Supt6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Supt6
Entrez Gene ID	20926
Full Name	suppressor of Ty 6
Synonyms	5131400N11Rik,AI132449,SPT6,Supt6h,mKIAA0162
General protein information	Preferred Names suppressor of Ty 6 Names transcription elongation factor SPT6 suppressor of Ty 6 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B5\|11 46.74 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006532743.4	XP_006532806.1	transcription elongation factor SPT6 isoform X1
NM_009297.2	NP_033323.2	transcription elongation factor SPT6
XM_006532743.3	XP_006532806.1	transcription elongation factor SPT6 isoform X1

Pathways from BioSystems

Homologies

	SUPT6H	XP_001106850.2
Canis lupus familiaris (dog)	SUPT6H	XP_537747.2
Caenorhabditis elegans (roundworm)	emb-5	NP_497969.1
Magnaporthe oryzae (rice blast fungus)	MGG_11491	XP_003718184.1
Arabidopsis thaliana (thale cress)	AT1G63210	NP_176510.1
Gallus gallus (chicken)	SUPT6H	XP_423183.3
Schizosaccharomyces pombe (fission yeast)	spt6	NP_594487.2
Drosophila melanogaster (fruit fly)	Spt6	NP_651962.2
Kluyveromyces lactis	KLLA0B11385g	XP_452036.1
Eremothecium gossypii	AGOS_AAR031W	NP_982572.2
Neurospora crassa	NCU04611	XP_958854.1
Arabidopsis thaliana (thale cress)	GTB1	NP_001077775.1
Xenopus tropicalis (tropical clawed frog)	supt6h	NP_001072665.1
Homo sapiens (human)	SUPT6H	NP_003161.2
Pan troglodytes (chimpanzee)	SUPT6H	XP_001143115.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003619	XP_313379.5
Saccharomyces cerevisiae (baker's yeast)	SPT6	NP_011631.1
Bos taurus (cattle)	SUPT6H	NP_001180055.1
Mus musculus (house mouse)	Supt6	NP_033323.2
Rattus norvegicus (Norway rat)	Supt6h	NP_001178749.1
Danio rerio (zebrafish)	supt6h	NP_660094.1

Gene Ontology
Bibliography

Related articles in PubMed

The Elongation Factor Spt6 Maintains ESC Pluripotency by Controlling Super-Enhancers and Counteracting Polycomb Proteins.
Wang AH, Juan AH, Ko KD, Tsai PF, Zare H, Dell'Orso S, Sartorelli V
Molecular cell68(2)398-413.e6(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Biochemical as well as functional experiments revealed that Spt6 could compete for binding of the PRC2 methyltransferase Ezh2 to Suz12 and reduce PRC2 chromatin engagement.
Title: The Elongation Factor Spt6 Maintains ESC Pluripotency by Controlling Super-Enhancers and Counteracting Polycomb Proteins.

These data indicate that, through cooperation with PolII and KDM6A, Spt6 orchestrates removal of H3K27me3, thus controlling developmental gene expression and cell differentiation.
Title: The histone chaperone Spt6 coordinates histone H3K27 demethylation and myogenesis.

Knockdown of Spt6 resulted in severe reduction of class switch recombination in the endogenous Ig locus in B cells.
Title: Histone chaperone Spt6 is required for class switch recombination but not somatic hypermutation.

recombinant Spt6 binds selectively to a stretch of uninterrupted consensus repeats located in the N-terminal half of the CTD and recruits Iws1
Title: The Iws1:Spt6:CTD complex controls cotranscriptional mRNA biosynthesis and HYPB/Setd2-mediated histone H3K36 methylation.

Thus binding of Spt6 to Ser2-P RNAPII provides a cotranscriptional mechanism to recruit Iws1, REF1/Aly, and associated mRNA processing, surveillance, and export factors to responsive genes.
Title: The Spt6 SH2 domain binds Ser2-P RNAPII to direct Iws1-dependent mRNA splicing and export.

The following Supt6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Supt6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu45932	XM_006532743.4 Latest version!	Mus musculus SPT6, histone chaperone and transcription elongation factor (Supt6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu21312	NM_009297.2 Latest version!	Mus musculus SPT6, histone chaperone and transcription elongation factor (Supt6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1266.30 $1809.00
View Old Accession Versions >>
OMu45932	XM_006532743.3	Mus musculus suppressor of Ty 6 (Supt6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Supt6 ( XM_006532743.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532806.1 Mus musculus SPT6, histone chaperone and transcription elongation factor (Supt6), transcript variant X1, mRNA.

