACGGAGGCC CAGAAGAGCC AGGTTCACTC GGAGCTGCAG 
GACCTGCAGA GACAGCTCTC TCAGAGCCAG GAAGAGAAAT CCAAGTGGGA AGGGAGGCAG 
AACTCCCTGG AGTCTGAGCT GAGGGACTTG CACGAGACCG CGGCATCCTT ACAGAGTCGC 
CTCCGGCAGG CAGAACTGCA AAAAATGGAA GCTCAGAATG ATCGAGAGTT GCTTCAGGCA 
TCCAAGGAGA AGCTGTCAGC CCAGGTTGAA CATCTGCAAG CATGTGTTGC AGAAGCCCAG 
GCTCAGGCAG ATGCAGCTGC TGTCCTGGAA GAAGATCTGC GAACTGCTCG CTCTGCACTG 
AAACTGAAAA ACGAGGAGCT AGAGAGTGAA CGGGAGAGAG CCCAGGCTTT ACAAGAACAG 
GGTGAGCTGA AGGTGGCTCA GGGGAAAGCT TTGCAGGAGA ACCTAGCCCT GCTGGCACAG 
ACCCTGTCTA ACAGAGAGCG GGAGGTCGAG ACGTTGCAGG CTGAGGTTCA GGAACTGGAG 
AAGCAGCGGG AGATGCAGAA GGCTGCTCTG GAGCTGCTCT CCCTCGACCT GAAGAAGCGC 
AGTCGGGAGG TAGACCTGCA ACAAGAACAG ATTCAGGAGC TGGAGCAGTG CAGGTCAGTC 
TTAGAGCACT TGCCCATGGC CGTCCAAGAG CGAGAGCAGA AACTGAGTGT GCAGCGGGAC 
CAGATCAGGG AACTGGAGAA CGATCGGGAG GCCCAGAGGA GTGTCCTGGA ACACCAGCTT 
CTGGACCTGG AGCAGAAGGC TCAAGTGATT GAGTCCCAGA GGGGACAGAT TCAGGATCTG 
AAAAAGCAAC TGGGGACACT GGAATGCCTG GCCCTGGAAC TGGAGGAAAG CCATCACAAA 
GTGGAGAGCC AACAGAAGAT GATCACAGAA CTAGAGGGCC AGAGGGAAAT GCAGAGGGTG 
GCTCTAACCC ACCTCACACT GGACCTGGAA GAAAGGAGCC AGGAGCTGCA GGCACAAAGC 
AGCCAGCTCC ATGAGCTGGA GAACCATAGC ACCCATCTGG CAAAAGAGCT GCAGGAGAGG 
GACCAGGAGG TGACGTCCCA ACGCCAGCAG ATCGATGAGC TGCAGAAGCA GCAGGAACAG 
CTGGCTCAGG CCCTGGAGAG GAAAGGCCAG GAGCTGGTGC TGCAGAAGGA AAGGATTCAG 
GTTCTGGAAG ATCAGAGGAC GCTGCAGACC AAGATCTTAG AGGAGGACCT AGAACAGATC 
AAGCATTCCT TGAGAGAACG CAGCCAGGAG CTGGCCTCTC AGTGGCAGCT GGTGCATGAG 
CGTGCCGACG ATGGAAAGAG CCCGAGTAAA GGCCAGCGAG GGAGCCTGGA GCACTTGAAG 
CTGATCCTGC GTGATAAGGA GAAGGAGGTA GAGTGCCAGC AGGAGCGCAT CCAGGAACTT 
CAGGGCCACA TGGGCCAGCT GGAACAGCAG CTGCAGGGTC TGCACAGGAA GGTGGGGGAG 
ACTAGCCTGC TCCTGACCCA TCGAGAGCAG GAGACAGCAA CCCTGCAGCA GCATCTCCAG 
GAAGCCAAGG AACAAGGGGA ACTGAGAGAG CAGGTACTTC AGGGTCAGCT GGAGGAGGCC 
CAGAGAGATC TGGCCCAGAG GGACCATGAA CTAGAGACCC TCCGGCAGGA AAAACAGCAG 
ACCCAGGACC AGGAGGAAAG CATGAAGCTA AAGACAAGTG CTCTGCAGGC CGCTCTGGAG 
CAGGCCCATG CGACACTGAA GGAGCGTCAG GGGGAGCTGG AAGAACACAG GGAACAGGTG 
CGAAGGCTCC AGGAAGAACT AGAAGTGGAG GGACGGCAGG TACGGGCCCT GGAGGAGGTG 
TTGGGGGACC TAAGGGCTGA GTCTCGGGAG CACGAGAAGG CTGTGCTGGC CCTTCAACAA 
CGGTGTGCTG AGCAGGCCCA GGAACATGAG GCAGAAGCCA GGACCCTGCA GGACAGCTGG 
CTGCAGGCCC AGGCCACTCT CACAGAACAA GAACAAGAGC TAGCGGCACT GCGAGCGGAA 
AACCAGTATT CCCGCCGACA GGAAGAGGCT GCAGTTAGCC AGGCAGAGGC GCTGCAGGAG 
GCCCTCAGCA AAGCCCAGGC TGCCCTGCAG GAGAAAGAGC AGAGTCTGCT TGAACAGGCT 
GAGTTGAGCC ACACCCTGGA GGCCAGCACT GCCGCCTTGC AAGCTACCCT GGACACCTGC 
CAGGCAAGTG CCCGGCAGCT GGAGGAGGCC CTGAGGATTC GGGAAGGTGA GATCCAGGCC 
CAGGCTCTCC AACACCACGA GGTCACGCAG CACCTCCAGC AGGAACTTTG CCAGAAGAAA 
GAAGAGCTGA GGCAGCTACT AGAGAAGGCT GGGGCCCGGA GGAGTCAAGA AAATGGAATC 
CAAGAGAAGC AAAGTCTGGA GCAAGAGAGA CAAGAGGAAA CAAGGAGGCT CCTTGAGAGC 
CTAAAGGAGC TACAGCTGAC TGTAGCCCAA AGGGAAGAAG AGATTCTGAT GCTGAGGGAG 
GCCTCGTCCC CACGCCACAG AGCCCTCCCA GCCGAGAAGC CAGCTCTGCA ACCGCTCCCA 
GCACAACAGG AGTTAGAGCG GCTGCAGACT GCCCTAAGGC AAACAGAGGC CAGGGAGATT 
GAATGGAGGG AGAAGGCTCA GGACCTGGCT CTGTCCCTGG CCCAAAGCAA GGCCAGCATC 
AGCAGTCTGC AGGAGATAAC CATGTTCCTA CAAGCCTCTG TTCTAGAGCG GGAATCAGAA 
CAACAAAGAC TACAGGAGGA GTTGGTACTC AGCAGACAAG CTCTGGAAGA GCAGCAGTCA 
GGTGGCCCAC ACTCAACCAG CAGAGCAGAC CAGGGACCAA AAGTGGGACA GGGAAGCCAG 
TCTGGAGAGG TAGAGACTGA GCCTAGTCCT GGAGTGGAGG AGAAGGAACG GTTGACACAG 
AGGCTTGAAC GTCTGCAGCA AGCGGTGGCA GAGCTGGAAG TTGACCGAAG CAAGCTGCAG 
TGCCACAATG CGCAGCTGCG GACCGCCTTG GAGCAGGTGG AACGAGAACG GAGGAAGCTG 
AAGAGGGATT CCGTGCGTGC ATCCCGGGCA GGATCACTGG AGGCCAGAGA AACTATGACT 
TCATCCCCCA CACAGCAGGA TGGGAGAGGC AGTCAGAGGG GTTCCTCGGA CAGCGTGCTT 
GTGGTTGAGC TTCAGAGAGA GGTAGCCCTG CTGCGAGCCC AGCTGGCCTT GGAGCGAAAG 
CAGAGGCAGG ACTACATTGC ACGGTCCGTG CAGACCAGCC GTGAGCTAGC AGGCCTGCAT 
CACAGCCTGT CTCACTCCCT CCTCACCGTG GCCCAGGCCC CTGAGGCCAC TGTTCTGGAG 
GCTGAGACCC GAAAGCTGGA TGAGTCTCTG AATCAGAGTC TGACATCCCC AGGGCCATGT 
TTGCTACATC CCAGTCTGGA CACTACTCAA AATACCCACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( NM_001129999.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001123471.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant 3, mRNA.

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CloneID	OMu06035
Clone ID Related Accession (Same CDS sequence)	NM_001129999.1
Accession Version	NM_001129999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL833786.8 and AK045557.1. Transcript Variant: This variant (3) uses an alternate in-frame acceptor splice site at one of the coding exons, and includes an additional in-frame segment compared to variant 1, resulting in a longer isoform (2) compared to isoform 1. Sequence Note: Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGACAAA CCATGCAGAG 
TTAATGGAGC ATGAGGCATC TCTCAGTAGG AATGCTCAAG AGGAAAAGTT GTCTCTGCAG 
CAGGTGATCA AGGCTATAAC CCAGGCACTG GCCTCAGTGG AAGAAGAAGA CACTGTGACC 
CAGTCCTCAG GCCATGAGGA CTCGCTGCAG TCAGACTGTA ATGGCCTTTC CCAGTTTGAT 
CCCCAGGACC CAGACAGGGC TCTTACCCTG GTGCAGTCAG TGCTGACTCG GAGACAGCAG 
GCTGTGCAGG ACCTCAGGCA GCAGCTTTCA GGCTGCCAGG AGGCTATGAG CTTCTTGCAG 
CAGCAGCACG ATCAGTGGGA GGAAGAGGGC AGGGCCCTGA GGGAAAAGCT ACAGAAGCTC 
ACTGGGGAGC GGGATGCTCT GGCAGGCCAG ACTGTGGGCC TTCAGGGAGA GGTGGACTCT 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG ACCTTCAGGG AGAGGTGGAC 
TCTCTCAGCA GGGAGCGGGA GCTGCTTCAG AAGGCCAGAG GAGAGCTGCA GCAGCAACTG 
GAAGTGCTGG AGCAGGAAGC GTGGCGACTG CGGAGGATGA ACATGGAGCT GCAGCTGCAG 
GGGGACTCAG CTCAAGGGGA GAAGCTGGAG CAGCAGGAGG AGTTGCACTT GGCTGTCCGG 
GAGAGGGAGC GCCTTCAGGA GACGCTGGTG GGCCTGGAAG CCAAGCAGTC AGAATCACTA 
AGTGAGCTGC TCACTCTTCG AGAAGCCCTG GAGTCAAGTC GCCTAGAAGG GGAGTTACTG 
AAGCAGGAGC GAGTGGAAGT GGCTGCAGCC TTGGCCAGGG CAGAACAGTC CATTGTGGAG 
CTGTCAGGTT CTGAGAACAG CCTGAAGGCA GAGGTAGCTG ATCTTCGAGC TGCAGCTGTC 
AAGCTTGGGG CCTTAAACGA GGCTTTGGCT TTAGATAAAG TTGAACTGAA CCAGCAGCTT 
CTCCAGTTGG AGCAGGAGAA CCAGTCTCTA TGCAGCAGAG TGGAGGCCGC AGAGCAGCTG 
AGAAGCGCTT TGCAGGTGGA CCTGGCGGAG GCAGAAAGGC GCAGGGAAGC CCTGTGGGAG 
AAGAAAACTC AGCTGGAGAC TCAGCTGCAG AAGGCAGAGG AAGCGGGGGC TGAGCTACAG 
GCAGAGCTGA GGGGCACCCG AGAAGAAAAG GAAGAGCTTA AAGATAAATT AAGTGAGGCA 
CATCACCAGC AAGAAACAGC TACAGCTCAC CTAGAACAGC TGCATCAGGA CGCAGAAAGA 
CAGGAAGAAA CACTTGCTAG GGCAGTCCAG GAGAAGGAAG CCCTAGTACG GGAGAGGGCA 
GCTCTTGAGG TGCGCCTGCA GGCTGTGGAG CGGGACCGAC AGGACCTCAC TGAACATGTC 
CTGGGGCTCA GGTCAGCCAA GGAGCAACTG GAGAGCAATC TATTTGAGGC CCAACAGCAA 
AATTCTGTGA TACAGGTCAC CAAGGGGCAG CTGGAGGTCC AGATTCAAAC TATTATCCAA 
GCCAAGGAAG TAATTCAAGG GGAAGTGAAA TGCCTGAAGC TGGAACTGGA TGCTGAGAGG 
ACCCGGGCAG AGCAGGAGTG GGATGCAGTA GCCAGGCAGC TAGCCCAAGC TGAGCAGGAG 
GGGCAAGCAT CTCTGGAGCG ACAGAAGGTG GCCCACGAGG AGGAGGTGAA CAGGCTGCAA 
GAGAAATGGG AGAAAGAGCG CTCCTGGCTG CAGCAGGAGC TGGATAAGAC CCTGGAAACC 
CTCGAGAGGG AGAGAGCAGA GCTGGAGACA AAGCTGAGAG AGCAGCAGAC AGAAATGGAA 
GCCATCCGTG CACAGAGGGA AGAGGAACGA AGCCAGGCTG ACAGCGCCCT GTATCAAATG 
CAGCTAGAAA CAGAGAAGGA GAGAGTATCC CTCCTGGAGA CACTGCTGAG GACCCAGAAG 
GAGCTGGCAG ATGCTAGTCA ACAGCTGGAA AGGCTGAGAC AGGACATGAA GATTCAGAAG 
TTAAAGGAAC AGGAGACCAC TGGCATGCTG CAGGCCCAGC TTCAGGAAAC ACAGCAGGAG 
CTGAAGGAGG CAGCCCAGCA GCACAGGGAC GACCTTGCTG CCTTCCAGAA GGACAAGCTG 
GACCTGCAGA AGCAGGTAGA GGACCTAATG TCTCAGCTGG TAGCCCACGA TGACTCCCAG 
AGGCTGGTGA AAGAAGAGAT TGAAGAGAAG GTGAAAGTGG CTCAAGAATG CAGCCGGATT 
CAGAAGGAAC TGGAGAAAGA GAACGCCAGC CTGGCTCTGT CGCTGGTGGA AAAGGAAAAA 
CGACTTCTCA TTTTACAAGA AGCTGACTCT GTAAGACAGC AGGAGCTGAG CTCCTTGCGC 
CAGGACATAC AGGAGGCCCA GGAAGGGCAA AGAGAGCTGG GTGTACAGGT GGAACTATTG 
AGGCAGGAGG TGAAGGAAAA GGAGGCTGAC TTTGTGGCCC GGGAAGCACA GCTGCTGGAG 
GAGTTGGAGG CCTCACGGGT TGCAGAGCAG CAACTTCGAG CGTCTTTGTG GGCCCAGGAA 
GCCAAGGCAA CCCAGCTGCA GCTGCAGCTG CGCAGCACTG AGAGCCAGCT AGAGGCATTG 
GTTGCAGAAC AACAGCCGGA GAACCAGGCA CAGGCACAGC TAGCTAGCCT CTGCTCTGTT 
CTGCAGCAGG CCCTGGGGTC TGCATGTGAG AGTAGGCCTG AGCTGAGGGG TGGAGGCGAC 
TCTGCTCCTA CCTTATGGGG CCCTGATCCA GATCAGAATG GAGCTTCCAG ACTCTTTAAG 
AGATGGTCGC TCCCAACGGC ACTGTCACCG GAGGCTGTGG CACTAGCTCT CCAGAAGCTC 
CATCAGGACG TGTGGAAGGC TCGGCAGGCC CGGGATGACC TAAGGGACCA GGTCCAGAAG 
CTGGTGCAGC GCCTGACTGA CACGGAGGCC CAGAAGAGCC AGGTTCACTC GGAGCTGCAG 
GACCTGCAGA GACAGCTCTC TCAGAGCCAG GAAGAGAAAT CCAAGTGGGA AGGGAGGCAG 
AACTCCCTGG AGTCTGAGCT GAGGGACTTG CACGAGACCG CGGCATCCTT ACAGAGTCGC 
CTCCGGCAGG CAGAACTGCA AAAAATGGAA GCTCAGAATG ATCGAGAGTT GCTTCAGGCA 
TCCAAGGAGA AGCTGTCAGC CCAGGTTGAA CATCTGCAAG CATGTGTTGC AGAAGCCCAG 
GCTCAGGCAG ATGCAGCTGC TGTCCTGGAA GAAGATCTGC GAACTGCTCG CTCTGCACTG 
AAACTGAAAA ACGAGGAGCT AGAGAGTGAA CGGGAGAGAG CCCAGGCTTT ACAAGAACAG 
GGTGAGCTGA AGGTGGCTCA GGGGAAAGCT TTGCAGGAGA ACCTAGCCCT GCTGGCACAG 
ACCCTGTCTA ACAGAGAGCG GGAGGTCGAG ACGTTGCAGG CTGAGGTTCA GGAACTGGAG 
AAGCAGCGGG AGATGCAGAA GGCTGCTCTG GAGCTGCTCT CCCTCGACCT GAAGAAGCGC 
AGTCGGGAGG TAGACCTGCA ACAAGAACAG ATTCAGGAGC TGGAGCAGTG CAGGTCAGTC 
TTAGAGCACT TGCCCATGGC CGTCCAAGAG CGAGAGCAGA AACTGAGTGT GCAGCGGGAC 
CAGATCAGGG AACTGGAGAA CGATCGGGAG GCCCAGAGGA GTGTCCTGGA ACACCAGCTT 
CTGGACCTGG AGCAGAAGGC TCAAGTGATT GAGTCCCAGA GGGGACAGAT TCAGGATCTG 
AAAAAGCAAC TGGGGACACT GGAATGCCTG GCCCTGGAAC TGGAGGAAAG CCATCACAAA 
GTGGAGAGCC AACAGAAGAT GATCACAGAA CTAGAGGGCC AGAGGGAAAT GCAGAGGGTG 
GCTCTAACCC ACCTCACACT GGACCTGGAA GAAAGGAGCC AGGAGCTGCA GGCACAAAGC 
AGCCAGCTCC ATGAGCTGGA GAACCATAGC ACCCATCTGG CAAAAGAGCT GCAGGAGAGG 
GACCAGGAGG TGACGTCCCA ACGCCAGCAG ATCGATGAGC TGCAGAAGCA GCAGGAACAG 
CTGGCTCAGG CCCTGGAGAG GAAAGGCCAG GAGCTGGTGC TGCAGAAGGA AAGGATTCAG 
GTTCTGGAAG ATCAGAGGAC GCTGCAGACC AAGATCTTAG AGGAGGACCT AGAACAGATC 
AAGCATTCCT TGAGAGAACG CAGCCAGGAG CTGGCCTCTC AGTGGCAGCT GGTGCATGAG 
CGTGCCGACG ATGGAAAGAG CCCGAGTAAA GGCCAGCGAG GGAGCCTGGA GCACTTGAAG 
CTGATCCTGC GTGATAAGGA GAAGGAGGTA GAGTGCCAGC AGGAGCGCAT CCAGGAACTT 
CAGGGCCACA TGGGCCAGCT GGAACAGCAG CTGCAGGGTC TGCACAGGAA GGTGGGGGAG 
ACTAGCCTGC TCCTGACCCA TCGAGAGCAG GAGACAGCAA CCCTGCAGCA GCATCTCCAG 
GAAGCCAAGG AACAAGGGGA ACTGAGAGAG CAGGTACTTC AGGGTCAGCT GGAGGAGGCC 
CAGAGAGATC TGGCCCAGAG GGACCATGAA CTAGAGACCC TCCGGCAGGA AAAACAGCAG 
ACCCAGGACC AGGAGGAAAG CATGAAGCTA AAGACAAGTG CTCTGCAGGC CGCTCTGGAG 
CAGGCCCATG CGACACTGAA GGAGCGTCAG GGGGAGCTGG AAGAACACAG GGAACAGGTG 
CGAAGGCTCC AGGAAGAACT AGAAGTGGAG GGACGGCAGG TACGGGCCCT GGAGGAGGTG 
TTGGGGGACC TAAGGGCTGA GTCTCGGGAG CACGAGAAGG CTGTGCTGGC CCTTCAACAA 
CGGTGTGCTG AGCAGGCCCA GGAACATGAG GCAGAAGCCA GGACCCTGCA GGACAGCTGG 
CTGCAGGCCC AGGCCACTCT CACAGAACAA GAACAAGAGC TAGCGGCACT GCGAGCGGAA 
AACCAGTATT CCCGCCGACA GGAAGAGGCT GCAGTTAGCC AGGCAGAGGC GCTGCAGGAG 
GCCCTCAGCA AAGCCCAGGC TGCCCTGCAG GAGAAAGAGC AGAGTCTGCT TGAACAGGCT 
GAGTTGAGCC ACACCCTGGA GGCCAGCACT GCCGCCTTGC AAGCTACCCT GGACACCTGC 
CAGGCAAGTG CCCGGCAGCT GGAGGAGGCC CTGAGGATTC GGGAAGGTGA GATCCAGGCC 
CAGGCTCTCC AACACCACGA GGTCACGCAG CACCTCCAGC AGGAACTTTG CCAGAAGAAA 
GAAGAGCTGA GGCAGCTACT AGAGAAGGCT GGGGCCCGGA GGAGTCAAGA AAATGGAATC 
CAAGAGAAGC AAAGTCTGGA GCAAGAGAGA CAAGAGGAAA CAAGGAGGCT CCTTGAGAGC 
CTAAAGGAGC TACAGCTGAC TGTAGCCCAA AGGGAAGAAG AGATTCTGAT GCTGAGGGAG 
GCCTCGTCCC CACGCCACAG AGCCCTCCCA GCCGAGAAGC CAGCTCTGCA ACCGCTCCCA 
GCACAACAGG AGTTAGAGCG GCTGCAGACT GCCCTAAGGC AAACAGAGGC CAGGGAGATT 
GAATGGAGGG AGAAGGCTCA GGACCTGGCT CTGTCCCTGG CCCAAAGCAA GGCCAGCATC 
AGCAGTCTGC AGGAGATAAC CATGTTCCTA CAAGCCTCTG TTCTAGAGCG GGAATCAGAA 
CAACAAAGAC TACAGGAGGA GTTGGTACTC AGCAGACAAG CTCTGGAAGA GCAGCAGTCA 
GGTGGCCCAC ACTCAACCAG CAGAGCAGAC CAGGGACCAA AAGTGGGACA GGGAAGCCAG 
TCTGGAGAGG TAGAGACTGA GCCTAGTCCT GGAGTGGAGG AGAAGGAACG GTTGACACAG 
AGGCTTGAAC GTCTGCAGCA AGCGGTGGCA GAGCTGGAAG TTGACCGAAG CAAGCTGCAG 
TGCCACAATG CGCAGCTGCG GACCGCCTTG GAGCAGGTGG AACGAGAACG GAGGAAGCTG 
AAGAGGGATT CCGTGCGTGC ATCCCGGGCA GGATCACTGG AGGCCAGAGA AACTATGACT 
TCATCCCCCA CACAGCAGGA TGGGAGAGGC AGTCAGAGGG GTTCCTCGGA CAGCGTGCTT 
GTGGTTGAGC TTCAGAGAGA GGTAGCCCTG CTGCGAGCCC AGCTGGCCTT GGAGCGAAAG 
CAGAGGCAGG ACTACATTGC ACGGTCCGTG CAGACCAGCC GTGAGCTAGC AGGCCTGCAT 
CACAGCCTGT CTCACTCCCT CCTCACCGTG GCCCAGGCCC CTGAGGCCAC TGTTCTGGAG 
GCTGAGACCC GAAAGCTGGA TGAGTCTCTG AATCAGAGTC TGACATCCCC AGGGCCATGT 
TTGCTACATC CCAGTCTGGA CACTACTCAA AATACCCACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123471.1
CDS	206..7510
Translation	METGSPGLNMKPQSLQLVLEGQVLALQQQMAENQAASWRKLKNSQEAQKRQATLVRKLQAKVLQYRSWCQDLEKRLEATGGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKLTVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILTNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	NM_001129999.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129999.1

ORF Insert Sequence:

