Nlrp1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nlrp1a
Entrez Gene ID	195046
Full Name	NLR family, pyrin domain containing 1A
Synonyms	CARD7,DEFCAP,Gm14,Gm15,NAC,Nalp1,Nalp1a,Nlrp1,PP1044
General protein information	Preferred Names NLR family, pyrin domain containing 1A Names NACHT, LRR and PYD domains-containing protein 1a NACHT, LRR and PYD containing protein 1 NACHT, leucine rich repeat and PYD containing 1 NACHT/LRR/pyrin domain-containing protein 1 NLR family, pyrin domain containing 1 NLR family, pyrin domain containing 1A, CAST/EiJ strain specific NLR family, pyrin domain containing 1A, PWK/PhJ strain specific NLR family, pyrin domain containing 1A, WSB/EiJ strain specific OTTMUSCASG00059399 OTTMUSPWKG00059389 OTTMUSWSBG00059289 caspase recruitment domain protein caspase recruitment domain-containing protein 7 death effector filament-forming Ced-4-like apoptosis protein nucleotide-binding domain and caspase recruitment domain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B4\|11 43.21 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017314354.1	XP_017169843.1	NACHT, LRR and PYD domains-containing protein 1a isoform X1
XM_017314354.2	XP_017169843.1	NACHT, LRR and PYD domains-containing protein 1a isoform X1
XM_017314355.1	XP_017169844.1	NACHT, LRR and PYD domains-containing protein 1a isoform X1
NM_001004142.2	NP_001004142.2	NACHT, LRR and PYD domains-containing protein 1a

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nlrp1a	NP_001139227.2
Mus musculus (house mouse)	Nlrp1a	NP_001004142.2

Gene Ontology
Bibliography

Related articles in PubMed

Mechanisms of NLRP1-Mediated Autoinflammatory Disease in Humans and Mice.
Yu CH, Moecking J, Geyer M, Masters SL
Journal of molecular biology430(2)142-152(2018 Jan)

Curcumin ameliorates neuropathic pain by down-regulating spinal IL-1? via suppressing astroglial NALP1 inflammasome and JAK2-STAT3 signalling.
Liu S, Li Q, Zhang MT, Mao-Ying QL, Hu LY, Wu GC, Mi WL, Wang YQ
Scientific reports628956(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the aim of this study was to evaluate the effects of chronic dexamethasone (DEX) treatment on nucleotide-binding oligomerization domain-like receptor pyrin domain-containing 1 (NLRP-1) inflammasome in male mice.
Title: Chronic glucocorticoids exposure enhances neurodegeneration in the frontal cortex and hippocampus via NLRP-1 inflammasome activation in male mice.

The findings indicate that NLRP1 inflammasome activation does not significantly contribute to acute neural injury in the murine model of moderate CCI injury.
Title: Nonessential Role for the NLRP1 Inflammasome Complex in a Murine Model of Traumatic Brain Injury.

NLRP1 is an innate immune sensor that functions in the context of metabolic stress to produce IL-18, preventing obesity and metabolic syndrome.
Title: IL-18 Production from the NLRP1 Inflammasome Prevents Obesity and Metabolic Syndrome.

these data identify NLRP1 as an essential mediator of the host immune response during IBD and cancer.
Title: The NLRP1 inflammasome attenuates colitis and colitis-associated tumorigenesis.

Data show that eicosanoid 15-deoxy-Delta(12,14)-PGJ2 (15d-PGJ2) and related cyclopentenone PGs inhibit caspase-1 activation by the NLR family leucine-rich repeat protein (NLRP)1 and NLRP3 inflammasomes.
Title: The cyclopentenone prostaglandin 15d-PGJ2 inhibits the NLRP1 and NLRP3 inflammasomes.

The following Nlrp1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nlrp1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19634	NM_001004142.2 Latest version!	Mus musculus NLR family, pyrin domain containing 1A (Nlrp1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$692.30 $989.00
OMu19634	XM_017314354.2 Latest version!	Mus musculus NLR family, pyrin domain containing 1A (Nlrp1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu19634	XM_017314355.1 Latest version!	Mus musculus NLR family, pyrin domain containing 1A (Nlrp1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$692.30 $989.00
View Old Accession Versions >>
OMu19634	XM_017314354.1	Mus musculus NLR family, pyrin domain containing 1A (Nlrp1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$692.30 $989.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nlrp1a ( NM_001004142.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001004142.2 Mus musculus NLR family, pyrin domain containing 1A (Nlrp1a), mRNA.

Price & Availability↑

CloneID	OMu19634
Clone ID Related Accession (Same CDS sequence)	XM_017314354.1 , XM_017314354.2 , XM_017314355.1 , NM_001004142.2
Accession Version	NM_001004142.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	NACHT, LRR and PYD domains-containing protein 1a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL662908.7, DQ117601.1 and AL596136.5. On Jun 23, 2009 this sequence version replaced NM_001004142.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: DQ117601.1, KC539856.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAAT CTCAGTCCAA GCAGGAAAGT AGCACAAAAG TGGCTCAGCA TGAAGGTCAA 
GAGGATGTGG ACCCAACCTT CAAAACAAAG AAACTTATGG AGGTGGAGCT AATGAAGCAC 
AGAGTGCAGC TAGAGAGGAA CTTGAAGCTA AGAACCTTTC CAGGAGCACG TACGAAGCAA 
GTAAAAGAGG CTCTTTACCC TCTTCTAACA TGGTCATCAA AAAGCAAGAA TCTGTTCCAA 
AATTTCACAA AACTACTACT CTTTAAAAAA TTGTGCCAAA GAGGCTCAGA AAACTTGGTC 
AGGGAAAGCT GGTATCCATG TGTACCAGAA GAAGAAGCAC ATATGATTGA CATCCAAGAT 
TTATTTGGCC CAAACTTAGG TACTCAGAAA AAGCCTCAAT TAGTCATAAT AGAAGGAGCT 
GCTGGGATTG GGAAGTCAAC ACTGGCCAGA CTTGTGAAGA GAGCCTGGAA GGAAGGCAAG 
CTTTACAGGA ATGACTTCCA TCATGTCTTC TTCTTCAGCT GCAGAGAGCT GGCCCAGTAT 
GAGCAGCTGA GTCTGGCTGA GCTCATAGTA CAAGGCCAGG AAGTGCCCAC AGCTCCCATT 
AGGCAGATCC TGTCTCACCC TGAGAAGTTG CTCTTCATTC TGGATGGCAT AGATGAGCCA 
GCATGGGTCT TAGCAGACCA GAATCCTGAG TTATGTCTTC ACTGGAGTCA AACCCAGCCT 
GTGCACACTC TGCTGGGCAG TTTACTTGGG AAATCCATCC TTCCTGGGGC TTCCTTCTTG 
CTCACAACTC GCACCACAGC TCTACAGAAA TTCATTCCTT CCCTGGAGCA GCCATGTCAA 
GTGGAAGTCC TAGGATTCAC TTTGTTTGAA CGGAAAAACT ATTTCTACAA ATATTTTGGG 
AAGAAAAAAG GTGGAGTTAC TACTTTTACA TTAGTTAAAT CAAACTCAGC GCTCCTGACT 
CTGTGTGAAG TTCCCTGGGT GTGCTGGTTG GTCTGCACTT GCCTGAAAAA GCAGATGGAG 
CAGGGTGGAG AGCTCTCACT GACCTCACAG ACCACCACAG CACTCTGCCT GAAATACCTT 
TCCCTGACGA TCCCAGGGCA GCACATGAGG ACCCAGCTCA GAGACCTCTG CTCACTGGCT 
GCTGAGGGGG TCTGCCAAAG AAGGACCCTG TTCAGTGAAA GCGATCTCTG TAAGCAAGGG 
TTAGATGAGC ATGCCATTGC CAGTTTCCTG AAGATTGGTG TCCTTCAAAA GCAGGCCAGC 
TCTCTGAGCT ACAGCTTTGC CCACTTGTGT CTTCAGGAGT TCTTTGCAGC AATGTCCTAC 
ATCTTGGATG ATAGTGAGGA AAGACATGCT GATATGAAAA ATGACAGAAT TGTGGAAACA 
CTGGTAGAAA GGTATGGAAG ACAAAACCTG TTTGAGGCAC CCACTGTGCG TTTTTTATTT 
GGTCTTTTGA GTAAAGAGGA ATTGAAAAAA ATAGAAAAGC TCTTTTCCTG CAGCCTTCAT 
GGAAAGACAA AGTTGAAACT ACTATGGCAC ATCCTAGGGA AATCCCAACC CCATCAACCA 
CCTTGCCTGG GTTTGCTCCA CTGTCTGTAT GAAAATCAGG ATATGGAGCT CCTGACACAC 
GTGATGCATG ATCTGCAAGG AACAATAGTG CCTGGACCAG ATGATTTGGC ACACACAGTG 
TTGCAGACAA ACGTGAAGCA CCTGGTGATA CAGACAGACA TGGACCTCAT GGTGGTTACT 
TTCTGCATTA AGTTCTGCTG TCACGTGAGG AGTCTTCAGC TGAACAGGAA GGTACAGCAG 
GGACATAAGT TTACGGCCCC TGGGATGGTT CTGTACAGAT GGACCCCAAT CACTGATGCC 
AGCTGGAAGA TTTTCTTCTC CAATCTTAAG TTAGCCAGAA ACCTGGAGGA GTTGGACCTA 
AGTGGAAATC CACTGAGCTA CTATGCAGTA CACAGTCTTT GTACGACCCT GAGAAAGCGT 
GGCTGCCAAC TAAAGACTCT GTGGCTTGTT GAATGCGGCC TCACATCCAC ATACTGCTCA 
CTTCTGGCCT CAGTACTCAG TGCCCGCTCC AGCCTGACTG AGCTAGACCT GCAGCTGAAT 
GACCTGGGTG ATGGTGGTGT GAAGATGTTG TGTGAGGGGC TCAGGAATCC TGCCTGCAAT 
CTCAGCATCC TGTGGCTGGA CCAGGCATCT CTCAGTGACC AAGTGATTGC AGAGCTCAGA 
ACTCTGGAGG CAAAGAATCC AAAGCTGCTC ATCTCCAGCA CATGGAAGCC ACATGTGATG 
GTCCCTACGA TGAACATGGA TAAAGAAGAA GTGGGTGATA GCCAGGCCCT ACTGAAGCAG 
CAGAGACAGC AGTCAGGAGA CAAACACATG GAACCTCTGG GGACTGAAGA TGAGTTCTGG 
GGCCCTACAG GACCTGTGAC TACTGAGGTG GTTGACAGAG AGAGGAACCT GTACCGAGTT 
CAGTTACCCA TGGCTGGTTC CTACCACTGT CCCAGTACAG GACTCCACTT TGTGGTGACA 
AGGGCAGTGA CAATCGAGAT TGAATTCTGT GCCTGGAGCC AATATCTCGA CAAGACTCCC 
TTGCAGCAGA GTCACATGGT GGTTGGACCT CTGTTTGACA TCAAGGCTGA GCAAGGAGCT 
GTGACTGCAG TGTACCTCCC TCATTTTGTG GCCCTCCAAG AGGGAATCGT GGACAGCTCC 
TTGTTCCATG TTGCCCACTT TCAAGAACAT GGGATGGTCC TAGAAACACC AGCAAGGGTG 
GAACAGCATT ACGCAGTACT GGAAAACCCA AGCTTCTCCC CAATGGGGAT TCTACTGAGA 
ATGATTCCGG CTGTCGGACA CTTCATCCCC ATCACTTCCA CCACACTGAT CTACTATCAC 
CTCTATCTTG AAGACGTCAC CTTTCACCTT TACCTGGTCC CCAATGACTG CAGTATTCGA 
AAGGCCATTG ATGATGAAGA AATGAAGTTT CAGTTTGTGC GCATAAACAA GCCACCCCCA 
GTAGACGCTC TTTATCTTGG TTCCCGCTAT ATCGTGTCTT CCTCCAAATT GGTGGAAATT 
ATCCCAAAGG AACTAGAGCT GTGCTACCGA AGCCCTGGGG AATCCCAGCT CTTCTCTGAG 
ATCGACATTG GGCACATGGA TTCAGAGATT AAGCTGCAAA TCAAAGACAA GAGGCACATG 
AATCTCAAAT GGGAGGCCTT GCTGAAACCA GGGGATCTCA GACCTGCACT GCCCAAGATT 
GCTACAGCCC CCAAAGATGC TCCTTCCTTG CTGCACTTCA TGGACCAGCA TCGGGAGCAG 
CTGGTGGCCC GAGTGACATC TGTAGATCCT CTCTTGGACA AGCTGCATGG TCTGGTGCTG 
AGTGAAGATT CCTATGAAGT AGTGCGGTCT GAGACCACAA ACCAAGACAA GATGAGGAAG 
CTCTTCAGCC TCAGCCGGTC CTGGAGCTGG GATTGCAAAG ACCAATTCTA CCAAGCTCTG 
AAGGAGACCC ATCCTCACCT GGTCATGGAT ATACTTGAGA AGTTGGGTGG GGTCTCTGTG 
AAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004142.2
CDS	219..3767
Translation	MEESQSKQESSTKVAQHEGQEDVDPTFKTKKLMEVELMKHRVQLERNLKLRTFPGARTKQVKEALYPLLTWSSKSKNLFQNFTKLLLFKKLCQRGSENLVRESWYPCVPEEEAHMIDIQDLFGPNLGTQKKPQLVIIEGAAGIGKSTLARLVKRAWKEGKLYRNDFHHVFFFSCRELAQYEQLSLAELIVQGQEVPTAPIRQILSHPEKLLFILDGIDEPAWVLADQNPELCLHWSQTQPVHTLLGSLLGKSILPGASFLLTTRTTALQKFIPSLEQPCQVEVLGFTLFERKNYFYKYFGKKKGGVTTFTLVKSNSALLTLCEVPWVCWLVCTCLKKQMEQGGELSLTSQTTTALCLKYLSLTIPGQHMRTQLRDLCSLAAEGVCQRRTLFSESDLCKQGLDEHAIASFLKIGVLQKQASSLSYSFAHLCLQEFFAAMSYILDDSEERHADMKNDRIVETLVERYGRQNLFEAPTVRFLFGLLSKEELKKIEKLFSCSLHGKTKLKLLWHILGKSQPHQPPCLGLLHCLYENQDMELLTHVMHDLQGTIVPGPDDLAHTVLQTNVKHLVIQTDMDLMVVTFCIKFCCHVRSLQLNRKVQQGHKFTAPGMVLYRWTPITDASWKIFFSNLKLARNLEELDLSGNPLSYYAVHSLCTTLRKRGCQLKTLWLVECGLTSTYCSLLASVLSARSSLTELDLQLNDLGDGGVKMLCEGLRNPACNLSILWLDQASLSDQVIAELRTLEAKNPKLLISSTWKPHVMVPTMNMDKEEVGDSQALLKQQRQQSGDKHMEPLGTEDEFWGPTGPVTTEVVDRERNLYRVQLPMAGSYHCPSTGLHFVVTRAVTIEIEFCAWSQYLDKTPLQQSHMVVGPLFDIKAEQGAVTAVYLPHFVALQEGIVDSSLFHVAHFQEHGMVLETPARVEQHYAVLENPSFSPMGILLRMIPAVGHFIPITSTTLIYYHLYLEDVTFHLYLVPNDCSIRKAIDDEEMKFQFVRINKPPPVDALYLGSRYIVSSSKLVEIIPKELELCYRSPGESQLFSEIDIGHMDSEIKLQIKDKRHMNLKWEALLKPGDLRPALPKIATAPKDAPSLLHFMDQHREQLVARVTSVDPLLDKLHGLVLSEDSYEVVRSETTNQDKMRKLFSLSRSWSWDCKDQFYQALKETHPHLVMDILEKLGGVSVKS