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CloneID	OMu45932
Clone ID Related Accession (Same CDS sequence)	XM_006532743.4 , XM_006532743.3
Accession Version	XM_006532743.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transcription elongation factor SPT6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532743.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGATG ATGATGAAGA TGATGAAGAG GAATATGGCA AAGAGGAACA CGAGAAGGAG 
GCCATTGCAG GGGAAATCTT CCAAGATGAA GAAGGGGAAG AAGGCCAGGA GGCTGTAGAG 
GCCCCCATGG CCCCTCCTGA CGAGGAGGAG GAAGATGATG AGGAGTCAGA CATTGACGAT 
TTCATTGTGG ATGATGATGG ACAGCCTCTG AAAAAACCCA AATGGCGAAA AAAGCTCCCT 
GGATATACAG ATGCAGCCCT GCAGGAAGCC CAGGAGATCT TCGGTGTGGA CTTTGACTAT 
GATGAATTTG AGAAATACAA TGAATACGAT GAAGAACTGG AAGAAGACTA TGAGTACGAG 
GATGATGAGA CTGAAGGTGA GATCCGAGTG CGCCCCAAGA AGACCACCAA GAAACGTGTG 
AGTCGCAGGA GCATCTTTGA GATGTACGAG CCTAGTGAGC TGGAGAGCAG CCACCTCACA 
GACCAGGACA ATGAAATCCG AGCCACTGAC TTGCCTGAGA GGTTCCAGCT CCGCTCCATC 
CCAGTCAAGG CAGCTGAGGA TGATGAGCTA GAAGAGGAAG CTGACTGGAT CTATAGAAAT 
GCCTTCGCAA CACCGACCAT CTCCCTCCAG GACAGCTGTG ATTACCTAGA CCGTGGGCAG 
CCAACTAGTA GCTTCAGTCG TAAAGGGCCC AGCACAGTTC AGAAGATCAA AGAGGCCCTG 
GGCTTCATGC GGAATCAGCA TTTTGAGGTG CCTTTCATTG CCTTCTACCG AAAGGAGTAT 
GTGGAACCTG AGCTGCACAT CAATGACCTG TGGAGGGTGT GGCAGTGGGA TGAGAAGTGG 
ACTCAGCTGA GAATCCGCAA AGAGAACCTA ACACGGCTGT TTGAGAAGAT GCAGGCTTAT 
CAGTATGAGC AAATCTCTGC TGACCCTGAC AAACCTCTTG CTGACGGCAT CCGGGCTCTA 
GATACTACTG ACATGGAGAG GCTCAAAGAT GTTCAGTCAA TGGATGAACT GAAAGACGTC 
TACAACCATT TCTTACTTTA CTACGGGCGT GACATCCCCA AGATGCAGAA TGCTGCCAAG 
GCTAGCCGGA AGAAGCTGAA ACGCATAAAG GAGGATGGCG ATGAAGAAGG TGAAGGTGAG 
GAGGCTGAAG ATGAGGAACA GAGGGGACCA GAGCTCAAGC AAGCCTCCCG CCGAGACATG 
TATACCATCT GCCAGAGTGC CGGGCTAGAT GGCCTGGCCA AAAAGTTTGG CCTCACACCT 
GAGCAGTTTG GGGAGAACCT CCGTGACAGC TACCAGCGCC ACGAGACAGA GCAGTTCCCT 
GCAGAGCCTT TGGAGCTGGC CAAAGATTAC GTGTGCAGCC AGTTCCCTAC ACCAGAAGCT 
GTGCTTGAAG GTGCCCGATA CATGGTTGCA CTACAGATTG CCAGGGAGCC CCTTGTCAGA 
CAGGTGCTGA GACAAACCTT TCAGGAGAGA GCCAAGCTAA ACATAACCCC AACCAAGAAA 
GGTAGAAAGG ATGTGGATGA GGCCCACTAC GCTTACTCTT TTAAGTACTT AAAGAACAAG 
CCTGTTAAAG AGCTGAGAGA CGACCAGTTC CTCAAGATAG GCCTGGCTGA GGATGAAGGG 
CTGCTCACCA TTGACATCAG CATAGATATG AAGGGAGTGG AAGGGTATGG TAATGACCAG 
ACATATTTTG AGGAGATCAA GCAGTTTTAC TACCGGGATG AGTTCAGCCA CCAAGTACAG 
GAATGGAACC GGCAGCGCAC TATGGCCATT GAGCGAGCTC TGCAGCAGTT CCTCTACGTG 
CAAATGGCCA AAGAACTTAA GAACAAGCTG CTAGCTGAGG CCAGGGAGAG TGTTGTGAAG 
GCCTGTAGCC GAAAGCTCTA CAACTGGTTG AGAGTGGCAC CCTACCGTCC AGATCAGCAG 
GTAGAGGAGG ACGACGACTT TATGGATGAG AACCAGGGGA AGGGCATCCG AGTCCTAGGC 
ATTGCTTTCT CCTCTGCCAG AGATCACCCT GTGTTCTGTG CCCTAGTCAA TGGTGAAGGA 
GAAGTGACTG ATTTCCTCCG CCTGCCCCAT TTTACCAAGC GGCGAACTGC ATGGAGAGAA 
GAGGAGCGGG AAAAGAAGGC TCAAGATATT GAAACCCTAA AGAAGTTTCT TGTGAATAAG 
AAGCCTCATG TGGTCACTAT CGCAGGAGAG AACAGGGATG CCCAGATGCT GACTGAGGAT 
GTGAAGCGGA TAGTGCATGA GCTGGACCAA GGCCAACAGC TGTCTTCTAT TGGGGTGGAG 
CTGGTTGACA ATGAGCTGGC CATTCTGTAC ATGAACAGCA AGAAATCAGA GGCAGAGTTC 
CGGGATTATC CACCAGTTTT GAGACAAGCT GTCTCTCTAG CCCGGCGTAT CCAAGACCCT 
CTGATAGAAT TTGCCCAGGT GTGCAGCTCA GATGAAGACA TCCTGTGTCT CAAGTTTCAT 
CCCTTGCAGG AGCATGTTGT GAAAGAGGAG CTGCTTAACG CCTTGTACTG TGAATTCATC 
AACCGGGTCA ATGAGGTCGG GGTGGATGTC AACCGTGCCA TTGCCCATCC TTACAGCCAG 
GCCTTGATCC AGTATGTCTG TGGCCTGGGA CCCCGAAAAG GGACCCACCT GCTGAAGATT 
TTGAAACAGA ACAACACTCG ACTCGAGAGC CGGACTCAGC TGGTCACCAT GTGTCATATG 
GGTCCCAAAG TCTTCATGAA TTGTGCTGGC TTCCTCAAGA TCGACACTGC TTCTCTGGGG 
GACAGCACTG ACTCATACAT TGAAGTTCTT GATGGCTCTC GAGTCCACCC TGAAACGTAC 
GAGTGGGCCA GGAAGATGGC AGTGGATGCC TTAGAATACG ATGAGTCTGC AGAAGATGCC 
AATCCTGCTG GAGCCCTTGA GGAAATCTTG GAAAACCCAG AGCGGCTGAA GGATTTAGAT 
CTTGATGCCT TTGCAGAAGA ACTAGAGAGA CAGGGCTATG GTGACAAGCA CATCACACTG 
TATGACATCC GAGCAGAGCT CAGTTGTAGG TATAAGGACC TCCGGACAGC CTACCGCTCT 
CCTAACACAG AGGAGATCTT CAATATGTTA ACCAAAGAGA CGCCAGAGAC CTTCTACATT 
GGAAAGCTTA TCATCTGCAA TGTTACCGGC ATTGCTCACA GGCGCCCACA GGGTGAGAGC 
TATGACCAGG CCATCCGAAA TGATGAGACA GGGCTGTGGC AGTGCCCCTT CTGTCAACAA 
GACAACTTCC CTGAGCTAAG CGAGGTCTGG AACCATTTTG ACAGTGGATC ATGCCCTGGT 
CAGGCCATTG GTGTGAAAAC ACGGCTAGAC AATGGCGTCA CCGGCTTCAT CCCTACCAAA 
TTCCTCAGTG ACAAGGTGGT GAAGAGGCCA GAGGAGCGAG TGAAGGTGGG GATGACTGTT 
CACTGCCGGA TCATGAAGAT CGATATTGAA AAGTTCAGTG CAGACCTGAC CTGCCGGACC 
TCAGACCTCA TGGACAGGAA CAATGAGTGG AAACTGCCCA AGGACACCTA CTATGACTTT 
GATGCGGAAG CTGCAGACCA CAAGCAGGAA GAGGATATGA AACGAAAGCA GCAGCGGACC 
ACATACATCA AGCGAGTGAT TGCACACCCG TCCTTCCATA ACATAAATTT CAAGCAAGCA 
GAAAAGATGA TGGAGACCAT GGACCAAGGG GATGTTATCA TTCGGCCCAG CAGTAAAGGT 
GAAAACCATC TGACGGTGAC CTGGAAAGTC AGTGCTGGCA TCTACCAGCA CGTGGACGTG 
CGGGAAGAGG GCAAGGAGAA CGCCTTCAGC CTGGGAGCCA CTCTTTGGAT CAACAGTGAG 
GAATTTGAAG ACTTGGATGA GATTGTTGCC CGCTATGTCC AGCCCATGGC TTCCTTTGCT 
CGAGACCTTC TGAACCACAA ATATTACCAG GACTGTAGTG GTGGGGACCG AAAGAAATTA 
GAGGAGCTGC TCATCAAAAC TAAGAAGGAG AAGCCCACCT TCATCCCTTA CTTCATCTGT 
GCCTGCAAGG AGCTGCCCGG CAAGTTCCTC CTGGGATACC AGCCCCGGGG TAAACCCAGG 
ATAGAATATG TAACAGTGAC TCCTGAAGGA TTCCGGTACC GGGGCCAGAT TTTCCCAACT 
GTGAATGGAC TCTTCAGGTG GTTTAAGGAT CACTATCAGG ATCCTGTACC AGGCATCACT 
CCCAGTAGCA GTAACAGAAC ACGGACCCCT GCTTCTATCA ATGCTACCCC AGCCAACATC 
AATCTTGCAG ATCTCACACG GGCTGTGAAT GCCTTGCCTC AGAACATGAC TTCACAGATG 
TTCAGTGCCA TTGCTGCTGT GACAGGCCAG GGGCAGAACC CCAATGCTAC CCCAGCCCAG 
TGGGCATCCA GCCAGTATGG CTACGGTGGC AGCGGAGGAG GCAGCAGCGC CTACCATGTG 
TTCCCAACAC CAGCCCAGCA GCCAGTGGCC ACACCACTAA TGACCCCAAG CTACTCCTAT 
ACTACCCCGA GCCAGCCCAT TACCACCCCA CAATACCACC AGCTCCAGGC CAGCACCACC 
CCACAGTCTA CACAGGCCCA GCCCCAGCCT TCCTCCAGCT CCCGACAACG GCAACAGCAG 
CCAAAGTCCA ACAGCCATGC AGCTATCGAC TGGGGCAAGA TGGCCGAGCA GTGGCTACAG 
GAAAAGGAGG CAGAACGACG GAAACAGAAA CAGCGGCTGA CCCCACGACC CTCTCCCAGC 
CCCATGATCG AGAGCACCCC CATGTCCATT GCTGGCGATG CGACCCCACT CCTGGACGAG 
ATGGATCGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532806.1
CDS	598..5409
Translation	MSDDDEDDEEEYGKEEHEKEAIAGEIFQDEEGEEGQEAVEAPMAPPDEEEEDDEESDIDDFIVDDDGQPLKKPKWRKKLPGYTDAALQEAQEIFGVDFDYDEFEKYNEYDEELEEDYEYEDDETEGEIRVRPKKTTKKRVSRRSIFEMYEPSELESSHLTDQDNEIRATDLPERFQLRSIPVKAAEDDELEEEADWIYRNAFATPTISLQDSCDYLDRGQPTSSFSRKGPSTVQKIKEALGFMRNQHFEVPFIAFYRKEYVEPELHINDLWRVWQWDEKWTQLRIRKENLTRLFEKMQAYQYEQISADPDKPLADGIRALDTTDMERLKDVQSMDELKDVYNHFLLYYGRDIPKMQNAAKASRKKLKRIKEDGDEEGEGEEAEDEEQRGPELKQASRRDMYTICQSAGLDGLAKKFGLTPEQFGENLRDSYQRHETEQFPAEPLELAKDYVCSQFPTPEAVLEGARYMVALQIAREPLVRQVLRQTFQERAKLNITPTKKGRKDVDEAHYAYSFKYLKNKPVKELRDDQFLKIGLAEDEGLLTIDISIDMKGVEGYGNDQTYFEEIKQFYYRDEFSHQVQEWNRQRTMAIERALQQFLYVQMAKELKNKLLAEARESVVKACSRKLYNWLRVAPYRPDQQVEEDDDFMDENQGKGIRVLGIAFSSARDHPVFCALVNGEGEVTDFLRLPHFTKRRTAWREEEREKKAQDIETLKKFLVNKKPHVVTIAGENRDAQMLTEDVKRIVHELDQGQQLSSIGVELVDNELAILYMNSKKSEAEFRDYPPVLRQAVSLARRIQDPLIEFAQVCSSDEDILCLKFHPLQEHVVKEELLNALYCEFINRVNEVGVDVNRAIAHPYSQALIQYVCGLGPRKGTHLLKILKQNNTRLESRTQLVTMCHMGPKVFMNCAGFLKIDTASLGDSTDSYIEVLDGSRVHPETYEWARKMAVDALEYDESAEDANPAGALEEILENPERLKDLDLDAFAEELERQGYGDKHITLYDIRAELSCRYKDLRTAYRSPNTEEIFNMLTKETPETFYIGKLIICNVTGIAHRRPQGESYDQAIRNDETGLWQCPFCQQDNFPELSEVWNHFDSGSCPGQAIGVKTRLDNGVTGFIPTKFLSDKVVKRPEERVKVGMTVHCRIMKIDIEKFSADLTCRTSDLMDRNNEWKLPKDTYYDFDAEAADHKQEEDMKRKQQRTTYIKRVIAHPSFHNINFKQAEKMMETMDQGDVIIRPSSKGENHLTVTWKVSAGIYQHVDVREEGKENAFSLGATLWINSEEFEDLDEIVARYVQPMASFARDLLNHKYYQDCSGGDRKKLEELLIKTKKEKPTFIPYFICACKELPGKFLLGYQPRGKPRIEYVTVTPEGFRYRGQIFPTVNGLFRWFKDHYQDPVPGITPSSSNRTRTPASINATPANINLADLTRAVNALPQNMTSQMFSAIAAVTGQGQNPNATPAQWASSQYGYGGSGGGSSAYHVFPTPAQQPVATPLMTPSYSYTTPSQPITTPQYHQLQASTTPQSTQAQPQPSSSSRQRQQQPKSNSHAAIDWGKMAEQWLQEKEAERRKQKQRLTPRPSPSPMIESTPMSIAGDATPLLDEMDR

Target ORF information:

RefSeq Version	XM_006532743.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPT6, histone chaperone and transcription elongation factor (Supt6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532743.4

ORF Insert Sequence:

ATGTCTGATG ATGATGAAGA TGATGAAGAG GAATATGGCA AAGAGGAACA CGAGAAGGAG 
GCCATTGCAG GGGAAATCTT CCAAGATGAA GAAGGGGAAG AAGGCCAGGA GGCTGTAGAG 
GCCCCCATGG CCCCTCCTGA CGAGGAGGAG GAAGATGATG AGGAGTCAGA CATTGACGAT 
TTCATTGTGG ATGATGATGG ACAGCCTCTG AAAAAACCCA AATGGCGAAA AAAGCTCCCT 
GGATATACAG ATGCAGCCCT GCAGGAAGCC CAGGAGATCT TCGGTGTGGA CTTTGACTAT 
GATGAATTTG AGAAATACAA TGAATACGAT GAAGAACTGG AAGAAGACTA TGAGTACGAG 
GATGATGAGA CTGAAGGTGA GATCCGAGTG CGCCCCAAGA AGACCACCAA GAAACGTGTG 
AGTCGCAGGA GCATCTTTGA GATGTACGAG CCTAGTGAGC TGGAGAGCAG CCACCTCACA 
GACCAGGACA ATGAAATCCG AGCCACTGAC TTGCCTGAGA GGTTCCAGCT CCGCTCCATC 
CCAGTCAAGG CAGCTGAGGA TGATGAGCTA GAAGAGGAAG CTGACTGGAT CTATAGAAAT 
GCCTTCGCAA CACCGACCAT CTCCCTCCAG GACAGCTGTG ATTACCTAGA CCGTGGGCAG 
CCAACTAGTA GCTTCAGTCG TAAAGGGCCC AGCACAGTTC AGAAGATCAA AGAGGCCCTG 
GGCTTCATGC GGAATCAGCA TTTTGAGGTG CCTTTCATTG CCTTCTACCG AAAGGAGTAT 
GTGGAACCTG AGCTGCACAT CAATGACCTG TGGAGGGTGT GGCAGTGGGA TGAGAAGTGG 
ACTCAGCTGA GAATCCGCAA AGAGAACCTA ACACGGCTGT TTGAGAAGAT GCAGGCTTAT 
CAGTATGAGC AAATCTCTGC TGACCCTGAC AAACCTCTTG CTGACGGCAT CCGGGCTCTA 
GATACTACTG ACATGGAGAG GCTCAAAGAT GTTCAGTCAA TGGATGAACT GAAAGACGTC 
TACAACCATT TCTTACTTTA CTACGGGCGT GACATCCCCA AGATGCAGAA TGCTGCCAAG 
GCTAGCCGGA AGAAGCTGAA ACGCATAAAG GAGGATGGCG ATGAAGAAGG TGAAGGTGAG 
GAGGCTGAAG ATGAGGAACA GAGGGGACCA GAGCTCAAGC AAGCCTCCCG CCGAGACATG 
TATACCATCT GCCAGAGTGC CGGGCTAGAT GGCCTGGCCA AAAAGTTTGG CCTCACACCT 
GAGCAGTTTG GGGAGAACCT CCGTGACAGC TACCAGCGCC ACGAGACAGA GCAGTTCCCT 
GCAGAGCCTT TGGAGCTGGC CAAAGATTAC GTGTGCAGCC AGTTCCCTAC ACCAGAAGCT 
GTGCTTGAAG GTGCCCGATA CATGGTTGCA CTACAGATTG CCAGGGAGCC CCTTGTCAGA 
CAGGTGCTGA GACAAACCTT TCAGGAGAGA GCCAAGCTAA ACATAACCCC AACCAAGAAA 
GGTAGAAAGG ATGTGGATGA GGCCCACTAC GCTTACTCTT TTAAGTACTT AAAGAACAAG 
CCTGTTAAAG AGCTGAGAGA CGACCAGTTC CTCAAGATAG GCCTGGCTGA GGATGAAGGG 
CTGCTCACCA TTGACATCAG CATAGATATG AAGGGAGTGG AAGGGTATGG TAATGACCAG 
ACATATTTTG AGGAGATCAA GCAGTTTTAC TACCGGGATG AGTTCAGCCA CCAAGTACAG 
GAATGGAACC GGCAGCGCAC TATGGCCATT GAGCGAGCTC TGCAGCAGTT CCTCTACGTG 
CAAATGGCCA AAGAACTTAA GAACAAGCTG CTAGCTGAGG CCAGGGAGAG TGTTGTGAAG 
GCCTGTAGCC GAAAGCTCTA CAACTGGTTG AGAGTGGCAC CCTACCGTCC AGATCAGCAG 
GTAGAGGAGG ACGACGACTT TATGGATGAG AACCAGGGGA AGGGCATCCG AGTCCTAGGC 
ATTGCTTTCT CCTCTGCCAG AGATCACCCT GTGTTCTGTG CCCTAGTCAA TGGTGAAGGA 
GAAGTGACTG ATTTCCTCCG CCTGCCCCAT TTTACCAAGC GGCGAACTGC ATGGAGAGAA 
GAGGAGCGGG AAAAGAAGGC TCAAGATATT GAAACCCTAA AGAAGTTTCT TGTGAATAAG 
AAGCCTCATG TGGTCACTAT CGCAGGAGAG AACAGGGATG CCCAGATGCT GACTGAGGAT 
GTGAAGCGGA TAGTGCATGA GCTGGACCAA GGCCAACAGC TGTCTTCTAT TGGGGTGGAG 
CTGGTTGACA ATGAGCTGGC CATTCTGTAC ATGAACAGCA AGAAATCAGA GGCAGAGTTC 
CGGGATTATC CACCAGTTTT GAGACAAGCT GTCTCTCTAG CCCGGCGTAT CCAAGACCCT 
CTGATAGAAT TTGCCCAGGT GTGCAGCTCA GATGAAGACA TCCTGTGTCT CAAGTTTCAT 
CCCTTGCAGG AGCATGTTGT GAAAGAGGAG CTGCTTAACG CCTTGTACTG TGAATTCATC 
AACCGGGTCA ATGAGGTCGG GGTGGATGTC AACCGTGCCA TTGCCCATCC TTACAGCCAG 
GCCTTGATCC AGTATGTCTG TGGCCTGGGA CCCCGAAAAG GGACCCACCT GCTGAAGATT 
TTGAAACAGA ACAACACTCG ACTCGAGAGC CGGACTCAGC TGGTCACCAT GTGTCATATG 
GGTCCCAAAG TCTTCATGAA TTGTGCTGGC TTCCTCAAGA TCGACACTGC TTCTCTGGGG 
GACAGCACTG ACTCATACAT TGAAGTTCTT GATGGCTCTC GAGTCCACCC TGAAACGTAC 
GAGTGGGCCA GGAAGATGGC AGTGGATGCC TTAGAATACG ATGAGTCTGC AGAAGATGCC 
AATCCTGCTG GAGCCCTTGA GGAAATCTTG GAAAACCCAG AGCGGCTGAA GGATTTAGAT 
CTTGATGCCT TTGCAGAAGA ACTAGAGAGA CAGGGCTATG GTGACAAGCA CATCACACTG 
TATGACATCC GAGCAGAGCT CAGTTGTAGG TATAAGGACC TCCGGACAGC CTACCGCTCT 
CCTAACACAG AGGAGATCTT CAATATGTTA ACCAAAGAGA CGCCAGAGAC CTTCTACATT 
GGAAAGCTTA TCATCTGCAA TGTTACCGGC ATTGCTCACA GGCGCCCACA GGGTGAGAGC 
TATGACCAGG CCATCCGAAA TGATGAGACA GGGCTGTGGC AGTGCCCCTT CTGTCAACAA 
GACAACTTCC CTGAGCTAAG CGAGGTCTGG AACCATTTTG ACAGTGGATC ATGCCCTGGT 
CAGGCCATTG GTGTGAAAAC ACGGCTAGAC AATGGCGTCA CCGGCTTCAT CCCTACCAAA 
TTCCTCAGTG ACAAGGTGGT GAAGAGGCCA GAGGAGCGAG TGAAGGTGGG GATGACTGTT 
CACTGCCGGA TCATGAAGAT CGATATTGAA AAGTTCAGTG CAGACCTGAC CTGCCGGACC 
TCAGACCTCA TGGACAGGAA CAATGAGTGG AAACTGCCCA AGGACACCTA CTATGACTTT 
GATGCGGAAG CTGCAGACCA CAAGCAGGAA GAGGATATGA AACGAAAGCA GCAGCGGACC 
ACATACATCA AGCGAGTGAT TGCACACCCG TCCTTCCATA ACATAAATTT CAAGCAAGCA 
GAAAAGATGA TGGAGACCAT GGACCAAGGG GATGTTATCA TTCGGCCCAG CAGTAAAGGT 
GAAAACCATC TGACGGTGAC CTGGAAAGTC AGTGCTGGCA TCTACCAGCA CGTGGACGTG 
CGGGAAGAGG GCAAGGAGAA CGCCTTCAGC CTGGGAGCCA CTCTTTGGAT CAACAGTGAG 
GAATTTGAAG ACTTGGATGA GATTGTTGCC CGCTATGTCC AGCCCATGGC TTCCTTTGCT 
CGAGACCTTC TGAACCACAA ATATTACCAG GACTGTAGTG GTGGGGACCG AAAGAAATTA 
GAGGAGCTGC TCATCAAAAC TAAGAAGGAG AAGCCCACCT TCATCCCTTA CTTCATCTGT 
GCCTGCAAGG AGCTGCCCGG CAAGTTCCTC CTGGGATACC AGCCCCGGGG TAAACCCAGG 
ATAGAATATG TAACAGTGAC TCCTGAAGGA TTCCGGTACC GGGGCCAGAT TTTCCCAACT 
GTGAATGGAC TCTTCAGGTG GTTTAAGGAT CACTATCAGG ATCCTGTACC AGGCATCACT 
CCCAGTAGCA GTAACAGAAC ACGGACCCCT GCTTCTATCA ATGCTACCCC AGCCAACATC 
AATCTTGCAG ATCTCACACG GGCTGTGAAT GCCTTGCCTC AGAACATGAC TTCACAGATG 
TTCAGTGCCA TTGCTGCTGT GACAGGCCAG GGGCAGAACC CCAATGCTAC CCCAGCCCAG 
TGGGCATCCA GCCAGTATGG CTACGGTGGC AGCGGAGGAG GCAGCAGCGC CTACCATGTG 
TTCCCAACAC CAGCCCAGCA GCCAGTGGCC ACACCACTAA TGACCCCAAG CTACTCCTAT 
ACTACCCCGA GCCAGCCCAT TACCACCCCA CAATACCACC AGCTCCAGGC CAGCACCACC 
CCACAGTCTA CACAGGCCCA GCCCCAGCCT TCCTCCAGCT CCCGACAACG GCAACAGCAG 
CCAAAGTCCA ACAGCCATGC AGCTATCGAC TGGGGCAAGA TGGCCGAGCA GTGGCTACAG 
GAAAAGGAGG CAGAACGACG GAAACAGAAA CAGCGGCTGA CCCCACGACC CTCTCCCAGC 
CCCATGATCG AGAGCACCCC CATGTCCATT GCTGGCGATG CGACCCCACT CCTGGACGAG 
ATGGATCGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Supt6 ( XM_006532743.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532806.1 Mus musculus suppressor of Ty 6 (Supt6), transcript variant X1, mRNA.