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGACAAA CCATGCAGAG 
TTAATGGAGC ATGAGGCATC TCTCAGTAGG AATGCTCAAG AGGAAAAGTT GTCTCTGCAG 
CAGGTGATCA AGGCTATAAC CCAGGCACTG GCCTCAGTGG AAGAAGAAGA CACTGTGACC 
CAGTCCTCAG GCCATGAGGA CTCGCTGCAG TCAGACTGTA ATGGCCTTTC CCAGTTTGAT 
CCCCAGGACC CAGACAGGGC TCTTACCCTG GTGCAGTCAG TGCTGACTCG GAGACAGCAG 
GCTGTGCAGG ACCTCAGGCA GCAGCTTTCA GGCTGCCAGG AGGCTATGAG CTTCTTGCAG 
CAGCAGCACG ATCAGTGGGA GGAAGAGGGC AGGGCCCTGA GGGAAAAGCT ACAGAAGCTC 
ACTGGGGAGC GGGATGCTCT GGCAGGCCAG ACTGTGGGCC TTCAGGGAGA GGTGGACTCT 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG ACCTTCAGGG AGAGGTGGAC 
TCTCTCAGCA GGGAGCGGGA GCTGCTTCAG AAGGCCAGAG GAGAGCTGCA GCAGCAACTG 
GAAGTGCTGG AGCAGGAAGC GTGGCGACTG CGGAGGATGA ACATGGAGCT GCAGCTGCAG 
GGGGACTCAG CTCAAGGGGA GAAGCTGGAG CAGCAGGAGG AGTTGCACTT GGCTGTCCGG 
GAGAGGGAGC GCCTTCAGGA GACGCTGGTG GGCCTGGAAG CCAAGCAGTC AGAATCACTA 
AGTGAGCTGC TCACTCTTCG AGAAGCCCTG GAGTCAAGTC GCCTAGAAGG GGAGTTACTG 
AAGCAGGAGC GAGTGGAAGT GGCTGCAGCC TTGGCCAGGG CAGAACAGTC CATTGTGGAG 
CTGTCAGGTT CTGAGAACAG CCTGAAGGCA GAGGTAGCTG ATCTTCGAGC TGCAGCTGTC 
AAGCTTGGGG CCTTAAACGA GGCTTTGGCT TTAGATAAAG TTGAACTGAA CCAGCAGCTT 
CTCCAGTTGG AGCAGGAGAA CCAGTCTCTA TGCAGCAGAG TGGAGGCCGC AGAGCAGCTG 
AGAAGCGCTT TGCAGGTGGA CCTGGCGGAG GCAGAAAGGC GCAGGGAAGC CCTGTGGGAG 
AAGAAAACTC AGCTGGAGAC TCAGCTGCAG AAGGCAGAGG AAGCGGGGGC TGAGCTACAG 
GCAGAGCTGA GGGGCACCCG AGAAGAAAAG GAAGAGCTTA AAGATAAATT AAGTGAGGCA 
CATCACCAGC AAGAAACAGC TACAGCTCAC CTAGAACAGC TGCATCAGGA CGCAGAAAGA 
CAGGAAGAAA CACTTGCTAG GGCAGTCCAG GAGAAGGAAG CCCTAGTACG GGAGAGGGCA 
GCTCTTGAGG TGCGCCTGCA GGCTGTGGAG CGGGACCGAC AGGACCTCAC TGAACATGTC 
CTGGGGCTCA GGTCAGCCAA GGAGCAACTG GAGAGCAATC TATTTGAGGC CCAACAGCAA 
AATTCTGTGA TACAGGTCAC CAAGGGGCAG CTGGAGGTCC AGATTCAAAC TATTATCCAA 
GCCAAGGAAG TAATTCAAGG GGAAGTGAAA TGCCTGAAGC TGGAACTGGA TGCTGAGAGG 
ACCCGGGCAG AGCAGGAGTG GGATGCAGTA GCCAGGCAGC TAGCCCAAGC TGAGCAGGAG 
GGGCAAGCAT CTCTGGAGCG ACAGAAGGTG GCCCACGAGG AGGAGGTGAA CAGGCTGCAA 
GAGAAATGGG AGAAAGAGCG CTCCTGGCTG CAGCAGGAGC TGGATAAGAC CCTGGAAACC 
CTCGAGAGGG AGAGAGCAGA GCTGGAGACA AAGCTGAGAG AGCAGCAGAC AGAAATGGAA 
GCCATCCGTG CACAGAGGGA AGAGGAACGA AGCCAGGCTG ACAGCGCCCT GTATCAAATG 
CAGCTAGAAA CAGAGAAGGA GAGAGTATCC CTCCTGGAGA CACTGCTGAG GACCCAGAAG 
GAGCTGGCAG ATGCTAGTCA ACAGCTGGAA AGGCTGAGAC AGGACATGAA GATTCAGAAG 
TTAAAGGAAC AGGAGACCAC TGGCATGCTG CAGGCCCAGC TTCAGGAAAC ACAGCAGGAG 
CTGAAGGAGG CAGCCCAGCA GCACAGGGAC GACCTTGCTG CCTTCCAGAA GGACAAGCTG 
GACCTGCAGA AGCAGGTAGA GGACCTAATG TCTCAGCTGG TAGCCCACGA TGACTCCCAG 
AGGCTGGTGA AAGAAGAGAT TGAAGAGAAG GTGAAAGTGG CTCAAGAATG CAGCCGGATT 
CAGAAGGAAC TGGAGAAAGA GAACGCCAGC CTGGCTCTGT CGCTGGTGGA AAAGGAAAAA 
CGACTTCTCA TTTTACAAGA AGCTGACTCT GTAAGACAGC AGGAGCTGAG CTCCTTGCGC 
CAGGACATAC AGGAGGCCCA GGAAGGGCAA AGAGAGCTGG GTGTACAGGT GGAACTATTG 
AGGCAGGAGG TGAAGGAAAA GGAGGCTGAC TTTGTGGCCC GGGAAGCACA GCTGCTGGAG 
GAGTTGGAGG CCTCACGGGT TGCAGAGCAG CAACTTCGAG CGTCTTTGTG GGCCCAGGAA 
GCCAAGGCAA CCCAGCTGCA GCTGCAGCTG CGCAGCACTG AGAGCCAGCT AGAGGCATTG 
GTTGCAGAAC AACAGCCGGA GAACCAGGCA CAGGCACAGC TAGCTAGCCT CTGCTCTGTT 
CTGCAGCAGG CCCTGGGGTC TGCATGTGAG AGTAGGCCTG AGCTGAGGGG TGGAGGCGAC 
TCTGCTCCTA CCTTATGGGG CCCTGATCCA GATCAGAATG GAGCTTCCAG ACTCTTTAAG 
AGATGGTCGC TCCCAACGGC ACTGTCACCG GAGGCTGTGG CACTAGCTCT CCAGAAGCTC 
CATCAGGACG TGTGGAAGGC TCGGCAGGCC CGGGATGACC TAAGGGACCA GGTCCAGAAG 
CTGGTGCAGC GCCTGACTGA CACGGAGGCC CAGAAGAGCC AGGTTCACTC GGAGCTGCAG 
GACCTGCAGA GACAGCTCTC TCAGAGCCAG GAAGAGAAAT CCAAGTGGGA AGGGAGGCAG 
AACTCCCTGG AGTCTGAGCT GAGGGACTTG CACGAGACCG CGGCATCCTT ACAGAGTCGC 
CTCCGGCAGG CAGAACTGCA AAAAATGGAA GCTCAGAATG ATCGAGAGTT GCTTCAGGCA 
TCCAAGGAGA AGCTGTCAGC CCAGGTTGAA CATCTGCAAG CATGTGTTGC AGAAGCCCAG 
GCTCAGGCAG ATGCAGCTGC TGTCCTGGAA GAAGATCTGC GAACTGCTCG CTCTGCACTG 
AAACTGAAAA ACGAGGAGCT AGAGAGTGAA CGGGAGAGAG CCCAGGCTTT ACAAGAACAG 
GGTGAGCTGA AGGTGGCTCA GGGGAAAGCT TTGCAGGAGA ACCTAGCCCT GCTGGCACAG 
ACCCTGTCTA ACAGAGAGCG GGAGGTCGAG ACGTTGCAGG CTGAGGTTCA GGAACTGGAG 
AAGCAGCGGG AGATGCAGAA GGCTGCTCTG GAGCTGCTCT CCCTCGACCT GAAGAAGCGC 
AGTCGGGAGG TAGACCTGCA ACAAGAACAG ATTCAGGAGC TGGAGCAGTG CAGGTCAGTC 
TTAGAGCACT TGCCCATGGC CGTCCAAGAG CGAGAGCAGA AACTGAGTGT GCAGCGGGAC 
CAGATCAGGG AACTGGAGAA CGATCGGGAG GCCCAGAGGA GTGTCCTGGA ACACCAGCTT 
CTGGACCTGG AGCAGAAGGC TCAAGTGATT GAGTCCCAGA GGGGACAGAT TCAGGATCTG 
AAAAAGCAAC TGGGGACACT GGAATGCCTG GCCCTGGAAC TGGAGGAAAG CCATCACAAA 
GTGGAGAGCC AACAGAAGAT GATCACAGAA CTAGAGGGCC AGAGGGAAAT GCAGAGGGTG 
GCTCTAACCC ACCTCACACT GGACCTGGAA GAAAGGAGCC AGGAGCTGCA GGCACAAAGC 
AGCCAGCTCC ATGAGCTGGA GAACCATAGC ACCCATCTGG CAAAAGAGCT GCAGGAGAGG 
GACCAGGAGG TGACGTCCCA ACGCCAGCAG ATCGATGAGC TGCAGAAGCA GCAGGAACAG 
CTGGCTCAGG CCCTGGAGAG GAAAGGCCAG GAGCTGGTGC TGCAGAAGGA AAGGATTCAG 
GTTCTGGAAG ATCAGAGGAC GCTGCAGACC AAGATCTTAG AGGAGGACCT AGAACAGATC 
AAGCATTCCT TGAGAGAACG CAGCCAGGAG CTGGCCTCTC AGTGGCAGCT GGTGCATGAG 
CGTGCCGACG ATGGAAAGAG CCCGAGTAAA GGCCAGCGAG GGAGCCTGGA GCACTTGAAG 
CTGATCCTGC GTGATAAGGA GAAGGAGGTA GAGTGCCAGC AGGAGCGCAT CCAGGAACTT 
CAGGGCCACA TGGGCCAGCT GGAACAGCAG CTGCAGGGTC TGCACAGGAA GGTGGGGGAG 
ACTAGCCTGC TCCTGACCCA TCGAGAGCAG GAGACAGCAA CCCTGCAGCA GCATCTCCAG 
GAAGCCAAGG AACAAGGGGA ACTGAGAGAG CAGGTACTTC AGGGTCAGCT GGAGGAGGCC 
CAGAGAGATC TGGCCCAGAG GGACCATGAA CTAGAGACCC TCCGGCAGGA AAAACAGCAG 
ACCCAGGACC AGGAGGAAAG CATGAAGCTA AAGACAAGTG CTCTGCAGGC CGCTCTGGAG 
CAGGCCCATG CGACACTGAA GGAGCGTCAG GGGGAGCTGG AAGAACACAG GGAACAGGTG 
CGAAGGCTCC AGGAAGAACT AGAAGTGGAG GGACGGCAGG TACGGGCCCT GGAGGAGGTG 
TTGGGGGACC TAAGGGCTGA GTCTCGGGAG CACGAGAAGG CTGTGCTGGC CCTTCAACAA 
CGGTGTGCTG AGCAGGCCCA GGAACATGAG GCAGAAGCCA GGACCCTGCA GGACAGCTGG 
CTGCAGGCCC AGGCCACTCT CACAGAACAA GAACAAGAGC TAGCGGCACT GCGAGCGGAA 
AACCAGTATT CCCGCCGACA GGAAGAGGCT GCAGTTAGCC AGGCAGAGGC GCTGCAGGAG 
GCCCTCAGCA AAGCCCAGGC TGCCCTGCAG GAGAAAGAGC AGAGTCTGCT TGAACAGGCT 
GAGTTGAGCC ACACCCTGGA GGCCAGCACT GCCGCCTTGC AAGCTACCCT GGACACCTGC 
CAGGCAAGTG CCCGGCAGCT GGAGGAGGCC CTGAGGATTC GGGAAGGTGA GATCCAGGCC 
CAGGCTCTCC AACACCACGA GGTCACGCAG CACCTCCAGC AGGAACTTTG CCAGAAGAAA 
GAAGAGCTGA GGCAGCTACT AGAGAAGGCT GGGGCCCGGA GGAGTCAAGA AAATGGAATC 
CAAGAGAAGC AAAGTCTGGA GCAAGAGAGA CAAGAGGAAA CAAGGAGGCT CCTTGAGAGC 
CTAAAGGAGC TACAGCTGAC TGTAGCCCAA AGGGAAGAAG AGATTCTGAT GCTGAGGGAG 
GCCTCGTCCC CACGCCACAG AGCCCTCCCA GCCGAGAAGC CAGCTCTGCA ACCGCTCCCA 
GCACAACAGG AGTTAGAGCG GCTGCAGACT GCCCTAAGGC AAACAGAGGC CAGGGAGATT 
GAATGGAGGG AGAAGGCTCA GGACCTGGCT CTGTCCCTGG CCCAAAGCAA GGCCAGCATC 
AGCAGTCTGC AGGAGATAAC CATGTTCCTA CAAGCCTCTG TTCTAGAGCG GGAATCAGAA 
CAACAAAGAC TACAGGAGGA GTTGGTACTC AGCAGACAAG CTCTGGAAGA GCAGCAGTCA 
GGTGGCCCAC ACTCAACCAG CAGAGCAGAC CAGGGACCAA AAGTGGGACA GGGAAGCCAG 
TCTGGAGAGG TAGAGACTGA GCCTAGTCCT GGAGTGGAGG AGAAGGAACG GTTGACACAG 
AGGCTTGAAC GTCTGCAGCA AGCGGTGGCA GAGCTGGAAG TTGACCGAAG CAAGCTGCAG 
TGCCACAATG CGCAGCTGCG GACCGCCTTG GAGCAGGTGG AACGAGAACG GAGGAAGCTG 
AAGAGGGATT CCGTGCGTGC ATCCCGGGCA GGATCACTGG AGGCCAGAGA AACTATGACT 
TCATCCCCCA CACAGCAGGA TGGGAGAGGC AGTCAGAGGG GTTCCTCGGA CAGCGTGCTT 
GTGGTTGAGC TTCAGAGAGA GGTAGCCCTG CTGCGAGCCC AGCTGGCCTT GGAGCGAAAG 
CAGAGGCAGG ACTACATTGC ACGGTCCGTG CAGACCAGCC GTGAGCTAGC AGGCCTGCAT 
CACAGCCTGT CTCACTCCCT CCTCACCGTG GCCCAGGCCC CTGAGGCCAC TGTTCTGGAG 
GCTGAGACCC GAAAGCTGGA TGAGTCTCTG AATCAGAGTC TGACATCCCC AGGGCCATGT 
TTGCTACATC CCAGTCTGGA CACTACTCAA AATACCCACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( NM_001130000.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001123472.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant 4, mRNA.

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CloneID	OMu06037
Clone ID Related Accession (Same CDS sequence)	NM_001130000.1 , XM_006498796.1 , XM_006498797.3 , XM_006498797.4
Accession Version	NM_001130000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK145562.1 and AL833786.8. Transcript Variant: This variant (4) differs in the 5' UTR and includes an additional in-frame segment compared to variant 1, resulting in a longer isoform (3) compared to isoform 1. Sequence Note: Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGACCTTCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG GGAGCTGCTT CAGAAGGCCA GAGGAGAGCT GCAGCAGCAA 
CTGGAAGTGC TGGAGCAGGA AGCGTGGCGA CTGCGGAGGA TGAACATGGA GCTGCAGCTG 
CAGGGGGACT CAGCTCAAGG GGAGAAGCTG GAGCAGCAGG AGGAGTTGCA CTTGGCTGTC 
CGGGAGAGGG AGCGCCTTCA GGAGACGCTG GTGGGCCTGG AAGCCAAGCA GTCAGAATCA 
CTAAGTGAGC TGCTCACTCT TCGAGAAGCC CTGGAGTCAA GTCGCCTAGA AGGGGAGTTA 
CTGAAGCAGG AGCGAGTGGA AGTGGCTGCA GCCTTGGCCA GGGCAGAACA GTCCATTGTG 
GAGCTGTCAG GTTCTGAGAA CAGCCTGAAG GCAGAGGTAG CTGATCTTCG AGCTGCAGCT 
GTCAAGCTTG GGGCCTTAAA CGAGGCTTTG GCTTTAGATA AAGTTGAACT GAACCAGCAG 
CTTCTCCAGT TGGAGCAGGA GAACCAGTCT CTATGCAGCA GAGTGGAGGC CGCAGAGCAG 
CTGAGAAGCG CTTTGCAGGT GGACCTGGCG GAGGCAGAAA GGCGCAGGGA AGCCCTGTGG 
GAGAAGAAAA CTCAGCTGGA GACTCAGCTG CAGAAGGCAG AGGAAGCGGG GGCTGAGCTA 
CAGGCAGAGC TGAGGGGCAC CCGAGAAGAA AAGGAAGAGC TTAAAGATAA ATTAAGTGAG 
GCACATCACC AGCAAGAAAC AGCTACAGCT CACCTAGAAC AGCTGCATCA GGACGCAGAA 
AGACAGGAAG AAACACTTGC TAGGGCAGTC CAGGAGAAGG AAGCCCTAGT ACGGGAGAGG 
GCAGCTCTTG AGGTGCGCCT GCAGGCTGTG GAGCGGGACC GACAGGACCT CACTGAACAT 
GTCCTGGGGC TCAGGTCAGC CAAGGAGCAA CTGGAGAGCA ATCTATTTGA GGCCCAACAG 
CAAAATTCTG TGATACAGGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATTATC 
CAAGCCAAGG AAGTAATTCA AGGGGAAGTG AAATGCCTGA AGCTGGAACT GGATGCTGAG 
AGGACCCGGG CAGAGCAGGA GTGGGATGCA GTAGCCAGGC AGCTAGCCCA AGCTGAGCAG 
GAGGGGCAAG CATCTCTGGA GCGACAGAAG GTGGCCCACG AGGAGGAGGT GAACAGGCTG 
CAAGAGAAAT GGGAGAAAGA GCGCTCCTGG CTGCAGCAGG AGCTGGATAA GACCCTGGAA 
ACCCTCGAGA GGGAGAGAGC AGAGCTGGAG ACAAAGCTGA GAGAGCAGCA GACAGAAATG 
GAAGCCATCC GTGCACAGAG GGAAGAGGAA CGAAGCCAGG CTGACAGCGC CCTGTATCAA 
ATGCAGCTAG AAACAGAGAA GGAGAGAGTA TCCCTCCTGG AGACACTGCT GAGGACCCAG 
AAGGAGCTGG CAGATGCTAG TCAACAGCTG GAAAGGCTGA GACAGGACAT GAAGATTCAG 
AAGTTAAAGG AACAGGAGAC CACTGGCATG CTGCAGGCCC AGCTTCAGGA AACACAGCAG 
GAGCTGAAGG AGGCAGCCCA GCAGCACAGG GACGACCTTG CTGCCTTCCA GAAGGACAAG 
CTGGACCTGC AGAAGCAGGT AGAGGACCTA ATGTCTCAGC TGGTAGCCCA CGATGACTCC 
CAGAGGCTGG TGAAAGAAGA GATTGAAGAG AAGGTGAAAG TGGCTCAAGA ATGCAGCCGG 
ATTCAGAAGG AACTGGAGAA AGAGAACGCC AGCCTGGCTC TGTCGCTGGT GGAAAAGGAA 
AAACGACTTC TCATTTTACA AGAAGCTGAC TCTGTAAGAC AGCAGGAGCT GAGCTCCTTG 
CGCCAGGACA TACAGGAGGC CCAGGAAGGG CAAAGAGAGC TGGGTGTACA GGTGGAACTA 
TTGAGGCAGG AGGTGAAGGA AAAGGAGGCT GACTTTGTGG CCCGGGAAGC ACAGCTGCTG 
GAGGAGTTGG AGGCCTCACG GGTTGCAGAG CAGCAACTTC GAGCGTCTTT GTGGGCCCAG 
GAAGCCAAGG CAACCCAGCT GCAGCTGCAG CTGCGCAGCA CTGAGAGCCA GCTAGAGGCA 
TTGGTTGCAG AACAACAGCC GGAGAACCAG GCACAGGCAC AGCTAGCTAG CCTCTGCTCT 
GTTCTGCAGC AGGCCCTGGG GTCTGCATGT GAGAGTAGGC CTGAGCTGAG GGGTGGAGGC 
GACTCTGCTC CTACCTTATG GGGCCCTGAT CCAGATCAGA ATGGAGCTTC CAGACTCTTT 
AAGAGATGGT CGCTCCCAAC GGCACTGTCA CCGGAGGCTG TGGCACTAGC TCTCCAGAAG 
CTCCATCAGG ACGTGTGGAA GGCTCGGCAG GCCCGGGATG ACCTAAGGGA CCAGGTCCAG 
AAGCTGGTGC AGCGCCTGAC TGACACGGAG GCCCAGAAGA GCCAGGTTCA CTCGGAGCTG 
CAGGACCTGC AGAGACAGCT CTCTCAGAGC CAGGAAGAGA AATCCAAGTG GGAAGGGAGG 
CAGAACTCCC TGGAGTCTGA GCTGAGGGAC TTGCACGAGA CCGCGGCATC CTTACAGAGT 
CGCCTCCGGC AGGCAGAACT GCAAAAAATG GAAGCTCAGA ATGATCGAGA GTTGCTTCAG 
GCATCCAAGG AGAAGCTGTC AGCCCAGGTT GAACATCTGC AAGCATGTGT TGCAGAAGCC 
CAGGCTCAGG CAGATGCAGC TGCTGTCCTG GAAGAAGATC TGCGAACTGC TCGCTCTGCA 
CTGAAACTGA AAAACGAGGA GCTAGAGAGT GAACGGGAGA GAGCCCAGGC TTTACAAGAA 
CAGGGTGAGC TGAAGGTGGC TCAGGGGAAA GCTTTGCAGG AGAACCTAGC CCTGCTGGCA 
CAGACCCTGT CTAACAGAGA GCGGGAGGTC GAGACGTTGC AGGCTGAGGT TCAGGAACTG 
GAGAAGCAGC GGGAGATGCA GAAGGCTGCT CTGGAGCTGC TCTCCCTCGA CCTGAAGAAG 
CGCAGTCGGG AGGTAGACCT GCAACAAGAA CAGATTCAGG AGCTGGAGCA GTGCAGGTCA 
GTCTTAGAGC ACTTGCCCAT GGCCGTCCAA GAGCGAGAGC AGAAACTGAG TGTGCAGCGG 
GACCAGATCA GGGAACTGGA GAACGATCGG GAGGCCCAGA GGAGTGTCCT GGAACACCAG 
CTTCTGGACC TGGAGCAGAA GGCTCAAGTG ATTGAGTCCC AGAGGGGACA GATTCAGGAT 
CTGAAAAAGC AACTGGGGAC ACTGGAATGC CTGGCCCTGG AACTGGAGGA AAGCCATCAC 
AAAGTGGAGA GCCAACAGAA GATGATCACA GAACTAGAGG GCCAGAGGGA AATGCAGAGG 
GTGGCTCTAA CCCACCTCAC ACTGGACCTG GAAGAAAGGA GCCAGGAGCT GCAGGCACAA 
AGCAGCCAGC TCCATGAGCT GGAGAACCAT AGCACCCATC TGGCAAAAGA GCTGCAGGAG 
AGGGACCAGG AGGTGACGTC CCAACGCCAG CAGATCGATG AGCTGCAGAA GCAGCAGGAA 
CAGCTGGCTC AGGCCCTGGA GAGGAAAGGC CAGGAGCTGG TGCTGCAGAA GGAAAGGATT 
CAGGTTCTGG AAGATCAGAG GACGCTGCAG ACCAAGATCT TAGAGGAGGA CCTAGAACAG 
ATCAAGCATT CCTTGAGAGA ACGCAGCCAG GAGCTGGCCT CTCAGTGGCA GCTGGTGCAT 
GAGCGTGCCG ACGATGGAAA GAGCCCGAGT AAAGGCCAGC GAGGGAGCCT GGAGCACTTG 
AAGCTGATCC TGCGTGATAA GGAGAAGGAG GTAGAGTGCC AGCAGGAGCG CATCCAGGAA 
CTTCAGGGCC ACATGGGCCA GCTGGAACAG CAGCTGCAGG GTCTGCACAG GAAGGTGGGG 
GAGACTAGCC TGCTCCTGAC CCATCGAGAG CAGGAGACAG CAACCCTGCA GCAGCATCTC 
CAGGAAGCCA AGGAACAAGG GGAACTGAGA GAGCAGGTAC TTCAGGGTCA GCTGGAGGAG 
GCCCAGAGAG ATCTGGCCCA GAGGGACCAT GAACTAGAGA CCCTCCGGCA GGAAAAACAG 
CAGACCCAGG ACCAGGAGGA AAGCATGAAG CTAAAGACAA GTGCTCTGCA GGCCGCTCTG 
GAGCAGGCCC ATGCGACACT GAAGGAGCGT CAGGGGGAGC TGGAAGAACA CAGGGAACAG 
GTGCGAAGGC TCCAGGAAGA ACTAGAAGTG GAGGGACGGC AGGTACGGGC CCTGGAGGAG 
GTGTTGGGGG ACCTAAGGGC TGAGTCTCGG GAGCACGAGA AGGCTGTGCT GGCCCTTCAA 
CAACGGTGTG CTGAGCAGGC CCAGGAACAT GAGGCAGAAG CCAGGACCCT GCAGGACAGC 
TGGCTGCAGG CCCAGGCCAC TCTCACAGAA CAAGAACAAG AGCTAGCGGC ACTGCGAGCG 
GAAAACCAGT ATTCCCGCCG ACAGGAAGAG GCTGCAGTTA GCCAGGCAGA GGCGCTGCAG 
GAGGCCCTCA GCAAAGCCCA GGCTGCCCTG CAGGAGAAAG AGCAGAGTCT GCTTGAACAG 
GCTGAGTTGA GCCACACCCT GGAGGCCAGC ACTGCCGCCT TGCAAGCTAC CCTGGACACC 
TGCCAGGCAA GTGCCCGGCA GCTGGAGGAG GCCCTGAGGA TTCGGGAAGG TGAGATCCAG 
GCCCAGGCTC TCCAACACCA CGAGGTCACG CAGCACCTCC AGCAGGAACT TTGCCAGAAG 
AAAGAAGAGC TGAGGCAGCT ACTAGAGAAG GCTGGGGCCC GGAGGAGTCA AGAAAATGGA 
ATCCAAGAGA AGCAAAGTCT GGAGCAAGAG AGACAAGAGG AAACAAGGAG GCTCCTTGAG 
AGCCTAAAGG AGCTACAGCT GACTGTAGCC CAAAGGGAAG AAGAGATTCT GATGCTGAGG 
GAGGCCTCGT CCCCACGCCA CAGAGCCCTC CCAGCCGAGA AGCCAGCTCT GCAACCGCTC 
CCAGCACAAC AGGAGTTAGA GCGGCTGCAG ACTGCCCTAA GGCAAACAGA GGCCAGGGAG 
ATTGAATGGA GGGAGAAGGC TCAGGACCTG GCTCTGTCCC TGGCCCAAAG CAAGGCCAGC 
ATCAGCAGTC TGCAGGAGAT AACCATGTTC CTACAAGCCT CTGTTCTAGA GCGGGAATCA 
GAACAACAAA GACTACAGGA GGAGTTGGTA CTCAGCAGAC AAGCTCTGGA AGAGCAGCAG 
TCAGGTGGCC CACACTCAAC CAGCAGAGCA GACCAGGGAC CAAAAGTGGG ACAGGGAAGC 
CAGTCTGGAG AGGTAGAGAC TGAGCCTAGT CCTGGAGTGG AGGAGAAGGA ACGGTTGACA 
CAGAGGCTTG AACGTCTGCA GCAAGCGGTG GCAGAGCTGG AAGTTGACCG AAGCAAGCTG 
CAGTGCCACA ATGCGCAGCT GCGGACCGCC TTGGAGCAGG TGGAACGAGA ACGGAGGAAG 
CTGAAGAGGG ATTCCGTGCG TGCATCCCGG GCAGGATCAC TGGAGGCCAG AGAAACTATG 
ACTTCATCCC CCACACAGCA GGATGGGAGA GGCAGTCAGA GGGGTTCCTC GGACAGCGTG 
CTTGTGGTTG AGCTTCAGAG AGAGGTAGCC CTGCTGCGAG CCCAGCTGGC CTTGGAGCGA 
AAGCAGAGGC AGGACTACAT TGCACGGTCC GTGCAGACCA GCCGTGAGCT AGCAGGCCTG 
CATCACAGCC TGTCTCACTC CCTCCTCACC GTGGCCCAGG CCCCTGAGGC CACTGTTCTG 
GAGGCTGAGA CCCGAAAGCT GGATGAGTCT CTGAATCAGA GTCTGACATC CCCAGGGCCA 
TGTTTGCTAC ATCCCAGTCT GGACACTACT CAAAATACCC ACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123472.1
CDS	201..7508
Translation	METGSPGLNMKPQSLQLVLEGQVLALQQQMAENQAASWRKLKNSQEAQKRQATLVRKLQAKVLQYRSWCQDLEKRLEATGGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKLTVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	NM_001130000.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130000.1

