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Sema7a cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Sema7a
Entrez Gene ID 20361
Full Name sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A
Synonyms 2900057C09Rik,CDw108,H-Sema-L,M-Sema-L,Semal
General protein information
Preferred Names

sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A

Names

semaphorin-7A
H-Sema K1
Semaphorin K1
sema K1
sema L
semaphorin-K1
semaphorin-L
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

9

9|9 B

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006510889.2 XP_006510952.1 semaphorin-7A isoform X1
XM_030244149.1 XP_030100009.1 semaphorin-7A isoform X2
NM_011352.3 NP_035482.1 semaphorin-7A precursor
NM_011352.2 NP_035482.1 semaphorin-7A precursor
XM_006510888.3 XP_006510951.1 semaphorin-7A isoform X1
XM_006510889.3 XP_006510952.1 semaphorin-7A isoform X1

Pathways from BioSystems


Homologies


Bos taurus (cattle) SEMA7A NP_001193114.1
Homo sapiens (human) SEMA7A NP_003603.1
Gallus gallus (chicken) SEMA7A NP_001186678.1
Danio rerio (zebrafish) sema7a NP_001108357.1
SEMA7A XP_001099042.1
Canis lupus familiaris (dog) SEMA7A XP_005638673.1
Rattus norvegicus (Norway rat) Sema7a NP_001101623.1
Pan troglodytes (chimpanzee) SEMA7A XP_523219.2
Mus musculus (house mouse) Sema7a NP_035482.1
Xenopus tropicalis (tropical clawed frog) sema7a XP_002937527.2

Gene Ontology


Bibliography

Related articles in PubMed

Vascular Semaphorin 7A Upregulation by Disturbed Flow Promotes Atherosclerosis Through Endothelial ?1 Integrin.
Hu S, Liu Y, You T, Heath J, Xu L, Zheng X, Wang A, Wang Y, Li F, Yang F, Cao Y, Zhang H, van Gils JM, van Zonneveld AJ, Jo H, Wu Q, Zhang Y, Tang C, Zhu L
Arteriosclerosis, thrombosis, and vascular biology(2017 Dec)

Semaphorin7A aggravates coxsackievirusB3-induced viral myocarditis by increasing ?1?1-integrin macrophages and subsequent enhanced inflammatory response.
Wu X, Meng Y, Wang C, Yue Y, Dong C, Xiong S
Journal of molecular and cellular cardiology11448-57(2017 Nov)

Suppression of tumor-derived Semaphorin?7A and genetic ablation of host-derived Semaphorin?7A impairs tumor progression in a murine model of advanced breast carcinoma.
Garcia-Areas R, Libreros S, Simoes M, Castro-Silva C, Gazaniga N, Amat S, Jaczewska J, Keating P, Schilling K, Brito M, Wojcikiewicz EP, Iragavarpu-Charyulu V
International journal of oncology51(5)1395-1404(2017 Nov)

Transcriptional repression of Plxnc1 by Lmx1a and Lmx1b directs topographic dopaminergic circuit formation.
Chabrat A, Brisson G, Doucet-Beaupr? H, Salesse C, Schaan Profes M, Dovonou A, Akitegetse C, Charest J, Lemstra S, C?t? D, Pasterkamp RJ, Abrudan MI, Metzakopian E, Ang SL, L?vesque M
Nature communications8(1)933(2017 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings indicate that Sema7A as a potent activator of T cells and monocytes in the immune response contributes to the inflammation and progression of rheumatoid arthritis
Title: Semaphorin 7A as a potential immune regulator and promising therapeutic target in rheumatoid arthritis.

Our results suggest that semaphorin 7a may be novel target for blocking breast tumor progression.
Title: Semaphorin 7a exerts pleiotropic effects to promote breast tumor progression.

Data suggest that sema7A is involved in the pathogenesis of experimental autoimmune encephalomyelitis, in the modulation of the immune response and in the neurodegeneration that take place in the CNS.
Title: Differential expression of sema3A and sema7A in a murine model of multiple sclerosis: Implications for a therapeutic design.

This study demonstrates that Sema7A controls the assembly of actin-based protrusions that drive Dendritic cell migration in response to CCL21.
Title: Semaphorin 7A Promotes Chemokine-Driven Dendritic Cell Migration.

SEMA7A has a role in the development of lung injury
Title: Semaphorin 7A Aggravates Pulmonary Inflammation during Lung Injury.