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CloneID	OMu45932
Clone ID Related Accession (Same CDS sequence)	XM_006532743.4 , XM_006532743.3
Accession Version	XM_006532743.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transcription elongation factor SPT6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532743.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGATG ATGATGAAGA TGATGAAGAG GAATATGGCA AAGAGGAACA CGAGAAGGAG 
GCCATTGCAG GGGAAATCTT CCAAGATGAA GAAGGGGAAG AAGGCCAGGA GGCTGTAGAG 
GCCCCCATGG CCCCTCCTGA CGAGGAGGAG GAAGATGATG AGGAGTCAGA CATTGACGAT 
TTCATTGTGG ATGATGATGG ACAGCCTCTG AAAAAACCCA AATGGCGAAA AAAGCTCCCT 
GGATATACAG ATGCAGCCCT GCAGGAAGCC CAGGAGATCT TCGGTGTGGA CTTTGACTAT 
GATGAATTTG AGAAATACAA TGAATACGAT GAAGAACTGG AAGAAGACTA TGAGTACGAG 
GATGATGAGA CTGAAGGTGA GATCCGAGTG CGCCCCAAGA AGACCACCAA GAAACGTGTG 
AGTCGCAGGA GCATCTTTGA GATGTACGAG CCTAGTGAGC TGGAGAGCAG CCACCTCACA 
GACCAGGACA ATGAAATCCG AGCCACTGAC TTGCCTGAGA GGTTCCAGCT CCGCTCCATC 
CCAGTCAAGG CAGCTGAGGA TGATGAGCTA GAAGAGGAAG CTGACTGGAT CTATAGAAAT 
GCCTTCGCAA CACCGACCAT CTCCCTCCAG GACAGCTGTG ATTACCTAGA CCGTGGGCAG 
CCAACTAGTA GCTTCAGTCG TAAAGGGCCC AGCACAGTTC AGAAGATCAA AGAGGCCCTG 
GGCTTCATGC GGAATCAGCA TTTTGAGGTG CCTTTCATTG CCTTCTACCG AAAGGAGTAT 
GTGGAACCTG AGCTGCACAT CAATGACCTG TGGAGGGTGT GGCAGTGGGA TGAGAAGTGG 
ACTCAGCTGA GAATCCGCAA AGAGAACCTA ACACGGCTGT TTGAGAAGAT GCAGGCTTAT 
CAGTATGAGC AAATCTCTGC TGACCCTGAC AAACCTCTTG CTGACGGCAT CCGGGCTCTA 
GATACTACTG ACATGGAGAG GCTCAAAGAT GTTCAGTCAA TGGATGAACT GAAAGACGTC 
TACAACCATT TCTTACTTTA CTACGGGCGT GACATCCCCA AGATGCAGAA TGCTGCCAAG 
GCTAGCCGGA AGAAGCTGAA ACGCATAAAG GAGGATGGCG ATGAAGAAGG TGAAGGTGAG 
GAGGCTGAAG ATGAGGAACA GAGGGGACCA GAGCTCAAGC AAGCCTCCCG CCGAGACATG 
TATACCATCT GCCAGAGTGC CGGGCTAGAT GGCCTGGCCA AAAAGTTTGG CCTCACACCT 
GAGCAGTTTG GGGAGAACCT CCGTGACAGC TACCAGCGCC ACGAGACAGA GCAGTTCCCT 
GCAGAGCCTT TGGAGCTGGC CAAAGATTAC GTGTGCAGCC AGTTCCCTAC ACCAGAAGCT 
GTGCTTGAAG GTGCCCGATA CATGGTTGCA CTACAGATTG CCAGGGAGCC CCTTGTCAGA 
CAGGTGCTGA GACAAACCTT TCAGGAGAGA GCCAAGCTAA ACATAACCCC AACCAAGAAA 
GGTAGAAAGG ATGTGGATGA GGCCCACTAC GCTTACTCTT TTAAGTACTT AAAGAACAAG 
CCTGTTAAAG AGCTGAGAGA CGACCAGTTC CTCAAGATAG GCCTGGCTGA GGATGAAGGG 
CTGCTCACCA TTGACATCAG CATAGATATG AAGGGAGTGG AAGGGTATGG TAATGACCAG 
ACATATTTTG AGGAGATCAA GCAGTTTTAC TACCGGGATG AGTTCAGCCA CCAAGTACAG 
GAATGGAACC GGCAGCGCAC TATGGCCATT GAGCGAGCTC TGCAGCAGTT CCTCTACGTG 
CAAATGGCCA AAGAACTTAA GAACAAGCTG CTAGCTGAGG CCAGGGAGAG TGTTGTGAAG 
GCCTGTAGCC GAAAGCTCTA CAACTGGTTG AGAGTGGCAC CCTACCGTCC AGATCAGCAG 
GTAGAGGAGG ACGACGACTT TATGGATGAG AACCAGGGGA AGGGCATCCG AGTCCTAGGC 
ATTGCTTTCT CCTCTGCCAG AGATCACCCT GTGTTCTGTG CCCTAGTCAA TGGTGAAGGA 
GAAGTGACTG ATTTCCTCCG CCTGCCCCAT TTTACCAAGC GGCGAACTGC ATGGAGAGAA 
GAGGAGCGGG AAAAGAAGGC TCAAGATATT GAAACCCTAA AGAAGTTTCT TGTGAATAAG 
AAGCCTCATG TGGTCACTAT CGCAGGAGAG AACAGGGATG CCCAGATGCT GACTGAGGAT 
GTGAAGCGGA TAGTGCATGA GCTGGACCAA GGCCAACAGC TGTCTTCTAT TGGGGTGGAG 
CTGGTTGACA ATGAGCTGGC CATTCTGTAC ATGAACAGCA AGAAATCAGA GGCAGAGTTC 
CGGGATTATC CACCAGTTTT GAGACAAGCT GTCTCTCTAG CCCGGCGTAT CCAAGACCCT 
CTGATAGAAT TTGCCCAGGT GTGCAGCTCA GATGAAGACA TCCTGTGTCT CAAGTTTCAT 
CCCTTGCAGG AGCATGTTGT GAAAGAGGAG CTGCTTAACG CCTTGTACTG TGAATTCATC 
AACCGGGTCA ATGAGGTCGG GGTGGATGTC AACCGTGCCA TTGCCCATCC TTACAGCCAG 
GCCTTGATCC AGTATGTCTG TGGCCTGGGA CCCCGAAAAG GGACCCACCT GCTGAAGATT 
TTGAAACAGA ACAACACTCG ACTCGAGAGC CGGACTCAGC TGGTCACCAT GTGTCATATG 
GGTCCCAAAG TCTTCATGAA TTGTGCTGGC TTCCTCAAGA TCGACACTGC TTCTCTGGGG 
GACAGCACTG ACTCATACAT TGAAGTTCTT GATGGCTCTC GAGTCCACCC TGAAACGTAC 
GAGTGGGCCA GGAAGATGGC AGTGGATGCC TTAGAATACG ATGAGTCTGC AGAAGATGCC 
AATCCTGCTG GAGCCCTTGA GGAAATCTTG GAAAACCCAG AGCGGCTGAA GGATTTAGAT 
CTTGATGCCT TTGCAGAAGA ACTAGAGAGA CAGGGCTATG GTGACAAGCA CATCACACTG 
TATGACATCC GAGCAGAGCT CAGTTGTAGG TATAAGGACC TCCGGACAGC CTACCGCTCT 
CCTAACACAG AGGAGATCTT CAATATGTTA ACCAAAGAGA CGCCAGAGAC CTTCTACATT 
GGAAAGCTTA TCATCTGCAA TGTTACCGGC ATTGCTCACA GGCGCCCACA GGGTGAGAGC 
TATGACCAGG CCATCCGAAA TGATGAGACA GGGCTGTGGC AGTGCCCCTT CTGTCAACAA 
GACAACTTCC CTGAGCTAAG CGAGGTCTGG AACCATTTTG ACAGTGGATC ATGCCCTGGT 
CAGGCCATTG GTGTGAAAAC ACGGCTAGAC AATGGCGTCA CCGGCTTCAT CCCTACCAAA 
TTCCTCAGTG ACAAGGTGGT GAAGAGGCCA GAGGAGCGAG TGAAGGTGGG GATGACTGTT 
CACTGCCGGA TCATGAAGAT CGATATTGAA AAGTTCAGTG CAGACCTGAC CTGCCGGACC 
TCAGACCTCA TGGACAGGAA CAATGAGTGG AAACTGCCCA AGGACACCTA CTATGACTTT 
GATGCGGAAG CTGCAGACCA CAAGCAGGAA GAGGATATGA AACGAAAGCA GCAGCGGACC 
ACATACATCA AGCGAGTGAT TGCACACCCG TCCTTCCATA ACATAAATTT CAAGCAAGCA 
GAAAAGATGA TGGAGACCAT GGACCAAGGG GATGTTATCA TTCGGCCCAG CAGTAAAGGT 
GAAAACCATC TGACGGTGAC CTGGAAAGTC AGTGCTGGCA TCTACCAGCA CGTGGACGTG 
CGGGAAGAGG GCAAGGAGAA CGCCTTCAGC CTGGGAGCCA CTCTTTGGAT CAACAGTGAG 
GAATTTGAAG ACTTGGATGA GATTGTTGCC CGCTATGTCC AGCCCATGGC TTCCTTTGCT 
CGAGACCTTC TGAACCACAA ATATTACCAG GACTGTAGTG GTGGGGACCG AAAGAAATTA 
GAGGAGCTGC TCATCAAAAC TAAGAAGGAG AAGCCCACCT TCATCCCTTA CTTCATCTGT 
GCCTGCAAGG AGCTGCCCGG CAAGTTCCTC CTGGGATACC AGCCCCGGGG TAAACCCAGG 
ATAGAATATG TAACAGTGAC TCCTGAAGGA TTCCGGTACC GGGGCCAGAT TTTCCCAACT 
GTGAATGGAC TCTTCAGGTG GTTTAAGGAT CACTATCAGG ATCCTGTACC AGGCATCACT 
CCCAGTAGCA GTAACAGAAC ACGGACCCCT GCTTCTATCA ATGCTACCCC AGCCAACATC 
AATCTTGCAG ATCTCACACG GGCTGTGAAT GCCTTGCCTC AGAACATGAC TTCACAGATG 
TTCAGTGCCA TTGCTGCTGT GACAGGCCAG GGGCAGAACC CCAATGCTAC CCCAGCCCAG 
TGGGCATCCA GCCAGTATGG CTACGGTGGC AGCGGAGGAG GCAGCAGCGC CTACCATGTG 
TTCCCAACAC CAGCCCAGCA GCCAGTGGCC ACACCACTAA TGACCCCAAG CTACTCCTAT 
ACTACCCCGA GCCAGCCCAT TACCACCCCA CAATACCACC AGCTCCAGGC CAGCACCACC 
CCACAGTCTA CACAGGCCCA GCCCCAGCCT TCCTCCAGCT CCCGACAACG GCAACAGCAG 
CCAAAGTCCA ACAGCCATGC AGCTATCGAC TGGGGCAAGA TGGCCGAGCA GTGGCTACAG 
GAAAAGGAGG CAGAACGACG GAAACAGAAA CAGCGGCTGA CCCCACGACC CTCTCCCAGC 
CCCATGATCG AGAGCACCCC CATGTCCATT GCTGGCGATG CGACCCCACT CCTGGACGAG 
ATGGATCGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532806.1
CDS	699..5510
Translation	MSDDDEDDEEEYGKEEHEKEAIAGEIFQDEEGEEGQEAVEAPMAPPDEEEEDDEESDIDDFIVDDDGQPLKKPKWRKKLPGYTDAALQEAQEIFGVDFDYDEFEKYNEYDEELEEDYEYEDDETEGEIRVRPKKTTKKRVSRRSIFEMYEPSELESSHLTDQDNEIRATDLPERFQLRSIPVKAAEDDELEEEADWIYRNAFATPTISLQDSCDYLDRGQPTSSFSRKGPSTVQKIKEALGFMRNQHFEVPFIAFYRKEYVEPELHINDLWRVWQWDEKWTQLRIRKENLTRLFEKMQAYQYEQISADPDKPLADGIRALDTTDMERLKDVQSMDELKDVYNHFLLYYGRDIPKMQNAAKASRKKLKRIKEDGDEEGEGEEAEDEEQRGPELKQASRRDMYTICQSAGLDGLAKKFGLTPEQFGENLRDSYQRHETEQFPAEPLELAKDYVCSQFPTPEAVLEGARYMVALQIAREPLVRQVLRQTFQERAKLNITPTKKGRKDVDEAHYAYSFKYLKNKPVKELRDDQFLKIGLAEDEGLLTIDISIDMKGVEGYGNDQTYFEEIKQFYYRDEFSHQVQEWNRQRTMAIERALQQFLYVQMAKELKNKLLAEARESVVKACSRKLYNWLRVAPYRPDQQVEEDDDFMDENQGKGIRVLGIAFSSARDHPVFCALVNGEGEVTDFLRLPHFTKRRTAWREEEREKKAQDIETLKKFLVNKKPHVVTIAGENRDAQMLTEDVKRIVHELDQGQQLSSIGVELVDNELAILYMNSKKSEAEFRDYPPVLRQAVSLARRIQDPLIEFAQVCSSDEDILCLKFHPLQEHVVKEELLNALYCEFINRVNEVGVDVNRAIAHPYSQALIQYVCGLGPRKGTHLLKILKQNNTRLESRTQLVTMCHMGPKVFMNCAGFLKIDTASLGDSTDSYIEVLDGSRVHPETYEWARKMAVDALEYDESAEDANPAGALEEILENPERLKDLDLDAFAEELERQGYGDKHITLYDIRAELSCRYKDLRTAYRSPNTEEIFNMLTKETPETFYIGKLIICNVTGIAHRRPQGESYDQAIRNDETGLWQCPFCQQDNFPELSEVWNHFDSGSCPGQAIGVKTRLDNGVTGFIPTKFLSDKVVKRPEERVKVGMTVHCRIMKIDIEKFSADLTCRTSDLMDRNNEWKLPKDTYYDFDAEAADHKQEEDMKRKQQRTTYIKRVIAHPSFHNINFKQAEKMMETMDQGDVIIRPSSKGENHLTVTWKVSAGIYQHVDVREEGKENAFSLGATLWINSEEFEDLDEIVARYVQPMASFARDLLNHKYYQDCSGGDRKKLEELLIKTKKEKPTFIPYFICACKELPGKFLLGYQPRGKPRIEYVTVTPEGFRYRGQIFPTVNGLFRWFKDHYQDPVPGITPSSSNRTRTPASINATPANINLADLTRAVNALPQNMTSQMFSAIAAVTGQGQNPNATPAQWASSQYGYGGSGGGSSAYHVFPTPAQQPVATPLMTPSYSYTTPSQPITTPQYHQLQASTTPQSTQAQPQPSSSSRQRQQQPKSNSHAAIDWGKMAEQWLQEKEAERRKQKQRLTPRPSPSPMIESTPMSIAGDATPLLDEMDR