ORF Insert Sequence:

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGACCTTCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG GGAGCTGCTT CAGAAGGCCA GAGGAGAGCT GCAGCAGCAA 
CTGGAAGTGC TGGAGCAGGA AGCGTGGCGA CTGCGGAGGA TGAACATGGA GCTGCAGCTG 
CAGGGGGACT CAGCTCAAGG GGAGAAGCTG GAGCAGCAGG AGGAGTTGCA CTTGGCTGTC 
CGGGAGAGGG AGCGCCTTCA GGAGACGCTG GTGGGCCTGG AAGCCAAGCA GTCAGAATCA 
CTAAGTGAGC TGCTCACTCT TCGAGAAGCC CTGGAGTCAA GTCGCCTAGA AGGGGAGTTA 
CTGAAGCAGG AGCGAGTGGA AGTGGCTGCA GCCTTGGCCA GGGCAGAACA GTCCATTGTG 
GAGCTGTCAG GTTCTGAGAA CAGCCTGAAG GCAGAGGTAG CTGATCTTCG AGCTGCAGCT 
GTCAAGCTTG GGGCCTTAAA CGAGGCTTTG GCTTTAGATA AAGTTGAACT GAACCAGCAG 
CTTCTCCAGT TGGAGCAGGA GAACCAGTCT CTATGCAGCA GAGTGGAGGC CGCAGAGCAG 
CTGAGAAGCG CTTTGCAGGT GGACCTGGCG GAGGCAGAAA GGCGCAGGGA AGCCCTGTGG 
GAGAAGAAAA CTCAGCTGGA GACTCAGCTG CAGAAGGCAG AGGAAGCGGG GGCTGAGCTA 
CAGGCAGAGC TGAGGGGCAC CCGAGAAGAA AAGGAAGAGC TTAAAGATAA ATTAAGTGAG 
GCACATCACC AGCAAGAAAC AGCTACAGCT CACCTAGAAC AGCTGCATCA GGACGCAGAA 
AGACAGGAAG AAACACTTGC TAGGGCAGTC CAGGAGAAGG AAGCCCTAGT ACGGGAGAGG 
GCAGCTCTTG AGGTGCGCCT GCAGGCTGTG GAGCGGGACC GACAGGACCT CACTGAACAT 
GTCCTGGGGC TCAGGTCAGC CAAGGAGCAA CTGGAGAGCA ATCTATTTGA GGCCCAACAG 
CAAAATTCTG TGATACAGGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATTATC 
CAAGCCAAGG AAGTAATTCA AGGGGAAGTG AAATGCCTGA AGCTGGAACT GGATGCTGAG 
AGGACCCGGG CAGAGCAGGA GTGGGATGCA GTAGCCAGGC AGCTAGCCCA AGCTGAGCAG 
GAGGGGCAAG CATCTCTGGA GCGACAGAAG GTGGCCCACG AGGAGGAGGT GAACAGGCTG 
CAAGAGAAAT GGGAGAAAGA GCGCTCCTGG CTGCAGCAGG AGCTGGATAA GACCCTGGAA 
ACCCTCGAGA GGGAGAGAGC AGAGCTGGAG ACAAAGCTGA GAGAGCAGCA GACAGAAATG 
GAAGCCATCC GTGCACAGAG GGAAGAGGAA CGAAGCCAGG CTGACAGCGC CCTGTATCAA 
ATGCAGCTAG AAACAGAGAA GGAGAGAGTA TCCCTCCTGG AGACACTGCT GAGGACCCAG 
AAGGAGCTGG CAGATGCTAG TCAACAGCTG GAAAGGCTGA GACAGGACAT GAAGATTCAG 
AAGTTAAAGG AACAGGAGAC CACTGGCATG CTGCAGGCCC AGCTTCAGGA AACACAGCAG 
GAGCTGAAGG AGGCAGCCCA GCAGCACAGG GACGACCTTG CTGCCTTCCA GAAGGACAAG 
CTGGACCTGC AGAAGCAGGT AGAGGACCTA ATGTCTCAGC TGGTAGCCCA CGATGACTCC 
CAGAGGCTGG TGAAAGAAGA GATTGAAGAG AAGGTGAAAG TGGCTCAAGA ATGCAGCCGG 
ATTCAGAAGG AACTGGAGAA AGAGAACGCC AGCCTGGCTC TGTCGCTGGT GGAAAAGGAA 
AAACGACTTC TCATTTTACA AGAAGCTGAC TCTGTAAGAC AGCAGGAGCT GAGCTCCTTG 
CGCCAGGACA TACAGGAGGC CCAGGAAGGG CAAAGAGAGC TGGGTGTACA GGTGGAACTA 
TTGAGGCAGG AGGTGAAGGA AAAGGAGGCT GACTTTGTGG CCCGGGAAGC ACAGCTGCTG 
GAGGAGTTGG AGGCCTCACG GGTTGCAGAG CAGCAACTTC GAGCGTCTTT GTGGGCCCAG 
GAAGCCAAGG CAACCCAGCT GCAGCTGCAG CTGCGCAGCA CTGAGAGCCA GCTAGAGGCA 
TTGGTTGCAG AACAACAGCC GGAGAACCAG GCACAGGCAC AGCTAGCTAG CCTCTGCTCT 
GTTCTGCAGC AGGCCCTGGG GTCTGCATGT GAGAGTAGGC CTGAGCTGAG GGGTGGAGGC 
GACTCTGCTC CTACCTTATG GGGCCCTGAT CCAGATCAGA ATGGAGCTTC CAGACTCTTT 
AAGAGATGGT CGCTCCCAAC GGCACTGTCA CCGGAGGCTG TGGCACTAGC TCTCCAGAAG 
CTCCATCAGG ACGTGTGGAA GGCTCGGCAG GCCCGGGATG ACCTAAGGGA CCAGGTCCAG 
AAGCTGGTGC AGCGCCTGAC TGACACGGAG GCCCAGAAGA GCCAGGTTCA CTCGGAGCTG 
CAGGACCTGC AGAGACAGCT CTCTCAGAGC CAGGAAGAGA AATCCAAGTG GGAAGGGAGG 
CAGAACTCCC TGGAGTCTGA GCTGAGGGAC TTGCACGAGA CCGCGGCATC CTTACAGAGT 
CGCCTCCGGC AGGCAGAACT GCAAAAAATG GAAGCTCAGA ATGATCGAGA GTTGCTTCAG 
GCATCCAAGG AGAAGCTGTC AGCCCAGGTT GAACATCTGC AAGCATGTGT TGCAGAAGCC 
CAGGCTCAGG CAGATGCAGC TGCTGTCCTG GAAGAAGATC TGCGAACTGC TCGCTCTGCA 
CTGAAACTGA AAAACGAGGA GCTAGAGAGT GAACGGGAGA GAGCCCAGGC TTTACAAGAA 
CAGGGTGAGC TGAAGGTGGC TCAGGGGAAA GCTTTGCAGG AGAACCTAGC CCTGCTGGCA 
CAGACCCTGT CTAACAGAGA GCGGGAGGTC GAGACGTTGC AGGCTGAGGT TCAGGAACTG 
GAGAAGCAGC GGGAGATGCA GAAGGCTGCT CTGGAGCTGC TCTCCCTCGA CCTGAAGAAG 
CGCAGTCGGG AGGTAGACCT GCAACAAGAA CAGATTCAGG AGCTGGAGCA GTGCAGGTCA 
GTCTTAGAGC ACTTGCCCAT GGCCGTCCAA GAGCGAGAGC AGAAACTGAG TGTGCAGCGG 
GACCAGATCA GGGAACTGGA GAACGATCGG GAGGCCCAGA GGAGTGTCCT GGAACACCAG 
CTTCTGGACC TGGAGCAGAA GGCTCAAGTG ATTGAGTCCC AGAGGGGACA GATTCAGGAT 
CTGAAAAAGC AACTGGGGAC ACTGGAATGC CTGGCCCTGG AACTGGAGGA AAGCCATCAC 
AAAGTGGAGA GCCAACAGAA GATGATCACA GAACTAGAGG GCCAGAGGGA AATGCAGAGG 
GTGGCTCTAA CCCACCTCAC ACTGGACCTG GAAGAAAGGA GCCAGGAGCT GCAGGCACAA 
AGCAGCCAGC TCCATGAGCT GGAGAACCAT AGCACCCATC TGGCAAAAGA GCTGCAGGAG 
AGGGACCAGG AGGTGACGTC CCAACGCCAG CAGATCGATG AGCTGCAGAA GCAGCAGGAA 
CAGCTGGCTC AGGCCCTGGA GAGGAAAGGC CAGGAGCTGG TGCTGCAGAA GGAAAGGATT 
CAGGTTCTGG AAGATCAGAG GACGCTGCAG ACCAAGATCT TAGAGGAGGA CCTAGAACAG 
ATCAAGCATT CCTTGAGAGA ACGCAGCCAG GAGCTGGCCT CTCAGTGGCA GCTGGTGCAT 
GAGCGTGCCG ACGATGGAAA GAGCCCGAGT AAAGGCCAGC GAGGGAGCCT GGAGCACTTG 
AAGCTGATCC TGCGTGATAA GGAGAAGGAG GTAGAGTGCC AGCAGGAGCG CATCCAGGAA 
CTTCAGGGCC ACATGGGCCA GCTGGAACAG CAGCTGCAGG GTCTGCACAG GAAGGTGGGG 
GAGACTAGCC TGCTCCTGAC CCATCGAGAG CAGGAGACAG CAACCCTGCA GCAGCATCTC 
CAGGAAGCCA AGGAACAAGG GGAACTGAGA GAGCAGGTAC TTCAGGGTCA GCTGGAGGAG 
GCCCAGAGAG ATCTGGCCCA GAGGGACCAT GAACTAGAGA CCCTCCGGCA GGAAAAACAG 
CAGACCCAGG ACCAGGAGGA AAGCATGAAG CTAAAGACAA GTGCTCTGCA GGCCGCTCTG 
GAGCAGGCCC ATGCGACACT GAAGGAGCGT CAGGGGGAGC TGGAAGAACA CAGGGAACAG 
GTGCGAAGGC TCCAGGAAGA ACTAGAAGTG GAGGGACGGC AGGTACGGGC CCTGGAGGAG 
GTGTTGGGGG ACCTAAGGGC TGAGTCTCGG GAGCACGAGA AGGCTGTGCT GGCCCTTCAA 
CAACGGTGTG CTGAGCAGGC CCAGGAACAT GAGGCAGAAG CCAGGACCCT GCAGGACAGC 
TGGCTGCAGG CCCAGGCCAC TCTCACAGAA CAAGAACAAG AGCTAGCGGC ACTGCGAGCG 
GAAAACCAGT ATTCCCGCCG ACAGGAAGAG GCTGCAGTTA GCCAGGCAGA GGCGCTGCAG 
GAGGCCCTCA GCAAAGCCCA GGCTGCCCTG CAGGAGAAAG AGCAGAGTCT GCTTGAACAG 
GCTGAGTTGA GCCACACCCT GGAGGCCAGC ACTGCCGCCT TGCAAGCTAC CCTGGACACC 
TGCCAGGCAA GTGCCCGGCA GCTGGAGGAG GCCCTGAGGA TTCGGGAAGG TGAGATCCAG 
GCCCAGGCTC TCCAACACCA CGAGGTCACG CAGCACCTCC AGCAGGAACT TTGCCAGAAG 
AAAGAAGAGC TGAGGCAGCT ACTAGAGAAG GCTGGGGCCC GGAGGAGTCA AGAAAATGGA 
ATCCAAGAGA AGCAAAGTCT GGAGCAAGAG AGACAAGAGG AAACAAGGAG GCTCCTTGAG 
AGCCTAAAGG AGCTACAGCT GACTGTAGCC CAAAGGGAAG AAGAGATTCT GATGCTGAGG 
GAGGCCTCGT CCCCACGCCA CAGAGCCCTC CCAGCCGAGA AGCCAGCTCT GCAACCGCTC 
CCAGCACAAC AGGAGTTAGA GCGGCTGCAG ACTGCCCTAA GGCAAACAGA GGCCAGGGAG 
ATTGAATGGA GGGAGAAGGC TCAGGACCTG GCTCTGTCCC TGGCCCAAAG CAAGGCCAGC 
ATCAGCAGTC TGCAGGAGAT AACCATGTTC CTACAAGCCT CTGTTCTAGA GCGGGAATCA 
GAACAACAAA GACTACAGGA GGAGTTGGTA CTCAGCAGAC AAGCTCTGGA AGAGCAGCAG 
TCAGGTGGCC CACACTCAAC CAGCAGAGCA GACCAGGGAC CAAAAGTGGG ACAGGGAAGC 
CAGTCTGGAG AGGTAGAGAC TGAGCCTAGT CCTGGAGTGG AGGAGAAGGA ACGGTTGACA 
CAGAGGCTTG AACGTCTGCA GCAAGCGGTG GCAGAGCTGG AAGTTGACCG AAGCAAGCTG 
CAGTGCCACA ATGCGCAGCT GCGGACCGCC TTGGAGCAGG TGGAACGAGA ACGGAGGAAG 
CTGAAGAGGG ATTCCGTGCG TGCATCCCGG GCAGGATCAC TGGAGGCCAG AGAAACTATG 
ACTTCATCCC CCACACAGCA GGATGGGAGA GGCAGTCAGA GGGGTTCCTC GGACAGCGTG 
CTTGTGGTTG AGCTTCAGAG AGAGGTAGCC CTGCTGCGAG CCCAGCTGGC CTTGGAGCGA 
AAGCAGAGGC AGGACTACAT TGCACGGTCC GTGCAGACCA GCCGTGAGCT AGCAGGCCTG 
CATCACAGCC TGTCTCACTC CCTCCTCACC GTGGCCCAGG CCCCTGAGGC CACTGTTCTG 
GAGGCTGAGA CCCGAAAGCT GGATGAGTCT CTGAATCAGA GTCTGACATC CCCAGGGCCA 
TGTTTGCTAC ATCCCAGTCT GGACACTACT CAAAATACCC ACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( XM_006498796.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498859.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant X1, mRNA.

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CloneID	OMu06037
Clone ID Related Accession (Same CDS sequence)	NM_001130000.1 , XM_006498796.1 , XM_006498797.3 , XM_006498797.4
Accession Version	XM_006498796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGACCTTCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG GGAGCTGCTT CAGAAGGCCA GAGGAGAGCT GCAGCAGCAA 
CTGGAAGTGC TGGAGCAGGA AGCGTGGCGA CTGCGGAGGA TGAACATGGA GCTGCAGCTG 
CAGGGGGACT CAGCTCAAGG GGAGAAGCTG GAGCAGCAGG AGGAGTTGCA CTTGGCTGTC 
CGGGAGAGGG AGCGCCTTCA GGAGACGCTG GTGGGCCTGG AAGCCAAGCA GTCAGAATCA 
CTAAGTGAGC TGCTCACTCT TCGAGAAGCC CTGGAGTCAA GTCGCCTAGA AGGGGAGTTA 
CTGAAGCAGG AGCGAGTGGA AGTGGCTGCA GCCTTGGCCA GGGCAGAACA GTCCATTGTG 
GAGCTGTCAG GTTCTGAGAA CAGCCTGAAG GCAGAGGTAG CTGATCTTCG AGCTGCAGCT 
GTCAAGCTTG GGGCCTTAAA CGAGGCTTTG GCTTTAGATA AAGTTGAACT GAACCAGCAG 
CTTCTCCAGT TGGAGCAGGA GAACCAGTCT CTATGCAGCA GAGTGGAGGC CGCAGAGCAG 
CTGAGAAGCG CTTTGCAGGT GGACCTGGCG GAGGCAGAAA GGCGCAGGGA AGCCCTGTGG 
GAGAAGAAAA CTCAGCTGGA GACTCAGCTG CAGAAGGCAG AGGAAGCGGG GGCTGAGCTA 
CAGGCAGAGC TGAGGGGCAC CCGAGAAGAA AAGGAAGAGC TTAAAGATAA ATTAAGTGAG 
GCACATCACC AGCAAGAAAC AGCTACAGCT CACCTAGAAC AGCTGCATCA GGACGCAGAA 
AGACAGGAAG AAACACTTGC TAGGGCAGTC CAGGAGAAGG AAGCCCTAGT ACGGGAGAGG 
GCAGCTCTTG AGGTGCGCCT GCAGGCTGTG GAGCGGGACC GACAGGACCT CACTGAACAT 
GTCCTGGGGC TCAGGTCAGC CAAGGAGCAA CTGGAGAGCA ATCTATTTGA GGCCCAACAG 
CAAAATTCTG TGATACAGGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATTATC 
CAAGCCAAGG AAGTAATTCA AGGGGAAGTG AAATGCCTGA AGCTGGAACT GGATGCTGAG 
AGGACCCGGG CAGAGCAGGA GTGGGATGCA GTAGCCAGGC AGCTAGCCCA AGCTGAGCAG 
GAGGGGCAAG CATCTCTGGA GCGACAGAAG GTGGCCCACG AGGAGGAGGT GAACAGGCTG 
CAAGAGAAAT GGGAGAAAGA GCGCTCCTGG CTGCAGCAGG AGCTGGATAA GACCCTGGAA 
ACCCTCGAGA GGGAGAGAGC AGAGCTGGAG ACAAAGCTGA GAGAGCAGCA GACAGAAATG 
GAAGCCATCC GTGCACAGAG GGAAGAGGAA CGAAGCCAGG CTGACAGCGC CCTGTATCAA 
ATGCAGCTAG AAACAGAGAA GGAGAGAGTA TCCCTCCTGG AGACACTGCT GAGGACCCAG 
AAGGAGCTGG CAGATGCTAG TCAACAGCTG GAAAGGCTGA GACAGGACAT GAAGATTCAG 
AAGTTAAAGG AACAGGAGAC CACTGGCATG CTGCAGGCCC AGCTTCAGGA AACACAGCAG 
GAGCTGAAGG AGGCAGCCCA GCAGCACAGG GACGACCTTG CTGCCTTCCA GAAGGACAAG 
CTGGACCTGC AGAAGCAGGT AGAGGACCTA ATGTCTCAGC TGGTAGCCCA CGATGACTCC 
CAGAGGCTGG TGAAAGAAGA GATTGAAGAG AAGGTGAAAG TGGCTCAAGA ATGCAGCCGG 
ATTCAGAAGG AACTGGAGAA AGAGAACGCC AGCCTGGCTC TGTCGCTGGT GGAAAAGGAA 
AAACGACTTC TCATTTTACA AGAAGCTGAC TCTGTAAGAC AGCAGGAGCT GAGCTCCTTG 
CGCCAGGACA TACAGGAGGC CCAGGAAGGG CAAAGAGAGC TGGGTGTACA GGTGGAACTA 
TTGAGGCAGG AGGTGAAGGA AAAGGAGGCT GACTTTGTGG CCCGGGAAGC ACAGCTGCTG 
GAGGAGTTGG AGGCCTCACG GGTTGCAGAG CAGCAACTTC GAGCGTCTTT GTGGGCCCAG 
GAAGCCAAGG CAACCCAGCT GCAGCTGCAG CTGCGCAGCA CTGAGAGCCA GCTAGAGGCA 
TTGGTTGCAG AACAACAGCC GGAGAACCAG GCACAGGCAC AGCTAGCTAG CCTCTGCTCT 
GTTCTGCAGC AGGCCCTGGG GTCTGCATGT GAGAGTAGGC CTGAGCTGAG GGGTGGAGGC 
GACTCTGCTC CTACCTTATG GGGCCCTGAT CCAGATCAGA ATGGAGCTTC CAGACTCTTT 
AAGAGATGGT CGCTCCCAAC GGCACTGTCA CCGGAGGCTG TGGCACTAGC TCTCCAGAAG 
CTCCATCAGG ACGTGTGGAA GGCTCGGCAG GCCCGGGATG ACCTAAGGGA CCAGGTCCAG 
AAGCTGGTGC AGCGCCTGAC TGACACGGAG GCCCAGAAGA GCCAGGTTCA CTCGGAGCTG 
CAGGACCTGC AGAGACAGCT CTCTCAGAGC CAGGAAGAGA AATCCAAGTG GGAAGGGAGG 
CAGAACTCCC TGGAGTCTGA GCTGAGGGAC TTGCACGAGA CCGCGGCATC CTTACAGAGT 
CGCCTCCGGC AGGCAGAACT GCAAAAAATG GAAGCTCAGA ATGATCGAGA GTTGCTTCAG 
GCATCCAAGG AGAAGCTGTC AGCCCAGGTT GAACATCTGC AAGCATGTGT TGCAGAAGCC 
CAGGCTCAGG CAGATGCAGC TGCTGTCCTG GAAGAAGATC TGCGAACTGC TCGCTCTGCA 
CTGAAACTGA AAAACGAGGA GCTAGAGAGT GAACGGGAGA GAGCCCAGGC TTTACAAGAA 
CAGGGTGAGC TGAAGGTGGC TCAGGGGAAA GCTTTGCAGG AGAACCTAGC CCTGCTGGCA 
CAGACCCTGT CTAACAGAGA GCGGGAGGTC GAGACGTTGC AGGCTGAGGT TCAGGAACTG 
GAGAAGCAGC GGGAGATGCA GAAGGCTGCT CTGGAGCTGC TCTCCCTCGA CCTGAAGAAG 
CGCAGTCGGG AGGTAGACCT GCAACAAGAA CAGATTCAGG AGCTGGAGCA GTGCAGGTCA 
GTCTTAGAGC ACTTGCCCAT GGCCGTCCAA GAGCGAGAGC AGAAACTGAG TGTGCAGCGG 
GACCAGATCA GGGAACTGGA GAACGATCGG GAGGCCCAGA GGAGTGTCCT GGAACACCAG 
CTTCTGGACC TGGAGCAGAA GGCTCAAGTG ATTGAGTCCC AGAGGGGACA GATTCAGGAT 
CTGAAAAAGC AACTGGGGAC ACTGGAATGC CTGGCCCTGG AACTGGAGGA AAGCCATCAC 
AAAGTGGAGA GCCAACAGAA GATGATCACA GAACTAGAGG GCCAGAGGGA AATGCAGAGG 
GTGGCTCTAA CCCACCTCAC ACTGGACCTG GAAGAAAGGA GCCAGGAGCT GCAGGCACAA 
AGCAGCCAGC TCCATGAGCT GGAGAACCAT AGCACCCATC TGGCAAAAGA GCTGCAGGAG 
AGGGACCAGG AGGTGACGTC CCAACGCCAG CAGATCGATG AGCTGCAGAA GCAGCAGGAA 
CAGCTGGCTC AGGCCCTGGA GAGGAAAGGC CAGGAGCTGG TGCTGCAGAA GGAAAGGATT 
CAGGTTCTGG AAGATCAGAG GACGCTGCAG ACCAAGATCT TAGAGGAGGA CCTAGAACAG 
ATCAAGCATT CCTTGAGAGA ACGCAGCCAG GAGCTGGCCT CTCAGTGGCA GCTGGTGCAT 
GAGCGTGCCG ACGATGGAAA GAGCCCGAGT AAAGGCCAGC GAGGGAGCCT GGAGCACTTG 
AAGCTGATCC TGCGTGATAA GGAGAAGGAG GTAGAGTGCC AGCAGGAGCG CATCCAGGAA 
CTTCAGGGCC ACATGGGCCA GCTGGAACAG CAGCTGCAGG GTCTGCACAG GAAGGTGGGG 
GAGACTAGCC TGCTCCTGAC CCATCGAGAG CAGGAGACAG CAACCCTGCA GCAGCATCTC 
CAGGAAGCCA AGGAACAAGG GGAACTGAGA GAGCAGGTAC TTCAGGGTCA GCTGGAGGAG 
GCCCAGAGAG ATCTGGCCCA GAGGGACCAT GAACTAGAGA CCCTCCGGCA GGAAAAACAG 
CAGACCCAGG ACCAGGAGGA AAGCATGAAG CTAAAGACAA GTGCTCTGCA GGCCGCTCTG 
GAGCAGGCCC ATGCGACACT GAAGGAGCGT CAGGGGGAGC TGGAAGAACA CAGGGAACAG 
GTGCGAAGGC TCCAGGAAGA ACTAGAAGTG GAGGGACGGC AGGTACGGGC CCTGGAGGAG 
GTGTTGGGGG ACCTAAGGGC TGAGTCTCGG GAGCACGAGA AGGCTGTGCT GGCCCTTCAA 
CAACGGTGTG CTGAGCAGGC CCAGGAACAT GAGGCAGAAG CCAGGACCCT GCAGGACAGC 
TGGCTGCAGG CCCAGGCCAC TCTCACAGAA CAAGAACAAG AGCTAGCGGC ACTGCGAGCG 
GAAAACCAGT ATTCCCGCCG ACAGGAAGAG GCTGCAGTTA GCCAGGCAGA GGCGCTGCAG 
GAGGCCCTCA GCAAAGCCCA GGCTGCCCTG CAGGAGAAAG AGCAGAGTCT GCTTGAACAG 
GCTGAGTTGA GCCACACCCT GGAGGCCAGC ACTGCCGCCT TGCAAGCTAC CCTGGACACC 
TGCCAGGCAA GTGCCCGGCA GCTGGAGGAG GCCCTGAGGA TTCGGGAAGG TGAGATCCAG 
GCCCAGGCTC TCCAACACCA CGAGGTCACG CAGCACCTCC AGCAGGAACT TTGCCAGAAG 
AAAGAAGAGC TGAGGCAGCT ACTAGAGAAG GCTGGGGCCC GGAGGAGTCA AGAAAATGGA 
ATCCAAGAGA AGCAAAGTCT GGAGCAAGAG AGACAAGAGG AAACAAGGAG GCTCCTTGAG 
AGCCTAAAGG AGCTACAGCT GACTGTAGCC CAAAGGGAAG AAGAGATTCT GATGCTGAGG 
GAGGCCTCGT CCCCACGCCA CAGAGCCCTC CCAGCCGAGA AGCCAGCTCT GCAACCGCTC 
CCAGCACAAC AGGAGTTAGA GCGGCTGCAG ACTGCCCTAA GGCAAACAGA GGCCAGGGAG 
ATTGAATGGA GGGAGAAGGC TCAGGACCTG GCTCTGTCCC TGGCCCAAAG CAAGGCCAGC 
ATCAGCAGTC TGCAGGAGAT AACCATGTTC CTACAAGCCT CTGTTCTAGA GCGGGAATCA 
GAACAACAAA GACTACAGGA GGAGTTGGTA CTCAGCAGAC AAGCTCTGGA AGAGCAGCAG 
TCAGGTGGCC CACACTCAAC CAGCAGAGCA GACCAGGGAC CAAAAGTGGG ACAGGGAAGC 
CAGTCTGGAG AGGTAGAGAC TGAGCCTAGT CCTGGAGTGG AGGAGAAGGA ACGGTTGACA 
CAGAGGCTTG AACGTCTGCA GCAAGCGGTG GCAGAGCTGG AAGTTGACCG AAGCAAGCTG 
CAGTGCCACA ATGCGCAGCT GCGGACCGCC TTGGAGCAGG TGGAACGAGA ACGGAGGAAG 
CTGAAGAGGG ATTCCGTGCG TGCATCCCGG GCAGGATCAC TGGAGGCCAG AGAAACTATG 
ACTTCATCCC CCACACAGCA GGATGGGAGA GGCAGTCAGA GGGGTTCCTC GGACAGCGTG 
CTTGTGGTTG AGCTTCAGAG AGAGGTAGCC CTGCTGCGAG CCCAGCTGGC CTTGGAGCGA 
AAGCAGAGGC AGGACTACAT TGCACGGTCC GTGCAGACCA GCCGTGAGCT AGCAGGCCTG 
CATCACAGCC TGTCTCACTC CCTCCTCACC GTGGCCCAGG CCCCTGAGGC CACTGTTCTG 
GAGGCTGAGA CCCGAAAGCT GGATGAGTCT CTGAATCAGA GTCTGACATC CCCAGGGCCA 
TGTTTGCTAC ATCCCAGTCT GGACACTACT CAAAATACCC ACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498859.1
CDS	301..7608
Translation	METGSPGLNMKPQSLQLVLEGQVLALQQQMAENQAASWRKLKNSQEAQKRQATLVRKLQAKVLQYRSWCQDLEKRLEATGGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKLTVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	XM_006498796.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498796.1