The following Sema7a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema7a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu14221 NM_011352.3
Latest version!		 Mus musculus sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $307.30
$439.00
OMu42610 XM_006510888.3
Latest version!		 Mus musculus sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $307.30
$439.00
OMu90110 XM_030244149.1
Latest version!		 Mus musculus sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OMu42610 XM_006510889.3
Latest version!		 Mus musculus sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OMu14221 NM_011352.2 Mus musculus sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $307.30
$439.00
OMu42610 XM_006510889.2 Mus musculus sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $307.30
$439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu14221
Clone ID Related Accession (Same CDS sequence) NM_011352.3 , NM_011352.2
Accession Version NM_011352.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1995bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-24
Organism Mus musculus(house mouse)
Product semaphorin-7A precursor
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC165246.2. On Jun 19, 2018 this sequence version replaced NM_011352.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057875.1, AK156123.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGACGCCTC CTCCTCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCTCAGC 
CTGCCGGCTC GGTTCGGGCT CCCGCTGCGG CTGCGGCTTC TGCTGGTGTT CTGGGTGGCC 
GCCGCCTCCG CCCAAGGCCA CTCGAGGAGC GGACCCCGCA TCTCCGCCGT CTGGAAAGGG 
CAGGACCATG TGGACTTTAG CCAGCCTGAG CCACACACCG TGCTTTTCCA TGAGCCGGGC 
AGCTTCTCTG TCTGGGTGGG TGGACGTGGC AAGGTCTACC ACTTCAACTT CCCCGAGGGC 
AAGAATGCCT CTGTGCGCAC GGTGAACATC GGCTCCACAA AGGGGTCCTG TCAGGACAAA 
CAGGACTGTG GGAATTACAT CACTCTTCTA GAAAGGCGGG GTAATGGGCT GCTGGTCTGT 
GGCACCAATG CCCGGAAGCC CAGCTGCTGG AACTTGGTGA ATGACAGTGT GGTGATGTCA 
CTTGGTGAGA TGAAAGGCTA TGCCCCCTTC AGCCCGGATG AGAACTCCCT GGTTCTGTTT 
GAAGGAGATG AAGTGTACTC TACCATCCGG AAGCAGGAAT ACAACGGGAA GATCCCTCGG 
TTTCGACGCA TTCGGGGCGA GAGTGAACTG TACACAAGTG ATACAGTCAT GCAGAACCCA 
CAGTTCATCA AGGCCACCAT TGTGCACCAA GACCAAGCCT ATGATGATAA GATCTACTAC 
TTCTTCCGAG AAGACAACCC TGACAAGAAC CCCGAGGCTC CTCTCAATGT GTCCCGAGTA 
GCCCAGTTGT GCAGGGGGGA CCAGGGTGGT GAGAGTTCGT TGTCTGTCTC CAAGTGGAAC 
ACCTTCCTGA AAGCCATGTT GGTCTGCAGC GATGCAGCCA CCAACAGGAA CTTCAATCGG 
CTGCAAGATG TCTTCCTGCT CCCTGACCCC AGTGGCCAGT GGAGAGATAC CAGGGTCTAT 
GGCGTTTTCT CCAACCCCTG GAACTACTCA GCTGTCTGCG TGTATTCGCT TGGTGACATT 
GACAGAGTCT TCCGTACCTC ATCGCTCAAA GGCTACCACA TGGGCCTTCC CAACCCTCGA 
CCTGGCATGT GCCTCCCAAA AAAGCAGCCC ATACCCACAG AAACCTTCCA GGTAGCTGAT 
AGTCACCCAG AGGTGGCTCA GAGGGTGGAA CCTATGGGGC CACTGAAGAC ACCATTGTTC 
CATTCTAAGT ACCATTACCA GAAAGTGGTC GTTCACCGCA TGCAAGCCAG CAATGGAGAG 
ACCTTCCATG TGCTTTATCT AACCACAGAC AGGGGCACCA TTCACAAGGT GGTGGAATCA 
GGGGACCAGG ACCATAGCTT TGTCTTCAAT ATCATGGAGA TCCAGCCCTT TCACCGTGCA 
GCGGCCATCC AGGCTATATC ATTGGATGCT GACCGGCGGA AGCTCTATGT GACCTCCCAG 
TGGGAAGTGA GCCAGGTACC CCTGGACATG TGTGAGGTCT ACAGCGGGGG CTGCCATGGC 
TGCCTCATGT CCCGAGACCC CTACTGTGGC TGGGACCAGG ACCGCTGCGT GTCTATCTAC 
AGCTCCCAAC GATCAGTGCT GCAGTCCATT AATCCAGCGG AGCCACACAG AGAGTGTCCC 
AACCCTAAAC CAGACGAGGC TCCATTGCAG AAGGTTTCCC TGGCTCGGAA CTCTCGATAC 
TACCTGACCT GCCCCATGGA GTCCCGCCAC GCCACTTACT TATGGCGCCA TGAGGAGAAT 
GTGGAACAGA GCTGTGAGCC AGGCCACCAA AGCCCTAGCT GCATCCTGTT CATTGAGAAC 
CTCACGGCCC GTCAGTATGG CCACTACCGC TGCGAGGCCC AAGAGGGCTC CTACCTCCGT 
GAGGCTCAAC ACTGGGAGCT GCTGCCAGAG GACAGAGCAC TGGCCGAACA ACTAATGGGC 
CATGCCCGGG CCCTGGCCGC CTCCTTCTGG CTGGGGGTCC TGCCCACACT CATACTTGGT 
CTGCTGGTTC ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_035482.1
CDS40..2034
Translation

Target ORF information:

RefSeq Version NM_011352.3
Organism Mus musculus(house mouse)
Definition Mus musculus sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_011352.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGACGCCTC CTCCTCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCTCAGC 
CTGCCGGCTC GGTTCGGGCT CCCGCTGCGG CTGCGGCTTC TGCTGGTGTT CTGGGTGGCC 
GCCGCCTCCG CCCAAGGCCA CTCGAGGAGC GGACCCCGCA TCTCCGCCGT CTGGAAAGGG 
CAGGACCATG TGGACTTTAG CCAGCCTGAG CCACACACCG TGCTTTTCCA TGAGCCGGGC 
AGCTTCTCTG TCTGGGTGGG TGGACGTGGC AAGGTCTACC ACTTCAACTT CCCCGAGGGC 
AAGAATGCCT CTGTGCGCAC GGTGAACATC GGCTCCACAA AGGGGTCCTG TCAGGACAAA 
CAGGACTGTG GGAATTACAT CACTCTTCTA GAAAGGCGGG GTAATGGGCT GCTGGTCTGT 
GGCACCAATG CCCGGAAGCC CAGCTGCTGG AACTTGGTGA ATGACAGTGT GGTGATGTCA 
CTTGGTGAGA TGAAAGGCTA TGCCCCCTTC AGCCCGGATG AGAACTCCCT GGTTCTGTTT 
GAAGGAGATG AAGTGTACTC TACCATCCGG AAGCAGGAAT ACAACGGGAA GATCCCTCGG 
TTTCGACGCA TTCGGGGCGA GAGTGAACTG TACACAAGTG ATACAGTCAT GCAGAACCCA 
CAGTTCATCA AGGCCACCAT TGTGCACCAA GACCAAGCCT ATGATGATAA GATCTACTAC 
TTCTTCCGAG AAGACAACCC TGACAAGAAC CCCGAGGCTC CTCTCAATGT GTCCCGAGTA 
GCCCAGTTGT GCAGGGGGGA CCAGGGTGGT GAGAGTTCGT TGTCTGTCTC CAAGTGGAAC 
ACCTTCCTGA AAGCCATGTT GGTCTGCAGC GATGCAGCCA CCAACAGGAA CTTCAATCGG 
CTGCAAGATG TCTTCCTGCT CCCTGACCCC AGTGGCCAGT GGAGAGATAC CAGGGTCTAT 
GGCGTTTTCT CCAACCCCTG GAACTACTCA GCTGTCTGCG TGTATTCGCT TGGTGACATT 
GACAGAGTCT TCCGTACCTC ATCGCTCAAA GGCTACCACA TGGGCCTTCC CAACCCTCGA 
CCTGGCATGT GCCTCCCAAA AAAGCAGCCC ATACCCACAG AAACCTTCCA GGTAGCTGAT 
AGTCACCCAG AGGTGGCTCA GAGGGTGGAA CCTATGGGGC CACTGAAGAC ACCATTGTTC 
CATTCTAAGT ACCATTACCA GAAAGTGGTC GTTCACCGCA TGCAAGCCAG CAATGGAGAG 
ACCTTCCATG TGCTTTATCT AACCACAGAC AGGGGCACCA TTCACAAGGT GGTGGAATCA 
GGGGACCAGG ACCATAGCTT TGTCTTCAAT ATCATGGAGA TCCAGCCCTT TCACCGTGCA 
GCGGCCATCC AGGCTATATC ATTGGATGCT GACCGGCGGA AGCTCTATGT GACCTCCCAG 
TGGGAAGTGA GCCAGGTACC CCTGGACATG TGTGAGGTCT ACAGCGGGGG CTGCCATGGC 
TGCCTCATGT CCCGAGACCC CTACTGTGGC TGGGACCAGG ACCGCTGCGT GTCTATCTAC 
AGCTCCCAAC GATCAGTGCT GCAGTCCATT AATCCAGCGG AGCCACACAG AGAGTGTCCC 
AACCCTAAAC CAGACGAGGC TCCATTGCAG AAGGTTTCCC TGGCTCGGAA CTCTCGATAC 
TACCTGACCT GCCCCATGGA GTCCCGCCAC GCCACTTACT TATGGCGCCA TGAGGAGAAT 
GTGGAACAGA GCTGTGAGCC AGGCCACCAA AGCCCTAGCT GCATCCTGTT CATTGAGAAC 
CTCACGGCCC GTCAGTATGG CCACTACCGC TGCGAGGCCC AAGAGGGCTC CTACCTCCGT 
GAGGCTCAAC ACTGGGAGCT GCTGCCAGAG GACAGAGCAC TGGCCGAACA ACTAATGGGC 
CATGCCCGGG CCCTGGCCGC CTCCTTCTGG CTGGGGGTCC TGCCCACACT CATACTTGGT 
CTGCTGGTTC ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu14221
Clone ID Related Accession (Same CDS sequence) NM_011352.3 , NM_011352.2
Accession Version NM_011352.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1995bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-16
Organism Mus musculus(house mouse)
Product semaphorin-7A precursor
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC057875.1. On Dec 20, 2003 this sequence version replaced NM_011352.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057875.1, AK156123.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGACGCCTC CTCCTCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCTCAGC 
CTGCCGGCTC GGTTCGGGCT CCCGCTGCGG CTGCGGCTTC TGCTGGTGTT CTGGGTGGCC 
GCCGCCTCCG CCCAAGGCCA CTCGAGGAGC GGACCCCGCA TCTCCGCCGT CTGGAAAGGG 
CAGGACCATG TGGACTTTAG CCAGCCTGAG CCACACACCG TGCTTTTCCA TGAGCCGGGC 
AGCTTCTCTG TCTGGGTGGG TGGACGTGGC AAGGTCTACC ACTTCAACTT CCCCGAGGGC 
AAGAATGCCT CTGTGCGCAC GGTGAACATC GGCTCCACAA AGGGGTCCTG TCAGGACAAA 
CAGGACTGTG GGAATTACAT CACTCTTCTA GAAAGGCGGG GTAATGGGCT GCTGGTCTGT 
GGCACCAATG CCCGGAAGCC CAGCTGCTGG AACTTGGTGA ATGACAGTGT GGTGATGTCA 
CTTGGTGAGA TGAAAGGCTA TGCCCCCTTC AGCCCGGATG AGAACTCCCT GGTTCTGTTT 
GAAGGAGATG AAGTGTACTC TACCATCCGG AAGCAGGAAT ACAACGGGAA GATCCCTCGG 
TTTCGACGCA TTCGGGGCGA GAGTGAACTG TACACAAGTG ATACAGTCAT GCAGAACCCA 
CAGTTCATCA AGGCCACCAT TGTGCACCAA GACCAAGCCT ATGATGATAA GATCTACTAC 
TTCTTCCGAG AAGACAACCC TGACAAGAAC CCCGAGGCTC CTCTCAATGT GTCCCGAGTA 
GCCCAGTTGT GCAGGGGGGA CCAGGGTGGT GAGAGTTCGT TGTCTGTCTC CAAGTGGAAC 
ACCTTCCTGA AAGCCATGTT GGTCTGCAGC GATGCAGCCA CCAACAGGAA CTTCAATCGG 
CTGCAAGATG TCTTCCTGCT CCCTGACCCC AGTGGCCAGT GGAGAGATAC CAGGGTCTAT 
GGCGTTTTCT CCAACCCCTG GAACTACTCA GCTGTCTGCG TGTATTCGCT TGGTGACATT 
GACAGAGTCT TCCGTACCTC ATCGCTCAAA GGCTACCACA TGGGCCTTCC CAACCCTCGA 
CCTGGCATGT GCCTCCCAAA AAAGCAGCCC ATACCCACAG AAACCTTCCA GGTAGCTGAT 
AGTCACCCAG AGGTGGCTCA GAGGGTGGAA CCTATGGGGC CACTGAAGAC ACCATTGTTC 
CATTCTAAGT ACCATTACCA GAAAGTGGTC GTTCACCGCA TGCAAGCCAG CAATGGAGAG 
ACCTTCCATG TGCTTTATCT AACCACAGAC AGGGGCACCA TTCACAAGGT GGTGGAATCA 
GGGGACCAGG ACCATAGCTT TGTCTTCAAT ATCATGGAGA TCCAGCCCTT TCACCGTGCA 
GCGGCCATCC AGGCTATATC ATTGGATGCT GACCGGCGGA AGCTCTATGT GACCTCCCAG 
TGGGAAGTGA GCCAGGTACC CCTGGACATG TGTGAGGTCT ACAGCGGGGG CTGCCATGGC 
TGCCTCATGT CCCGAGACCC CTACTGTGGC TGGGACCAGG ACCGCTGCGT GTCTATCTAC 
AGCTCCCAAC GATCAGTGCT GCAGTCCATT AATCCAGCGG AGCCACACAG AGAGTGTCCC 
AACCCTAAAC CAGACGAGGC TCCATTGCAG AAGGTTTCCC TGGCTCGGAA CTCTCGATAC 
TACCTGACCT GCCCCATGGA GTCCCGCCAC GCCACTTACT TATGGCGCCA TGAGGAGAAT 
GTGGAACAGA GCTGTGAGCC AGGCCACCAA AGCCCTAGCT GCATCCTGTT CATTGAGAAC 
CTCACGGCCC GTCAGTATGG CCACTACCGC TGCGAGGCCC AAGAGGGCTC CTACCTCCGT 
GAGGCTCAAC ACTGGGAGCT GCTGCCAGAG GACAGAGCAC TGGCCGAACA ACTAATGGGC 
CATGCCCGGG CCCTGGCCGC CTCCTTCTGG CTGGGGGTCC TGCCCACACT CATACTTGGT 
CTGCTGGTTC ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_035482.1
CDS17..2011
Translation

Target ORF information:

RefSeq Version NM_011352.2
Organism Mus musculus(house mouse)
Definition Mus musculus sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_011352.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGACGCCTC CTCCTCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCTCAGC 
CTGCCGGCTC GGTTCGGGCT CCCGCTGCGG CTGCGGCTTC TGCTGGTGTT CTGGGTGGCC 
GCCGCCTCCG CCCAAGGCCA CTCGAGGAGC GGACCCCGCA TCTCCGCCGT CTGGAAAGGG 
CAGGACCATG TGGACTTTAG CCAGCCTGAG CCACACACCG TGCTTTTCCA TGAGCCGGGC 
AGCTTCTCTG TCTGGGTGGG TGGACGTGGC AAGGTCTACC ACTTCAACTT CCCCGAGGGC 
AAGAATGCCT CTGTGCGCAC GGTGAACATC GGCTCCACAA AGGGGTCCTG TCAGGACAAA 
CAGGACTGTG GGAATTACAT CACTCTTCTA GAAAGGCGGG GTAATGGGCT GCTGGTCTGT 
GGCACCAATG CCCGGAAGCC CAGCTGCTGG AACTTGGTGA ATGACAGTGT GGTGATGTCA 
CTTGGTGAGA TGAAAGGCTA TGCCCCCTTC AGCCCGGATG AGAACTCCCT GGTTCTGTTT 
GAAGGAGATG AAGTGTACTC TACCATCCGG AAGCAGGAAT ACAACGGGAA GATCCCTCGG 
TTTCGACGCA TTCGGGGCGA GAGTGAACTG TACACAAGTG ATACAGTCAT GCAGAACCCA 
CAGTTCATCA AGGCCACCAT TGTGCACCAA GACCAAGCCT ATGATGATAA GATCTACTAC 
TTCTTCCGAG AAGACAACCC TGACAAGAAC CCCGAGGCTC CTCTCAATGT GTCCCGAGTA 
GCCCAGTTGT GCAGGGGGGA CCAGGGTGGT GAGAGTTCGT TGTCTGTCTC CAAGTGGAAC 
ACCTTCCTGA AAGCCATGTT GGTCTGCAGC GATGCAGCCA CCAACAGGAA CTTCAATCGG 
CTGCAAGATG TCTTCCTGCT CCCTGACCCC AGTGGCCAGT GGAGAGATAC CAGGGTCTAT 
GGCGTTTTCT CCAACCCCTG GAACTACTCA GCTGTCTGCG TGTATTCGCT TGGTGACATT 
GACAGAGTCT TCCGTACCTC ATCGCTCAAA GGCTACCACA TGGGCCTTCC CAACCCTCGA 
CCTGGCATGT GCCTCCCAAA AAAGCAGCCC ATACCCACAG AAACCTTCCA GGTAGCTGAT 
AGTCACCCAG AGGTGGCTCA GAGGGTGGAA CCTATGGGGC CACTGAAGAC ACCATTGTTC 
CATTCTAAGT ACCATTACCA GAAAGTGGTC GTTCACCGCA TGCAAGCCAG CAATGGAGAG 
ACCTTCCATG TGCTTTATCT AACCACAGAC AGGGGCACCA TTCACAAGGT GGTGGAATCA 
GGGGACCAGG ACCATAGCTT TGTCTTCAAT ATCATGGAGA TCCAGCCCTT TCACCGTGCA 
GCGGCCATCC AGGCTATATC ATTGGATGCT GACCGGCGGA AGCTCTATGT GACCTCCCAG 
TGGGAAGTGA GCCAGGTACC CCTGGACATG TGTGAGGTCT ACAGCGGGGG CTGCCATGGC 
TGCCTCATGT CCCGAGACCC CTACTGTGGC TGGGACCAGG ACCGCTGCGT GTCTATCTAC 
AGCTCCCAAC GATCAGTGCT GCAGTCCATT AATCCAGCGG AGCCACACAG AGAGTGTCCC 
AACCCTAAAC CAGACGAGGC TCCATTGCAG AAGGTTTCCC TGGCTCGGAA CTCTCGATAC 
TACCTGACCT GCCCCATGGA GTCCCGCCAC GCCACTTACT TATGGCGCCA TGAGGAGAAT 
GTGGAACAGA GCTGTGAGCC AGGCCACCAA AGCCCTAGCT GCATCCTGTT CATTGAGAAC 
CTCACGGCCC GTCAGTATGG CCACTACCGC TGCGAGGCCC AAGAGGGCTC CTACCTCCGT 
GAGGCTCAAC ACTGGGAGCT GCTGCCAGAG GACAGAGCAC TGGCCGAACA ACTAATGGGC 
CATGCCCGGG CCCTGGCCGC CTCCTTCTGG CTGGGGGTCC TGCCCACACT CATACTTGGT 
CTGCTGGTTC ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42610
Clone ID Related Accession (Same CDS sequence) XM_006510889.2 , XM_006510888.3 , XM_006510889.3
Accession Version XM_006510889.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1521bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product semaphorin-7A isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCACTTG GTGAGATGAA AGGCTATGCC CCCTTCAGCC CGGATGAGAA CTCCCTGGTT 
CTGTTTGAAG GAGATGAAGT GTACTCTACC ATCCGGAAGC AGGAATACAA CGGGAAGATC 
CCTCGGTTTC GACGCATTCG GGGCGAGAGT GAACTGTACA CAAGTGATAC AGTCATGCAG 
AACCCACAGT TCATCAAGGC CACCATTGTG CACCAAGACC AAGCCTATGA TGATAAGATC 
TACTACTTCT TCCGAGAAGA CAACCCTGAC AAGAACCCCG AGGCTCCTCT CAATGTGTCC 
CGAGTAGCCC AGTTGTGCAG GGGGGACCAG GGTGGTGAGA GTTCGTTGTC TGTCTCCAAG 
TGGAACACCT TCCTGAAAGC CATGTTGGTC TGCAGCGATG CAGCCACCAA CAGGAACTTC 
AATCGGCTGC AAGATGTCTT CCTGCTCCCT GACCCCAGTG GCCAGTGGAG AGATACCAGG 
GTCTATGGCG TTTTCTCCAA CCCCTGGAAC TACTCAGCTG TCTGCGTGTA TTCGCTTGGT 
GACATTGACA GAGTCTTCCG TACCTCATCG CTCAAAGGCT ACCACATGGG CCTTCCCAAC 
CCTCGACCTG GCATGTGCCT CCCAAAAAAG CAGCCCATAC CCACAGAAAC CTTCCAGGTA 
GCTGATAGTC ACCCAGAGGT GGCTCAGAGG GTGGAACCTA TGGGGCCACT GAAGACACCA 
TTGTTCCATT CTAAGTACCA TTACCAGAAA GTGGTCGTTC ACCGCATGCA AGCCAGCAAT 
GGAGAGACCT TCCATGTGCT TTATCTAACC ACAGACAGGG GCACCATTCA CAAGGTGGTG 
GAATCAGGGG ACCAGGACCA TAGCTTTGTC TTCAATATCA TGGAGATCCA GCCCTTTCAC 
CGTGCAGCGG CCATCCAGGC TATATCATTG GATGCTGACC GGCGGAAGCT CTATGTGACC 
TCCCAGTGGG AAGTGAGCCA GGTACCCCTG GACATGTGTG AGGTCTACAG CGGGGGCTGC 
CATGGCTGCC TCATGTCCCG AGACCCCTAC TGTGGCTGGG ACCAGGACCG CTGCGTGTCT 
ATCTACAGCT CCCAACGATC AGTGCTGCAG TCCATTAATC CAGCGGAGCC ACACAGAGAG 
TGTCCCAACC CTAAACCAGA CGAGGCTCCA TTGCAGAAGG TTTCCCTGGC TCGGAACTCT 
CGATACTACC TGACCTGCCC CATGGAGTCC CGCCACGCCA CTTACTTATG GCGCCATGAG 
GAGAATGTGG AACAGAGCTG TGAGCCAGGC CACCAAAGCC CTAGCTGCAT CCTGTTCATT 
GAGAACCTCA CGGCCCGTCA GTATGGCCAC TACCGCTGCG AGGCCCAAGA GGGCTCCTAC 
CTCCGTGAGG CTCAACACTG GGAGCTGCTG CCAGAGGACA GAGCACTGGC CGAACAACTA 
ATGGGCCATG CCCGGGCCCT GGCCGCCTCC TTCTGGCTGG GGGTCCTGCC CACACTCATA 
CTTGGTCTGC TGGTTCACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006510952.1
CDS325..1845
Translation