Target ORF information:

RefSeq Version	NM_001004142.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, pyrin domain containing 1A (Nlrp1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004142.2

ORF Insert Sequence:

ATGGAAGAAT CTCAGTCCAA GCAGGAAAGT AGCACAAAAG TGGCTCAGCA TGAAGGTCAA 
GAGGATGTGG ACCCAACCTT CAAAACAAAG AAACTTATGG AGGTGGAGCT AATGAAGCAC 
AGAGTGCAGC TAGAGAGGAA CTTGAAGCTA AGAACCTTTC CAGGAGCACG TACGAAGCAA 
GTAAAAGAGG CTCTTTACCC TCTTCTAACA TGGTCATCAA AAAGCAAGAA TCTGTTCCAA 
AATTTCACAA AACTACTACT CTTTAAAAAA TTGTGCCAAA GAGGCTCAGA AAACTTGGTC 
AGGGAAAGCT GGTATCCATG TGTACCAGAA GAAGAAGCAC ATATGATTGA CATCCAAGAT 
TTATTTGGCC CAAACTTAGG TACTCAGAAA AAGCCTCAAT TAGTCATAAT AGAAGGAGCT 
GCTGGGATTG GGAAGTCAAC ACTGGCCAGA CTTGTGAAGA GAGCCTGGAA GGAAGGCAAG 
CTTTACAGGA ATGACTTCCA TCATGTCTTC TTCTTCAGCT GCAGAGAGCT GGCCCAGTAT 
GAGCAGCTGA GTCTGGCTGA GCTCATAGTA CAAGGCCAGG AAGTGCCCAC AGCTCCCATT 
AGGCAGATCC TGTCTCACCC TGAGAAGTTG CTCTTCATTC TGGATGGCAT AGATGAGCCA 
GCATGGGTCT TAGCAGACCA GAATCCTGAG TTATGTCTTC ACTGGAGTCA AACCCAGCCT 
GTGCACACTC TGCTGGGCAG TTTACTTGGG AAATCCATCC TTCCTGGGGC TTCCTTCTTG 
CTCACAACTC GCACCACAGC TCTACAGAAA TTCATTCCTT CCCTGGAGCA GCCATGTCAA 
GTGGAAGTCC TAGGATTCAC TTTGTTTGAA CGGAAAAACT ATTTCTACAA ATATTTTGGG 
AAGAAAAAAG GTGGAGTTAC TACTTTTACA TTAGTTAAAT CAAACTCAGC GCTCCTGACT 
CTGTGTGAAG TTCCCTGGGT GTGCTGGTTG GTCTGCACTT GCCTGAAAAA GCAGATGGAG 
CAGGGTGGAG AGCTCTCACT GACCTCACAG ACCACCACAG CACTCTGCCT GAAATACCTT 
TCCCTGACGA TCCCAGGGCA GCACATGAGG ACCCAGCTCA GAGACCTCTG CTCACTGGCT 
GCTGAGGGGG TCTGCCAAAG AAGGACCCTG TTCAGTGAAA GCGATCTCTG TAAGCAAGGG 
TTAGATGAGC ATGCCATTGC CAGTTTCCTG AAGATTGGTG TCCTTCAAAA GCAGGCCAGC 
TCTCTGAGCT ACAGCTTTGC CCACTTGTGT CTTCAGGAGT TCTTTGCAGC AATGTCCTAC 
ATCTTGGATG ATAGTGAGGA AAGACATGCT GATATGAAAA ATGACAGAAT TGTGGAAACA 
CTGGTAGAAA GGTATGGAAG ACAAAACCTG TTTGAGGCAC CCACTGTGCG TTTTTTATTT 
GGTCTTTTGA GTAAAGAGGA ATTGAAAAAA ATAGAAAAGC TCTTTTCCTG CAGCCTTCAT 
GGAAAGACAA AGTTGAAACT ACTATGGCAC ATCCTAGGGA AATCCCAACC CCATCAACCA 
CCTTGCCTGG GTTTGCTCCA CTGTCTGTAT GAAAATCAGG ATATGGAGCT CCTGACACAC 
GTGATGCATG ATCTGCAAGG AACAATAGTG CCTGGACCAG ATGATTTGGC ACACACAGTG 
TTGCAGACAA ACGTGAAGCA CCTGGTGATA CAGACAGACA TGGACCTCAT GGTGGTTACT 
TTCTGCATTA AGTTCTGCTG TCACGTGAGG AGTCTTCAGC TGAACAGGAA GGTACAGCAG 
GGACATAAGT TTACGGCCCC TGGGATGGTT CTGTACAGAT GGACCCCAAT CACTGATGCC 
AGCTGGAAGA TTTTCTTCTC CAATCTTAAG TTAGCCAGAA ACCTGGAGGA GTTGGACCTA 
AGTGGAAATC CACTGAGCTA CTATGCAGTA CACAGTCTTT GTACGACCCT GAGAAAGCGT 
GGCTGCCAAC TAAAGACTCT GTGGCTTGTT GAATGCGGCC TCACATCCAC ATACTGCTCA 
CTTCTGGCCT CAGTACTCAG TGCCCGCTCC AGCCTGACTG AGCTAGACCT GCAGCTGAAT 
GACCTGGGTG ATGGTGGTGT GAAGATGTTG TGTGAGGGGC TCAGGAATCC TGCCTGCAAT 
CTCAGCATCC TGTGGCTGGA CCAGGCATCT CTCAGTGACC AAGTGATTGC AGAGCTCAGA 
ACTCTGGAGG CAAAGAATCC AAAGCTGCTC ATCTCCAGCA CATGGAAGCC ACATGTGATG 
GTCCCTACGA TGAACATGGA TAAAGAAGAA GTGGGTGATA GCCAGGCCCT ACTGAAGCAG 
CAGAGACAGC AGTCAGGAGA CAAACACATG GAACCTCTGG GGACTGAAGA TGAGTTCTGG 
GGCCCTACAG GACCTGTGAC TACTGAGGTG GTTGACAGAG AGAGGAACCT GTACCGAGTT 
CAGTTACCCA TGGCTGGTTC CTACCACTGT CCCAGTACAG GACTCCACTT TGTGGTGACA 
AGGGCAGTGA CAATCGAGAT TGAATTCTGT GCCTGGAGCC AATATCTCGA CAAGACTCCC 
TTGCAGCAGA GTCACATGGT GGTTGGACCT CTGTTTGACA TCAAGGCTGA GCAAGGAGCT 
GTGACTGCAG TGTACCTCCC TCATTTTGTG GCCCTCCAAG AGGGAATCGT GGACAGCTCC 
TTGTTCCATG TTGCCCACTT TCAAGAACAT GGGATGGTCC TAGAAACACC AGCAAGGGTG 
GAACAGCATT ACGCAGTACT GGAAAACCCA AGCTTCTCCC CAATGGGGAT TCTACTGAGA 
ATGATTCCGG CTGTCGGACA CTTCATCCCC ATCACTTCCA CCACACTGAT CTACTATCAC 
CTCTATCTTG AAGACGTCAC CTTTCACCTT TACCTGGTCC CCAATGACTG CAGTATTCGA 
AAGGCCATTG ATGATGAAGA AATGAAGTTT CAGTTTGTGC GCATAAACAA GCCACCCCCA 
GTAGACGCTC TTTATCTTGG TTCCCGCTAT ATCGTGTCTT CCTCCAAATT GGTGGAAATT 
ATCCCAAAGG AACTAGAGCT GTGCTACCGA AGCCCTGGGG AATCCCAGCT CTTCTCTGAG 
ATCGACATTG GGCACATGGA TTCAGAGATT AAGCTGCAAA TCAAAGACAA GAGGCACATG 
AATCTCAAAT GGGAGGCCTT GCTGAAACCA GGGGATCTCA GACCTGCACT GCCCAAGATT 
GCTACAGCCC CCAAAGATGC TCCTTCCTTG CTGCACTTCA TGGACCAGCA TCGGGAGCAG 
CTGGTGGCCC GAGTGACATC TGTAGATCCT CTCTTGGACA AGCTGCATGG TCTGGTGCTG 
AGTGAAGATT CCTATGAAGT AGTGCGGTCT GAGACCACAA ACCAAGACAA GATGAGGAAG 
CTCTTCAGCC TCAGCCGGTC CTGGAGCTGG GATTGCAAAG ACCAATTCTA CCAAGCTCTG 
AAGGAGACCC ATCCTCACCT GGTCATGGAT ATACTTGAGA AGTTGGGTGG GGTCTCTGTG 
AAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp1a ( XM_017314354.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169843.1 Mus musculus NLR family, pyrin domain containing 1A (Nlrp1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19634
Clone ID Related Accession (Same CDS sequence)	XM_017314354.1 , XM_017314354.2 , XM_017314355.1 , NM_001004142.2
Accession Version	XM_017314354.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NACHT, LRR and PYD domains-containing protein 1a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT CTCAGTCCAA GCAGGAAAGT AGCACAAAAG TGGCTCAGCA TGAAGGTCAA 
GAGGATGTGG ACCCAACCTT CAAAACAAAG AAACTTATGG AGGTGGAGCT AATGAAGCAC 
AGAGTGCAGC TAGAGAGGAA CTTGAAGCTA AGAACCTTTC CAGGAGCACG TACGAAGCAA 
GTAAAAGAGG CTCTTTACCC TCTTCTAACA TGGTCATCAA AAAGCAAGAA TCTGTTCCAA 
