Nhsl1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006512688.3	XP_006512751.1	NHS-like protein 1 isoform X3
XM_030245006.1	XP_030100866.1	NHS-like protein 1 isoform X9
NM_173390.3	NP_775566.3	NHS-like protein 1 isoform 1
XM_006512686.3	XP_006512749.1	NHS-like protein 1 isoform X1
XM_006512687.3	XP_006512750.1	NHS-like protein 1 isoform X2
XM_006512686.4	XP_006512749.1	NHS-like protein 1 isoform X1
XM_030245005.1	XP_030100865.1	NHS-like protein 1 isoform X8
XM_030245008.1	XP_030100868.1	NHS-like protein 1 isoform X9
NM_001163592.1	NP_001157064.1	NHS-like protein 1 isoform 2
XM_006512689.3	XP_006512752.1	NHS-like protein 1 isoform X4
XM_006512689.4	XP_006512752.1	NHS-like protein 1 isoform X4
XM_030245004.1	XP_030100864.1	NHS-like protein 1 isoform X7
XM_006512688.4	XP_006512751.1	NHS-like protein 1 isoform X3
XM_006512690.4	XP_006512753.1	NHS-like protein 1 isoform X5
XM_030245003.1	XP_030100863.1	NHS-like protein 1 isoform X6
XM_030245009.1	XP_030100869.1	NHS-like protein 1 isoform X9
XM_006512690.3	XP_006512753.1	NHS-like protein 1 isoform X5
XM_006512691.3	XP_006512754.1	NHS-like protein 1 isoform X6
XM_006512687.4	XP_006512750.1	NHS-like protein 1 isoform X2
XM_006512691.4	XP_006512754.1	NHS-like protein 1 isoform X9
XM_030245007.1	XP_030100867.1	NHS-like protein 1 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu43848	XM_030245009.1 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu43848	XM_030245008.1 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu43848	XM_006512691.4 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu43848	XM_030245007.1 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu43848	XM_030245006.1 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu43843	XM_006512686.4 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu43844	XM_006512687.4 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu43845	XM_006512688.4 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu43846	XM_006512689.4 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu43847	XM_006512690.4 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85408	XM_030245003.1 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85409	XM_030245004.1 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu85410	XM_030245005.1 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu04541	NM_173390.3 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$937.30 $1339.00
OMu09232	NM_001163592.1 Latest version!	Mus musculus NHS-like 1 (Nhsl1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$930.30 $1329.00
View Old Accession Versions >>
OMu43843	XM_006512686.3	Mus musculus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu43846	XM_006512689.3	Mus musculus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu43845	XM_006512688.3	Mus musculus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu43847	XM_006512690.3	Mus musculus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu43844	XM_006512687.3	Mus musculus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu43848	XM_006512691.3	Mus musculus NHS-like 1 (Nhsl1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

Nhsl1 ( XM_030245006.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100866.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu43848
Clone ID Related Accession (Same CDS sequence)	XM_030245006.1 , XM_030245008.1 , XM_030245009.1 , XM_006512691.3 , XM_006512691.4 , XM_030245007.1
Accession Version	XM_030245006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC 
CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT 
TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC 
TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT 
ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT 
GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC 
TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA 
CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT 
GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA 
CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG 
GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT 
AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT 
TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT 
GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT 
AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT 
TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC 
AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG 
AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG 
AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC 
AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG 
CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC 
AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT 
AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC 
CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC 
TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG 
GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC 
CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC 
ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG 
CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA 
CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC 
TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC 
TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC 
AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG 
CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC 
ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG 
CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT 
CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA 
TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG 
CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA 
GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT 
CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG 
GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG 
AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA 
GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG 
AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA 
GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT 
ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA 
GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA 
GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA 
CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG 
AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC 
TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT 
AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT 
GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA 
CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC 
ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT 
CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC 
TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC 
CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG 
AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC 
AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA 
GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG 
GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC 
GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG 
TGTGGGGGTA CGGAACAGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100866.1
CDS	873..4799
Translation	MRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_030245006.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245006.1

ORF Insert Sequence:

ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC 
CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT 
TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC 
TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT 
ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT 
GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC 
TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA 
CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT 
GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA 
CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG 
GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT 
AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT 
TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT 
GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT 
AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT 
TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC 
AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG 
AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG 
AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC 
AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG 
CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC 
AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT 
AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC 
CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC 
TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG 
GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC 
CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC 
ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG 
CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA 
CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC 
TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC 
TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC 
AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG 
CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC 
ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG 
CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT 
CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA 
TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG 
CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA 
GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT 
CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG 
GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG 
AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA 
GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG 
AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA 
GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT 
ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA 
GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA 
GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA 
CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG 
AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC 
TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT 
AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT 
GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA 
CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC 
ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT 
CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC 
TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC 
CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG 
AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC 
AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA 
GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG 
GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC 
GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG 
TGTGGGGGTA CGGAACAGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_030245008.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100868.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu43848
Clone ID Related Accession (Same CDS sequence)	XM_030245006.1 , XM_030245008.1 , XM_030245009.1 , XM_006512691.3 , XM_006512691.4 , XM_030245007.1
Accession Version	XM_030245008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC 
CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT 
TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC 
TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT 
ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT 
GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC 
TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA 
CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT 
GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA 
CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG 
GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT 
AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT 
TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT 
GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT 
AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT 
TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC 
AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG 
AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG 
AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC 
AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG 
CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC 
AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT 
AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC 
CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC 
TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG 
GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC 
CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC 
ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG 
CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA 
CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC 
TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC 
TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC 
AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG 
CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC 
ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG 
CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT 
CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA 
TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG 
CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA 
GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT 
CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG 
GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG 
AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA 
GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG 
AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA 
GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT 
ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA 
GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA 
GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA 
CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG 
AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC 
TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT 
AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT 
GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA 
CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC 
ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT 
CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC 
TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC 
CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG 
AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC 
AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA 
GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG 
GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC 
GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG 
TGTGGGGGTA CGGAACAGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100868.1
CDS	805..4731
Translation	MRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_030245008.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245008.1

ORF Insert Sequence:

ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC 
CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT 
TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC 
TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT 
ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT 
GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC 
TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA 
CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT 
GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA 
CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG 
GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT 
AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT 
TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT 
GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT 
AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT 
TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC 
AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG 
AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG 
AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC 
AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG 
CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC 
AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT 
AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC 
CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC 
TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG 
GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC 
CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC 
ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG 
CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA 
CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC 
TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC 
TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC 
AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG 
CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC 
ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG 
CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT 
CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA 
TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG 
CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA 
GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT 
CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG 
GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG 
AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA 
GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG 
AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA 
GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT 
ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA 
GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA 
GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA 
CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG 
AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC 
TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT 
AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT 
GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA 
CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC 
ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT 
CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC 
TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC 
CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG 
AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC 
AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA 
GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG 
GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC 
GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG 
TGTGGGGGTA CGGAACAGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_030245009.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100869.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X13, mRNA.

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CloneID	OMu43848
Clone ID Related Accession (Same CDS sequence)	XM_030245006.1 , XM_030245008.1 , XM_030245009.1 , XM_006512691.3 , XM_006512691.4 , XM_030245007.1
Accession Version	XM_030245009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC 
CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT 
TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC 
TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT 
ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT 
GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC 
TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA 
CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT 
GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA 
CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG 
GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT 
AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT 
TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT 
GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT 
AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT 
TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC 
AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG 
AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG 
AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC 
AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG 
CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC 
AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT 
AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC 
CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC 
TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG 
GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC 
CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC 
ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG 
CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA 
CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC 
TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC 
TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC 
AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG 
CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC 
ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG 
CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT 
CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA 
TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG 
CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA 
GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT 
CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG 
GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG 
AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA 
GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG 
AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA 
GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT 
ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA 
GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA 
GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA 
CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG 
AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC 
TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT 
AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT 
GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA 
CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC 
ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT 
CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC 
TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC 
CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG 
AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC 
AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA 
GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG 
GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC 
GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG 
TGTGGGGGTA CGGAACAGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100869.1
CDS	917..4843
Translation	MRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_030245009.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245009.1

ORF Insert Sequence:

ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC 
CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT 
TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC 
TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT 
ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT 
GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC 
TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA 
CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT 
GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA 
CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG 
GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT 
AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT 
TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT 
GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT 
AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT 
TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC 
AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG 
AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG 
AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC 
AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG 
CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC 
AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT 
AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC 
CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC 
TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG 
GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC 
CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC 
ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG 
CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA 
CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC 
TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC 
TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC 
AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG 
CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC 
ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG 
CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT 
CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA 
TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG 
CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA 
GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT 
CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG 
GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG 
AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA 
GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG 
AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA 
GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT 
ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA 
GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA 
GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA 
CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG 
AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC 
TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT 
AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT 
GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA 
CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC 
ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT 
CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC 
TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC 
CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG 
AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC 
AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA 
GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG 
GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC 
GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG 
TGTGGGGGTA CGGAACAGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006512691.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512754.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu43848
Clone ID Related Accession (Same CDS sequence)	XM_030245006.1 , XM_030245008.1 , XM_030245009.1 , XM_006512691.3 , XM_006512691.4 , XM_030245007.1
Accession Version	XM_006512691.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512691.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC 
CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT 
TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC 
TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT 
ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT 
GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC 
TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA 
CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT 
GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA 
CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG 
GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT 
AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT 
TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT 
GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT 
AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT 
TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC 
AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG 
AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG 
AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC 
AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG 
CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC 
AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT 
AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC 
CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC 
TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG 
GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC 
CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC 
ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG 
CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA 
CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC 
TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC 
TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC 
AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG 
CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC 
ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG 
CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT 
CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA 
TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG 
CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA 
GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT 
CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG 
GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG 
AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA 
GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG 
AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA 
GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT 
ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA 
GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA 
GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA 
CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG 
AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC 
TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT 
AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT 
GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA 
CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC 
ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT 
CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC 
TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC 
CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG 
AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC 
AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA 
GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG 
GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC 
GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG 
TGTGGGGGTA CGGAACAGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512754.1
CDS	13489..17415
Translation	MRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_006512691.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512691.4

ORF Insert Sequence:

ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC 
CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT 
TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC 
TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT 
ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT 
GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC 
TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA 
CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT 
GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA 
CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG 
GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT 
AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT 
TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT 
GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT 
AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT 
TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC 
AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG 
AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG 
AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC 
AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG 
CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC 
AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT 
AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC 
CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC 
TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG 
GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC 
CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC 
ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG 
CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA 
CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC 
TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC 
TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC 
AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG 
CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC 
ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG 
CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT 
CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA 
TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG 
CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA 
GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT 
CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG 
GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG 
AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA 
GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG 
AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA 
GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT 
ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA 
GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA 
GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA 
CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG 
AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC 
TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT 
AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT 
GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA 
CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC 
ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT 
CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC 
TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC 
CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG 
AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC 
AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA 
GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG 
GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC 
GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG 
TGTGGGGGTA CGGAACAGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_030245007.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100867.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu43848
Clone ID Related Accession (Same CDS sequence)	XM_030245006.1 , XM_030245008.1 , XM_030245009.1 , XM_006512691.3 , XM_006512691.4 , XM_030245007.1
Accession Version	XM_030245007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC 
CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT 
TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC 
TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT 
ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT 
GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC 
TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA 
CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT 
GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA 
CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG 
GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT 
AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT 
TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT 
GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT 
AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT 
TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC 
AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG 
AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG 
AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC 
AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG 
CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC 
AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT 
AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC 
CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC 
TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG 
GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC 
CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC 
ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG 
CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA 
CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC 
TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC 
TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC 
AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG 
CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC 
ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG 
CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT 
CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA 
TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG 
CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA 
GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT 
CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG 
GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG 
AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA 
GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG 
AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA 
GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT 
ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA 
GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA 
GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA 
CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG 
AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC 
TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT 
AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT 
GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA 
CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC 
ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT 
CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC 
TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC 
CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG 
AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC 
AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA 
GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG 
GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC 
GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG 
TGTGGGGGTA CGGAACAGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100867.1
CDS	1306..5232
Translation	MRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_030245007.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245007.1

ORF Insert Sequence:

ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC 
CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT 
TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC 
TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT 
ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT 
GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC 
TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA 
CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT 
GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA 
CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG 
GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT 
AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT 
TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT 
GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT 
AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT 
TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC 
AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG 
AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG 
AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC 
AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG 
CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC 
AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT 
AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC 
CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC 
TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG 
GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC 
CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC 
ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG 
CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA 
CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC 
TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC 
TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC 
AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG 
CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC 
ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG 
CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT 
CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA 
TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG 
CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA 
GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT 
CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG 
GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG 
AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA 
GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG 
AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA 
GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT 
ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA 
GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA 
GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA 
CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG 
AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC 
TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT 
AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT 
GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA 
CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC 
ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT 
CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC 
TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC 
CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG 
AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC 
AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA 
GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG 
GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC 
GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG 
TGTGGGGGTA CGGAACAGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006512691.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512754.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X6, mRNA.

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CloneID	OMu43848
Clone ID Related Accession (Same CDS sequence)	XM_030245006.1 , XM_030245008.1 , XM_030245009.1 , XM_006512691.3 , XM_006512691.4 , XM_030245007.1
Accession Version	XM_006512691.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512691.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC 
CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT 
TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC 
TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT 
ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT 
GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC 
TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA 
CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT 
GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA 
CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG 
GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT 
AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT 
TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT 
GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT 
AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT 
TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC 
AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG 
AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG 
AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC 
AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG 
CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC 
AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT 
AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC 
CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC 
TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG 
GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC 
CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC 
ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG 
CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA 
CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC 
TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC 
TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC 
AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG 
CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC 
ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG 
CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT 
CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA 
TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG 
CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA 
GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT 
CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG 
GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG 
AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA 
GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG 
AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA 
GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT 
ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA 
GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA 
GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA 
CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG 
AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC 
TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT 
AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT 
GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA 
CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC 
ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT 
CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC 
TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC 
CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG 
AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC 
AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA 
GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG 
GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC 
GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG 
TGTGGGGGTA CGGAACAGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512754.1
CDS	1476..5402
Translation	MRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_006512691.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512691.3

ORF Insert Sequence:

ATGAGAAGGA TCAGGGCTCA CAAGGGGGTA GGCGTTGCTG CCCAGATGAG CCACCTCTCA 
GGTTCCTCAG GCAACATGTC TGTGCTGAGT GACTCAGCCG GTGTTGTGTT CCCATCTCGC 
CTCAGTAACG ATACTGGTTT CCACAGTCTT CCACGTACCG GCCCGAGAGC AAGCACCTAT 
TCACTGGAGG GAAGGATGGG TGCCCTGGGC TCTACTGAAG ATACGGATGA CACTTCTCCC 
TACCAGGGGG GTAGCTTACA GGGGCATGAA AACTTTGCGC ATTTAGGAGG TGCGTCGAGT 
ACTGGGATGC TTTCGAGGCC CAAATCCCAG CAGTTGAGGT TTTTGGAGAG TCCAGCATGT 
GTGGTTTCAC CTCATGCAGC CTACTCTACC AGTGTCATCC CAAATGCCAC GCTACTGTCC 
TCTTCAGAGG TTATTGTCAT TCACACTGCT CAGAGTGCAG GACAGCTGGA CAGTAGAACA 
CCCGGTTCAT CTTCATATTC AAAAATAAAA CCCAGAGACC GTCCCACACC CAGATGTTCT 
GTGAAAGATG ACCATCAGTC CCCCCGTCAT CACTGGAACG AGGGTCATCT CATTCATTCA 
CGGGCTTTAG CCTCCTCTGT CCCTGGTGCC ACCACGCTGT TATCCCTCCA TGATTCAGAG 
GTCTCTCTAA ATGCCCCAGC AAACAGGGAA AATGGATCCC AAGCCATACT CTATCATTGT 
AGAAACAACC CAAGCTTTCC TGACCACCCT TCAGATGTGG ATGGCAAGAG CGAGTGTAGT 
TATTCGGGGG ACAGGGGATG TGGCAGTTCT GAGCCCTGGG AATATAAAAC CTCCAGCAAT 
GGGCGGGCAT CCCCACTGAA GCCACACCTG GCCACTCCTG GTTGCTCTAC TCCCACAAGT 
AACGTGAGCA GCTGCAGTTT GGACCAAACA TCTCTCAAGG GTGATACCAG GTCCCTGTGT 
TCAGAGGACC ATGATGGTTA CTATACTACC ACTCACGAGG CTGGGAACCT GTACACTCTC 
AGTGATGGCT TGGGGAACCC CAGGCACAGC ATGGTCAATG TTTTTGATGG AAGGGCTCAG 
AGAAGCCAAG GGGATCAGGC CGCTCACCAG GATAAAATCC TTTCGAGAAA CATCTCTCTG 
AAGAAAGCAA AGAAACCACC CCTGCCACCA TCCCGGACCG ATTCTCTGCG AAGGATTCCC 
AAAAAGAACA ACCAGACAAA CGGTCAGGTG CTCAATGAGA GCCTGATCGC CTCGCTCCAG 
CATTCACTGC AGCTAAGCCT CCCTGGCAAG GGCGGCAGCT CCCCTTCCCA GAGCCCCTGC 
AGTGACTTTG AGGAACCCTG GCTCCCCAGG TCCAGAAGCC AGAGCATTGT TAGCGAGGGT 
AGTAGCTTGA CCTCCACCAC CACTCCCAAC GTGTATTCTC TGTGTGGGGT CACTCCGTCC 
CAGAGCGACA CAAGTAGCGT CAAGTCGGAA TACACGGACC CTTGGGGTTA CTACATTGAC 
TACACGAGCT TGCAGGAAGA TCCAGGGAAC CCCACCGGGG GCTGCTCAGC CAACACTGAG 
GCAGCAACTG GAAACGGGCC AGTACGCCAC ATCCAGGAGG GGTCGAGAGT CCCAGTGCCC 
CAAGTGCCGG GCTGCTCAGT TAGACCAAAG ATCGCCTCAC CAGAGAAGTC ACAGAGAGTC 
ACCTCTCCAT CCAGTGGGTA TTCTAGCCAG TCGAATACAC CCACAGCACT CACCCCTGTG 
CCTGTGTTTT TAAAATCAAT GTCACCAGCA AATGGAAAGG GGAAGGCCAA GCCCAAGGTA 
CCAGAAAGAA AATCGTCTCT GATCTCTTCT ATGTCCATCT CATCATCGTC CACTTCTCTC 
TCTTCTAATA CTTCTACCGA AGGAAGTGGG ACCATGAAGA AACTGGATAC AACCCTGGCC 
TCGGCCCTTG CCCCTCCCCC TCCTCCTCTT CCCCCTCTGC CTTCTCCGTG TCTGGCAGAC 
AAGTCCCCTT TTCTTCCACC ACCTCCTCCT CTAGCAGATT GCTCCGAGGG CTCTCCTCTG 
CCGCCCTCTC CCATGTTCCC CCCTCCACCA CCAGAAGCCC TTGTTCCCTT CTGCTCCCCC 
ACCGACGGGT GCCTTTCTCC TTCTCCCACA GCAGTTAGCC CCTCTCTTCC AAGATCCTTG 
CCTCCTGTGC CAGCACCTCC ACCTTTCTTG CCCTCATCAG AACCCCCGCC CGCTCCGCCT 
CTGGACCCCA AACTCATGAA AGAAAACAGG CCCTTTTTCA AAAACTCTAG CCAGTCCGAA 
TCCTCTAGGG AGGCCCTCAG GCGGCCTGCC AACAAGGAGG AGGGCTGCCG ACCCCCCATG 
CCCCTGATCA CCACAGAAGC TTTGCAGATG GTGCAGTTGA GGCCAGTGAG GAAGAACTCA 
GGTGCCGGGG CAGTCCTGTT TTCTGAACCA TCAGCTCAGG AACAACGAAC TCCGACTGCT 
CCACAGTATC ACTTAAAGCC ATCTGCTTTC CTCAAATCCC GAAATAGCAT AAATGAAATG 
GAGAGTGAAA GCCAGGCTGC CTCTGTGACG AGCTCGCTTC CGATGCCTGC CAAGAGCCAG 
AGTCAGGGTG ACCATGACAG TGCAGTAGAG CGTGGCGGCC TTCCGAGCTG CAGCGATGGA 
GCTCCAGGCC CGGGTCCCAG CCTCAGGACC ACTCTGCTCC CAGACTCTTC ACCCAGCAGG 
AAGCCACCCC CCATCTCTAA GAAGCCCAAA CTGTTCCTGG TGGTACCACC TCCGCAGAGA 
GATTTCACAG CGGAGCCCAC AGAGAACGGG AGCGAAGCTT TCCCAGGCGT GCCCAGCCCT 
ACCAGGGCAG AGGGGGAAGC TGTGCGGAGC CAAGAGGAGA AATCCAGCCC AGCATCCCGA 
GCTGGCTCTC ATGCTACGGC CCCCACCCCC GGCAGTCCGG CTCTAGAACC GGGAACTGCA 
GGGTCCTTAT CCTCTAGCAT TGTGGAAGCC AATGTCCCCA TGGTCCAACC TAATACATCA 
CCGGGCCCCA CACAGGAAGA GTCAGGCGAG AACAGTGTGG ATGGTGAGAG AAATGCGAAG 
AGCTGCCTGT CTCAACAGGG CAGAGAAGCT GGCTTGCTGG AACCCAACAC AGCTGCTTCC 
TCTTCAGACC CCGTGGATGT ATCTAAGGAA GAGGGAAGCG ATGAGGTGCT GACTCCGACT 
AAACCCAGGA CAACAGAAGA CCTGTTTGCA GCCATTCACA GATCTAAAAG GAAAGTCCTT 
GGCCGGAAAG ACTCAGAAGA CGATCACACT CGAAATCACT CTCCCTCCCC GCCTGTGACA 
CCCACGAGTG CTGCCCCTAA CCTGGCCTCT CCAAAGCAAG TAGGGTCTAT CCAGAGAAGC 
ATAAAAAAGA GCACTACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTAAA GAAGGGGAGT 
CGCTCGGACA CCAGCGCTCG CATGTCAGCG GCTGAGATGC TCAAGTCTAC GGACCCTCGC 
TTCCAGAGAT CTAGGTCGGA GCCTTCGGCA GACAGCCCGG ACAGCCCATC CAGCTGCTCC 
CCCAACAAGA ACAGGAGAGC TCAGGAAGAG TGGGCCAAGA ACGAAGGCCT GATGCCTCGG 
AGTCTGTCCT TTTCGGGCCC TCGGTACAGC CGCAGCAGGA CGCCACCCTC TGCGGCCAGC 
AGCAGGTACA GCATGCGGAA CCGCATCCAG AGCAGCCCCA TGACCGTCAT CTCCGAGGGA 
GAGGGAGAAC CTGCTGAGCC AGCGGACAAC AAAGCACGCA GGGCCCTGGA TGCTACCAGG 
GTGTGCTCTC TGGACAGACT GACGGGTCAG GAGATGGACC AGGCCAGCCT CCTCTGTAGC 
GAGGAGCCTG CCTCTGTGGA TGGGATAGGA AGGGCTGAGG GCAACGGTCC CTCGGAGCAG 
TGTGGGGGTA CGGAACAGAA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006512686.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512749.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.

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CloneID	OMu43843
Clone ID Related Accession (Same CDS sequence)	XM_006512686.3 , XM_006512686.4
Accession Version	XM_006512686.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512686.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG 
AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT 
CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA 
GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGGGGAGAA TTTCGATCGT 
CAGGCCAGCC TTCGGCGGTC TCTAATTTAC ACAGACACTC TGGTAAGACG ACCGAAGAAA 
GTCAAAAGGA GAAAGACAAT TTCAGGAATC CCTGACATCA TTCAGAAGGA GCTAGCATCA 
GCCACTGGCC AAGATGATGA TGGTAGTGCC CATTCGCTGT ACGTCCCAGA CCACTACTCT 
ACGTTAGGAA GGCTTGATAG TTATCGGTCC ACCGGGCAAT GCTTGGAAAC CAGGGACACC 
AGCTGTCAGA CCGAAGACGT GAAGGTCATC CCACCATCAA TGAGAAGGAT CAGGGCTCAC 
AAGGGGGTAG GCGTTGCTGC CCAGATGAGC CACCTCTCAG GTTCCTCAGG CAACATGTCT 
GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC CCATCTCGCC TCAGTAACGA TACTGGTTTC 
CACAGTCTTC CACGTACCGG CCCGAGAGCA AGCACCTATT CACTGGAGGG AAGGATGGGT 
GCCCTGGGCT CTACTGAAGA TACGGATGAC ACTTCTCCCT ACCAGGGGGG TAGCTTACAG 
GGGCATGAAA ACTTTGCGCA TTTAGGAGGT GCGTCGAGTA CTGGGATGCT TTCGAGGCCC 
AAATCCCAGC AGTTGAGGTT TTTGGAGAGT CCAGCATGTG TGGTTTCACC TCATGCAGCC 
TACTCTACCA GTGTCATCCC AAATGCCACG CTACTGTCCT CTTCAGAGGT TATTGTCATT 
CACACTGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAACAC CCGGTTCATC TTCATATTCA 
AAAATAAAAC CCAGAGACCG TCCCACACCC AGATGTTCTG TGAAAGATGA CCATCAGTCC 
CCCCGTCATC ACTGGAACGA GGGTCATCTC ATTCATTCAC GGGCTTTAGC CTCCTCTGTC 
CCTGGTGCCA CCACGCTGTT ATCCCTCCAT GATTCAGAGG TCTCTCTAAA TGCCCCAGCA 
AACAGGGAAA ATGGATCCCA AGCCATACTC TATCATTGTA GAAACAACCC AAGCTTTCCT 
GACCACCCTT CAGATGTGGA TGGCAAGAGC GAGTGTAGTT ATTCGGGGGA CAGGGGATGT 
GGCAGTTCTG AGCCCTGGGA ATATAAAACC TCCAGCAATG GGCGGGCATC CCCACTGAAG 
CCACACCTGG CCACTCCTGG TTGCTCTACT CCCACAAGTA ACGTGAGCAG CTGCAGTTTG 
GACCAAACAT CTCTCAAGGG TGATACCAGG TCCCTGTGTT CAGAGGACCA TGATGGTTAC 
TATACTACCA CTCACGAGGC TGGGAACCTG TACACTCTCA GTGATGGCTT GGGGAACCCC 
AGGCACAGCA TGGTCAATGT TTTTGATGGA AGGGCTCAGA GAAGCCAAGG GGATCAGGCC 
GCTCACCAGG ATAAAATCCT TTCGAGAAAC ATCTCTCTGA AGAAAGCAAA GAAACCACCC 
CTGCCACCAT CCCGGACCGA TTCTCTGCGA AGGATTCCCA AAAAGAACAA CCAGACAAAC 
GGTCAGGTGC TCAATGAGAG CCTGATCGCC TCGCTCCAGC ATTCACTGCA GCTAAGCCTC 
CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG AGCCCCTGCA GTGACTTTGA GGAACCCTGG 
CTCCCCAGGT CCAGAAGCCA GAGCATTGTT AGCGAGGGTA GTAGCTTGAC CTCCACCACC 
ACTCCCAACG TGTATTCTCT GTGTGGGGTC ACTCCGTCCC AGAGCGACAC AAGTAGCGTC 
AAGTCGGAAT ACACGGACCC TTGGGGTTAC TACATTGACT ACACGAGCTT GCAGGAAGAT 
CCAGGGAACC CCACCGGGGG CTGCTCAGCC AACACTGAGG CAGCAACTGG AAACGGGCCA 
GTACGCCACA TCCAGGAGGG GTCGAGAGTC CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT 
AGACCAAAGA TCGCCTCACC AGAGAAGTCA CAGAGAGTCA CCTCTCCATC CAGTGGGTAT 
TCTAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAATG 
TCACCAGCAA ATGGAAAGGG GAAGGCCAAG CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG 
ATCTCTTCTA TGTCCATCTC ATCATCGTCC ACTTCTCTCT CTTCTAATAC TTCTACCGAA 
GGAAGTGGGA CCATGAAGAA ACTGGATACA ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT 
CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT CTGGCAGACA AGTCCCCTTT TCTTCCACCA 
CCTCCTCCTC TAGCAGATTG CTCCGAGGGC TCTCCTCTGC CGCCCTCTCC CATGTTCCCC 
CCTCCACCAC CAGAAGCCCT TGTTCCCTTC TGCTCCCCCA CCGACGGGTG CCTTTCTCCT 
TCTCCCACAG CAGTTAGCCC CTCTCTTCCA AGATCCTTGC CTCCTGTGCC AGCACCTCCA 
CCTTTCTTGC CCTCATCAGA ACCCCCGCCC GCTCCGCCTC TGGACCCCAA ACTCATGAAA 
GAAAACAGGC CCTTTTTCAA AAACTCTAGC CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG 
CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA CCCCCCATGC CCCTGATCAC CACAGAAGCT 
TTGCAGATGG TGCAGTTGAG GCCAGTGAGG AAGAACTCAG GTGCCGGGGC AGTCCTGTTT 
TCTGAACCAT CAGCTCAGGA ACAACGAACT CCGACTGCTC CACAGTATCA CTTAAAGCCA 
TCTGCTTTCC TCAAATCCCG AAATAGCATA AATGAAATGG AGAGTGAAAG CCAGGCTGCC 
TCTGTGACGA GCTCGCTTCC GATGCCTGCC AAGAGCCAGA GTCAGGGTGA CCATGACAGT 
GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC AGCGATGGAG CTCCAGGCCC GGGTCCCAGC 
CTCAGGACCA CTCTGCTCCC AGACTCTTCA CCCAGCAGGA AGCCACCCCC CATCTCTAAG 
AAGCCCAAAC TGTTCCTGGT GGTACCACCT CCGCAGAGAG ATTTCACAGC GGAGCCCACA 
GAGAACGGGA GCGAAGCTTT CCCAGGCGTG CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT 
GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA GCATCCCGAG CTGGCTCTCA TGCTACGGCC 
CCCACCCCCG GCAGTCCGGC TCTAGAACCG GGAACTGCAG GGTCCTTATC CTCTAGCATT 
GTGGAAGCCA ATGTCCCCAT GGTCCAACCT AATACATCAC CGGGCCCCAC ACAGGAAGAG 
TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA AATGCGAAGA GCTGCCTGTC TCAACAGGGC 
AGAGAAGCTG GCTTGCTGGA ACCCAACACA GCTGCTTCCT CTTCAGACCC CGTGGATGTA 
TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG ACTCCGACTA AACCCAGGAC AACAGAAGAC 
CTGTTTGCAG CCATTCACAG ATCTAAAAGG AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC 
GATCACACTC GAAATCACTC TCCCTCCCCG CCTGTGACAC CCACGAGTGC TGCCCCTAAC 
CTGGCCTCTC CAAAGCAAGT AGGGTCTATC CAGAGAAGCA TAAAAAAGAG CACTACCAGC 
AGTGACAACT TCAAAGCTCT GCTGCTAAAG AAGGGGAGTC GCTCGGACAC CAGCGCTCGC 
ATGTCAGCGG CTGAGATGCT CAAGTCTACG GACCCTCGCT TCCAGAGATC TAGGTCGGAG 
CCTTCGGCAG ACAGCCCGGA CAGCCCATCC AGCTGCTCCC CCAACAAGAA CAGGAGAGCT 
CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT 
CGGTACAGCC GCAGCAGGAC GCCACCCTCT GCGGCCAGCA GCAGGTACAG CATGCGGAAC 
CGCATCCAGA GCAGCCCCAT GACCGTCATC TCCGAGGGAG AGGGAGAACC TGCTGAGCCA 
GCGGACAACA AAGCACGCAG GGCCCTGGAT GCTACCAGGG TGTGCTCTCT GGACAGACTG 
ACGGGTCAGG AGATGGACCA GGCCAGCCTC CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT 
GGGATAGGAA GGGCTGAGGG CAACGGTCCC TCGGAGCAGT GTGGGGGTAC GGAACAGAAG 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512749.1
CDS	486..5471
Translation	MPFHQRSVEPARLRRPDEAALARAGDPPLFRSLEQVSSHTLVCLLAQLADLSRCAGDIFGELEGQAAALGHRTAELHRRLDALHAAAARLDHRRVKIPVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_006512686.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512686.4

ORF Insert Sequence:

ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG 
AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT 
CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA 
GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGGGGAGAA TTTCGATCGT 
CAGGCCAGCC TTCGGCGGTC TCTAATTTAC ACAGACACTC TGGTAAGACG ACCGAAGAAA 
GTCAAAAGGA GAAAGACAAT TTCAGGAATC CCTGACATCA TTCAGAAGGA GCTAGCATCA 
GCCACTGGCC AAGATGATGA TGGTAGTGCC CATTCGCTGT ACGTCCCAGA CCACTACTCT 
ACGTTAGGAA GGCTTGATAG TTATCGGTCC ACCGGGCAAT GCTTGGAAAC CAGGGACACC 
AGCTGTCAGA CCGAAGACGT GAAGGTCATC CCACCATCAA TGAGAAGGAT CAGGGCTCAC 
AAGGGGGTAG GCGTTGCTGC CCAGATGAGC CACCTCTCAG GTTCCTCAGG CAACATGTCT 
GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC CCATCTCGCC TCAGTAACGA TACTGGTTTC 
CACAGTCTTC CACGTACCGG CCCGAGAGCA AGCACCTATT CACTGGAGGG AAGGATGGGT 
GCCCTGGGCT CTACTGAAGA TACGGATGAC ACTTCTCCCT ACCAGGGGGG TAGCTTACAG 
GGGCATGAAA ACTTTGCGCA TTTAGGAGGT GCGTCGAGTA CTGGGATGCT TTCGAGGCCC 
AAATCCCAGC AGTTGAGGTT TTTGGAGAGT CCAGCATGTG TGGTTTCACC TCATGCAGCC 
TACTCTACCA GTGTCATCCC AAATGCCACG CTACTGTCCT CTTCAGAGGT TATTGTCATT 
CACACTGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAACAC CCGGTTCATC TTCATATTCA 
AAAATAAAAC CCAGAGACCG TCCCACACCC AGATGTTCTG TGAAAGATGA CCATCAGTCC 
CCCCGTCATC ACTGGAACGA GGGTCATCTC ATTCATTCAC GGGCTTTAGC CTCCTCTGTC 
CCTGGTGCCA CCACGCTGTT ATCCCTCCAT GATTCAGAGG TCTCTCTAAA TGCCCCAGCA 
AACAGGGAAA ATGGATCCCA AGCCATACTC TATCATTGTA GAAACAACCC AAGCTTTCCT 
GACCACCCTT CAGATGTGGA TGGCAAGAGC GAGTGTAGTT ATTCGGGGGA CAGGGGATGT 
GGCAGTTCTG AGCCCTGGGA ATATAAAACC TCCAGCAATG GGCGGGCATC CCCACTGAAG 
CCACACCTGG CCACTCCTGG TTGCTCTACT CCCACAAGTA ACGTGAGCAG CTGCAGTTTG 
GACCAAACAT CTCTCAAGGG TGATACCAGG TCCCTGTGTT CAGAGGACCA TGATGGTTAC 
TATACTACCA CTCACGAGGC TGGGAACCTG TACACTCTCA GTGATGGCTT GGGGAACCCC 
AGGCACAGCA TGGTCAATGT TTTTGATGGA AGGGCTCAGA GAAGCCAAGG GGATCAGGCC 
GCTCACCAGG ATAAAATCCT TTCGAGAAAC ATCTCTCTGA AGAAAGCAAA GAAACCACCC 
CTGCCACCAT CCCGGACCGA TTCTCTGCGA AGGATTCCCA AAAAGAACAA CCAGACAAAC 
GGTCAGGTGC TCAATGAGAG CCTGATCGCC TCGCTCCAGC ATTCACTGCA GCTAAGCCTC 
CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG AGCCCCTGCA GTGACTTTGA GGAACCCTGG 
CTCCCCAGGT CCAGAAGCCA GAGCATTGTT AGCGAGGGTA GTAGCTTGAC CTCCACCACC 
ACTCCCAACG TGTATTCTCT GTGTGGGGTC ACTCCGTCCC AGAGCGACAC AAGTAGCGTC 
AAGTCGGAAT ACACGGACCC TTGGGGTTAC TACATTGACT ACACGAGCTT GCAGGAAGAT 
CCAGGGAACC CCACCGGGGG CTGCTCAGCC AACACTGAGG CAGCAACTGG AAACGGGCCA 
GTACGCCACA TCCAGGAGGG GTCGAGAGTC CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT 
AGACCAAAGA TCGCCTCACC AGAGAAGTCA CAGAGAGTCA CCTCTCCATC CAGTGGGTAT 
TCTAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAATG 
TCACCAGCAA ATGGAAAGGG GAAGGCCAAG CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG 
ATCTCTTCTA TGTCCATCTC ATCATCGTCC ACTTCTCTCT CTTCTAATAC TTCTACCGAA 
GGAAGTGGGA CCATGAAGAA ACTGGATACA ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT 
CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT CTGGCAGACA AGTCCCCTTT TCTTCCACCA 
CCTCCTCCTC TAGCAGATTG CTCCGAGGGC TCTCCTCTGC CGCCCTCTCC CATGTTCCCC 
CCTCCACCAC CAGAAGCCCT TGTTCCCTTC TGCTCCCCCA CCGACGGGTG CCTTTCTCCT 
TCTCCCACAG CAGTTAGCCC CTCTCTTCCA AGATCCTTGC CTCCTGTGCC AGCACCTCCA 
CCTTTCTTGC CCTCATCAGA ACCCCCGCCC GCTCCGCCTC TGGACCCCAA ACTCATGAAA 
GAAAACAGGC CCTTTTTCAA AAACTCTAGC CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG 
CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA CCCCCCATGC CCCTGATCAC CACAGAAGCT 
TTGCAGATGG TGCAGTTGAG GCCAGTGAGG AAGAACTCAG GTGCCGGGGC AGTCCTGTTT 
TCTGAACCAT CAGCTCAGGA ACAACGAACT CCGACTGCTC CACAGTATCA CTTAAAGCCA 
TCTGCTTTCC TCAAATCCCG AAATAGCATA AATGAAATGG AGAGTGAAAG CCAGGCTGCC 
TCTGTGACGA GCTCGCTTCC GATGCCTGCC AAGAGCCAGA GTCAGGGTGA CCATGACAGT 
GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC AGCGATGGAG CTCCAGGCCC GGGTCCCAGC 
CTCAGGACCA CTCTGCTCCC AGACTCTTCA CCCAGCAGGA AGCCACCCCC CATCTCTAAG 
AAGCCCAAAC TGTTCCTGGT GGTACCACCT CCGCAGAGAG ATTTCACAGC GGAGCCCACA 
GAGAACGGGA GCGAAGCTTT CCCAGGCGTG CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT 
GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA GCATCCCGAG CTGGCTCTCA TGCTACGGCC 
CCCACCCCCG GCAGTCCGGC TCTAGAACCG GGAACTGCAG GGTCCTTATC CTCTAGCATT 
GTGGAAGCCA ATGTCCCCAT GGTCCAACCT AATACATCAC CGGGCCCCAC ACAGGAAGAG 
TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA AATGCGAAGA GCTGCCTGTC TCAACAGGGC 
AGAGAAGCTG GCTTGCTGGA ACCCAACACA GCTGCTTCCT CTTCAGACCC CGTGGATGTA 
TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG ACTCCGACTA AACCCAGGAC AACAGAAGAC 
CTGTTTGCAG CCATTCACAG ATCTAAAAGG AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC 
GATCACACTC GAAATCACTC TCCCTCCCCG CCTGTGACAC CCACGAGTGC TGCCCCTAAC 
CTGGCCTCTC CAAAGCAAGT AGGGTCTATC CAGAGAAGCA TAAAAAAGAG CACTACCAGC 
AGTGACAACT TCAAAGCTCT GCTGCTAAAG AAGGGGAGTC GCTCGGACAC CAGCGCTCGC 
ATGTCAGCGG CTGAGATGCT CAAGTCTACG GACCCTCGCT TCCAGAGATC TAGGTCGGAG 
CCTTCGGCAG ACAGCCCGGA CAGCCCATCC AGCTGCTCCC CCAACAAGAA CAGGAGAGCT 
CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT 
CGGTACAGCC GCAGCAGGAC GCCACCCTCT GCGGCCAGCA GCAGGTACAG CATGCGGAAC 
CGCATCCAGA GCAGCCCCAT GACCGTCATC TCCGAGGGAG AGGGAGAACC TGCTGAGCCA 
GCGGACAACA AAGCACGCAG GGCCCTGGAT GCTACCAGGG TGTGCTCTCT GGACAGACTG 
ACGGGTCAGG AGATGGACCA GGCCAGCCTC CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT 
GGGATAGGAA GGGCTGAGGG CAACGGTCCC TCGGAGCAGT GTGGGGGTAC GGAACAGAAG 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006512687.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512750.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.

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CloneID	OMu43844
Clone ID Related Accession (Same CDS sequence)	XM_006512687.3 , XM_006512687.4
Accession Version	XM_006512687.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512687.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG ACGGCTCCTC AGGTTCCTTC GGAATCAAGG CAAGCCCAGG CTCCCTCTCA 
CGTGCTGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTCCGC 
AGTGATGGAG AGCTGTCCGT TTGTGGGCAC CAGGTGGAAG CCGATGATGA GAACTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTGGACG AGGAGAGTCG ATGGACTGTC 
CACTACACGG CACCATGGCA TCAGCAAGAG AATGTGTTCC TTCCCGCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCAAGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCGGCT CCAGCCCCTG CGACGATTAT CAAGATGAAG ACACAGAAGC AGACCGAAAG 
TGCTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATCGGAA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCCGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC GCGGCAGCAA GCCCAGACGG TCCAGGCTGA CGTGGTTCCT 
ATAAACATAA CTGGGGAGAA TTTCGATCGT CAGGCCAGCC TTCGGCGGTC TCTAATTTAC 
ACAGACACTC TGGTAAGACG ACCGAAGAAA GTCAAAAGGA GAAAGACAAT TTCAGGAATC 
CCTGACATCA TTCAGAAGGA GCTAGCATCA GCCACTGGCC AAGATGATGA TGGTAGTGCC 
CATTCGCTGT ACGTCCCAGA CCACTACTCT ACGTTAGGAA GGCTTGATAG TTATCGGTCC 
ACCGGGCAAT GCTTGGAAAC CAGGGACACC AGCTGTCAGA CCGAAGACGT GAAGGTCATC 
CCACCATCAA TGAGAAGGAT CAGGGCTCAC AAGGGGGTAG GCGTTGCTGC CCAGATGAGC 
CACCTCTCAG GTTCCTCAGG CAACATGTCT GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC 
CCATCTCGCC TCAGTAACGA TACTGGTTTC CACAGTCTTC CACGTACCGG CCCGAGAGCA 
AGCACCTATT CACTGGAGGG AAGGATGGGT GCCCTGGGCT CTACTGAAGA TACGGATGAC 
ACTTCTCCCT ACCAGGGGGG TAGCTTACAG GGGCATGAAA ACTTTGCGCA TTTAGGAGGT 
GCGTCGAGTA CTGGGATGCT TTCGAGGCCC AAATCCCAGC AGTTGAGGTT TTTGGAGAGT 
CCAGCATGTG TGGTTTCACC TCATGCAGCC TACTCTACCA GTGTCATCCC AAATGCCACG 
CTACTGTCCT CTTCAGAGGT TATTGTCATT CACACTGCTC AGAGTGCAGG ACAGCTGGAC 
AGTAGAACAC CCGGTTCATC TTCATATTCA AAAATAAAAC CCAGAGACCG TCCCACACCC 
AGATGTTCTG TGAAAGATGA CCATCAGTCC CCCCGTCATC ACTGGAACGA GGGTCATCTC 
ATTCATTCAC GGGCTTTAGC CTCCTCTGTC CCTGGTGCCA CCACGCTGTT ATCCCTCCAT 
GATTCAGAGG TCTCTCTAAA TGCCCCAGCA AACAGGGAAA ATGGATCCCA AGCCATACTC 
TATCATTGTA GAAACAACCC AAGCTTTCCT GACCACCCTT CAGATGTGGA TGGCAAGAGC 
GAGTGTAGTT ATTCGGGGGA CAGGGGATGT GGCAGTTCTG AGCCCTGGGA ATATAAAACC 
TCCAGCAATG GGCGGGCATC CCCACTGAAG CCACACCTGG CCACTCCTGG TTGCTCTACT 
CCCACAAGTA ACGTGAGCAG CTGCAGTTTG GACCAAACAT CTCTCAAGGG TGATACCAGG 
TCCCTGTGTT CAGAGGACCA TGATGGTTAC TATACTACCA CTCACGAGGC TGGGAACCTG 
TACACTCTCA GTGATGGCTT GGGGAACCCC AGGCACAGCA TGGTCAATGT TTTTGATGGA 
AGGGCTCAGA GAAGCCAAGG GGATCAGGCC GCTCACCAGG ATAAAATCCT TTCGAGAAAC 
ATCTCTCTGA AGAAAGCAAA GAAACCACCC CTGCCACCAT CCCGGACCGA TTCTCTGCGA 
AGGATTCCCA AAAAGAACAA CCAGACAAAC GGTCAGGTGC TCAATGAGAG CCTGATCGCC 
TCGCTCCAGC ATTCACTGCA GCTAAGCCTC CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG 
AGCCCCTGCA GTGACTTTGA GGAACCCTGG CTCCCCAGGT CCAGAAGCCA GAGCATTGTT 
AGCGAGGGTA GTAGCTTGAC CTCCACCACC ACTCCCAACG TGTATTCTCT GTGTGGGGTC 
ACTCCGTCCC AGAGCGACAC AAGTAGCGTC AAGTCGGAAT ACACGGACCC TTGGGGTTAC 
TACATTGACT ACACGAGCTT GCAGGAAGAT CCAGGGAACC CCACCGGGGG CTGCTCAGCC 
AACACTGAGG CAGCAACTGG AAACGGGCCA GTACGCCACA TCCAGGAGGG GTCGAGAGTC 
CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT AGACCAAAGA TCGCCTCACC AGAGAAGTCA 
CAGAGAGTCA CCTCTCCATC CAGTGGGTAT TCTAGCCAGT CGAATACACC CACAGCACTC 
ACCCCTGTGC CTGTGTTTTT AAAATCAATG TCACCAGCAA ATGGAAAGGG GAAGGCCAAG 
CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG ATCTCTTCTA TGTCCATCTC ATCATCGTCC 
ACTTCTCTCT CTTCTAATAC TTCTACCGAA GGAAGTGGGA CCATGAAGAA ACTGGATACA 
ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT 
CTGGCAGACA AGTCCCCTTT TCTTCCACCA CCTCCTCCTC TAGCAGATTG CTCCGAGGGC 
TCTCCTCTGC CGCCCTCTCC CATGTTCCCC CCTCCACCAC CAGAAGCCCT TGTTCCCTTC 
TGCTCCCCCA CCGACGGGTG CCTTTCTCCT TCTCCCACAG CAGTTAGCCC CTCTCTTCCA 
AGATCCTTGC CTCCTGTGCC AGCACCTCCA CCTTTCTTGC CCTCATCAGA ACCCCCGCCC 
GCTCCGCCTC TGGACCCCAA ACTCATGAAA GAAAACAGGC CCTTTTTCAA AAACTCTAGC 
CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA 
CCCCCCATGC CCCTGATCAC CACAGAAGCT TTGCAGATGG TGCAGTTGAG GCCAGTGAGG 
AAGAACTCAG GTGCCGGGGC AGTCCTGTTT TCTGAACCAT CAGCTCAGGA ACAACGAACT 
CCGACTGCTC CACAGTATCA CTTAAAGCCA TCTGCTTTCC TCAAATCCCG AAATAGCATA 
AATGAAATGG AGAGTGAAAG CCAGGCTGCC TCTGTGACGA GCTCGCTTCC GATGCCTGCC 
AAGAGCCAGA GTCAGGGTGA CCATGACAGT GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC 
AGCGATGGAG CTCCAGGCCC GGGTCCCAGC CTCAGGACCA CTCTGCTCCC AGACTCTTCA 
CCCAGCAGGA AGCCACCCCC CATCTCTAAG AAGCCCAAAC TGTTCCTGGT GGTACCACCT 
CCGCAGAGAG ATTTCACAGC GGAGCCCACA GAGAACGGGA GCGAAGCTTT CCCAGGCGTG 
CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA 
GCATCCCGAG CTGGCTCTCA TGCTACGGCC CCCACCCCCG GCAGTCCGGC TCTAGAACCG 
GGAACTGCAG GGTCCTTATC CTCTAGCATT GTGGAAGCCA ATGTCCCCAT GGTCCAACCT 
AATACATCAC CGGGCCCCAC ACAGGAAGAG TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA 
AATGCGAAGA GCTGCCTGTC TCAACAGGGC AGAGAAGCTG GCTTGCTGGA ACCCAACACA 
GCTGCTTCCT CTTCAGACCC CGTGGATGTA TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG 
ACTCCGACTA AACCCAGGAC AACAGAAGAC CTGTTTGCAG CCATTCACAG ATCTAAAAGG 
AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC GATCACACTC GAAATCACTC TCCCTCCCCG 
CCTGTGACAC CCACGAGTGC TGCCCCTAAC CTGGCCTCTC CAAAGCAAGT AGGGTCTATC 
CAGAGAAGCA TAAAAAAGAG CACTACCAGC AGTGACAACT TCAAAGCTCT GCTGCTAAAG 
AAGGGGAGTC GCTCGGACAC CAGCGCTCGC ATGTCAGCGG CTGAGATGCT CAAGTCTACG 
GACCCTCGCT TCCAGAGATC TAGGTCGGAG CCTTCGGCAG ACAGCCCGGA CAGCCCATCC 
AGCTGCTCCC CCAACAAGAA CAGGAGAGCT CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG 
ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT CGGTACAGCC GCAGCAGGAC GCCACCCTCT 
GCGGCCAGCA GCAGGTACAG CATGCGGAAC CGCATCCAGA GCAGCCCCAT GACCGTCATC 
TCCGAGGGAG AGGGAGAACC TGCTGAGCCA GCGGACAACA AAGCACGCAG GGCCCTGGAT 
GCTACCAGGG TGTGCTCTCT GGACAGACTG ACGGGTCAGG AGATGGACCA GGCCAGCCTC 
CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT GGGATAGGAA GGGCTGAGGG CAACGGTCCC 
TCGGAGCAGT GTGGGGGTAC GGAACAGAAG AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512750.1
CDS	857..5752
Translation	MKKDGSSGSFGIKASPGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGHQVEADDENWIYRTQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_006512687.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512687.4

ORF Insert Sequence:

ATGAAAAAGG ACGGCTCCTC AGGTTCCTTC GGAATCAAGG CAAGCCCAGG CTCCCTCTCA 
CGTGCTGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTCCGC 
AGTGATGGAG AGCTGTCCGT TTGTGGGCAC CAGGTGGAAG CCGATGATGA GAACTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTGGACG AGGAGAGTCG ATGGACTGTC 
CACTACACGG CACCATGGCA TCAGCAAGAG AATGTGTTCC TTCCCGCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCAAGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCGGCT CCAGCCCCTG CGACGATTAT CAAGATGAAG ACACAGAAGC AGACCGAAAG 
TGCTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATCGGAA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCCGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC GCGGCAGCAA GCCCAGACGG TCCAGGCTGA CGTGGTTCCT 
ATAAACATAA CTGGGGAGAA TTTCGATCGT CAGGCCAGCC TTCGGCGGTC TCTAATTTAC 
ACAGACACTC TGGTAAGACG ACCGAAGAAA GTCAAAAGGA GAAAGACAAT TTCAGGAATC 
CCTGACATCA TTCAGAAGGA GCTAGCATCA GCCACTGGCC AAGATGATGA TGGTAGTGCC 
CATTCGCTGT ACGTCCCAGA CCACTACTCT ACGTTAGGAA GGCTTGATAG TTATCGGTCC 
ACCGGGCAAT GCTTGGAAAC CAGGGACACC AGCTGTCAGA CCGAAGACGT GAAGGTCATC 
CCACCATCAA TGAGAAGGAT CAGGGCTCAC AAGGGGGTAG GCGTTGCTGC CCAGATGAGC 
CACCTCTCAG GTTCCTCAGG CAACATGTCT GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC 
CCATCTCGCC TCAGTAACGA TACTGGTTTC CACAGTCTTC CACGTACCGG CCCGAGAGCA 
AGCACCTATT CACTGGAGGG AAGGATGGGT GCCCTGGGCT CTACTGAAGA TACGGATGAC 
ACTTCTCCCT ACCAGGGGGG TAGCTTACAG GGGCATGAAA ACTTTGCGCA TTTAGGAGGT 
GCGTCGAGTA CTGGGATGCT TTCGAGGCCC AAATCCCAGC AGTTGAGGTT TTTGGAGAGT 
CCAGCATGTG TGGTTTCACC TCATGCAGCC TACTCTACCA GTGTCATCCC AAATGCCACG 
CTACTGTCCT CTTCAGAGGT TATTGTCATT CACACTGCTC AGAGTGCAGG ACAGCTGGAC 
AGTAGAACAC CCGGTTCATC TTCATATTCA AAAATAAAAC CCAGAGACCG TCCCACACCC 
AGATGTTCTG TGAAAGATGA CCATCAGTCC CCCCGTCATC ACTGGAACGA GGGTCATCTC 
ATTCATTCAC GGGCTTTAGC CTCCTCTGTC CCTGGTGCCA CCACGCTGTT ATCCCTCCAT 
GATTCAGAGG TCTCTCTAAA TGCCCCAGCA AACAGGGAAA ATGGATCCCA AGCCATACTC 
TATCATTGTA GAAACAACCC AAGCTTTCCT GACCACCCTT CAGATGTGGA TGGCAAGAGC 
GAGTGTAGTT ATTCGGGGGA CAGGGGATGT GGCAGTTCTG AGCCCTGGGA ATATAAAACC 
TCCAGCAATG GGCGGGCATC CCCACTGAAG CCACACCTGG CCACTCCTGG TTGCTCTACT 
CCCACAAGTA ACGTGAGCAG CTGCAGTTTG GACCAAACAT CTCTCAAGGG TGATACCAGG 
TCCCTGTGTT CAGAGGACCA TGATGGTTAC TATACTACCA CTCACGAGGC TGGGAACCTG 
TACACTCTCA GTGATGGCTT GGGGAACCCC AGGCACAGCA TGGTCAATGT TTTTGATGGA 
AGGGCTCAGA GAAGCCAAGG GGATCAGGCC GCTCACCAGG ATAAAATCCT TTCGAGAAAC 
ATCTCTCTGA AGAAAGCAAA GAAACCACCC CTGCCACCAT CCCGGACCGA TTCTCTGCGA 
AGGATTCCCA AAAAGAACAA CCAGACAAAC GGTCAGGTGC TCAATGAGAG CCTGATCGCC 
TCGCTCCAGC ATTCACTGCA GCTAAGCCTC CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG 
AGCCCCTGCA GTGACTTTGA GGAACCCTGG CTCCCCAGGT CCAGAAGCCA GAGCATTGTT 
AGCGAGGGTA GTAGCTTGAC CTCCACCACC ACTCCCAACG TGTATTCTCT GTGTGGGGTC 
ACTCCGTCCC AGAGCGACAC AAGTAGCGTC AAGTCGGAAT ACACGGACCC TTGGGGTTAC 
TACATTGACT ACACGAGCTT GCAGGAAGAT CCAGGGAACC CCACCGGGGG CTGCTCAGCC 
AACACTGAGG CAGCAACTGG AAACGGGCCA GTACGCCACA TCCAGGAGGG GTCGAGAGTC 
CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT AGACCAAAGA TCGCCTCACC AGAGAAGTCA 
CAGAGAGTCA CCTCTCCATC CAGTGGGTAT TCTAGCCAGT CGAATACACC CACAGCACTC 
ACCCCTGTGC CTGTGTTTTT AAAATCAATG TCACCAGCAA ATGGAAAGGG GAAGGCCAAG 
CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG ATCTCTTCTA TGTCCATCTC ATCATCGTCC 
ACTTCTCTCT CTTCTAATAC TTCTACCGAA GGAAGTGGGA CCATGAAGAA ACTGGATACA 
ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT 
CTGGCAGACA AGTCCCCTTT TCTTCCACCA CCTCCTCCTC TAGCAGATTG CTCCGAGGGC 
TCTCCTCTGC CGCCCTCTCC CATGTTCCCC CCTCCACCAC CAGAAGCCCT TGTTCCCTTC 
TGCTCCCCCA CCGACGGGTG CCTTTCTCCT TCTCCCACAG CAGTTAGCCC CTCTCTTCCA 
AGATCCTTGC CTCCTGTGCC AGCACCTCCA CCTTTCTTGC CCTCATCAGA ACCCCCGCCC 
GCTCCGCCTC TGGACCCCAA ACTCATGAAA GAAAACAGGC CCTTTTTCAA AAACTCTAGC 
CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA 
CCCCCCATGC CCCTGATCAC CACAGAAGCT TTGCAGATGG TGCAGTTGAG GCCAGTGAGG 
AAGAACTCAG GTGCCGGGGC AGTCCTGTTT TCTGAACCAT CAGCTCAGGA ACAACGAACT 
CCGACTGCTC CACAGTATCA CTTAAAGCCA TCTGCTTTCC TCAAATCCCG AAATAGCATA 
AATGAAATGG AGAGTGAAAG CCAGGCTGCC TCTGTGACGA GCTCGCTTCC GATGCCTGCC 
AAGAGCCAGA GTCAGGGTGA CCATGACAGT GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC 
AGCGATGGAG CTCCAGGCCC GGGTCCCAGC CTCAGGACCA CTCTGCTCCC AGACTCTTCA 
CCCAGCAGGA AGCCACCCCC CATCTCTAAG AAGCCCAAAC TGTTCCTGGT GGTACCACCT 
CCGCAGAGAG ATTTCACAGC GGAGCCCACA GAGAACGGGA GCGAAGCTTT CCCAGGCGTG 
CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA 
GCATCCCGAG CTGGCTCTCA TGCTACGGCC CCCACCCCCG GCAGTCCGGC TCTAGAACCG 
GGAACTGCAG GGTCCTTATC CTCTAGCATT GTGGAAGCCA ATGTCCCCAT GGTCCAACCT 
AATACATCAC CGGGCCCCAC ACAGGAAGAG TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA 
AATGCGAAGA GCTGCCTGTC TCAACAGGGC AGAGAAGCTG GCTTGCTGGA ACCCAACACA 
GCTGCTTCCT CTTCAGACCC CGTGGATGTA TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG 
ACTCCGACTA AACCCAGGAC AACAGAAGAC CTGTTTGCAG CCATTCACAG ATCTAAAAGG 
AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC GATCACACTC GAAATCACTC TCCCTCCCCG 
CCTGTGACAC CCACGAGTGC TGCCCCTAAC CTGGCCTCTC CAAAGCAAGT AGGGTCTATC 
CAGAGAAGCA TAAAAAAGAG CACTACCAGC AGTGACAACT TCAAAGCTCT GCTGCTAAAG 
AAGGGGAGTC GCTCGGACAC CAGCGCTCGC ATGTCAGCGG CTGAGATGCT CAAGTCTACG 
GACCCTCGCT TCCAGAGATC TAGGTCGGAG CCTTCGGCAG ACAGCCCGGA CAGCCCATCC 
AGCTGCTCCC CCAACAAGAA CAGGAGAGCT CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG 
ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT CGGTACAGCC GCAGCAGGAC GCCACCCTCT 
GCGGCCAGCA GCAGGTACAG CATGCGGAAC CGCATCCAGA GCAGCCCCAT GACCGTCATC 
TCCGAGGGAG AGGGAGAACC TGCTGAGCCA GCGGACAACA AAGCACGCAG GGCCCTGGAT 
GCTACCAGGG TGTGCTCTCT GGACAGACTG ACGGGTCAGG AGATGGACCA GGCCAGCCTC 
CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT GGGATAGGAA GGGCTGAGGG CAACGGTCCC 
TCGGAGCAGT GTGGGGGTAC GGAACAGAAG AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006512688.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512751.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu43845
Clone ID Related Accession (Same CDS sequence)	XM_006512688.3 , XM_006512688.4
Accession Version	XM_006512688.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512688.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG 
AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT 
CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA 
GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGCATCAGC CACTGGCCAA 
GATGATGATG GTAGTGCCCA TTCGCTGTAC GTCCCAGACC ACTACTCTAC GTTAGGAAGG 
CTTGATAGTT ATCGGTCCAC CGGGCAATGC TTGGAAACCA GGGACACCAG CTGTCAGACC 
GAAGACGTGA AGGTCATCCC ACCATCAATG AGAAGGATCA GGGCTCACAA GGGGGTAGGC 
GTTGCTGCCC AGATGAGCCA CCTCTCAGGT TCCTCAGGCA ACATGTCTGT GCTGAGTGAC 
TCAGCCGGTG TTGTGTTCCC ATCTCGCCTC AGTAACGATA CTGGTTTCCA CAGTCTTCCA 
CGTACCGGCC CGAGAGCAAG CACCTATTCA CTGGAGGGAA GGATGGGTGC CCTGGGCTCT 
ACTGAAGATA CGGATGACAC TTCTCCCTAC CAGGGGGGTA GCTTACAGGG GCATGAAAAC 
TTTGCGCATT TAGGAGGTGC GTCGAGTACT GGGATGCTTT CGAGGCCCAA ATCCCAGCAG 
TTGAGGTTTT TGGAGAGTCC AGCATGTGTG GTTTCACCTC ATGCAGCCTA CTCTACCAGT 
GTCATCCCAA ATGCCACGCT ACTGTCCTCT TCAGAGGTTA TTGTCATTCA CACTGCTCAG 
AGTGCAGGAC AGCTGGACAG TAGAACACCC GGTTCATCTT CATATTCAAA AATAAAACCC 
AGAGACCGTC CCACACCCAG ATGTTCTGTG AAAGATGACC ATCAGTCCCC CCGTCATCAC 
TGGAACGAGG GTCATCTCAT TCATTCACGG GCTTTAGCCT CCTCTGTCCC TGGTGCCACC 
ACGCTGTTAT CCCTCCATGA TTCAGAGGTC TCTCTAAATG CCCCAGCAAA CAGGGAAAAT 
GGATCCCAAG CCATACTCTA TCATTGTAGA AACAACCCAA GCTTTCCTGA CCACCCTTCA 
GATGTGGATG GCAAGAGCGA GTGTAGTTAT TCGGGGGACA GGGGATGTGG CAGTTCTGAG 
CCCTGGGAAT ATAAAACCTC CAGCAATGGG CGGGCATCCC CACTGAAGCC ACACCTGGCC 
ACTCCTGGTT GCTCTACTCC CACAAGTAAC GTGAGCAGCT GCAGTTTGGA CCAAACATCT 
CTCAAGGGTG ATACCAGGTC CCTGTGTTCA GAGGACCATG ATGGTTACTA TACTACCACT 
CACGAGGCTG GGAACCTGTA CACTCTCAGT GATGGCTTGG GGAACCCCAG GCACAGCATG 
GTCAATGTTT TTGATGGAAG GGCTCAGAGA AGCCAAGGGG ATCAGGCCGC TCACCAGGAT 
AAAATCCTTT CGAGAAACAT CTCTCTGAAG AAAGCAAAGA AACCACCCCT GCCACCATCC 
CGGACCGATT CTCTGCGAAG GATTCCCAAA AAGAACAACC AGACAAACGG TCAGGTGCTC 
AATGAGAGCC TGATCGCCTC GCTCCAGCAT TCACTGCAGC TAAGCCTCCC TGGCAAGGGC 
GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT GACTTTGAGG AACCCTGGCT CCCCAGGTCC 
AGAAGCCAGA GCATTGTTAG CGAGGGTAGT AGCTTGACCT CCACCACCAC TCCCAACGTG 
TATTCTCTGT GTGGGGTCAC TCCGTCCCAG AGCGACACAA GTAGCGTCAA GTCGGAATAC 
ACGGACCCTT GGGGTTACTA CATTGACTAC ACGAGCTTGC AGGAAGATCC AGGGAACCCC 
ACCGGGGGCT GCTCAGCCAA CACTGAGGCA GCAACTGGAA ACGGGCCAGT ACGCCACATC 
CAGGAGGGGT CGAGAGTCCC AGTGCCCCAA GTGCCGGGCT GCTCAGTTAG ACCAAAGATC 
GCCTCACCAG AGAAGTCACA GAGAGTCACC TCTCCATCCA GTGGGTATTC TAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAATGTC ACCAGCAAAT 
GGAAAGGGGA AGGCCAAGCC CAAGGTACCA GAAAGAAAAT CGTCTCTGAT CTCTTCTATG 
TCCATCTCAT CATCGTCCAC TTCTCTCTCT TCTAATACTT CTACCGAAGG AAGTGGGACC 
ATGAAGAAAC TGGATACAAC CCTGGCCTCG GCCCTTGCCC CTCCCCCTCC TCCTCTTCCC 
CCTCTGCCTT CTCCGTGTCT GGCAGACAAG TCCCCTTTTC TTCCACCACC TCCTCCTCTA 
GCAGATTGCT CCGAGGGCTC TCCTCTGCCG CCCTCTCCCA TGTTCCCCCC TCCACCACCA 
GAAGCCCTTG TTCCCTTCTG CTCCCCCACC GACGGGTGCC TTTCTCCTTC TCCCACAGCA 
GTTAGCCCCT CTCTTCCAAG ATCCTTGCCT CCTGTGCCAG CACCTCCACC TTTCTTGCCC 
TCATCAGAAC CCCCGCCCGC TCCGCCTCTG GACCCCAAAC TCATGAAAGA AAACAGGCCC 
TTTTTCAAAA ACTCTAGCCA GTCCGAATCC TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC 
AAGGAGGAGG GCTGCCGACC CCCCATGCCC CTGATCACCA CAGAAGCTTT GCAGATGGTG 
CAGTTGAGGC CAGTGAGGAA GAACTCAGGT GCCGGGGCAG TCCTGTTTTC TGAACCATCA 
GCTCAGGAAC AACGAACTCC GACTGCTCCA CAGTATCACT TAAAGCCATC TGCTTTCCTC 
AAATCCCGAA ATAGCATAAA TGAAATGGAG AGTGAAAGCC AGGCTGCCTC TGTGACGAGC 
TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT CAGGGTGACC ATGACAGTGC AGTAGAGCGT 
GGCGGCCTTC CGAGCTGCAG CGATGGAGCT CCAGGCCCGG GTCCCAGCCT CAGGACCACT 
CTGCTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TCTCTAAGAA GCCCAAACTG 
TTCCTGGTGG TACCACCTCC GCAGAGAGAT TTCACAGCGG AGCCCACAGA GAACGGGAGC 
GAAGCTTTCC CAGGCGTGCC CAGCCCTACC AGGGCAGAGG GGGAAGCTGT GCGGAGCCAA 
GAGGAGAAAT CCAGCCCAGC ATCCCGAGCT GGCTCTCATG CTACGGCCCC CACCCCCGGC 
AGTCCGGCTC TAGAACCGGG AACTGCAGGG TCCTTATCCT CTAGCATTGT GGAAGCCAAT 
GTCCCCATGG TCCAACCTAA TACATCACCG GGCCCCACAC AGGAAGAGTC AGGCGAGAAC 
AGTGTGGATG GTGAGAGAAA TGCGAAGAGC TGCCTGTCTC AACAGGGCAG AGAAGCTGGC 
TTGCTGGAAC CCAACACAGC TGCTTCCTCT TCAGACCCCG TGGATGTATC TAAGGAAGAG 
GGAAGCGATG AGGTGCTGAC TCCGACTAAA CCCAGGACAA CAGAAGACCT GTTTGCAGCC 
ATTCACAGAT CTAAAAGGAA AGTCCTTGGC CGGAAAGACT CAGAAGACGA TCACACTCGA 
AATCACTCTC CCTCCCCGCC TGTGACACCC ACGAGTGCTG CCCCTAACCT GGCCTCTCCA 
AAGCAAGTAG GGTCTATCCA GAGAAGCATA AAAAAGAGCA CTACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTAAAGAA GGGGAGTCGC TCGGACACCA GCGCTCGCAT GTCAGCGGCT 
GAGATGCTCA AGTCTACGGA CCCTCGCTTC CAGAGATCTA GGTCGGAGCC TTCGGCAGAC 
AGCCCGGACA GCCCATCCAG CTGCTCCCCC AACAAGAACA GGAGAGCTCA GGAAGAGTGG 
GCCAAGAACG AAGGCCTGAT GCCTCGGAGT CTGTCCTTTT CGGGCCCTCG GTACAGCCGC 
AGCAGGACGC CACCCTCTGC GGCCAGCAGC AGGTACAGCA TGCGGAACCG CATCCAGAGC 
AGCCCCATGA CCGTCATCTC CGAGGGAGAG GGAGAACCTG CTGAGCCAGC GGACAACAAA 
GCACGCAGGG CCCTGGATGC TACCAGGGTG TGCTCTCTGG ACAGACTGAC GGGTCAGGAG 
ATGGACCAGG CCAGCCTCCT CTGTAGCGAG GAGCCTGCCT CTGTGGATGG GATAGGAAGG 
GCTGAGGGCA ACGGTCCCTC GGAGCAGTGT GGGGGTACGG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512751.1
CDS	476..5329
Translation	MPFHQRSVEPARLRRPDEAALARAGDPPLFRSLEQVSSHTLVCLLAQLADLSRCAGDIFGELEGQAAALGHRTAELHRRLDALHAAAARLDHRRVKIPVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_006512688.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512688.4

