Nkx2-3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nkx2-3
Entrez Gene ID	18089
Full Name	NK2 homeobox 3
Synonyms	Nkx-2.3,Nkx2.3,tinman
General protein information	Preferred Names NK2 homeobox 3 Names homeobox protein Nkx-2.3 Drosophila NK2 transcription factor related, locus 3 NK2 transcription factor related, locus 3 homeobox protein NK-2 homolog 3 homeobox protein NK-2 homolog C nkx2-C
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 C3\|19 36.67 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008699.2	NP_032725.1	homeobox protein Nkx-2.3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NKX2-3	XP_003640037.1
Xenopus tropicalis (tropical clawed frog)	nkx2-3	XP_002937234.1
Rattus norvegicus (Norway rat)	Nkx2-3	NP_001101064.1
Homo sapiens (human)	NKX2-3	NP_660328.2
	NKX2-3	XP_001106216.2
Danio rerio (zebrafish)	nkx2.3	NP_571498.1
Pan troglodytes (chimpanzee)	NKX2-3	XP_001166828.2
Bos taurus (cattle)	NKX2-3	NP_001029848.1
Mus musculus (house mouse)	Nkx2-3	NP_032725.1

Gene Ontology
Bibliography

Related articles in PubMed

ARID3A is required for mammalian placenta development.
Rhee C, Edwards M, Dang C, Harris J, Brown M, Kim J, Tucker HO
Developmental biology422(2)83-91(2017 02)

Homeobox NKX2-3 promotes marginal-zone lymphomagenesis by activating B-cell receptor signalling and shaping lymphocyte dynamics.
Robles EF, Mena-Varas M, Barrio L, Merino-Cortes SV, Balogh P, Du MQ, Akasaka T, Parker A, Roa S, Panizo C, Martin-Guerrero I, Siebert R, Segura V, Agirre X, Macri-Pellizeri L, Aldaz B, Vilas-Zornoza A, Zhang S, Moody S, Calasanz MJ, Tousseyn T, Broccardo C, Brousset P, Campos-Sanchez E, Cobaleda C, Sanchez-Garcia I, Fernandez-Luna JL, Garcia-Mu?oz R, Pena E, Bellosillo B, Salar A, Baptista MJ, Hernandez-Rivas JM, Gonzalez M, Terol MJ, Climent J, Ferrandez A, Sagaert X, Melnick AM, Prosper F, Oscier DG, Carrasco YR, Dyer MJ, Martinez-Climent JA
Nature communications711889(2016 Jun)

Absence of Nkx2-3 homeodomain transcription factor reprograms the endothelial addressin preference for lymphocyte homing in Peyer's patches.
Kellermayer Z, Mihalj M, L?badi ?, Cz?mp?ly T, Lee M, O'Hara E, Butcher EC, Berta G, Balogh A, Arnold HH, Balogh P
Journal of immunology (Baltimore, Md. : 1950)193(10)5284-93(2014 Nov)

Wnt/?-catenin signaling maintains the mesenchymal precursor pool for murine sinus horn formation.
Norden J, Greulich F, Rudat C, Taketo MM, Kispert A
Circulation research109(6)e42-50(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nkx2-3 deficiency reprograms the endothelial addressin preference for lymphocyte homing in Peyer's patches.
Title: Absence of Nkx2-3 homeodomain transcription factor reprograms the endothelial addressin preference for lymphocyte homing in Peyer's patches.

The splenic vascular defects in Nkx2-3 deficiency include the generation of LYVE-1(+) cysts, comprised of endothelial cells without being committed along the LEC lineage.
Title: Absence of Nkx2-3 homeodomain transcription factor induces the formation of LYVE-1-positive endothelial cysts without lymphatic commitment in the spleen.

the organ-specific patterning of splenic vasculature is critically regulated by Nkx2-3, thereby profoundly affecting the lymphocyte homing mechanism and blood filtering capacity of the spleen in a tissue-specific manner.
Title: Transcription factor Nkx2-3 controls the vascular identity and lymphocyte homing in the spleen.

Phenotypic heterogeneity among different vascular elements within distinct anatomical regions of the spleen differentially depends on developmental factors such as lymphotoxin signaling or Nkx2.3, whereas the marginal sinus is controlled by both pathways.
Title: Distinct roles of lymphotoxin-beta signaling and the homeodomain transcription factor Nkx2.3 in the ontogeny of endothelial compartments in spleen.