Target ORF information:

RefSeq Version	XM_006532743.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus suppressor of Ty 6 (Supt6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532743.3

ORF Insert Sequence:

ATGTCTGATG ATGATGAAGA TGATGAAGAG GAATATGGCA AAGAGGAACA CGAGAAGGAG 
GCCATTGCAG GGGAAATCTT CCAAGATGAA GAAGGGGAAG AAGGCCAGGA GGCTGTAGAG 
GCCCCCATGG CCCCTCCTGA CGAGGAGGAG GAAGATGATG AGGAGTCAGA CATTGACGAT 
TTCATTGTGG ATGATGATGG ACAGCCTCTG AAAAAACCCA AATGGCGAAA AAAGCTCCCT 
GGATATACAG ATGCAGCCCT GCAGGAAGCC CAGGAGATCT TCGGTGTGGA CTTTGACTAT 
GATGAATTTG AGAAATACAA TGAATACGAT GAAGAACTGG AAGAAGACTA TGAGTACGAG 
GATGATGAGA CTGAAGGTGA GATCCGAGTG CGCCCCAAGA AGACCACCAA GAAACGTGTG 
AGTCGCAGGA GCATCTTTGA GATGTACGAG CCTAGTGAGC TGGAGAGCAG CCACCTCACA 
GACCAGGACA ATGAAATCCG AGCCACTGAC TTGCCTGAGA GGTTCCAGCT CCGCTCCATC 
CCAGTCAAGG CAGCTGAGGA TGATGAGCTA GAAGAGGAAG CTGACTGGAT CTATAGAAAT 
GCCTTCGCAA CACCGACCAT CTCCCTCCAG GACAGCTGTG ATTACCTAGA CCGTGGGCAG 
CCAACTAGTA GCTTCAGTCG TAAAGGGCCC AGCACAGTTC AGAAGATCAA AGAGGCCCTG 
GGCTTCATGC GGAATCAGCA TTTTGAGGTG CCTTTCATTG CCTTCTACCG AAAGGAGTAT 
GTGGAACCTG AGCTGCACAT CAATGACCTG TGGAGGGTGT GGCAGTGGGA TGAGAAGTGG 
ACTCAGCTGA GAATCCGCAA AGAGAACCTA ACACGGCTGT TTGAGAAGAT GCAGGCTTAT 
CAGTATGAGC AAATCTCTGC TGACCCTGAC AAACCTCTTG CTGACGGCAT CCGGGCTCTA 
GATACTACTG ACATGGAGAG GCTCAAAGAT GTTCAGTCAA TGGATGAACT GAAAGACGTC 
TACAACCATT TCTTACTTTA CTACGGGCGT GACATCCCCA AGATGCAGAA TGCTGCCAAG 
GCTAGCCGGA AGAAGCTGAA ACGCATAAAG GAGGATGGCG ATGAAGAAGG TGAAGGTGAG 
GAGGCTGAAG ATGAGGAACA GAGGGGACCA GAGCTCAAGC AAGCCTCCCG CCGAGACATG 
TATACCATCT GCCAGAGTGC CGGGCTAGAT GGCCTGGCCA AAAAGTTTGG CCTCACACCT 
GAGCAGTTTG GGGAGAACCT CCGTGACAGC TACCAGCGCC ACGAGACAGA GCAGTTCCCT 
GCAGAGCCTT TGGAGCTGGC CAAAGATTAC GTGTGCAGCC AGTTCCCTAC ACCAGAAGCT 
GTGCTTGAAG GTGCCCGATA CATGGTTGCA CTACAGATTG CCAGGGAGCC CCTTGTCAGA 
CAGGTGCTGA GACAAACCTT TCAGGAGAGA GCCAAGCTAA ACATAACCCC AACCAAGAAA 
GGTAGAAAGG ATGTGGATGA GGCCCACTAC GCTTACTCTT TTAAGTACTT AAAGAACAAG 
CCTGTTAAAG AGCTGAGAGA CGACCAGTTC CTCAAGATAG GCCTGGCTGA GGATGAAGGG 
CTGCTCACCA TTGACATCAG CATAGATATG AAGGGAGTGG AAGGGTATGG TAATGACCAG 
ACATATTTTG AGGAGATCAA GCAGTTTTAC TACCGGGATG AGTTCAGCCA CCAAGTACAG 
GAATGGAACC GGCAGCGCAC TATGGCCATT GAGCGAGCTC TGCAGCAGTT CCTCTACGTG 
CAAATGGCCA AAGAACTTAA GAACAAGCTG CTAGCTGAGG CCAGGGAGAG TGTTGTGAAG 
GCCTGTAGCC GAAAGCTCTA CAACTGGTTG AGAGTGGCAC CCTACCGTCC AGATCAGCAG 
GTAGAGGAGG ACGACGACTT TATGGATGAG AACCAGGGGA AGGGCATCCG AGTCCTAGGC 
ATTGCTTTCT CCTCTGCCAG AGATCACCCT GTGTTCTGTG CCCTAGTCAA TGGTGAAGGA 
GAAGTGACTG ATTTCCTCCG CCTGCCCCAT TTTACCAAGC GGCGAACTGC ATGGAGAGAA 
GAGGAGCGGG AAAAGAAGGC TCAAGATATT GAAACCCTAA AGAAGTTTCT TGTGAATAAG 
AAGCCTCATG TGGTCACTAT CGCAGGAGAG AACAGGGATG CCCAGATGCT GACTGAGGAT 
GTGAAGCGGA TAGTGCATGA GCTGGACCAA GGCCAACAGC TGTCTTCTAT TGGGGTGGAG 
CTGGTTGACA ATGAGCTGGC CATTCTGTAC ATGAACAGCA AGAAATCAGA GGCAGAGTTC 
CGGGATTATC CACCAGTTTT GAGACAAGCT GTCTCTCTAG CCCGGCGTAT CCAAGACCCT 
CTGATAGAAT TTGCCCAGGT GTGCAGCTCA GATGAAGACA TCCTGTGTCT CAAGTTTCAT 
CCCTTGCAGG AGCATGTTGT GAAAGAGGAG CTGCTTAACG CCTTGTACTG TGAATTCATC 
AACCGGGTCA ATGAGGTCGG GGTGGATGTC AACCGTGCCA TTGCCCATCC TTACAGCCAG 
GCCTTGATCC AGTATGTCTG TGGCCTGGGA CCCCGAAAAG GGACCCACCT GCTGAAGATT 
TTGAAACAGA ACAACACTCG ACTCGAGAGC CGGACTCAGC TGGTCACCAT GTGTCATATG 
GGTCCCAAAG TCTTCATGAA TTGTGCTGGC TTCCTCAAGA TCGACACTGC TTCTCTGGGG 
GACAGCACTG ACTCATACAT TGAAGTTCTT GATGGCTCTC GAGTCCACCC TGAAACGTAC 
GAGTGGGCCA GGAAGATGGC AGTGGATGCC TTAGAATACG ATGAGTCTGC AGAAGATGCC 
AATCCTGCTG GAGCCCTTGA GGAAATCTTG GAAAACCCAG AGCGGCTGAA GGATTTAGAT 
CTTGATGCCT TTGCAGAAGA ACTAGAGAGA CAGGGCTATG GTGACAAGCA CATCACACTG 
TATGACATCC GAGCAGAGCT CAGTTGTAGG TATAAGGACC TCCGGACAGC CTACCGCTCT 
CCTAACACAG AGGAGATCTT CAATATGTTA ACCAAAGAGA CGCCAGAGAC CTTCTACATT 
GGAAAGCTTA TCATCTGCAA TGTTACCGGC ATTGCTCACA GGCGCCCACA GGGTGAGAGC 
TATGACCAGG CCATCCGAAA TGATGAGACA GGGCTGTGGC AGTGCCCCTT CTGTCAACAA 
GACAACTTCC CTGAGCTAAG CGAGGTCTGG AACCATTTTG ACAGTGGATC ATGCCCTGGT 
CAGGCCATTG GTGTGAAAAC ACGGCTAGAC AATGGCGTCA CCGGCTTCAT CCCTACCAAA 
TTCCTCAGTG ACAAGGTGGT GAAGAGGCCA GAGGAGCGAG TGAAGGTGGG GATGACTGTT 
CACTGCCGGA TCATGAAGAT CGATATTGAA AAGTTCAGTG CAGACCTGAC CTGCCGGACC 
TCAGACCTCA TGGACAGGAA CAATGAGTGG AAACTGCCCA AGGACACCTA CTATGACTTT 
GATGCGGAAG CTGCAGACCA CAAGCAGGAA GAGGATATGA AACGAAAGCA GCAGCGGACC 
ACATACATCA AGCGAGTGAT TGCACACCCG TCCTTCCATA ACATAAATTT CAAGCAAGCA 
GAAAAGATGA TGGAGACCAT GGACCAAGGG GATGTTATCA TTCGGCCCAG CAGTAAAGGT 
GAAAACCATC TGACGGTGAC CTGGAAAGTC AGTGCTGGCA TCTACCAGCA CGTGGACGTG 
CGGGAAGAGG GCAAGGAGAA CGCCTTCAGC CTGGGAGCCA CTCTTTGGAT CAACAGTGAG 
GAATTTGAAG ACTTGGATGA GATTGTTGCC CGCTATGTCC AGCCCATGGC TTCCTTTGCT 
CGAGACCTTC TGAACCACAA ATATTACCAG GACTGTAGTG GTGGGGACCG AAAGAAATTA 
GAGGAGCTGC TCATCAAAAC TAAGAAGGAG AAGCCCACCT TCATCCCTTA CTTCATCTGT 
GCCTGCAAGG AGCTGCCCGG CAAGTTCCTC CTGGGATACC AGCCCCGGGG TAAACCCAGG 
ATAGAATATG TAACAGTGAC TCCTGAAGGA TTCCGGTACC GGGGCCAGAT TTTCCCAACT 
GTGAATGGAC TCTTCAGGTG GTTTAAGGAT CACTATCAGG ATCCTGTACC AGGCATCACT 
CCCAGTAGCA GTAACAGAAC ACGGACCCCT GCTTCTATCA ATGCTACCCC AGCCAACATC 
AATCTTGCAG ATCTCACACG GGCTGTGAAT GCCTTGCCTC AGAACATGAC TTCACAGATG 
TTCAGTGCCA TTGCTGCTGT GACAGGCCAG GGGCAGAACC CCAATGCTAC CCCAGCCCAG 
TGGGCATCCA GCCAGTATGG CTACGGTGGC AGCGGAGGAG GCAGCAGCGC CTACCATGTG 
TTCCCAACAC CAGCCCAGCA GCCAGTGGCC ACACCACTAA TGACCCCAAG CTACTCCTAT 
ACTACCCCGA GCCAGCCCAT TACCACCCCA CAATACCACC AGCTCCAGGC CAGCACCACC 
CCACAGTCTA CACAGGCCCA GCCCCAGCCT TCCTCCAGCT CCCGACAACG GCAACAGCAG 
CCAAAGTCCA ACAGCCATGC AGCTATCGAC TGGGGCAAGA TGGCCGAGCA GTGGCTACAG 
GAAAAGGAGG CAGAACGACG GAAACAGAAA CAGCGGCTGA CCCCACGACC CTCTCCCAGC 
CCCATGATCG AGAGCACCCC CATGTCCATT GCTGGCGATG CGACCCCACT CCTGGACGAG 
ATGGATCGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Supt6 ( NM_009297.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033323.2 Mus musculus SPT6, histone chaperone and transcription elongation factor (Supt6), mRNA.