ORF Insert Sequence:

ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG 
AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT 
CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA 
GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGCATCAGC CACTGGCCAA 
GATGATGATG GTAGTGCCCA TTCGCTGTAC GTCCCAGACC ACTACTCTAC GTTAGGAAGG 
CTTGATAGTT ATCGGTCCAC CGGGCAATGC TTGGAAACCA GGGACACCAG CTGTCAGACC 
GAAGACGTGA AGGTCATCCC ACCATCAATG AGAAGGATCA GGGCTCACAA GGGGGTAGGC 
GTTGCTGCCC AGATGAGCCA CCTCTCAGGT TCCTCAGGCA ACATGTCTGT GCTGAGTGAC 
TCAGCCGGTG TTGTGTTCCC ATCTCGCCTC AGTAACGATA CTGGTTTCCA CAGTCTTCCA 
CGTACCGGCC CGAGAGCAAG CACCTATTCA CTGGAGGGAA GGATGGGTGC CCTGGGCTCT 
ACTGAAGATA CGGATGACAC TTCTCCCTAC CAGGGGGGTA GCTTACAGGG GCATGAAAAC 
TTTGCGCATT TAGGAGGTGC GTCGAGTACT GGGATGCTTT CGAGGCCCAA ATCCCAGCAG 
TTGAGGTTTT TGGAGAGTCC AGCATGTGTG GTTTCACCTC ATGCAGCCTA CTCTACCAGT 
GTCATCCCAA ATGCCACGCT ACTGTCCTCT TCAGAGGTTA TTGTCATTCA CACTGCTCAG 
AGTGCAGGAC AGCTGGACAG TAGAACACCC GGTTCATCTT CATATTCAAA AATAAAACCC 
AGAGACCGTC CCACACCCAG ATGTTCTGTG AAAGATGACC ATCAGTCCCC CCGTCATCAC 
TGGAACGAGG GTCATCTCAT TCATTCACGG GCTTTAGCCT CCTCTGTCCC TGGTGCCACC 
ACGCTGTTAT CCCTCCATGA TTCAGAGGTC TCTCTAAATG CCCCAGCAAA CAGGGAAAAT 
GGATCCCAAG CCATACTCTA TCATTGTAGA AACAACCCAA GCTTTCCTGA CCACCCTTCA 
GATGTGGATG GCAAGAGCGA GTGTAGTTAT TCGGGGGACA GGGGATGTGG CAGTTCTGAG 
CCCTGGGAAT ATAAAACCTC CAGCAATGGG CGGGCATCCC CACTGAAGCC ACACCTGGCC 
ACTCCTGGTT GCTCTACTCC CACAAGTAAC GTGAGCAGCT GCAGTTTGGA CCAAACATCT 
CTCAAGGGTG ATACCAGGTC CCTGTGTTCA GAGGACCATG ATGGTTACTA TACTACCACT 
CACGAGGCTG GGAACCTGTA CACTCTCAGT GATGGCTTGG GGAACCCCAG GCACAGCATG 
GTCAATGTTT TTGATGGAAG GGCTCAGAGA AGCCAAGGGG ATCAGGCCGC TCACCAGGAT 
AAAATCCTTT CGAGAAACAT CTCTCTGAAG AAAGCAAAGA AACCACCCCT GCCACCATCC 
CGGACCGATT CTCTGCGAAG GATTCCCAAA AAGAACAACC AGACAAACGG TCAGGTGCTC 
AATGAGAGCC TGATCGCCTC GCTCCAGCAT TCACTGCAGC TAAGCCTCCC TGGCAAGGGC 
GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT GACTTTGAGG AACCCTGGCT CCCCAGGTCC 
AGAAGCCAGA GCATTGTTAG CGAGGGTAGT AGCTTGACCT CCACCACCAC TCCCAACGTG 
TATTCTCTGT GTGGGGTCAC TCCGTCCCAG AGCGACACAA GTAGCGTCAA GTCGGAATAC 
ACGGACCCTT GGGGTTACTA CATTGACTAC ACGAGCTTGC AGGAAGATCC AGGGAACCCC 
ACCGGGGGCT GCTCAGCCAA CACTGAGGCA GCAACTGGAA ACGGGCCAGT ACGCCACATC 
CAGGAGGGGT CGAGAGTCCC AGTGCCCCAA GTGCCGGGCT GCTCAGTTAG ACCAAAGATC 
GCCTCACCAG AGAAGTCACA GAGAGTCACC TCTCCATCCA GTGGGTATTC TAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAATGTC ACCAGCAAAT 
GGAAAGGGGA AGGCCAAGCC CAAGGTACCA GAAAGAAAAT CGTCTCTGAT CTCTTCTATG 
TCCATCTCAT CATCGTCCAC TTCTCTCTCT TCTAATACTT CTACCGAAGG AAGTGGGACC 
ATGAAGAAAC TGGATACAAC CCTGGCCTCG GCCCTTGCCC CTCCCCCTCC TCCTCTTCCC 
CCTCTGCCTT CTCCGTGTCT GGCAGACAAG TCCCCTTTTC TTCCACCACC TCCTCCTCTA 
GCAGATTGCT CCGAGGGCTC TCCTCTGCCG CCCTCTCCCA TGTTCCCCCC TCCACCACCA 
GAAGCCCTTG TTCCCTTCTG CTCCCCCACC GACGGGTGCC TTTCTCCTTC TCCCACAGCA 
GTTAGCCCCT CTCTTCCAAG ATCCTTGCCT CCTGTGCCAG CACCTCCACC TTTCTTGCCC 
TCATCAGAAC CCCCGCCCGC TCCGCCTCTG GACCCCAAAC TCATGAAAGA AAACAGGCCC 
TTTTTCAAAA ACTCTAGCCA GTCCGAATCC TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC 
AAGGAGGAGG GCTGCCGACC CCCCATGCCC CTGATCACCA CAGAAGCTTT GCAGATGGTG 
CAGTTGAGGC CAGTGAGGAA GAACTCAGGT GCCGGGGCAG TCCTGTTTTC TGAACCATCA 
GCTCAGGAAC AACGAACTCC GACTGCTCCA CAGTATCACT TAAAGCCATC TGCTTTCCTC 
AAATCCCGAA ATAGCATAAA TGAAATGGAG AGTGAAAGCC AGGCTGCCTC TGTGACGAGC 
TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT CAGGGTGACC ATGACAGTGC AGTAGAGCGT 
GGCGGCCTTC CGAGCTGCAG CGATGGAGCT CCAGGCCCGG GTCCCAGCCT CAGGACCACT 
CTGCTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TCTCTAAGAA GCCCAAACTG 
TTCCTGGTGG TACCACCTCC GCAGAGAGAT TTCACAGCGG AGCCCACAGA GAACGGGAGC 
GAAGCTTTCC CAGGCGTGCC CAGCCCTACC AGGGCAGAGG GGGAAGCTGT GCGGAGCCAA 
GAGGAGAAAT CCAGCCCAGC ATCCCGAGCT GGCTCTCATG CTACGGCCCC CACCCCCGGC 
AGTCCGGCTC TAGAACCGGG AACTGCAGGG TCCTTATCCT CTAGCATTGT GGAAGCCAAT 
GTCCCCATGG TCCAACCTAA TACATCACCG GGCCCCACAC AGGAAGAGTC AGGCGAGAAC 
AGTGTGGATG GTGAGAGAAA TGCGAAGAGC TGCCTGTCTC AACAGGGCAG AGAAGCTGGC 
TTGCTGGAAC CCAACACAGC TGCTTCCTCT TCAGACCCCG TGGATGTATC TAAGGAAGAG 
GGAAGCGATG AGGTGCTGAC TCCGACTAAA CCCAGGACAA CAGAAGACCT GTTTGCAGCC 
ATTCACAGAT CTAAAAGGAA AGTCCTTGGC CGGAAAGACT CAGAAGACGA TCACACTCGA 
AATCACTCTC CCTCCCCGCC TGTGACACCC ACGAGTGCTG CCCCTAACCT GGCCTCTCCA 
AAGCAAGTAG GGTCTATCCA GAGAAGCATA AAAAAGAGCA CTACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTAAAGAA GGGGAGTCGC TCGGACACCA GCGCTCGCAT GTCAGCGGCT 
GAGATGCTCA AGTCTACGGA CCCTCGCTTC CAGAGATCTA GGTCGGAGCC TTCGGCAGAC 
AGCCCGGACA GCCCATCCAG CTGCTCCCCC AACAAGAACA GGAGAGCTCA GGAAGAGTGG 
GCCAAGAACG AAGGCCTGAT GCCTCGGAGT CTGTCCTTTT CGGGCCCTCG GTACAGCCGC 
AGCAGGACGC CACCCTCTGC GGCCAGCAGC AGGTACAGCA TGCGGAACCG CATCCAGAGC 
AGCCCCATGA CCGTCATCTC CGAGGGAGAG GGAGAACCTG CTGAGCCAGC GGACAACAAA 
GCACGCAGGG CCCTGGATGC TACCAGGGTG TGCTCTCTGG ACAGACTGAC GGGTCAGGAG 
ATGGACCAGG CCAGCCTCCT CTGTAGCGAG GAGCCTGCCT CTGTGGATGG GATAGGAAGG 
GCTGAGGGCA ACGGTCCCTC GGAGCAGTGT GGGGGTACGG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006512689.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512752.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.

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CloneID	OMu43846
Clone ID Related Accession (Same CDS sequence)	XM_006512689.3 , XM_006512689.4
Accession Version	XM_006512689.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512689.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATG CAATATACCG TCTGGAGCCT GTGGCTGATT CCGCCATCCA GTGGGGTGCC 
CTTGCGGTTT CCAACTTGGA CGAGGAGAGT CGATGGACTG TCCACTACAC GGCACCATGG 
CATCAGCAAG AGAATGTGTT CCTTCCCGCC ACAAGACCCC CCTGTGTTGA GGACCTGCAC 
CGCCAAGCCA AGCTCAACCT CAAATCAGTG CTGAGGGAAT GTGACAAGCT GAGGCAAGAT 
GGCTGCCGAA GTTCTCAGTA CTATTCTCAG GGACCCACCT TTGCAGCCGG CTCCAGCCCC 
TGCGACGATT ATCAAGATGA AGACACAGAA GCAGACCGAA AGTGCTCTCT CTCTTCATCT 
GAAGAAGAAA GATTTATCGG AATCAGGAGA CCTAAAACGC CAACCTCAGG TGACTTCTCC 
GACCTTCATA CTCAGACAAA CTGGACCAAG TCCCTGCCGC TGCCAACACC AGAAGAGAAG 
ACGCGGCAGC AAGCCCAGAC GGTCCAGGCT GACGTGGTTC CTATAAACAT AACTGGGGAG 
AATTTCGATC GTCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACA ATTTCAGGAA TCCCTGACAT CATTCAGAAG 
GAGCTAGCAT CAGCCACTGG CCAAGATGAT GATGGTAGTG CCCATTCGCT GTACGTCCCA 
GACCACTACT CTACGTTAGG AAGGCTTGAT AGTTATCGGT CCACCGGGCA ATGCTTGGAA 
ACCAGGGACA CCAGCTGTCA GACCGAAGAC GTGAAGGTCA TCCCACCATC AATGAGAAGG 
ATCAGGGCTC ACAAGGGGGT AGGCGTTGCT GCCCAGATGA GCCACCTCTC AGGTTCCTCA 
GGCAACATGT CTGTGCTGAG TGACTCAGCC GGTGTTGTGT TCCCATCTCG CCTCAGTAAC 
GATACTGGTT TCCACAGTCT TCCACGTACC GGCCCGAGAG CAAGCACCTA TTCACTGGAG 
GGAAGGATGG GTGCCCTGGG CTCTACTGAA GATACGGATG ACACTTCTCC CTACCAGGGG 
GGTAGCTTAC AGGGGCATGA AAACTTTGCG CATTTAGGAG GTGCGTCGAG TACTGGGATG 
CTTTCGAGGC CCAAATCCCA GCAGTTGAGG TTTTTGGAGA GTCCAGCATG TGTGGTTTCA 
CCTCATGCAG CCTACTCTAC CAGTGTCATC CCAAATGCCA CGCTACTGTC CTCTTCAGAG 
GTTATTGTCA TTCACACTGC TCAGAGTGCA GGACAGCTGG ACAGTAGAAC ACCCGGTTCA 
TCTTCATATT CAAAAATAAA ACCCAGAGAC CGTCCCACAC CCAGATGTTC TGTGAAAGAT 
GACCATCAGT CCCCCCGTCA TCACTGGAAC GAGGGTCATC TCATTCATTC ACGGGCTTTA 
GCCTCCTCTG TCCCTGGTGC CACCACGCTG TTATCCCTCC ATGATTCAGA GGTCTCTCTA 
AATGCCCCAG CAAACAGGGA AAATGGATCC CAAGCCATAC TCTATCATTG TAGAAACAAC 
CCAAGCTTTC CTGACCACCC TTCAGATGTG GATGGCAAGA GCGAGTGTAG TTATTCGGGG 
GACAGGGGAT GTGGCAGTTC TGAGCCCTGG GAATATAAAA CCTCCAGCAA TGGGCGGGCA 
TCCCCACTGA AGCCACACCT GGCCACTCCT GGTTGCTCTA CTCCCACAAG TAACGTGAGC 
AGCTGCAGTT TGGACCAAAC ATCTCTCAAG GGTGATACCA GGTCCCTGTG TTCAGAGGAC 
CATGATGGTT ACTATACTAC CACTCACGAG GCTGGGAACC TGTACACTCT CAGTGATGGC 
TTGGGGAACC CCAGGCACAG CATGGTCAAT GTTTTTGATG GAAGGGCTCA GAGAAGCCAA 
GGGGATCAGG CCGCTCACCA GGATAAAATC CTTTCGAGAA ACATCTCTCT GAAGAAAGCA 
AAGAAACCAC CCCTGCCACC ATCCCGGACC GATTCTCTGC GAAGGATTCC CAAAAAGAAC 
AACCAGACAA ACGGTCAGGT GCTCAATGAG AGCCTGATCG CCTCGCTCCA GCATTCACTG 
CAGCTAAGCC TCCCTGGCAA GGGCGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTTT 
GAGGAACCCT GGCTCCCCAG GTCCAGAAGC CAGAGCATTG TTAGCGAGGG TAGTAGCTTG 
ACCTCCACCA CCACTCCCAA CGTGTATTCT CTGTGTGGGG TCACTCCGTC CCAGAGCGAC 
ACAAGTAGCG TCAAGTCGGA ATACACGGAC CCTTGGGGTT ACTACATTGA CTACACGAGC 
TTGCAGGAAG ATCCAGGGAA CCCCACCGGG GGCTGCTCAG CCAACACTGA GGCAGCAACT 
GGAAACGGGC CAGTACGCCA CATCCAGGAG GGGTCGAGAG TCCCAGTGCC CCAAGTGCCG 
GGCTGCTCAG TTAGACCAAA GATCGCCTCA CCAGAGAAGT CACAGAGAGT CACCTCTCCA 
TCCAGTGGGT ATTCTAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT 
TTAAAATCAA TGTCACCAGC AAATGGAAAG GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA 
AAATCGTCTC TGATCTCTTC TATGTCCATC TCATCATCGT CCACTTCTCT CTCTTCTAAT 
ACTTCTACCG AAGGAAGTGG GACCATGAAG AAACTGGATA CAACCCTGGC CTCGGCCCTT 
GCCCCTCCCC CTCCTCCTCT TCCCCCTCTG CCTTCTCCGT GTCTGGCAGA CAAGTCCCCT 
TTTCTTCCAC CACCTCCTCC TCTAGCAGAT TGCTCCGAGG GCTCTCCTCT GCCGCCCTCT 
CCCATGTTCC CCCCTCCACC ACCAGAAGCC CTTGTTCCCT TCTGCTCCCC CACCGACGGG 
TGCCTTTCTC CTTCTCCCAC AGCAGTTAGC CCCTCTCTTC CAAGATCCTT GCCTCCTGTG 
CCAGCACCTC CACCTTTCTT GCCCTCATCA GAACCCCCGC CCGCTCCGCC TCTGGACCCC 
AAACTCATGA AAGAAAACAG GCCCTTTTTC AAAAACTCTA GCCAGTCCGA ATCCTCTAGG 
GAGGCCCTCA GGCGGCCTGC CAACAAGGAG GAGGGCTGCC GACCCCCCAT GCCCCTGATC 
ACCACAGAAG CTTTGCAGAT GGTGCAGTTG AGGCCAGTGA GGAAGAACTC AGGTGCCGGG 
GCAGTCCTGT TTTCTGAACC ATCAGCTCAG GAACAACGAA CTCCGACTGC TCCACAGTAT 
CACTTAAAGC CATCTGCTTT CCTCAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA 
AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT CCGATGCCTG CCAAGAGCCA GAGTCAGGGT 
GACCATGACA GTGCAGTAGA GCGTGGCGGC CTTCCGAGCT GCAGCGATGG AGCTCCAGGC 
CCGGGTCCCA GCCTCAGGAC CACTCTGCTC CCAGACTCTT CACCCAGCAG GAAGCCACCC 
CCCATCTCTA AGAAGCCCAA ACTGTTCCTG GTGGTACCAC CTCCGCAGAG AGATTTCACA 
GCGGAGCCCA CAGAGAACGG GAGCGAAGCT TTCCCAGGCG TGCCCAGCCC TACCAGGGCA 
GAGGGGGAAG CTGTGCGGAG CCAAGAGGAG AAATCCAGCC CAGCATCCCG AGCTGGCTCT 
CATGCTACGG CCCCCACCCC CGGCAGTCCG GCTCTAGAAC CGGGAACTGC AGGGTCCTTA 
TCCTCTAGCA TTGTGGAAGC CAATGTCCCC ATGGTCCAAC CTAATACATC ACCGGGCCCC 
ACACAGGAAG AGTCAGGCGA GAACAGTGTG GATGGTGAGA GAAATGCGAA GAGCTGCCTG 
TCTCAACAGG GCAGAGAAGC TGGCTTGCTG GAACCCAACA CAGCTGCTTC CTCTTCAGAC 
CCCGTGGATG TATCTAAGGA AGAGGGAAGC GATGAGGTGC TGACTCCGAC TAAACCCAGG 
ACAACAGAAG ACCTGTTTGC AGCCATTCAC AGATCTAAAA GGAAAGTCCT TGGCCGGAAA 
GACTCAGAAG ACGATCACAC TCGAAATCAC TCTCCCTCCC CGCCTGTGAC ACCCACGAGT 
GCTGCCCCTA ACCTGGCCTC TCCAAAGCAA GTAGGGTCTA TCCAGAGAAG CATAAAAAAG 
AGCACTACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AGAAGGGGAG TCGCTCGGAC 
ACCAGCGCTC GCATGTCAGC GGCTGAGATG CTCAAGTCTA CGGACCCTCG CTTCCAGAGA 
TCTAGGTCGG AGCCTTCGGC AGACAGCCCG GACAGCCCAT CCAGCTGCTC CCCCAACAAG 
AACAGGAGAG CTCAGGAAGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC 
TTTTCGGGCC CTCGGTACAG CCGCAGCAGG ACGCCACCCT CTGCGGCCAG CAGCAGGTAC 
AGCATGCGGA ACCGCATCCA GAGCAGCCCC ATGACCGTCA TCTCCGAGGG AGAGGGAGAA 
CCTGCTGAGC CAGCGGACAA CAAAGCACGC AGGGCCCTGG ATGCTACCAG GGTGTGCTCT 
CTGGACAGAC TGACGGGTCA GGAGATGGAC CAGGCCAGCC TCCTCTGTAG CGAGGAGCCT 
GCCTCTGTGG ATGGGATAGG AAGGGCTGAG GGCAACGGTC CCTCGGAGCA GTGTGGGGGT 
ACGGAACAGA AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512752.1
CDS	324..5081
Translation	MMNAIYRLEPVADSAIQWGALAVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_006512689.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512689.4

ORF Insert Sequence:

ATGATGAATG CAATATACCG TCTGGAGCCT GTGGCTGATT CCGCCATCCA GTGGGGTGCC 
CTTGCGGTTT CCAACTTGGA CGAGGAGAGT CGATGGACTG TCCACTACAC GGCACCATGG 
CATCAGCAAG AGAATGTGTT CCTTCCCGCC ACAAGACCCC CCTGTGTTGA GGACCTGCAC 
CGCCAAGCCA AGCTCAACCT CAAATCAGTG CTGAGGGAAT GTGACAAGCT GAGGCAAGAT 
GGCTGCCGAA GTTCTCAGTA CTATTCTCAG GGACCCACCT TTGCAGCCGG CTCCAGCCCC 
TGCGACGATT ATCAAGATGA AGACACAGAA GCAGACCGAA AGTGCTCTCT CTCTTCATCT 
GAAGAAGAAA GATTTATCGG AATCAGGAGA CCTAAAACGC CAACCTCAGG TGACTTCTCC 
GACCTTCATA CTCAGACAAA CTGGACCAAG TCCCTGCCGC TGCCAACACC AGAAGAGAAG 
ACGCGGCAGC AAGCCCAGAC GGTCCAGGCT GACGTGGTTC CTATAAACAT AACTGGGGAG 
AATTTCGATC GTCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACA ATTTCAGGAA TCCCTGACAT CATTCAGAAG 
GAGCTAGCAT CAGCCACTGG CCAAGATGAT GATGGTAGTG CCCATTCGCT GTACGTCCCA 
GACCACTACT CTACGTTAGG AAGGCTTGAT AGTTATCGGT CCACCGGGCA ATGCTTGGAA 
ACCAGGGACA CCAGCTGTCA GACCGAAGAC GTGAAGGTCA TCCCACCATC AATGAGAAGG 
ATCAGGGCTC ACAAGGGGGT AGGCGTTGCT GCCCAGATGA GCCACCTCTC AGGTTCCTCA 
GGCAACATGT CTGTGCTGAG TGACTCAGCC GGTGTTGTGT TCCCATCTCG CCTCAGTAAC 
GATACTGGTT TCCACAGTCT TCCACGTACC GGCCCGAGAG CAAGCACCTA TTCACTGGAG 
GGAAGGATGG GTGCCCTGGG CTCTACTGAA GATACGGATG ACACTTCTCC CTACCAGGGG 
GGTAGCTTAC AGGGGCATGA AAACTTTGCG CATTTAGGAG GTGCGTCGAG TACTGGGATG 
CTTTCGAGGC CCAAATCCCA GCAGTTGAGG TTTTTGGAGA GTCCAGCATG TGTGGTTTCA 
CCTCATGCAG CCTACTCTAC CAGTGTCATC CCAAATGCCA CGCTACTGTC CTCTTCAGAG 
GTTATTGTCA TTCACACTGC TCAGAGTGCA GGACAGCTGG ACAGTAGAAC ACCCGGTTCA 
TCTTCATATT CAAAAATAAA ACCCAGAGAC CGTCCCACAC CCAGATGTTC TGTGAAAGAT 
GACCATCAGT CCCCCCGTCA TCACTGGAAC GAGGGTCATC TCATTCATTC ACGGGCTTTA 
GCCTCCTCTG TCCCTGGTGC CACCACGCTG TTATCCCTCC ATGATTCAGA GGTCTCTCTA 
AATGCCCCAG CAAACAGGGA AAATGGATCC CAAGCCATAC TCTATCATTG TAGAAACAAC 
CCAAGCTTTC CTGACCACCC TTCAGATGTG GATGGCAAGA GCGAGTGTAG TTATTCGGGG 
GACAGGGGAT GTGGCAGTTC TGAGCCCTGG GAATATAAAA CCTCCAGCAA TGGGCGGGCA 
TCCCCACTGA AGCCACACCT GGCCACTCCT GGTTGCTCTA CTCCCACAAG TAACGTGAGC 
AGCTGCAGTT TGGACCAAAC ATCTCTCAAG GGTGATACCA GGTCCCTGTG TTCAGAGGAC 
CATGATGGTT ACTATACTAC CACTCACGAG GCTGGGAACC TGTACACTCT CAGTGATGGC 
TTGGGGAACC CCAGGCACAG CATGGTCAAT GTTTTTGATG GAAGGGCTCA GAGAAGCCAA 
GGGGATCAGG CCGCTCACCA GGATAAAATC CTTTCGAGAA ACATCTCTCT GAAGAAAGCA 
AAGAAACCAC CCCTGCCACC ATCCCGGACC GATTCTCTGC GAAGGATTCC CAAAAAGAAC 
AACCAGACAA ACGGTCAGGT GCTCAATGAG AGCCTGATCG CCTCGCTCCA GCATTCACTG 
CAGCTAAGCC TCCCTGGCAA GGGCGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTTT 
GAGGAACCCT GGCTCCCCAG GTCCAGAAGC CAGAGCATTG TTAGCGAGGG TAGTAGCTTG 
ACCTCCACCA CCACTCCCAA CGTGTATTCT CTGTGTGGGG TCACTCCGTC CCAGAGCGAC 
ACAAGTAGCG TCAAGTCGGA ATACACGGAC CCTTGGGGTT ACTACATTGA CTACACGAGC 
TTGCAGGAAG ATCCAGGGAA CCCCACCGGG GGCTGCTCAG CCAACACTGA GGCAGCAACT 
GGAAACGGGC CAGTACGCCA CATCCAGGAG GGGTCGAGAG TCCCAGTGCC CCAAGTGCCG 
GGCTGCTCAG TTAGACCAAA GATCGCCTCA CCAGAGAAGT CACAGAGAGT CACCTCTCCA 
TCCAGTGGGT ATTCTAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT 
TTAAAATCAA TGTCACCAGC AAATGGAAAG GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA 
AAATCGTCTC TGATCTCTTC TATGTCCATC TCATCATCGT CCACTTCTCT CTCTTCTAAT 
ACTTCTACCG AAGGAAGTGG GACCATGAAG AAACTGGATA CAACCCTGGC CTCGGCCCTT 
GCCCCTCCCC CTCCTCCTCT TCCCCCTCTG CCTTCTCCGT GTCTGGCAGA CAAGTCCCCT 
TTTCTTCCAC CACCTCCTCC TCTAGCAGAT TGCTCCGAGG GCTCTCCTCT GCCGCCCTCT 
CCCATGTTCC CCCCTCCACC ACCAGAAGCC CTTGTTCCCT TCTGCTCCCC CACCGACGGG 
TGCCTTTCTC CTTCTCCCAC AGCAGTTAGC CCCTCTCTTC CAAGATCCTT GCCTCCTGTG 
CCAGCACCTC CACCTTTCTT GCCCTCATCA GAACCCCCGC CCGCTCCGCC TCTGGACCCC 
AAACTCATGA AAGAAAACAG GCCCTTTTTC AAAAACTCTA GCCAGTCCGA ATCCTCTAGG 
GAGGCCCTCA GGCGGCCTGC CAACAAGGAG GAGGGCTGCC GACCCCCCAT GCCCCTGATC 
ACCACAGAAG CTTTGCAGAT GGTGCAGTTG AGGCCAGTGA GGAAGAACTC AGGTGCCGGG 
GCAGTCCTGT TTTCTGAACC ATCAGCTCAG GAACAACGAA CTCCGACTGC TCCACAGTAT 
CACTTAAAGC CATCTGCTTT CCTCAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA 
AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT CCGATGCCTG CCAAGAGCCA GAGTCAGGGT 
GACCATGACA GTGCAGTAGA GCGTGGCGGC CTTCCGAGCT GCAGCGATGG AGCTCCAGGC 
CCGGGTCCCA GCCTCAGGAC CACTCTGCTC CCAGACTCTT CACCCAGCAG GAAGCCACCC 
CCCATCTCTA AGAAGCCCAA ACTGTTCCTG GTGGTACCAC CTCCGCAGAG AGATTTCACA 
GCGGAGCCCA CAGAGAACGG GAGCGAAGCT TTCCCAGGCG TGCCCAGCCC TACCAGGGCA 
GAGGGGGAAG CTGTGCGGAG CCAAGAGGAG AAATCCAGCC CAGCATCCCG AGCTGGCTCT 
CATGCTACGG CCCCCACCCC CGGCAGTCCG GCTCTAGAAC CGGGAACTGC AGGGTCCTTA 
TCCTCTAGCA TTGTGGAAGC CAATGTCCCC ATGGTCCAAC CTAATACATC ACCGGGCCCC 
ACACAGGAAG AGTCAGGCGA GAACAGTGTG GATGGTGAGA GAAATGCGAA GAGCTGCCTG 
TCTCAACAGG GCAGAGAAGC TGGCTTGCTG GAACCCAACA CAGCTGCTTC CTCTTCAGAC 
CCCGTGGATG TATCTAAGGA AGAGGGAAGC GATGAGGTGC TGACTCCGAC TAAACCCAGG 
ACAACAGAAG ACCTGTTTGC AGCCATTCAC AGATCTAAAA GGAAAGTCCT TGGCCGGAAA 
GACTCAGAAG ACGATCACAC TCGAAATCAC TCTCCCTCCC CGCCTGTGAC ACCCACGAGT 
GCTGCCCCTA ACCTGGCCTC TCCAAAGCAA GTAGGGTCTA TCCAGAGAAG CATAAAAAAG 
AGCACTACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AGAAGGGGAG TCGCTCGGAC 
ACCAGCGCTC GCATGTCAGC GGCTGAGATG CTCAAGTCTA CGGACCCTCG CTTCCAGAGA 
TCTAGGTCGG AGCCTTCGGC AGACAGCCCG GACAGCCCAT CCAGCTGCTC CCCCAACAAG 
AACAGGAGAG CTCAGGAAGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC 
TTTTCGGGCC CTCGGTACAG CCGCAGCAGG ACGCCACCCT CTGCGGCCAG CAGCAGGTAC 
AGCATGCGGA ACCGCATCCA GAGCAGCCCC ATGACCGTCA TCTCCGAGGG AGAGGGAGAA 
CCTGCTGAGC CAGCGGACAA CAAAGCACGC AGGGCCCTGG ATGCTACCAG GGTGTGCTCT 
CTGGACAGAC TGACGGGTCA GGAGATGGAC CAGGCCAGCC TCCTCTGTAG CGAGGAGCCT 
GCCTCTGTGG ATGGGATAGG AAGGGCTGAG GGCAACGGTC CCTCGGAGCA GTGTGGGGGT 
ACGGAACAGA AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006512690.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512753.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.