AATTTCACAA AACTACTACT CTTTAAAAAA TTGTGCCAAA GAGGCTCAGA AAACTTGGTC 
AGGGAAAGCT GGTATCCATG TGTACCAGAA GAAGAAGCAC ATATGATTGA CATCCAAGAT 
TTATTTGGCC CAAACTTAGG TACTCAGAAA AAGCCTCAAT TAGTCATAAT AGAAGGAGCT 
GCTGGGATTG GGAAGTCAAC ACTGGCCAGA CTTGTGAAGA GAGCCTGGAA GGAAGGCAAG 
CTTTACAGGA ATGACTTCCA TCATGTCTTC TTCTTCAGCT GCAGAGAGCT GGCCCAGTAT 
GAGCAGCTGA GTCTGGCTGA GCTCATAGTA CAAGGCCAGG AAGTGCCCAC AGCTCCCATT 
AGGCAGATCC TGTCTCACCC TGAGAAGTTG CTCTTCATTC TGGATGGCAT AGATGAGCCA 
GCATGGGTCT TAGCAGACCA GAATCCTGAG TTATGTCTTC ACTGGAGTCA AACCCAGCCT 
GTGCACACTC TGCTGGGCAG TTTACTTGGG AAATCCATCC TTCCTGGGGC TTCCTTCTTG 
CTCACAACTC GCACCACAGC TCTACAGAAA TTCATTCCTT CCCTGGAGCA GCCATGTCAA 
GTGGAAGTCC TAGGATTCAC TTTGTTTGAA CGGAAAAACT ATTTCTACAA ATATTTTGGG 
AAGAAAAAAG GTGGAGTTAC TACTTTTACA TTAGTTAAAT CAAACTCAGC GCTCCTGACT 
CTGTGTGAAG TTCCCTGGGT GTGCTGGTTG GTCTGCACTT GCCTGAAAAA GCAGATGGAG 
CAGGGTGGAG AGCTCTCACT GACCTCACAG ACCACCACAG CACTCTGCCT GAAATACCTT 
TCCCTGACGA TCCCAGGGCA GCACATGAGG ACCCAGCTCA GAGACCTCTG CTCACTGGCT 
GCTGAGGGGG TCTGCCAAAG AAGGACCCTG TTCAGTGAAA GCGATCTCTG TAAGCAAGGG 
TTAGATGAGC ATGCCATTGC CAGTTTCCTG AAGATTGGTG TCCTTCAAAA GCAGGCCAGC 
TCTCTGAGCT ACAGCTTTGC CCACTTGTGT CTTCAGGAGT TCTTTGCAGC AATGTCCTAC 
ATCTTGGATG ATAGTGAGGA AAGACATGCT GATATGAAAA ATGACAGAAT TGTGGAAACA 
CTGGTAGAAA GGTATGGAAG ACAAAACCTG TTTGAGGCAC CCACTGTGCG TTTTTTATTT 
GGTCTTTTGA GTAAAGAGGA ATTGAAAAAA ATAGAAAAGC TCTTTTCCTG CAGCCTTCAT 
GGAAAGACAA AGTTGAAACT ACTATGGCAC ATCCTAGGGA AATCCCAACC CCATCAACCA 
CCTTGCCTGG GTTTGCTCCA CTGTCTGTAT GAAAATCAGG ATATGGAGCT CCTGACACAC 
GTGATGCATG ATCTGCAAGG AACAATAGTG CCTGGACCAG ATGATTTGGC ACACACAGTG 
TTGCAGACAA ACGTGAAGCA CCTGGTGATA CAGACAGACA TGGACCTCAT GGTGGTTACT 
TTCTGCATTA AGTTCTGCTG TCACGTGAGG AGTCTTCAGC TGAACAGGAA GGTACAGCAG 
GGACATAAGT TTACGGCCCC TGGGATGGTT CTGTACAGAT GGACCCCAAT CACTGATGCC 
AGCTGGAAGA TTTTCTTCTC CAATCTTAAG TTAGCCAGAA ACCTGGAGGA GTTGGACCTA 
AGTGGAAATC CACTGAGCTA CTATGCAGTA CACAGTCTTT GTACGACCCT GAGAAAGCGT 
GGCTGCCAAC TAAAGACTCT GTGGCTTGTT GAATGCGGCC TCACATCCAC ATACTGCTCA 
CTTCTGGCCT CAGTACTCAG TGCCCGCTCC AGCCTGACTG AGCTAGACCT GCAGCTGAAT 
GACCTGGGTG ATGGTGGTGT GAAGATGTTG TGTGAGGGGC TCAGGAATCC TGCCTGCAAT 
CTCAGCATCC TGTGGCTGGA CCAGGCATCT CTCAGTGACC AAGTGATTGC AGAGCTCAGA 
ACTCTGGAGG CAAAGAATCC AAAGCTGCTC ATCTCCAGCA CATGGAAGCC ACATGTGATG 
GTCCCTACGA TGAACATGGA TAAAGAAGAA GTGGGTGATA GCCAGGCCCT ACTGAAGCAG 
CAGAGACAGC AGTCAGGAGA CAAACACATG GAACCTCTGG GGACTGAAGA TGAGTTCTGG 
GGCCCTACAG GACCTGTGAC TACTGAGGTG GTTGACAGAG AGAGGAACCT GTACCGAGTT 
CAGTTACCCA TGGCTGGTTC CTACCACTGT CCCAGTACAG GACTCCACTT TGTGGTGACA 
AGGGCAGTGA CAATCGAGAT TGAATTCTGT GCCTGGAGCC AATATCTCGA CAAGACTCCC 
TTGCAGCAGA GTCACATGGT GGTTGGACCT CTGTTTGACA TCAAGGCTGA GCAAGGAGCT 
GTGACTGCAG TGTACCTCCC TCATTTTGTG GCCCTCCAAG AGGGAATCGT GGACAGCTCC 
TTGTTCCATG TTGCCCACTT TCAAGAACAT GGGATGGTCC TAGAAACACC AGCAAGGGTG 
GAACAGCATT ACGCAGTACT GGAAAACCCA AGCTTCTCCC CAATGGGGAT TCTACTGAGA 
ATGATTCCGG CTGTCGGACA CTTCATCCCC ATCACTTCCA CCACACTGAT CTACTATCAC 
CTCTATCTTG AAGACGTCAC CTTTCACCTT TACCTGGTCC CCAATGACTG CAGTATTCGA 
AAGGCCATTG ATGATGAAGA AATGAAGTTT CAGTTTGTGC GCATAAACAA GCCACCCCCA 
GTAGACGCTC TTTATCTTGG TTCCCGCTAT ATCGTGTCTT CCTCCAAATT GGTGGAAATT 
ATCCCAAAGG AACTAGAGCT GTGCTACCGA AGCCCTGGGG AATCCCAGCT CTTCTCTGAG 
ATCGACATTG GGCACATGGA TTCAGAGATT AAGCTGCAAA TCAAAGACAA GAGGCACATG 
AATCTCAAAT GGGAGGCCTT GCTGAAACCA GGGGATCTCA GACCTGCACT GCCCAAGATT 
GCTACAGCCC CCAAAGATGC TCCTTCCTTG CTGCACTTCA TGGACCAGCA TCGGGAGCAG 
CTGGTGGCCC GAGTGACATC TGTAGATCCT CTCTTGGACA AGCTGCATGG TCTGGTGCTG 
AGTGAAGATT CCTATGAAGT AGTGCGGTCT GAGACCACAA ACCAAGACAA GATGAGGAAG 
CTCTTCAGCC TCAGCCGGTC CTGGAGCTGG GATTGCAAAG ACCAATTCTA CCAAGCTCTG 
AAGGAGACCC ATCCTCACCT GGTCATGGAT ATACTTGAGA AGTTGGGTGG GGTCTCTGTG 
AAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169843.1
CDS	485..4033
Translation	MEESQSKQESSTKVAQHEGQEDVDPTFKTKKLMEVELMKHRVQLERNLKLRTFPGARTKQVKEALYPLLTWSSKSKNLFQNFTKLLLFKKLCQRGSENLVRESWYPCVPEEEAHMIDIQDLFGPNLGTQKKPQLVIIEGAAGIGKSTLARLVKRAWKEGKLYRNDFHHVFFFSCRELAQYEQLSLAELIVQGQEVPTAPIRQILSHPEKLLFILDGIDEPAWVLADQNPELCLHWSQTQPVHTLLGSLLGKSILPGASFLLTTRTTALQKFIPSLEQPCQVEVLGFTLFERKNYFYKYFGKKKGGVTTFTLVKSNSALLTLCEVPWVCWLVCTCLKKQMEQGGELSLTSQTTTALCLKYLSLTIPGQHMRTQLRDLCSLAAEGVCQRRTLFSESDLCKQGLDEHAIASFLKIGVLQKQASSLSYSFAHLCLQEFFAAMSYILDDSEERHADMKNDRIVETLVERYGRQNLFEAPTVRFLFGLLSKEELKKIEKLFSCSLHGKTKLKLLWHILGKSQPHQPPCLGLLHCLYENQDMELLTHVMHDLQGTIVPGPDDLAHTVLQTNVKHLVIQTDMDLMVVTFCIKFCCHVRSLQLNRKVQQGHKFTAPGMVLYRWTPITDASWKIFFSNLKLARNLEELDLSGNPLSYYAVHSLCTTLRKRGCQLKTLWLVECGLTSTYCSLLASVLSARSSLTELDLQLNDLGDGGVKMLCEGLRNPACNLSILWLDQASLSDQVIAELRTLEAKNPKLLISSTWKPHVMVPTMNMDKEEVGDSQALLKQQRQQSGDKHMEPLGTEDEFWGPTGPVTTEVVDRERNLYRVQLPMAGSYHCPSTGLHFVVTRAVTIEIEFCAWSQYLDKTPLQQSHMVVGPLFDIKAEQGAVTAVYLPHFVALQEGIVDSSLFHVAHFQEHGMVLETPARVEQHYAVLENPSFSPMGILLRMIPAVGHFIPITSTTLIYYHLYLEDVTFHLYLVPNDCSIRKAIDDEEMKFQFVRINKPPPVDALYLGSRYIVSSSKLVEIIPKELELCYRSPGESQLFSEIDIGHMDSEIKLQIKDKRHMNLKWEALLKPGDLRPALPKIATAPKDAPSLLHFMDQHREQLVARVTSVDPLLDKLHGLVLSEDSYEVVRSETTNQDKMRKLFSLSRSWSWDCKDQFYQALKETHPHLVMDILEKLGGVSVKS