Target ORF information:

RefSeq Version XM_006510889.2
Organism Mus musculus(house mouse)
Definition Mus musculus sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006510889.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCACTTG GTGAGATGAA AGGCTATGCC CCCTTCAGCC CGGATGAGAA CTCCCTGGTT 
CTGTTTGAAG GAGATGAAGT GTACTCTACC ATCCGGAAGC AGGAATACAA CGGGAAGATC 
CCTCGGTTTC GACGCATTCG GGGCGAGAGT GAACTGTACA CAAGTGATAC AGTCATGCAG 
AACCCACAGT TCATCAAGGC CACCATTGTG CACCAAGACC AAGCCTATGA TGATAAGATC 
TACTACTTCT TCCGAGAAGA CAACCCTGAC AAGAACCCCG AGGCTCCTCT CAATGTGTCC 
CGAGTAGCCC AGTTGTGCAG GGGGGACCAG GGTGGTGAGA GTTCGTTGTC TGTCTCCAAG 
TGGAACACCT TCCTGAAAGC CATGTTGGTC TGCAGCGATG CAGCCACCAA CAGGAACTTC 
AATCGGCTGC AAGATGTCTT CCTGCTCCCT GACCCCAGTG GCCAGTGGAG AGATACCAGG 
GTCTATGGCG TTTTCTCCAA CCCCTGGAAC TACTCAGCTG TCTGCGTGTA TTCGCTTGGT 
GACATTGACA GAGTCTTCCG TACCTCATCG CTCAAAGGCT ACCACATGGG CCTTCCCAAC 
CCTCGACCTG GCATGTGCCT CCCAAAAAAG CAGCCCATAC CCACAGAAAC CTTCCAGGTA 
GCTGATAGTC ACCCAGAGGT GGCTCAGAGG GTGGAACCTA TGGGGCCACT GAAGACACCA 
TTGTTCCATT CTAAGTACCA TTACCAGAAA GTGGTCGTTC ACCGCATGCA AGCCAGCAAT 
GGAGAGACCT TCCATGTGCT TTATCTAACC ACAGACAGGG GCACCATTCA CAAGGTGGTG 
GAATCAGGGG ACCAGGACCA TAGCTTTGTC TTCAATATCA TGGAGATCCA GCCCTTTCAC 
CGTGCAGCGG CCATCCAGGC TATATCATTG GATGCTGACC GGCGGAAGCT CTATGTGACC 
TCCCAGTGGG AAGTGAGCCA GGTACCCCTG GACATGTGTG AGGTCTACAG CGGGGGCTGC 
CATGGCTGCC TCATGTCCCG AGACCCCTAC TGTGGCTGGG ACCAGGACCG CTGCGTGTCT 
ATCTACAGCT CCCAACGATC AGTGCTGCAG TCCATTAATC CAGCGGAGCC ACACAGAGAG 
TGTCCCAACC CTAAACCAGA CGAGGCTCCA TTGCAGAAGG TTTCCCTGGC TCGGAACTCT 
CGATACTACC TGACCTGCCC CATGGAGTCC CGCCACGCCA CTTACTTATG GCGCCATGAG 
GAGAATGTGG AACAGAGCTG TGAGCCAGGC CACCAAAGCC CTAGCTGCAT CCTGTTCATT 
GAGAACCTCA CGGCCCGTCA GTATGGCCAC TACCGCTGCG AGGCCCAAGA GGGCTCCTAC 
CTCCGTGAGG CTCAACACTG GGAGCTGCTG CCAGAGGACA GAGCACTGGC CGAACAACTA 
ATGGGCCATG CCCGGGCCCT GGCCGCCTCC TTCTGGCTGG GGGTCCTGCC CACACTCATA 
CTTGGTCTGC TGGTTCACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42610
Clone ID Related Accession (Same CDS sequence) XM_006510889.2 , XM_006510888.3 , XM_006510889.3
Accession Version XM_006510888.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1521bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product semaphorin-7A isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510888.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCACTTG GTGAGATGAA AGGCTATGCC CCCTTCAGCC CGGATGAGAA CTCCCTGGTT 
CTGTTTGAAG GAGATGAAGT GTACTCTACC ATCCGGAAGC AGGAATACAA CGGGAAGATC 
CCTCGGTTTC GACGCATTCG GGGCGAGAGT GAACTGTACA CAAGTGATAC AGTCATGCAG 
AACCCACAGT TCATCAAGGC CACCATTGTG CACCAAGACC AAGCCTATGA TGATAAGATC 
TACTACTTCT TCCGAGAAGA CAACCCTGAC AAGAACCCCG AGGCTCCTCT CAATGTGTCC 
CGAGTAGCCC AGTTGTGCAG GGGGGACCAG GGTGGTGAGA GTTCGTTGTC TGTCTCCAAG 
TGGAACACCT TCCTGAAAGC CATGTTGGTC TGCAGCGATG CAGCCACCAA CAGGAACTTC 
AATCGGCTGC AAGATGTCTT CCTGCTCCCT GACCCCAGTG GCCAGTGGAG AGATACCAGG 
GTCTATGGCG TTTTCTCCAA CCCCTGGAAC TACTCAGCTG TCTGCGTGTA TTCGCTTGGT 
GACATTGACA GAGTCTTCCG TACCTCATCG CTCAAAGGCT ACCACATGGG CCTTCCCAAC 
CCTCGACCTG GCATGTGCCT CCCAAAAAAG CAGCCCATAC CCACAGAAAC CTTCCAGGTA 
GCTGATAGTC ACCCAGAGGT GGCTCAGAGG GTGGAACCTA TGGGGCCACT GAAGACACCA 
TTGTTCCATT CTAAGTACCA TTACCAGAAA GTGGTCGTTC ACCGCATGCA AGCCAGCAAT 
GGAGAGACCT TCCATGTGCT TTATCTAACC ACAGACAGGG GCACCATTCA CAAGGTGGTG 
GAATCAGGGG ACCAGGACCA TAGCTTTGTC TTCAATATCA TGGAGATCCA GCCCTTTCAC 
CGTGCAGCGG CCATCCAGGC TATATCATTG GATGCTGACC GGCGGAAGCT CTATGTGACC 
TCCCAGTGGG AAGTGAGCCA GGTACCCCTG GACATGTGTG AGGTCTACAG CGGGGGCTGC 
CATGGCTGCC TCATGTCCCG AGACCCCTAC TGTGGCTGGG ACCAGGACCG CTGCGTGTCT 
ATCTACAGCT CCCAACGATC AGTGCTGCAG TCCATTAATC CAGCGGAGCC ACACAGAGAG 
TGTCCCAACC CTAAACCAGA CGAGGCTCCA TTGCAGAAGG TTTCCCTGGC TCGGAACTCT 
CGATACTACC TGACCTGCCC CATGGAGTCC CGCCACGCCA CTTACTTATG GCGCCATGAG 
GAGAATGTGG AACAGAGCTG TGAGCCAGGC CACCAAAGCC CTAGCTGCAT CCTGTTCATT 
GAGAACCTCA CGGCCCGTCA GTATGGCCAC TACCGCTGCG AGGCCCAAGA GGGCTCCTAC 
CTCCGTGAGG CTCAACACTG GGAGCTGCTG CCAGAGGACA GAGCACTGGC CGAACAACTA 
ATGGGCCATG CCCGGGCCCT GGCCGCCTCC TTCTGGCTGG GGGTCCTGCC CACACTCATA 
CTTGGTCTGC TGGTTCACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006510951.1
CDS384..1904
Translation