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CloneID	OMu43847
Clone ID Related Accession (Same CDS sequence)	XM_006512690.4 , XM_006512690.3
Accession Version	XM_006512690.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512690.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTGCC TGAAAGCGGT TTCCAACTTG GACGAGGAGA GTCGATGGAC TGTCCACTAC 
ACGGCACCAT GGCATCAGCA AGAGAATGTG TTCCTTCCCG CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG 
CTGAGGCAAG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC 
GGCTCCAGCC CCTGCGACGA TTATCAAGAT GAAGACACAG AAGCAGACCG AAAGTGCTCT 
CTCTCTTCAT CTGAAGAAGA AAGATTTATC GGAATCAGGA GACCTAAAAC GCCAACCTCA 
GGTGACTTCT CCGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA 
CCAGAAGAGA AGACGCGGCA GCAAGCCCAG ACGGTCCAGG CTGACGTGGT TCCTATAAAC 
ATAACTGGGG AGAATTTCGA TCGTCAGGCC AGCCTTCGGC GGTCTCTAAT TTACACAGAC 
ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CAATTTCAGG AATCCCTGAC 
ATCATTCAGA AGGAGCTAGC ATCAGCCACT GGCCAAGATG ATGATGGTAG TGCCCATTCG 
CTGTACGTCC CAGACCACTA CTCTACGTTA GGAAGGCTTG ATAGTTATCG GTCCACCGGG 
CAATGCTTGG AAACCAGGGA CACCAGCTGT CAGACCGAAG ACGTGAAGGT CATCCCACCA 
TCAATGAGAA GGATCAGGGC TCACAAGGGG GTAGGCGTTG CTGCCCAGAT GAGCCACCTC 
TCAGGTTCCT CAGGCAACAT GTCTGTGCTG AGTGACTCAG CCGGTGTTGT GTTCCCATCT 
CGCCTCAGTA ACGATACTGG TTTCCACAGT CTTCCACGTA CCGGCCCGAG AGCAAGCACC 
TATTCACTGG AGGGAAGGAT GGGTGCCCTG GGCTCTACTG AAGATACGGA TGACACTTCT 
CCCTACCAGG GGGGTAGCTT ACAGGGGCAT GAAAACTTTG CGCATTTAGG AGGTGCGTCG 
AGTACTGGGA TGCTTTCGAG GCCCAAATCC CAGCAGTTGA GGTTTTTGGA GAGTCCAGCA 
TGTGTGGTTT CACCTCATGC AGCCTACTCT ACCAGTGTCA TCCCAAATGC CACGCTACTG 
TCCTCTTCAG AGGTTATTGT CATTCACACT GCTCAGAGTG CAGGACAGCT GGACAGTAGA 
ACACCCGGTT CATCTTCATA TTCAAAAATA AAACCCAGAG ACCGTCCCAC ACCCAGATGT 
TCTGTGAAAG ATGACCATCA GTCCCCCCGT CATCACTGGA ACGAGGGTCA TCTCATTCAT 
TCACGGGCTT TAGCCTCCTC TGTCCCTGGT GCCACCACGC TGTTATCCCT CCATGATTCA 
GAGGTCTCTC TAAATGCCCC AGCAAACAGG GAAAATGGAT CCCAAGCCAT ACTCTATCAT 
TGTAGAAACA ACCCAAGCTT TCCTGACCAC CCTTCAGATG TGGATGGCAA GAGCGAGTGT 
AGTTATTCGG GGGACAGGGG ATGTGGCAGT TCTGAGCCCT GGGAATATAA AACCTCCAGC 
AATGGGCGGG CATCCCCACT GAAGCCACAC CTGGCCACTC CTGGTTGCTC TACTCCCACA 
AGTAACGTGA GCAGCTGCAG TTTGGACCAA ACATCTCTCA AGGGTGATAC CAGGTCCCTG 
TGTTCAGAGG ACCATGATGG TTACTATACT ACCACTCACG AGGCTGGGAA CCTGTACACT 
CTCAGTGATG GCTTGGGGAA CCCCAGGCAC AGCATGGTCA ATGTTTTTGA TGGAAGGGCT 
CAGAGAAGCC AAGGGGATCA GGCCGCTCAC CAGGATAAAA TCCTTTCGAG AAACATCTCT 
CTGAAGAAAG CAAAGAAACC ACCCCTGCCA CCATCCCGGA CCGATTCTCT GCGAAGGATT 
CCCAAAAAGA ACAACCAGAC AAACGGTCAG GTGCTCAATG AGAGCCTGAT CGCCTCGCTC 
CAGCATTCAC TGCAGCTAAG CCTCCCTGGC AAGGGCGGCA GCTCCCCTTC CCAGAGCCCC 
TGCAGTGACT TTGAGGAACC CTGGCTCCCC AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG 
GGTAGTAGCT TGACCTCCAC CACCACTCCC AACGTGTATT CTCTGTGTGG GGTCACTCCG 
TCCCAGAGCG ACACAAGTAG CGTCAAGTCG GAATACACGG ACCCTTGGGG TTACTACATT 
GACTACACGA GCTTGCAGGA AGATCCAGGG AACCCCACCG GGGGCTGCTC AGCCAACACT 
GAGGCAGCAA CTGGAAACGG GCCAGTACGC CACATCCAGG AGGGGTCGAG AGTCCCAGTG 
CCCCAAGTGC CGGGCTGCTC AGTTAGACCA AAGATCGCCT CACCAGAGAA GTCACAGAGA 
GTCACCTCTC CATCCAGTGG GTATTCTAGC CAGTCGAATA CACCCACAGC ACTCACCCCT 
GTGCCTGTGT TTTTAAAATC AATGTCACCA GCAAATGGAA AGGGGAAGGC CAAGCCCAAG 
GTACCAGAAA GAAAATCGTC TCTGATCTCT TCTATGTCCA TCTCATCATC GTCCACTTCT 
CTCTCTTCTA ATACTTCTAC CGAAGGAAGT GGGACCATGA AGAAACTGGA TACAACCCTG 
GCCTCGGCCC TTGCCCCTCC CCCTCCTCCT CTTCCCCCTC TGCCTTCTCC GTGTCTGGCA 
GACAAGTCCC CTTTTCTTCC ACCACCTCCT CCTCTAGCAG ATTGCTCCGA GGGCTCTCCT 
CTGCCGCCCT CTCCCATGTT CCCCCCTCCA CCACCAGAAG CCCTTGTTCC CTTCTGCTCC 
CCCACCGACG GGTGCCTTTC TCCTTCTCCC ACAGCAGTTA GCCCCTCTCT TCCAAGATCC 
TTGCCTCCTG TGCCAGCACC TCCACCTTTC TTGCCCTCAT CAGAACCCCC GCCCGCTCCG 
CCTCTGGACC CCAAACTCAT GAAAGAAAAC AGGCCCTTTT TCAAAAACTC TAGCCAGTCC 
GAATCCTCTA GGGAGGCCCT CAGGCGGCCT GCCAACAAGG AGGAGGGCTG CCGACCCCCC 
ATGCCCCTGA TCACCACAGA AGCTTTGCAG ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC 
TCAGGTGCCG GGGCAGTCCT GTTTTCTGAA CCATCAGCTC AGGAACAACG AACTCCGACT 
GCTCCACAGT ATCACTTAAA GCCATCTGCT TTCCTCAAAT CCCGAAATAG CATAAATGAA 
ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG ACGAGCTCGC TTCCGATGCC TGCCAAGAGC 
CAGAGTCAGG GTGACCATGA CAGTGCAGTA GAGCGTGGCG GCCTTCCGAG CTGCAGCGAT 
GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG ACCACTCTGC TCCCAGACTC TTCACCCAGC 
AGGAAGCCAC CCCCCATCTC TAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG 
AGAGATTTCA CAGCGGAGCC CACAGAGAAC GGGAGCGAAG CTTTCCCAGG CGTGCCCAGC 
CCTACCAGGG CAGAGGGGGA AGCTGTGCGG AGCCAAGAGG AGAAATCCAG CCCAGCATCC 
CGAGCTGGCT CTCATGCTAC GGCCCCCACC CCCGGCAGTC CGGCTCTAGA ACCGGGAACT 
GCAGGGTCCT TATCCTCTAG CATTGTGGAA GCCAATGTCC CCATGGTCCA ACCTAATACA 
TCACCGGGCC CCACACAGGA AGAGTCAGGC GAGAACAGTG TGGATGGTGA GAGAAATGCG 
AAGAGCTGCC TGTCTCAACA GGGCAGAGAA GCTGGCTTGC TGGAACCCAA CACAGCTGCT 
TCCTCTTCAG ACCCCGTGGA TGTATCTAAG GAAGAGGGAA GCGATGAGGT GCTGACTCCG 
ACTAAACCCA GGACAACAGA AGACCTGTTT GCAGCCATTC ACAGATCTAA AAGGAAAGTC 
CTTGGCCGGA AAGACTCAGA AGACGATCAC ACTCGAAATC ACTCTCCCTC CCCGCCTGTG 
ACACCCACGA GTGCTGCCCC TAACCTGGCC TCTCCAAAGC AAGTAGGGTC TATCCAGAGA 
AGCATAAAAA AGAGCACTAC CAGCAGTGAC AACTTCAAAG CTCTGCTGCT AAAGAAGGGG 
AGTCGCTCGG ACACCAGCGC TCGCATGTCA GCGGCTGAGA TGCTCAAGTC TACGGACCCT 
CGCTTCCAGA GATCTAGGTC GGAGCCTTCG GCAGACAGCC CGGACAGCCC ATCCAGCTGC 
TCCCCCAACA AGAACAGGAG AGCTCAGGAA GAGTGGGCCA AGAACGAAGG CCTGATGCCT 
CGGAGTCTGT CCTTTTCGGG CCCTCGGTAC AGCCGCAGCA GGACGCCACC CTCTGCGGCC 
AGCAGCAGGT ACAGCATGCG GAACCGCATC CAGAGCAGCC CCATGACCGT CATCTCCGAG 
GGAGAGGGAG AACCTGCTGA GCCAGCGGAC AACAAAGCAC GCAGGGCCCT GGATGCTACC 
AGGGTGTGCT CTCTGGACAG ACTGACGGGT CAGGAGATGG ACCAGGCCAG CCTCCTCTGT 
AGCGAGGAGC CTGCCTCTGT GGATGGGATA GGAAGGGCTG AGGGCAACGG TCCCTCGGAG 
CAGTGTGGGG GTACGGAACA GAAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512753.1
CDS	5896..10605
Translation	MFCLKAVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_006512690.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512690.4

ORF Insert Sequence:

ATGTTTTGCC TGAAAGCGGT TTCCAACTTG GACGAGGAGA GTCGATGGAC TGTCCACTAC 
ACGGCACCAT GGCATCAGCA AGAGAATGTG TTCCTTCCCG CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG 
CTGAGGCAAG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC 
GGCTCCAGCC CCTGCGACGA TTATCAAGAT GAAGACACAG AAGCAGACCG AAAGTGCTCT 
CTCTCTTCAT CTGAAGAAGA AAGATTTATC GGAATCAGGA GACCTAAAAC GCCAACCTCA 
GGTGACTTCT CCGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA 
CCAGAAGAGA AGACGCGGCA GCAAGCCCAG ACGGTCCAGG CTGACGTGGT TCCTATAAAC 
ATAACTGGGG AGAATTTCGA TCGTCAGGCC AGCCTTCGGC GGTCTCTAAT TTACACAGAC 
ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CAATTTCAGG AATCCCTGAC 
ATCATTCAGA AGGAGCTAGC ATCAGCCACT GGCCAAGATG ATGATGGTAG TGCCCATTCG 
CTGTACGTCC CAGACCACTA CTCTACGTTA GGAAGGCTTG ATAGTTATCG GTCCACCGGG 
CAATGCTTGG AAACCAGGGA CACCAGCTGT CAGACCGAAG ACGTGAAGGT CATCCCACCA 
TCAATGAGAA GGATCAGGGC TCACAAGGGG GTAGGCGTTG CTGCCCAGAT GAGCCACCTC 
TCAGGTTCCT CAGGCAACAT GTCTGTGCTG AGTGACTCAG CCGGTGTTGT GTTCCCATCT 
CGCCTCAGTA ACGATACTGG TTTCCACAGT CTTCCACGTA CCGGCCCGAG AGCAAGCACC 
TATTCACTGG AGGGAAGGAT GGGTGCCCTG GGCTCTACTG AAGATACGGA TGACACTTCT 
CCCTACCAGG GGGGTAGCTT ACAGGGGCAT GAAAACTTTG CGCATTTAGG AGGTGCGTCG 
AGTACTGGGA TGCTTTCGAG GCCCAAATCC CAGCAGTTGA GGTTTTTGGA GAGTCCAGCA 
TGTGTGGTTT CACCTCATGC AGCCTACTCT ACCAGTGTCA TCCCAAATGC CACGCTACTG 
TCCTCTTCAG AGGTTATTGT CATTCACACT GCTCAGAGTG CAGGACAGCT GGACAGTAGA 
ACACCCGGTT CATCTTCATA TTCAAAAATA AAACCCAGAG ACCGTCCCAC ACCCAGATGT 
TCTGTGAAAG ATGACCATCA GTCCCCCCGT CATCACTGGA ACGAGGGTCA TCTCATTCAT 
TCACGGGCTT TAGCCTCCTC TGTCCCTGGT GCCACCACGC TGTTATCCCT CCATGATTCA 
GAGGTCTCTC TAAATGCCCC AGCAAACAGG GAAAATGGAT CCCAAGCCAT ACTCTATCAT 
TGTAGAAACA ACCCAAGCTT TCCTGACCAC CCTTCAGATG TGGATGGCAA GAGCGAGTGT 
AGTTATTCGG GGGACAGGGG ATGTGGCAGT TCTGAGCCCT GGGAATATAA AACCTCCAGC 
AATGGGCGGG CATCCCCACT GAAGCCACAC CTGGCCACTC CTGGTTGCTC TACTCCCACA 
AGTAACGTGA GCAGCTGCAG TTTGGACCAA ACATCTCTCA AGGGTGATAC CAGGTCCCTG 
TGTTCAGAGG ACCATGATGG TTACTATACT ACCACTCACG AGGCTGGGAA CCTGTACACT 
CTCAGTGATG GCTTGGGGAA CCCCAGGCAC AGCATGGTCA ATGTTTTTGA TGGAAGGGCT 
CAGAGAAGCC AAGGGGATCA GGCCGCTCAC CAGGATAAAA TCCTTTCGAG AAACATCTCT 
CTGAAGAAAG CAAAGAAACC ACCCCTGCCA CCATCCCGGA CCGATTCTCT GCGAAGGATT 
CCCAAAAAGA ACAACCAGAC AAACGGTCAG GTGCTCAATG AGAGCCTGAT CGCCTCGCTC 
CAGCATTCAC TGCAGCTAAG CCTCCCTGGC AAGGGCGGCA GCTCCCCTTC CCAGAGCCCC 
TGCAGTGACT TTGAGGAACC CTGGCTCCCC AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG 
GGTAGTAGCT TGACCTCCAC CACCACTCCC AACGTGTATT CTCTGTGTGG GGTCACTCCG 
TCCCAGAGCG ACACAAGTAG CGTCAAGTCG GAATACACGG ACCCTTGGGG TTACTACATT 
GACTACACGA GCTTGCAGGA AGATCCAGGG AACCCCACCG GGGGCTGCTC AGCCAACACT 
GAGGCAGCAA CTGGAAACGG GCCAGTACGC CACATCCAGG AGGGGTCGAG AGTCCCAGTG 
CCCCAAGTGC CGGGCTGCTC AGTTAGACCA AAGATCGCCT CACCAGAGAA GTCACAGAGA 
GTCACCTCTC CATCCAGTGG GTATTCTAGC CAGTCGAATA CACCCACAGC ACTCACCCCT 
GTGCCTGTGT TTTTAAAATC AATGTCACCA GCAAATGGAA AGGGGAAGGC CAAGCCCAAG 
GTACCAGAAA GAAAATCGTC TCTGATCTCT TCTATGTCCA TCTCATCATC GTCCACTTCT 
CTCTCTTCTA ATACTTCTAC CGAAGGAAGT GGGACCATGA AGAAACTGGA TACAACCCTG 
GCCTCGGCCC TTGCCCCTCC CCCTCCTCCT CTTCCCCCTC TGCCTTCTCC GTGTCTGGCA 
GACAAGTCCC CTTTTCTTCC ACCACCTCCT CCTCTAGCAG ATTGCTCCGA GGGCTCTCCT 
CTGCCGCCCT CTCCCATGTT CCCCCCTCCA CCACCAGAAG CCCTTGTTCC CTTCTGCTCC 
CCCACCGACG GGTGCCTTTC TCCTTCTCCC ACAGCAGTTA GCCCCTCTCT TCCAAGATCC 
TTGCCTCCTG TGCCAGCACC TCCACCTTTC TTGCCCTCAT CAGAACCCCC GCCCGCTCCG 
CCTCTGGACC CCAAACTCAT GAAAGAAAAC AGGCCCTTTT TCAAAAACTC TAGCCAGTCC 
GAATCCTCTA GGGAGGCCCT CAGGCGGCCT GCCAACAAGG AGGAGGGCTG CCGACCCCCC 
ATGCCCCTGA TCACCACAGA AGCTTTGCAG ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC 
TCAGGTGCCG GGGCAGTCCT GTTTTCTGAA CCATCAGCTC AGGAACAACG AACTCCGACT 
GCTCCACAGT ATCACTTAAA GCCATCTGCT TTCCTCAAAT CCCGAAATAG CATAAATGAA 
ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG ACGAGCTCGC TTCCGATGCC TGCCAAGAGC 
CAGAGTCAGG GTGACCATGA CAGTGCAGTA GAGCGTGGCG GCCTTCCGAG CTGCAGCGAT 
GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG ACCACTCTGC TCCCAGACTC TTCACCCAGC 
AGGAAGCCAC CCCCCATCTC TAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG 
AGAGATTTCA CAGCGGAGCC CACAGAGAAC GGGAGCGAAG CTTTCCCAGG CGTGCCCAGC 
CCTACCAGGG CAGAGGGGGA AGCTGTGCGG AGCCAAGAGG AGAAATCCAG CCCAGCATCC 
CGAGCTGGCT CTCATGCTAC GGCCCCCACC CCCGGCAGTC CGGCTCTAGA ACCGGGAACT 
GCAGGGTCCT TATCCTCTAG CATTGTGGAA GCCAATGTCC CCATGGTCCA ACCTAATACA 
TCACCGGGCC CCACACAGGA AGAGTCAGGC GAGAACAGTG TGGATGGTGA GAGAAATGCG 
AAGAGCTGCC TGTCTCAACA GGGCAGAGAA GCTGGCTTGC TGGAACCCAA CACAGCTGCT 
TCCTCTTCAG ACCCCGTGGA TGTATCTAAG GAAGAGGGAA GCGATGAGGT GCTGACTCCG 
ACTAAACCCA GGACAACAGA AGACCTGTTT GCAGCCATTC ACAGATCTAA AAGGAAAGTC 
CTTGGCCGGA AAGACTCAGA AGACGATCAC ACTCGAAATC ACTCTCCCTC CCCGCCTGTG 
ACACCCACGA GTGCTGCCCC TAACCTGGCC TCTCCAAAGC AAGTAGGGTC TATCCAGAGA 
AGCATAAAAA AGAGCACTAC CAGCAGTGAC AACTTCAAAG CTCTGCTGCT AAAGAAGGGG 
AGTCGCTCGG ACACCAGCGC TCGCATGTCA GCGGCTGAGA TGCTCAAGTC TACGGACCCT 
CGCTTCCAGA GATCTAGGTC GGAGCCTTCG GCAGACAGCC CGGACAGCCC ATCCAGCTGC 
TCCCCCAACA AGAACAGGAG AGCTCAGGAA GAGTGGGCCA AGAACGAAGG CCTGATGCCT 
CGGAGTCTGT CCTTTTCGGG CCCTCGGTAC AGCCGCAGCA GGACGCCACC CTCTGCGGCC 
AGCAGCAGGT ACAGCATGCG GAACCGCATC CAGAGCAGCC CCATGACCGT CATCTCCGAG 
GGAGAGGGAG AACCTGCTGA GCCAGCGGAC AACAAAGCAC GCAGGGCCCT GGATGCTACC 
AGGGTGTGCT CTCTGGACAG ACTGACGGGT CAGGAGATGG ACCAGGCCAG CCTCCTCTGT 
AGCGAGGAGC CTGCCTCTGT GGATGGGATA GGAAGGGCTG AGGGCAACGG TCCCTCGGAG 
CAGTGTGGGG GTACGGAACA GAAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_030245003.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100863.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X6, mRNA.

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CloneID	OMu85408
Clone ID Related Accession (Same CDS sequence)	XM_030245003.1
Accession Version	XM_030245003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATG CAATATACCG TCTGGAGCCT GTGGCTGATT CCGCCATCCA GTGGGGTGCC 
CTTGCGGTTT CCAACTTGGA CGAGGAGAGT CGATGGACTG TCCACTACAC GGCACCATGG 
CATCAGCAAG AGAATGTGTT CCTTCCCGCC ACAAGACCCC CCTGTGTTGA GGACCTGCAC 
CGCCAAGCCA AGCTCAACCT CAAATCAGTG CTGAGGGAAT GTGACAAGCT GAGGCAAGAT 
GGCTGCCGAA GTTCTCAGTA CTATTCTCAG GGACCCACCT TTGCAGCCGG CTCCAGCCCC 
TGCGACGATT ATCAAGATGA AGACACAGAA GCAGACCGAA AGTGCTCTCT CTCTTCATCT 
GAAGAAGAAA GATTTATCGG AATCAGGAGA CCTAAAACGC CAACCTCAGG TGACTTCTCC 
GACCTTCATA CTCAGACAAA CTGGACCAAG TCCCTGCCGC TGCCAACACC AGAAGAGAAG 
ACGCGGCAGC AAGCCCAGAC GGTCCAGGCT GACGTGGTTC CTATAAACAT AACTGCATCA 
GCCACTGGCC AAGATGATGA TGGTAGTGCC CATTCGCTGT ACGTCCCAGA CCACTACTCT 
ACGTTAGGAA GGCTTGATAG TTATCGGTCC ACCGGGCAAT GCTTGGAAAC CAGGGACACC 
AGCTGTCAGA CCGAAGACGT GAAGGTCATC CCACCATCAA TGAGAAGGAT CAGGGCTCAC 
AAGGGGGTAG GCGTTGCTGC CCAGATGAGC CACCTCTCAG GTTCCTCAGG CAACATGTCT 
GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC CCATCTCGCC TCAGTAACGA TACTGGTTTC 
CACAGTCTTC CACGTACCGG CCCGAGAGCA AGCACCTATT CACTGGAGGG AAGGATGGGT 
GCCCTGGGCT CTACTGAAGA TACGGATGAC ACTTCTCCCT ACCAGGGGGG TAGCTTACAG 
GGGCATGAAA ACTTTGCGCA TTTAGGAGGT GCGTCGAGTA CTGGGATGCT TTCGAGGCCC 
AAATCCCAGC AGTTGAGGTT TTTGGAGAGT CCAGCATGTG TGGTTTCACC TCATGCAGCC 
TACTCTACCA GTGTCATCCC AAATGCCACG CTACTGTCCT CTTCAGAGGT TATTGTCATT 
CACACTGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAACAC CCGGTTCATC TTCATATTCA 
AAAATAAAAC CCAGAGACCG TCCCACACCC AGATGTTCTG TGAAAGATGA CCATCAGTCC 
CCCCGTCATC ACTGGAACGA GGGTCATCTC ATTCATTCAC GGGCTTTAGC CTCCTCTGTC 
CCTGGTGCCA CCACGCTGTT ATCCCTCCAT GATTCAGAGG TCTCTCTAAA TGCCCCAGCA 
AACAGGGAAA ATGGATCCCA AGCCATACTC TATCATTGTA GAAACAACCC AAGCTTTCCT 
GACCACCCTT CAGATGTGGA TGGCAAGAGC GAGTGTAGTT ATTCGGGGGA CAGGGGATGT 
GGCAGTTCTG AGCCCTGGGA ATATAAAACC TCCAGCAATG GGCGGGCATC CCCACTGAAG 
CCACACCTGG CCACTCCTGG TTGCTCTACT CCCACAAGTA ACGTGAGCAG CTGCAGTTTG 
GACCAAACAT CTCTCAAGGG TGATACCAGG TCCCTGTGTT CAGAGGACCA TGATGGTTAC 
TATACTACCA CTCACGAGGC TGGGAACCTG TACACTCTCA GTGATGGCTT GGGGAACCCC 
AGGCACAGCA TGGTCAATGT TTTTGATGGA AGGGCTCAGA GAAGCCAAGG GGATCAGGCC 
GCTCACCAGG ATAAAATCCT TTCGAGAAAC ATCTCTCTGA AGAAAGCAAA GAAACCACCC 
CTGCCACCAT CCCGGACCGA TTCTCTGCGA AGGATTCCCA AAAAGAACAA CCAGACAAAC 
GGTCAGGTGC TCAATGAGAG CCTGATCGCC TCGCTCCAGC ATTCACTGCA GCTAAGCCTC 
CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG AGCCCCTGCA GTGACTTTGA GGAACCCTGG 
CTCCCCAGGT CCAGAAGCCA GAGCATTGTT AGCGAGGGTA GTAGCTTGAC CTCCACCACC 
ACTCCCAACG TGTATTCTCT GTGTGGGGTC ACTCCGTCCC AGAGCGACAC AAGTAGCGTC 
AAGTCGGAAT ACACGGACCC TTGGGGTTAC TACATTGACT ACACGAGCTT GCAGGAAGAT 
CCAGGGAACC CCACCGGGGG CTGCTCAGCC AACACTGAGG CAGCAACTGG AAACGGGCCA 
GTACGCCACA TCCAGGAGGG GTCGAGAGTC CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT 
AGACCAAAGA TCGCCTCACC AGAGAAGTCA CAGAGAGTCA CCTCTCCATC CAGTGGGTAT 
TCTAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAATG 
TCACCAGCAA ATGGAAAGGG GAAGGCCAAG CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG 
ATCTCTTCTA TGTCCATCTC ATCATCGTCC ACTTCTCTCT CTTCTAATAC TTCTACCGAA 
GGAAGTGGGA CCATGAAGAA ACTGGATACA ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT 
CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT CTGGCAGACA AGTCCCCTTT TCTTCCACCA 
CCTCCTCCTC TAGCAGATTG CTCCGAGGGC TCTCCTCTGC CGCCCTCTCC CATGTTCCCC 
CCTCCACCAC CAGAAGCCCT TGTTCCCTTC TGCTCCCCCA CCGACGGGTG CCTTTCTCCT 
TCTCCCACAG CAGTTAGCCC CTCTCTTCCA AGATCCTTGC CTCCTGTGCC AGCACCTCCA 
CCTTTCTTGC CCTCATCAGA ACCCCCGCCC GCTCCGCCTC TGGACCCCAA ACTCATGAAA 
GAAAACAGGC CCTTTTTCAA AAACTCTAGC CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG 
CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA CCCCCCATGC CCCTGATCAC CACAGAAGCT 
TTGCAGATGG TGCAGTTGAG GCCAGTGAGG AAGAACTCAG GTGCCGGGGC AGTCCTGTTT 
TCTGAACCAT CAGCTCAGGA ACAACGAACT CCGACTGCTC CACAGTATCA CTTAAAGCCA 
TCTGCTTTCC TCAAATCCCG AAATAGCATA AATGAAATGG AGAGTGAAAG CCAGGCTGCC 
TCTGTGACGA GCTCGCTTCC GATGCCTGCC AAGAGCCAGA GTCAGGGTGA CCATGACAGT 
GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC AGCGATGGAG CTCCAGGCCC GGGTCCCAGC 
CTCAGGACCA CTCTGCTCCC AGACTCTTCA CCCAGCAGGA AGCCACCCCC CATCTCTAAG 
AAGCCCAAAC TGTTCCTGGT GGTACCACCT CCGCAGAGAG ATTTCACAGC GGAGCCCACA 
GAGAACGGGA GCGAAGCTTT CCCAGGCGTG CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT 
GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA GCATCCCGAG CTGGCTCTCA TGCTACGGCC 
CCCACCCCCG GCAGTCCGGC TCTAGAACCG GGAACTGCAG GGTCCTTATC CTCTAGCATT 
GTGGAAGCCA ATGTCCCCAT GGTCCAACCT AATACATCAC CGGGCCCCAC ACAGGAAGAG 
TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA AATGCGAAGA GCTGCCTGTC TCAACAGGGC 
AGAGAAGCTG GCTTGCTGGA ACCCAACACA GCTGCTTCCT CTTCAGACCC CGTGGATGTA 
TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG ACTCCGACTA AACCCAGGAC AACAGAAGAC 
CTGTTTGCAG CCATTCACAG ATCTAAAAGG AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC 
GATCACACTC GAAATCACTC TCCCTCCCCG CCTGTGACAC CCACGAGTGC TGCCCCTAAC 
CTGGCCTCTC CAAAGCAAGT AGGGTCTATC CAGAGAAGCA TAAAAAAGAG CACTACCAGC 
AGTGACAACT TCAAAGCTCT GCTGCTAAAG AAGGGGAGTC GCTCGGACAC CAGCGCTCGC 
ATGTCAGCGG CTGAGATGCT CAAGTCTACG GACCCTCGCT TCCAGAGATC TAGGTCGGAG 
CCTTCGGCAG ACAGCCCGGA CAGCCCATCC AGCTGCTCCC CCAACAAGAA CAGGAGAGCT 
CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT 
CGGTACAGCC GCAGCAGGAC GCCACCCTCT GCGGCCAGCA GCAGGTACAG CATGCGGAAC 
CGCATCCAGA GCAGCCCCAT GACCGTCATC TCCGAGGGAG AGGGAGAACC TGCTGAGCCA 
GCGGACAACA AAGCACGCAG GGCCCTGGAT GCTACCAGGG TGTGCTCTCT GGACAGACTG 
ACGGGTCAGG AGATGGACCA GGCCAGCCTC CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT 
GGGATAGGAA GGGCTGAGGG CAACGGTCCC TCGGAGCAGT GTGGGGGTAC GGAACAGAAG 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100863.1
CDS	321..4946
Translation	MMNAIYRLEPVADSAIQWGALAVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_030245003.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245003.1

ORF Insert Sequence:

ATGATGAATG CAATATACCG TCTGGAGCCT GTGGCTGATT CCGCCATCCA GTGGGGTGCC 
CTTGCGGTTT CCAACTTGGA CGAGGAGAGT CGATGGACTG TCCACTACAC GGCACCATGG 
CATCAGCAAG AGAATGTGTT CCTTCCCGCC ACAAGACCCC CCTGTGTTGA GGACCTGCAC 
CGCCAAGCCA AGCTCAACCT CAAATCAGTG CTGAGGGAAT GTGACAAGCT GAGGCAAGAT 
GGCTGCCGAA GTTCTCAGTA CTATTCTCAG GGACCCACCT TTGCAGCCGG CTCCAGCCCC 
TGCGACGATT ATCAAGATGA AGACACAGAA GCAGACCGAA AGTGCTCTCT CTCTTCATCT 
GAAGAAGAAA GATTTATCGG AATCAGGAGA CCTAAAACGC CAACCTCAGG TGACTTCTCC 
GACCTTCATA CTCAGACAAA CTGGACCAAG TCCCTGCCGC TGCCAACACC AGAAGAGAAG 
ACGCGGCAGC AAGCCCAGAC GGTCCAGGCT GACGTGGTTC CTATAAACAT AACTGCATCA 
GCCACTGGCC AAGATGATGA TGGTAGTGCC CATTCGCTGT ACGTCCCAGA CCACTACTCT 
ACGTTAGGAA GGCTTGATAG TTATCGGTCC ACCGGGCAAT GCTTGGAAAC CAGGGACACC 
AGCTGTCAGA CCGAAGACGT GAAGGTCATC CCACCATCAA TGAGAAGGAT CAGGGCTCAC 
AAGGGGGTAG GCGTTGCTGC CCAGATGAGC CACCTCTCAG GTTCCTCAGG CAACATGTCT 
GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC CCATCTCGCC TCAGTAACGA TACTGGTTTC 
CACAGTCTTC CACGTACCGG CCCGAGAGCA AGCACCTATT CACTGGAGGG AAGGATGGGT 
GCCCTGGGCT CTACTGAAGA TACGGATGAC ACTTCTCCCT ACCAGGGGGG TAGCTTACAG 
GGGCATGAAA ACTTTGCGCA TTTAGGAGGT GCGTCGAGTA CTGGGATGCT TTCGAGGCCC 
AAATCCCAGC AGTTGAGGTT TTTGGAGAGT CCAGCATGTG TGGTTTCACC TCATGCAGCC 
TACTCTACCA GTGTCATCCC AAATGCCACG CTACTGTCCT CTTCAGAGGT TATTGTCATT 
CACACTGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAACAC CCGGTTCATC TTCATATTCA 
AAAATAAAAC CCAGAGACCG TCCCACACCC AGATGTTCTG TGAAAGATGA CCATCAGTCC 
CCCCGTCATC ACTGGAACGA GGGTCATCTC ATTCATTCAC GGGCTTTAGC CTCCTCTGTC 
CCTGGTGCCA CCACGCTGTT ATCCCTCCAT GATTCAGAGG TCTCTCTAAA TGCCCCAGCA 
AACAGGGAAA ATGGATCCCA AGCCATACTC TATCATTGTA GAAACAACCC AAGCTTTCCT 
GACCACCCTT CAGATGTGGA TGGCAAGAGC GAGTGTAGTT ATTCGGGGGA CAGGGGATGT 
GGCAGTTCTG AGCCCTGGGA ATATAAAACC TCCAGCAATG GGCGGGCATC CCCACTGAAG 
CCACACCTGG CCACTCCTGG TTGCTCTACT CCCACAAGTA ACGTGAGCAG CTGCAGTTTG 
GACCAAACAT CTCTCAAGGG TGATACCAGG TCCCTGTGTT CAGAGGACCA TGATGGTTAC 
TATACTACCA CTCACGAGGC TGGGAACCTG TACACTCTCA GTGATGGCTT GGGGAACCCC 
AGGCACAGCA TGGTCAATGT TTTTGATGGA AGGGCTCAGA GAAGCCAAGG GGATCAGGCC 
GCTCACCAGG ATAAAATCCT TTCGAGAAAC ATCTCTCTGA AGAAAGCAAA GAAACCACCC 
CTGCCACCAT CCCGGACCGA TTCTCTGCGA AGGATTCCCA AAAAGAACAA CCAGACAAAC 
GGTCAGGTGC TCAATGAGAG CCTGATCGCC TCGCTCCAGC ATTCACTGCA GCTAAGCCTC 
CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG AGCCCCTGCA GTGACTTTGA GGAACCCTGG 
CTCCCCAGGT CCAGAAGCCA GAGCATTGTT AGCGAGGGTA GTAGCTTGAC CTCCACCACC 
ACTCCCAACG TGTATTCTCT GTGTGGGGTC ACTCCGTCCC AGAGCGACAC AAGTAGCGTC 
AAGTCGGAAT ACACGGACCC TTGGGGTTAC TACATTGACT ACACGAGCTT GCAGGAAGAT 
CCAGGGAACC CCACCGGGGG CTGCTCAGCC AACACTGAGG CAGCAACTGG AAACGGGCCA 
GTACGCCACA TCCAGGAGGG GTCGAGAGTC CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT 
AGACCAAAGA TCGCCTCACC AGAGAAGTCA CAGAGAGTCA CCTCTCCATC CAGTGGGTAT 
TCTAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAATG 
TCACCAGCAA ATGGAAAGGG GAAGGCCAAG CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG 
ATCTCTTCTA TGTCCATCTC ATCATCGTCC ACTTCTCTCT CTTCTAATAC TTCTACCGAA 
GGAAGTGGGA CCATGAAGAA ACTGGATACA ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT 
CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT CTGGCAGACA AGTCCCCTTT TCTTCCACCA 
CCTCCTCCTC TAGCAGATTG CTCCGAGGGC TCTCCTCTGC CGCCCTCTCC CATGTTCCCC 
CCTCCACCAC CAGAAGCCCT TGTTCCCTTC TGCTCCCCCA CCGACGGGTG CCTTTCTCCT 
TCTCCCACAG CAGTTAGCCC CTCTCTTCCA AGATCCTTGC CTCCTGTGCC AGCACCTCCA 
CCTTTCTTGC CCTCATCAGA ACCCCCGCCC GCTCCGCCTC TGGACCCCAA ACTCATGAAA 
GAAAACAGGC CCTTTTTCAA AAACTCTAGC CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG 
CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA CCCCCCATGC CCCTGATCAC CACAGAAGCT 
TTGCAGATGG TGCAGTTGAG GCCAGTGAGG AAGAACTCAG GTGCCGGGGC AGTCCTGTTT 
TCTGAACCAT CAGCTCAGGA ACAACGAACT CCGACTGCTC CACAGTATCA CTTAAAGCCA 
TCTGCTTTCC TCAAATCCCG AAATAGCATA AATGAAATGG AGAGTGAAAG CCAGGCTGCC 
TCTGTGACGA GCTCGCTTCC GATGCCTGCC AAGAGCCAGA GTCAGGGTGA CCATGACAGT 
GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC AGCGATGGAG CTCCAGGCCC GGGTCCCAGC 
CTCAGGACCA CTCTGCTCCC AGACTCTTCA CCCAGCAGGA AGCCACCCCC CATCTCTAAG 
AAGCCCAAAC TGTTCCTGGT GGTACCACCT CCGCAGAGAG ATTTCACAGC GGAGCCCACA 
GAGAACGGGA GCGAAGCTTT CCCAGGCGTG CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT 
GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA GCATCCCGAG CTGGCTCTCA TGCTACGGCC 
CCCACCCCCG GCAGTCCGGC TCTAGAACCG GGAACTGCAG GGTCCTTATC CTCTAGCATT 
GTGGAAGCCA ATGTCCCCAT GGTCCAACCT AATACATCAC CGGGCCCCAC ACAGGAAGAG 
TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA AATGCGAAGA GCTGCCTGTC TCAACAGGGC 
AGAGAAGCTG GCTTGCTGGA ACCCAACACA GCTGCTTCCT CTTCAGACCC CGTGGATGTA 
TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG ACTCCGACTA AACCCAGGAC AACAGAAGAC 
CTGTTTGCAG CCATTCACAG ATCTAAAAGG AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC 
GATCACACTC GAAATCACTC TCCCTCCCCG CCTGTGACAC CCACGAGTGC TGCCCCTAAC 
CTGGCCTCTC CAAAGCAAGT AGGGTCTATC CAGAGAAGCA TAAAAAAGAG CACTACCAGC 
AGTGACAACT TCAAAGCTCT GCTGCTAAAG AAGGGGAGTC GCTCGGACAC CAGCGCTCGC 
ATGTCAGCGG CTGAGATGCT CAAGTCTACG GACCCTCGCT TCCAGAGATC TAGGTCGGAG 
CCTTCGGCAG ACAGCCCGGA CAGCCCATCC AGCTGCTCCC CCAACAAGAA CAGGAGAGCT 
CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT 
CGGTACAGCC GCAGCAGGAC GCCACCCTCT GCGGCCAGCA GCAGGTACAG CATGCGGAAC 
CGCATCCAGA GCAGCCCCAT GACCGTCATC TCCGAGGGAG AGGGAGAACC TGCTGAGCCA 
GCGGACAACA AAGCACGCAG GGCCCTGGAT GCTACCAGGG TGTGCTCTCT GGACAGACTG 
ACGGGTCAGG AGATGGACCA GGCCAGCCTC CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT 
GGGATAGGAA GGGCTGAGGG CAACGGTCCC TCGGAGCAGT GTGGGGGTAC GGAACAGAAG 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_030245004.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100864.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu85409
Clone ID Related Accession (Same CDS sequence)	XM_030245004.1
Accession Version	XM_030245004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTCT TCATTAATGC AAAGATTAAA TCTTTCTTTA AACTTTTTAA AAAGAAAACG 
GTTTCCAACT TGGACGAGGA GAGTCGATGG ACTGTCCACT ACACGGCACC ATGGCATCAG 
CAAGAGAATG TGTTCCTTCC CGCCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCCAAGCTCA ACCTCAAATC AGTGCTGAGG GAATGTGACA AGCTGAGGCA AGATGGCTGC 
CGAAGTTCTC AGTACTATTC TCAGGGACCC ACCTTTGCAG CCGGCTCCAG CCCCTGCGAC 
GATTATCAAG ATGAAGACAC AGAAGCAGAC CGAAAGTGCT CTCTCTCTTC ATCTGAAGAA 
GAAAGATTTA TCGGAATCAG GAGACCTAAA ACGCCAACCT CAGGTGACTT CTCCGACCTT 
CATACTCAGA CAAACTGGAC CAAGTCCCTG CCGCTGCCAA CACCAGAAGA GAAGACGCGG 
CAGCAAGCCC AGACGGTCCA GGCTGACGTG GTTCCTATAA ACATAACTGC ATCAGCCACT 
GGCCAAGATG ATGATGGTAG TGCCCATTCG CTGTACGTCC CAGACCACTA CTCTACGTTA 
GGAAGGCTTG ATAGTTATCG GTCCACCGGG CAATGCTTGG AAACCAGGGA CACCAGCTGT 
CAGACCGAAG ACGTGAAGGT CATCCCACCA TCAATGAGAA GGATCAGGGC TCACAAGGGG 
GTAGGCGTTG CTGCCCAGAT GAGCCACCTC TCAGGTTCCT CAGGCAACAT GTCTGTGCTG 
AGTGACTCAG CCGGTGTTGT GTTCCCATCT CGCCTCAGTA ACGATACTGG TTTCCACAGT 
CTTCCACGTA CCGGCCCGAG AGCAAGCACC TATTCACTGG AGGGAAGGAT GGGTGCCCTG 
GGCTCTACTG AAGATACGGA TGACACTTCT CCCTACCAGG GGGGTAGCTT ACAGGGGCAT 
GAAAACTTTG CGCATTTAGG AGGTGCGTCG AGTACTGGGA TGCTTTCGAG GCCCAAATCC 
CAGCAGTTGA GGTTTTTGGA GAGTCCAGCA TGTGTGGTTT CACCTCATGC AGCCTACTCT 
ACCAGTGTCA TCCCAAATGC CACGCTACTG TCCTCTTCAG AGGTTATTGT CATTCACACT 
GCTCAGAGTG CAGGACAGCT GGACAGTAGA ACACCCGGTT CATCTTCATA TTCAAAAATA 
AAACCCAGAG ACCGTCCCAC ACCCAGATGT TCTGTGAAAG ATGACCATCA GTCCCCCCGT 
CATCACTGGA ACGAGGGTCA TCTCATTCAT TCACGGGCTT TAGCCTCCTC TGTCCCTGGT 
GCCACCACGC TGTTATCCCT CCATGATTCA GAGGTCTCTC TAAATGCCCC AGCAAACAGG 
GAAAATGGAT CCCAAGCCAT ACTCTATCAT TGTAGAAACA ACCCAAGCTT TCCTGACCAC 
CCTTCAGATG TGGATGGCAA GAGCGAGTGT AGTTATTCGG GGGACAGGGG ATGTGGCAGT 
TCTGAGCCCT GGGAATATAA AACCTCCAGC AATGGGCGGG CATCCCCACT GAAGCCACAC 
CTGGCCACTC CTGGTTGCTC TACTCCCACA AGTAACGTGA GCAGCTGCAG TTTGGACCAA 
ACATCTCTCA AGGGTGATAC CAGGTCCCTG TGTTCAGAGG ACCATGATGG TTACTATACT 
ACCACTCACG AGGCTGGGAA CCTGTACACT CTCAGTGATG GCTTGGGGAA CCCCAGGCAC 
AGCATGGTCA ATGTTTTTGA TGGAAGGGCT CAGAGAAGCC AAGGGGATCA GGCCGCTCAC 
CAGGATAAAA TCCTTTCGAG AAACATCTCT CTGAAGAAAG CAAAGAAACC ACCCCTGCCA 
CCATCCCGGA CCGATTCTCT GCGAAGGATT CCCAAAAAGA ACAACCAGAC AAACGGTCAG 
GTGCTCAATG AGAGCCTGAT CGCCTCGCTC CAGCATTCAC TGCAGCTAAG CCTCCCTGGC 
AAGGGCGGCA GCTCCCCTTC CCAGAGCCCC TGCAGTGACT TTGAGGAACC CTGGCTCCCC 
AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG GGTAGTAGCT TGACCTCCAC CACCACTCCC 
AACGTGTATT CTCTGTGTGG GGTCACTCCG TCCCAGAGCG ACACAAGTAG CGTCAAGTCG 
GAATACACGG ACCCTTGGGG TTACTACATT GACTACACGA GCTTGCAGGA AGATCCAGGG 
AACCCCACCG GGGGCTGCTC AGCCAACACT GAGGCAGCAA CTGGAAACGG GCCAGTACGC 
CACATCCAGG AGGGGTCGAG AGTCCCAGTG CCCCAAGTGC CGGGCTGCTC AGTTAGACCA 
AAGATCGCCT CACCAGAGAA GTCACAGAGA GTCACCTCTC CATCCAGTGG GTATTCTAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AATGTCACCA 
GCAAATGGAA AGGGGAAGGC CAAGCCCAAG GTACCAGAAA GAAAATCGTC TCTGATCTCT 
TCTATGTCCA TCTCATCATC GTCCACTTCT CTCTCTTCTA ATACTTCTAC CGAAGGAAGT 
GGGACCATGA AGAAACTGGA TACAACCCTG GCCTCGGCCC TTGCCCCTCC CCCTCCTCCT 
CTTCCCCCTC TGCCTTCTCC GTGTCTGGCA GACAAGTCCC CTTTTCTTCC ACCACCTCCT 
CCTCTAGCAG ATTGCTCCGA GGGCTCTCCT CTGCCGCCCT CTCCCATGTT CCCCCCTCCA 
CCACCAGAAG CCCTTGTTCC CTTCTGCTCC CCCACCGACG GGTGCCTTTC TCCTTCTCCC 
ACAGCAGTTA GCCCCTCTCT TCCAAGATCC TTGCCTCCTG TGCCAGCACC TCCACCTTTC 
TTGCCCTCAT CAGAACCCCC GCCCGCTCCG CCTCTGGACC CCAAACTCAT GAAAGAAAAC 
AGGCCCTTTT TCAAAAACTC TAGCCAGTCC GAATCCTCTA GGGAGGCCCT CAGGCGGCCT 
GCCAACAAGG AGGAGGGCTG CCGACCCCCC ATGCCCCTGA TCACCACAGA AGCTTTGCAG 
ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC TCAGGTGCCG GGGCAGTCCT GTTTTCTGAA 
CCATCAGCTC AGGAACAACG AACTCCGACT GCTCCACAGT ATCACTTAAA GCCATCTGCT 
TTCCTCAAAT CCCGAAATAG CATAAATGAA ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG 
ACGAGCTCGC TTCCGATGCC TGCCAAGAGC CAGAGTCAGG GTGACCATGA CAGTGCAGTA 
GAGCGTGGCG GCCTTCCGAG CTGCAGCGAT GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG 
ACCACTCTGC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATCTC TAAGAAGCCC 
AAACTGTTCC TGGTGGTACC ACCTCCGCAG AGAGATTTCA CAGCGGAGCC CACAGAGAAC 
GGGAGCGAAG CTTTCCCAGG CGTGCCCAGC CCTACCAGGG CAGAGGGGGA AGCTGTGCGG 
AGCCAAGAGG AGAAATCCAG CCCAGCATCC CGAGCTGGCT CTCATGCTAC GGCCCCCACC 
CCCGGCAGTC CGGCTCTAGA ACCGGGAACT GCAGGGTCCT TATCCTCTAG CATTGTGGAA 
GCCAATGTCC CCATGGTCCA ACCTAATACA TCACCGGGCC CCACACAGGA AGAGTCAGGC 
GAGAACAGTG TGGATGGTGA GAGAAATGCG AAGAGCTGCC TGTCTCAACA GGGCAGAGAA 
GCTGGCTTGC TGGAACCCAA CACAGCTGCT TCCTCTTCAG ACCCCGTGGA TGTATCTAAG 
GAAGAGGGAA GCGATGAGGT GCTGACTCCG ACTAAACCCA GGACAACAGA AGACCTGTTT 
GCAGCCATTC ACAGATCTAA AAGGAAAGTC CTTGGCCGGA AAGACTCAGA AGACGATCAC 
ACTCGAAATC ACTCTCCCTC CCCGCCTGTG ACACCCACGA GTGCTGCCCC TAACCTGGCC 
TCTCCAAAGC AAGTAGGGTC TATCCAGAGA AGCATAAAAA AGAGCACTAC CAGCAGTGAC 
AACTTCAAAG CTCTGCTGCT AAAGAAGGGG AGTCGCTCGG ACACCAGCGC TCGCATGTCA 
GCGGCTGAGA TGCTCAAGTC TACGGACCCT CGCTTCCAGA GATCTAGGTC GGAGCCTTCG 
GCAGACAGCC CGGACAGCCC ATCCAGCTGC TCCCCCAACA AGAACAGGAG AGCTCAGGAA 
GAGTGGGCCA AGAACGAAGG CCTGATGCCT CGGAGTCTGT CCTTTTCGGG CCCTCGGTAC 
AGCCGCAGCA GGACGCCACC CTCTGCGGCC AGCAGCAGGT ACAGCATGCG GAACCGCATC 
CAGAGCAGCC CCATGACCGT CATCTCCGAG GGAGAGGGAG AACCTGCTGA GCCAGCGGAC 
AACAAAGCAC GCAGGGCCCT GGATGCTACC AGGGTGTGCT CTCTGGACAG ACTGACGGGT 
CAGGAGATGG ACCAGGCCAG CCTCCTCTGT AGCGAGGAGC CTGCCTCTGT GGATGGGATA 
GGAAGGGCTG AGGGCAACGG TCCCTCGGAG CAGTGTGGGG GTACGGAACA GAAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100864.1
CDS	580..5199
Translation	MVVFINAKIKSFFKLFKKKTVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_030245004.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245004.1

ORF Insert Sequence:

ATGGTGGTCT TCATTAATGC AAAGATTAAA TCTTTCTTTA AACTTTTTAA AAAGAAAACG 
GTTTCCAACT TGGACGAGGA GAGTCGATGG ACTGTCCACT ACACGGCACC ATGGCATCAG 
CAAGAGAATG TGTTCCTTCC CGCCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCCAAGCTCA ACCTCAAATC AGTGCTGAGG GAATGTGACA AGCTGAGGCA AGATGGCTGC 
CGAAGTTCTC AGTACTATTC TCAGGGACCC ACCTTTGCAG CCGGCTCCAG CCCCTGCGAC 
GATTATCAAG ATGAAGACAC AGAAGCAGAC CGAAAGTGCT CTCTCTCTTC ATCTGAAGAA 
GAAAGATTTA TCGGAATCAG GAGACCTAAA ACGCCAACCT CAGGTGACTT CTCCGACCTT 
CATACTCAGA CAAACTGGAC CAAGTCCCTG CCGCTGCCAA CACCAGAAGA GAAGACGCGG 
CAGCAAGCCC AGACGGTCCA GGCTGACGTG GTTCCTATAA ACATAACTGC ATCAGCCACT 
GGCCAAGATG ATGATGGTAG TGCCCATTCG CTGTACGTCC CAGACCACTA CTCTACGTTA 
GGAAGGCTTG ATAGTTATCG GTCCACCGGG CAATGCTTGG AAACCAGGGA CACCAGCTGT 
CAGACCGAAG ACGTGAAGGT CATCCCACCA TCAATGAGAA GGATCAGGGC TCACAAGGGG 
GTAGGCGTTG CTGCCCAGAT GAGCCACCTC TCAGGTTCCT CAGGCAACAT GTCTGTGCTG 
AGTGACTCAG CCGGTGTTGT GTTCCCATCT CGCCTCAGTA ACGATACTGG TTTCCACAGT 
CTTCCACGTA CCGGCCCGAG AGCAAGCACC TATTCACTGG AGGGAAGGAT GGGTGCCCTG 
GGCTCTACTG AAGATACGGA TGACACTTCT CCCTACCAGG GGGGTAGCTT ACAGGGGCAT 
GAAAACTTTG CGCATTTAGG AGGTGCGTCG AGTACTGGGA TGCTTTCGAG GCCCAAATCC 
CAGCAGTTGA GGTTTTTGGA GAGTCCAGCA TGTGTGGTTT CACCTCATGC AGCCTACTCT 
ACCAGTGTCA TCCCAAATGC CACGCTACTG TCCTCTTCAG AGGTTATTGT CATTCACACT 
GCTCAGAGTG CAGGACAGCT GGACAGTAGA ACACCCGGTT CATCTTCATA TTCAAAAATA 
AAACCCAGAG ACCGTCCCAC ACCCAGATGT TCTGTGAAAG ATGACCATCA GTCCCCCCGT 
CATCACTGGA ACGAGGGTCA TCTCATTCAT TCACGGGCTT TAGCCTCCTC TGTCCCTGGT 
GCCACCACGC TGTTATCCCT CCATGATTCA GAGGTCTCTC TAAATGCCCC AGCAAACAGG 
GAAAATGGAT CCCAAGCCAT ACTCTATCAT TGTAGAAACA ACCCAAGCTT TCCTGACCAC 
CCTTCAGATG TGGATGGCAA GAGCGAGTGT AGTTATTCGG GGGACAGGGG ATGTGGCAGT 
TCTGAGCCCT GGGAATATAA AACCTCCAGC AATGGGCGGG CATCCCCACT GAAGCCACAC 
CTGGCCACTC CTGGTTGCTC TACTCCCACA AGTAACGTGA GCAGCTGCAG TTTGGACCAA 
ACATCTCTCA AGGGTGATAC CAGGTCCCTG TGTTCAGAGG ACCATGATGG TTACTATACT 
ACCACTCACG AGGCTGGGAA CCTGTACACT CTCAGTGATG GCTTGGGGAA CCCCAGGCAC 
AGCATGGTCA ATGTTTTTGA TGGAAGGGCT CAGAGAAGCC AAGGGGATCA GGCCGCTCAC 
CAGGATAAAA TCCTTTCGAG AAACATCTCT CTGAAGAAAG CAAAGAAACC ACCCCTGCCA 
CCATCCCGGA CCGATTCTCT GCGAAGGATT CCCAAAAAGA ACAACCAGAC AAACGGTCAG 
GTGCTCAATG AGAGCCTGAT CGCCTCGCTC CAGCATTCAC TGCAGCTAAG CCTCCCTGGC 
AAGGGCGGCA GCTCCCCTTC CCAGAGCCCC TGCAGTGACT TTGAGGAACC CTGGCTCCCC 
AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG GGTAGTAGCT TGACCTCCAC CACCACTCCC 
AACGTGTATT CTCTGTGTGG GGTCACTCCG TCCCAGAGCG ACACAAGTAG CGTCAAGTCG 
GAATACACGG ACCCTTGGGG TTACTACATT GACTACACGA GCTTGCAGGA AGATCCAGGG 
AACCCCACCG GGGGCTGCTC AGCCAACACT GAGGCAGCAA CTGGAAACGG GCCAGTACGC 
CACATCCAGG AGGGGTCGAG AGTCCCAGTG CCCCAAGTGC CGGGCTGCTC AGTTAGACCA 
AAGATCGCCT CACCAGAGAA GTCACAGAGA GTCACCTCTC CATCCAGTGG GTATTCTAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AATGTCACCA 
GCAAATGGAA AGGGGAAGGC CAAGCCCAAG GTACCAGAAA GAAAATCGTC TCTGATCTCT 
TCTATGTCCA TCTCATCATC GTCCACTTCT CTCTCTTCTA ATACTTCTAC CGAAGGAAGT 
GGGACCATGA AGAAACTGGA TACAACCCTG GCCTCGGCCC TTGCCCCTCC CCCTCCTCCT 
CTTCCCCCTC TGCCTTCTCC GTGTCTGGCA GACAAGTCCC CTTTTCTTCC ACCACCTCCT 
CCTCTAGCAG ATTGCTCCGA GGGCTCTCCT CTGCCGCCCT CTCCCATGTT CCCCCCTCCA 
CCACCAGAAG CCCTTGTTCC CTTCTGCTCC CCCACCGACG GGTGCCTTTC TCCTTCTCCC 
ACAGCAGTTA GCCCCTCTCT TCCAAGATCC TTGCCTCCTG TGCCAGCACC TCCACCTTTC 
TTGCCCTCAT CAGAACCCCC GCCCGCTCCG CCTCTGGACC CCAAACTCAT GAAAGAAAAC 
AGGCCCTTTT TCAAAAACTC TAGCCAGTCC GAATCCTCTA GGGAGGCCCT CAGGCGGCCT 
GCCAACAAGG AGGAGGGCTG CCGACCCCCC ATGCCCCTGA TCACCACAGA AGCTTTGCAG 
ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC TCAGGTGCCG GGGCAGTCCT GTTTTCTGAA 
CCATCAGCTC AGGAACAACG AACTCCGACT GCTCCACAGT ATCACTTAAA GCCATCTGCT 
TTCCTCAAAT CCCGAAATAG CATAAATGAA ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG 
ACGAGCTCGC TTCCGATGCC TGCCAAGAGC CAGAGTCAGG GTGACCATGA CAGTGCAGTA 
GAGCGTGGCG GCCTTCCGAG CTGCAGCGAT GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG 
ACCACTCTGC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATCTC TAAGAAGCCC 
AAACTGTTCC TGGTGGTACC ACCTCCGCAG AGAGATTTCA CAGCGGAGCC CACAGAGAAC 
GGGAGCGAAG CTTTCCCAGG CGTGCCCAGC CCTACCAGGG CAGAGGGGGA AGCTGTGCGG 
AGCCAAGAGG AGAAATCCAG CCCAGCATCC CGAGCTGGCT CTCATGCTAC GGCCCCCACC 
CCCGGCAGTC CGGCTCTAGA ACCGGGAACT GCAGGGTCCT TATCCTCTAG CATTGTGGAA 
GCCAATGTCC CCATGGTCCA ACCTAATACA TCACCGGGCC CCACACAGGA AGAGTCAGGC 
GAGAACAGTG TGGATGGTGA GAGAAATGCG AAGAGCTGCC TGTCTCAACA GGGCAGAGAA 
GCTGGCTTGC TGGAACCCAA CACAGCTGCT TCCTCTTCAG ACCCCGTGGA TGTATCTAAG 
GAAGAGGGAA GCGATGAGGT GCTGACTCCG ACTAAACCCA GGACAACAGA AGACCTGTTT 
GCAGCCATTC ACAGATCTAA AAGGAAAGTC CTTGGCCGGA AAGACTCAGA AGACGATCAC 
ACTCGAAATC ACTCTCCCTC CCCGCCTGTG ACACCCACGA GTGCTGCCCC TAACCTGGCC 
TCTCCAAAGC AAGTAGGGTC TATCCAGAGA AGCATAAAAA AGAGCACTAC CAGCAGTGAC 
AACTTCAAAG CTCTGCTGCT AAAGAAGGGG AGTCGCTCGG ACACCAGCGC TCGCATGTCA 
GCGGCTGAGA TGCTCAAGTC TACGGACCCT CGCTTCCAGA GATCTAGGTC GGAGCCTTCG 
GCAGACAGCC CGGACAGCCC ATCCAGCTGC TCCCCCAACA AGAACAGGAG AGCTCAGGAA 
GAGTGGGCCA AGAACGAAGG CCTGATGCCT CGGAGTCTGT CCTTTTCGGG CCCTCGGTAC 
AGCCGCAGCA GGACGCCACC CTCTGCGGCC AGCAGCAGGT ACAGCATGCG GAACCGCATC 
CAGAGCAGCC CCATGACCGT CATCTCCGAG GGAGAGGGAG AACCTGCTGA GCCAGCGGAC 
AACAAAGCAC GCAGGGCCCT GGATGCTACC AGGGTGTGCT CTCTGGACAG ACTGACGGGT 
CAGGAGATGG ACCAGGCCAG CCTCCTCTGT AGCGAGGAGC CTGCCTCTGT GGATGGGATA 
GGAAGGGCTG AGGGCAACGG TCCCTCGGAG CAGTGTGGGG GTACGGAACA GAAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_030245005.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100865.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu85410
Clone ID Related Accession (Same CDS sequence)	XM_030245005.1
Accession Version	XM_030245005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTGCC TGAAAGCGGT TTCCAACTTG GACGAGGAGA GTCGATGGAC TGTCCACTAC 
ACGGCACCAT GGCATCAGCA AGAGAATGTG TTCCTTCCCG CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG 
CTGAGGCAAG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC 
GGCTCCAGCC CCTGCGACGA TTATCAAGAT GAAGACACAG AAGCAGACCG AAAGTGCTCT 
CTCTCTTCAT CTGAAGAAGA AAGATTTATC GGAATCAGGA GACCTAAAAC GCCAACCTCA 
GGTGACTTCT CCGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA 
CCAGAAGAGA AGACGCGGCA GCAAGCCCAG ACGGTCCAGG CTGACGTGGT TCCTATAAAC 
ATAACTGCAT CAGCCACTGG CCAAGATGAT GATGGTAGTG CCCATTCGCT GTACGTCCCA 
GACCACTACT CTACGTTAGG AAGGCTTGAT AGTTATCGGT CCACCGGGCA ATGCTTGGAA 
ACCAGGGACA CCAGCTGTCA GACCGAAGAC GTGAAGGTCA TCCCACCATC AATGAGAAGG 
ATCAGGGCTC ACAAGGGGGT AGGCGTTGCT GCCCAGATGA GCCACCTCTC AGGTTCCTCA 
GGCAACATGT CTGTGCTGAG TGACTCAGCC GGTGTTGTGT TCCCATCTCG CCTCAGTAAC 
GATACTGGTT TCCACAGTCT TCCACGTACC GGCCCGAGAG CAAGCACCTA TTCACTGGAG 
GGAAGGATGG GTGCCCTGGG CTCTACTGAA GATACGGATG ACACTTCTCC CTACCAGGGG 
GGTAGCTTAC AGGGGCATGA AAACTTTGCG CATTTAGGAG GTGCGTCGAG TACTGGGATG 
CTTTCGAGGC CCAAATCCCA GCAGTTGAGG TTTTTGGAGA GTCCAGCATG TGTGGTTTCA 
CCTCATGCAG CCTACTCTAC CAGTGTCATC CCAAATGCCA CGCTACTGTC CTCTTCAGAG 
GTTATTGTCA TTCACACTGC TCAGAGTGCA GGACAGCTGG ACAGTAGAAC ACCCGGTTCA 
TCTTCATATT CAAAAATAAA ACCCAGAGAC CGTCCCACAC CCAGATGTTC TGTGAAAGAT 
GACCATCAGT CCCCCCGTCA TCACTGGAAC GAGGGTCATC TCATTCATTC ACGGGCTTTA 
GCCTCCTCTG TCCCTGGTGC CACCACGCTG TTATCCCTCC ATGATTCAGA GGTCTCTCTA 
AATGCCCCAG CAAACAGGGA AAATGGATCC CAAGCCATAC TCTATCATTG TAGAAACAAC 
CCAAGCTTTC CTGACCACCC TTCAGATGTG GATGGCAAGA GCGAGTGTAG TTATTCGGGG 
GACAGGGGAT GTGGCAGTTC TGAGCCCTGG GAATATAAAA CCTCCAGCAA TGGGCGGGCA 
TCCCCACTGA AGCCACACCT GGCCACTCCT GGTTGCTCTA CTCCCACAAG TAACGTGAGC 
AGCTGCAGTT TGGACCAAAC ATCTCTCAAG GGTGATACCA GGTCCCTGTG TTCAGAGGAC 
CATGATGGTT ACTATACTAC CACTCACGAG GCTGGGAACC TGTACACTCT CAGTGATGGC 
TTGGGGAACC CCAGGCACAG CATGGTCAAT GTTTTTGATG GAAGGGCTCA GAGAAGCCAA 
GGGGATCAGG CCGCTCACCA GGATAAAATC CTTTCGAGAA ACATCTCTCT GAAGAAAGCA 
AAGAAACCAC CCCTGCCACC ATCCCGGACC GATTCTCTGC GAAGGATTCC CAAAAAGAAC 
AACCAGACAA ACGGTCAGGT GCTCAATGAG AGCCTGATCG CCTCGCTCCA GCATTCACTG 
CAGCTAAGCC TCCCTGGCAA GGGCGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTTT 
GAGGAACCCT GGCTCCCCAG GTCCAGAAGC CAGAGCATTG TTAGCGAGGG TAGTAGCTTG 
ACCTCCACCA CCACTCCCAA CGTGTATTCT CTGTGTGGGG TCACTCCGTC CCAGAGCGAC 
ACAAGTAGCG TCAAGTCGGA ATACACGGAC CCTTGGGGTT ACTACATTGA CTACACGAGC 
TTGCAGGAAG ATCCAGGGAA CCCCACCGGG GGCTGCTCAG CCAACACTGA GGCAGCAACT 
GGAAACGGGC CAGTACGCCA CATCCAGGAG GGGTCGAGAG TCCCAGTGCC CCAAGTGCCG 
GGCTGCTCAG TTAGACCAAA GATCGCCTCA CCAGAGAAGT CACAGAGAGT CACCTCTCCA 
TCCAGTGGGT ATTCTAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT 
TTAAAATCAA TGTCACCAGC AAATGGAAAG GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA 
AAATCGTCTC TGATCTCTTC TATGTCCATC TCATCATCGT CCACTTCTCT CTCTTCTAAT 
ACTTCTACCG AAGGAAGTGG GACCATGAAG AAACTGGATA CAACCCTGGC CTCGGCCCTT 
GCCCCTCCCC CTCCTCCTCT TCCCCCTCTG CCTTCTCCGT GTCTGGCAGA CAAGTCCCCT 
TTTCTTCCAC CACCTCCTCC TCTAGCAGAT TGCTCCGAGG GCTCTCCTCT GCCGCCCTCT 
CCCATGTTCC CCCCTCCACC ACCAGAAGCC CTTGTTCCCT TCTGCTCCCC CACCGACGGG 
TGCCTTTCTC CTTCTCCCAC AGCAGTTAGC CCCTCTCTTC CAAGATCCTT GCCTCCTGTG 
CCAGCACCTC CACCTTTCTT GCCCTCATCA GAACCCCCGC CCGCTCCGCC TCTGGACCCC 
AAACTCATGA AAGAAAACAG GCCCTTTTTC AAAAACTCTA GCCAGTCCGA ATCCTCTAGG 
GAGGCCCTCA GGCGGCCTGC CAACAAGGAG GAGGGCTGCC GACCCCCCAT GCCCCTGATC 
ACCACAGAAG CTTTGCAGAT GGTGCAGTTG AGGCCAGTGA GGAAGAACTC AGGTGCCGGG 
GCAGTCCTGT TTTCTGAACC ATCAGCTCAG GAACAACGAA CTCCGACTGC TCCACAGTAT 
CACTTAAAGC CATCTGCTTT CCTCAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA 
AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT CCGATGCCTG CCAAGAGCCA GAGTCAGGGT 
GACCATGACA GTGCAGTAGA GCGTGGCGGC CTTCCGAGCT GCAGCGATGG AGCTCCAGGC 
CCGGGTCCCA GCCTCAGGAC CACTCTGCTC CCAGACTCTT CACCCAGCAG GAAGCCACCC 
CCCATCTCTA AGAAGCCCAA ACTGTTCCTG GTGGTACCAC CTCCGCAGAG AGATTTCACA 
GCGGAGCCCA CAGAGAACGG GAGCGAAGCT TTCCCAGGCG TGCCCAGCCC TACCAGGGCA 
GAGGGGGAAG CTGTGCGGAG CCAAGAGGAG AAATCCAGCC CAGCATCCCG AGCTGGCTCT 
CATGCTACGG CCCCCACCCC CGGCAGTCCG GCTCTAGAAC CGGGAACTGC AGGGTCCTTA 
TCCTCTAGCA TTGTGGAAGC CAATGTCCCC ATGGTCCAAC CTAATACATC ACCGGGCCCC 
ACACAGGAAG AGTCAGGCGA GAACAGTGTG GATGGTGAGA GAAATGCGAA GAGCTGCCTG 
TCTCAACAGG GCAGAGAAGC TGGCTTGCTG GAACCCAACA CAGCTGCTTC CTCTTCAGAC 
CCCGTGGATG TATCTAAGGA AGAGGGAAGC GATGAGGTGC TGACTCCGAC TAAACCCAGG 
ACAACAGAAG ACCTGTTTGC AGCCATTCAC AGATCTAAAA GGAAAGTCCT TGGCCGGAAA 
GACTCAGAAG ACGATCACAC TCGAAATCAC TCTCCCTCCC CGCCTGTGAC ACCCACGAGT 
GCTGCCCCTA ACCTGGCCTC TCCAAAGCAA GTAGGGTCTA TCCAGAGAAG CATAAAAAAG 
AGCACTACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AGAAGGGGAG TCGCTCGGAC 
ACCAGCGCTC GCATGTCAGC GGCTGAGATG CTCAAGTCTA CGGACCCTCG CTTCCAGAGA 
TCTAGGTCGG AGCCTTCGGC AGACAGCCCG GACAGCCCAT CCAGCTGCTC CCCCAACAAG 
AACAGGAGAG CTCAGGAAGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC 
TTTTCGGGCC CTCGGTACAG CCGCAGCAGG ACGCCACCCT CTGCGGCCAG CAGCAGGTAC 
AGCATGCGGA ACCGCATCCA GAGCAGCCCC ATGACCGTCA TCTCCGAGGG AGAGGGAGAA 
CCTGCTGAGC CAGCGGACAA CAAAGCACGC AGGGCCCTGG ATGCTACCAG GGTGTGCTCT 
CTGGACAGAC TGACGGGTCA GGAGATGGAC CAGGCCAGCC TCCTCTGTAG CGAGGAGCCT 
GCCTCTGTGG ATGGGATAGG AAGGGCTGAG GGCAACGGTC CCTCGGAGCA GTGTGGGGGT 
ACGGAACAGA AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100865.1
CDS	5894..10471
Translation	MFCLKAVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_030245005.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245005.1