Target ORF information:

RefSeq Version	XM_017314354.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, pyrin domain containing 1A (Nlrp1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314354.1

ORF Insert Sequence:

ATGGAAGAAT CTCAGTCCAA GCAGGAAAGT AGCACAAAAG TGGCTCAGCA TGAAGGTCAA 
GAGGATGTGG ACCCAACCTT CAAAACAAAG AAACTTATGG AGGTGGAGCT AATGAAGCAC 
AGAGTGCAGC TAGAGAGGAA CTTGAAGCTA AGAACCTTTC CAGGAGCACG TACGAAGCAA 
GTAAAAGAGG CTCTTTACCC TCTTCTAACA TGGTCATCAA AAAGCAAGAA TCTGTTCCAA 
AATTTCACAA AACTACTACT CTTTAAAAAA TTGTGCCAAA GAGGCTCAGA AAACTTGGTC 
AGGGAAAGCT GGTATCCATG TGTACCAGAA GAAGAAGCAC ATATGATTGA CATCCAAGAT 
TTATTTGGCC CAAACTTAGG TACTCAGAAA AAGCCTCAAT TAGTCATAAT AGAAGGAGCT 
GCTGGGATTG GGAAGTCAAC ACTGGCCAGA CTTGTGAAGA GAGCCTGGAA GGAAGGCAAG 
CTTTACAGGA ATGACTTCCA TCATGTCTTC TTCTTCAGCT GCAGAGAGCT GGCCCAGTAT 
GAGCAGCTGA GTCTGGCTGA GCTCATAGTA CAAGGCCAGG AAGTGCCCAC AGCTCCCATT 
AGGCAGATCC TGTCTCACCC TGAGAAGTTG CTCTTCATTC TGGATGGCAT AGATGAGCCA 
GCATGGGTCT TAGCAGACCA GAATCCTGAG TTATGTCTTC ACTGGAGTCA AACCCAGCCT 
GTGCACACTC TGCTGGGCAG TTTACTTGGG AAATCCATCC TTCCTGGGGC TTCCTTCTTG 
CTCACAACTC GCACCACAGC TCTACAGAAA TTCATTCCTT CCCTGGAGCA GCCATGTCAA 
GTGGAAGTCC TAGGATTCAC TTTGTTTGAA CGGAAAAACT ATTTCTACAA ATATTTTGGG 
AAGAAAAAAG GTGGAGTTAC TACTTTTACA TTAGTTAAAT CAAACTCAGC GCTCCTGACT 
CTGTGTGAAG TTCCCTGGGT GTGCTGGTTG GTCTGCACTT GCCTGAAAAA GCAGATGGAG 
CAGGGTGGAG AGCTCTCACT GACCTCACAG ACCACCACAG CACTCTGCCT GAAATACCTT 
TCCCTGACGA TCCCAGGGCA GCACATGAGG ACCCAGCTCA GAGACCTCTG CTCACTGGCT 
GCTGAGGGGG TCTGCCAAAG AAGGACCCTG TTCAGTGAAA GCGATCTCTG TAAGCAAGGG 
TTAGATGAGC ATGCCATTGC CAGTTTCCTG AAGATTGGTG TCCTTCAAAA GCAGGCCAGC 
TCTCTGAGCT ACAGCTTTGC CCACTTGTGT CTTCAGGAGT TCTTTGCAGC AATGTCCTAC 
ATCTTGGATG ATAGTGAGGA AAGACATGCT GATATGAAAA ATGACAGAAT TGTGGAAACA 
CTGGTAGAAA GGTATGGAAG ACAAAACCTG TTTGAGGCAC CCACTGTGCG TTTTTTATTT 
GGTCTTTTGA GTAAAGAGGA ATTGAAAAAA ATAGAAAAGC TCTTTTCCTG CAGCCTTCAT 
GGAAAGACAA AGTTGAAACT ACTATGGCAC ATCCTAGGGA AATCCCAACC CCATCAACCA 
CCTTGCCTGG GTTTGCTCCA CTGTCTGTAT GAAAATCAGG ATATGGAGCT CCTGACACAC 
GTGATGCATG ATCTGCAAGG AACAATAGTG CCTGGACCAG ATGATTTGGC ACACACAGTG 
TTGCAGACAA ACGTGAAGCA CCTGGTGATA CAGACAGACA TGGACCTCAT GGTGGTTACT 
TTCTGCATTA AGTTCTGCTG TCACGTGAGG AGTCTTCAGC TGAACAGGAA GGTACAGCAG 
GGACATAAGT TTACGGCCCC TGGGATGGTT CTGTACAGAT GGACCCCAAT CACTGATGCC 
AGCTGGAAGA TTTTCTTCTC CAATCTTAAG TTAGCCAGAA ACCTGGAGGA GTTGGACCTA 
AGTGGAAATC CACTGAGCTA CTATGCAGTA CACAGTCTTT GTACGACCCT GAGAAAGCGT 
GGCTGCCAAC TAAAGACTCT GTGGCTTGTT GAATGCGGCC TCACATCCAC ATACTGCTCA 
CTTCTGGCCT CAGTACTCAG TGCCCGCTCC AGCCTGACTG AGCTAGACCT GCAGCTGAAT 
GACCTGGGTG ATGGTGGTGT GAAGATGTTG TGTGAGGGGC TCAGGAATCC TGCCTGCAAT 
CTCAGCATCC TGTGGCTGGA CCAGGCATCT CTCAGTGACC AAGTGATTGC AGAGCTCAGA 
ACTCTGGAGG CAAAGAATCC AAAGCTGCTC ATCTCCAGCA CATGGAAGCC ACATGTGATG 
GTCCCTACGA TGAACATGGA TAAAGAAGAA GTGGGTGATA GCCAGGCCCT ACTGAAGCAG 
CAGAGACAGC AGTCAGGAGA CAAACACATG GAACCTCTGG GGACTGAAGA TGAGTTCTGG 
GGCCCTACAG GACCTGTGAC TACTGAGGTG GTTGACAGAG AGAGGAACCT GTACCGAGTT 
CAGTTACCCA TGGCTGGTTC CTACCACTGT CCCAGTACAG GACTCCACTT TGTGGTGACA 
AGGGCAGTGA CAATCGAGAT TGAATTCTGT GCCTGGAGCC AATATCTCGA CAAGACTCCC 
TTGCAGCAGA GTCACATGGT GGTTGGACCT CTGTTTGACA TCAAGGCTGA GCAAGGAGCT 
GTGACTGCAG TGTACCTCCC TCATTTTGTG GCCCTCCAAG AGGGAATCGT GGACAGCTCC 
TTGTTCCATG TTGCCCACTT TCAAGAACAT GGGATGGTCC TAGAAACACC AGCAAGGGTG 
GAACAGCATT ACGCAGTACT GGAAAACCCA AGCTTCTCCC CAATGGGGAT TCTACTGAGA 
ATGATTCCGG CTGTCGGACA CTTCATCCCC ATCACTTCCA CCACACTGAT CTACTATCAC 
CTCTATCTTG AAGACGTCAC CTTTCACCTT TACCTGGTCC CCAATGACTG CAGTATTCGA 
AAGGCCATTG ATGATGAAGA AATGAAGTTT CAGTTTGTGC GCATAAACAA GCCACCCCCA 
GTAGACGCTC TTTATCTTGG TTCCCGCTAT ATCGTGTCTT CCTCCAAATT GGTGGAAATT 
ATCCCAAAGG AACTAGAGCT GTGCTACCGA AGCCCTGGGG AATCCCAGCT CTTCTCTGAG 
ATCGACATTG GGCACATGGA TTCAGAGATT AAGCTGCAAA TCAAAGACAA GAGGCACATG 
AATCTCAAAT GGGAGGCCTT GCTGAAACCA GGGGATCTCA GACCTGCACT GCCCAAGATT 
GCTACAGCCC CCAAAGATGC TCCTTCCTTG CTGCACTTCA TGGACCAGCA TCGGGAGCAG 
CTGGTGGCCC GAGTGACATC TGTAGATCCT CTCTTGGACA AGCTGCATGG TCTGGTGCTG 
AGTGAAGATT CCTATGAAGT AGTGCGGTCT GAGACCACAA ACCAAGACAA GATGAGGAAG 
CTCTTCAGCC TCAGCCGGTC CTGGAGCTGG GATTGCAAAG ACCAATTCTA CCAAGCTCTG 
AAGGAGACCC ATCCTCACCT GGTCATGGAT ATACTTGAGA AGTTGGGTGG GGTCTCTGTG 
AAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp1a ( XM_017314354.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169843.1 Mus musculus NLR family, pyrin domain containing 1A (Nlrp1a), transcript variant X1, mRNA.