Target ORF information:

RefSeq Version XM_006510888.3
Organism Mus musculus(house mouse)
Definition Mus musculus sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006510888.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCACTTG GTGAGATGAA AGGCTATGCC CCCTTCAGCC CGGATGAGAA CTCCCTGGTT 
CTGTTTGAAG GAGATGAAGT GTACTCTACC ATCCGGAAGC AGGAATACAA CGGGAAGATC 
CCTCGGTTTC GACGCATTCG GGGCGAGAGT GAACTGTACA CAAGTGATAC AGTCATGCAG 
AACCCACAGT TCATCAAGGC CACCATTGTG CACCAAGACC AAGCCTATGA TGATAAGATC 
TACTACTTCT TCCGAGAAGA CAACCCTGAC AAGAACCCCG AGGCTCCTCT CAATGTGTCC 
CGAGTAGCCC AGTTGTGCAG GGGGGACCAG GGTGGTGAGA GTTCGTTGTC TGTCTCCAAG 
TGGAACACCT TCCTGAAAGC CATGTTGGTC TGCAGCGATG CAGCCACCAA CAGGAACTTC 
AATCGGCTGC AAGATGTCTT CCTGCTCCCT GACCCCAGTG GCCAGTGGAG AGATACCAGG 
GTCTATGGCG TTTTCTCCAA CCCCTGGAAC TACTCAGCTG TCTGCGTGTA TTCGCTTGGT 
GACATTGACA GAGTCTTCCG TACCTCATCG CTCAAAGGCT ACCACATGGG CCTTCCCAAC 
CCTCGACCTG GCATGTGCCT CCCAAAAAAG CAGCCCATAC CCACAGAAAC CTTCCAGGTA 
GCTGATAGTC ACCCAGAGGT GGCTCAGAGG GTGGAACCTA TGGGGCCACT GAAGACACCA 
TTGTTCCATT CTAAGTACCA TTACCAGAAA GTGGTCGTTC ACCGCATGCA AGCCAGCAAT 
GGAGAGACCT TCCATGTGCT TTATCTAACC ACAGACAGGG GCACCATTCA CAAGGTGGTG 
GAATCAGGGG ACCAGGACCA TAGCTTTGTC TTCAATATCA TGGAGATCCA GCCCTTTCAC 
CGTGCAGCGG CCATCCAGGC TATATCATTG GATGCTGACC GGCGGAAGCT CTATGTGACC 
TCCCAGTGGG AAGTGAGCCA GGTACCCCTG GACATGTGTG AGGTCTACAG CGGGGGCTGC 
CATGGCTGCC TCATGTCCCG AGACCCCTAC TGTGGCTGGG ACCAGGACCG CTGCGTGTCT 
ATCTACAGCT CCCAACGATC AGTGCTGCAG TCCATTAATC CAGCGGAGCC ACACAGAGAG 
TGTCCCAACC CTAAACCAGA CGAGGCTCCA TTGCAGAAGG TTTCCCTGGC TCGGAACTCT 
CGATACTACC TGACCTGCCC CATGGAGTCC CGCCACGCCA CTTACTTATG GCGCCATGAG 
GAGAATGTGG AACAGAGCTG TGAGCCAGGC CACCAAAGCC CTAGCTGCAT CCTGTTCATT 
GAGAACCTCA CGGCCCGTCA GTATGGCCAC TACCGCTGCG AGGCCCAAGA GGGCTCCTAC 
CTCCGTGAGG CTCAACACTG GGAGCTGCTG CCAGAGGACA GAGCACTGGC CGAACAACTA 
ATGGGCCATG CCCGGGCCCT GGCCGCCTCC TTCTGGCTGG GGGTCCTGCC CACACTCATA 
CTTGGTCTGC TGGTTCACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu90110
Clone ID Related Accession (Same CDS sequence) XM_030244149.1
Accession Version XM_030244149.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1347bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product semaphorin-7A isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCAGAACC CACAGTTCAT CAAGGCCACC ATTGTGCACC AAGACCAAGC CTATGATGAT 
AAGATCTACT ACTTCTTCCG AGAAGACAAC CCTGACAAGA ACCCCGAGGC TCCTCTCAAT 
GTGTCCCGAG TAGCCCAGTT GTGCAGGGGG GACCAGGGTG GTGAGAGTTC GTTGTCTGTC 
TCCAAGTGGA ACACCTTCCT GAAAGCCATG TTGGTCTGCA GCGATGCAGC CACCAACAGG 
AACTTCAATC GGCTGCAAGA TGTCTTCCTG CTCCCTGACC CCAGTGGCCA GTGGAGAGAT 
ACCAGGGTCT ATGGCGTTTT CTCCAACCCC TGGAACTACT CAGCTGTCTG CGTGTATTCG 
CTTGGTGACA TTGACAGAGT CTTCCGTACC TCATCGCTCA AAGGCTACCA CATGGGCCTT 
CCCAACCCTC GACCTGGCAT GTGCCTCCCA AAAAAGCAGC CCATACCCAC AGAAACCTTC 
CAGGTAGCTG ATAGTCACCC AGAGGTGGCT CAGAGGGTGG AACCTATGGG GCCACTGAAG 
ACACCATTGT TCCATTCTAA GTACCATTAC CAGAAAGTGG TCGTTCACCG CATGCAAGCC 
AGCAATGGAG AGACCTTCCA TGTGCTTTAT CTAACCACAG ACAGGGGCAC CATTCACAAG 
GTGGTGGAAT CAGGGGACCA GGACCATAGC TTTGTCTTCA ATATCATGGA GATCCAGCCC 
TTTCACCGTG CAGCGGCCAT CCAGGCTATA TCATTGGATG CTGACCGGCG GAAGCTCTAT 
GTGACCTCCC AGTGGGAAGT GAGCCAGGTA CCCCTGGACA TGTGTGAGGT CTACAGCGGG 
GGCTGCCATG GCTGCCTCAT GTCCCGAGAC CCCTACTGTG GCTGGGACCA GGACCGCTGC 
GTGTCTATCT ACAGCTCCCA ACGATCAGTG CTGCAGTCCA TTAATCCAGC GGAGCCACAC 
AGAGAGTGTC CCAACCCTAA ACCAGACGAG GCTCCATTGC AGAAGGTTTC CCTGGCTCGG 
AACTCTCGAT ACTACCTGAC CTGCCCCATG GAGTCCCGCC ACGCCACTTA CTTATGGCGC 
CATGAGGAGA ATGTGGAACA GAGCTGTGAG CCAGGCCACC AAAGCCCTAG CTGCATCCTG 
TTCATTGAGA ACCTCACGGC CCGTCAGTAT GGCCACTACC GCTGCGAGGC CCAAGAGGGC 
TCCTACCTCC GTGAGGCTCA ACACTGGGAG CTGCTGCCAG AGGACAGAGC ACTGGCCGAA 
CAACTAATGG GCCATGCCCG GGCCCTGGCC GCCTCCTTCT GGCTGGGGGT CCTGCCCACA 
CTCATACTTG GTCTGCTGGT TCACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_030100009.1
CDS1116..2462
Translation

