Pip cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pip
Entrez Gene ID	18716
Full Name	prolactin induced protein
Synonyms	GCDFP-15,GP17,mSMGP
General protein information	Preferred Names prolactin induced protein Names prolactin-inducible protein homolog 14 kDa submandibular gland protein SMGP gross cystic disease fluid protein 15
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 B2.1\|6 20.05 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008843.3	NP_032869.2	prolactin-inducible protein homolog precursor
NM_008843.4	NP_032869.2	prolactin-inducible protein homolog precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PIP	XP_851087.1
	PIP	XP_001090222.1
Rattus norvegicus (Norway rat)	Pip	NP_073199.1
Homo sapiens (human)	PIP	NP_002643.1
Pan troglodytes (chimpanzee)	PIP	NP_001009170.1
Mus musculus (house mouse)	Pip	NP_032869.2

Gene Ontology
Bibliography

Related articles in PubMed

Prolactin-Inducible Protein: From Breast Cancer Biomarker to Immune Modulator-Novel Insights from Knockout Mice.
Ihedioha OC, Shiu RP, Uzonna JE, Myal Y
DNA and cell biology35(10)537-541(2016 Oct)

Cell-Specific Cre Strains For Genetic Manipulation in Salivary Glands.
Maruyama EO, Aure MH, Xie X, Myal Y, Gan L, Ovitt CE
PloS one11(1)e0146711(2016)

Deficiency of prolactin-inducible protein leads to impaired Th1 immune response and susceptibility to Leishmania major in mice.
Li J, Liu D, Mou Z, Ihedioha OC, Blanchard A, Jia P, Myal Y, Uzonna JE
European journal of immunology45(4)1082-91(2015 Apr)

Generation and initial characterization of the prolactin-inducible protein (PIP) null mouse: accompanying global changes in gene expression in the submandibular gland.
Blanchard A, Nistor A, Castaneda FE, Martin D, Hicks GG, Amara F, Shiu RP, Myal Y
Canadian journal of physiology and pharmacology87(10)859-72(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Recent work with Pip-deficient mice, linking PIP not only to a role in innate immunity but for the first time, providing evidence for a role in cell-mediated immunity. [Review]
Title: Prolactin-Inducible Protein: From Breast Cancer Biomarker to Immune Modulator-Novel Insights from Knockout Mice.

The findings implicate PIP as an important regulator of CD4(+) Th1-cell-mediated immunity.
Title: Deficiency of prolactin-inducible protein leads to impaired Th1 immune response and susceptibility to Leishmania major in mice.

possible function in host defense by promoting aggregation of oral bacteria
Title: Influence of mouse prolactin-inducible protein in saliva on the aggregation of oral bacteria.

these studies lend support to an immunomodulatory role for PIP in vivo and provide further insights into potentially novel signaling pathways and regulatory networks for PIP
Title: Generation and initial characterization of the prolactin-inducible protein (PIP) null mouse: accompanying global changes in gene expression in the submandibular gland.

examined the endogenous pattern of mouse SMGP/PIP (mSMGP/mPIP) gene expression in mid-and late-embryonic, and in early postnatal development
Title: Expression of the mouse homologue for the human GCDFP-15/PIP gene during pre- and early post-natal development.

The following Pip gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pip cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02890	NM_008843.4 Latest version!	Mus musculus prolactin induced protein (Pip), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OMu02890	NM_008843.3	Mus musculus prolactin induced protein (Pip), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pip ( NM_008843.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032869.2 Mus musculus prolactin induced protein (Pip), mRNA.

Price & Availability↑

CloneID	OMu02890
Clone ID Related Accession (Same CDS sequence)	NM_008843.3 , NM_008843.4
Accession Version	NM_008843.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Mus musculus(house mouse)
Product	prolactin-inducible protein homolog precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK138795.1. On Apr 6, 2007 this sequence version replaced NM_008843.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK138795.1, X02510.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849381 [ECO:0000350] ##Evidence-Data-END##

ATGCAGGGTC TCTCATTCAC ATTCAGTGCT GTGACACTCT TCTTGGTCCT ATGTCTGCAG 
CTGGGGATCA TTGAAAGCCA GGATGATGAA AATGTCCGAA AGCCACTTTT GATTGAAATA 
GATGTTCCAT CAACAGCACA GGAAAATCAG GAGATCACTG TGCAAGTTAC AGTTGAAACA 
CAATATAGAG AATGTATGGT GATCAAAGCT TACCTTGTAA GTAATGAACC AATGGAAGGT 
GCCTTCAACT ATGTACAAAC CCGCTGCCTC TGTAATGATC ATCCTATTAG ATTTTTCTGG 
GATATAATAA TCACAAGAAC TGTAACATTT GCAACAGTGA TTGATATTGT TCGAGAAAAG 
AATATCTGTC CTAATGATAT GGCAGTGGTG CCCATCACAG CAAACCGGTA CTATACTTAT 
AATACTGTGC GAATGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032869.2
CDS	32..472
Translation	MQGLSFTFSAVTLFLVLCLQLGIIESQDDENVRKPLLIEIDVPSTAQENQEITVQVTVETQYRECMVIKAYLVSNEPMEGAFNYVQTRCLCNDHPIRFFWDIIITRTVTFATVIDIVREKNICPNDMAVVPITANRYYTYNTVRMN