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CloneID	OMu19634
Clone ID Related Accession (Same CDS sequence)	XM_017314354.1 , XM_017314354.2 , XM_017314355.1 , NM_001004142.2
Accession Version	XM_017314354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NACHT, LRR and PYD domains-containing protein 1a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT CTCAGTCCAA GCAGGAAAGT AGCACAAAAG TGGCTCAGCA TGAAGGTCAA 
GAGGATGTGG ACCCAACCTT CAAAACAAAG AAACTTATGG AGGTGGAGCT AATGAAGCAC 
AGAGTGCAGC TAGAGAGGAA CTTGAAGCTA AGAACCTTTC CAGGAGCACG TACGAAGCAA 
GTAAAAGAGG CTCTTTACCC TCTTCTAACA TGGTCATCAA AAAGCAAGAA TCTGTTCCAA 
AATTTCACAA AACTACTACT CTTTAAAAAA TTGTGCCAAA GAGGCTCAGA AAACTTGGTC 
AGGGAAAGCT GGTATCCATG TGTACCAGAA GAAGAAGCAC ATATGATTGA CATCCAAGAT 
TTATTTGGCC CAAACTTAGG TACTCAGAAA AAGCCTCAAT TAGTCATAAT AGAAGGAGCT 
GCTGGGATTG GGAAGTCAAC ACTGGCCAGA CTTGTGAAGA GAGCCTGGAA GGAAGGCAAG 
CTTTACAGGA ATGACTTCCA TCATGTCTTC TTCTTCAGCT GCAGAGAGCT GGCCCAGTAT 
GAGCAGCTGA GTCTGGCTGA GCTCATAGTA CAAGGCCAGG AAGTGCCCAC AGCTCCCATT 
AGGCAGATCC TGTCTCACCC TGAGAAGTTG CTCTTCATTC TGGATGGCAT AGATGAGCCA 
GCATGGGTCT TAGCAGACCA GAATCCTGAG TTATGTCTTC ACTGGAGTCA AACCCAGCCT 
GTGCACACTC TGCTGGGCAG TTTACTTGGG AAATCCATCC TTCCTGGGGC TTCCTTCTTG 
CTCACAACTC GCACCACAGC TCTACAGAAA TTCATTCCTT CCCTGGAGCA GCCATGTCAA 
GTGGAAGTCC TAGGATTCAC TTTGTTTGAA CGGAAAAACT ATTTCTACAA ATATTTTGGG 
AAGAAAAAAG GTGGAGTTAC TACTTTTACA TTAGTTAAAT CAAACTCAGC GCTCCTGACT 
CTGTGTGAAG TTCCCTGGGT GTGCTGGTTG GTCTGCACTT GCCTGAAAAA GCAGATGGAG 
CAGGGTGGAG AGCTCTCACT GACCTCACAG ACCACCACAG CACTCTGCCT GAAATACCTT 
TCCCTGACGA TCCCAGGGCA GCACATGAGG ACCCAGCTCA GAGACCTCTG CTCACTGGCT 
GCTGAGGGGG TCTGCCAAAG AAGGACCCTG TTCAGTGAAA GCGATCTCTG TAAGCAAGGG 
TTAGATGAGC ATGCCATTGC CAGTTTCCTG AAGATTGGTG TCCTTCAAAA GCAGGCCAGC 
TCTCTGAGCT ACAGCTTTGC CCACTTGTGT CTTCAGGAGT TCTTTGCAGC AATGTCCTAC 
ATCTTGGATG ATAGTGAGGA AAGACATGCT GATATGAAAA ATGACAGAAT TGTGGAAACA 
CTGGTAGAAA GGTATGGAAG ACAAAACCTG TTTGAGGCAC CCACTGTGCG TTTTTTATTT 
GGTCTTTTGA GTAAAGAGGA ATTGAAAAAA ATAGAAAAGC TCTTTTCCTG CAGCCTTCAT 
GGAAAGACAA AGTTGAAACT ACTATGGCAC ATCCTAGGGA AATCCCAACC CCATCAACCA 
CCTTGCCTGG GTTTGCTCCA CTGTCTGTAT GAAAATCAGG ATATGGAGCT CCTGACACAC 
GTGATGCATG ATCTGCAAGG AACAATAGTG CCTGGACCAG ATGATTTGGC ACACACAGTG 
TTGCAGACAA ACGTGAAGCA CCTGGTGATA CAGACAGACA TGGACCTCAT GGTGGTTACT 
TTCTGCATTA AGTTCTGCTG TCACGTGAGG AGTCTTCAGC TGAACAGGAA GGTACAGCAG 
GGACATAAGT TTACGGCCCC TGGGATGGTT CTGTACAGAT GGACCCCAAT CACTGATGCC 
AGCTGGAAGA TTTTCTTCTC CAATCTTAAG TTAGCCAGAA ACCTGGAGGA GTTGGACCTA 
AGTGGAAATC CACTGAGCTA CTATGCAGTA CACAGTCTTT GTACGACCCT GAGAAAGCGT 
GGCTGCCAAC TAAAGACTCT GTGGCTTGTT GAATGCGGCC TCACATCCAC ATACTGCTCA 
CTTCTGGCCT CAGTACTCAG TGCCCGCTCC AGCCTGACTG AGCTAGACCT GCAGCTGAAT 
GACCTGGGTG ATGGTGGTGT GAAGATGTTG TGTGAGGGGC TCAGGAATCC TGCCTGCAAT 
CTCAGCATCC TGTGGCTGGA CCAGGCATCT CTCAGTGACC AAGTGATTGC AGAGCTCAGA 
ACTCTGGAGG CAAAGAATCC AAAGCTGCTC ATCTCCAGCA CATGGAAGCC ACATGTGATG 
GTCCCTACGA TGAACATGGA TAAAGAAGAA GTGGGTGATA GCCAGGCCCT ACTGAAGCAG 
CAGAGACAGC AGTCAGGAGA CAAACACATG GAACCTCTGG GGACTGAAGA TGAGTTCTGG 
GGCCCTACAG GACCTGTGAC TACTGAGGTG GTTGACAGAG AGAGGAACCT GTACCGAGTT 
CAGTTACCCA TGGCTGGTTC CTACCACTGT CCCAGTACAG GACTCCACTT TGTGGTGACA 
AGGGCAGTGA CAATCGAGAT TGAATTCTGT GCCTGGAGCC AATATCTCGA CAAGACTCCC 
TTGCAGCAGA GTCACATGGT GGTTGGACCT CTGTTTGACA TCAAGGCTGA GCAAGGAGCT 
GTGACTGCAG TGTACCTCCC TCATTTTGTG GCCCTCCAAG AGGGAATCGT GGACAGCTCC 
TTGTTCCATG TTGCCCACTT TCAAGAACAT GGGATGGTCC TAGAAACACC AGCAAGGGTG 
GAACAGCATT ACGCAGTACT GGAAAACCCA AGCTTCTCCC CAATGGGGAT TCTACTGAGA 
ATGATTCCGG CTGTCGGACA CTTCATCCCC ATCACTTCCA CCACACTGAT CTACTATCAC 
CTCTATCTTG AAGACGTCAC CTTTCACCTT TACCTGGTCC CCAATGACTG CAGTATTCGA 
AAGGCCATTG ATGATGAAGA AATGAAGTTT CAGTTTGTGC GCATAAACAA GCCACCCCCA 
GTAGACGCTC TTTATCTTGG TTCCCGCTAT ATCGTGTCTT CCTCCAAATT GGTGGAAATT 
ATCCCAAAGG AACTAGAGCT GTGCTACCGA AGCCCTGGGG AATCCCAGCT CTTCTCTGAG 
ATCGACATTG GGCACATGGA TTCAGAGATT AAGCTGCAAA TCAAAGACAA GAGGCACATG 
AATCTCAAAT GGGAGGCCTT GCTGAAACCA GGGGATCTCA GACCTGCACT GCCCAAGATT 
GCTACAGCCC CCAAAGATGC TCCTTCCTTG CTGCACTTCA TGGACCAGCA TCGGGAGCAG 
CTGGTGGCCC GAGTGACATC TGTAGATCCT CTCTTGGACA AGCTGCATGG TCTGGTGCTG 
AGTGAAGATT CCTATGAAGT AGTGCGGTCT GAGACCACAA ACCAAGACAA GATGAGGAAG 
CTCTTCAGCC TCAGCCGGTC CTGGAGCTGG GATTGCAAAG ACCAATTCTA CCAAGCTCTG 
AAGGAGACCC ATCCTCACCT GGTCATGGAT ATACTTGAGA AGTTGGGTGG GGTCTCTGTG 
AAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169843.1
CDS	481..4029
Translation	MEESQSKQESSTKVAQHEGQEDVDPTFKTKKLMEVELMKHRVQLERNLKLRTFPGARTKQVKEALYPLLTWSSKSKNLFQNFTKLLLFKKLCQRGSENLVRESWYPCVPEEEAHMIDIQDLFGPNLGTQKKPQLVIIEGAAGIGKSTLARLVKRAWKEGKLYRNDFHHVFFFSCRELAQYEQLSLAELIVQGQEVPTAPIRQILSHPEKLLFILDGIDEPAWVLADQNPELCLHWSQTQPVHTLLGSLLGKSILPGASFLLTTRTTALQKFIPSLEQPCQVEVLGFTLFERKNYFYKYFGKKKGGVTTFTLVKSNSALLTLCEVPWVCWLVCTCLKKQMEQGGELSLTSQTTTALCLKYLSLTIPGQHMRTQLRDLCSLAAEGVCQRRTLFSESDLCKQGLDEHAIASFLKIGVLQKQASSLSYSFAHLCLQEFFAAMSYILDDSEERHADMKNDRIVETLVERYGRQNLFEAPTVRFLFGLLSKEELKKIEKLFSCSLHGKTKLKLLWHILGKSQPHQPPCLGLLHCLYENQDMELLTHVMHDLQGTIVPGPDDLAHTVLQTNVKHLVIQTDMDLMVVTFCIKFCCHVRSLQLNRKVQQGHKFTAPGMVLYRWTPITDASWKIFFSNLKLARNLEELDLSGNPLSYYAVHSLCTTLRKRGCQLKTLWLVECGLTSTYCSLLASVLSARSSLTELDLQLNDLGDGGVKMLCEGLRNPACNLSILWLDQASLSDQVIAELRTLEAKNPKLLISSTWKPHVMVPTMNMDKEEVGDSQALLKQQRQQSGDKHMEPLGTEDEFWGPTGPVTTEVVDRERNLYRVQLPMAGSYHCPSTGLHFVVTRAVTIEIEFCAWSQYLDKTPLQQSHMVVGPLFDIKAEQGAVTAVYLPHFVALQEGIVDSSLFHVAHFQEHGMVLETPARVEQHYAVLENPSFSPMGILLRMIPAVGHFIPITSTTLIYYHLYLEDVTFHLYLVPNDCSIRKAIDDEEMKFQFVRINKPPPVDALYLGSRYIVSSSKLVEIIPKELELCYRSPGESQLFSEIDIGHMDSEIKLQIKDKRHMNLKWEALLKPGDLRPALPKIATAPKDAPSLLHFMDQHREQLVARVTSVDPLLDKLHGLVLSEDSYEVVRSETTNQDKMRKLFSLSRSWSWDCKDQFYQALKETHPHLVMDILEKLGGVSVKS