Target ORF information:

RefSeq Version XM_030244149.1
Organism Mus musculus(house mouse)
Definition Mus musculus sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_030244149.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCAGAACC CACAGTTCAT CAAGGCCACC ATTGTGCACC AAGACCAAGC CTATGATGAT 
AAGATCTACT ACTTCTTCCG AGAAGACAAC CCTGACAAGA ACCCCGAGGC TCCTCTCAAT 
GTGTCCCGAG TAGCCCAGTT GTGCAGGGGG GACCAGGGTG GTGAGAGTTC GTTGTCTGTC 
TCCAAGTGGA ACACCTTCCT GAAAGCCATG TTGGTCTGCA GCGATGCAGC CACCAACAGG 
AACTTCAATC GGCTGCAAGA TGTCTTCCTG CTCCCTGACC CCAGTGGCCA GTGGAGAGAT 
ACCAGGGTCT ATGGCGTTTT CTCCAACCCC TGGAACTACT CAGCTGTCTG CGTGTATTCG 
CTTGGTGACA TTGACAGAGT CTTCCGTACC TCATCGCTCA AAGGCTACCA CATGGGCCTT 
CCCAACCCTC GACCTGGCAT GTGCCTCCCA AAAAAGCAGC CCATACCCAC AGAAACCTTC 
CAGGTAGCTG ATAGTCACCC AGAGGTGGCT CAGAGGGTGG AACCTATGGG GCCACTGAAG 
ACACCATTGT TCCATTCTAA GTACCATTAC CAGAAAGTGG TCGTTCACCG CATGCAAGCC 
AGCAATGGAG AGACCTTCCA TGTGCTTTAT CTAACCACAG ACAGGGGCAC CATTCACAAG 
GTGGTGGAAT CAGGGGACCA GGACCATAGC TTTGTCTTCA ATATCATGGA GATCCAGCCC 
TTTCACCGTG CAGCGGCCAT CCAGGCTATA TCATTGGATG CTGACCGGCG GAAGCTCTAT 
GTGACCTCCC AGTGGGAAGT GAGCCAGGTA CCCCTGGACA TGTGTGAGGT CTACAGCGGG 
GGCTGCCATG GCTGCCTCAT GTCCCGAGAC CCCTACTGTG GCTGGGACCA GGACCGCTGC 
GTGTCTATCT ACAGCTCCCA ACGATCAGTG CTGCAGTCCA TTAATCCAGC GGAGCCACAC 
AGAGAGTGTC CCAACCCTAA ACCAGACGAG GCTCCATTGC AGAAGGTTTC CCTGGCTCGG 
AACTCTCGAT ACTACCTGAC CTGCCCCATG GAGTCCCGCC ACGCCACTTA CTTATGGCGC 
CATGAGGAGA ATGTGGAACA GAGCTGTGAG CCAGGCCACC AAAGCCCTAG CTGCATCCTG 
TTCATTGAGA ACCTCACGGC CCGTCAGTAT GGCCACTACC GCTGCGAGGC CCAAGAGGGC 
TCCTACCTCC GTGAGGCTCA ACACTGGGAG CTGCTGCCAG AGGACAGAGC ACTGGCCGAA 
CAACTAATGG GCCATGCCCG GGCCCTGGCC GCCTCCTTCT GGCTGGGGGT CCTGCCCACA 
CTCATACTTG GTCTGCTGGT TCACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42610
Clone ID Related Accession (Same CDS sequence) XM_006510889.2 , XM_006510888.3 , XM_006510889.3
Accession Version XM_006510889.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1521bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product semaphorin-7A isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510889.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCACTTG GTGAGATGAA AGGCTATGCC CCCTTCAGCC CGGATGAGAA CTCCCTGGTT 
CTGTTTGAAG GAGATGAAGT GTACTCTACC ATCCGGAAGC AGGAATACAA CGGGAAGATC 
CCTCGGTTTC GACGCATTCG GGGCGAGAGT GAACTGTACA CAAGTGATAC AGTCATGCAG 
AACCCACAGT TCATCAAGGC CACCATTGTG CACCAAGACC AAGCCTATGA TGATAAGATC 
TACTACTTCT TCCGAGAAGA CAACCCTGAC AAGAACCCCG AGGCTCCTCT CAATGTGTCC 
CGAGTAGCCC AGTTGTGCAG GGGGGACCAG GGTGGTGAGA GTTCGTTGTC TGTCTCCAAG 
TGGAACACCT TCCTGAAAGC CATGTTGGTC TGCAGCGATG CAGCCACCAA CAGGAACTTC 
AATCGGCTGC AAGATGTCTT CCTGCTCCCT GACCCCAGTG GCCAGTGGAG AGATACCAGG 
GTCTATGGCG TTTTCTCCAA CCCCTGGAAC TACTCAGCTG TCTGCGTGTA TTCGCTTGGT 
GACATTGACA GAGTCTTCCG TACCTCATCG CTCAAAGGCT ACCACATGGG CCTTCCCAAC 
CCTCGACCTG GCATGTGCCT CCCAAAAAAG CAGCCCATAC CCACAGAAAC CTTCCAGGTA 
GCTGATAGTC ACCCAGAGGT GGCTCAGAGG GTGGAACCTA TGGGGCCACT GAAGACACCA 
TTGTTCCATT CTAAGTACCA TTACCAGAAA GTGGTCGTTC ACCGCATGCA AGCCAGCAAT 
GGAGAGACCT TCCATGTGCT TTATCTAACC ACAGACAGGG GCACCATTCA CAAGGTGGTG 
GAATCAGGGG ACCAGGACCA TAGCTTTGTC TTCAATATCA TGGAGATCCA GCCCTTTCAC 
CGTGCAGCGG CCATCCAGGC TATATCATTG GATGCTGACC GGCGGAAGCT CTATGTGACC 
TCCCAGTGGG AAGTGAGCCA GGTACCCCTG GACATGTGTG AGGTCTACAG CGGGGGCTGC 
CATGGCTGCC TCATGTCCCG AGACCCCTAC TGTGGCTGGG ACCAGGACCG CTGCGTGTCT 
ATCTACAGCT CCCAACGATC AGTGCTGCAG TCCATTAATC CAGCGGAGCC ACACAGAGAG 
TGTCCCAACC CTAAACCAGA CGAGGCTCCA TTGCAGAAGG TTTCCCTGGC TCGGAACTCT 
CGATACTACC TGACCTGCCC CATGGAGTCC CGCCACGCCA CTTACTTATG GCGCCATGAG 
GAGAATGTGG AACAGAGCTG TGAGCCAGGC CACCAAAGCC CTAGCTGCAT CCTGTTCATT 
GAGAACCTCA CGGCCCGTCA GTATGGCCAC TACCGCTGCG AGGCCCAAGA GGGCTCCTAC 
CTCCGTGAGG CTCAACACTG GGAGCTGCTG CCAGAGGACA GAGCACTGGC CGAACAACTA 
ATGGGCCATG CCCGGGCCCT GGCCGCCTCC TTCTGGCTGG GGGTCCTGCC CACACTCATA 
CTTGGTCTGC TGGTTCACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006510952.1
CDS404..1924
Translation