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CloneID	OMu21312
Clone ID Related Accession (Same CDS sequence)	NM_009297.2
Accession Version	NM_009297.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	transcription elongation factor SPT6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK136094.1, BC072657.1, AK158846.1 and AK052651.1. On Jan 25, 2008 this sequence version replaced NM_009297.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U40375.1, SRR1660815.210579.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGATT TTGTGGAAAG TGAGGCCGAA GAGTCAGAAG AAGAATACAA TCATGAAGGC 
GAGGTGGTAC CTAGAGTCAC CAAGAAGTTT GTGGAAGAGG AGGATGATGA TGAGGAGGAG 
GAAGAGGAGA ATCTAGATGA TCAAGATGAG CGAGGCAACC TCAAAGACTT TATCAATGAC 
GATGATGATG AAGAAGAAGG GGAGGAGGAT GAGGGCAGCG ACTCTGGGGA CTCAGAAGAC 
GATGTTGGCC ACAAGAAGAG AAAACGCCCA TCTTTTGATG ACCGCCTGGA AGATGATGAT 
TTTGACCTCA TTGAGGAGAA TTTGGGTGTT AAAGTCAAAC GAGGACAAAA ATACCGACGT 
GTCAAAAAAA TGTCTGATGA TGATGAAGAT GATGAAGAGG AATATGGCAA AGAGGAACAC 
GAGAAGGAGG CCATTGCAGG GGAAATCTTC CAAGATGAAG AAGGGGAAGA AGGCCAGGAG 
GCTGTAGAGG CCCCCATGGC CCCTCCTGAC GAGGAGGAGG AAGATGATGA GGAGTCAGAC 
ATTGACGATT TCATTGTGGA TGATGATGGA CAGCCTCTGA AAAAACCCAA ATGGCGAAAA 
AAGCTCCCTG GATATACAGA TGCAGCCCTG CAGGAAGCCC AGGAGATCTT CGGTGTGGAC 
TTTGACTATG ATGAATTTGA GAAATACAAT GAATACGATG AAGAACTGGA AGAAGACTAT 
GAGTACGAGG ATGATGAGAC TGAAGGTGAG ATCCGAGTGC GCCCCAAGAA GACCACCAAG 
AAACGTGTGA GTCGCAGGAG CATCTTTGAG ATGTACGAGC CTAGTGAGCT GGAGAGCAGC 
CACCTCACAG ACCAGGACAA TGAAATCCGA GCCACTGACT TGCCTGAGAG GTTCCAGCTC 
CGCTCCATCC CAGTCAAGGC AGCTGAGGAT GATGAGCTAG AAGAGGAAGC TGACTGGATC 
TATAGAAATG CCTTCGCAAC ACCGACCATC TCCCTCCAGG ACAGCTGTGA TTACCTAGAC 
CGTGGGCAGC CAACTAGTAG CTTCAGTCGT AAAGGGCCCA GCACAGTTCA GAAGATCAAA 
GAGGCCCTGG GCTTCATGCG GAATCAGCAT TTTGAGGTGC CTTTCATTGC CTTCTACCGA 
AAGGAGTATG TGGAACCTGA GCTGCACATC AATGACCTGT GGAGGGTGTG GCAGTGGGAT 
GAGAAGTGGA CTCAGCTGAG AATCCGCAAA GAGAACCTAA CACGGCTGTT TGAGAAGATG 
CAGGCTTATC AGTATGAGCA AATCTCTGCT GACCCTGACA AACCTCTTGC TGACGGCATC 
CGGGCTCTAG ATACTACTGA CATGGAGAGG CTCAAAGATG TTCAGTCAAT GGATGAACTG 
AAAGACGTCT ACAACCATTT CTTACTTTAC TACGGGCGTG ACATCCCCAA GATGCAGAAT 
GCTGCCAAGG CTAGCCGGAA GAAGCTGAAA CGCATAAAGG AGGATGGCGA TGAAGAAGGT 
GAAGGTGAGG AGGCTGAAGA TGAGGAACAG AGGGGACCAG AGCTCAAGCA AGCCTCCCGC 
CGAGACATGT ATACCATCTG CCAGAGTGCC GGGCTAGATG GCCTGGCCAA AAAGTTTGGC 
CTCACACCTG AGCAGTTTGG GGAGAACCTC CGTGACAGCT ACCAGCGCCA CGAGACAGAG 
CAGTTCCCTG CAGAGCCTTT GGAGCTGGCC AAAGATTACG TGTGCAGCCA GTTCCCTACA 
CCAGAAGCTG TGCTTGAAGG TGCCCGATAC ATGGTTGCAC TACAGATTGC CAGGGAGCCC 
CTTGTCAGAC AGGTGCTGAG ACAAACCTTT CAGGAGAGAG CCAAGCTAAA CATAACCCCA 
ACCAAGAAAG GTAGAAAGGA TGTGGATGAG GCCCACTACG CTTACTCTTT TAAGTACTTA 
AAGAACAAGC CTGTTAAAGA GCTGAGAGAC GACCAGTTCC TCAAGATAGG CCTGGCTGAG 
GATGAAGGGC TGCTCACCAT TGACATCAGC ATAGATATGA AGGGAGTGGA AGGGTATGGT 
AATGACCAGA CATATTTTGA GGAGATCAAG CAGTTTTACT ACCGGGATGA GTTCAGCCAC 
CAAGTACAGG AATGGAACCG GCAGCGCACT ATGGCCATTG AGCGAGCTCT GCAGCAGTTC 
CTCTACGTGC AAATGGCCAA AGAACTTAAG AACAAGCTGC TAGCTGAGGC CAGGGAGAGT 
GTTGTGAAGG CCTGTAGCCG AAAGCTCTAC AACTGGTTGA GAGTGGCACC CTACCGTCCA 
GATCAGCAGG TAGAGGAGGA CGACGACTTT ATGGATGAGA ACCAGGGGAA GGGCATCCGA 
GTCCTAGGCA TTGCTTTCTC CTCTGCCAGA GATCACCCTG TGTTCTGTGC CCTAGTCAAT 
GGTGAAGGAG AAGTGACTGA TTTCCTCCGC CTGCCCCATT TTACCAAGCG GCGAACTGCA 
TGGAGAGAAG AGGAGCGGGA AAAGAAGGCT CAAGATATTG AAACCCTAAA GAAGTTTCTT 
GTGAATAAGA AGCCTCATGT GGTCACTATC GCAGGAGAGA ACAGGGATGC CCAGATGCTG 
ACTGAGGATG TGAAGCGGAT AGTGCATGAG CTGGACCAAG GCCAACAGCT GTCTTCTATT 
GGGGTGGAGC TGGTTGACAA TGAGCTGGCC ATTCTGTACA TGAACAGCAA GAAATCAGAG 
GCAGAGTTCC GGGATTATCC ACCAGTTTTG AGACAAGCTG TCTCTCTAGC CCGGCGTATC 
CAAGACCCTC TGATAGAATT TGCCCAGGTG TGCAGCTCAG ATGAAGACAT CCTGTGTCTC 
AAGTTTCATC CCTTGCAGGA GCATGTTGTG AAAGAGGAGC TGCTTAACGC CTTGTACTGT 
GAATTCATCA ACCGGGTCAA TGAGGTCGGG GTGGATGTCA ACCGTGCCAT TGCCCATCCT 
TACAGCCAGG CCTTGATCCA GTATGTCTGT GGCCTGGGAC CCCGAAAAGG GACCCACCTG 
CTGAAGATTT TGAAACAGAA CAACACTCGA CTCGAGAGCC GGACTCAGCT GGTCACCATG 
TGTCATATGG GTCCCAAAGT CTTCATGAAT TGTGCTGGCT TCCTCAAGAT CGACACTGCT 
TCTCTGGGGG ACAGCACTGA CTCATACATT GAAGTTCTTG ATGGCTCTCG AGTCCACCCT 