Target ORF information:

RefSeq Version	XM_017314354.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, pyrin domain containing 1A (Nlrp1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314354.2

ORF Insert Sequence:

ATGGAAGAAT CTCAGTCCAA GCAGGAAAGT AGCACAAAAG TGGCTCAGCA TGAAGGTCAA 
GAGGATGTGG ACCCAACCTT CAAAACAAAG AAACTTATGG AGGTGGAGCT AATGAAGCAC 
AGAGTGCAGC TAGAGAGGAA CTTGAAGCTA AGAACCTTTC CAGGAGCACG TACGAAGCAA 
GTAAAAGAGG CTCTTTACCC TCTTCTAACA TGGTCATCAA AAAGCAAGAA TCTGTTCCAA 
AATTTCACAA AACTACTACT CTTTAAAAAA TTGTGCCAAA GAGGCTCAGA AAACTTGGTC 
AGGGAAAGCT GGTATCCATG TGTACCAGAA GAAGAAGCAC ATATGATTGA CATCCAAGAT 
TTATTTGGCC CAAACTTAGG TACTCAGAAA AAGCCTCAAT TAGTCATAAT AGAAGGAGCT 
GCTGGGATTG GGAAGTCAAC ACTGGCCAGA CTTGTGAAGA GAGCCTGGAA GGAAGGCAAG 
CTTTACAGGA ATGACTTCCA TCATGTCTTC TTCTTCAGCT GCAGAGAGCT GGCCCAGTAT 
GAGCAGCTGA GTCTGGCTGA GCTCATAGTA CAAGGCCAGG AAGTGCCCAC AGCTCCCATT 
AGGCAGATCC TGTCTCACCC TGAGAAGTTG CTCTTCATTC TGGATGGCAT AGATGAGCCA 
GCATGGGTCT TAGCAGACCA GAATCCTGAG TTATGTCTTC ACTGGAGTCA AACCCAGCCT 
GTGCACACTC TGCTGGGCAG TTTACTTGGG AAATCCATCC TTCCTGGGGC TTCCTTCTTG 
CTCACAACTC GCACCACAGC TCTACAGAAA TTCATTCCTT CCCTGGAGCA GCCATGTCAA 
GTGGAAGTCC TAGGATTCAC TTTGTTTGAA CGGAAAAACT ATTTCTACAA ATATTTTGGG 
AAGAAAAAAG GTGGAGTTAC TACTTTTACA TTAGTTAAAT CAAACTCAGC GCTCCTGACT 
CTGTGTGAAG TTCCCTGGGT GTGCTGGTTG GTCTGCACTT GCCTGAAAAA GCAGATGGAG 
CAGGGTGGAG AGCTCTCACT GACCTCACAG ACCACCACAG CACTCTGCCT GAAATACCTT 
TCCCTGACGA TCCCAGGGCA GCACATGAGG ACCCAGCTCA GAGACCTCTG CTCACTGGCT 
GCTGAGGGGG TCTGCCAAAG AAGGACCCTG TTCAGTGAAA GCGATCTCTG TAAGCAAGGG 
TTAGATGAGC ATGCCATTGC CAGTTTCCTG AAGATTGGTG TCCTTCAAAA GCAGGCCAGC 
TCTCTGAGCT ACAGCTTTGC CCACTTGTGT CTTCAGGAGT TCTTTGCAGC AATGTCCTAC 
ATCTTGGATG ATAGTGAGGA AAGACATGCT GATATGAAAA ATGACAGAAT TGTGGAAACA 
CTGGTAGAAA GGTATGGAAG ACAAAACCTG TTTGAGGCAC CCACTGTGCG TTTTTTATTT 
GGTCTTTTGA GTAAAGAGGA ATTGAAAAAA ATAGAAAAGC TCTTTTCCTG CAGCCTTCAT 
GGAAAGACAA AGTTGAAACT ACTATGGCAC ATCCTAGGGA AATCCCAACC CCATCAACCA 
CCTTGCCTGG GTTTGCTCCA CTGTCTGTAT GAAAATCAGG ATATGGAGCT CCTGACACAC 
GTGATGCATG ATCTGCAAGG AACAATAGTG CCTGGACCAG ATGATTTGGC ACACACAGTG 
TTGCAGACAA ACGTGAAGCA CCTGGTGATA CAGACAGACA TGGACCTCAT GGTGGTTACT 
TTCTGCATTA AGTTCTGCTG TCACGTGAGG AGTCTTCAGC TGAACAGGAA GGTACAGCAG 
GGACATAAGT TTACGGCCCC TGGGATGGTT CTGTACAGAT GGACCCCAAT CACTGATGCC 
AGCTGGAAGA TTTTCTTCTC CAATCTTAAG TTAGCCAGAA ACCTGGAGGA GTTGGACCTA 
AGTGGAAATC CACTGAGCTA CTATGCAGTA CACAGTCTTT GTACGACCCT GAGAAAGCGT 
GGCTGCCAAC TAAAGACTCT GTGGCTTGTT GAATGCGGCC TCACATCCAC ATACTGCTCA 
CTTCTGGCCT CAGTACTCAG TGCCCGCTCC AGCCTGACTG AGCTAGACCT GCAGCTGAAT 
GACCTGGGTG ATGGTGGTGT GAAGATGTTG TGTGAGGGGC TCAGGAATCC TGCCTGCAAT 
CTCAGCATCC TGTGGCTGGA CCAGGCATCT CTCAGTGACC AAGTGATTGC AGAGCTCAGA 
ACTCTGGAGG CAAAGAATCC AAAGCTGCTC ATCTCCAGCA CATGGAAGCC ACATGTGATG 
GTCCCTACGA TGAACATGGA TAAAGAAGAA GTGGGTGATA GCCAGGCCCT ACTGAAGCAG 
CAGAGACAGC AGTCAGGAGA CAAACACATG GAACCTCTGG GGACTGAAGA TGAGTTCTGG 
GGCCCTACAG GACCTGTGAC TACTGAGGTG GTTGACAGAG AGAGGAACCT GTACCGAGTT 
CAGTTACCCA TGGCTGGTTC CTACCACTGT CCCAGTACAG GACTCCACTT TGTGGTGACA 
AGGGCAGTGA CAATCGAGAT TGAATTCTGT GCCTGGAGCC AATATCTCGA CAAGACTCCC 
TTGCAGCAGA GTCACATGGT GGTTGGACCT CTGTTTGACA TCAAGGCTGA GCAAGGAGCT 
GTGACTGCAG TGTACCTCCC TCATTTTGTG GCCCTCCAAG AGGGAATCGT GGACAGCTCC 
TTGTTCCATG TTGCCCACTT TCAAGAACAT GGGATGGTCC TAGAAACACC AGCAAGGGTG 
GAACAGCATT ACGCAGTACT GGAAAACCCA AGCTTCTCCC CAATGGGGAT TCTACTGAGA 
ATGATTCCGG CTGTCGGACA CTTCATCCCC ATCACTTCCA CCACACTGAT CTACTATCAC 
CTCTATCTTG AAGACGTCAC CTTTCACCTT TACCTGGTCC CCAATGACTG CAGTATTCGA 
AAGGCCATTG ATGATGAAGA AATGAAGTTT CAGTTTGTGC GCATAAACAA GCCACCCCCA 
GTAGACGCTC TTTATCTTGG TTCCCGCTAT ATCGTGTCTT CCTCCAAATT GGTGGAAATT 
ATCCCAAAGG AACTAGAGCT GTGCTACCGA AGCCCTGGGG AATCCCAGCT CTTCTCTGAG 
ATCGACATTG GGCACATGGA TTCAGAGATT AAGCTGCAAA TCAAAGACAA GAGGCACATG 
AATCTCAAAT GGGAGGCCTT GCTGAAACCA GGGGATCTCA GACCTGCACT GCCCAAGATT 
GCTACAGCCC CCAAAGATGC TCCTTCCTTG CTGCACTTCA TGGACCAGCA TCGGGAGCAG 
CTGGTGGCCC GAGTGACATC TGTAGATCCT CTCTTGGACA AGCTGCATGG TCTGGTGCTG 
AGTGAAGATT CCTATGAAGT AGTGCGGTCT GAGACCACAA ACCAAGACAA GATGAGGAAG 
CTCTTCAGCC TCAGCCGGTC CTGGAGCTGG GATTGCAAAG ACCAATTCTA CCAAGCTCTG 
AAGGAGACCC ATCCTCACCT GGTCATGGAT ATACTTGAGA AGTTGGGTGG GGTCTCTGTG 
AAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp1a ( XM_017314355.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169844.1 Mus musculus NLR family, pyrin domain containing 1A (Nlrp1a), transcript variant X2, mRNA.