Formation of various white pulp fibroblast subsets is differentially affected by the presence of Nkx2.3 activity, possibly also influencing their role in various immune functions linked with complement activation and deposition.
Title: Complex organizational defects of fibroblast architecture in the mouse spleen with Nkx2.3 homeodomain deficiency.

The following Nkx2-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nkx2-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02579	NM_008699.2 Latest version!	Mus musculus NK2 homeobox 3 (Nkx2-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nkx2-3 ( NM_008699.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032725.1 Mus musculus NK2 homeobox 3 (Nkx2-3), mRNA.

Price & Availability↑

CloneID	OMu02579
Clone ID Related Accession (Same CDS sequence)	NM_008699.2
Accession Version	NM_008699.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	homeobox protein Nkx-2.3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC072614.1. On or before Aug 30, 2004 this sequence version replaced XM_129268.4, NM_008699.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC072614.1, AK044871.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTTAC CAAGCCCGGT CACCTCCACC CCTTTCTCAG TCAAAGACAT TTTGAATCTG 
GAGCAGCAGC GGCACTTCCA CGGAGCTCAC TTGCAGGCGG AATTGGAGCA GCACTTCCAC 
TCGGCACCCT GCATGCTGGC CACGGCTGAA GGGACGCAGT TTTCCGATGC AGGGGAGGAG 
GACGAGGAAG AAGAGGGAGA GAAACTGTCC TATTTGAACT CACTAGCTGC TGCCGAGGGC 
CATGGAGATT CGGGTCTCTG TCCTCAGAGC TATGTCCATA CAGTTCTCCG AGACGCTTGC 
AGCGGGCCCA AGGAACAAGA AGAGGAGGTT GTGAGCGAAC GGAGCCAAAA AAGCTGTCAG 
TTGAAGAAGT CTCTGGAAGC GGCGGGAGAC TGTAAGACGA GCGAGGACGG CGAGAGGCCG 
AAGCCGCGGA GCCGCCGGAA GCCCCGGGTG CTCTTCTCGC AAGCTCAGGT CTTTGAGCTG 
GAGCGCAGGT TCAAGCAGCA GCGGTACCTG TCGGCGCCCG AGCGCGAGCA TCTCGCCAGC 
AGCCTGAAGC TCACGTCCAC GCAGGTGAAA ATCTGGTTCC AGAATCGCAG GTACAAGTGC 
AAGAGACAGC GGCAGGATAA GTCCCTGGAG CTGGGGACGC ACGCGCCGCC GCCGCCACCC 
CGCCGCGTGG CAGTGCCGGT GCTCGTGCGG GACGGCAAGC CGTGCGTCAC GCCCAGCGCA 
CAAACCTACG GCTCGCCCTA CGGCGTGGGC GCCGGCGCCT ACTCCTACAA CAGCTTCCCC 
GCCTACGGCT ACGGGAACTC GGCCGCCGCA GCCGCTGCAG CCGCCGCAGC CGCAGCAGCA 
GCGGCGGCTT ACAGCGGCAG CTACGGCTGC GCCTATCCGA CCGGTGGCGG CGGTGGTGGT 
GGCGGCACGG CCTCCGCGGC GACCACCGCC ATGCAACCCG CCTGCAGCGC CACCGGCGGT 
GGATCCTTTG TGAACGTGAG CAACCTGGGA GGCTTCGGCA GCGGCGGCGG CGCGCAACCC 
TTGCATCAAG GTGCTGCAGC CGGGTCCGCG TGCACGCAGG GGACTTTGCA GGGCATCAGG 
GCTTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032725.1
CDS	177..1265
Translation	MMLPSPVTSTPFSVKDILNLEQQRHFHGAHLQAELEQHFHSAPCMLATAEGTQFSDAGEEDEEEEGEKLSYLNSLAAAEGHGDSGLCPQSYVHTVLRDACSGPKEQEEEVVSERSQKSCQLKKSLEAAGDCKTSEDGERPKPRSRRKPRVLFSQAQVFELERRFKQQRYLSAPEREHLASSLKLTSTQVKIWFQNRRYKCKRQRQDKSLELGTHAPPPPPRRVAVPVLVRDGKPCVTPSAQTYGSPYGVGAGAYSYNSFPAYGYGNSAAAAAAAAAAAAAAAAYSGSYGCAYPTGGGGGGGGTASAATTAMQPACSATGGGSFVNVSNLGGFGSGGGAQPLHQGAAAGSACTQGTLQGIRAW

Target ORF information:

RefSeq Version	NM_008699.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NK2 homeobox 3 (Nkx2-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008699.2

ORF Insert Sequence:

ATGATGTTAC CAAGCCCGGT CACCTCCACC CCTTTCTCAG TCAAAGACAT TTTGAATCTG 
GAGCAGCAGC GGCACTTCCA CGGAGCTCAC TTGCAGGCGG AATTGGAGCA GCACTTCCAC 
TCGGCACCCT GCATGCTGGC CACGGCTGAA GGGACGCAGT TTTCCGATGC AGGGGAGGAG 
GACGAGGAAG AAGAGGGAGA GAAACTGTCC TATTTGAACT CACTAGCTGC TGCCGAGGGC 
CATGGAGATT CGGGTCTCTG TCCTCAGAGC TATGTCCATA CAGTTCTCCG AGACGCTTGC 
AGCGGGCCCA AGGAACAAGA AGAGGAGGTT GTGAGCGAAC GGAGCCAAAA AAGCTGTCAG 
TTGAAGAAGT CTCTGGAAGC GGCGGGAGAC TGTAAGACGA GCGAGGACGG CGAGAGGCCG 
AAGCCGCGGA GCCGCCGGAA GCCCCGGGTG CTCTTCTCGC AAGCTCAGGT CTTTGAGCTG 
GAGCGCAGGT TCAAGCAGCA GCGGTACCTG TCGGCGCCCG AGCGCGAGCA TCTCGCCAGC 
AGCCTGAAGC TCACGTCCAC GCAGGTGAAA ATCTGGTTCC AGAATCGCAG GTACAAGTGC 
AAGAGACAGC GGCAGGATAA GTCCCTGGAG CTGGGGACGC ACGCGCCGCC GCCGCCACCC 
CGCCGCGTGG CAGTGCCGGT GCTCGTGCGG GACGGCAAGC CGTGCGTCAC GCCCAGCGCA 
CAAACCTACG GCTCGCCCTA CGGCGTGGGC GCCGGCGCCT ACTCCTACAA CAGCTTCCCC 
GCCTACGGCT ACGGGAACTC GGCCGCCGCA GCCGCTGCAG CCGCCGCAGC CGCAGCAGCA 
GCGGCGGCTT ACAGCGGCAG CTACGGCTGC GCCTATCCGA CCGGTGGCGG CGGTGGTGGT 
GGCGGCACGG CCTCCGCGGC GACCACCGCC ATGCAACCCG CCTGCAGCGC CACCGGCGGT 
GGATCCTTTG TGAACGTGAG CAACCTGGGA GGCTTCGGCA GCGGCGGCGG CGCGCAACCC 
TTGCATCAAG GTGCTGCAGC CGGGTCCGCG TGCACGCAGG GGACTTTGCA GGGCATCAGG 
GCTTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ARID3A is required for mammalian placenta development.
Developmental biology422(2)83-91(2017 02)
Rhee C,Edwards M,Dang C,Harris J,Brown M,Kim J,Tucker HO

Homeobox NKX2-3 promotes marginal-zone lymphomagenesis by activating B-cell receptor signalling and shaping lymphocyte dynamics.
Nature communications711889(2016 Jun)
Robles EF,Mena-Varas M,Barrio L,Merino-Cortes SV,Balogh P,Du MQ,Akasaka T,Parker A,Roa S,Panizo C,Martin-Guerrero I,Siebert R,Segura V,Agirre X,Macri-Pellizeri L,Aldaz B,Vilas-Zornoza A,Zhang S,Moody S,Calasanz MJ,Tousseyn T,Broccardo C,Brousset P,Campos-Sanchez E,Cobaleda C,Sanchez-Garcia I,Fernandez-Luna JL,Garcia-Mu?oz R,Pena E,Bellosillo B,Salar A,Baptista MJ,Hernandez-Rivas JM,Gonzalez M,Terol MJ,Climent J,Ferrandez A,Sagaert X,Melnick AM,Prosper F,Oscier DG,Carrasco YR,Dyer MJ,Martinez-Climent JA

Absence of Nkx2-3 homeodomain transcription factor reprograms the endothelial addressin preference for lymphocyte homing in Peyer's patches.
Journal of immunology (Baltimore, Md. : 1950)193(10)5284-93(2014 Nov)
Kellermayer Z,Mihalj M,L?badi ?,Cz?mp?ly T,Lee M,O'Hara E,Butcher EC,Berta G,Balogh A,Arnold HH,Balogh P

Wnt/?-catenin signaling maintains the mesenchymal precursor pool for murine sinus horn formation.
Circulation research109(6)e42-50(2011 Sep)
Norden J,Greulich F,Rudat C,Taketo MM,Kispert A

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Nkx2-3 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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