ORF Insert Sequence:

ATGTTTTGCC TGAAAGCGGT TTCCAACTTG GACGAGGAGA GTCGATGGAC TGTCCACTAC 
ACGGCACCAT GGCATCAGCA AGAGAATGTG TTCCTTCCCG CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG 
CTGAGGCAAG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC 
GGCTCCAGCC CCTGCGACGA TTATCAAGAT GAAGACACAG AAGCAGACCG AAAGTGCTCT 
CTCTCTTCAT CTGAAGAAGA AAGATTTATC GGAATCAGGA GACCTAAAAC GCCAACCTCA 
GGTGACTTCT CCGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA 
CCAGAAGAGA AGACGCGGCA GCAAGCCCAG ACGGTCCAGG CTGACGTGGT TCCTATAAAC 
ATAACTGCAT CAGCCACTGG CCAAGATGAT GATGGTAGTG CCCATTCGCT GTACGTCCCA 
GACCACTACT CTACGTTAGG AAGGCTTGAT AGTTATCGGT CCACCGGGCA ATGCTTGGAA 
ACCAGGGACA CCAGCTGTCA GACCGAAGAC GTGAAGGTCA TCCCACCATC AATGAGAAGG 
ATCAGGGCTC ACAAGGGGGT AGGCGTTGCT GCCCAGATGA GCCACCTCTC AGGTTCCTCA 
GGCAACATGT CTGTGCTGAG TGACTCAGCC GGTGTTGTGT TCCCATCTCG CCTCAGTAAC 
GATACTGGTT TCCACAGTCT TCCACGTACC GGCCCGAGAG CAAGCACCTA TTCACTGGAG 
GGAAGGATGG GTGCCCTGGG CTCTACTGAA GATACGGATG ACACTTCTCC CTACCAGGGG 
GGTAGCTTAC AGGGGCATGA AAACTTTGCG CATTTAGGAG GTGCGTCGAG TACTGGGATG 
CTTTCGAGGC CCAAATCCCA GCAGTTGAGG TTTTTGGAGA GTCCAGCATG TGTGGTTTCA 
CCTCATGCAG CCTACTCTAC CAGTGTCATC CCAAATGCCA CGCTACTGTC CTCTTCAGAG 
GTTATTGTCA TTCACACTGC TCAGAGTGCA GGACAGCTGG ACAGTAGAAC ACCCGGTTCA 
TCTTCATATT CAAAAATAAA ACCCAGAGAC CGTCCCACAC CCAGATGTTC TGTGAAAGAT 
GACCATCAGT CCCCCCGTCA TCACTGGAAC GAGGGTCATC TCATTCATTC ACGGGCTTTA 
GCCTCCTCTG TCCCTGGTGC CACCACGCTG TTATCCCTCC ATGATTCAGA GGTCTCTCTA 
AATGCCCCAG CAAACAGGGA AAATGGATCC CAAGCCATAC TCTATCATTG TAGAAACAAC 
CCAAGCTTTC CTGACCACCC TTCAGATGTG GATGGCAAGA GCGAGTGTAG TTATTCGGGG 
GACAGGGGAT GTGGCAGTTC TGAGCCCTGG GAATATAAAA CCTCCAGCAA TGGGCGGGCA 
TCCCCACTGA AGCCACACCT GGCCACTCCT GGTTGCTCTA CTCCCACAAG TAACGTGAGC 
AGCTGCAGTT TGGACCAAAC ATCTCTCAAG GGTGATACCA GGTCCCTGTG TTCAGAGGAC 
CATGATGGTT ACTATACTAC CACTCACGAG GCTGGGAACC TGTACACTCT CAGTGATGGC 
TTGGGGAACC CCAGGCACAG CATGGTCAAT GTTTTTGATG GAAGGGCTCA GAGAAGCCAA 
GGGGATCAGG CCGCTCACCA GGATAAAATC CTTTCGAGAA ACATCTCTCT GAAGAAAGCA 
AAGAAACCAC CCCTGCCACC ATCCCGGACC GATTCTCTGC GAAGGATTCC CAAAAAGAAC 
AACCAGACAA ACGGTCAGGT GCTCAATGAG AGCCTGATCG CCTCGCTCCA GCATTCACTG 
CAGCTAAGCC TCCCTGGCAA GGGCGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTTT 
GAGGAACCCT GGCTCCCCAG GTCCAGAAGC CAGAGCATTG TTAGCGAGGG TAGTAGCTTG 
ACCTCCACCA CCACTCCCAA CGTGTATTCT CTGTGTGGGG TCACTCCGTC CCAGAGCGAC 
ACAAGTAGCG TCAAGTCGGA ATACACGGAC CCTTGGGGTT ACTACATTGA CTACACGAGC 
TTGCAGGAAG ATCCAGGGAA CCCCACCGGG GGCTGCTCAG CCAACACTGA GGCAGCAACT 
GGAAACGGGC CAGTACGCCA CATCCAGGAG GGGTCGAGAG TCCCAGTGCC CCAAGTGCCG 
GGCTGCTCAG TTAGACCAAA GATCGCCTCA CCAGAGAAGT CACAGAGAGT CACCTCTCCA 
TCCAGTGGGT ATTCTAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT 
TTAAAATCAA TGTCACCAGC AAATGGAAAG GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA 
AAATCGTCTC TGATCTCTTC TATGTCCATC TCATCATCGT CCACTTCTCT CTCTTCTAAT 
ACTTCTACCG AAGGAAGTGG GACCATGAAG AAACTGGATA CAACCCTGGC CTCGGCCCTT 
GCCCCTCCCC CTCCTCCTCT TCCCCCTCTG CCTTCTCCGT GTCTGGCAGA CAAGTCCCCT 
TTTCTTCCAC CACCTCCTCC TCTAGCAGAT TGCTCCGAGG GCTCTCCTCT GCCGCCCTCT 
CCCATGTTCC CCCCTCCACC ACCAGAAGCC CTTGTTCCCT TCTGCTCCCC CACCGACGGG 
TGCCTTTCTC CTTCTCCCAC AGCAGTTAGC CCCTCTCTTC CAAGATCCTT GCCTCCTGTG 
CCAGCACCTC CACCTTTCTT GCCCTCATCA GAACCCCCGC CCGCTCCGCC TCTGGACCCC 
AAACTCATGA AAGAAAACAG GCCCTTTTTC AAAAACTCTA GCCAGTCCGA ATCCTCTAGG 
GAGGCCCTCA GGCGGCCTGC CAACAAGGAG GAGGGCTGCC GACCCCCCAT GCCCCTGATC 
ACCACAGAAG CTTTGCAGAT GGTGCAGTTG AGGCCAGTGA GGAAGAACTC AGGTGCCGGG 
GCAGTCCTGT TTTCTGAACC ATCAGCTCAG GAACAACGAA CTCCGACTGC TCCACAGTAT 
CACTTAAAGC CATCTGCTTT CCTCAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA 
AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT CCGATGCCTG CCAAGAGCCA GAGTCAGGGT 
GACCATGACA GTGCAGTAGA GCGTGGCGGC CTTCCGAGCT GCAGCGATGG AGCTCCAGGC 
CCGGGTCCCA GCCTCAGGAC CACTCTGCTC CCAGACTCTT CACCCAGCAG GAAGCCACCC 
CCCATCTCTA AGAAGCCCAA ACTGTTCCTG GTGGTACCAC CTCCGCAGAG AGATTTCACA 
GCGGAGCCCA CAGAGAACGG GAGCGAAGCT TTCCCAGGCG TGCCCAGCCC TACCAGGGCA 
GAGGGGGAAG CTGTGCGGAG CCAAGAGGAG AAATCCAGCC CAGCATCCCG AGCTGGCTCT 
CATGCTACGG CCCCCACCCC CGGCAGTCCG GCTCTAGAAC CGGGAACTGC AGGGTCCTTA 
TCCTCTAGCA TTGTGGAAGC CAATGTCCCC ATGGTCCAAC CTAATACATC ACCGGGCCCC 
ACACAGGAAG AGTCAGGCGA GAACAGTGTG GATGGTGAGA GAAATGCGAA GAGCTGCCTG 
TCTCAACAGG GCAGAGAAGC TGGCTTGCTG GAACCCAACA CAGCTGCTTC CTCTTCAGAC 
CCCGTGGATG TATCTAAGGA AGAGGGAAGC GATGAGGTGC TGACTCCGAC TAAACCCAGG 
ACAACAGAAG ACCTGTTTGC AGCCATTCAC AGATCTAAAA GGAAAGTCCT TGGCCGGAAA 
GACTCAGAAG ACGATCACAC TCGAAATCAC TCTCCCTCCC CGCCTGTGAC ACCCACGAGT 
GCTGCCCCTA ACCTGGCCTC TCCAAAGCAA GTAGGGTCTA TCCAGAGAAG CATAAAAAAG 
AGCACTACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AGAAGGGGAG TCGCTCGGAC 
ACCAGCGCTC GCATGTCAGC GGCTGAGATG CTCAAGTCTA CGGACCCTCG CTTCCAGAGA 
TCTAGGTCGG AGCCTTCGGC AGACAGCCCG GACAGCCCAT CCAGCTGCTC CCCCAACAAG 
AACAGGAGAG CTCAGGAAGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC 
TTTTCGGGCC CTCGGTACAG CCGCAGCAGG ACGCCACCCT CTGCGGCCAG CAGCAGGTAC 
AGCATGCGGA ACCGCATCCA GAGCAGCCCC ATGACCGTCA TCTCCGAGGG AGAGGGAGAA 
CCTGCTGAGC CAGCGGACAA CAAAGCACGC AGGGCCCTGG ATGCTACCAG GGTGTGCTCT 
CTGGACAGAC TGACGGGTCA GGAGATGGAC CAGGCCAGCC TCCTCTGTAG CGAGGAGCCT 
GCCTCTGTGG ATGGGATAGG AAGGGCTGAG GGCAACGGTC CCTCGGAGCA GTGTGGGGGT 
ACGGAACAGA AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( NM_173390.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_775566.3 Mus musculus NHS-like 1 (Nhsl1), transcript variant 1, mRNA.

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CloneID	OMu04541
Clone ID Related Accession (Same CDS sequence)	NM_173390.3
Accession Version	NM_173390.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY717318.1, AK038892.1, AC153561.5, AK043447.1 and AW488074.1. On Feb 2, 2007 this sequence version replaced NM_173390.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAAAGG ACGGCTCCTC AGGTTCCTTC GGAATCAAGG CAAGCCCAGG CTCCCTCTCA 
CGTGCTGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTCCGC 
AGTGATGGAG AGCTGTCCGT TTGTGGGCAC CAGGTGGAAG CCGATGATGA GAACTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTGGACG AGGAGAGTCG ATGGACTGTC 
CACTACACGG CACCATGGCA TCAGCAAGAG AATGTGTTCC TTCCCGCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCAAGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCGGCT CCAGCCCCTG CGACGATTAT CAAGATGAAG ACACAGAAGC AGACCGAAAG 
TGCTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATCGGAA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCCGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC GCGGCAGCAA GCCCAGACGG TCCAGGCTGA CGTGGTTCCT 
ATAAACATAA CTGCATCAGC CACTGGCCAA GATGATGATG GTAGTGCCCA TTCGCTGTAC 
GTCCCAGACC ACTACTCTAC GTTAGGAAGG CTTGATAGTT ATCGGTCCAC CGGGCAATGC 
TTGGAAACCA GGGACACCAG CTGTCAGACC GAAGACGTGA AGGTCATCCC ACCATCAATG 
AGAAGGATCA GGGCTCACAA GGGGGTAGGC GTTGCTGCCC AGATGAGCCA CCTCTCAGGT 
TCCTCAGGCA ACATGTCTGT GCTGAGTGAC TCAGCCGGTG TTGTGTTCCC ATCTCGCCTC 
AGTAACGATA CTGGTTTCCA CAGTCTTCCA CGTACCGGCC CGAGAGCAAG CACCTATTCA 
CTGGAGGGAA GGATGGGTGC CCTGGGCTCT ACTGAAGATA CGGATGACAC TTCTCCCTAC 
CAGGGGGGTA GCTTACAGGG GCATGAAAAC TTTGCGCATT TAGGAGGTGC GTCGAGTACT 
GGGATGCTTT CGAGGCCCAA ATCCCAGCAG TTGAGGTTTT TGGAGAGTCC AGCATGTGTG 
GTTTCACCTC ATGCAGCCTA CTCTACCAGT GTCATCCCAA ATGCCACGCT ACTGTCCTCT 
TCAGAGGTTA TTGTCATTCA CACTGCTCAG AGTGCAGGAC AGCTGGACAG TAGAACACCC 
GGTTCATCTT CATATTCAAA AATAAAACCC AGAGACCGTC CCACACCCAG ATGTTCTGTG 
AAAGATGACC ATCAGTCCCC CCGTCATCAC TGGAACGAGG GTCATCTCAT TCATTCACGG 
GCTTTAGCCT CCTCTGTCCC TGGTGCCACC ACGCTGTTAT CCCTCCATGA TTCAGAGGTC 
TCTCTAAATG CCCCAGCAAA CAGGGAAAAT GGATCCCAAG CCATACTCTA TCATTGTAGA 
AACAACCCAA GCTTTCCTGA CCACCCTTCA GATGTGGATG GCAAGAGCGA GTGTAGTTAT 
TCGGGGGACA GGGGATGTGG CAGTTCTGAG CCCTGGGAAT ATAAAACCTC CAGCAATGGG 
CGGGCATCCC CACTGAAGCC ACACCTGGCC ACTCCTGGTT GCTCTACTCC CACAAGTAAC 
GTGAGCAGCT GCAGTTTGGA CCAAACATCT CTCAAGGGTG ATACCAGGTC CCTGTGTTCA 
GAGGACCATG ATGGTTACTA TACTACCACT CACGAGGCTG GGAACCTGTA CACTCTCAGT 
GATGGCTTGG GGAACCCCAG GCACAGCATG GTCAATGTTT TTGATGGAAG GGCTCAGAGA 
AGCCAAGGGG ATCAGGCCGC TCACCAGGAT AAAATCCTTT CGAGAAACAT CTCTCTGAAG 
AAAGCAAAGA AACCACCCCT GCCACCATCC CGGACCGATT CTCTGCGAAG GATTCCCAAA 
AAGAACAACC AGACAAACGG TCAGGTGCTC AATGAGAGCC TGATCGCCTC GCTCCAGCAT 
TCACTGCAGC TAAGCCTCCC TGGCAAGGGC GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT 
GACTTTGAGG AACCCTGGCT CCCCAGGTCC AGAAGCCAGA GCATTGTTAG CGAGGGTAGT 
AGCTTGACCT CCACCACCAC TCCCAACGTG TATTCTCTGT GTGGGGTCAC TCCGTCCCAG 
AGCGACACAA GTAGCGTCAA GTCGGAATAC ACGGACCCTT GGGGTTACTA CATTGACTAC 
ACGAGCTTGC AGGAAGATCC AGGGAACCCC ACCGGGGGCT GCTCAGCCAA CACTGAGGCA 
GCAACTGGAA ACGGGCCAGT ACGCCACATC CAGGAGGGGT CGAGAGTCCC AGTGCCCCAA 
GTGCCGGGCT GCTCAGTTAG ACCAAAGATC GCCTCACCAG AGAAGTCACA GAGAGTCACC 
TCTCCATCCA GTGGGTATTC TAGCCAGTCG AATACACCCA CAGCACTCAC CCCTGTGCCT 
GTGTTTTTAA AATCAATGTC ACCAGCAAAT GGAAAGGGGA AGGCCAAGCC CAAGGTACCA 
GAAAGAAAAT CGTCTCTGAT CTCTTCTATG TCCATCTCAT CATCGTCCAC TTCTCTCTCT 
TCTAATACTT CTACCGAAGG AAGTGGGACC ATGAAGAAAC TGGATACAAC CCTGGCCTCG 
GCCCTTGCCC CTCCCCCTCC TCCTCTTCCC CCTCTGCCTT CTCCGTGTCT GGCAGACAAG 
TCCCCTTTTC TTCCACCACC TCCTCCTCTA GCAGATTGCT CCGAGGGCTC TCCTCTGCCG 
CCCTCTCCCA TGTTCCCCCC TCCACCACCA GAAGCCCTTG TTCCCTTCTG CTCCCCCACC 
GACGGGTGCC TTTCTCCTTC TCCCACAGCA GTTAGCCCCT CTCTTCCAAG ATCCTTGCCT 
CCTGTGCCAG CACCTCCACC TTTCTTGCCC TCATCAGAAC CCCCGCCCGC TCCGCCTCTG 
GACCCCAAAC TCATGAAAGA AAACAGGCCC TTTTTCAAAA ACTCTAGCCA GTCCGAATCC 
TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC AAGGAGGAGG GCTGCCGACC CCCCATGCCC 
CTGATCACCA CAGAAGCTTT GCAGATGGTG CAGTTGAGGC CAGTGAGGAA GAACTCAGGT 
GCCGGGGCAG TCCTGTTTTC TGAACCATCA GCTCAGGAAC AACGAACTCC GACTGCTCCA 
CAGTATCACT TAAAGCCATC TGCTTTCCTC AAATCCCGAA ATAGCATAAA TGAAATGGAG 
AGTGAAAGCC AGGCTGCCTC TGTGACGAGC TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT 
CAGGGTGACC ATGACAGTGC AGTAGAGCGT GGCGGCCTTC CGAGCTGCAG CGATGGAGCT 
CCAGGCCCGG GTCCCAGCCT CAGGACCACT CTGCTCCCAG ACTCTTCACC CAGCAGGAAG 
CCACCCCCCA TCTCTAAGAA GCCCAAACTG TTCCTGGTGG TACCACCTCC GCAGAGAGAT 
TTCACAGCGG AGCCCACAGA GAACGGGAGC GAAGCTTTCC CAGGCGTGCC CAGCCCTACC 
AGGGCAGAGG GGGAAGCTGT GCGGAGCCAA GAGGAGAAAT CCAGCCCAGC ATCCCGAGCT 
GGCTCTCATG CTACGGCCCC CACCCCCGGC AGTCCGGCTC TAGAACCGGG AACTGCAGGG 
TCCTTATCCT CTAGCATTGT GGAAGCCAAT GTCCCCATGG TCCAACCTAA TACATCACCG 
GGCCCCACAC AGGAAGAGTC AGGCGAGAAC AGTGTGGATG GTGAGAGAAA TGCGAAGAGC 
TGCCTGTCTC AACAGGGCAG AGAAGCTGGC TTGCTGGAAC CCAACACAGC TGCTTCCTCT 
TCAGACCCCG TGGATGTATC TAAGGAAGAG GGAAGCGATG AGGTGCTGAC TCCGACTAAA 
CCCAGGACAA CAGAAGACCT GTTTGCAGCC ATTCACAGAT CTAAAAGGAA AGTCCTTGGC 
CGGAAAGACT CAGAAGACGA TCACACTCGA AATCACTCTC CCTCCCCGCC TGTGACACCC 
ACGAGTGCTG CCCCTAACCT GGCCTCTCCA AAGCAAGTAG GGTCTATCCA GAGAAGCATA 
AAAAAGAGCA CTACCAGCAG TGACAACTTC AAAGCTCTGC TGCTAAAGAA GGGGAGTCGC 
TCGGACACCA GCGCTCGCAT GTCAGCGGCT GAGATGCTCA AGTCTACGGA CCCTCGCTTC 
CAGAGATCTA GGTCGGAGCC TTCGGCAGAC AGCCCGGACA GCCCATCCAG CTGCTCCCCC 
AACAAGAACA GGAGAGCTCA GGAAGAGTGG GCCAAGAACG AAGGCCTGAT GCCTCGGAGT 
CTGTCCTTTT CGGGCCCTCG GTACAGCCGC AGCAGGACGC CACCCTCTGC GGCCAGCAGC 
AGGTACAGCA TGCGGAACCG CATCCAGAGC AGCCCCATGA CCGTCATCTC CGAGGGAGAG 
GGAGAACCTG CTGAGCCAGC GGACAACAAA GCACGCAGGG CCCTGGATGC TACCAGGGTG 
TGCTCTCTGG ACAGACTGAC GGGTCAGGAG ATGGACCAGG CCAGCCTCCT CTGTAGCGAG 
GAGCCTGCCT CTGTGGATGG GATAGGAAGG GCTGAGGGCA ACGGTCCCTC GGAGCAGTGT 
GGGGGTACGG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775566.3
CDS	639..5402
Translation	MKKDGSSGSFGIKASPGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGHQVEADDENWIYRTQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	NM_173390.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173390.3

ORF Insert Sequence:

ATGAAAAAGG ACGGCTCCTC AGGTTCCTTC GGAATCAAGG CAAGCCCAGG CTCCCTCTCA 
CGTGCTGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTCCGC 
AGTGATGGAG AGCTGTCCGT TTGTGGGCAC CAGGTGGAAG CCGATGATGA GAACTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTGGACG AGGAGAGTCG ATGGACTGTC 
CACTACACGG CACCATGGCA TCAGCAAGAG AATGTGTTCC TTCCCGCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCAAGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCGGCT CCAGCCCCTG CGACGATTAT CAAGATGAAG ACACAGAAGC AGACCGAAAG 
TGCTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATCGGAA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCCGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC GCGGCAGCAA GCCCAGACGG TCCAGGCTGA CGTGGTTCCT 
ATAAACATAA CTGCATCAGC CACTGGCCAA GATGATGATG GTAGTGCCCA TTCGCTGTAC 
GTCCCAGACC ACTACTCTAC GTTAGGAAGG CTTGATAGTT ATCGGTCCAC CGGGCAATGC 
TTGGAAACCA GGGACACCAG CTGTCAGACC GAAGACGTGA AGGTCATCCC ACCATCAATG 
AGAAGGATCA GGGCTCACAA GGGGGTAGGC GTTGCTGCCC AGATGAGCCA CCTCTCAGGT 
TCCTCAGGCA ACATGTCTGT GCTGAGTGAC TCAGCCGGTG TTGTGTTCCC ATCTCGCCTC 
AGTAACGATA CTGGTTTCCA CAGTCTTCCA CGTACCGGCC CGAGAGCAAG CACCTATTCA 
CTGGAGGGAA GGATGGGTGC CCTGGGCTCT ACTGAAGATA CGGATGACAC TTCTCCCTAC 
CAGGGGGGTA GCTTACAGGG GCATGAAAAC TTTGCGCATT TAGGAGGTGC GTCGAGTACT 
GGGATGCTTT CGAGGCCCAA ATCCCAGCAG TTGAGGTTTT TGGAGAGTCC AGCATGTGTG 
GTTTCACCTC ATGCAGCCTA CTCTACCAGT GTCATCCCAA ATGCCACGCT ACTGTCCTCT 
TCAGAGGTTA TTGTCATTCA CACTGCTCAG AGTGCAGGAC AGCTGGACAG TAGAACACCC 
GGTTCATCTT CATATTCAAA AATAAAACCC AGAGACCGTC CCACACCCAG ATGTTCTGTG 
AAAGATGACC ATCAGTCCCC CCGTCATCAC TGGAACGAGG GTCATCTCAT TCATTCACGG 
GCTTTAGCCT CCTCTGTCCC TGGTGCCACC ACGCTGTTAT CCCTCCATGA TTCAGAGGTC 
TCTCTAAATG CCCCAGCAAA CAGGGAAAAT GGATCCCAAG CCATACTCTA TCATTGTAGA 
AACAACCCAA GCTTTCCTGA CCACCCTTCA GATGTGGATG GCAAGAGCGA GTGTAGTTAT 
TCGGGGGACA GGGGATGTGG CAGTTCTGAG CCCTGGGAAT ATAAAACCTC CAGCAATGGG 
CGGGCATCCC CACTGAAGCC ACACCTGGCC ACTCCTGGTT GCTCTACTCC CACAAGTAAC 
GTGAGCAGCT GCAGTTTGGA CCAAACATCT CTCAAGGGTG ATACCAGGTC CCTGTGTTCA 
GAGGACCATG ATGGTTACTA TACTACCACT CACGAGGCTG GGAACCTGTA CACTCTCAGT 
GATGGCTTGG GGAACCCCAG GCACAGCATG GTCAATGTTT TTGATGGAAG GGCTCAGAGA 
AGCCAAGGGG ATCAGGCCGC TCACCAGGAT AAAATCCTTT CGAGAAACAT CTCTCTGAAG 
AAAGCAAAGA AACCACCCCT GCCACCATCC CGGACCGATT CTCTGCGAAG GATTCCCAAA 
AAGAACAACC AGACAAACGG TCAGGTGCTC AATGAGAGCC TGATCGCCTC GCTCCAGCAT 
TCACTGCAGC TAAGCCTCCC TGGCAAGGGC GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT 
GACTTTGAGG AACCCTGGCT CCCCAGGTCC AGAAGCCAGA GCATTGTTAG CGAGGGTAGT 
AGCTTGACCT CCACCACCAC TCCCAACGTG TATTCTCTGT GTGGGGTCAC TCCGTCCCAG 
AGCGACACAA GTAGCGTCAA GTCGGAATAC ACGGACCCTT GGGGTTACTA CATTGACTAC 
ACGAGCTTGC AGGAAGATCC AGGGAACCCC ACCGGGGGCT GCTCAGCCAA CACTGAGGCA 
GCAACTGGAA ACGGGCCAGT ACGCCACATC CAGGAGGGGT CGAGAGTCCC AGTGCCCCAA 
GTGCCGGGCT GCTCAGTTAG ACCAAAGATC GCCTCACCAG AGAAGTCACA GAGAGTCACC 
TCTCCATCCA GTGGGTATTC TAGCCAGTCG AATACACCCA CAGCACTCAC CCCTGTGCCT 
GTGTTTTTAA AATCAATGTC ACCAGCAAAT GGAAAGGGGA AGGCCAAGCC CAAGGTACCA 
GAAAGAAAAT CGTCTCTGAT CTCTTCTATG TCCATCTCAT CATCGTCCAC TTCTCTCTCT 
TCTAATACTT CTACCGAAGG AAGTGGGACC ATGAAGAAAC TGGATACAAC CCTGGCCTCG 
GCCCTTGCCC CTCCCCCTCC TCCTCTTCCC CCTCTGCCTT CTCCGTGTCT GGCAGACAAG 
TCCCCTTTTC TTCCACCACC TCCTCCTCTA GCAGATTGCT CCGAGGGCTC TCCTCTGCCG 
CCCTCTCCCA TGTTCCCCCC TCCACCACCA GAAGCCCTTG TTCCCTTCTG CTCCCCCACC 
GACGGGTGCC TTTCTCCTTC TCCCACAGCA GTTAGCCCCT CTCTTCCAAG ATCCTTGCCT 
CCTGTGCCAG CACCTCCACC TTTCTTGCCC TCATCAGAAC CCCCGCCCGC TCCGCCTCTG 
GACCCCAAAC TCATGAAAGA AAACAGGCCC TTTTTCAAAA ACTCTAGCCA GTCCGAATCC 
TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC AAGGAGGAGG GCTGCCGACC CCCCATGCCC 
CTGATCACCA CAGAAGCTTT GCAGATGGTG CAGTTGAGGC CAGTGAGGAA GAACTCAGGT 
GCCGGGGCAG TCCTGTTTTC TGAACCATCA GCTCAGGAAC AACGAACTCC GACTGCTCCA 
CAGTATCACT TAAAGCCATC TGCTTTCCTC AAATCCCGAA ATAGCATAAA TGAAATGGAG 
AGTGAAAGCC AGGCTGCCTC TGTGACGAGC TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT 
CAGGGTGACC ATGACAGTGC AGTAGAGCGT GGCGGCCTTC CGAGCTGCAG CGATGGAGCT 
CCAGGCCCGG GTCCCAGCCT CAGGACCACT CTGCTCCCAG ACTCTTCACC CAGCAGGAAG 
CCACCCCCCA TCTCTAAGAA GCCCAAACTG TTCCTGGTGG TACCACCTCC GCAGAGAGAT 
TTCACAGCGG AGCCCACAGA GAACGGGAGC GAAGCTTTCC CAGGCGTGCC CAGCCCTACC 
AGGGCAGAGG GGGAAGCTGT GCGGAGCCAA GAGGAGAAAT CCAGCCCAGC ATCCCGAGCT 
GGCTCTCATG CTACGGCCCC CACCCCCGGC AGTCCGGCTC TAGAACCGGG AACTGCAGGG 
TCCTTATCCT CTAGCATTGT GGAAGCCAAT GTCCCCATGG TCCAACCTAA TACATCACCG 
GGCCCCACAC AGGAAGAGTC AGGCGAGAAC AGTGTGGATG GTGAGAGAAA TGCGAAGAGC 
TGCCTGTCTC AACAGGGCAG AGAAGCTGGC TTGCTGGAAC CCAACACAGC TGCTTCCTCT 
TCAGACCCCG TGGATGTATC TAAGGAAGAG GGAAGCGATG AGGTGCTGAC TCCGACTAAA 
CCCAGGACAA CAGAAGACCT GTTTGCAGCC ATTCACAGAT CTAAAAGGAA AGTCCTTGGC 
CGGAAAGACT CAGAAGACGA TCACACTCGA AATCACTCTC CCTCCCCGCC TGTGACACCC 
ACGAGTGCTG CCCCTAACCT GGCCTCTCCA AAGCAAGTAG GGTCTATCCA GAGAAGCATA 
AAAAAGAGCA CTACCAGCAG TGACAACTTC AAAGCTCTGC TGCTAAAGAA GGGGAGTCGC 
TCGGACACCA GCGCTCGCAT GTCAGCGGCT GAGATGCTCA AGTCTACGGA CCCTCGCTTC 
CAGAGATCTA GGTCGGAGCC TTCGGCAGAC AGCCCGGACA GCCCATCCAG CTGCTCCCCC 
AACAAGAACA GGAGAGCTCA GGAAGAGTGG GCCAAGAACG AAGGCCTGAT GCCTCGGAGT 
CTGTCCTTTT CGGGCCCTCG GTACAGCCGC AGCAGGACGC CACCCTCTGC GGCCAGCAGC 
AGGTACAGCA TGCGGAACCG CATCCAGAGC AGCCCCATGA CCGTCATCTC CGAGGGAGAG 
GGAGAACCTG CTGAGCCAGC GGACAACAAA GCACGCAGGG CCCTGGATGC TACCAGGGTG 
TGCTCTCTGG ACAGACTGAC GGGTCAGGAG ATGGACCAGG CCAGCCTCCT CTGTAGCGAG 
GAGCCTGCCT CTGTGGATGG GATAGGAAGG GCTGAGGGCA ACGGTCCCTC GGAGCAGTGT 
GGGGGTACGG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( NM_001163592.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157064.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant 2, mRNA.