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CloneID	OMu19634
Clone ID Related Accession (Same CDS sequence)	XM_017314354.1 , XM_017314354.2 , XM_017314355.1 , NM_001004142.2
Accession Version	XM_017314355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NACHT, LRR and PYD domains-containing protein 1a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT CTCAGTCCAA GCAGGAAAGT AGCACAAAAG TGGCTCAGCA TGAAGGTCAA 
GAGGATGTGG ACCCAACCTT CAAAACAAAG AAACTTATGG AGGTGGAGCT AATGAAGCAC 
AGAGTGCAGC TAGAGAGGAA CTTGAAGCTA AGAACCTTTC CAGGAGCACG TACGAAGCAA 
GTAAAAGAGG CTCTTTACCC TCTTCTAACA TGGTCATCAA AAAGCAAGAA TCTGTTCCAA 
AATTTCACAA AACTACTACT CTTTAAAAAA TTGTGCCAAA GAGGCTCAGA AAACTTGGTC 
AGGGAAAGCT GGTATCCATG TGTACCAGAA GAAGAAGCAC ATATGATTGA CATCCAAGAT 
TTATTTGGCC CAAACTTAGG TACTCAGAAA AAGCCTCAAT TAGTCATAAT AGAAGGAGCT 
GCTGGGATTG GGAAGTCAAC ACTGGCCAGA CTTGTGAAGA GAGCCTGGAA GGAAGGCAAG 
CTTTACAGGA ATGACTTCCA TCATGTCTTC TTCTTCAGCT GCAGAGAGCT GGCCCAGTAT 
GAGCAGCTGA GTCTGGCTGA GCTCATAGTA CAAGGCCAGG AAGTGCCCAC AGCTCCCATT 
AGGCAGATCC TGTCTCACCC TGAGAAGTTG CTCTTCATTC TGGATGGCAT AGATGAGCCA 
GCATGGGTCT TAGCAGACCA GAATCCTGAG TTATGTCTTC ACTGGAGTCA AACCCAGCCT 
GTGCACACTC TGCTGGGCAG TTTACTTGGG AAATCCATCC TTCCTGGGGC TTCCTTCTTG 
CTCACAACTC GCACCACAGC TCTACAGAAA TTCATTCCTT CCCTGGAGCA GCCATGTCAA 
GTGGAAGTCC TAGGATTCAC TTTGTTTGAA CGGAAAAACT ATTTCTACAA ATATTTTGGG 
AAGAAAAAAG GTGGAGTTAC TACTTTTACA TTAGTTAAAT CAAACTCAGC GCTCCTGACT 
CTGTGTGAAG TTCCCTGGGT GTGCTGGTTG GTCTGCACTT GCCTGAAAAA GCAGATGGAG 
CAGGGTGGAG AGCTCTCACT GACCTCACAG ACCACCACAG CACTCTGCCT GAAATACCTT 
TCCCTGACGA TCCCAGGGCA GCACATGAGG ACCCAGCTCA GAGACCTCTG CTCACTGGCT 
GCTGAGGGGG TCTGCCAAAG AAGGACCCTG TTCAGTGAAA GCGATCTCTG TAAGCAAGGG 
TTAGATGAGC ATGCCATTGC CAGTTTCCTG AAGATTGGTG TCCTTCAAAA GCAGGCCAGC 
TCTCTGAGCT ACAGCTTTGC CCACTTGTGT CTTCAGGAGT TCTTTGCAGC AATGTCCTAC 
ATCTTGGATG ATAGTGAGGA AAGACATGCT GATATGAAAA ATGACAGAAT TGTGGAAACA 
CTGGTAGAAA GGTATGGAAG ACAAAACCTG TTTGAGGCAC CCACTGTGCG TTTTTTATTT 
GGTCTTTTGA GTAAAGAGGA ATTGAAAAAA ATAGAAAAGC TCTTTTCCTG CAGCCTTCAT 
GGAAAGACAA AGTTGAAACT ACTATGGCAC ATCCTAGGGA AATCCCAACC CCATCAACCA 
CCTTGCCTGG GTTTGCTCCA CTGTCTGTAT GAAAATCAGG ATATGGAGCT CCTGACACAC 
GTGATGCATG ATCTGCAAGG AACAATAGTG CCTGGACCAG ATGATTTGGC ACACACAGTG 
TTGCAGACAA ACGTGAAGCA CCTGGTGATA CAGACAGACA TGGACCTCAT GGTGGTTACT 
TTCTGCATTA AGTTCTGCTG TCACGTGAGG AGTCTTCAGC TGAACAGGAA GGTACAGCAG 
GGACATAAGT TTACGGCCCC TGGGATGGTT CTGTACAGAT GGACCCCAAT CACTGATGCC 
AGCTGGAAGA TTTTCTTCTC CAATCTTAAG TTAGCCAGAA ACCTGGAGGA GTTGGACCTA 
AGTGGAAATC CACTGAGCTA CTATGCAGTA CACAGTCTTT GTACGACCCT GAGAAAGCGT 
GGCTGCCAAC TAAAGACTCT GTGGCTTGTT GAATGCGGCC TCACATCCAC ATACTGCTCA 
CTTCTGGCCT CAGTACTCAG TGCCCGCTCC AGCCTGACTG AGCTAGACCT GCAGCTGAAT 
GACCTGGGTG ATGGTGGTGT GAAGATGTTG TGTGAGGGGC TCAGGAATCC TGCCTGCAAT 
CTCAGCATCC TGTGGCTGGA CCAGGCATCT CTCAGTGACC AAGTGATTGC AGAGCTCAGA 
ACTCTGGAGG CAAAGAATCC AAAGCTGCTC ATCTCCAGCA CATGGAAGCC ACATGTGATG 
GTCCCTACGA TGAACATGGA TAAAGAAGAA GTGGGTGATA GCCAGGCCCT ACTGAAGCAG 
CAGAGACAGC AGTCAGGAGA CAAACACATG GAACCTCTGG GGACTGAAGA TGAGTTCTGG 
GGCCCTACAG GACCTGTGAC TACTGAGGTG GTTGACAGAG AGAGGAACCT GTACCGAGTT 
CAGTTACCCA TGGCTGGTTC CTACCACTGT CCCAGTACAG GACTCCACTT TGTGGTGACA 
AGGGCAGTGA CAATCGAGAT TGAATTCTGT GCCTGGAGCC AATATCTCGA CAAGACTCCC 
TTGCAGCAGA GTCACATGGT GGTTGGACCT CTGTTTGACA TCAAGGCTGA GCAAGGAGCT 
GTGACTGCAG TGTACCTCCC TCATTTTGTG GCCCTCCAAG AGGGAATCGT GGACAGCTCC 
TTGTTCCATG TTGCCCACTT TCAAGAACAT GGGATGGTCC TAGAAACACC AGCAAGGGTG 
GAACAGCATT ACGCAGTACT GGAAAACCCA AGCTTCTCCC CAATGGGGAT TCTACTGAGA 
ATGATTCCGG CTGTCGGACA CTTCATCCCC ATCACTTCCA CCACACTGAT CTACTATCAC 
CTCTATCTTG AAGACGTCAC CTTTCACCTT TACCTGGTCC CCAATGACTG CAGTATTCGA 
AAGGCCATTG ATGATGAAGA AATGAAGTTT CAGTTTGTGC GCATAAACAA GCCACCCCCA 
GTAGACGCTC TTTATCTTGG TTCCCGCTAT ATCGTGTCTT CCTCCAAATT GGTGGAAATT 
ATCCCAAAGG AACTAGAGCT GTGCTACCGA AGCCCTGGGG AATCCCAGCT CTTCTCTGAG 
ATCGACATTG GGCACATGGA TTCAGAGATT AAGCTGCAAA TCAAAGACAA GAGGCACATG 
AATCTCAAAT GGGAGGCCTT GCTGAAACCA GGGGATCTCA GACCTGCACT GCCCAAGATT 
GCTACAGCCC CCAAAGATGC TCCTTCCTTG CTGCACTTCA TGGACCAGCA TCGGGAGCAG 
CTGGTGGCCC GAGTGACATC TGTAGATCCT CTCTTGGACA AGCTGCATGG TCTGGTGCTG 
AGTGAAGATT CCTATGAAGT AGTGCGGTCT GAGACCACAA ACCAAGACAA GATGAGGAAG 
CTCTTCAGCC TCAGCCGGTC CTGGAGCTGG GATTGCAAAG ACCAATTCTA CCAAGCTCTG 
AAGGAGACCC ATCCTCACCT GGTCATGGAT ATACTTGAGA AGTTGGGTGG GGTCTCTGTG 
AAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169844.1
CDS	917..4465
Translation	MEESQSKQESSTKVAQHEGQEDVDPTFKTKKLMEVELMKHRVQLERNLKLRTFPGARTKQVKEALYPLLTWSSKSKNLFQNFTKLLLFKKLCQRGSENLVRESWYPCVPEEEAHMIDIQDLFGPNLGTQKKPQLVIIEGAAGIGKSTLARLVKRAWKEGKLYRNDFHHVFFFSCRELAQYEQLSLAELIVQGQEVPTAPIRQILSHPEKLLFILDGIDEPAWVLADQNPELCLHWSQTQPVHTLLGSLLGKSILPGASFLLTTRTTALQKFIPSLEQPCQVEVLGFTLFERKNYFYKYFGKKKGGVTTFTLVKSNSALLTLCEVPWVCWLVCTCLKKQMEQGGELSLTSQTTTALCLKYLSLTIPGQHMRTQLRDLCSLAAEGVCQRRTLFSESDLCKQGLDEHAIASFLKIGVLQKQASSLSYSFAHLCLQEFFAAMSYILDDSEERHADMKNDRIVETLVERYGRQNLFEAPTVRFLFGLLSKEELKKIEKLFSCSLHGKTKLKLLWHILGKSQPHQPPCLGLLHCLYENQDMELLTHVMHDLQGTIVPGPDDLAHTVLQTNVKHLVIQTDMDLMVVTFCIKFCCHVRSLQLNRKVQQGHKFTAPGMVLYRWTPITDASWKIFFSNLKLARNLEELDLSGNPLSYYAVHSLCTTLRKRGCQLKTLWLVECGLTSTYCSLLASVLSARSSLTELDLQLNDLGDGGVKMLCEGLRNPACNLSILWLDQASLSDQVIAELRTLEAKNPKLLISSTWKPHVMVPTMNMDKEEVGDSQALLKQQRQQSGDKHMEPLGTEDEFWGPTGPVTTEVVDRERNLYRVQLPMAGSYHCPSTGLHFVVTRAVTIEIEFCAWSQYLDKTPLQQSHMVVGPLFDIKAEQGAVTAVYLPHFVALQEGIVDSSLFHVAHFQEHGMVLETPARVEQHYAVLENPSFSPMGILLRMIPAVGHFIPITSTTLIYYHLYLEDVTFHLYLVPNDCSIRKAIDDEEMKFQFVRINKPPPVDALYLGSRYIVSSSKLVEIIPKELELCYRSPGESQLFSEIDIGHMDSEIKLQIKDKRHMNLKWEALLKPGDLRPALPKIATAPKDAPSLLHFMDQHREQLVARVTSVDPLLDKLHGLVLSEDSYEVVRSETTNQDKMRKLFSLSRSWSWDCKDQFYQALKETHPHLVMDILEKLGGVSVKS