ORF Insert Sequence:

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGACCTTCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG GGAGCTGCTT CAGAAGGCCA GAGGAGAGCT GCAGCAGCAA 
CTGGAAGTGC TGGAGCAGGA AGCGTGGCGA CTGCGGAGGA TGAACATGGA GCTGCAGCTG 
CAGGGGGACT CAGCTCAAGG GGAGAAGCTG GAGCAGCAGG AGGAGTTGCA CTTGGCTGTC 
CGGGAGAGGG AGCGCCTTCA GGAGACGCTG GTGGGCCTGG AAGCCAAGCA GTCAGAATCA 
CTAAGTGAGC TGCTCACTCT TCGAGAAGCC CTGGAGTCAA GTCGCCTAGA AGGGGAGTTA 
CTGAAGCAGG AGCGAGTGGA AGTGGCTGCA GCCTTGGCCA GGGCAGAACA GTCCATTGTG 
GAGCTGTCAG GTTCTGAGAA CAGCCTGAAG GCAGAGGTAG CTGATCTTCG AGCTGCAGCT 
GTCAAGCTTG GGGCCTTAAA CGAGGCTTTG GCTTTAGATA AAGTTGAACT GAACCAGCAG 
CTTCTCCAGT TGGAGCAGGA GAACCAGTCT CTATGCAGCA GAGTGGAGGC CGCAGAGCAG 
CTGAGAAGCG CTTTGCAGGT GGACCTGGCG GAGGCAGAAA GGCGCAGGGA AGCCCTGTGG 
GAGAAGAAAA CTCAGCTGGA GACTCAGCTG CAGAAGGCAG AGGAAGCGGG GGCTGAGCTA 
CAGGCAGAGC TGAGGGGCAC CCGAGAAGAA AAGGAAGAGC TTAAAGATAA ATTAAGTGAG 
GCACATCACC AGCAAGAAAC AGCTACAGCT CACCTAGAAC AGCTGCATCA GGACGCAGAA 
AGACAGGAAG AAACACTTGC TAGGGCAGTC CAGGAGAAGG AAGCCCTAGT ACGGGAGAGG 
GCAGCTCTTG AGGTGCGCCT GCAGGCTGTG GAGCGGGACC GACAGGACCT CACTGAACAT 
GTCCTGGGGC TCAGGTCAGC CAAGGAGCAA CTGGAGAGCA ATCTATTTGA GGCCCAACAG 
CAAAATTCTG TGATACAGGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATTATC 
CAAGCCAAGG AAGTAATTCA AGGGGAAGTG AAATGCCTGA AGCTGGAACT GGATGCTGAG 
AGGACCCGGG CAGAGCAGGA GTGGGATGCA GTAGCCAGGC AGCTAGCCCA AGCTGAGCAG 
GAGGGGCAAG CATCTCTGGA GCGACAGAAG GTGGCCCACG AGGAGGAGGT GAACAGGCTG 
CAAGAGAAAT GGGAGAAAGA GCGCTCCTGG CTGCAGCAGG AGCTGGATAA GACCCTGGAA 
ACCCTCGAGA GGGAGAGAGC AGAGCTGGAG ACAAAGCTGA GAGAGCAGCA GACAGAAATG 
GAAGCCATCC GTGCACAGAG GGAAGAGGAA CGAAGCCAGG CTGACAGCGC CCTGTATCAA 
ATGCAGCTAG AAACAGAGAA GGAGAGAGTA TCCCTCCTGG AGACACTGCT GAGGACCCAG 
AAGGAGCTGG CAGATGCTAG TCAACAGCTG GAAAGGCTGA GACAGGACAT GAAGATTCAG 
AAGTTAAAGG AACAGGAGAC CACTGGCATG CTGCAGGCCC AGCTTCAGGA AACACAGCAG 
GAGCTGAAGG AGGCAGCCCA GCAGCACAGG GACGACCTTG CTGCCTTCCA GAAGGACAAG 
CTGGACCTGC AGAAGCAGGT AGAGGACCTA ATGTCTCAGC TGGTAGCCCA CGATGACTCC 
CAGAGGCTGG TGAAAGAAGA GATTGAAGAG AAGGTGAAAG TGGCTCAAGA ATGCAGCCGG 
ATTCAGAAGG AACTGGAGAA AGAGAACGCC AGCCTGGCTC TGTCGCTGGT GGAAAAGGAA 
AAACGACTTC TCATTTTACA AGAAGCTGAC TCTGTAAGAC AGCAGGAGCT GAGCTCCTTG 
CGCCAGGACA TACAGGAGGC CCAGGAAGGG CAAAGAGAGC TGGGTGTACA GGTGGAACTA 
TTGAGGCAGG AGGTGAAGGA AAAGGAGGCT GACTTTGTGG CCCGGGAAGC ACAGCTGCTG 
GAGGAGTTGG AGGCCTCACG GGTTGCAGAG CAGCAACTTC GAGCGTCTTT GTGGGCCCAG 
GAAGCCAAGG CAACCCAGCT GCAGCTGCAG CTGCGCAGCA CTGAGAGCCA GCTAGAGGCA 
TTGGTTGCAG AACAACAGCC GGAGAACCAG GCACAGGCAC AGCTAGCTAG CCTCTGCTCT 
GTTCTGCAGC AGGCCCTGGG GTCTGCATGT GAGAGTAGGC CTGAGCTGAG GGGTGGAGGC 
GACTCTGCTC CTACCTTATG GGGCCCTGAT CCAGATCAGA ATGGAGCTTC CAGACTCTTT 
AAGAGATGGT CGCTCCCAAC GGCACTGTCA CCGGAGGCTG TGGCACTAGC TCTCCAGAAG 
CTCCATCAGG ACGTGTGGAA GGCTCGGCAG GCCCGGGATG ACCTAAGGGA CCAGGTCCAG 
AAGCTGGTGC AGCGCCTGAC TGACACGGAG GCCCAGAAGA GCCAGGTTCA CTCGGAGCTG 
CAGGACCTGC AGAGACAGCT CTCTCAGAGC CAGGAAGAGA AATCCAAGTG GGAAGGGAGG 
CAGAACTCCC TGGAGTCTGA GCTGAGGGAC TTGCACGAGA CCGCGGCATC CTTACAGAGT 
CGCCTCCGGC AGGCAGAACT GCAAAAAATG GAAGCTCAGA ATGATCGAGA GTTGCTTCAG 
GCATCCAAGG AGAAGCTGTC AGCCCAGGTT GAACATCTGC AAGCATGTGT TGCAGAAGCC 
CAGGCTCAGG CAGATGCAGC TGCTGTCCTG GAAGAAGATC TGCGAACTGC TCGCTCTGCA 
CTGAAACTGA AAAACGAGGA GCTAGAGAGT GAACGGGAGA GAGCCCAGGC TTTACAAGAA 
CAGGGTGAGC TGAAGGTGGC TCAGGGGAAA GCTTTGCAGG AGAACCTAGC CCTGCTGGCA 
CAGACCCTGT CTAACAGAGA GCGGGAGGTC GAGACGTTGC AGGCTGAGGT TCAGGAACTG 
GAGAAGCAGC GGGAGATGCA GAAGGCTGCT CTGGAGCTGC TCTCCCTCGA CCTGAAGAAG 
CGCAGTCGGG AGGTAGACCT GCAACAAGAA CAGATTCAGG AGCTGGAGCA GTGCAGGTCA 
GTCTTAGAGC ACTTGCCCAT GGCCGTCCAA GAGCGAGAGC AGAAACTGAG TGTGCAGCGG 
GACCAGATCA GGGAACTGGA GAACGATCGG GAGGCCCAGA GGAGTGTCCT GGAACACCAG 
CTTCTGGACC TGGAGCAGAA GGCTCAAGTG ATTGAGTCCC AGAGGGGACA GATTCAGGAT 
CTGAAAAAGC AACTGGGGAC ACTGGAATGC CTGGCCCTGG AACTGGAGGA AAGCCATCAC 
AAAGTGGAGA GCCAACAGAA GATGATCACA GAACTAGAGG GCCAGAGGGA AATGCAGAGG 
GTGGCTCTAA CCCACCTCAC ACTGGACCTG GAAGAAAGGA GCCAGGAGCT GCAGGCACAA 
AGCAGCCAGC TCCATGAGCT GGAGAACCAT AGCACCCATC TGGCAAAAGA GCTGCAGGAG 
AGGGACCAGG AGGTGACGTC CCAACGCCAG CAGATCGATG AGCTGCAGAA GCAGCAGGAA 
CAGCTGGCTC AGGCCCTGGA GAGGAAAGGC CAGGAGCTGG TGCTGCAGAA GGAAAGGATT 
CAGGTTCTGG AAGATCAGAG GACGCTGCAG ACCAAGATCT TAGAGGAGGA CCTAGAACAG 
ATCAAGCATT CCTTGAGAGA ACGCAGCCAG GAGCTGGCCT CTCAGTGGCA GCTGGTGCAT 
GAGCGTGCCG ACGATGGAAA GAGCCCGAGT AAAGGCCAGC GAGGGAGCCT GGAGCACTTG 
AAGCTGATCC TGCGTGATAA GGAGAAGGAG GTAGAGTGCC AGCAGGAGCG CATCCAGGAA 
CTTCAGGGCC ACATGGGCCA GCTGGAACAG CAGCTGCAGG GTCTGCACAG GAAGGTGGGG 
GAGACTAGCC TGCTCCTGAC CCATCGAGAG CAGGAGACAG CAACCCTGCA GCAGCATCTC 
CAGGAAGCCA AGGAACAAGG GGAACTGAGA GAGCAGGTAC TTCAGGGTCA GCTGGAGGAG 
GCCCAGAGAG ATCTGGCCCA GAGGGACCAT GAACTAGAGA CCCTCCGGCA GGAAAAACAG 
CAGACCCAGG ACCAGGAGGA AAGCATGAAG CTAAAGACAA GTGCTCTGCA GGCCGCTCTG 
GAGCAGGCCC ATGCGACACT GAAGGAGCGT CAGGGGGAGC TGGAAGAACA CAGGGAACAG 
GTGCGAAGGC TCCAGGAAGA ACTAGAAGTG GAGGGACGGC AGGTACGGGC CCTGGAGGAG 
GTGTTGGGGG ACCTAAGGGC TGAGTCTCGG GAGCACGAGA AGGCTGTGCT GGCCCTTCAA 
CAACGGTGTG CTGAGCAGGC CCAGGAACAT GAGGCAGAAG CCAGGACCCT GCAGGACAGC 
TGGCTGCAGG CCCAGGCCAC TCTCACAGAA CAAGAACAAG AGCTAGCGGC ACTGCGAGCG 
GAAAACCAGT ATTCCCGCCG ACAGGAAGAG GCTGCAGTTA GCCAGGCAGA GGCGCTGCAG 
GAGGCCCTCA GCAAAGCCCA GGCTGCCCTG CAGGAGAAAG AGCAGAGTCT GCTTGAACAG 
GCTGAGTTGA GCCACACCCT GGAGGCCAGC ACTGCCGCCT TGCAAGCTAC CCTGGACACC 
TGCCAGGCAA GTGCCCGGCA GCTGGAGGAG GCCCTGAGGA TTCGGGAAGG TGAGATCCAG 
GCCCAGGCTC TCCAACACCA CGAGGTCACG CAGCACCTCC AGCAGGAACT TTGCCAGAAG 
AAAGAAGAGC TGAGGCAGCT ACTAGAGAAG GCTGGGGCCC GGAGGAGTCA AGAAAATGGA 
ATCCAAGAGA AGCAAAGTCT GGAGCAAGAG AGACAAGAGG AAACAAGGAG GCTCCTTGAG 
AGCCTAAAGG AGCTACAGCT GACTGTAGCC CAAAGGGAAG AAGAGATTCT GATGCTGAGG 
GAGGCCTCGT CCCCACGCCA CAGAGCCCTC CCAGCCGAGA AGCCAGCTCT GCAACCGCTC 
CCAGCACAAC AGGAGTTAGA GCGGCTGCAG ACTGCCCTAA GGCAAACAGA GGCCAGGGAG 
ATTGAATGGA GGGAGAAGGC TCAGGACCTG GCTCTGTCCC TGGCCCAAAG CAAGGCCAGC 
ATCAGCAGTC TGCAGGAGAT AACCATGTTC CTACAAGCCT CTGTTCTAGA GCGGGAATCA 
GAACAACAAA GACTACAGGA GGAGTTGGTA CTCAGCAGAC AAGCTCTGGA AGAGCAGCAG 
TCAGGTGGCC CACACTCAAC CAGCAGAGCA GACCAGGGAC CAAAAGTGGG ACAGGGAAGC 
CAGTCTGGAG AGGTAGAGAC TGAGCCTAGT CCTGGAGTGG AGGAGAAGGA ACGGTTGACA 
CAGAGGCTTG AACGTCTGCA GCAAGCGGTG GCAGAGCTGG AAGTTGACCG AAGCAAGCTG 
CAGTGCCACA ATGCGCAGCT GCGGACCGCC TTGGAGCAGG TGGAACGAGA ACGGAGGAAG 
CTGAAGAGGG ATTCCGTGCG TGCATCCCGG GCAGGATCAC TGGAGGCCAG AGAAACTATG 
ACTTCATCCC CCACACAGCA GGATGGGAGA GGCAGTCAGA GGGGTTCCTC GGACAGCGTG 
CTTGTGGTTG AGCTTCAGAG AGAGGTAGCC CTGCTGCGAG CCCAGCTGGC CTTGGAGCGA 
AAGCAGAGGC AGGACTACAT TGCACGGTCC GTGCAGACCA GCCGTGAGCT AGCAGGCCTG 
CATCACAGCC TGTCTCACTC CCTCCTCACC GTGGCCCAGG CCCCTGAGGC CACTGTTCTG 
GAGGCTGAGA CCCGAAAGCT GGATGAGTCT CTGAATCAGA GTCTGACATC CCCAGGGCCA 
TGTTTGCTAC ATCCCAGTCT GGACACTACT CAAAATACCC ACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( XM_006498797.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498860.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant X2, mRNA.