Target ORF information:

RefSeq Version XM_006510889.3
Organism Mus musculus(house mouse)
Definition Mus musculus sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006510889.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCACTTG GTGAGATGAA AGGCTATGCC CCCTTCAGCC CGGATGAGAA CTCCCTGGTT 
CTGTTTGAAG GAGATGAAGT GTACTCTACC ATCCGGAAGC AGGAATACAA CGGGAAGATC 
CCTCGGTTTC GACGCATTCG GGGCGAGAGT GAACTGTACA CAAGTGATAC AGTCATGCAG 
AACCCACAGT TCATCAAGGC CACCATTGTG CACCAAGACC AAGCCTATGA TGATAAGATC 
TACTACTTCT TCCGAGAAGA CAACCCTGAC AAGAACCCCG AGGCTCCTCT CAATGTGTCC 
CGAGTAGCCC AGTTGTGCAG GGGGGACCAG GGTGGTGAGA GTTCGTTGTC TGTCTCCAAG 
TGGAACACCT TCCTGAAAGC CATGTTGGTC TGCAGCGATG CAGCCACCAA CAGGAACTTC 
AATCGGCTGC AAGATGTCTT CCTGCTCCCT GACCCCAGTG GCCAGTGGAG AGATACCAGG 
GTCTATGGCG TTTTCTCCAA CCCCTGGAAC TACTCAGCTG TCTGCGTGTA TTCGCTTGGT 
GACATTGACA GAGTCTTCCG TACCTCATCG CTCAAAGGCT ACCACATGGG CCTTCCCAAC 
CCTCGACCTG GCATGTGCCT CCCAAAAAAG CAGCCCATAC CCACAGAAAC CTTCCAGGTA 
GCTGATAGTC ACCCAGAGGT GGCTCAGAGG GTGGAACCTA TGGGGCCACT GAAGACACCA 
TTGTTCCATT CTAAGTACCA TTACCAGAAA GTGGTCGTTC ACCGCATGCA AGCCAGCAAT 
GGAGAGACCT TCCATGTGCT TTATCTAACC ACAGACAGGG GCACCATTCA CAAGGTGGTG 
GAATCAGGGG ACCAGGACCA TAGCTTTGTC TTCAATATCA TGGAGATCCA GCCCTTTCAC 
CGTGCAGCGG CCATCCAGGC TATATCATTG GATGCTGACC GGCGGAAGCT CTATGTGACC 
TCCCAGTGGG AAGTGAGCCA GGTACCCCTG GACATGTGTG AGGTCTACAG CGGGGGCTGC 
CATGGCTGCC TCATGTCCCG AGACCCCTAC TGTGGCTGGG ACCAGGACCG CTGCGTGTCT 
ATCTACAGCT CCCAACGATC AGTGCTGCAG TCCATTAATC CAGCGGAGCC ACACAGAGAG 
TGTCCCAACC CTAAACCAGA CGAGGCTCCA TTGCAGAAGG TTTCCCTGGC TCGGAACTCT 
CGATACTACC TGACCTGCCC CATGGAGTCC CGCCACGCCA CTTACTTATG GCGCCATGAG 
GAGAATGTGG AACAGAGCTG TGAGCCAGGC CACCAAAGCC CTAGCTGCAT CCTGTTCATT 
GAGAACCTCA CGGCCCGTCA GTATGGCCAC TACCGCTGCG AGGCCCAAGA GGGCTCCTAC 
CTCCGTGAGG CTCAACACTG GGAGCTGCTG CCAGAGGACA GAGCACTGGC CGAACAACTA 
ATGGGCCATG CCCGGGCCCT GGCCGCCTCC TTCTGGCTGG GGGTCCTGCC CACACTCATA 
CTTGGTCTGC TGGTTCACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Vascular Semaphorin 7A Upregulation by Disturbed Flow Promotes Atherosclerosis Through Endothelial ?1 Integrin.
Arteriosclerosis, thrombosis, and vascular biology(2017 Dec)
Hu S,Liu Y,You T,Heath J,Xu L,Zheng X,Wang A,Wang Y,Li F,Yang F,Cao Y,Zhang H,van Gils JM,van Zonneveld AJ,Jo H,Wu Q,Zhang Y,Tang C,Zhu L


book

Semaphorin7A aggravates coxsackievirusB3-induced viral myocarditis by increasing ?1?1-integrin macrophages and subsequent enhanced inflammatory response.
Journal of molecular and cellular cardiology11448-57(2017 Nov)
Wu X,Meng Y,Wang C,Yue Y,Dong C,Xiong S


book

Suppression of tumor-derived Semaphorin?7A and genetic ablation of host-derived Semaphorin?7A impairs tumor progression in a murine model of advanced breast carcinoma.
International journal of oncology51(5)1395-1404(2017 Nov)
Garcia-Areas R,Libreros S,Simoes M,Castro-Silva C,Gazaniga N,Amat S,Jaczewska J,Keating P,Schilling K,Brito M,Wojcikiewicz EP,Iragavarpu-Charyulu V


book

Transcriptional repression of Plxnc1 by Lmx1a and Lmx1b directs topographic dopaminergic circuit formation.
Nature communications8(1)933(2017 10)
Chabrat A,Brisson G,Doucet-Beaupr? H,Salesse C,Schaan Profes M,Dovonou A,Akitegetse C,Charest J,Lemstra S,C?t? D,Pasterkamp RJ,Abrudan MI,Metzakopian E,Ang SL,L?vesque M