Target ORF information:

RefSeq Version	XM_017314355.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, pyrin domain containing 1A (Nlrp1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314355.1

ORF Insert Sequence:

ATGGAAGAAT CTCAGTCCAA GCAGGAAAGT AGCACAAAAG TGGCTCAGCA TGAAGGTCAA 
GAGGATGTGG ACCCAACCTT CAAAACAAAG AAACTTATGG AGGTGGAGCT AATGAAGCAC 
AGAGTGCAGC TAGAGAGGAA CTTGAAGCTA AGAACCTTTC CAGGAGCACG TACGAAGCAA 
GTAAAAGAGG CTCTTTACCC TCTTCTAACA TGGTCATCAA AAAGCAAGAA TCTGTTCCAA 
AATTTCACAA AACTACTACT CTTTAAAAAA TTGTGCCAAA GAGGCTCAGA AAACTTGGTC 
AGGGAAAGCT GGTATCCATG TGTACCAGAA GAAGAAGCAC ATATGATTGA CATCCAAGAT 
TTATTTGGCC CAAACTTAGG TACTCAGAAA AAGCCTCAAT TAGTCATAAT AGAAGGAGCT 
GCTGGGATTG GGAAGTCAAC ACTGGCCAGA CTTGTGAAGA GAGCCTGGAA GGAAGGCAAG 
CTTTACAGGA ATGACTTCCA TCATGTCTTC TTCTTCAGCT GCAGAGAGCT GGCCCAGTAT 
GAGCAGCTGA GTCTGGCTGA GCTCATAGTA CAAGGCCAGG AAGTGCCCAC AGCTCCCATT 
AGGCAGATCC TGTCTCACCC TGAGAAGTTG CTCTTCATTC TGGATGGCAT AGATGAGCCA 
GCATGGGTCT TAGCAGACCA GAATCCTGAG TTATGTCTTC ACTGGAGTCA AACCCAGCCT 
GTGCACACTC TGCTGGGCAG TTTACTTGGG AAATCCATCC TTCCTGGGGC TTCCTTCTTG 
CTCACAACTC GCACCACAGC TCTACAGAAA TTCATTCCTT CCCTGGAGCA GCCATGTCAA 
GTGGAAGTCC TAGGATTCAC TTTGTTTGAA CGGAAAAACT ATTTCTACAA ATATTTTGGG 
AAGAAAAAAG GTGGAGTTAC TACTTTTACA TTAGTTAAAT CAAACTCAGC GCTCCTGACT 
CTGTGTGAAG TTCCCTGGGT GTGCTGGTTG GTCTGCACTT GCCTGAAAAA GCAGATGGAG 
CAGGGTGGAG AGCTCTCACT GACCTCACAG ACCACCACAG CACTCTGCCT GAAATACCTT 
TCCCTGACGA TCCCAGGGCA GCACATGAGG ACCCAGCTCA GAGACCTCTG CTCACTGGCT 
GCTGAGGGGG TCTGCCAAAG AAGGACCCTG TTCAGTGAAA GCGATCTCTG TAAGCAAGGG 
TTAGATGAGC ATGCCATTGC CAGTTTCCTG AAGATTGGTG TCCTTCAAAA GCAGGCCAGC 
TCTCTGAGCT ACAGCTTTGC CCACTTGTGT CTTCAGGAGT TCTTTGCAGC AATGTCCTAC 
ATCTTGGATG ATAGTGAGGA AAGACATGCT GATATGAAAA ATGACAGAAT TGTGGAAACA 
CTGGTAGAAA GGTATGGAAG ACAAAACCTG TTTGAGGCAC CCACTGTGCG TTTTTTATTT 
GGTCTTTTGA GTAAAGAGGA ATTGAAAAAA ATAGAAAAGC TCTTTTCCTG CAGCCTTCAT 
GGAAAGACAA AGTTGAAACT ACTATGGCAC ATCCTAGGGA AATCCCAACC CCATCAACCA 
CCTTGCCTGG GTTTGCTCCA CTGTCTGTAT GAAAATCAGG ATATGGAGCT CCTGACACAC 
GTGATGCATG ATCTGCAAGG AACAATAGTG CCTGGACCAG ATGATTTGGC ACACACAGTG 
TTGCAGACAA ACGTGAAGCA CCTGGTGATA CAGACAGACA TGGACCTCAT GGTGGTTACT 
TTCTGCATTA AGTTCTGCTG TCACGTGAGG AGTCTTCAGC TGAACAGGAA GGTACAGCAG 
GGACATAAGT TTACGGCCCC TGGGATGGTT CTGTACAGAT GGACCCCAAT CACTGATGCC 
AGCTGGAAGA TTTTCTTCTC CAATCTTAAG TTAGCCAGAA ACCTGGAGGA GTTGGACCTA 
AGTGGAAATC CACTGAGCTA CTATGCAGTA CACAGTCTTT GTACGACCCT GAGAAAGCGT 
GGCTGCCAAC TAAAGACTCT GTGGCTTGTT GAATGCGGCC TCACATCCAC ATACTGCTCA 
CTTCTGGCCT CAGTACTCAG TGCCCGCTCC AGCCTGACTG AGCTAGACCT GCAGCTGAAT 
GACCTGGGTG ATGGTGGTGT GAAGATGTTG TGTGAGGGGC TCAGGAATCC TGCCTGCAAT 
CTCAGCATCC TGTGGCTGGA CCAGGCATCT CTCAGTGACC AAGTGATTGC AGAGCTCAGA 
ACTCTGGAGG CAAAGAATCC AAAGCTGCTC ATCTCCAGCA CATGGAAGCC ACATGTGATG 
GTCCCTACGA TGAACATGGA TAAAGAAGAA GTGGGTGATA GCCAGGCCCT ACTGAAGCAG 
CAGAGACAGC AGTCAGGAGA CAAACACATG GAACCTCTGG GGACTGAAGA TGAGTTCTGG 
GGCCCTACAG GACCTGTGAC TACTGAGGTG GTTGACAGAG AGAGGAACCT GTACCGAGTT 
CAGTTACCCA TGGCTGGTTC CTACCACTGT CCCAGTACAG GACTCCACTT TGTGGTGACA 
AGGGCAGTGA CAATCGAGAT TGAATTCTGT GCCTGGAGCC AATATCTCGA CAAGACTCCC 
TTGCAGCAGA GTCACATGGT GGTTGGACCT CTGTTTGACA TCAAGGCTGA GCAAGGAGCT 
GTGACTGCAG TGTACCTCCC TCATTTTGTG GCCCTCCAAG AGGGAATCGT GGACAGCTCC 
TTGTTCCATG TTGCCCACTT TCAAGAACAT GGGATGGTCC TAGAAACACC AGCAAGGGTG 
GAACAGCATT ACGCAGTACT GGAAAACCCA AGCTTCTCCC CAATGGGGAT TCTACTGAGA 
ATGATTCCGG CTGTCGGACA CTTCATCCCC ATCACTTCCA CCACACTGAT CTACTATCAC 
CTCTATCTTG AAGACGTCAC CTTTCACCTT TACCTGGTCC CCAATGACTG CAGTATTCGA 
AAGGCCATTG ATGATGAAGA AATGAAGTTT CAGTTTGTGC GCATAAACAA GCCACCCCCA 
GTAGACGCTC TTTATCTTGG TTCCCGCTAT ATCGTGTCTT CCTCCAAATT GGTGGAAATT 
ATCCCAAAGG AACTAGAGCT GTGCTACCGA AGCCCTGGGG AATCCCAGCT CTTCTCTGAG 
ATCGACATTG GGCACATGGA TTCAGAGATT AAGCTGCAAA TCAAAGACAA GAGGCACATG 
AATCTCAAAT GGGAGGCCTT GCTGAAACCA GGGGATCTCA GACCTGCACT GCCCAAGATT 
GCTACAGCCC CCAAAGATGC TCCTTCCTTG CTGCACTTCA TGGACCAGCA TCGGGAGCAG 
CTGGTGGCCC GAGTGACATC TGTAGATCCT CTCTTGGACA AGCTGCATGG TCTGGTGCTG 
AGTGAAGATT CCTATGAAGT AGTGCGGTCT GAGACCACAA ACCAAGACAA GATGAGGAAG 
CTCTTCAGCC TCAGCCGGTC CTGGAGCTGG GATTGCAAAG ACCAATTCTA CCAAGCTCTG 
AAGGAGACCC ATCCTCACCT GGTCATGGAT ATACTTGAGA AGTTGGGTGG GGTCTCTGTG 
AAATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mechanisms of NLRP1-Mediated Autoinflammatory Disease in Humans and Mice.
Journal of molecular biology430(2)142-152(2018 Jan)
Yu CH,Moecking J,Geyer M,Masters SL

Curcumin ameliorates neuropathic pain by down-regulating spinal IL-1? via suppressing astroglial NALP1 inflammasome and JAK2-STAT3 signalling.
Scientific reports628956(2016 Jul)
Liu S,Li Q,Zhang MT,Mao-Ying QL,Hu LY,Wu GC,Mi WL,Wang YQ

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Nlrp1a cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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