Target ORF information:

RefSeq Version	NM_008843.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolactin induced protein (Pip), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008843.3

ORF Insert Sequence:

ATGCAGGGTC TCTCATTCAC ATTCAGTGCT GTGACACTCT TCTTGGTCCT ATGTCTGCAG 
CTGGGGATCA TTGAAAGCCA GGATGATGAA AATGTCCGAA AGCCACTTTT GATTGAAATA 
GATGTTCCAT CAACAGCACA GGAAAATCAG GAGATCACTG TGCAAGTTAC AGTTGAAACA 
CAATATAGAG AATGTATGGT GATCAAAGCT TACCTTGTAA GTAATGAACC AATGGAAGGT 
GCCTTCAACT ATGTACAAAC CCGCTGCCTC TGTAATGATC ATCCTATTAG ATTTTTCTGG 
GATATAATAA TCACAAGAAC TGTAACATTT GCAACAGTGA TTGATATTGT TCGAGAAAAG 
AATATCTGTC CTAATGATAT GGCAGTGGTG CCCATCACAG CAAACCGGTA CTATACTTAT 
AATACTGTGC GAATGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pip ( NM_008843.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032869.2 Mus musculus prolactin induced protein (Pip), mRNA.

Price & Availability↑

CloneID	OMu02890
Clone ID Related Accession (Same CDS sequence)	NM_008843.3 , NM_008843.4
Accession Version	NM_008843.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	prolactin-inducible protein homolog precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK138795.1 and CK616212.1. On Feb 24, 2018 this sequence version replaced NM_008843.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028889.1, AK138795.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849381 [ECO:0000350] ##Evidence-Data-END##

ATGCAGGGTC TCTCATTCAC ATTCAGTGCT GTGACACTCT TCTTGGTCCT ATGTCTGCAG 
CTGGGGATCA TTGAAAGCCA GGATGATGAA AATGTCCGAA AGCCACTTTT GATTGAAATA 
GATGTTCCAT CAACAGCACA GGAAAATCAG GAGATCACTG TGCAAGTTAC AGTTGAAACA 
CAATATAGAG AATGTATGGT GATCAAAGCT TACCTTGTAA GTAATGAACC AATGGAAGGT 
GCCTTCAACT ATGTACAAAC CCGCTGCCTC TGTAATGATC ATCCTATTAG ATTTTTCTGG 
GATATAATAA TCACAAGAAC TGTAACATTT GCAACAGTGA TTGATATTGT TCGAGAAAAG 
AATATCTGTC CTAATGATAT GGCAGTGGTG CCCATCACAG CAAACCGGTA CTATACTTAT 
AATACTGTGC GAATGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032869.2
CDS	32..472
Translation	MQGLSFTFSAVTLFLVLCLQLGIIESQDDENVRKPLLIEIDVPSTAQENQEITVQVTVETQYRECMVIKAYLVSNEPMEGAFNYVQTRCLCNDHPIRFFWDIIITRTVTFATVIDIVREKNICPNDMAVVPITANRYYTYNTVRMN

Target ORF information:

RefSeq Version	NM_008843.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolactin induced protein (Pip), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008843.4

ORF Insert Sequence:

ATGCAGGGTC TCTCATTCAC ATTCAGTGCT GTGACACTCT TCTTGGTCCT ATGTCTGCAG 
CTGGGGATCA TTGAAAGCCA GGATGATGAA AATGTCCGAA AGCCACTTTT GATTGAAATA 
GATGTTCCAT CAACAGCACA GGAAAATCAG GAGATCACTG TGCAAGTTAC AGTTGAAACA 
CAATATAGAG AATGTATGGT GATCAAAGCT TACCTTGTAA GTAATGAACC AATGGAAGGT 
GCCTTCAACT ATGTACAAAC CCGCTGCCTC TGTAATGATC ATCCTATTAG ATTTTTCTGG 
GATATAATAA TCACAAGAAC TGTAACATTT GCAACAGTGA TTGATATTGT TCGAGAAAAG 
AATATCTGTC CTAATGATAT GGCAGTGGTG CCCATCACAG CAAACCGGTA CTATACTTAT 
AATACTGTGC GAATGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prolactin-Inducible Protein: From Breast Cancer Biomarker to Immune Modulator-Novel Insights from Knockout Mice.
DNA and cell biology35(10)537-541(2016 Oct)
Ihedioha OC,Shiu RP,Uzonna JE,Myal Y

Cell-Specific Cre Strains For Genetic Manipulation in Salivary Glands.
PloS one11(1)e0146711(2016)
Maruyama EO,Aure MH,Xie X,Myal Y,Gan L,Ovitt CE

Deficiency of prolactin-inducible protein leads to impaired Th1 immune response and susceptibility to Leishmania major in mice.
European journal of immunology45(4)1082-91(2015 Apr)
Li J,Liu D,Mou Z,Ihedioha OC,Blanchard A,Jia P,Myal Y,Uzonna JE

Generation and initial characterization of the prolactin-inducible protein (PIP) null mouse: accompanying global changes in gene expression in the submandibular gland.
Canadian journal of physiology and pharmacology87(10)859-72(2009 Oct)
Blanchard A,Nistor A,Castaneda FE,Martin D,Hicks GG,Amara F,Shiu RP,Myal Y