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CloneID	OMu06037
Clone ID Related Accession (Same CDS sequence)	NM_001130000.1 , XM_006498796.1 , XM_006498797.3 , XM_006498797.4
Accession Version	XM_006498797.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498797.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGACCTTCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG GGAGCTGCTT CAGAAGGCCA GAGGAGAGCT GCAGCAGCAA 
CTGGAAGTGC TGGAGCAGGA AGCGTGGCGA CTGCGGAGGA TGAACATGGA GCTGCAGCTG 
CAGGGGGACT CAGCTCAAGG GGAGAAGCTG GAGCAGCAGG AGGAGTTGCA CTTGGCTGTC 
CGGGAGAGGG AGCGCCTTCA GGAGACGCTG GTGGGCCTGG AAGCCAAGCA GTCAGAATCA 
CTAAGTGAGC TGCTCACTCT TCGAGAAGCC CTGGAGTCAA GTCGCCTAGA AGGGGAGTTA 
CTGAAGCAGG AGCGAGTGGA AGTGGCTGCA GCCTTGGCCA GGGCAGAACA GTCCATTGTG 
GAGCTGTCAG GTTCTGAGAA CAGCCTGAAG GCAGAGGTAG CTGATCTTCG AGCTGCAGCT 
GTCAAGCTTG GGGCCTTAAA CGAGGCTTTG GCTTTAGATA AAGTTGAACT GAACCAGCAG 
CTTCTCCAGT TGGAGCAGGA GAACCAGTCT CTATGCAGCA GAGTGGAGGC CGCAGAGCAG 
CTGAGAAGCG CTTTGCAGGT GGACCTGGCG GAGGCAGAAA GGCGCAGGGA AGCCCTGTGG 
GAGAAGAAAA CTCAGCTGGA GACTCAGCTG CAGAAGGCAG AGGAAGCGGG GGCTGAGCTA 
CAGGCAGAGC TGAGGGGCAC CCGAGAAGAA AAGGAAGAGC TTAAAGATAA ATTAAGTGAG 
GCACATCACC AGCAAGAAAC AGCTACAGCT CACCTAGAAC AGCTGCATCA GGACGCAGAA 
AGACAGGAAG AAACACTTGC TAGGGCAGTC CAGGAGAAGG AAGCCCTAGT ACGGGAGAGG 
GCAGCTCTTG AGGTGCGCCT GCAGGCTGTG GAGCGGGACC GACAGGACCT CACTGAACAT 
GTCCTGGGGC TCAGGTCAGC CAAGGAGCAA CTGGAGAGCA ATCTATTTGA GGCCCAACAG 
CAAAATTCTG TGATACAGGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATTATC 
CAAGCCAAGG AAGTAATTCA AGGGGAAGTG AAATGCCTGA AGCTGGAACT GGATGCTGAG 
AGGACCCGGG CAGAGCAGGA GTGGGATGCA GTAGCCAGGC AGCTAGCCCA AGCTGAGCAG 
GAGGGGCAAG CATCTCTGGA GCGACAGAAG GTGGCCCACG AGGAGGAGGT GAACAGGCTG 
CAAGAGAAAT GGGAGAAAGA GCGCTCCTGG CTGCAGCAGG AGCTGGATAA GACCCTGGAA 
ACCCTCGAGA GGGAGAGAGC AGAGCTGGAG ACAAAGCTGA GAGAGCAGCA GACAGAAATG 
GAAGCCATCC GTGCACAGAG GGAAGAGGAA CGAAGCCAGG CTGACAGCGC CCTGTATCAA 
ATGCAGCTAG AAACAGAGAA GGAGAGAGTA TCCCTCCTGG AGACACTGCT GAGGACCCAG 
AAGGAGCTGG CAGATGCTAG TCAACAGCTG GAAAGGCTGA GACAGGACAT GAAGATTCAG 
AAGTTAAAGG AACAGGAGAC CACTGGCATG CTGCAGGCCC AGCTTCAGGA AACACAGCAG 
GAGCTGAAGG AGGCAGCCCA GCAGCACAGG GACGACCTTG CTGCCTTCCA GAAGGACAAG 
CTGGACCTGC AGAAGCAGGT AGAGGACCTA ATGTCTCAGC TGGTAGCCCA CGATGACTCC 
CAGAGGCTGG TGAAAGAAGA GATTGAAGAG AAGGTGAAAG TGGCTCAAGA ATGCAGCCGG 
ATTCAGAAGG AACTGGAGAA AGAGAACGCC AGCCTGGCTC TGTCGCTGGT GGAAAAGGAA 
AAACGACTTC TCATTTTACA AGAAGCTGAC TCTGTAAGAC AGCAGGAGCT GAGCTCCTTG 
CGCCAGGACA TACAGGAGGC CCAGGAAGGG CAAAGAGAGC TGGGTGTACA GGTGGAACTA 
TTGAGGCAGG AGGTGAAGGA AAAGGAGGCT GACTTTGTGG CCCGGGAAGC ACAGCTGCTG 
GAGGAGTTGG AGGCCTCACG GGTTGCAGAG CAGCAACTTC GAGCGTCTTT GTGGGCCCAG 
GAAGCCAAGG CAACCCAGCT GCAGCTGCAG CTGCGCAGCA CTGAGAGCCA GCTAGAGGCA 
TTGGTTGCAG AACAACAGCC GGAGAACCAG GCACAGGCAC AGCTAGCTAG CCTCTGCTCT 
GTTCTGCAGC AGGCCCTGGG GTCTGCATGT GAGAGTAGGC CTGAGCTGAG GGGTGGAGGC 
GACTCTGCTC CTACCTTATG GGGCCCTGAT CCAGATCAGA ATGGAGCTTC CAGACTCTTT 
AAGAGATGGT CGCTCCCAAC GGCACTGTCA CCGGAGGCTG TGGCACTAGC TCTCCAGAAG 
CTCCATCAGG ACGTGTGGAA GGCTCGGCAG GCCCGGGATG ACCTAAGGGA CCAGGTCCAG 
AAGCTGGTGC AGCGCCTGAC TGACACGGAG GCCCAGAAGA GCCAGGTTCA CTCGGAGCTG 
CAGGACCTGC AGAGACAGCT CTCTCAGAGC CAGGAAGAGA AATCCAAGTG GGAAGGGAGG 
CAGAACTCCC TGGAGTCTGA GCTGAGGGAC TTGCACGAGA CCGCGGCATC CTTACAGAGT 
CGCCTCCGGC AGGCAGAACT GCAAAAAATG GAAGCTCAGA ATGATCGAGA GTTGCTTCAG 
GCATCCAAGG AGAAGCTGTC AGCCCAGGTT GAACATCTGC AAGCATGTGT TGCAGAAGCC 
CAGGCTCAGG CAGATGCAGC TGCTGTCCTG GAAGAAGATC TGCGAACTGC TCGCTCTGCA 
CTGAAACTGA AAAACGAGGA GCTAGAGAGT GAACGGGAGA GAGCCCAGGC TTTACAAGAA 
CAGGGTGAGC TGAAGGTGGC TCAGGGGAAA GCTTTGCAGG AGAACCTAGC CCTGCTGGCA 
CAGACCCTGT CTAACAGAGA GCGGGAGGTC GAGACGTTGC AGGCTGAGGT TCAGGAACTG 
GAGAAGCAGC GGGAGATGCA GAAGGCTGCT CTGGAGCTGC TCTCCCTCGA CCTGAAGAAG 
CGCAGTCGGG AGGTAGACCT GCAACAAGAA CAGATTCAGG AGCTGGAGCA GTGCAGGTCA 
GTCTTAGAGC ACTTGCCCAT GGCCGTCCAA GAGCGAGAGC AGAAACTGAG TGTGCAGCGG 
GACCAGATCA GGGAACTGGA GAACGATCGG GAGGCCCAGA GGAGTGTCCT GGAACACCAG 
CTTCTGGACC TGGAGCAGAA GGCTCAAGTG ATTGAGTCCC AGAGGGGACA GATTCAGGAT 
CTGAAAAAGC AACTGGGGAC ACTGGAATGC CTGGCCCTGG AACTGGAGGA AAGCCATCAC 
AAAGTGGAGA GCCAACAGAA GATGATCACA GAACTAGAGG GCCAGAGGGA AATGCAGAGG 
GTGGCTCTAA CCCACCTCAC ACTGGACCTG GAAGAAAGGA GCCAGGAGCT GCAGGCACAA 
AGCAGCCAGC TCCATGAGCT GGAGAACCAT AGCACCCATC TGGCAAAAGA GCTGCAGGAG 
AGGGACCAGG AGGTGACGTC CCAACGCCAG CAGATCGATG AGCTGCAGAA GCAGCAGGAA 
CAGCTGGCTC AGGCCCTGGA GAGGAAAGGC CAGGAGCTGG TGCTGCAGAA GGAAAGGATT 
CAGGTTCTGG AAGATCAGAG GACGCTGCAG ACCAAGATCT TAGAGGAGGA CCTAGAACAG 
ATCAAGCATT CCTTGAGAGA ACGCAGCCAG GAGCTGGCCT CTCAGTGGCA GCTGGTGCAT 
GAGCGTGCCG ACGATGGAAA GAGCCCGAGT AAAGGCCAGC GAGGGAGCCT GGAGCACTTG 
AAGCTGATCC TGCGTGATAA GGAGAAGGAG GTAGAGTGCC AGCAGGAGCG CATCCAGGAA 
CTTCAGGGCC ACATGGGCCA GCTGGAACAG CAGCTGCAGG GTCTGCACAG GAAGGTGGGG 
GAGACTAGCC TGCTCCTGAC CCATCGAGAG CAGGAGACAG CAACCCTGCA GCAGCATCTC 
CAGGAAGCCA AGGAACAAGG GGAACTGAGA GAGCAGGTAC TTCAGGGTCA GCTGGAGGAG 
GCCCAGAGAG ATCTGGCCCA GAGGGACCAT GAACTAGAGA CCCTCCGGCA GGAAAAACAG 
CAGACCCAGG ACCAGGAGGA AAGCATGAAG CTAAAGACAA GTGCTCTGCA GGCCGCTCTG 
GAGCAGGCCC ATGCGACACT GAAGGAGCGT CAGGGGGAGC TGGAAGAACA CAGGGAACAG 
GTGCGAAGGC TCCAGGAAGA ACTAGAAGTG GAGGGACGGC AGGTACGGGC CCTGGAGGAG 
GTGTTGGGGG ACCTAAGGGC TGAGTCTCGG GAGCACGAGA AGGCTGTGCT GGCCCTTCAA 
CAACGGTGTG CTGAGCAGGC CCAGGAACAT GAGGCAGAAG CCAGGACCCT GCAGGACAGC 
TGGCTGCAGG CCCAGGCCAC TCTCACAGAA CAAGAACAAG AGCTAGCGGC ACTGCGAGCG 
GAAAACCAGT ATTCCCGCCG ACAGGAAGAG GCTGCAGTTA GCCAGGCAGA GGCGCTGCAG 
GAGGCCCTCA GCAAAGCCCA GGCTGCCCTG CAGGAGAAAG AGCAGAGTCT GCTTGAACAG 
GCTGAGTTGA GCCACACCCT GGAGGCCAGC ACTGCCGCCT TGCAAGCTAC CCTGGACACC 
TGCCAGGCAA GTGCCCGGCA GCTGGAGGAG GCCCTGAGGA TTCGGGAAGG TGAGATCCAG 
GCCCAGGCTC TCCAACACCA CGAGGTCACG CAGCACCTCC AGCAGGAACT TTGCCAGAAG 
AAAGAAGAGC TGAGGCAGCT ACTAGAGAAG GCTGGGGCCC GGAGGAGTCA AGAAAATGGA 
ATCCAAGAGA AGCAAAGTCT GGAGCAAGAG AGACAAGAGG AAACAAGGAG GCTCCTTGAG 
AGCCTAAAGG AGCTACAGCT GACTGTAGCC CAAAGGGAAG AAGAGATTCT GATGCTGAGG 
GAGGCCTCGT CCCCACGCCA CAGAGCCCTC CCAGCCGAGA AGCCAGCTCT GCAACCGCTC 
CCAGCACAAC AGGAGTTAGA GCGGCTGCAG ACTGCCCTAA GGCAAACAGA GGCCAGGGAG 
ATTGAATGGA GGGAGAAGGC TCAGGACCTG GCTCTGTCCC TGGCCCAAAG CAAGGCCAGC 
ATCAGCAGTC TGCAGGAGAT AACCATGTTC CTACAAGCCT CTGTTCTAGA GCGGGAATCA 
GAACAACAAA GACTACAGGA GGAGTTGGTA CTCAGCAGAC AAGCTCTGGA AGAGCAGCAG 
TCAGGTGGCC CACACTCAAC CAGCAGAGCA GACCAGGGAC CAAAAGTGGG ACAGGGAAGC 
CAGTCTGGAG AGGTAGAGAC TGAGCCTAGT CCTGGAGTGG AGGAGAAGGA ACGGTTGACA 
CAGAGGCTTG AACGTCTGCA GCAAGCGGTG GCAGAGCTGG AAGTTGACCG AAGCAAGCTG 
CAGTGCCACA ATGCGCAGCT GCGGACCGCC TTGGAGCAGG TGGAACGAGA ACGGAGGAAG 
CTGAAGAGGG ATTCCGTGCG TGCATCCCGG GCAGGATCAC TGGAGGCCAG AGAAACTATG 
ACTTCATCCC CCACACAGCA GGATGGGAGA GGCAGTCAGA GGGGTTCCTC GGACAGCGTG 
CTTGTGGTTG AGCTTCAGAG AGAGGTAGCC CTGCTGCGAG CCCAGCTGGC CTTGGAGCGA 
AAGCAGAGGC AGGACTACAT TGCACGGTCC GTGCAGACCA GCCGTGAGCT AGCAGGCCTG 
CATCACAGCC TGTCTCACTC CCTCCTCACC GTGGCCCAGG CCCCTGAGGC CACTGTTCTG 
GAGGCTGAGA CCCGAAAGCT GGATGAGTCT CTGAATCAGA GTCTGACATC CCCAGGGCCA 
TGTTTGCTAC ATCCCAGTCT GGACACTACT CAAAATACCC ACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498860.1
CDS	268..7575
Translation	METGSPGLNMKPQSLQLVLEGQVLALQQQMAENQAASWRKLKNSQEAQKRQATLVRKLQAKVLQYRSWCQDLEKRLEATGGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKLTVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	XM_006498797.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498797.3

ORF Insert Sequence:

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGACCTTCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG GGAGCTGCTT CAGAAGGCCA GAGGAGAGCT GCAGCAGCAA 
CTGGAAGTGC TGGAGCAGGA AGCGTGGCGA CTGCGGAGGA TGAACATGGA GCTGCAGCTG 
CAGGGGGACT CAGCTCAAGG GGAGAAGCTG GAGCAGCAGG AGGAGTTGCA CTTGGCTGTC 
CGGGAGAGGG AGCGCCTTCA GGAGACGCTG GTGGGCCTGG AAGCCAAGCA GTCAGAATCA 
CTAAGTGAGC TGCTCACTCT TCGAGAAGCC CTGGAGTCAA GTCGCCTAGA AGGGGAGTTA 
CTGAAGCAGG AGCGAGTGGA AGTGGCTGCA GCCTTGGCCA GGGCAGAACA GTCCATTGTG 
GAGCTGTCAG GTTCTGAGAA CAGCCTGAAG GCAGAGGTAG CTGATCTTCG AGCTGCAGCT 
GTCAAGCTTG GGGCCTTAAA CGAGGCTTTG GCTTTAGATA AAGTTGAACT GAACCAGCAG 
CTTCTCCAGT TGGAGCAGGA GAACCAGTCT CTATGCAGCA GAGTGGAGGC CGCAGAGCAG 
CTGAGAAGCG CTTTGCAGGT GGACCTGGCG GAGGCAGAAA GGCGCAGGGA AGCCCTGTGG 
GAGAAGAAAA CTCAGCTGGA GACTCAGCTG CAGAAGGCAG AGGAAGCGGG GGCTGAGCTA 
CAGGCAGAGC TGAGGGGCAC CCGAGAAGAA AAGGAAGAGC TTAAAGATAA ATTAAGTGAG 
GCACATCACC AGCAAGAAAC AGCTACAGCT CACCTAGAAC AGCTGCATCA GGACGCAGAA 
AGACAGGAAG AAACACTTGC TAGGGCAGTC CAGGAGAAGG AAGCCCTAGT ACGGGAGAGG 
GCAGCTCTTG AGGTGCGCCT GCAGGCTGTG GAGCGGGACC GACAGGACCT CACTGAACAT 
GTCCTGGGGC TCAGGTCAGC CAAGGAGCAA CTGGAGAGCA ATCTATTTGA GGCCCAACAG 
CAAAATTCTG TGATACAGGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATTATC 
CAAGCCAAGG AAGTAATTCA AGGGGAAGTG AAATGCCTGA AGCTGGAACT GGATGCTGAG 
AGGACCCGGG CAGAGCAGGA GTGGGATGCA GTAGCCAGGC AGCTAGCCCA AGCTGAGCAG 
GAGGGGCAAG CATCTCTGGA GCGACAGAAG GTGGCCCACG AGGAGGAGGT GAACAGGCTG 
CAAGAGAAAT GGGAGAAAGA GCGCTCCTGG CTGCAGCAGG AGCTGGATAA GACCCTGGAA 
ACCCTCGAGA GGGAGAGAGC AGAGCTGGAG ACAAAGCTGA GAGAGCAGCA GACAGAAATG 
GAAGCCATCC GTGCACAGAG GGAAGAGGAA CGAAGCCAGG CTGACAGCGC CCTGTATCAA 
ATGCAGCTAG AAACAGAGAA GGAGAGAGTA TCCCTCCTGG AGACACTGCT GAGGACCCAG 
AAGGAGCTGG CAGATGCTAG TCAACAGCTG GAAAGGCTGA GACAGGACAT GAAGATTCAG 
AAGTTAAAGG AACAGGAGAC CACTGGCATG CTGCAGGCCC AGCTTCAGGA AACACAGCAG 
GAGCTGAAGG AGGCAGCCCA GCAGCACAGG GACGACCTTG CTGCCTTCCA GAAGGACAAG 
CTGGACCTGC AGAAGCAGGT AGAGGACCTA ATGTCTCAGC TGGTAGCCCA CGATGACTCC 
CAGAGGCTGG TGAAAGAAGA GATTGAAGAG AAGGTGAAAG TGGCTCAAGA ATGCAGCCGG 
ATTCAGAAGG AACTGGAGAA AGAGAACGCC AGCCTGGCTC TGTCGCTGGT GGAAAAGGAA 
AAACGACTTC TCATTTTACA AGAAGCTGAC TCTGTAAGAC AGCAGGAGCT GAGCTCCTTG 
CGCCAGGACA TACAGGAGGC CCAGGAAGGG CAAAGAGAGC TGGGTGTACA GGTGGAACTA 
TTGAGGCAGG AGGTGAAGGA AAAGGAGGCT GACTTTGTGG CCCGGGAAGC ACAGCTGCTG 
GAGGAGTTGG AGGCCTCACG GGTTGCAGAG CAGCAACTTC GAGCGTCTTT GTGGGCCCAG 
GAAGCCAAGG CAACCCAGCT GCAGCTGCAG CTGCGCAGCA CTGAGAGCCA GCTAGAGGCA 
TTGGTTGCAG AACAACAGCC GGAGAACCAG GCACAGGCAC AGCTAGCTAG CCTCTGCTCT 
GTTCTGCAGC AGGCCCTGGG GTCTGCATGT GAGAGTAGGC CTGAGCTGAG GGGTGGAGGC 
GACTCTGCTC CTACCTTATG GGGCCCTGAT CCAGATCAGA ATGGAGCTTC CAGACTCTTT 
AAGAGATGGT CGCTCCCAAC GGCACTGTCA CCGGAGGCTG TGGCACTAGC TCTCCAGAAG 
CTCCATCAGG ACGTGTGGAA GGCTCGGCAG GCCCGGGATG ACCTAAGGGA CCAGGTCCAG 
AAGCTGGTGC AGCGCCTGAC TGACACGGAG GCCCAGAAGA GCCAGGTTCA CTCGGAGCTG 
CAGGACCTGC AGAGACAGCT CTCTCAGAGC CAGGAAGAGA AATCCAAGTG GGAAGGGAGG 
CAGAACTCCC TGGAGTCTGA GCTGAGGGAC TTGCACGAGA CCGCGGCATC CTTACAGAGT 
CGCCTCCGGC AGGCAGAACT GCAAAAAATG GAAGCTCAGA ATGATCGAGA GTTGCTTCAG 
GCATCCAAGG AGAAGCTGTC AGCCCAGGTT GAACATCTGC AAGCATGTGT TGCAGAAGCC 
CAGGCTCAGG CAGATGCAGC TGCTGTCCTG GAAGAAGATC TGCGAACTGC TCGCTCTGCA 
CTGAAACTGA AAAACGAGGA GCTAGAGAGT GAACGGGAGA GAGCCCAGGC TTTACAAGAA 
CAGGGTGAGC TGAAGGTGGC TCAGGGGAAA GCTTTGCAGG AGAACCTAGC CCTGCTGGCA 
CAGACCCTGT CTAACAGAGA GCGGGAGGTC GAGACGTTGC AGGCTGAGGT TCAGGAACTG 
GAGAAGCAGC GGGAGATGCA GAAGGCTGCT CTGGAGCTGC TCTCCCTCGA CCTGAAGAAG 
CGCAGTCGGG AGGTAGACCT GCAACAAGAA CAGATTCAGG AGCTGGAGCA GTGCAGGTCA 
GTCTTAGAGC ACTTGCCCAT GGCCGTCCAA GAGCGAGAGC AGAAACTGAG TGTGCAGCGG 
GACCAGATCA GGGAACTGGA GAACGATCGG GAGGCCCAGA GGAGTGTCCT GGAACACCAG 
CTTCTGGACC TGGAGCAGAA GGCTCAAGTG ATTGAGTCCC AGAGGGGACA GATTCAGGAT 
CTGAAAAAGC AACTGGGGAC ACTGGAATGC CTGGCCCTGG AACTGGAGGA AAGCCATCAC 
AAAGTGGAGA GCCAACAGAA GATGATCACA GAACTAGAGG GCCAGAGGGA AATGCAGAGG 
GTGGCTCTAA CCCACCTCAC ACTGGACCTG GAAGAAAGGA GCCAGGAGCT GCAGGCACAA 
AGCAGCCAGC TCCATGAGCT GGAGAACCAT AGCACCCATC TGGCAAAAGA GCTGCAGGAG 
AGGGACCAGG AGGTGACGTC CCAACGCCAG CAGATCGATG AGCTGCAGAA GCAGCAGGAA 
CAGCTGGCTC AGGCCCTGGA GAGGAAAGGC CAGGAGCTGG TGCTGCAGAA GGAAAGGATT 
CAGGTTCTGG AAGATCAGAG GACGCTGCAG ACCAAGATCT TAGAGGAGGA CCTAGAACAG 
ATCAAGCATT CCTTGAGAGA ACGCAGCCAG GAGCTGGCCT CTCAGTGGCA GCTGGTGCAT 
GAGCGTGCCG ACGATGGAAA GAGCCCGAGT AAAGGCCAGC GAGGGAGCCT GGAGCACTTG 
AAGCTGATCC TGCGTGATAA GGAGAAGGAG GTAGAGTGCC AGCAGGAGCG CATCCAGGAA 
CTTCAGGGCC ACATGGGCCA GCTGGAACAG CAGCTGCAGG GTCTGCACAG GAAGGTGGGG 
GAGACTAGCC TGCTCCTGAC CCATCGAGAG CAGGAGACAG CAACCCTGCA GCAGCATCTC 
CAGGAAGCCA AGGAACAAGG GGAACTGAGA GAGCAGGTAC TTCAGGGTCA GCTGGAGGAG 
GCCCAGAGAG ATCTGGCCCA GAGGGACCAT GAACTAGAGA CCCTCCGGCA GGAAAAACAG 
CAGACCCAGG ACCAGGAGGA AAGCATGAAG CTAAAGACAA GTGCTCTGCA GGCCGCTCTG 
GAGCAGGCCC ATGCGACACT GAAGGAGCGT CAGGGGGAGC TGGAAGAACA CAGGGAACAG 
GTGCGAAGGC TCCAGGAAGA ACTAGAAGTG GAGGGACGGC AGGTACGGGC CCTGGAGGAG 
GTGTTGGGGG ACCTAAGGGC TGAGTCTCGG GAGCACGAGA AGGCTGTGCT GGCCCTTCAA 
CAACGGTGTG CTGAGCAGGC CCAGGAACAT GAGGCAGAAG CCAGGACCCT GCAGGACAGC 
TGGCTGCAGG CCCAGGCCAC TCTCACAGAA CAAGAACAAG AGCTAGCGGC ACTGCGAGCG 
GAAAACCAGT ATTCCCGCCG ACAGGAAGAG GCTGCAGTTA GCCAGGCAGA GGCGCTGCAG 
GAGGCCCTCA GCAAAGCCCA GGCTGCCCTG CAGGAGAAAG AGCAGAGTCT GCTTGAACAG 
GCTGAGTTGA GCCACACCCT GGAGGCCAGC ACTGCCGCCT TGCAAGCTAC CCTGGACACC 
TGCCAGGCAA GTGCCCGGCA GCTGGAGGAG GCCCTGAGGA TTCGGGAAGG TGAGATCCAG 
GCCCAGGCTC TCCAACACCA CGAGGTCACG CAGCACCTCC AGCAGGAACT TTGCCAGAAG 
AAAGAAGAGC TGAGGCAGCT ACTAGAGAAG GCTGGGGCCC GGAGGAGTCA AGAAAATGGA 
ATCCAAGAGA AGCAAAGTCT GGAGCAAGAG AGACAAGAGG AAACAAGGAG GCTCCTTGAG 
AGCCTAAAGG AGCTACAGCT GACTGTAGCC CAAAGGGAAG AAGAGATTCT GATGCTGAGG 
GAGGCCTCGT CCCCACGCCA CAGAGCCCTC CCAGCCGAGA AGCCAGCTCT GCAACCGCTC 
CCAGCACAAC AGGAGTTAGA GCGGCTGCAG ACTGCCCTAA GGCAAACAGA GGCCAGGGAG 
ATTGAATGGA GGGAGAAGGC TCAGGACCTG GCTCTGTCCC TGGCCCAAAG CAAGGCCAGC 
ATCAGCAGTC TGCAGGAGAT AACCATGTTC CTACAAGCCT CTGTTCTAGA GCGGGAATCA 
GAACAACAAA GACTACAGGA GGAGTTGGTA CTCAGCAGAC AAGCTCTGGA AGAGCAGCAG 
TCAGGTGGCC CACACTCAAC CAGCAGAGCA GACCAGGGAC CAAAAGTGGG ACAGGGAAGC 
CAGTCTGGAG AGGTAGAGAC TGAGCCTAGT CCTGGAGTGG AGGAGAAGGA ACGGTTGACA 
CAGAGGCTTG AACGTCTGCA GCAAGCGGTG GCAGAGCTGG AAGTTGACCG AAGCAAGCTG 
CAGTGCCACA ATGCGCAGCT GCGGACCGCC TTGGAGCAGG TGGAACGAGA ACGGAGGAAG 
CTGAAGAGGG ATTCCGTGCG TGCATCCCGG GCAGGATCAC TGGAGGCCAG AGAAACTATG 
ACTTCATCCC CCACACAGCA GGATGGGAGA GGCAGTCAGA GGGGTTCCTC GGACAGCGTG 
CTTGTGGTTG AGCTTCAGAG AGAGGTAGCC CTGCTGCGAG CCCAGCTGGC CTTGGAGCGA 
AAGCAGAGGC AGGACTACAT TGCACGGTCC GTGCAGACCA GCCGTGAGCT AGCAGGCCTG 
CATCACAGCC TGTCTCACTC CCTCCTCACC GTGGCCCAGG CCCCTGAGGC CACTGTTCTG 
GAGGCTGAGA CCCGAAAGCT GGATGAGTCT CTGAATCAGA GTCTGACATC CCCAGGGCCA 
TGTTTGCTAC ATCCCAGTCT GGACACTACT CAAAATACCC ACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( XM_006498798.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498861.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant X3, mRNA.