GAAACGTACG AGTGGGCCAG GAAGATGGCA GTGGATGCCT TAGAATACGA TGAGTCTGCA 
GAAGATGCCA ATCCTGCTGG AGCCCTTGAG GAAATCTTGG AAAACCCAGA GCGGCTGAAG 
GATTTAGATC TTGATGCCTT TGCAGAAGAA CTAGAGAGAC AGGGCTATGG TGACAAGCAC 
ATCACACTGT ATGACATCCG AGCAGAGCTC AGTTGTAGGT ATAAGGACCT CCGGACAGCC 
TACCGCTCTC CTAACACAGA GGAGATCTTC AATATGTTAA CCAAAGAGAC GCCAGAGACC 
TTCTACATTG GAAAGCTTAT CATCTGCAAT GTTACCGGCA TTGCTCACAG GCGCCCACAG 
GGTGAGAGCT ATGACCAGGC CATCCGAAAT GATGAGACAG GGCTGTGGCA GTGCCCCTTC 
TGTCAACAAG ACAACTTCCC TGAGCTAAGC GAGGTCTGGA ACCATTTTGA CAGTGGATCA 
TGCCCTGGTC AGGCCATTGG TGTGAAAACA CGGCTAGACA ATGGCGTCAC CGGCTTCATC 
CCTACCAAAT TCCTCAGTGA CAAGGTGGTG AAGAGGCCAG AGGAGCGAGT GAAGGTGGGG 
ATGACTGTTC ACTGCCGGAT CATGAAGATC GATATTGAAA AGTTCAGTGC AGACCTGACC 
TGCCGGACCT CAGACCTCAT GGACAGGAAC AATGAGTGGA AACTGCCCAA GGACACCTAC 
TATGACTTTG ATGCGGAAGC TGCAGACCAC AAGCAGGAAG AGGATATGAA ACGAAAGCAG 
CAGCGGACCA CATACATCAA GCGAGTGATT GCACACCCGT CCTTCCATAA CATAAATTTC 
AAGCAAGCAG AAAAGATGAT GGAGACCATG GACCAAGGGG ATGTTATCAT TCGGCCCAGC 
AGTAAAGGTG AAAACCATCT GACGGTGACC TGGAAAGTCA GTGCTGGCAT CTACCAGCAC 
GTGGACGTGC GGGAAGAGGG CAAGGAGAAC GCCTTCAGCC TGGGAGCCAC TCTTTGGATC 
AACAGTGAGG AATTTGAAGA CTTGGATGAG ATTGTTGCCC GCTATGTCCA GCCCATGGCT 
TCCTTTGCTC GAGACCTTCT GAACCACAAA TATTACCAGG ACTGTAGTGG TGGGGACCGA 
AAGAAATTAG AGGAGCTGCT CATCAAAACT AAGAAGGAGA AGCCCACCTT CATCCCTTAC 
TTCATCTGTG CCTGCAAGGA GCTGCCCGGC AAGTTCCTCC TGGGATACCA GCCCCGGGGT 
AAACCCAGGA TAGAATATGT AACAGTGACT CCTGAAGGAT TCCGGTACCG GGGCCAGATT 
TTCCCAACTG TGAATGGACT CTTCAGGTGG TTTAAGGATC ACTATCAGGA TCCTGTACCA 
GGCATCACTC CCAGTAGCAG TAACAGAACA CGGACCCCTG CTTCTATCAA TGCTACCCCA 
GCCAACATCA ATCTTGCAGA TCTCACACGG GCTGTGAATG CCTTGCCTCA GAACATGACT 
TCACAGATGT TCAGTGCCAT TGCTGCTGTG ACAGGCCAGG GGCAGAACCC CAATGCTACC 
CCAGCCCAGT GGGCATCCAG CCAGTATGGC TACGGTGGCA GCGGAGGAGG CAGCAGCGCC 
TACCATGTGT TCCCAACACC AGCCCAGCAG CCAGTGGCCA CACCACTAAT GACCCCAAGC 
TACTCCTATA CTACCCCGAG CCAGCCCATT ACCACCCCAC AATACCACCA GCTCCAGGCC 
AGCACCACCC CACAGTCTAC ACAGGCCCAG CCCCAGCCTT CCTCCAGCTC CCGACAACGG 
CAACAGCAGC CAAAGTCCAA CAGCCATGCA GCTATCGACT GGGGCAAGAT GGCCGAGCAG 
TGGCTACAGG AAAAGGAGGC AGAACGACGG AAACAGAAAC AGCGGCTGAC CCCACGACCC 
TCTCCCAGCC CCATGATCGA GAGCACCCCC ATGTCCATTG CTGGCGATGC GACCCCACTC 
CTGGACGAGA TGGATCGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033323.2
CDS	187..5367
Translation	MSDFVESEAEESEEEYNHEGEVVPRVTKKFVEEEDDDEEEEEENLDDQDERGNLKDFINDDDDEEEGEEDEGSDSGDSEDDVGHKKRKRPSFDDRLEDDDFDLIEENLGVKVKRGQKYRRVKKMSDDDEDDEEEYGKEEHEKEAIAGEIFQDEEGEEGQEAVEAPMAPPDEEEEDDEESDIDDFIVDDDGQPLKKPKWRKKLPGYTDAALQEAQEIFGVDFDYDEFEKYNEYDEELEEDYEYEDDETEGEIRVRPKKTTKKRVSRRSIFEMYEPSELESSHLTDQDNEIRATDLPERFQLRSIPVKAAEDDELEEEADWIYRNAFATPTISLQDSCDYLDRGQPTSSFSRKGPSTVQKIKEALGFMRNQHFEVPFIAFYRKEYVEPELHINDLWRVWQWDEKWTQLRIRKENLTRLFEKMQAYQYEQISADPDKPLADGIRALDTTDMERLKDVQSMDELKDVYNHFLLYYGRDIPKMQNAAKASRKKLKRIKEDGDEEGEGEEAEDEEQRGPELKQASRRDMYTICQSAGLDGLAKKFGLTPEQFGENLRDSYQRHETEQFPAEPLELAKDYVCSQFPTPEAVLEGARYMVALQIAREPLVRQVLRQTFQERAKLNITPTKKGRKDVDEAHYAYSFKYLKNKPVKELRDDQFLKIGLAEDEGLLTIDISIDMKGVEGYGNDQTYFEEIKQFYYRDEFSHQVQEWNRQRTMAIERALQQFLYVQMAKELKNKLLAEARESVVKACSRKLYNWLRVAPYRPDQQVEEDDDFMDENQGKGIRVLGIAFSSARDHPVFCALVNGEGEVTDFLRLPHFTKRRTAWREEEREKKAQDIETLKKFLVNKKPHVVTIAGENRDAQMLTEDVKRIVHELDQGQQLSSIGVELVDNELAILYMNSKKSEAEFRDYPPVLRQAVSLARRIQDPLIEFAQVCSSDEDILCLKFHPLQEHVVKEELLNALYCEFINRVNEVGVDVNRAIAHPYSQALIQYVCGLGPRKGTHLLKILKQNNTRLESRTQLVTMCHMGPKVFMNCAGFLKIDTASLGDSTDSYIEVLDGSRVHPETYEWARKMAVDALEYDESAEDANPAGALEEILENPERLKDLDLDAFAEELERQGYGDKHITLYDIRAELSCRYKDLRTAYRSPNTEEIFNMLTKETPETFYIGKLIICNVTGIAHRRPQGESYDQAIRNDETGLWQCPFCQQDNFPELSEVWNHFDSGSCPGQAIGVKTRLDNGVTGFIPTKFLSDKVVKRPEERVKVGMTVHCRIMKIDIEKFSADLTCRTSDLMDRNNEWKLPKDTYYDFDAEAADHKQEEDMKRKQQRTTYIKRVIAHPSFHNINFKQAEKMMETMDQGDVIIRPSSKGENHLTVTWKVSAGIYQHVDVREEGKENAFSLGATLWINSEEFEDLDEIVARYVQPMASFARDLLNHKYYQDCSGGDRKKLEELLIKTKKEKPTFIPYFICACKELPGKFLLGYQPRGKPRIEYVTVTPEGFRYRGQIFPTVNGLFRWFKDHYQDPVPGITPSSSNRTRTPASINATPANINLADLTRAVNALPQNMTSQMFSAIAAVTGQGQNPNATPAQWASSQYGYGGSGGGSSAYHVFPTPAQQPVATPLMTPSYSYTTPSQPITTPQYHQLQASTTPQSTQAQPQPSSSSRQRQQQPKSNSHAAIDWGKMAEQWLQEKEAERRKQKQRLTPRPSPSPMIESTPMSIAGDATPLLDEMDR