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CloneID	OMu09232
Clone ID Related Accession (Same CDS sequence)	NM_001163592.1
Accession Version	NM_001163592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153561.5 and AW488074.1. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, uses a distinct start codon, and includes an alternate in-frame exon, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus and includes an alternate segment, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGTCT TCATTAATGC AAAGATTAAA TCTTTCTTTA AACTTTTTAA AAAGAAAACG 
GTTTCCAACT TGGACGAGGA GAGTCGATGG ACTGTCCACT ACACGGCACC ATGGCATCAG 
CAAGAGAATG TGTTCCTTCC CGCCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCCAAGCTCA ACCTCAAATC AGTGCTGAGG GAATGTGACA AGCTGAGGCA AGATGGCTGC 
CGAAGTTCTC AGTACTATTC TCAGGGACCC ACCTTTGCAG CCGGCTCCAG CCCCTGCGAC 
GATTATCAAG ATGAAGACAC AGAAGCAGAC CGAAAGTGCT CTCTCTCTTC ATCTGAAGAA 
GAAAGATTTA TCGGAATCAG GAGACCTAAA ACGCCAACCT CAGGTGACTT CTCCGACCTT 
CATACTCAGA CAAACTGGAC CAAGTCCCTG CCGCTGCCAA CACCAGAAGA GAAGACGCGG 
CAGCAAGCCC AGACGGTCCA GGCTGACGTG GTTCCTATAA ACATAACTGG GGAGAATTTC 
GATCGTCAGG CCAGCCTTCG GCGGTCTCTA ATTTACACAG ACACTCTGGT AAGACGACCG 
AAGAAAGTCA AAAGGAGAAA GACAATTTCA GGAATCCCTG ACATCATTCA GAAGGAGCTA 
GCATCAGCCA CTGGCCAAGA TGATGATGGT AGTGCCCATT CGCTGTACGT CCCAGACCAC 
TACTCTACGT TAGGAAGGCT TGATAGTTAT CGGTCCACCG GGCAATGCTT GGAAACCAGG 
GACACCAGCT GTCAGACCGA AGACGTGAAG GTCATCCCAC CATCAATGAG AAGGATCAGG 
GCTCACAAGG GGGTAGGCGT TGCTGCCCAG ATGAGCCACC TCTCAGGTTC CTCAGGCAAC 
ATGTCTGTGC TGAGTGACTC AGCCGGTGTT GTGTTCCCAT CTCGCCTCAG TAACGATACT 
GGTTTCCACA GTCTTCCACG TACCGGCCCG AGAGCAAGCA CCTATTCACT GGAGGGAAGG 
ATGGGTGCCC TGGGCTCTAC TGAAGATACG GATGACACTT CTCCCTACCA GGGGGGTAGC 
TTACAGGGGC ATGAAAACTT TGCGCATTTA GGAGGTGCGT CGAGTACTGG GATGCTTTCG 
AGGCCCAAAT CCCAGCAGTT GAGGTTTTTG GAGAGTCCAG CATGTGTGGT TTCACCTCAT 
GCAGCCTACT CTACCAGTGT CATCCCAAAT GCCACGCTAC TGTCCTCTTC AGAGGTTATT 
GTCATTCACA CTGCTCAGAG TGCAGGACAG CTGGACAGTA GAACACCCGG TTCATCTTCA 
TATTCAAAAA TAAAACCCAG AGACCGTCCC ACACCCAGAT GTTCTGTGAA AGATGACCAT 
CAGTCCCCCC GTCATCACTG GAACGAGGGT CATCTCATTC ATTCACGGGC TTTAGCCTCC 
TCTGTCCCTG GTGCCACCAC GCTGTTATCC CTCCATGATT CAGAGGTCTC TCTAAATGCC 
CCAGCAAACA GGGAAAATGG ATCCCAAGCC ATACTCTATC ATTGTAGAAA CAACCCAAGC 
TTTCCTGACC ACCCTTCAGA TGTGGATGGC AAGAGCGAGT GTAGTTATTC GGGGGACAGG 
GGATGTGGCA GTTCTGAGCC CTGGGAATAT AAAACCTCCA GCAATGGGCG GGCATCCCCA 
CTGAAGCCAC ACCTGGCCAC TCCTGGTTGC TCTACTCCCA CAAGTAACGT GAGCAGCTGC 
AGTTTGGACC AAACATCTCT CAAGGGTGAT ACCAGGTCCC TGTGTTCAGA GGACCATGAT 
GGTTACTATA CTACCACTCA CGAGGCTGGG AACCTGTACA CTCTCAGTGA TGGCTTGGGG 
AACCCCAGGC ACAGCATGGT CAATGTTTTT GATGGAAGGG CTCAGAGAAG CCAAGGGGAT 
CAGGCCGCTC ACCAGGATAA AATCCTTTCG AGAAACATCT CTCTGAAGAA AGCAAAGAAA 
CCACCCCTGC CACCATCCCG GACCGATTCT CTGCGAAGGA TTCCCAAAAA GAACAACCAG 
ACAAACGGTC AGGTGCTCAA TGAGAGCCTG ATCGCCTCGC TCCAGCATTC ACTGCAGCTA 
AGCCTCCCTG GCAAGGGCGG CAGCTCCCCT TCCCAGAGCC CCTGCAGTGA CTTTGAGGAA 
CCCTGGCTCC CCAGGTCCAG AAGCCAGAGC ATTGTTAGCG AGGGTAGTAG CTTGACCTCC 
ACCACCACTC CCAACGTGTA TTCTCTGTGT GGGGTCACTC CGTCCCAGAG CGACACAAGT 
AGCGTCAAGT CGGAATACAC GGACCCTTGG GGTTACTACA TTGACTACAC GAGCTTGCAG 
GAAGATCCAG GGAACCCCAC CGGGGGCTGC TCAGCCAACA CTGAGGCAGC AACTGGAAAC 
GGGCCAGTAC GCCACATCCA GGAGGGGTCG AGAGTCCCAG TGCCCCAAGT GCCGGGCTGC 
TCAGTTAGAC CAAAGATCGC CTCACCAGAG AAGTCACAGA GAGTCACCTC TCCATCCAGT 
GGGTATTCTA GCCAGTCGAA TACACCCACA GCACTCACCC CTGTGCCTGT GTTTTTAAAA 
TCAATGTCAC CAGCAAATGG AAAGGGGAAG GCCAAGCCCA AGGTACCAGA AAGAAAATCG 
TCTCTGATCT CTTCTATGTC CATCTCATCA TCGTCCACTT CTCTCTCTTC TAATACTTCT 
ACCGAAGGAA GTGGGACCAT GAAGAAACTG GATACAACCC TGGCCTCGGC CCTTGCCCCT 
CCCCCTCCTC CTCTTCCCCC TCTGCCTTCT CCGTGTCTGG CAGACAAGTC CCCTTTTCTT 
CCACCACCTC CTCCTCTAGC AGATTGCTCC GAGGGCTCTC CTCTGCCGCC CTCTCCCATG 
TTCCCCCCTC CACCACCAGA AGCCCTTGTT CCCTTCTGCT CCCCCACCGA CGGGTGCCTT 
TCTCCTTCTC CCACAGCAGT TAGCCCCTCT CTTCCAAGAT CCTTGCCTCC TGTGCCAGCA 
CCTCCACCTT TCTTGCCCTC ATCAGAACCC CCGCCCGCTC CGCCTCTGGA CCCCAAACTC 
ATGAAAGAAA ACAGGCCCTT TTTCAAAAAC TCTAGCCAGT CCGAATCCTC TAGGGAGGCC 
CTCAGGCGGC CTGCCAACAA GGAGGAGGGC TGCCGACCCC CCATGCCCCT GATCACCACA 
GAAGCTTTGC AGATGGTGCA GTTGAGGCCA GTGAGGAAGA ACTCAGGTGC CGGGGCAGTC 
CTGTTTTCTG AACCATCAGC TCAGGAACAA CGAACTCCGA CTGCTCCACA GTATCACTTA 
AAGCCATCTG CTTTCCTCAA ATCCCGAAAT AGCATAAATG AAATGGAGAG TGAAAGCCAG 
GCTGCCTCTG TGACGAGCTC GCTTCCGATG CCTGCCAAGA GCCAGAGTCA GGGTGACCAT 
GACAGTGCAG TAGAGCGTGG CGGCCTTCCG AGCTGCAGCG ATGGAGCTCC AGGCCCGGGT 
CCCAGCCTCA GGACCACTCT GCTCCCAGAC TCTTCACCCA GCAGGAAGCC ACCCCCCATC 
TCTAAGAAGC CCAAACTGTT CCTGGTGGTA CCACCTCCGC AGAGAGATTT CACAGCGGAG 
CCCACAGAGA ACGGGAGCGA AGCTTTCCCA GGCGTGCCCA GCCCTACCAG GGCAGAGGGG 
GAAGCTGTGC GGAGCCAAGA GGAGAAATCC AGCCCAGCAT CCCGAGCTGG CTCTCATGCT 
ACGGCCCCCA CCCCCGGCAG TCCGGCTCTA GAACCGGGAA CTGCAGGGTC CTTATCCTCT 
AGCATTGTGG AAGCCAATGT CCCCATGGTC CAACCTAATA CATCACCGGG CCCCACACAG 
GAAGAGTCAG GCGAGAACAG TGTGGATGGT GAGAGAAATG CGAAGAGCTG CCTGTCTCAA 
CAGGGCAGAG AAGCTGGCTT GCTGGAACCC AACACAGCTG CTTCCTCTTC AGACCCCGTG 
GATGTATCTA AGGAAGAGGG AAGCGATGAG GTGCTGACTC CGACTAAACC CAGGACAACA 
GAAGACCTGT TTGCAGCCAT TCACAGATCT AAAAGGAAAG TCCTTGGCCG GAAAGACTCA 
GAAGACGATC ACACTCGAAA TCACTCTCCC TCCCCGCCTG TGACACCCAC GAGTGCTGCC 
CCTAACCTGG CCTCTCCAAA GCAAGTAGGG TCTATCCAGA GAAGCATAAA AAAGAGCACT 
ACCAGCAGTG ACAACTTCAA AGCTCTGCTG CTAAAGAAGG GGAGTCGCTC GGACACCAGC 
GCTCGCATGT CAGCGGCTGA GATGCTCAAG TCTACGGACC CTCGCTTCCA GAGATCTAGG 
TCGGAGCCTT CGGCAGACAG CCCGGACAGC CCATCCAGCT GCTCCCCCAA CAAGAACAGG 
AGAGCTCAGG AAGAGTGGGC CAAGAACGAA GGCCTGATGC CTCGGAGTCT GTCCTTTTCG 
GGCCCTCGGT ACAGCCGCAG CAGGACGCCA CCCTCTGCGG CCAGCAGCAG GTACAGCATG 
CGGAACCGCA TCCAGAGCAG CCCCATGACC GTCATCTCCG AGGGAGAGGG AGAACCTGCT 
GAGCCAGCGG ACAACAAAGC ACGCAGGGCC CTGGATGCTA CCAGGGTGTG CTCTCTGGAC 
AGACTGACGG GTCAGGAGAT GGACCAGGCC AGCCTCCTCT GTAGCGAGGA GCCTGCCTCT 
GTGGATGGGA TAGGAAGGGC TGAGGGCAAC GGTCCCTCGG AGCAGTGTGG GGGTACGGAA 
CAGAAGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157064.1
CDS	128..4879
Translation	MVVFINAKIKSFFKLFKKKTVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	NM_001163592.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163592.1

ORF Insert Sequence:

ATGGTGGTCT TCATTAATGC AAAGATTAAA TCTTTCTTTA AACTTTTTAA AAAGAAAACG 
GTTTCCAACT TGGACGAGGA GAGTCGATGG ACTGTCCACT ACACGGCACC ATGGCATCAG 
CAAGAGAATG TGTTCCTTCC CGCCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCCAAGCTCA ACCTCAAATC AGTGCTGAGG GAATGTGACA AGCTGAGGCA AGATGGCTGC 
CGAAGTTCTC AGTACTATTC TCAGGGACCC ACCTTTGCAG CCGGCTCCAG CCCCTGCGAC 
GATTATCAAG ATGAAGACAC AGAAGCAGAC CGAAAGTGCT CTCTCTCTTC ATCTGAAGAA 
GAAAGATTTA TCGGAATCAG GAGACCTAAA ACGCCAACCT CAGGTGACTT CTCCGACCTT 
CATACTCAGA CAAACTGGAC CAAGTCCCTG CCGCTGCCAA CACCAGAAGA GAAGACGCGG 
CAGCAAGCCC AGACGGTCCA GGCTGACGTG GTTCCTATAA ACATAACTGG GGAGAATTTC 
GATCGTCAGG CCAGCCTTCG GCGGTCTCTA ATTTACACAG ACACTCTGGT AAGACGACCG 
AAGAAAGTCA AAAGGAGAAA GACAATTTCA GGAATCCCTG ACATCATTCA GAAGGAGCTA 
GCATCAGCCA CTGGCCAAGA TGATGATGGT AGTGCCCATT CGCTGTACGT CCCAGACCAC 
TACTCTACGT TAGGAAGGCT TGATAGTTAT CGGTCCACCG GGCAATGCTT GGAAACCAGG 
GACACCAGCT GTCAGACCGA AGACGTGAAG GTCATCCCAC CATCAATGAG AAGGATCAGG 
GCTCACAAGG GGGTAGGCGT TGCTGCCCAG ATGAGCCACC TCTCAGGTTC CTCAGGCAAC 
ATGTCTGTGC TGAGTGACTC AGCCGGTGTT GTGTTCCCAT CTCGCCTCAG TAACGATACT 
GGTTTCCACA GTCTTCCACG TACCGGCCCG AGAGCAAGCA CCTATTCACT GGAGGGAAGG 
ATGGGTGCCC TGGGCTCTAC TGAAGATACG GATGACACTT CTCCCTACCA GGGGGGTAGC 
TTACAGGGGC ATGAAAACTT TGCGCATTTA GGAGGTGCGT CGAGTACTGG GATGCTTTCG 
AGGCCCAAAT CCCAGCAGTT GAGGTTTTTG GAGAGTCCAG CATGTGTGGT TTCACCTCAT 
GCAGCCTACT CTACCAGTGT CATCCCAAAT GCCACGCTAC TGTCCTCTTC AGAGGTTATT 
GTCATTCACA CTGCTCAGAG TGCAGGACAG CTGGACAGTA GAACACCCGG TTCATCTTCA 
TATTCAAAAA TAAAACCCAG AGACCGTCCC ACACCCAGAT GTTCTGTGAA AGATGACCAT 
CAGTCCCCCC GTCATCACTG GAACGAGGGT CATCTCATTC ATTCACGGGC TTTAGCCTCC 
TCTGTCCCTG GTGCCACCAC GCTGTTATCC CTCCATGATT CAGAGGTCTC TCTAAATGCC 
CCAGCAAACA GGGAAAATGG ATCCCAAGCC ATACTCTATC ATTGTAGAAA CAACCCAAGC 
TTTCCTGACC ACCCTTCAGA TGTGGATGGC AAGAGCGAGT GTAGTTATTC GGGGGACAGG 
GGATGTGGCA GTTCTGAGCC CTGGGAATAT AAAACCTCCA GCAATGGGCG GGCATCCCCA 
CTGAAGCCAC ACCTGGCCAC TCCTGGTTGC TCTACTCCCA CAAGTAACGT GAGCAGCTGC 
AGTTTGGACC AAACATCTCT CAAGGGTGAT ACCAGGTCCC TGTGTTCAGA GGACCATGAT 
GGTTACTATA CTACCACTCA CGAGGCTGGG AACCTGTACA CTCTCAGTGA TGGCTTGGGG 
AACCCCAGGC ACAGCATGGT CAATGTTTTT GATGGAAGGG CTCAGAGAAG CCAAGGGGAT 
CAGGCCGCTC ACCAGGATAA AATCCTTTCG AGAAACATCT CTCTGAAGAA AGCAAAGAAA 
CCACCCCTGC CACCATCCCG GACCGATTCT CTGCGAAGGA TTCCCAAAAA GAACAACCAG 
ACAAACGGTC AGGTGCTCAA TGAGAGCCTG ATCGCCTCGC TCCAGCATTC ACTGCAGCTA 
AGCCTCCCTG GCAAGGGCGG CAGCTCCCCT TCCCAGAGCC CCTGCAGTGA CTTTGAGGAA 
CCCTGGCTCC CCAGGTCCAG AAGCCAGAGC ATTGTTAGCG AGGGTAGTAG CTTGACCTCC 
ACCACCACTC CCAACGTGTA TTCTCTGTGT GGGGTCACTC CGTCCCAGAG CGACACAAGT 
AGCGTCAAGT CGGAATACAC GGACCCTTGG GGTTACTACA TTGACTACAC GAGCTTGCAG 
GAAGATCCAG GGAACCCCAC CGGGGGCTGC TCAGCCAACA CTGAGGCAGC AACTGGAAAC 
GGGCCAGTAC GCCACATCCA GGAGGGGTCG AGAGTCCCAG TGCCCCAAGT GCCGGGCTGC 
TCAGTTAGAC CAAAGATCGC CTCACCAGAG AAGTCACAGA GAGTCACCTC TCCATCCAGT 
GGGTATTCTA GCCAGTCGAA TACACCCACA GCACTCACCC CTGTGCCTGT GTTTTTAAAA 
TCAATGTCAC CAGCAAATGG AAAGGGGAAG GCCAAGCCCA AGGTACCAGA AAGAAAATCG 
TCTCTGATCT CTTCTATGTC CATCTCATCA TCGTCCACTT CTCTCTCTTC TAATACTTCT 
ACCGAAGGAA GTGGGACCAT GAAGAAACTG GATACAACCC TGGCCTCGGC CCTTGCCCCT 
CCCCCTCCTC CTCTTCCCCC TCTGCCTTCT CCGTGTCTGG CAGACAAGTC CCCTTTTCTT 
CCACCACCTC CTCCTCTAGC AGATTGCTCC GAGGGCTCTC CTCTGCCGCC CTCTCCCATG 
TTCCCCCCTC CACCACCAGA AGCCCTTGTT CCCTTCTGCT CCCCCACCGA CGGGTGCCTT 
TCTCCTTCTC CCACAGCAGT TAGCCCCTCT CTTCCAAGAT CCTTGCCTCC TGTGCCAGCA 
CCTCCACCTT TCTTGCCCTC ATCAGAACCC CCGCCCGCTC CGCCTCTGGA CCCCAAACTC 
ATGAAAGAAA ACAGGCCCTT TTTCAAAAAC TCTAGCCAGT CCGAATCCTC TAGGGAGGCC 
CTCAGGCGGC CTGCCAACAA GGAGGAGGGC TGCCGACCCC CCATGCCCCT GATCACCACA 
GAAGCTTTGC AGATGGTGCA GTTGAGGCCA GTGAGGAAGA ACTCAGGTGC CGGGGCAGTC 
CTGTTTTCTG AACCATCAGC TCAGGAACAA CGAACTCCGA CTGCTCCACA GTATCACTTA 
AAGCCATCTG CTTTCCTCAA ATCCCGAAAT AGCATAAATG AAATGGAGAG TGAAAGCCAG 
GCTGCCTCTG TGACGAGCTC GCTTCCGATG CCTGCCAAGA GCCAGAGTCA GGGTGACCAT 
GACAGTGCAG TAGAGCGTGG CGGCCTTCCG AGCTGCAGCG ATGGAGCTCC AGGCCCGGGT 
CCCAGCCTCA GGACCACTCT GCTCCCAGAC TCTTCACCCA GCAGGAAGCC ACCCCCCATC 
TCTAAGAAGC CCAAACTGTT CCTGGTGGTA CCACCTCCGC AGAGAGATTT CACAGCGGAG 
CCCACAGAGA ACGGGAGCGA AGCTTTCCCA GGCGTGCCCA GCCCTACCAG GGCAGAGGGG 
GAAGCTGTGC GGAGCCAAGA GGAGAAATCC AGCCCAGCAT CCCGAGCTGG CTCTCATGCT 
ACGGCCCCCA CCCCCGGCAG TCCGGCTCTA GAACCGGGAA CTGCAGGGTC CTTATCCTCT 
AGCATTGTGG AAGCCAATGT CCCCATGGTC CAACCTAATA CATCACCGGG CCCCACACAG 
GAAGAGTCAG GCGAGAACAG TGTGGATGGT GAGAGAAATG CGAAGAGCTG CCTGTCTCAA 
CAGGGCAGAG AAGCTGGCTT GCTGGAACCC AACACAGCTG CTTCCTCTTC AGACCCCGTG 
GATGTATCTA AGGAAGAGGG AAGCGATGAG GTGCTGACTC CGACTAAACC CAGGACAACA 
GAAGACCTGT TTGCAGCCAT TCACAGATCT AAAAGGAAAG TCCTTGGCCG GAAAGACTCA 
GAAGACGATC ACACTCGAAA TCACTCTCCC TCCCCGCCTG TGACACCCAC GAGTGCTGCC 
CCTAACCTGG CCTCTCCAAA GCAAGTAGGG TCTATCCAGA GAAGCATAAA AAAGAGCACT 
ACCAGCAGTG ACAACTTCAA AGCTCTGCTG CTAAAGAAGG GGAGTCGCTC GGACACCAGC 
GCTCGCATGT CAGCGGCTGA GATGCTCAAG TCTACGGACC CTCGCTTCCA GAGATCTAGG 
TCGGAGCCTT CGGCAGACAG CCCGGACAGC CCATCCAGCT GCTCCCCCAA CAAGAACAGG 
AGAGCTCAGG AAGAGTGGGC CAAGAACGAA GGCCTGATGC CTCGGAGTCT GTCCTTTTCG 
GGCCCTCGGT ACAGCCGCAG CAGGACGCCA CCCTCTGCGG CCAGCAGCAG GTACAGCATG 
CGGAACCGCA TCCAGAGCAG CCCCATGACC GTCATCTCCG AGGGAGAGGG AGAACCTGCT 
GAGCCAGCGG ACAACAAAGC ACGCAGGGCC CTGGATGCTA CCAGGGTGTG CTCTCTGGAC 
AGACTGACGG GTCAGGAGAT GGACCAGGCC AGCCTCCTCT GTAGCGAGGA GCCTGCCTCT 
GTGGATGGGA TAGGAAGGGC TGAGGGCAAC GGTCCCTCGG AGCAGTGTGG GGGTACGGAA 
CAGAAGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006512686.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512749.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.

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CloneID	OMu43843
Clone ID Related Accession (Same CDS sequence)	XM_006512686.3 , XM_006512686.4
Accession Version	XM_006512686.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512686.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG 
AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT 
CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA 
GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGGGGAGAA TTTCGATCGT 
CAGGCCAGCC TTCGGCGGTC TCTAATTTAC ACAGACACTC TGGTAAGACG ACCGAAGAAA 
GTCAAAAGGA GAAAGACAAT TTCAGGAATC CCTGACATCA TTCAGAAGGA GCTAGCATCA 
GCCACTGGCC AAGATGATGA TGGTAGTGCC CATTCGCTGT ACGTCCCAGA CCACTACTCT 
ACGTTAGGAA GGCTTGATAG TTATCGGTCC ACCGGGCAAT GCTTGGAAAC CAGGGACACC 
AGCTGTCAGA CCGAAGACGT GAAGGTCATC CCACCATCAA TGAGAAGGAT CAGGGCTCAC 
AAGGGGGTAG GCGTTGCTGC CCAGATGAGC CACCTCTCAG GTTCCTCAGG CAACATGTCT 
GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC CCATCTCGCC TCAGTAACGA TACTGGTTTC 
CACAGTCTTC CACGTACCGG CCCGAGAGCA AGCACCTATT CACTGGAGGG AAGGATGGGT 
GCCCTGGGCT CTACTGAAGA TACGGATGAC ACTTCTCCCT ACCAGGGGGG TAGCTTACAG 
GGGCATGAAA ACTTTGCGCA TTTAGGAGGT GCGTCGAGTA CTGGGATGCT TTCGAGGCCC 
AAATCCCAGC AGTTGAGGTT TTTGGAGAGT CCAGCATGTG TGGTTTCACC TCATGCAGCC 
TACTCTACCA GTGTCATCCC AAATGCCACG CTACTGTCCT CTTCAGAGGT TATTGTCATT 
CACACTGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAACAC CCGGTTCATC TTCATATTCA 
AAAATAAAAC CCAGAGACCG TCCCACACCC AGATGTTCTG TGAAAGATGA CCATCAGTCC 
CCCCGTCATC ACTGGAACGA GGGTCATCTC ATTCATTCAC GGGCTTTAGC CTCCTCTGTC 
CCTGGTGCCA CCACGCTGTT ATCCCTCCAT GATTCAGAGG TCTCTCTAAA TGCCCCAGCA 
AACAGGGAAA ATGGATCCCA AGCCATACTC TATCATTGTA GAAACAACCC AAGCTTTCCT 
GACCACCCTT CAGATGTGGA TGGCAAGAGC GAGTGTAGTT ATTCGGGGGA CAGGGGATGT 
GGCAGTTCTG AGCCCTGGGA ATATAAAACC TCCAGCAATG GGCGGGCATC CCCACTGAAG 
CCACACCTGG CCACTCCTGG TTGCTCTACT CCCACAAGTA ACGTGAGCAG CTGCAGTTTG 
GACCAAACAT CTCTCAAGGG TGATACCAGG TCCCTGTGTT CAGAGGACCA TGATGGTTAC 
TATACTACCA CTCACGAGGC TGGGAACCTG TACACTCTCA GTGATGGCTT GGGGAACCCC 
AGGCACAGCA TGGTCAATGT TTTTGATGGA AGGGCTCAGA GAAGCCAAGG GGATCAGGCC 
GCTCACCAGG ATAAAATCCT TTCGAGAAAC ATCTCTCTGA AGAAAGCAAA GAAACCACCC 
CTGCCACCAT CCCGGACCGA TTCTCTGCGA AGGATTCCCA AAAAGAACAA CCAGACAAAC 
GGTCAGGTGC TCAATGAGAG CCTGATCGCC TCGCTCCAGC ATTCACTGCA GCTAAGCCTC 
CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG AGCCCCTGCA GTGACTTTGA GGAACCCTGG 
CTCCCCAGGT CCAGAAGCCA GAGCATTGTT AGCGAGGGTA GTAGCTTGAC CTCCACCACC 
ACTCCCAACG TGTATTCTCT GTGTGGGGTC ACTCCGTCCC AGAGCGACAC AAGTAGCGTC 
AAGTCGGAAT ACACGGACCC TTGGGGTTAC TACATTGACT ACACGAGCTT GCAGGAAGAT 
CCAGGGAACC CCACCGGGGG CTGCTCAGCC AACACTGAGG CAGCAACTGG AAACGGGCCA 
GTACGCCACA TCCAGGAGGG GTCGAGAGTC CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT 
AGACCAAAGA TCGCCTCACC AGAGAAGTCA CAGAGAGTCA CCTCTCCATC CAGTGGGTAT 
TCTAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAATG 
TCACCAGCAA ATGGAAAGGG GAAGGCCAAG CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG 
ATCTCTTCTA TGTCCATCTC ATCATCGTCC ACTTCTCTCT CTTCTAATAC TTCTACCGAA 
GGAAGTGGGA CCATGAAGAA ACTGGATACA ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT 
CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT CTGGCAGACA AGTCCCCTTT TCTTCCACCA 
CCTCCTCCTC TAGCAGATTG CTCCGAGGGC TCTCCTCTGC CGCCCTCTCC CATGTTCCCC 
CCTCCACCAC CAGAAGCCCT TGTTCCCTTC TGCTCCCCCA CCGACGGGTG CCTTTCTCCT 
TCTCCCACAG CAGTTAGCCC CTCTCTTCCA AGATCCTTGC CTCCTGTGCC AGCACCTCCA 
CCTTTCTTGC CCTCATCAGA ACCCCCGCCC GCTCCGCCTC TGGACCCCAA ACTCATGAAA 
GAAAACAGGC CCTTTTTCAA AAACTCTAGC CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG 
CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA CCCCCCATGC CCCTGATCAC CACAGAAGCT 
TTGCAGATGG TGCAGTTGAG GCCAGTGAGG AAGAACTCAG GTGCCGGGGC AGTCCTGTTT 
TCTGAACCAT CAGCTCAGGA ACAACGAACT CCGACTGCTC CACAGTATCA CTTAAAGCCA 
TCTGCTTTCC TCAAATCCCG AAATAGCATA AATGAAATGG AGAGTGAAAG CCAGGCTGCC 
TCTGTGACGA GCTCGCTTCC GATGCCTGCC AAGAGCCAGA GTCAGGGTGA CCATGACAGT 
GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC AGCGATGGAG CTCCAGGCCC GGGTCCCAGC 
CTCAGGACCA CTCTGCTCCC AGACTCTTCA CCCAGCAGGA AGCCACCCCC CATCTCTAAG 
AAGCCCAAAC TGTTCCTGGT GGTACCACCT CCGCAGAGAG ATTTCACAGC GGAGCCCACA 
GAGAACGGGA GCGAAGCTTT CCCAGGCGTG CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT 
GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA GCATCCCGAG CTGGCTCTCA TGCTACGGCC 
CCCACCCCCG GCAGTCCGGC TCTAGAACCG GGAACTGCAG GGTCCTTATC CTCTAGCATT 
GTGGAAGCCA ATGTCCCCAT GGTCCAACCT AATACATCAC CGGGCCCCAC ACAGGAAGAG 
TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA AATGCGAAGA GCTGCCTGTC TCAACAGGGC 
AGAGAAGCTG GCTTGCTGGA ACCCAACACA GCTGCTTCCT CTTCAGACCC CGTGGATGTA 
TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG ACTCCGACTA AACCCAGGAC AACAGAAGAC 
CTGTTTGCAG CCATTCACAG ATCTAAAAGG AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC 
GATCACACTC GAAATCACTC TCCCTCCCCG CCTGTGACAC CCACGAGTGC TGCCCCTAAC 
CTGGCCTCTC CAAAGCAAGT AGGGTCTATC CAGAGAAGCA TAAAAAAGAG CACTACCAGC 
AGTGACAACT TCAAAGCTCT GCTGCTAAAG AAGGGGAGTC GCTCGGACAC CAGCGCTCGC 
ATGTCAGCGG CTGAGATGCT CAAGTCTACG GACCCTCGCT TCCAGAGATC TAGGTCGGAG 
CCTTCGGCAG ACAGCCCGGA CAGCCCATCC AGCTGCTCCC CCAACAAGAA CAGGAGAGCT 
CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT 
CGGTACAGCC GCAGCAGGAC GCCACCCTCT GCGGCCAGCA GCAGGTACAG CATGCGGAAC 
CGCATCCAGA GCAGCCCCAT GACCGTCATC TCCGAGGGAG AGGGAGAACC TGCTGAGCCA 
GCGGACAACA AAGCACGCAG GGCCCTGGAT GCTACCAGGG TGTGCTCTCT GGACAGACTG 
ACGGGTCAGG AGATGGACCA GGCCAGCCTC CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT 
GGGATAGGAA GGGCTGAGGG CAACGGTCCC TCGGAGCAGT GTGGGGGTAC GGAACAGAAG 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512749.1
CDS	424..5409
Translation	MPFHQRSVEPARLRRPDEAALARAGDPPLFRSLEQVSSHTLVCLLAQLADLSRCAGDIFGELEGQAAALGHRTAELHRRLDALHAAAARLDHRRVKIPVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_006512686.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512686.3