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CloneID	OMu30042
Clone ID Related Accession (Same CDS sequence)	XM_006498798.1
Accession Version	XM_006498798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGACCTTCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG GGAGCTGCTT CAGAAGGCCA GAGGAGAGCT GCAGCAGCAA 
CTGGAAGTGC TGGAGCAGGA AGCGTGGCGA CTGCGGAGGA TGAACATGGA GCTGCAGCTG 
CAGGGGGACT CAGCTCAAGG GGAGAAGCTG GAGCAGCAGG AGGAGTTGCA CTTGGCTGTC 
CGGGAGAGGG AGCGCCTTCA GGAGACGCTG GTGGGCCTGG AAGCCAAGCA GTCAGAATCA 
CTAAGTGAGC TGCTCACTCT TCGAGAAGCC CTGGAGTCAA GTCGCCTAGA AGGGGAGTTA 
CTGAAGCAGG AGCGAGTGGA AGTGGCTGCA GCCTTGGCCA GGGCAGAACA GTCCATTGTG 
GAGCTGTCAG GTTCTGAGAA CAGCCTGAAG GCAGAGGTAG CTGATCTTCG AGCTGCAGCT 
GTCAAGCTTG GGGCCTTAAA CGAGGCTTTG GCTTTAGATA AAGTTGAACT GAACCAGCAG 
CTTCTCCAGT TGGAGCAGGA GAACCAGTCT CTATGCAGCA GAGTGGAGGC CGCAGAGCAG 
CTGAGAAGCG CTTTGCAGGT GGACCTGGCG GAGGCAGAAA GGCGCAGGGA AGCCCTGTGG 
GAGAAGAAAA CTCAGCTGGA GACTCAGCTG CAGAAGGCAG AGGAAGCGGG GGCTGAGCTA 
CAGGCAGAGC TGAGGGGCAC CCGAGAAGAA AAGGAAGAGC TTAAAGATAA ATTAAGTGAG 
GCACATCACC AGCAAGAAAC AGCTACAGCT CACCTAGAAC AGCTGCATCA GGACGCAGAA 
AGACAGGAAG AAACACTTGC TAGGGCAGTC CAGGAGAAGG AAGCCCTAGT ACGGGAGAGG 
GCAGCTCTTG AGGTGCGCCT GCAGGCTGTG GAGCGGGACC GACAGGACCT CACTGAACAT 
GTCCTGGGGC TCAGGTCAGC CAAGGAGCAA CTGGAGAGCA ATCTATTTGA GGCCCAACAG 
CAAAATTCTG TGATACAGGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATTATC 
CAAGCCAAGG AAGTAATTCA AGGGGAAGTG AAATGCCTGA AGCTGGAACT GGATGCTGAG 
AGGACCCGGG CAGAGCAGGA GTGGGATGCA GTAGCCAGGC AGCTAGCCCA AGCTGAGCAG 
GAGGGGCAAG CATCTCTGGA GCGACAGAAG GTGGCCCACG AGGAGGAGGT GAACAGGCTG 
CAAGAGAAAT GGGAGAAAGA GCGCTCCTGG CTGCAGCAGG AGCTGGATAA GACCCTGGAA 
ACCCTCGAGA GGGAGAGAGC AGAGCTGGAG ACAAAGCTGA GAGAGCAGCA GACAGAAATG 
GAAGCCATCC GTGCACAGAG GGAAGAGGAA CGAAGCCAGG CTGACAGCGC CCTGTATCAA 
ATGCAGCTAG AAACAGAGAA GGAGAGAGTA TCCCTCCTGG AGACACTGCT GAGGACCCAG 
AAGGAGCTGG CAGATGCTAG TCAACAGCTG GAAAGGCTGA GACAGGACAT GAAGATTCAG 
AAGTTAAAGG AACAGGAGAC CACTGGCATG CTGCAGGCCC AGCTTCAGGA AACACAGCAG 
GAGCTGAAGG AGGCAGCCCA GCAGCACAGG GACGACCTTG CTGCCTTCCA GAAGGACAAG 
CTGGACCTGC AGAAGCAGGT AGAGGACCTA ATGTCTCAGC TGGTAGCCCA CGATGACTCC 
CAGAGGCTGG TGAAAGAAGA GATTGAAGAG AAGGTGAAAG TGGCTCAAGA ATGCAGCCGG 
ATTCAGAAGG AACTGGAGAA AGAGAACGCC AGCCTGGCTC TGTCGCTGGT GGAAAAGGAA 
AAACGACTTC TCATTTTACA AGAAGCTGAC TCTGTAAGAC AGCAGGAGCT GAGCTCCTTG 
CGCCAGGACA TACAGGAGGC CCAGGAAGGG CAAAGAGAGC TGGGTGTACA GGTGGAACTA 
TTGAGGCAGG AGGTGAAGGA AAAGGAGGCT GACTTTGTGG CCCGGGAAGC ACAGCTGCTG 
GAGGAGTTGG AGGCCTCACG GGTTGCAGAG CAGCAACTTC GAGCGTCTTT GTGGGCCCAG 
GAAGCCAAGG CAACCCAGCT GCAGCTGCAG CTGCGCAGCA CTGAGAGCCA GCTAGAGGCA 
TTGGTTGCAG AACAACAGCC GGAGAACCAG GCACAGGCAC AGCTAGCTAG CCTCTGCTCT 
GTTCTGCAGC AGGCCCTGGG GTCTGCATGT GAGAGTAGGC CTGAGCTGAG GGGTGGAGGC 
GACTCTGCTC CTACCTTATG GGGCCCTGAT CCAGATCAGA ATGGAGCTTC CAGACTCTTT 
AAGAGATGGT CGCTCCCAAC GGCACTGTCA CCGGAGGCTG TGGCACTAGC TCTCCAGAAG 
CTCCATCAGG ACGTGTGGAA GGCTCGGCAG GCCCGGGATG ACCTAAGGGA CCAGGTCCAG 
AAGCTGGTGC AGCGCCTGAC TGACACGGAG GCCCAGAAGA GCCAGGTTCA CTCGGAGCTG 
CAGGACCTGC AGAGACAGCT CTCTCAGAGC CAGGAAGAGA AATCCAAGTG GGAAGGGAGG 
CAGAACTCCC TGGAGTCTGA GCTGAGGGAC TTGCACGAGA CCGCGGCATC CTTACAGAGT 
CGCCTCCGGC AGGCAGAACT GCAAAAAATG GAAGCTCAGA ATGATCGAGA GTTGCTTCAG 
GCATCCAAGG AGAAGCTGTC AGCCCAGGTT GAACATCTGC AAGCATGTGT TGCAGAAGCC 
CAGGCTCAGG CAGATGCAGC TGCTGTCCTG GAAGAAGATC TGCGAACTGC TCGCTCTGCA 
CTGAAACTGA AAAACGAGGA GCTAGAGAGT GAACGGGAGA GAGCCCAGGC TTTACAAGAA 
CAGGGTGAGC TGAAGGTGGC TCAGGGGAAA GCTTTGCAGG AGAACCTAGC CCTGCTGGCA 
CAGACCCTGT CTAACAGAGA GCGGGAGGTC GAGACGTTGC AGGCTGAGGT TCAGGAACTG 
GAGAAGCAGC GGGAGATGCA GAAGGCTGCT CTGGAGCTGC TCTCCCTCGA CCTGAAGAAG 
CGCAGTCGGG AGGTAGACCT GCAACAAGAA CAGATTCAGG AGCTGGAGCA GTGCAGGTCA 
GTCTTAGAGC ACTTGCCCAT GGCCGTCCAA GAGCGAGAGC AGAAACTGAG TGTGCAGCGG 
GACCAGATCA GGGAACTGGA GAACGATCGG GAGGCCCAGA GGAGTGTCCT GGAACACCAG 
CTTCTGGACC TGGAGCAGAA GGCTCAAGTG ATTGAGTCCC AGAGGGGACA GATTCAGGAT 
CTGAAAAAGC AACTGGGGAC ACTGGAATGC CTGGCCCTGG AACTGGAGGA AAGCCATCAC 
AAAGTGGAGA GCCAACAGAA GATGATCACA GAACTAGAGG GCCAGAGGGA AATGCAGAGG 
GTGGCTCTAA CCCACCTCAC ACTGGACCTG GAAGAAAGGA GCCAGGAGCT GCAGGCACAA 
AGCAGCCAGC TCCATGAGCT GGAGAACCAT AGCACCCATC TGGCAAAAGA GCTGCAGGAG 
AGGGACCAGG AGGTGACGTC CCAACGCCAG CAGATCGATG AGCTGCAGAA GCAGCAGGAA 
CAGCTGGCTC AGGCCCTGGA GAGGAAAGGC CAGGAGCTGG TGCTGCAGAA GGAAAGGATT 
CAGGTTCTGG AAGATCAGAG GACGCTGCAG ACCAAGATCT TAGAGGAGGA CCTAGAACAG 
ATCAAGCATT CCTTGAGAGA ACGCAGCCAG GAGCTGGCCT CTCAGTGGCA GCTGGTGCAT 
GAGCGTGCCG ACGATGGAAA GAGCCCGAGT AAAGGCCAGC GAGGGAGCCT GGAGCACTTG 
AAGCTGATCC TGCGTGATAA GGAGAAGGAG GTAGAGTGCC AGCAGGAGCG CATCCAGGAA 
CTTCAGGGCC ACATGGGCCA GCTGGAACAG CAGCTGCAGG GTCTGCACAG GAAGGTGGGG 
GAGACTAGCC TGCTCCTGAC CCATCGAGAG CAGGAGACAG CAACCCTGCA GCAGCATCTC 
CAGGAAGCCA AGGAACAAGG GGAACTGAGA GAGCAGGTAC TTCAGGGTCA GCTGGAGGAG 
GCCCAGAGAG ATCTGGCCCA GAGGGACCAT GAACTAGAGA CCCTCCGGCA GGAAAAACAG 
CAGACCCAGG ACCAGGAGGA AAGCATGAAG CTAAAGACAA GTGCTCTGCA GGCCGCTCTG 
GAGCAGGCCC ATGCGACACT GAAGGAGCGT CAGGGGGAGC TGGAAGAACA CAGGGAACAG 
GTGCGAAGGC TCCAGGAAGA ACTAGAAGTG GAGGGACGGC AGGTACGGGC CCTGGAGGAG 
GTGTTGGGGG ACCTAAGGGC TGAGTCTCGG GAGCACGAGA AGGCTGTGCT GGCCCTTCAA 
CAACGGTGTG CTGAGCAGGC CCAGGAACAT GAGGCAGAAG CCAGGACCCT GCAGGACAGC 
TGGCTGCAGG CCCAGGCCAC TCTCACAGAA CAAGAACAAG AGCTAGCGGC ACTGCGAGCG 
GAAAACCAGT ATTCCCGCCG ACAGGAAGAG GCTGCAGTTA GCCAGGCAGA GGCGCTGCAG 
GAGGCCCTCA GCAAAGCCCA GGCTGCCCTG CAGGAGAAAG AGCAGAGTCT GCTTGAACAG 
GCTGAGTTGA GCCACACCCT GGAGGCCAGC ACTGCCGCCT TGCAAGCTAC CCTGGACACC 
TGCCAGGCAA GTGCCCGGCA GCTGGAGGAG GCCCTGAGGA TTCGGGAAGG TGAGATCCAG 
GCCCAGGCTC TCCAACACCA CGAGGTCACG CAGCACCTCC AGCAGGAACT TTGCCAGAAG 
AAAGAAGAGC TGAGGCAGCT ACTAGAGAAG GCTGGGGCCC GGAGGAGTCA AGAAAATGGA 
ATCCAAGAGA AGCAAAGTCT GGAGCAAGAG AGACAAGAGG AAACAAGGAG GCTCCTTGAG 
AGCCTAAAGG AGCTACAGCT GACTGTAGCC CAAAGGGAAG AAGAGATTCT GATGCTGAGG 
GAGGCCTCGT CCCCACGCCA CAGAGCCCTC CCAGCCGAGA AGCCAGCTCT GCAACCGCTC 
CCAGCACAAC AGGAGTTAGA GCGGCTGCAG ACTGCCCTAA GGCAAACAGA GGCCAGGGAG 
ATTGAATGGA GGGAGAAGGC TCAGGACCTG GCTCTGTCCC TGGCCCAAAG CAAGGCCAGC 
ATCAGCAGTC TGCAGGAGAT AACCATGTTC CTACAAGCCT CTGTTCTAGA GCGGGAATCA 
GAACAACAAA GACTACAGGT AGAGACTGAG CCTAGTCCTG GAGTGGAGGA GAAGGAACGG 
TTGACACAGA GGCTTGAACG TCTGCAGCAA GCGGTGGCAG AGCTGGAAGT TGACCGAAGC 
AAGCTGCAGT GCCACAATGC GCAGCTGCGG ACCGCCTTGG AGCAGGTGGA ACGAGAACGG 
AGGAAGCTGA AGAGGGATTC CGTGCGTGCA TCCCGGGCAG GATCACTGGA GGCCAGAGAA 
ACTATGACTT CATCCCCCAC ACAGCAGGAT GGGAGAGGCA GTCAGAGGGG TTCCTCGGAC 
AGCGTGCTTG TGGTTGAGCT TCAGAGAGAG GTAGCCCTGC TGCGAGCCCA GCTGGCCTTG 
GAGCGAAAGC AGAGGCAGGA CTACATTGCA CGGTCCGTGC AGACCAGCCG TGAGCTAGCA 
GGCCTGCATC ACAGCCTGTC TCACTCCCTC CTCACCGTGG CCCAGGCCCC TGAGGCCACT 
GTTCTGGAGG CTGAGACCCG AAAGCTGGAT GAGTCTCTGA ATCAGAGTCT GACATCCCCA 
GGGCCATGTT TGCTACATCC CAGTCTGGAC ACTACTCAAA ATACCCACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498861.1
CDS	301..7494
Translation	METGSPGLNMKPQSLQLVLEGQVLALQQQMAENQAASWRKLKNSQEAQKRQATLVRKLQAKVLQYRSWCQDLEKRLEATGGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKLTVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	XM_006498798.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498798.1

ORF Insert Sequence:

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGACCTTCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG GGAGCTGCTT CAGAAGGCCA GAGGAGAGCT GCAGCAGCAA 
CTGGAAGTGC TGGAGCAGGA AGCGTGGCGA CTGCGGAGGA TGAACATGGA GCTGCAGCTG 
CAGGGGGACT CAGCTCAAGG GGAGAAGCTG GAGCAGCAGG AGGAGTTGCA CTTGGCTGTC 
CGGGAGAGGG AGCGCCTTCA GGAGACGCTG GTGGGCCTGG AAGCCAAGCA GTCAGAATCA 
CTAAGTGAGC TGCTCACTCT TCGAGAAGCC CTGGAGTCAA GTCGCCTAGA AGGGGAGTTA 
CTGAAGCAGG AGCGAGTGGA AGTGGCTGCA GCCTTGGCCA GGGCAGAACA GTCCATTGTG 
GAGCTGTCAG GTTCTGAGAA CAGCCTGAAG GCAGAGGTAG CTGATCTTCG AGCTGCAGCT 
GTCAAGCTTG GGGCCTTAAA CGAGGCTTTG GCTTTAGATA AAGTTGAACT GAACCAGCAG 
CTTCTCCAGT TGGAGCAGGA GAACCAGTCT CTATGCAGCA GAGTGGAGGC CGCAGAGCAG 
CTGAGAAGCG CTTTGCAGGT GGACCTGGCG GAGGCAGAAA GGCGCAGGGA AGCCCTGTGG 
GAGAAGAAAA CTCAGCTGGA GACTCAGCTG CAGAAGGCAG AGGAAGCGGG GGCTGAGCTA 
CAGGCAGAGC TGAGGGGCAC CCGAGAAGAA AAGGAAGAGC TTAAAGATAA ATTAAGTGAG 
GCACATCACC AGCAAGAAAC AGCTACAGCT CACCTAGAAC AGCTGCATCA GGACGCAGAA 
AGACAGGAAG AAACACTTGC TAGGGCAGTC CAGGAGAAGG AAGCCCTAGT ACGGGAGAGG 
GCAGCTCTTG AGGTGCGCCT GCAGGCTGTG GAGCGGGACC GACAGGACCT CACTGAACAT 
GTCCTGGGGC TCAGGTCAGC CAAGGAGCAA CTGGAGAGCA ATCTATTTGA GGCCCAACAG 
CAAAATTCTG TGATACAGGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATTATC 
CAAGCCAAGG AAGTAATTCA AGGGGAAGTG AAATGCCTGA AGCTGGAACT GGATGCTGAG 
AGGACCCGGG CAGAGCAGGA GTGGGATGCA GTAGCCAGGC AGCTAGCCCA AGCTGAGCAG 
GAGGGGCAAG CATCTCTGGA GCGACAGAAG GTGGCCCACG AGGAGGAGGT GAACAGGCTG 
CAAGAGAAAT GGGAGAAAGA GCGCTCCTGG CTGCAGCAGG AGCTGGATAA GACCCTGGAA 
ACCCTCGAGA GGGAGAGAGC AGAGCTGGAG ACAAAGCTGA GAGAGCAGCA GACAGAAATG 
GAAGCCATCC GTGCACAGAG GGAAGAGGAA CGAAGCCAGG CTGACAGCGC CCTGTATCAA 
ATGCAGCTAG AAACAGAGAA GGAGAGAGTA TCCCTCCTGG AGACACTGCT GAGGACCCAG 
AAGGAGCTGG CAGATGCTAG TCAACAGCTG GAAAGGCTGA GACAGGACAT GAAGATTCAG 
AAGTTAAAGG AACAGGAGAC CACTGGCATG CTGCAGGCCC AGCTTCAGGA AACACAGCAG 
GAGCTGAAGG AGGCAGCCCA GCAGCACAGG GACGACCTTG CTGCCTTCCA GAAGGACAAG 
CTGGACCTGC AGAAGCAGGT AGAGGACCTA ATGTCTCAGC TGGTAGCCCA CGATGACTCC 
CAGAGGCTGG TGAAAGAAGA GATTGAAGAG AAGGTGAAAG TGGCTCAAGA ATGCAGCCGG 
ATTCAGAAGG AACTGGAGAA AGAGAACGCC AGCCTGGCTC TGTCGCTGGT GGAAAAGGAA 
AAACGACTTC TCATTTTACA AGAAGCTGAC TCTGTAAGAC AGCAGGAGCT GAGCTCCTTG 
CGCCAGGACA TACAGGAGGC CCAGGAAGGG CAAAGAGAGC TGGGTGTACA GGTGGAACTA 
TTGAGGCAGG AGGTGAAGGA AAAGGAGGCT GACTTTGTGG CCCGGGAAGC ACAGCTGCTG 
GAGGAGTTGG AGGCCTCACG GGTTGCAGAG CAGCAACTTC GAGCGTCTTT GTGGGCCCAG 
GAAGCCAAGG CAACCCAGCT GCAGCTGCAG CTGCGCAGCA CTGAGAGCCA GCTAGAGGCA 
TTGGTTGCAG AACAACAGCC GGAGAACCAG GCACAGGCAC AGCTAGCTAG CCTCTGCTCT 
GTTCTGCAGC AGGCCCTGGG GTCTGCATGT GAGAGTAGGC CTGAGCTGAG GGGTGGAGGC 
GACTCTGCTC CTACCTTATG GGGCCCTGAT CCAGATCAGA ATGGAGCTTC CAGACTCTTT 
AAGAGATGGT CGCTCCCAAC GGCACTGTCA CCGGAGGCTG TGGCACTAGC TCTCCAGAAG 
CTCCATCAGG ACGTGTGGAA GGCTCGGCAG GCCCGGGATG ACCTAAGGGA CCAGGTCCAG 
AAGCTGGTGC AGCGCCTGAC TGACACGGAG GCCCAGAAGA GCCAGGTTCA CTCGGAGCTG 
CAGGACCTGC AGAGACAGCT CTCTCAGAGC CAGGAAGAGA AATCCAAGTG GGAAGGGAGG 
CAGAACTCCC TGGAGTCTGA GCTGAGGGAC TTGCACGAGA CCGCGGCATC CTTACAGAGT 
CGCCTCCGGC AGGCAGAACT GCAAAAAATG GAAGCTCAGA ATGATCGAGA GTTGCTTCAG 
GCATCCAAGG AGAAGCTGTC AGCCCAGGTT GAACATCTGC AAGCATGTGT TGCAGAAGCC 
CAGGCTCAGG CAGATGCAGC TGCTGTCCTG GAAGAAGATC TGCGAACTGC TCGCTCTGCA 
CTGAAACTGA AAAACGAGGA GCTAGAGAGT GAACGGGAGA GAGCCCAGGC TTTACAAGAA 
CAGGGTGAGC TGAAGGTGGC TCAGGGGAAA GCTTTGCAGG AGAACCTAGC CCTGCTGGCA 
CAGACCCTGT CTAACAGAGA GCGGGAGGTC GAGACGTTGC AGGCTGAGGT TCAGGAACTG 
GAGAAGCAGC GGGAGATGCA GAAGGCTGCT CTGGAGCTGC TCTCCCTCGA CCTGAAGAAG 
CGCAGTCGGG AGGTAGACCT GCAACAAGAA CAGATTCAGG AGCTGGAGCA GTGCAGGTCA 
GTCTTAGAGC ACTTGCCCAT GGCCGTCCAA GAGCGAGAGC AGAAACTGAG TGTGCAGCGG 
GACCAGATCA GGGAACTGGA GAACGATCGG GAGGCCCAGA GGAGTGTCCT GGAACACCAG 
CTTCTGGACC TGGAGCAGAA GGCTCAAGTG ATTGAGTCCC AGAGGGGACA GATTCAGGAT 
CTGAAAAAGC AACTGGGGAC ACTGGAATGC CTGGCCCTGG AACTGGAGGA AAGCCATCAC 
AAAGTGGAGA GCCAACAGAA GATGATCACA GAACTAGAGG GCCAGAGGGA AATGCAGAGG 
GTGGCTCTAA CCCACCTCAC ACTGGACCTG GAAGAAAGGA GCCAGGAGCT GCAGGCACAA 
AGCAGCCAGC TCCATGAGCT GGAGAACCAT AGCACCCATC TGGCAAAAGA GCTGCAGGAG 
AGGGACCAGG AGGTGACGTC CCAACGCCAG CAGATCGATG AGCTGCAGAA GCAGCAGGAA 
CAGCTGGCTC AGGCCCTGGA GAGGAAAGGC CAGGAGCTGG TGCTGCAGAA GGAAAGGATT 
CAGGTTCTGG AAGATCAGAG GACGCTGCAG ACCAAGATCT TAGAGGAGGA CCTAGAACAG 
ATCAAGCATT CCTTGAGAGA ACGCAGCCAG GAGCTGGCCT CTCAGTGGCA GCTGGTGCAT 
GAGCGTGCCG ACGATGGAAA GAGCCCGAGT AAAGGCCAGC GAGGGAGCCT GGAGCACTTG 
AAGCTGATCC TGCGTGATAA GGAGAAGGAG GTAGAGTGCC AGCAGGAGCG CATCCAGGAA 
CTTCAGGGCC ACATGGGCCA GCTGGAACAG CAGCTGCAGG GTCTGCACAG GAAGGTGGGG 
GAGACTAGCC TGCTCCTGAC CCATCGAGAG CAGGAGACAG CAACCCTGCA GCAGCATCTC 
CAGGAAGCCA AGGAACAAGG GGAACTGAGA GAGCAGGTAC TTCAGGGTCA GCTGGAGGAG 
GCCCAGAGAG ATCTGGCCCA GAGGGACCAT GAACTAGAGA CCCTCCGGCA GGAAAAACAG 
CAGACCCAGG ACCAGGAGGA AAGCATGAAG CTAAAGACAA GTGCTCTGCA GGCCGCTCTG 
GAGCAGGCCC ATGCGACACT GAAGGAGCGT CAGGGGGAGC TGGAAGAACA CAGGGAACAG 
GTGCGAAGGC TCCAGGAAGA ACTAGAAGTG GAGGGACGGC AGGTACGGGC CCTGGAGGAG 
GTGTTGGGGG ACCTAAGGGC TGAGTCTCGG GAGCACGAGA AGGCTGTGCT GGCCCTTCAA 
CAACGGTGTG CTGAGCAGGC CCAGGAACAT GAGGCAGAAG CCAGGACCCT GCAGGACAGC 
TGGCTGCAGG CCCAGGCCAC TCTCACAGAA CAAGAACAAG AGCTAGCGGC ACTGCGAGCG 
GAAAACCAGT ATTCCCGCCG ACAGGAAGAG GCTGCAGTTA GCCAGGCAGA GGCGCTGCAG 
GAGGCCCTCA GCAAAGCCCA GGCTGCCCTG CAGGAGAAAG AGCAGAGTCT GCTTGAACAG 
GCTGAGTTGA GCCACACCCT GGAGGCCAGC ACTGCCGCCT TGCAAGCTAC CCTGGACACC 
TGCCAGGCAA GTGCCCGGCA GCTGGAGGAG GCCCTGAGGA TTCGGGAAGG TGAGATCCAG 
GCCCAGGCTC TCCAACACCA CGAGGTCACG CAGCACCTCC AGCAGGAACT TTGCCAGAAG 
AAAGAAGAGC TGAGGCAGCT ACTAGAGAAG GCTGGGGCCC GGAGGAGTCA AGAAAATGGA 
ATCCAAGAGA AGCAAAGTCT GGAGCAAGAG AGACAAGAGG AAACAAGGAG GCTCCTTGAG 
AGCCTAAAGG AGCTACAGCT GACTGTAGCC CAAAGGGAAG AAGAGATTCT GATGCTGAGG 
GAGGCCTCGT CCCCACGCCA CAGAGCCCTC CCAGCCGAGA AGCCAGCTCT GCAACCGCTC 
CCAGCACAAC AGGAGTTAGA GCGGCTGCAG ACTGCCCTAA GGCAAACAGA GGCCAGGGAG 
ATTGAATGGA GGGAGAAGGC TCAGGACCTG GCTCTGTCCC TGGCCCAAAG CAAGGCCAGC 
ATCAGCAGTC TGCAGGAGAT AACCATGTTC CTACAAGCCT CTGTTCTAGA GCGGGAATCA 
GAACAACAAA GACTACAGGT AGAGACTGAG CCTAGTCCTG GAGTGGAGGA GAAGGAACGG 
TTGACACAGA GGCTTGAACG TCTGCAGCAA GCGGTGGCAG AGCTGGAAGT TGACCGAAGC 
AAGCTGCAGT GCCACAATGC GCAGCTGCGG ACCGCCTTGG AGCAGGTGGA ACGAGAACGG 
AGGAAGCTGA AGAGGGATTC CGTGCGTGCA TCCCGGGCAG GATCACTGGA GGCCAGAGAA 
ACTATGACTT CATCCCCCAC ACAGCAGGAT GGGAGAGGCA GTCAGAGGGG TTCCTCGGAC 
AGCGTGCTTG TGGTTGAGCT TCAGAGAGAG GTAGCCCTGC TGCGAGCCCA GCTGGCCTTG 
GAGCGAAAGC AGAGGCAGGA CTACATTGCA CGGTCCGTGC AGACCAGCCG TGAGCTAGCA 
GGCCTGCATC ACAGCCTGTC TCACTCCCTC CTCACCGTGG CCCAGGCCCC TGAGGCCACT 
GTTCTGGAGG CTGAGACCCG AAAGCTGGAT GAGTCTCTGA ATCAGAGTCT GACATCCCCA 
GGGCCATGTT TGCTACATCC CAGTCTGGAC ACTACTCAAA ATACCCACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( XM_006498799.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498862.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant X4, mRNA.