Target ORF information:

RefSeq Version	NM_009297.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPT6, histone chaperone and transcription elongation factor (Supt6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009297.2

ORF Insert Sequence:

ATGTCTGATT TTGTGGAAAG TGAGGCCGAA GAGTCAGAAG AAGAATACAA TCATGAAGGC 
GAGGTGGTAC CTAGAGTCAC CAAGAAGTTT GTGGAAGAGG AGGATGATGA TGAGGAGGAG 
GAAGAGGAGA ATCTAGATGA TCAAGATGAG CGAGGCAACC TCAAAGACTT TATCAATGAC 
GATGATGATG AAGAAGAAGG GGAGGAGGAT GAGGGCAGCG ACTCTGGGGA CTCAGAAGAC 
GATGTTGGCC ACAAGAAGAG AAAACGCCCA TCTTTTGATG ACCGCCTGGA AGATGATGAT 
TTTGACCTCA TTGAGGAGAA TTTGGGTGTT AAAGTCAAAC GAGGACAAAA ATACCGACGT 
GTCAAAAAAA TGTCTGATGA TGATGAAGAT GATGAAGAGG AATATGGCAA AGAGGAACAC 
GAGAAGGAGG CCATTGCAGG GGAAATCTTC CAAGATGAAG AAGGGGAAGA AGGCCAGGAG 
GCTGTAGAGG CCCCCATGGC CCCTCCTGAC GAGGAGGAGG AAGATGATGA GGAGTCAGAC 
ATTGACGATT TCATTGTGGA TGATGATGGA CAGCCTCTGA AAAAACCCAA ATGGCGAAAA 
AAGCTCCCTG GATATACAGA TGCAGCCCTG CAGGAAGCCC AGGAGATCTT CGGTGTGGAC 
TTTGACTATG ATGAATTTGA GAAATACAAT GAATACGATG AAGAACTGGA AGAAGACTAT 
GAGTACGAGG ATGATGAGAC TGAAGGTGAG ATCCGAGTGC GCCCCAAGAA GACCACCAAG 
AAACGTGTGA GTCGCAGGAG CATCTTTGAG ATGTACGAGC CTAGTGAGCT GGAGAGCAGC 
CACCTCACAG ACCAGGACAA TGAAATCCGA GCCACTGACT TGCCTGAGAG GTTCCAGCTC 
CGCTCCATCC CAGTCAAGGC AGCTGAGGAT GATGAGCTAG AAGAGGAAGC TGACTGGATC 
TATAGAAATG CCTTCGCAAC ACCGACCATC TCCCTCCAGG ACAGCTGTGA TTACCTAGAC 
CGTGGGCAGC CAACTAGTAG CTTCAGTCGT AAAGGGCCCA GCACAGTTCA GAAGATCAAA 
GAGGCCCTGG GCTTCATGCG GAATCAGCAT TTTGAGGTGC CTTTCATTGC CTTCTACCGA 
AAGGAGTATG TGGAACCTGA GCTGCACATC AATGACCTGT GGAGGGTGTG GCAGTGGGAT 
GAGAAGTGGA CTCAGCTGAG AATCCGCAAA GAGAACCTAA CACGGCTGTT TGAGAAGATG 
CAGGCTTATC AGTATGAGCA AATCTCTGCT GACCCTGACA AACCTCTTGC TGACGGCATC 
CGGGCTCTAG ATACTACTGA CATGGAGAGG CTCAAAGATG TTCAGTCAAT GGATGAACTG 
AAAGACGTCT ACAACCATTT CTTACTTTAC TACGGGCGTG ACATCCCCAA GATGCAGAAT 
GCTGCCAAGG CTAGCCGGAA GAAGCTGAAA CGCATAAAGG AGGATGGCGA TGAAGAAGGT 
GAAGGTGAGG AGGCTGAAGA TGAGGAACAG AGGGGACCAG AGCTCAAGCA AGCCTCCCGC 
CGAGACATGT ATACCATCTG CCAGAGTGCC GGGCTAGATG GCCTGGCCAA AAAGTTTGGC 
CTCACACCTG AGCAGTTTGG GGAGAACCTC CGTGACAGCT ACCAGCGCCA CGAGACAGAG 
CAGTTCCCTG CAGAGCCTTT GGAGCTGGCC AAAGATTACG TGTGCAGCCA GTTCCCTACA 
CCAGAAGCTG TGCTTGAAGG TGCCCGATAC ATGGTTGCAC TACAGATTGC CAGGGAGCCC 
CTTGTCAGAC AGGTGCTGAG ACAAACCTTT CAGGAGAGAG CCAAGCTAAA CATAACCCCA 
ACCAAGAAAG GTAGAAAGGA TGTGGATGAG GCCCACTACG CTTACTCTTT TAAGTACTTA 
AAGAACAAGC CTGTTAAAGA GCTGAGAGAC GACCAGTTCC TCAAGATAGG CCTGGCTGAG 
GATGAAGGGC TGCTCACCAT TGACATCAGC ATAGATATGA AGGGAGTGGA AGGGTATGGT 
AATGACCAGA CATATTTTGA GGAGATCAAG CAGTTTTACT ACCGGGATGA GTTCAGCCAC 
CAAGTACAGG AATGGAACCG GCAGCGCACT ATGGCCATTG AGCGAGCTCT GCAGCAGTTC 
CTCTACGTGC AAATGGCCAA AGAACTTAAG AACAAGCTGC TAGCTGAGGC CAGGGAGAGT 
GTTGTGAAGG CCTGTAGCCG AAAGCTCTAC AACTGGTTGA GAGTGGCACC CTACCGTCCA 
GATCAGCAGG TAGAGGAGGA CGACGACTTT ATGGATGAGA ACCAGGGGAA GGGCATCCGA 
GTCCTAGGCA TTGCTTTCTC CTCTGCCAGA GATCACCCTG TGTTCTGTGC CCTAGTCAAT 
GGTGAAGGAG AAGTGACTGA TTTCCTCCGC CTGCCCCATT TTACCAAGCG GCGAACTGCA 
TGGAGAGAAG AGGAGCGGGA AAAGAAGGCT CAAGATATTG AAACCCTAAA GAAGTTTCTT 
GTGAATAAGA AGCCTCATGT GGTCACTATC GCAGGAGAGA ACAGGGATGC CCAGATGCTG 
ACTGAGGATG TGAAGCGGAT AGTGCATGAG CTGGACCAAG GCCAACAGCT GTCTTCTATT 
GGGGTGGAGC TGGTTGACAA TGAGCTGGCC ATTCTGTACA TGAACAGCAA GAAATCAGAG 
GCAGAGTTCC GGGATTATCC ACCAGTTTTG AGACAAGCTG TCTCTCTAGC CCGGCGTATC 
CAAGACCCTC TGATAGAATT TGCCCAGGTG TGCAGCTCAG ATGAAGACAT CCTGTGTCTC 
AAGTTTCATC CCTTGCAGGA GCATGTTGTG AAAGAGGAGC TGCTTAACGC CTTGTACTGT 
GAATTCATCA ACCGGGTCAA TGAGGTCGGG GTGGATGTCA ACCGTGCCAT TGCCCATCCT 
TACAGCCAGG CCTTGATCCA GTATGTCTGT GGCCTGGGAC CCCGAAAAGG GACCCACCTG 
CTGAAGATTT TGAAACAGAA CAACACTCGA CTCGAGAGCC GGACTCAGCT GGTCACCATG 
TGTCATATGG GTCCCAAAGT CTTCATGAAT TGTGCTGGCT TCCTCAAGAT CGACACTGCT 
TCTCTGGGGG ACAGCACTGA CTCATACATT GAAGTTCTTG ATGGCTCTCG AGTCCACCCT 
GAAACGTACG AGTGGGCCAG GAAGATGGCA GTGGATGCCT TAGAATACGA TGAGTCTGCA 
GAAGATGCCA ATCCTGCTGG AGCCCTTGAG GAAATCTTGG AAAACCCAGA GCGGCTGAAG 
GATTTAGATC TTGATGCCTT TGCAGAAGAA CTAGAGAGAC AGGGCTATGG TGACAAGCAC 
ATCACACTGT ATGACATCCG AGCAGAGCTC AGTTGTAGGT ATAAGGACCT CCGGACAGCC 
TACCGCTCTC CTAACACAGA GGAGATCTTC AATATGTTAA CCAAAGAGAC GCCAGAGACC 
TTCTACATTG GAAAGCTTAT CATCTGCAAT GTTACCGGCA TTGCTCACAG GCGCCCACAG 
GGTGAGAGCT ATGACCAGGC CATCCGAAAT GATGAGACAG GGCTGTGGCA GTGCCCCTTC 
TGTCAACAAG ACAACTTCCC TGAGCTAAGC GAGGTCTGGA ACCATTTTGA CAGTGGATCA 
TGCCCTGGTC AGGCCATTGG TGTGAAAACA CGGCTAGACA ATGGCGTCAC CGGCTTCATC 
CCTACCAAAT TCCTCAGTGA CAAGGTGGTG AAGAGGCCAG AGGAGCGAGT GAAGGTGGGG 
ATGACTGTTC ACTGCCGGAT CATGAAGATC GATATTGAAA AGTTCAGTGC AGACCTGACC 
TGCCGGACCT CAGACCTCAT GGACAGGAAC AATGAGTGGA AACTGCCCAA GGACACCTAC 
TATGACTTTG ATGCGGAAGC TGCAGACCAC AAGCAGGAAG AGGATATGAA ACGAAAGCAG 
CAGCGGACCA CATACATCAA GCGAGTGATT GCACACCCGT CCTTCCATAA CATAAATTTC 
AAGCAAGCAG AAAAGATGAT GGAGACCATG GACCAAGGGG ATGTTATCAT TCGGCCCAGC 
AGTAAAGGTG AAAACCATCT GACGGTGACC TGGAAAGTCA GTGCTGGCAT CTACCAGCAC 
GTGGACGTGC GGGAAGAGGG CAAGGAGAAC GCCTTCAGCC TGGGAGCCAC TCTTTGGATC 
AACAGTGAGG AATTTGAAGA CTTGGATGAG ATTGTTGCCC GCTATGTCCA GCCCATGGCT 
TCCTTTGCTC GAGACCTTCT GAACCACAAA TATTACCAGG ACTGTAGTGG TGGGGACCGA 
AAGAAATTAG AGGAGCTGCT CATCAAAACT AAGAAGGAGA AGCCCACCTT CATCCCTTAC 
TTCATCTGTG CCTGCAAGGA GCTGCCCGGC AAGTTCCTCC TGGGATACCA GCCCCGGGGT 
AAACCCAGGA TAGAATATGT AACAGTGACT CCTGAAGGAT TCCGGTACCG GGGCCAGATT 
TTCCCAACTG TGAATGGACT CTTCAGGTGG TTTAAGGATC ACTATCAGGA TCCTGTACCA 
GGCATCACTC CCAGTAGCAG TAACAGAACA CGGACCCCTG CTTCTATCAA TGCTACCCCA 
GCCAACATCA ATCTTGCAGA TCTCACACGG GCTGTGAATG CCTTGCCTCA GAACATGACT 
TCACAGATGT TCAGTGCCAT TGCTGCTGTG ACAGGCCAGG GGCAGAACCC CAATGCTACC 
CCAGCCCAGT GGGCATCCAG CCAGTATGGC TACGGTGGCA GCGGAGGAGG CAGCAGCGCC 
TACCATGTGT TCCCAACACC AGCCCAGCAG CCAGTGGCCA CACCACTAAT GACCCCAAGC 
TACTCCTATA CTACCCCGAG CCAGCCCATT ACCACCCCAC AATACCACCA GCTCCAGGCC 
AGCACCACCC CACAGTCTAC ACAGGCCCAG CCCCAGCCTT CCTCCAGCTC CCGACAACGG 
CAACAGCAGC CAAAGTCCAA CAGCCATGCA GCTATCGACT GGGGCAAGAT GGCCGAGCAG 
TGGCTACAGG AAAAGGAGGC AGAACGACGG AAACAGAAAC AGCGGCTGAC CCCACGACCC 
TCTCCCAGCC CCATGATCGA GAGCACCCCC ATGTCCATTG CTGGCGATGC GACCCCACTC 
CTGGACGAGA TGGATCGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Elongation Factor Spt6 Maintains ESC Pluripotency by Controlling Super-Enhancers and Counteracting Polycomb Proteins.
Molecular cell68(2)398-413.e6(2017 Oct)
Wang AH,Juan AH,Ko KD,Tsai PF,Zare H,Dell'Orso S,Sartorelli V

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Supt6 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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