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CloneID	OMu30043
Clone ID Related Accession (Same CDS sequence)	XM_006498799.2 , XM_006498799.3
Accession Version	XM_006498799.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498799.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTCC GTGGATGCAG TATGTGGATG GAATTAGAGA GAAAATTCAT GTTCATCCAG 
CAGGGGCTGA TCCCTCAGCG CTGGGAGAGT GTAGAAGAGC CCAACCTGGA ACAGTTGCTG 
ATCCGATTGG AGGAGGAGCA GCAGAGGTGT GAGAGTCTAG TGGAGGTGAA CACCGAGCTT 
CGGCTGCACA TGGAGAAAGC CGATGTGGTG AATAAAGCCT TGCAGGAGGA CGTGGAAAAA 
CTGACAGTGG ACTGGAGTCG GGCCCGAGAT GAGTTAGTGA GGAAGGAGAG CCAGTGGCGG 
ATGGAGCAGG AGTTCTTCAA GGGCTATTTG AGAGGTGAAC ACGGTCGTCT TCTCAATCTA 
TGGCGAGAGG TGGTAACCTT CCGACGTCAC TTCCTGAAAA TGAAGTCAGC TACTGACAGA 
GACCTGACAG AGCTAAAGGC TGAACACGCA AGGCTTTCAG GGTCCCTGCT GACCTGCTGT 
CTGCGCCTGA CCCTGAGAGC ACAGTCTCGG GAGTCCAGTG GATCTGGGAG AACAGAGGAG 
AGTGAGCCAG CCCGGCTGCT GCTGCTCGTC GCTAAGACCC AGGCGCTGGA GAAGGAAGCC 
CATGAGAAGA GCCAGGAGTT AATGCAGCTC AAGAGTCACG GGGATCTAGA GAAGGCAGAG 
CTGCAGGACC GAGTGACGGA ACTCTCTGCT CTGCTAACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAGA AGATGGTAAA GGCTCTAAGA GAAACAATGG AGATCCTGGA GACAAACCAT 
GCAGAGTTAA TGGAGCATGA GGCATCTCTC AGTAGGAATG CTCAAGAGGA AAAGTTGTCT 
CTGCAGCAGG TGATCAAGGC TATAACCCAG GCACTGGCCT CAGTGGAAGA AGAAGACACT 
GTGACCCAGT CCTCAGGCCA TGAGGACTCG CTGCAGTCAG ACTGTAATGG CCTTTCCCAG 
TTTGATCCCC AGGACCCAGA CAGGGCTCTT ACCCTGGTGC AGTCAGTGCT GACTCGGAGA 
CAGCAGGCTG TGCAGGACCT CAGGCAGCAG CTTTCAGGCT GCCAGGAGGC TATGAGCTTC 
TTGCAGCAGC AGCACGATCA GTGGGAGGAA GAGGGCAGGG CCCTGAGGGA AAAGCTACAG 
AAGCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGGCCTTCA GGGAGAGGTG 
GACTCTCTCA CTGGGGAGCG GGATGCTCTG GCAGGCCAGA CTGTGGACCT TCAGGGAGAG 
GTGGACTCTC TCAGCAGGGA GCGGGAGCTG CTTCAGAAGG CCAGAGGAGA GCTGCAGCAG 
CAACTGGAAG TGCTGGAGCA GGAAGCGTGG CGACTGCGGA GGATGAACAT GGAGCTGCAG 
CTGCAGGGGG ACTCAGCTCA AGGGGAGAAG CTGGAGCAGC AGGAGGAGTT GCACTTGGCT 
GTCCGGGAGA GGGAGCGCCT TCAGGAGACG CTGGTGGGCC TGGAAGCCAA GCAGTCAGAA 
TCACTAAGTG AGCTGCTCAC TCTTCGAGAA GCCCTGGAGT CAAGTCGCCT AGAAGGGGAG 
TTACTGAAGC AGGAGCGAGT GGAAGTGGCT GCAGCCTTGG CCAGGGCAGA ACAGTCCATT 
GTGGAGCTGT CAGGTTCTGA GAACAGCCTG AAGGCAGAGG TAGCTGATCT TCGAGCTGCA 
GCTGTCAAGC TTGGGGCCTT AAACGAGGCT TTGGCTTTAG ATAAAGTTGA ACTGAACCAG 
CAGCTTCTCC AGTTGGAGCA GGAGAACCAG TCTCTATGCA GCAGAGTGGA GGCCGCAGAG 
CAGCTGAGAA GCGCTTTGCA GGTGGACCTG GCGGAGGCAG AAAGGCGCAG GGAAGCCCTG 
TGGGAGAAGA AAACTCAGCT GGAGACTCAG CTGCAGAAGG CAGAGGAAGC GGGGGCTGAG 
CTACAGGCAG AGCTGAGGGG CACCCGAGAA GAAAAGGAAG AGCTTAAAGA TAAATTAAGT 
GAGGCACATC ACCAGCAAGA AACAGCTACA GCTCACCTAG AACAGCTGCA TCAGGACGCA 
GAAAGACAGG AAGAAACACT TGCTAGGGCA GTCCAGGAGA AGGAAGCCCT AGTACGGGAG 
AGGGCAGCTC TTGAGGTGCG CCTGCAGGCT GTGGAGCGGG ACCGACAGGA CCTCACTGAA 
CATGTCCTGG GGCTCAGGTC AGCCAAGGAG CAACTGGAGA GCAATCTATT TGAGGCCCAA 
CAGCAAAATT CTGTGATACA GGTCACCAAG GGGCAGCTGG AGGTCCAGAT TCAAACTATT 
ATCCAAGCCA AGGAAGTAAT TCAAGGGGAA GTGAAATGCC TGAAGCTGGA ACTGGATGCT 
GAGAGGACCC GGGCAGAGCA GGAGTGGGAT GCAGTAGCCA GGCAGCTAGC CCAAGCTGAG 
CAGGAGGGGC AAGCATCTCT GGAGCGACAG AAGGTGGCCC ACGAGGAGGA GGTGAACAGG 
CTGCAAGAGA AATGGGAGAA AGAGCGCTCC TGGCTGCAGC AGGAGCTGGA TAAGACCCTG 
GAAACCCTCG AGAGGGAGAG AGCAGAGCTG GAGACAAAGC TGAGAGAGCA GCAGACAGAA 
ATGGAAGCCA TCCGTGCACA GAGGGAAGAG GAACGAAGCC AGGCTGACAG CGCCCTGTAT 
CAAATGCAGC TAGAAACAGA GAAGGAGAGA GTATCCCTCC TGGAGACACT GCTGAGGACC 
CAGAAGGAGC TGGCAGATGC TAGTCAACAG CTGGAAAGGC TGAGACAGGA CATGAAGATT 
CAGAAGTTAA AGGAACAGGA GACCACTGGC ATGCTGCAGG CCCAGCTTCA GGAAACACAG 
CAGGAGCTGA AGGAGGCAGC CCAGCAGCAC AGGGACGACC TTGCTGCCTT CCAGAAGGAC 
AAGCTGGACC TGCAGAAGCA GGTAGAGGAC CTAATGTCTC AGCTGGTAGC CCACGATGAC 
TCCCAGAGGC TGGTGAAAGA AGAGATTGAA GAGAAGGTGA AAGTGGCTCA AGAATGCAGC 
CGGATTCAGA AGGAACTGGA GAAAGAGAAC GCCAGCCTGG CTCTGTCGCT GGTGGAAAAG 
GAAAAACGAC TTCTCATTTT ACAAGAAGCT GACTCTGTAA GACAGCAGGA GCTGAGCTCC 
TTGCGCCAGG ACATACAGGA GGCCCAGGAA GGGCAAAGAG AGCTGGGTGT ACAGGTGGAA 
CTATTGAGGC AGGAGGTGAA GGAAAAGGAG GCTGACTTTG TGGCCCGGGA AGCACAGCTG 
CTGGAGGAGT TGGAGGCCTC ACGGGTTGCA GAGCAGCAAC TTCGAGCGTC TTTGTGGGCC 
CAGGAAGCCA AGGCAACCCA GCTGCAGCTG CAGCTGCGCA GCACTGAGAG CCAGCTAGAG 
GCATTGGTTG CAGAACAACA GCCGGAGAAC CAGGCACAGG CACAGCTAGC TAGCCTCTGC 
TCTGTTCTGC AGCAGGCCCT GGGGTCTGCA TGTGAGAGTA GGCCTGAGCT GAGGGGTGGA 
GGCGACTCTG CTCCTACCTT ATGGGGCCCT GATCCAGATC AGAATGGAGC TTCCAGACTC 
TTTAAGAGAT GGTCGCTCCC AACGGCACTG TCACCGGAGG CTGTGGCACT AGCTCTCCAG 
AAGCTCCATC AGGACGTGTG GAAGGCTCGG CAGGCCCGGG ATGACCTAAG GGACCAGGTC 
CAGAAGCTGG TGCAGCGCCT GACTGACACG GAGGCCCAGA AGAGCCAGGT TCACTCGGAG 
CTGCAGGACC TGCAGAGACA GCTCTCTCAG AGCCAGGAAG AGAAATCCAA GTGGGAAGGG 
AGGCAGAACT CCCTGGAGTC TGAGCTGAGG GACTTGCACG AGACCGCGGC ATCCTTACAG 
AGTCGCCTCC GGCAGGCAGA ACTGCAAAAA ATGGAAGCTC AGAATGATCG AGAGTTGCTT 
CAGGCATCCA AGGAGAAGCT GTCAGCCCAG GTTGAACATC TGCAAGCATG TGTTGCAGAA 
GCCCAGGCTC AGGCAGATGC AGCTGCTGTC CTGGAAGAAG ATCTGCGAAC TGCTCGCTCT 
GCACTGAAAC TGAAAAACGA GGAGCTAGAG AGTGAACGGG AGAGAGCCCA GGCTTTACAA 
GAACAGGGTG AGCTGAAGGT GGCTCAGGGG AAAGCTTTGC AGGAGAACCT AGCCCTGCTG 
GCACAGACCC TGTCTAACAG AGAGCGGGAG GTCGAGACGT TGCAGGCTGA GGTTCAGGAA 
CTGGAGAAGC AGCGGGAGAT GCAGAAGGCT GCTCTGGAGC TGCTCTCCCT CGACCTGAAG 
AAGCGCAGTC GGGAGGTAGA CCTGCAACAA GAACAGATTC AGGAGCTGGA GCAGTGCAGG 
TCAGTCTTAG AGCACTTGCC CATGGCCGTC CAAGAGCGAG AGCAGAAACT GAGTGTGCAG 
CGGGACCAGA TCAGGGAACT GGAGAACGAT CGGGAGGCCC AGAGGAGTGT CCTGGAACAC 
CAGCTTCTGG ACCTGGAGCA GAAGGCTCAA GTGATTGAGT CCCAGAGGGG ACAGATTCAG 
GATCTGAAAA AGCAACTGGG GACACTGGAA TGCCTGGCCC TGGAACTGGA GGAAAGCCAT 
CACAAAGTGG AGAGCCAACA GAAGATGATC ACAGAACTAG AGGGCCAGAG GGAAATGCAG 
AGGGTGGCTC TAACCCACCT CACACTGGAC CTGGAAGAAA GGAGCCAGGA GCTGCAGGCA 
CAAAGCAGCC AGCTCCATGA GCTGGAGAAC CATAGCACCC ATCTGGCAAA AGAGCTGCAG 
GAGAGGGACC AGGAGGTGAC GTCCCAACGC CAGCAGATCG ATGAGCTGCA GAAGCAGCAG 
GAACAGCTGG CTCAGGCCCT GGAGAGGAAA GGCCAGGAGC TGGTGCTGCA GAAGGAAAGG 
ATTCAGGTTC TGGAAGATCA GAGGACGCTG CAGACCAAGA TCTTAGAGGA GGACCTAGAA 
CAGATCAAGC ATTCCTTGAG AGAACGCAGC CAGGAGCTGG CCTCTCAGTG GCAGCTGGTG 
CATGAGCGTG CCGACGATGG AAAGAGCCCG AGTAAAGGCC AGCGAGGGAG CCTGGAGCAC 
TTGAAGCTGA TCCTGCGTGA TAAGGAGAAG GAGGTAGAGT GCCAGCAGGA GCGCATCCAG 
GAACTTCAGG GCCACATGGG CCAGCTGGAA CAGCAGCTGC AGGGTCTGCA CAGGAAGGTG 
GGGGAGACTA GCCTGCTCCT GACCCATCGA GAGCAGGAGA CAGCAACCCT GCAGCAGCAT 
CTCCAGGAAG CCAAGGAACA AGGGGAACTG AGAGAGCAGG TACTTCAGGG TCAGCTGGAG 
GAGGCCCAGA GAGATCTGGC CCAGAGGGAC CATGAACTAG AGACCCTCCG GCAGGAAAAA 
CAGCAGACCC AGGACCAGGA GGAAAGCATG AAGCTAAAGA CAAGTGCTCT GCAGGCCGCT 
CTGGAGCAGG CCCATGCGAC ACTGAAGGAG CGTCAGGGGG AGCTGGAAGA ACACAGGGAA 
CAGGTGCGAA GGCTCCAGGA AGAACTAGAA GTGGAGGGAC GGCAGGTACG GGCCCTGGAG 
GAGGTGTTGG GGGACCTAAG GGCTGAGTCT CGGGAGCACG AGAAGGCTGT GCTGGCCCTT 
CAACAACGGT GTGCTGAGCA GGCCCAGGAA CATGAGGCAG AAGCCAGGAC CCTGCAGGAC 
AGCTGGCTGC AGGCCCAGGC CACTCTCACA GAACAAGAAC AAGAGCTAGC GGCACTGCGA 
GCGGAAAACC AGTATTCCCG CCGACAGGAA GAGGCTGCAG TTAGCCAGGC AGAGGCGCTG 
CAGGAGGCCC TCAGCAAAGC CCAGGCTGCC CTGCAGGAGA AAGAGCAGAG TCTGCTTGAA 
CAGGCTGAGT TGAGCCACAC CCTGGAGGCC AGCACTGCCG CCTTGCAAGC TACCCTGGAC 
ACCTGCCAGG CAAGTGCCCG GCAGCTGGAG GAGGCCCTGA GGATTCGGGA AGGTGAGATC 
CAGGCCCAGG CTCTCCAACA CCACGAGGTC ACGCAGCACC TCCAGCAGGA ACTTTGCCAG 
AAGAAAGAAG AGCTGAGGCA GCTACTAGAG AAGGCTGGGG CCCGGAGGAG TCAAGAAAAT 
GGAATCCAAG AGAAGCAAAG TCTGGAGCAA GAGAGACAAG AGGAAACAAG GAGGCTCCTT 
GAGAGCCTAA AGGAGCTACA GCTGACTGTA GCCCAAAGGG AAGAAGAGAT TCTGATGCTG 
AGGGAGGCCT CGTCCCCACG CCACAGAGCC CTCCCAGCCG AGAAGCCAGC TCTGCAACCG 
CTCCCAGCAC AACAGGAGTT AGAGCGGCTG CAGACTGCCC TAAGGCAAAC AGAGGCCAGG 
GAGATTGAAT GGAGGGAGAA GGCTCAGGAC CTGGCTCTGT CCCTGGCCCA AAGCAAGGCC 
AGCATCAGCA GTCTGCAGGA GATAACCATG TTCCTACAAG CCTCTGTTCT AGAGCGGGAA 
TCAGAACAAC AAAGACTACA GGAGGAGTTG GTACTCAGCA GACAAGCTCT GGAAGAGCAG 
CAGTCAGGTG GCCCACACTC AACCAGCAGA GCAGACCAGG GACCAAAAGT GGGACAGGGA 
AGCCAGTCTG GAGAGGTAGA GACTGAGCCT AGTCCTGGAG TGGAGGAGAA GGAACGGTTG 
ACACAGAGGC TTGAACGTCT GCAGCAAGCG GTGGCAGAGC TGGAAGTTGA CCGAAGCAAG 
CTGCAGTGCC ACAATGCGCA GCTGCGGACC GCCTTGGAGC AGGTGGAACG AGAACGGAGG 
AAGCTGAAGA GGGATTCCGT GCGTGCATCC CGGGCAGGAT CACTGGAGGC CAGAGAAACT 
ATGACTTCAT CCCCCACACA GCAGGATGGG AGAGGCAGTC AGAGGGGTTC CTCGGACAGC 
GTGCTTGTGG TTGAGCTTCA GAGAGAGGTA GCCCTGCTGC GAGCCCAGCT GGCCTTGGAG 
CGAAAGCAGA GGCAGGACTA CATTGCACGG TCCGTGCAGA CCAGCCGTGA GCTAGCAGGC 
CTGCATCACA GCCTGTCTCA CTCCCTCCTC ACCGTGGCCC AGGCCCCTGA GGCCACTGTT 
CTGGAGGCTG AGACCCGAAA GCTGGATGAG TCTCTGAATC AGAGTCTGAC ATCCCCAGGG 
CCATGTTTGC TACATCCCAG TCTGGACACT ACTCAAAATA CCCACAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498862.1
CDS	75..7205
Translation	MAFRGCSMWMELERKFMFIQQGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKLTVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVAEAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDLEERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQTQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFLQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETEPSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR

Target ORF information:

RefSeq Version	XM_006498799.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498799.2

ORF Insert Sequence:

ATGGCTTTCC GTGGATGCAG TATGTGGATG GAATTAGAGA GAAAATTCAT GTTCATCCAG 
CAGGGGCTGA TCCCTCAGCG CTGGGAGAGT GTAGAAGAGC CCAACCTGGA ACAGTTGCTG 
ATCCGATTGG AGGAGGAGCA GCAGAGGTGT GAGAGTCTAG TGGAGGTGAA CACCGAGCTT 
CGGCTGCACA TGGAGAAAGC CGATGTGGTG AATAAAGCCT TGCAGGAGGA CGTGGAAAAA 
CTGACAGTGG ACTGGAGTCG GGCCCGAGAT GAGTTAGTGA GGAAGGAGAG CCAGTGGCGG 
ATGGAGCAGG AGTTCTTCAA GGGCTATTTG AGAGGTGAAC ACGGTCGTCT TCTCAATCTA 
TGGCGAGAGG TGGTAACCTT CCGACGTCAC TTCCTGAAAA TGAAGTCAGC TACTGACAGA 
GACCTGACAG AGCTAAAGGC TGAACACGCA AGGCTTTCAG GGTCCCTGCT GACCTGCTGT 
CTGCGCCTGA CCCTGAGAGC ACAGTCTCGG GAGTCCAGTG GATCTGGGAG AACAGAGGAG 
AGTGAGCCAG CCCGGCTGCT GCTGCTCGTC GCTAAGACCC AGGCGCTGGA GAAGGAAGCC 
CATGAGAAGA GCCAGGAGTT AATGCAGCTC AAGAGTCACG GGGATCTAGA GAAGGCAGAG 
CTGCAGGACC GAGTGACGGA ACTCTCTGCT CTGCTAACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAGA AGATGGTAAA GGCTCTAAGA GAAACAATGG AGATCCTGGA GACAAACCAT 
GCAGAGTTAA TGGAGCATGA GGCATCTCTC AGTAGGAATG CTCAAGAGGA AAAGTTGTCT 
CTGCAGCAGG TGATCAAGGC TATAACCCAG GCACTGGCCT CAGTGGAAGA AGAAGACACT 
GTGACCCAGT CCTCAGGCCA TGAGGACTCG CTGCAGTCAG ACTGTAATGG CCTTTCCCAG 
TTTGATCCCC AGGACCCAGA CAGGGCTCTT ACCCTGGTGC AGTCAGTGCT GACTCGGAGA 
CAGCAGGCTG TGCAGGACCT CAGGCAGCAG CTTTCAGGCT GCCAGGAGGC TATGAGCTTC 
TTGCAGCAGC AGCACGATCA GTGGGAGGAA GAGGGCAGGG CCCTGAGGGA AAAGCTACAG 
AAGCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGGCCTTCA GGGAGAGGTG 
GACTCTCTCA CTGGGGAGCG GGATGCTCTG GCAGGCCAGA CTGTGGACCT TCAGGGAGAG 
GTGGACTCTC TCAGCAGGGA GCGGGAGCTG CTTCAGAAGG CCAGAGGAGA GCTGCAGCAG 
CAACTGGAAG TGCTGGAGCA GGAAGCGTGG CGACTGCGGA GGATGAACAT GGAGCTGCAG 
CTGCAGGGGG ACTCAGCTCA AGGGGAGAAG CTGGAGCAGC AGGAGGAGTT GCACTTGGCT 
GTCCGGGAGA GGGAGCGCCT TCAGGAGACG CTGGTGGGCC TGGAAGCCAA GCAGTCAGAA 
TCACTAAGTG AGCTGCTCAC TCTTCGAGAA GCCCTGGAGT CAAGTCGCCT AGAAGGGGAG 
TTACTGAAGC AGGAGCGAGT GGAAGTGGCT GCAGCCTTGG CCAGGGCAGA ACAGTCCATT 
GTGGAGCTGT CAGGTTCTGA GAACAGCCTG AAGGCAGAGG TAGCTGATCT TCGAGCTGCA 
GCTGTCAAGC TTGGGGCCTT AAACGAGGCT TTGGCTTTAG ATAAAGTTGA ACTGAACCAG 
CAGCTTCTCC AGTTGGAGCA GGAGAACCAG TCTCTATGCA GCAGAGTGGA GGCCGCAGAG 
CAGCTGAGAA GCGCTTTGCA GGTGGACCTG GCGGAGGCAG AAAGGCGCAG GGAAGCCCTG 
TGGGAGAAGA AAACTCAGCT GGAGACTCAG CTGCAGAAGG CAGAGGAAGC GGGGGCTGAG 
CTACAGGCAG AGCTGAGGGG CACCCGAGAA GAAAAGGAAG AGCTTAAAGA TAAATTAAGT 
GAGGCACATC ACCAGCAAGA AACAGCTACA GCTCACCTAG AACAGCTGCA TCAGGACGCA 
GAAAGACAGG AAGAAACACT TGCTAGGGCA GTCCAGGAGA AGGAAGCCCT AGTACGGGAG 
AGGGCAGCTC TTGAGGTGCG CCTGCAGGCT GTGGAGCGGG ACCGACAGGA CCTCACTGAA 
CATGTCCTGG GGCTCAGGTC AGCCAAGGAG CAACTGGAGA GCAATCTATT TGAGGCCCAA 
CAGCAAAATT CTGTGATACA GGTCACCAAG GGGCAGCTGG AGGTCCAGAT TCAAACTATT 
ATCCAAGCCA AGGAAGTAAT TCAAGGGGAA GTGAAATGCC TGAAGCTGGA ACTGGATGCT 
GAGAGGACCC GGGCAGAGCA GGAGTGGGAT GCAGTAGCCA GGCAGCTAGC CCAAGCTGAG 
CAGGAGGGGC AAGCATCTCT GGAGCGACAG AAGGTGGCCC ACGAGGAGGA GGTGAACAGG 
CTGCAAGAGA AATGGGAGAA AGAGCGCTCC TGGCTGCAGC AGGAGCTGGA TAAGACCCTG 
GAAACCCTCG AGAGGGAGAG AGCAGAGCTG GAGACAAAGC TGAGAGAGCA GCAGACAGAA 
ATGGAAGCCA TCCGTGCACA GAGGGAAGAG GAACGAAGCC AGGCTGACAG CGCCCTGTAT 
CAAATGCAGC TAGAAACAGA GAAGGAGAGA GTATCCCTCC TGGAGACACT GCTGAGGACC 
CAGAAGGAGC TGGCAGATGC TAGTCAACAG CTGGAAAGGC TGAGACAGGA CATGAAGATT 
CAGAAGTTAA AGGAACAGGA GACCACTGGC ATGCTGCAGG CCCAGCTTCA GGAAACACAG 
CAGGAGCTGA AGGAGGCAGC CCAGCAGCAC AGGGACGACC TTGCTGCCTT CCAGAAGGAC 
AAGCTGGACC TGCAGAAGCA GGTAGAGGAC CTAATGTCTC AGCTGGTAGC CCACGATGAC 
TCCCAGAGGC TGGTGAAAGA AGAGATTGAA GAGAAGGTGA AAGTGGCTCA AGAATGCAGC 
CGGATTCAGA AGGAACTGGA GAAAGAGAAC GCCAGCCTGG CTCTGTCGCT GGTGGAAAAG 
GAAAAACGAC TTCTCATTTT ACAAGAAGCT GACTCTGTAA GACAGCAGGA GCTGAGCTCC 
TTGCGCCAGG ACATACAGGA GGCCCAGGAA GGGCAAAGAG AGCTGGGTGT ACAGGTGGAA 
CTATTGAGGC AGGAGGTGAA GGAAAAGGAG GCTGACTTTG TGGCCCGGGA AGCACAGCTG 
CTGGAGGAGT TGGAGGCCTC ACGGGTTGCA GAGCAGCAAC TTCGAGCGTC TTTGTGGGCC 
CAGGAAGCCA AGGCAACCCA GCTGCAGCTG CAGCTGCGCA GCACTGAGAG CCAGCTAGAG 
GCATTGGTTG CAGAACAACA GCCGGAGAAC CAGGCACAGG CACAGCTAGC TAGCCTCTGC 
TCTGTTCTGC AGCAGGCCCT GGGGTCTGCA TGTGAGAGTA GGCCTGAGCT GAGGGGTGGA 
GGCGACTCTG CTCCTACCTT ATGGGGCCCT GATCCAGATC AGAATGGAGC TTCCAGACTC 
TTTAAGAGAT GGTCGCTCCC AACGGCACTG TCACCGGAGG CTGTGGCACT AGCTCTCCAG 
AAGCTCCATC AGGACGTGTG GAAGGCTCGG CAGGCCCGGG ATGACCTAAG GGACCAGGTC 
CAGAAGCTGG TGCAGCGCCT GACTGACACG GAGGCCCAGA AGAGCCAGGT TCACTCGGAG 
CTGCAGGACC TGCAGAGACA GCTCTCTCAG AGCCAGGAAG AGAAATCCAA GTGGGAAGGG 
AGGCAGAACT CCCTGGAGTC TGAGCTGAGG GACTTGCACG AGACCGCGGC ATCCTTACAG 
AGTCGCCTCC GGCAGGCAGA ACTGCAAAAA ATGGAAGCTC AGAATGATCG AGAGTTGCTT 
CAGGCATCCA AGGAGAAGCT GTCAGCCCAG GTTGAACATC TGCAAGCATG TGTTGCAGAA 
GCCCAGGCTC AGGCAGATGC AGCTGCTGTC CTGGAAGAAG ATCTGCGAAC TGCTCGCTCT 
GCACTGAAAC TGAAAAACGA GGAGCTAGAG AGTGAACGGG AGAGAGCCCA GGCTTTACAA 
GAACAGGGTG AGCTGAAGGT GGCTCAGGGG AAAGCTTTGC AGGAGAACCT AGCCCTGCTG 
GCACAGACCC TGTCTAACAG AGAGCGGGAG GTCGAGACGT TGCAGGCTGA GGTTCAGGAA 
CTGGAGAAGC AGCGGGAGAT GCAGAAGGCT GCTCTGGAGC TGCTCTCCCT CGACCTGAAG 
AAGCGCAGTC GGGAGGTAGA CCTGCAACAA GAACAGATTC AGGAGCTGGA GCAGTGCAGG 
TCAGTCTTAG AGCACTTGCC CATGGCCGTC CAAGAGCGAG AGCAGAAACT GAGTGTGCAG 
CGGGACCAGA TCAGGGAACT GGAGAACGAT CGGGAGGCCC AGAGGAGTGT CCTGGAACAC 
CAGCTTCTGG ACCTGGAGCA GAAGGCTCAA GTGATTGAGT CCCAGAGGGG ACAGATTCAG 
GATCTGAAAA AGCAACTGGG GACACTGGAA TGCCTGGCCC TGGAACTGGA GGAAAGCCAT 
CACAAAGTGG AGAGCCAACA GAAGATGATC ACAGAACTAG AGGGCCAGAG GGAAATGCAG 
AGGGTGGCTC TAACCCACCT CACACTGGAC CTGGAAGAAA GGAGCCAGGA GCTGCAGGCA 
CAAAGCAGCC AGCTCCATGA GCTGGAGAAC CATAGCACCC ATCTGGCAAA AGAGCTGCAG 
GAGAGGGACC AGGAGGTGAC GTCCCAACGC CAGCAGATCG ATGAGCTGCA GAAGCAGCAG 
GAACAGCTGG CTCAGGCCCT GGAGAGGAAA GGCCAGGAGC TGGTGCTGCA GAAGGAAAGG 
ATTCAGGTTC TGGAAGATCA GAGGACGCTG CAGACCAAGA TCTTAGAGGA GGACCTAGAA 
CAGATCAAGC ATTCCTTGAG AGAACGCAGC CAGGAGCTGG CCTCTCAGTG GCAGCTGGTG 
CATGAGCGTG CCGACGATGG AAAGAGCCCG AGTAAAGGCC AGCGAGGGAG CCTGGAGCAC 
TTGAAGCTGA TCCTGCGTGA TAAGGAGAAG GAGGTAGAGT GCCAGCAGGA GCGCATCCAG 
GAACTTCAGG GCCACATGGG CCAGCTGGAA CAGCAGCTGC AGGGTCTGCA CAGGAAGGTG 
GGGGAGACTA GCCTGCTCCT GACCCATCGA GAGCAGGAGA CAGCAACCCT GCAGCAGCAT 
CTCCAGGAAG CCAAGGAACA AGGGGAACTG AGAGAGCAGG TACTTCAGGG TCAGCTGGAG 
GAGGCCCAGA GAGATCTGGC CCAGAGGGAC CATGAACTAG AGACCCTCCG GCAGGAAAAA 
CAGCAGACCC AGGACCAGGA GGAAAGCATG AAGCTAAAGA CAAGTGCTCT GCAGGCCGCT 
CTGGAGCAGG CCCATGCGAC ACTGAAGGAG CGTCAGGGGG AGCTGGAAGA ACACAGGGAA 
CAGGTGCGAA GGCTCCAGGA AGAACTAGAA GTGGAGGGAC GGCAGGTACG GGCCCTGGAG 
GAGGTGTTGG GGGACCTAAG GGCTGAGTCT CGGGAGCACG AGAAGGCTGT GCTGGCCCTT 
CAACAACGGT GTGCTGAGCA GGCCCAGGAA CATGAGGCAG AAGCCAGGAC CCTGCAGGAC 
AGCTGGCTGC AGGCCCAGGC CACTCTCACA GAACAAGAAC AAGAGCTAGC GGCACTGCGA 
GCGGAAAACC AGTATTCCCG CCGACAGGAA GAGGCTGCAG TTAGCCAGGC AGAGGCGCTG 
CAGGAGGCCC TCAGCAAAGC CCAGGCTGCC CTGCAGGAGA AAGAGCAGAG TCTGCTTGAA 
CAGGCTGAGT TGAGCCACAC CCTGGAGGCC AGCACTGCCG CCTTGCAAGC TACCCTGGAC 
ACCTGCCAGG CAAGTGCCCG GCAGCTGGAG GAGGCCCTGA GGATTCGGGA AGGTGAGATC 
CAGGCCCAGG CTCTCCAACA CCACGAGGTC ACGCAGCACC TCCAGCAGGA ACTTTGCCAG 
AAGAAAGAAG AGCTGAGGCA GCTACTAGAG AAGGCTGGGG CCCGGAGGAG TCAAGAAAAT 
GGAATCCAAG AGAAGCAAAG TCTGGAGCAA GAGAGACAAG AGGAAACAAG GAGGCTCCTT 
GAGAGCCTAA AGGAGCTACA GCTGACTGTA GCCCAAAGGG AAGAAGAGAT TCTGATGCTG 
AGGGAGGCCT CGTCCCCACG CCACAGAGCC CTCCCAGCCG AGAAGCCAGC TCTGCAACCG 
CTCCCAGCAC AACAGGAGTT AGAGCGGCTG CAGACTGCCC TAAGGCAAAC AGAGGCCAGG 
GAGATTGAAT GGAGGGAGAA GGCTCAGGAC CTGGCTCTGT CCCTGGCCCA AAGCAAGGCC 
AGCATCAGCA GTCTGCAGGA GATAACCATG TTCCTACAAG CCTCTGTTCT AGAGCGGGAA 
TCAGAACAAC AAAGACTACA GGAGGAGTTG GTACTCAGCA GACAAGCTCT GGAAGAGCAG 
CAGTCAGGTG GCCCACACTC AACCAGCAGA GCAGACCAGG GACCAAAAGT GGGACAGGGA 
AGCCAGTCTG GAGAGGTAGA GACTGAGCCT AGTCCTGGAG TGGAGGAGAA GGAACGGTTG 
ACACAGAGGC TTGAACGTCT GCAGCAAGCG GTGGCAGAGC TGGAAGTTGA CCGAAGCAAG 
CTGCAGTGCC ACAATGCGCA GCTGCGGACC GCCTTGGAGC AGGTGGAACG AGAACGGAGG 
AAGCTGAAGA GGGATTCCGT GCGTGCATCC CGGGCAGGAT CACTGGAGGC CAGAGAAACT 
ATGACTTCAT CCCCCACACA GCAGGATGGG AGAGGCAGTC AGAGGGGTTC CTCGGACAGC 
GTGCTTGTGG TTGAGCTTCA GAGAGAGGTA GCCCTGCTGC GAGCCCAGCT GGCCTTGGAG 
CGAAAGCAGA GGCAGGACTA CATTGCACGG TCCGTGCAGA CCAGCCGTGA GCTAGCAGGC 
CTGCATCACA GCCTGTCTCA CTCCCTCCTC ACCGTGGCCC AGGCCCCTGA GGCCACTGTT 
CTGGAGGCTG AGACCCGAAA GCTGGATGAG TCTCTGAATC AGAGTCTGAC ATCCCCAGGG 
CCATGTTTGC TACATCCCAG TCTGGACACT ACTCAAAATA CCCACAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep250 ( XM_017315701.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171190.1 Mus musculus centrosomal protein 250 (Cep250), transcript variant X9, mRNA.

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CloneID	OMu80076
Clone ID Related Accession (Same CDS sequence)	XM_017315701.1
Accession Version	XM_017315701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centrosome-associated protein CEP250 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGACCTTCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG GGAGCTGCTT CAGAAGGCCA GAGGAGAGCT GCAGCAGCAA 
CTGGAAGTGC TGGAGCAGGA AGCGTGGCGA CTGCGGAGGA TGAACATGGA GCTGCAGCTG 
CAGGGGGACT CAGCTCAAGG GGAGAAGCTG GAGCAGCAGG AGGAGTTGCA CTTGGCTGTC 
CGGGAGAGGG AGCGCCTTCA GGAGACGCTG GTGGGCCTGG AAGCCAAGCA GTCAGAATCA 
CTAAGTGAGC TGCTCACTCT TCGAGAAGCC CTGGAGTCAA GTCGCCTAGA AGGGGAGTTA 
CTGAAGCAGG AGCGAGTGGA AGTGGCTGCA GCCTTGGCCA GGGCAGAACA GTCCATTGTG 
GAGCTGTCAG GTTCTGAGAA CAGCCTGAAG GCAGAGGTAG CTGATCTTCG AGCTGCAGCT 
GTCAAGCTTG GGGCCTTAAA CGAGGCTTTG GCTTTAGATA AAGTTGAACT GAACCAGCAG 
CTTCTCCAGT TGGAGCAGGA GAACCAGTCT CTATGCAGCA GAGTGGAGGC CGCAGAGCAG 
CTGAGAAGCG CTTTGCAGGT GGACCTGGCG GAGGCAGAAA GGCGCAGGGA AGCCCTGTGG 
GAGAAGAAAA CTCAGCTGGA GACTCAGCTG CAGAAGGCAG AGGAAGCGGG GGCTGAGCTA 
CAGGCAGAGC TGAGGGGCAC CCGAGAAGAA AAGGAAGAGC TTAAAGATAA ATTAAGTGAG 
GCACATCACC AGCAAGAAAC AGCTACAGCT CACCTAGAAC AGCTGCATCA GGACGCAGAA 
AGACAGGAAG AAACACTTGC TAGGGCAGTC CAGGAGAAGG AAGCCCTAGT ACGGGAGAGG 
GCAGCTCTTG AGGTGCGCCT GCAGGCTGTG GAGCGGGACC GACAGGACCT CACTGAACAT 
GTCCTGGGGC TCAGGTCAGC CAAGGAGCAA CTGGAGAGCA ATCTATTTGA GGCCCAACAG 
CAAAATTCTG TGATACAGGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATTATC 
CAAGCCAAGG AAGTAATTCA AGGGGAAGTG AAATGCCTGA AGCTGGAACT GGATGCTGAG 
AGGACCCGGG CAGAGCAGGA GTGGGATGCA GTAGCCAGGC AGCTAGCCCA AGCTGAGCAG 
GAGGGGCAAG CATCTCTGGA GCGACAGAAG GTGGCCCACG AGGAGGAGGT GAACAGGCTG 
CAAGAGAAAT GGGAGAAAGA GCGCTCCTGG CTGCAGCAGG AGCTGGATAA GACCCTGGAA 
ACCCTCGAGA GGGAGAGAGC AGAGCTGGAG ACAAAGCTGA GAGAGCAGCA GACAGAAATG 
GAAGCCATCC GTGCACAGAG GGAAGAGGAA CGAAGCCAGG CTGACAGCGC CCTGTATCAA 
ATGCAGCTAG AAACAGAGAA GGAGAGAGTA TCCCTCCTGG AGACACTGCT GAGGACCCAG 
AAGGAGCTGG CAGATGCTAG TCAACAGCTG GAAAGGCTGA GACAGGACAT GAAGATTCAG 
AAGTTAAAGG AACAGGAGAC CACTGGCATG CTGCAGGCCC AGCTTCAGGA AACACAGCAG 
GAGCTGAAGG AGGCAGCCCA GCAGCACAGG GACGACCTTG CTGCCTTCCA GAAGGACAAG 
CTGGACCTGC AGAAGCAGGT AGAGGACCTA ATGTCTCAGC TGGTAGCCCA CGATGACTCC 
CAGAGGCTGG TGAAAGAAGA GATTGAAGAG AAGGTGAAAG TGGCTCAAGA ATGCAGCCGG 
ATTCAGAAGG AACTGGAGAA AGAGAACGCC AGCCTGGCTC TGTCGCTGGT GGAAAAGGAA 
AAACGACTTC TCATTTTACA AGAAGCTGAC TCTGTAAGAC AGCAGGAGCT GAGCTCCTTG 
CGCCAGGACA TACAGGAGGC CCAGGAAGGG CAAAGAGAGC TGGGTGTACA GGTGGAACTA 
TTGAGGCAGG AGGTGAAGGA AAAGGAGGCT GACTTTGTGG CCCGGGAAGC ACAGCTGCTG 
GAGGAGTTGG AGGCCTCACG GGTTGCAGAG CAGCAACTTC GAGCGTCTTT GTGGGCCCAG 
GAAGCCAAGG CAACCCAGCT GCAGCTGCAG CTGCGCAGCA CTGAGAGCCA GCTAGAGGCA 
TTGGTTGCAG AACAACAGCC GGAGAACCAG GCACAGGCAC AGCTAGCTAG CCTCTGCTCT 
GTTCTGCAGC AGGCCCTGGG GTCTGCATGT GAGAGTAGGC CTGAGCTGAG GGGTGGAGGC 
GACTCTGCTC CTACCTTATG GGGCCCTGAT CCAGATCAGA ATGGAGCTTC CAGACTCTTT 
AAGAGATGGT CGCTCCCAAC GGCACTGTCA CCGGAGGCTG TGGCACTAGC TCTCCAGAAG 
CTCCATCAGG ACGTGTGGAA GGCTCGGCAG GCCCGGGATG ACCTAAGGGA CCAGGTCCAG 
AAGCTGGTGC AGCGCCTGAC TGACACGGAG GCCCAGAAGA GCCAGGTTCA CTCGGAGCTG 
CAGGACCTGC AGAGACAGCT CTCTCAGAGC CAGGAAGGTT CCCCAGGGAT CCCACTGTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171190.1
CDS	301..4263
Translation	METGSPGLNMKPQSLQLVLEGQVLALQQQMAENQAASWRKLKNSQEAQKRQATLVRKLQAKVLQYRSWCQDLEKRLEATGGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKLTVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAGQTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQEADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEGSPGIPLW

Target ORF information:

RefSeq Version	XM_017315701.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centrosomal protein 250 (Cep250), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315701.1

ORF Insert Sequence:

ATGGAGACAG GGAGCCCCGG GTTGAACATG AAGCCCCAGT CCTTGCAGCT GGTGCTGGAG 
GGGCAGGTGC TGGCGCTGCA GCAGCAGATG GCCGAGAATC AGGCTGCCTC CTGGCGGAAA 
CTGAAGAACT CCCAAGAGGC CCAGAAGAGA CAAGCAACCC TCGTGAGGAA GCTGCAGGCC 
AAGGTTTTAC AGTACCGGAG CTGGTGTCAA GACCTGGAGA AGCGACTAGA AGCCACTGGG 
GGGCTGATCC CTCAGCGCTG GGAGAGTGTA GAAGAGCCCA ACCTGGAACA GTTGCTGATC 
CGATTGGAGG AGGAGCAGCA GAGGTGTGAG AGTCTAGTGG AGGTGAACAC CGAGCTTCGG 
CTGCACATGG AGAAAGCCGA TGTGGTGAAT AAAGCCTTGC AGGAGGACGT GGAAAAACTG 
ACAGTGGACT GGAGTCGGGC CCGAGATGAG TTAGTGAGGA AGGAGAGCCA GTGGCGGATG 
GAGCAGGAGT TCTTCAAGGG CTATTTGAGA GGTGAACACG GTCGTCTTCT CAATCTATGG 
CGAGAGGTGG TAACCTTCCG ACGTCACTTC CTGAAAATGA AGTCAGCTAC TGACAGAGAC 
CTGACAGAGC TAAAGGCTGA ACACGCAAGG CTTTCAGGGT CCCTGCTGAC CTGCTGTCTG 
CGCCTGACCC TGAGAGCACA GTCTCGGGAG TCCAGTGGAT CTGGGAGAAC AGAGGAGAGT 
GAGCCAGCCC GGCTGCTGCT GCTCGTCGCT AAGACCCAGG CGCTGGAGAA GGAAGCCCAT 
GAGAAGAGCC AGGAGTTAAT GCAGCTCAAG AGTCACGGGG ATCTAGAGAA GGCAGAGCTG 
CAGGACCGAG TGACGGAACT CTCTGCTCTG CTAACCCAGT CTCAGAAGCA AAATGAAGAT 
TATGAGAAGA TGGTAAAGGC TCTAAGAGAA ACAATGGAGA TCCTGGAGAC AAACCATGCA 
GAGTTAATGG AGCATGAGGC ATCTCTCAGT AGGAATGCTC AAGAGGAAAA GTTGTCTCTG 
CAGCAGGTGA TCAAGGCTAT AACCCAGGCA CTGGCCTCAG TGGAAGAAGA AGACACTGTG 
ACCCAGTCCT CAGGCCATGA GGACTCGCTG CAGTCAGACT GTAATGGCCT TTCCCAGTTT 
GATCCCCAGG ACCCAGACAG GGCTCTTACC CTGGTGCAGT CAGTGCTGAC TCGGAGACAG 
CAGGCTGTGC AGGACCTCAG GCAGCAGCTT TCAGGCTGCC AGGAGGCTAT GAGCTTCTTG 
CAGCAGCAGC ACGATCAGTG GGAGGAAGAG GGCAGGGCCC TGAGGGAAAA GCTACAGAAG 
CTCACTGGGG AGCGGGATGC TCTGGCAGGC CAGACTGTGG GCCTTCAGGG AGAGGTGGAC 
TCTCTCACTG GGGAGCGGGA TGCTCTGGCA GGCCAGACTG TGGACCTTCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG GGAGCTGCTT CAGAAGGCCA GAGGAGAGCT GCAGCAGCAA 
CTGGAAGTGC TGGAGCAGGA AGCGTGGCGA CTGCGGAGGA TGAACATGGA GCTGCAGCTG 
CAGGGGGACT CAGCTCAAGG GGAGAAGCTG GAGCAGCAGG AGGAGTTGCA CTTGGCTGTC 
CGGGAGAGGG AGCGCCTTCA GGAGACGCTG GTGGGCCTGG AAGCCAAGCA GTCAGAATCA 
CTAAGTGAGC TGCTCACTCT TCGAGAAGCC CTGGAGTCAA GTCGCCTAGA AGGGGAGTTA 
CTGAAGCAGG AGCGAGTGGA AGTGGCTGCA GCCTTGGCCA GGGCAGAACA GTCCATTGTG 
GAGCTGTCAG GTTCTGAGAA CAGCCTGAAG GCAGAGGTAG CTGATCTTCG AGCTGCAGCT 
GTCAAGCTTG GGGCCTTAAA CGAGGCTTTG GCTTTAGATA AAGTTGAACT GAACCAGCAG 
CTTCTCCAGT TGGAGCAGGA GAACCAGTCT CTATGCAGCA GAGTGGAGGC CGCAGAGCAG 
CTGAGAAGCG CTTTGCAGGT GGACCTGGCG GAGGCAGAAA GGCGCAGGGA AGCCCTGTGG 
GAGAAGAAAA CTCAGCTGGA GACTCAGCTG CAGAAGGCAG AGGAAGCGGG GGCTGAGCTA 
CAGGCAGAGC TGAGGGGCAC CCGAGAAGAA AAGGAAGAGC TTAAAGATAA ATTAAGTGAG 
GCACATCACC AGCAAGAAAC AGCTACAGCT CACCTAGAAC AGCTGCATCA GGACGCAGAA 
AGACAGGAAG AAACACTTGC TAGGGCAGTC CAGGAGAAGG AAGCCCTAGT ACGGGAGAGG 
GCAGCTCTTG AGGTGCGCCT GCAGGCTGTG GAGCGGGACC GACAGGACCT CACTGAACAT 
GTCCTGGGGC TCAGGTCAGC CAAGGAGCAA CTGGAGAGCA ATCTATTTGA GGCCCAACAG 
CAAAATTCTG TGATACAGGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATTATC 
CAAGCCAAGG AAGTAATTCA AGGGGAAGTG AAATGCCTGA AGCTGGAACT GGATGCTGAG 
AGGACCCGGG CAGAGCAGGA GTGGGATGCA GTAGCCAGGC AGCTAGCCCA AGCTGAGCAG 
GAGGGGCAAG CATCTCTGGA GCGACAGAAG GTGGCCCACG AGGAGGAGGT GAACAGGCTG 
CAAGAGAAAT GGGAGAAAGA GCGCTCCTGG CTGCAGCAGG AGCTGGATAA GACCCTGGAA 
ACCCTCGAGA GGGAGAGAGC AGAGCTGGAG ACAAAGCTGA GAGAGCAGCA GACAGAAATG 
GAAGCCATCC GTGCACAGAG GGAAGAGGAA CGAAGCCAGG CTGACAGCGC CCTGTATCAA 
ATGCAGCTAG AAACAGAGAA GGAGAGAGTA TCCCTCCTGG AGACACTGCT GAGGACCCAG 
AAGGAGCTGG CAGATGCTAG TCAACAGCTG GAAAGGCTGA GACAGGACAT GAAGATTCAG 
AAGTTAAAGG AACAGGAGAC CACTGGCATG CTGCAGGCCC AGCTTCAGGA AACACAGCAG 
GAGCTGAAGG AGGCAGCCCA GCAGCACAGG GACGACCTTG CTGCCTTCCA GAAGGACAAG 
CTGGACCTGC AGAAGCAGGT AGAGGACCTA ATGTCTCAGC TGGTAGCCCA CGATGACTCC 
CAGAGGCTGG TGAAAGAAGA GATTGAAGAG AAGGTGAAAG TGGCTCAAGA ATGCAGCCGG 
ATTCAGAAGG AACTGGAGAA AGAGAACGCC AGCCTGGCTC TGTCGCTGGT GGAAAAGGAA 
AAACGACTTC TCATTTTACA AGAAGCTGAC TCTGTAAGAC AGCAGGAGCT GAGCTCCTTG 
CGCCAGGACA TACAGGAGGC CCAGGAAGGG CAAAGAGAGC TGGGTGTACA GGTGGAACTA 
TTGAGGCAGG AGGTGAAGGA AAAGGAGGCT GACTTTGTGG CCCGGGAAGC ACAGCTGCTG 
GAGGAGTTGG AGGCCTCACG GGTTGCAGAG CAGCAACTTC GAGCGTCTTT GTGGGCCCAG 
GAAGCCAAGG CAACCCAGCT GCAGCTGCAG CTGCGCAGCA CTGAGAGCCA GCTAGAGGCA 
TTGGTTGCAG AACAACAGCC GGAGAACCAG GCACAGGCAC AGCTAGCTAG CCTCTGCTCT 
GTTCTGCAGC AGGCCCTGGG GTCTGCATGT GAGAGTAGGC CTGAGCTGAG GGGTGGAGGC 
GACTCTGCTC CTACCTTATG GGGCCCTGAT CCAGATCAGA ATGGAGCTTC CAGACTCTTT 
AAGAGATGGT CGCTCCCAAC GGCACTGTCA CCGGAGGCTG TGGCACTAGC TCTCCAGAAG 
CTCCATCAGG ACGTGTGGAA GGCTCGGCAG GCCCGGGATG ACCTAAGGGA CCAGGTCCAG 
AAGCTGGTGC AGCGCCTGAC TGACACGGAG GCCCAGAAGA GCCAGGTTCA CTCGGAGCTG 
CAGGACCTGC AGAGACAGCT CTCTCAGAGC CAGGAAGGTT CCCCAGGGAT CCCACTGTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Cep250
Entrez Gene ID	16328
Full Name	centrosomal protein 250
Synonyms	AW490617,B230210E21Rik,Cep2,Inmp
General protein information	Preferred Names centrosomal protein 250 Names centrosome-associated protein CEP250 250 kDa centrosomal protein C-Nap1 centrosomal Nek2-associated protein 1 intranuclear matrix protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H1

Gallus gallus (chicken)	LOC100858952	XP_003642497.2
Homo sapiens (human)	CEP250	NP_009117.2
Pan troglodytes (chimpanzee)	CEP250	XP_003316929.1
Mus musculus (house mouse)	Cep250	NP_001123471.1
Bos taurus (cattle)	CEP250	XP_005215062.1
Gallus gallus (chicken)	CEP250	XP_417323.4
	CEP250	XP_001099508.1
Canis lupus familiaris (dog)	CEP250	XP_542975.3
Rattus norvegicus (Norway rat)	Cep250	XP_006235436.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Cep250 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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