ORF Insert Sequence:

ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG 
AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT 
CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA 
GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGGGGAGAA TTTCGATCGT 
CAGGCCAGCC TTCGGCGGTC TCTAATTTAC ACAGACACTC TGGTAAGACG ACCGAAGAAA 
GTCAAAAGGA GAAAGACAAT TTCAGGAATC CCTGACATCA TTCAGAAGGA GCTAGCATCA 
GCCACTGGCC AAGATGATGA TGGTAGTGCC CATTCGCTGT ACGTCCCAGA CCACTACTCT 
ACGTTAGGAA GGCTTGATAG TTATCGGTCC ACCGGGCAAT GCTTGGAAAC CAGGGACACC 
AGCTGTCAGA CCGAAGACGT GAAGGTCATC CCACCATCAA TGAGAAGGAT CAGGGCTCAC 
AAGGGGGTAG GCGTTGCTGC CCAGATGAGC CACCTCTCAG GTTCCTCAGG CAACATGTCT 
GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC CCATCTCGCC TCAGTAACGA TACTGGTTTC 
CACAGTCTTC CACGTACCGG CCCGAGAGCA AGCACCTATT CACTGGAGGG AAGGATGGGT 
GCCCTGGGCT CTACTGAAGA TACGGATGAC ACTTCTCCCT ACCAGGGGGG TAGCTTACAG 
GGGCATGAAA ACTTTGCGCA TTTAGGAGGT GCGTCGAGTA CTGGGATGCT TTCGAGGCCC 
AAATCCCAGC AGTTGAGGTT TTTGGAGAGT CCAGCATGTG TGGTTTCACC TCATGCAGCC 
TACTCTACCA GTGTCATCCC AAATGCCACG CTACTGTCCT CTTCAGAGGT TATTGTCATT 
CACACTGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAACAC CCGGTTCATC TTCATATTCA 
AAAATAAAAC CCAGAGACCG TCCCACACCC AGATGTTCTG TGAAAGATGA CCATCAGTCC 
CCCCGTCATC ACTGGAACGA GGGTCATCTC ATTCATTCAC GGGCTTTAGC CTCCTCTGTC 
CCTGGTGCCA CCACGCTGTT ATCCCTCCAT GATTCAGAGG TCTCTCTAAA TGCCCCAGCA 
AACAGGGAAA ATGGATCCCA AGCCATACTC TATCATTGTA GAAACAACCC AAGCTTTCCT 
GACCACCCTT CAGATGTGGA TGGCAAGAGC GAGTGTAGTT ATTCGGGGGA CAGGGGATGT 
GGCAGTTCTG AGCCCTGGGA ATATAAAACC TCCAGCAATG GGCGGGCATC CCCACTGAAG 
CCACACCTGG CCACTCCTGG TTGCTCTACT CCCACAAGTA ACGTGAGCAG CTGCAGTTTG 
GACCAAACAT CTCTCAAGGG TGATACCAGG TCCCTGTGTT CAGAGGACCA TGATGGTTAC 
TATACTACCA CTCACGAGGC TGGGAACCTG TACACTCTCA GTGATGGCTT GGGGAACCCC 
AGGCACAGCA TGGTCAATGT TTTTGATGGA AGGGCTCAGA GAAGCCAAGG GGATCAGGCC 
GCTCACCAGG ATAAAATCCT TTCGAGAAAC ATCTCTCTGA AGAAAGCAAA GAAACCACCC 
CTGCCACCAT CCCGGACCGA TTCTCTGCGA AGGATTCCCA AAAAGAACAA CCAGACAAAC 
GGTCAGGTGC TCAATGAGAG CCTGATCGCC TCGCTCCAGC ATTCACTGCA GCTAAGCCTC 
CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG AGCCCCTGCA GTGACTTTGA GGAACCCTGG 
CTCCCCAGGT CCAGAAGCCA GAGCATTGTT AGCGAGGGTA GTAGCTTGAC CTCCACCACC 
ACTCCCAACG TGTATTCTCT GTGTGGGGTC ACTCCGTCCC AGAGCGACAC AAGTAGCGTC 
AAGTCGGAAT ACACGGACCC TTGGGGTTAC TACATTGACT ACACGAGCTT GCAGGAAGAT 
CCAGGGAACC CCACCGGGGG CTGCTCAGCC AACACTGAGG CAGCAACTGG AAACGGGCCA 
GTACGCCACA TCCAGGAGGG GTCGAGAGTC CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT 
AGACCAAAGA TCGCCTCACC AGAGAAGTCA CAGAGAGTCA CCTCTCCATC CAGTGGGTAT 
TCTAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAATG 
TCACCAGCAA ATGGAAAGGG GAAGGCCAAG CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG 
ATCTCTTCTA TGTCCATCTC ATCATCGTCC ACTTCTCTCT CTTCTAATAC TTCTACCGAA 
GGAAGTGGGA CCATGAAGAA ACTGGATACA ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT 
CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT CTGGCAGACA AGTCCCCTTT TCTTCCACCA 
CCTCCTCCTC TAGCAGATTG CTCCGAGGGC TCTCCTCTGC CGCCCTCTCC CATGTTCCCC 
CCTCCACCAC CAGAAGCCCT TGTTCCCTTC TGCTCCCCCA CCGACGGGTG CCTTTCTCCT 
TCTCCCACAG CAGTTAGCCC CTCTCTTCCA AGATCCTTGC CTCCTGTGCC AGCACCTCCA 
CCTTTCTTGC CCTCATCAGA ACCCCCGCCC GCTCCGCCTC TGGACCCCAA ACTCATGAAA 
GAAAACAGGC CCTTTTTCAA AAACTCTAGC CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG 
CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA CCCCCCATGC CCCTGATCAC CACAGAAGCT 
TTGCAGATGG TGCAGTTGAG GCCAGTGAGG AAGAACTCAG GTGCCGGGGC AGTCCTGTTT 
TCTGAACCAT CAGCTCAGGA ACAACGAACT CCGACTGCTC CACAGTATCA CTTAAAGCCA 
TCTGCTTTCC TCAAATCCCG AAATAGCATA AATGAAATGG AGAGTGAAAG CCAGGCTGCC 
TCTGTGACGA GCTCGCTTCC GATGCCTGCC AAGAGCCAGA GTCAGGGTGA CCATGACAGT 
GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC AGCGATGGAG CTCCAGGCCC GGGTCCCAGC 
CTCAGGACCA CTCTGCTCCC AGACTCTTCA CCCAGCAGGA AGCCACCCCC CATCTCTAAG 
AAGCCCAAAC TGTTCCTGGT GGTACCACCT CCGCAGAGAG ATTTCACAGC GGAGCCCACA 
GAGAACGGGA GCGAAGCTTT CCCAGGCGTG CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT 
GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA GCATCCCGAG CTGGCTCTCA TGCTACGGCC 
CCCACCCCCG GCAGTCCGGC TCTAGAACCG GGAACTGCAG GGTCCTTATC CTCTAGCATT 
GTGGAAGCCA ATGTCCCCAT GGTCCAACCT AATACATCAC CGGGCCCCAC ACAGGAAGAG 
TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA AATGCGAAGA GCTGCCTGTC TCAACAGGGC 
AGAGAAGCTG GCTTGCTGGA ACCCAACACA GCTGCTTCCT CTTCAGACCC CGTGGATGTA 
TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG ACTCCGACTA AACCCAGGAC AACAGAAGAC 
CTGTTTGCAG CCATTCACAG ATCTAAAAGG AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC 
GATCACACTC GAAATCACTC TCCCTCCCCG CCTGTGACAC CCACGAGTGC TGCCCCTAAC 
CTGGCCTCTC CAAAGCAAGT AGGGTCTATC CAGAGAAGCA TAAAAAAGAG CACTACCAGC 
AGTGACAACT TCAAAGCTCT GCTGCTAAAG AAGGGGAGTC GCTCGGACAC CAGCGCTCGC 
ATGTCAGCGG CTGAGATGCT CAAGTCTACG GACCCTCGCT TCCAGAGATC TAGGTCGGAG 
CCTTCGGCAG ACAGCCCGGA CAGCCCATCC AGCTGCTCCC CCAACAAGAA CAGGAGAGCT 
CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT 
CGGTACAGCC GCAGCAGGAC GCCACCCTCT GCGGCCAGCA GCAGGTACAG CATGCGGAAC 
CGCATCCAGA GCAGCCCCAT GACCGTCATC TCCGAGGGAG AGGGAGAACC TGCTGAGCCA 
GCGGACAACA AAGCACGCAG GGCCCTGGAT GCTACCAGGG TGTGCTCTCT GGACAGACTG 
ACGGGTCAGG AGATGGACCA GGCCAGCCTC CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT 
GGGATAGGAA GGGCTGAGGG CAACGGTCCC TCGGAGCAGT GTGGGGGTAC GGAACAGAAG 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006512687.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512750.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.

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CloneID	OMu43844
Clone ID Related Accession (Same CDS sequence)	XM_006512687.3 , XM_006512687.4
Accession Version	XM_006512687.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG ACGGCTCCTC AGGTTCCTTC GGAATCAAGG CAAGCCCAGG CTCCCTCTCA 
CGTGCTGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTCCGC 
AGTGATGGAG AGCTGTCCGT TTGTGGGCAC CAGGTGGAAG CCGATGATGA GAACTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTGGACG AGGAGAGTCG ATGGACTGTC 
CACTACACGG CACCATGGCA TCAGCAAGAG AATGTGTTCC TTCCCGCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCAAGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCGGCT CCAGCCCCTG CGACGATTAT CAAGATGAAG ACACAGAAGC AGACCGAAAG 
TGCTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATCGGAA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCCGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC GCGGCAGCAA GCCCAGACGG TCCAGGCTGA CGTGGTTCCT 
ATAAACATAA CTGGGGAGAA TTTCGATCGT CAGGCCAGCC TTCGGCGGTC TCTAATTTAC 
ACAGACACTC TGGTAAGACG ACCGAAGAAA GTCAAAAGGA GAAAGACAAT TTCAGGAATC 
CCTGACATCA TTCAGAAGGA GCTAGCATCA GCCACTGGCC AAGATGATGA TGGTAGTGCC 
CATTCGCTGT ACGTCCCAGA CCACTACTCT ACGTTAGGAA GGCTTGATAG TTATCGGTCC 
ACCGGGCAAT GCTTGGAAAC CAGGGACACC AGCTGTCAGA CCGAAGACGT GAAGGTCATC 
CCACCATCAA TGAGAAGGAT CAGGGCTCAC AAGGGGGTAG GCGTTGCTGC CCAGATGAGC 
CACCTCTCAG GTTCCTCAGG CAACATGTCT GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC 
CCATCTCGCC TCAGTAACGA TACTGGTTTC CACAGTCTTC CACGTACCGG CCCGAGAGCA 
AGCACCTATT CACTGGAGGG AAGGATGGGT GCCCTGGGCT CTACTGAAGA TACGGATGAC 
ACTTCTCCCT ACCAGGGGGG TAGCTTACAG GGGCATGAAA ACTTTGCGCA TTTAGGAGGT 
GCGTCGAGTA CTGGGATGCT TTCGAGGCCC AAATCCCAGC AGTTGAGGTT TTTGGAGAGT 
CCAGCATGTG TGGTTTCACC TCATGCAGCC TACTCTACCA GTGTCATCCC AAATGCCACG 
CTACTGTCCT CTTCAGAGGT TATTGTCATT CACACTGCTC AGAGTGCAGG ACAGCTGGAC 
AGTAGAACAC CCGGTTCATC TTCATATTCA AAAATAAAAC CCAGAGACCG TCCCACACCC 
AGATGTTCTG TGAAAGATGA CCATCAGTCC CCCCGTCATC ACTGGAACGA GGGTCATCTC 
ATTCATTCAC GGGCTTTAGC CTCCTCTGTC CCTGGTGCCA CCACGCTGTT ATCCCTCCAT 
GATTCAGAGG TCTCTCTAAA TGCCCCAGCA AACAGGGAAA ATGGATCCCA AGCCATACTC 
TATCATTGTA GAAACAACCC AAGCTTTCCT GACCACCCTT CAGATGTGGA TGGCAAGAGC 
GAGTGTAGTT ATTCGGGGGA CAGGGGATGT GGCAGTTCTG AGCCCTGGGA ATATAAAACC 
TCCAGCAATG GGCGGGCATC CCCACTGAAG CCACACCTGG CCACTCCTGG TTGCTCTACT 
CCCACAAGTA ACGTGAGCAG CTGCAGTTTG GACCAAACAT CTCTCAAGGG TGATACCAGG 
TCCCTGTGTT CAGAGGACCA TGATGGTTAC TATACTACCA CTCACGAGGC TGGGAACCTG 
TACACTCTCA GTGATGGCTT GGGGAACCCC AGGCACAGCA TGGTCAATGT TTTTGATGGA 
AGGGCTCAGA GAAGCCAAGG GGATCAGGCC GCTCACCAGG ATAAAATCCT TTCGAGAAAC 
ATCTCTCTGA AGAAAGCAAA GAAACCACCC CTGCCACCAT CCCGGACCGA TTCTCTGCGA 
AGGATTCCCA AAAAGAACAA CCAGACAAAC GGTCAGGTGC TCAATGAGAG CCTGATCGCC 
TCGCTCCAGC ATTCACTGCA GCTAAGCCTC CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG 
AGCCCCTGCA GTGACTTTGA GGAACCCTGG CTCCCCAGGT CCAGAAGCCA GAGCATTGTT 
AGCGAGGGTA GTAGCTTGAC CTCCACCACC ACTCCCAACG TGTATTCTCT GTGTGGGGTC 
ACTCCGTCCC AGAGCGACAC AAGTAGCGTC AAGTCGGAAT ACACGGACCC TTGGGGTTAC 
TACATTGACT ACACGAGCTT GCAGGAAGAT CCAGGGAACC CCACCGGGGG CTGCTCAGCC 
AACACTGAGG CAGCAACTGG AAACGGGCCA GTACGCCACA TCCAGGAGGG GTCGAGAGTC 
CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT AGACCAAAGA TCGCCTCACC AGAGAAGTCA 
CAGAGAGTCA CCTCTCCATC CAGTGGGTAT TCTAGCCAGT CGAATACACC CACAGCACTC 
ACCCCTGTGC CTGTGTTTTT AAAATCAATG TCACCAGCAA ATGGAAAGGG GAAGGCCAAG 
CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG ATCTCTTCTA TGTCCATCTC ATCATCGTCC 
ACTTCTCTCT CTTCTAATAC TTCTACCGAA GGAAGTGGGA CCATGAAGAA ACTGGATACA 
ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT 
CTGGCAGACA AGTCCCCTTT TCTTCCACCA CCTCCTCCTC TAGCAGATTG CTCCGAGGGC 
TCTCCTCTGC CGCCCTCTCC CATGTTCCCC CCTCCACCAC CAGAAGCCCT TGTTCCCTTC 
TGCTCCCCCA CCGACGGGTG CCTTTCTCCT TCTCCCACAG CAGTTAGCCC CTCTCTTCCA 
AGATCCTTGC CTCCTGTGCC AGCACCTCCA CCTTTCTTGC CCTCATCAGA ACCCCCGCCC 
GCTCCGCCTC TGGACCCCAA ACTCATGAAA GAAAACAGGC CCTTTTTCAA AAACTCTAGC 
CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA 
CCCCCCATGC CCCTGATCAC CACAGAAGCT TTGCAGATGG TGCAGTTGAG GCCAGTGAGG 
AAGAACTCAG GTGCCGGGGC AGTCCTGTTT TCTGAACCAT CAGCTCAGGA ACAACGAACT 
CCGACTGCTC CACAGTATCA CTTAAAGCCA TCTGCTTTCC TCAAATCCCG AAATAGCATA 
AATGAAATGG AGAGTGAAAG CCAGGCTGCC TCTGTGACGA GCTCGCTTCC GATGCCTGCC 
AAGAGCCAGA GTCAGGGTGA CCATGACAGT GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC 
AGCGATGGAG CTCCAGGCCC GGGTCCCAGC CTCAGGACCA CTCTGCTCCC AGACTCTTCA 
CCCAGCAGGA AGCCACCCCC CATCTCTAAG AAGCCCAAAC TGTTCCTGGT GGTACCACCT 
CCGCAGAGAG ATTTCACAGC GGAGCCCACA GAGAACGGGA GCGAAGCTTT CCCAGGCGTG 
CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA 
GCATCCCGAG CTGGCTCTCA TGCTACGGCC CCCACCCCCG GCAGTCCGGC TCTAGAACCG 
GGAACTGCAG GGTCCTTATC CTCTAGCATT GTGGAAGCCA ATGTCCCCAT GGTCCAACCT 
AATACATCAC CGGGCCCCAC ACAGGAAGAG TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA 
AATGCGAAGA GCTGCCTGTC TCAACAGGGC AGAGAAGCTG GCTTGCTGGA ACCCAACACA 
GCTGCTTCCT CTTCAGACCC CGTGGATGTA TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG 
ACTCCGACTA AACCCAGGAC AACAGAAGAC CTGTTTGCAG CCATTCACAG ATCTAAAAGG 
AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC GATCACACTC GAAATCACTC TCCCTCCCCG 
CCTGTGACAC CCACGAGTGC TGCCCCTAAC CTGGCCTCTC CAAAGCAAGT AGGGTCTATC 
CAGAGAAGCA TAAAAAAGAG CACTACCAGC AGTGACAACT TCAAAGCTCT GCTGCTAAAG 
AAGGGGAGTC GCTCGGACAC CAGCGCTCGC ATGTCAGCGG CTGAGATGCT CAAGTCTACG 
GACCCTCGCT TCCAGAGATC TAGGTCGGAG CCTTCGGCAG ACAGCCCGGA CAGCCCATCC 
AGCTGCTCCC CCAACAAGAA CAGGAGAGCT CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG 
ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT CGGTACAGCC GCAGCAGGAC GCCACCCTCT 
GCGGCCAGCA GCAGGTACAG CATGCGGAAC CGCATCCAGA GCAGCCCCAT GACCGTCATC 
TCCGAGGGAG AGGGAGAACC TGCTGAGCCA GCGGACAACA AAGCACGCAG GGCCCTGGAT 
GCTACCAGGG TGTGCTCTCT GGACAGACTG ACGGGTCAGG AGATGGACCA GGCCAGCCTC 
CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT GGGATAGGAA GGGCTGAGGG CAACGGTCCC 
TCGGAGCAGT GTGGGGGTAC GGAACAGAAG AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512750.1
CDS	856..5751
Translation	MKKDGSSGSFGIKASPGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGHQVEADDENWIYRTQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_006512687.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512687.3

ORF Insert Sequence:

ATGAAAAAGG ACGGCTCCTC AGGTTCCTTC GGAATCAAGG CAAGCCCAGG CTCCCTCTCA 
CGTGCTGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTCCGC 
AGTGATGGAG AGCTGTCCGT TTGTGGGCAC CAGGTGGAAG CCGATGATGA GAACTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTGGACG AGGAGAGTCG ATGGACTGTC 
CACTACACGG CACCATGGCA TCAGCAAGAG AATGTGTTCC TTCCCGCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCAAGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCGGCT CCAGCCCCTG CGACGATTAT CAAGATGAAG ACACAGAAGC AGACCGAAAG 
TGCTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATCGGAA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCCGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC GCGGCAGCAA GCCCAGACGG TCCAGGCTGA CGTGGTTCCT 
ATAAACATAA CTGGGGAGAA TTTCGATCGT CAGGCCAGCC TTCGGCGGTC TCTAATTTAC 
ACAGACACTC TGGTAAGACG ACCGAAGAAA GTCAAAAGGA GAAAGACAAT TTCAGGAATC 
CCTGACATCA TTCAGAAGGA GCTAGCATCA GCCACTGGCC AAGATGATGA TGGTAGTGCC 
CATTCGCTGT ACGTCCCAGA CCACTACTCT ACGTTAGGAA GGCTTGATAG TTATCGGTCC 
ACCGGGCAAT GCTTGGAAAC CAGGGACACC AGCTGTCAGA CCGAAGACGT GAAGGTCATC 
CCACCATCAA TGAGAAGGAT CAGGGCTCAC AAGGGGGTAG GCGTTGCTGC CCAGATGAGC 
CACCTCTCAG GTTCCTCAGG CAACATGTCT GTGCTGAGTG ACTCAGCCGG TGTTGTGTTC 
CCATCTCGCC TCAGTAACGA TACTGGTTTC CACAGTCTTC CACGTACCGG CCCGAGAGCA 
AGCACCTATT CACTGGAGGG AAGGATGGGT GCCCTGGGCT CTACTGAAGA TACGGATGAC 
ACTTCTCCCT ACCAGGGGGG TAGCTTACAG GGGCATGAAA ACTTTGCGCA TTTAGGAGGT 
GCGTCGAGTA CTGGGATGCT TTCGAGGCCC AAATCCCAGC AGTTGAGGTT TTTGGAGAGT 
CCAGCATGTG TGGTTTCACC TCATGCAGCC TACTCTACCA GTGTCATCCC AAATGCCACG 
CTACTGTCCT CTTCAGAGGT TATTGTCATT CACACTGCTC AGAGTGCAGG ACAGCTGGAC 
AGTAGAACAC CCGGTTCATC TTCATATTCA AAAATAAAAC CCAGAGACCG TCCCACACCC 
AGATGTTCTG TGAAAGATGA CCATCAGTCC CCCCGTCATC ACTGGAACGA GGGTCATCTC 
ATTCATTCAC GGGCTTTAGC CTCCTCTGTC CCTGGTGCCA CCACGCTGTT ATCCCTCCAT 
GATTCAGAGG TCTCTCTAAA TGCCCCAGCA AACAGGGAAA ATGGATCCCA AGCCATACTC 
TATCATTGTA GAAACAACCC AAGCTTTCCT GACCACCCTT CAGATGTGGA TGGCAAGAGC 
GAGTGTAGTT ATTCGGGGGA CAGGGGATGT GGCAGTTCTG AGCCCTGGGA ATATAAAACC 
TCCAGCAATG GGCGGGCATC CCCACTGAAG CCACACCTGG CCACTCCTGG TTGCTCTACT 
CCCACAAGTA ACGTGAGCAG CTGCAGTTTG GACCAAACAT CTCTCAAGGG TGATACCAGG 
TCCCTGTGTT CAGAGGACCA TGATGGTTAC TATACTACCA CTCACGAGGC TGGGAACCTG 
TACACTCTCA GTGATGGCTT GGGGAACCCC AGGCACAGCA TGGTCAATGT TTTTGATGGA 
AGGGCTCAGA GAAGCCAAGG GGATCAGGCC GCTCACCAGG ATAAAATCCT TTCGAGAAAC 
ATCTCTCTGA AGAAAGCAAA GAAACCACCC CTGCCACCAT CCCGGACCGA TTCTCTGCGA 
AGGATTCCCA AAAAGAACAA CCAGACAAAC GGTCAGGTGC TCAATGAGAG CCTGATCGCC 
TCGCTCCAGC ATTCACTGCA GCTAAGCCTC CCTGGCAAGG GCGGCAGCTC CCCTTCCCAG 
AGCCCCTGCA GTGACTTTGA GGAACCCTGG CTCCCCAGGT CCAGAAGCCA GAGCATTGTT 
AGCGAGGGTA GTAGCTTGAC CTCCACCACC ACTCCCAACG TGTATTCTCT GTGTGGGGTC 
ACTCCGTCCC AGAGCGACAC AAGTAGCGTC AAGTCGGAAT ACACGGACCC TTGGGGTTAC 
TACATTGACT ACACGAGCTT GCAGGAAGAT CCAGGGAACC CCACCGGGGG CTGCTCAGCC 
AACACTGAGG CAGCAACTGG AAACGGGCCA GTACGCCACA TCCAGGAGGG GTCGAGAGTC 
CCAGTGCCCC AAGTGCCGGG CTGCTCAGTT AGACCAAAGA TCGCCTCACC AGAGAAGTCA 
CAGAGAGTCA CCTCTCCATC CAGTGGGTAT TCTAGCCAGT CGAATACACC CACAGCACTC 
ACCCCTGTGC CTGTGTTTTT AAAATCAATG TCACCAGCAA ATGGAAAGGG GAAGGCCAAG 
CCCAAGGTAC CAGAAAGAAA ATCGTCTCTG ATCTCTTCTA TGTCCATCTC ATCATCGTCC 
ACTTCTCTCT CTTCTAATAC TTCTACCGAA GGAAGTGGGA CCATGAAGAA ACTGGATACA 
ACCCTGGCCT CGGCCCTTGC CCCTCCCCCT CCTCCTCTTC CCCCTCTGCC TTCTCCGTGT 
CTGGCAGACA AGTCCCCTTT TCTTCCACCA CCTCCTCCTC TAGCAGATTG CTCCGAGGGC 
TCTCCTCTGC CGCCCTCTCC CATGTTCCCC CCTCCACCAC CAGAAGCCCT TGTTCCCTTC 
TGCTCCCCCA CCGACGGGTG CCTTTCTCCT TCTCCCACAG CAGTTAGCCC CTCTCTTCCA 
AGATCCTTGC CTCCTGTGCC AGCACCTCCA CCTTTCTTGC CCTCATCAGA ACCCCCGCCC 
GCTCCGCCTC TGGACCCCAA ACTCATGAAA GAAAACAGGC CCTTTTTCAA AAACTCTAGC 
CAGTCCGAAT CCTCTAGGGA GGCCCTCAGG CGGCCTGCCA ACAAGGAGGA GGGCTGCCGA 
CCCCCCATGC CCCTGATCAC CACAGAAGCT TTGCAGATGG TGCAGTTGAG GCCAGTGAGG 
AAGAACTCAG GTGCCGGGGC AGTCCTGTTT TCTGAACCAT CAGCTCAGGA ACAACGAACT 
CCGACTGCTC CACAGTATCA CTTAAAGCCA TCTGCTTTCC TCAAATCCCG AAATAGCATA 
AATGAAATGG AGAGTGAAAG CCAGGCTGCC TCTGTGACGA GCTCGCTTCC GATGCCTGCC 
AAGAGCCAGA GTCAGGGTGA CCATGACAGT GCAGTAGAGC GTGGCGGCCT TCCGAGCTGC 
AGCGATGGAG CTCCAGGCCC GGGTCCCAGC CTCAGGACCA CTCTGCTCCC AGACTCTTCA 
CCCAGCAGGA AGCCACCCCC CATCTCTAAG AAGCCCAAAC TGTTCCTGGT GGTACCACCT 
CCGCAGAGAG ATTTCACAGC GGAGCCCACA GAGAACGGGA GCGAAGCTTT CCCAGGCGTG 
CCCAGCCCTA CCAGGGCAGA GGGGGAAGCT GTGCGGAGCC AAGAGGAGAA ATCCAGCCCA 
GCATCCCGAG CTGGCTCTCA TGCTACGGCC CCCACCCCCG GCAGTCCGGC TCTAGAACCG 
GGAACTGCAG GGTCCTTATC CTCTAGCATT GTGGAAGCCA ATGTCCCCAT GGTCCAACCT 
AATACATCAC CGGGCCCCAC ACAGGAAGAG TCAGGCGAGA ACAGTGTGGA TGGTGAGAGA 
AATGCGAAGA GCTGCCTGTC TCAACAGGGC AGAGAAGCTG GCTTGCTGGA ACCCAACACA 
GCTGCTTCCT CTTCAGACCC CGTGGATGTA TCTAAGGAAG AGGGAAGCGA TGAGGTGCTG 
ACTCCGACTA AACCCAGGAC AACAGAAGAC CTGTTTGCAG CCATTCACAG ATCTAAAAGG 
AAAGTCCTTG GCCGGAAAGA CTCAGAAGAC GATCACACTC GAAATCACTC TCCCTCCCCG 
CCTGTGACAC CCACGAGTGC TGCCCCTAAC CTGGCCTCTC CAAAGCAAGT AGGGTCTATC 
CAGAGAAGCA TAAAAAAGAG CACTACCAGC AGTGACAACT TCAAAGCTCT GCTGCTAAAG 
AAGGGGAGTC GCTCGGACAC CAGCGCTCGC ATGTCAGCGG CTGAGATGCT CAAGTCTACG 
GACCCTCGCT TCCAGAGATC TAGGTCGGAG CCTTCGGCAG ACAGCCCGGA CAGCCCATCC 
AGCTGCTCCC CCAACAAGAA CAGGAGAGCT CAGGAAGAGT GGGCCAAGAA CGAAGGCCTG 
ATGCCTCGGA GTCTGTCCTT TTCGGGCCCT CGGTACAGCC GCAGCAGGAC GCCACCCTCT 
GCGGCCAGCA GCAGGTACAG CATGCGGAAC CGCATCCAGA GCAGCCCCAT GACCGTCATC 
TCCGAGGGAG AGGGAGAACC TGCTGAGCCA GCGGACAACA AAGCACGCAG GGCCCTGGAT 
GCTACCAGGG TGTGCTCTCT GGACAGACTG ACGGGTCAGG AGATGGACCA GGCCAGCCTC 
CTCTGTAGCG AGGAGCCTGC CTCTGTGGAT GGGATAGGAA GGGCTGAGGG CAACGGTCCC 
TCGGAGCAGT GTGGGGGTAC GGAACAGAAG AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006512688.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512751.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu43845
Clone ID Related Accession (Same CDS sequence)	XM_006512688.3 , XM_006512688.4
Accession Version	XM_006512688.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512688.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG 
AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT 
CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA 
GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGCATCAGC CACTGGCCAA 
GATGATGATG GTAGTGCCCA TTCGCTGTAC GTCCCAGACC ACTACTCTAC GTTAGGAAGG 
CTTGATAGTT ATCGGTCCAC CGGGCAATGC TTGGAAACCA GGGACACCAG CTGTCAGACC 
GAAGACGTGA AGGTCATCCC ACCATCAATG AGAAGGATCA GGGCTCACAA GGGGGTAGGC 
GTTGCTGCCC AGATGAGCCA CCTCTCAGGT TCCTCAGGCA ACATGTCTGT GCTGAGTGAC 
TCAGCCGGTG TTGTGTTCCC ATCTCGCCTC AGTAACGATA CTGGTTTCCA CAGTCTTCCA 
CGTACCGGCC CGAGAGCAAG CACCTATTCA CTGGAGGGAA GGATGGGTGC CCTGGGCTCT 
ACTGAAGATA CGGATGACAC TTCTCCCTAC CAGGGGGGTA GCTTACAGGG GCATGAAAAC 
TTTGCGCATT TAGGAGGTGC GTCGAGTACT GGGATGCTTT CGAGGCCCAA ATCCCAGCAG 
TTGAGGTTTT TGGAGAGTCC AGCATGTGTG GTTTCACCTC ATGCAGCCTA CTCTACCAGT 
GTCATCCCAA ATGCCACGCT ACTGTCCTCT TCAGAGGTTA TTGTCATTCA CACTGCTCAG 
AGTGCAGGAC AGCTGGACAG TAGAACACCC GGTTCATCTT CATATTCAAA AATAAAACCC 
AGAGACCGTC CCACACCCAG ATGTTCTGTG AAAGATGACC ATCAGTCCCC CCGTCATCAC 
TGGAACGAGG GTCATCTCAT TCATTCACGG GCTTTAGCCT CCTCTGTCCC TGGTGCCACC 
ACGCTGTTAT CCCTCCATGA TTCAGAGGTC TCTCTAAATG CCCCAGCAAA CAGGGAAAAT 
GGATCCCAAG CCATACTCTA TCATTGTAGA AACAACCCAA GCTTTCCTGA CCACCCTTCA 
GATGTGGATG GCAAGAGCGA GTGTAGTTAT TCGGGGGACA GGGGATGTGG CAGTTCTGAG 
CCCTGGGAAT ATAAAACCTC CAGCAATGGG CGGGCATCCC CACTGAAGCC ACACCTGGCC 
ACTCCTGGTT GCTCTACTCC CACAAGTAAC GTGAGCAGCT GCAGTTTGGA CCAAACATCT 
CTCAAGGGTG ATACCAGGTC CCTGTGTTCA GAGGACCATG ATGGTTACTA TACTACCACT 
CACGAGGCTG GGAACCTGTA CACTCTCAGT GATGGCTTGG GGAACCCCAG GCACAGCATG 
GTCAATGTTT TTGATGGAAG GGCTCAGAGA AGCCAAGGGG ATCAGGCCGC TCACCAGGAT 
AAAATCCTTT CGAGAAACAT CTCTCTGAAG AAAGCAAAGA AACCACCCCT GCCACCATCC 
CGGACCGATT CTCTGCGAAG GATTCCCAAA AAGAACAACC AGACAAACGG TCAGGTGCTC 
AATGAGAGCC TGATCGCCTC GCTCCAGCAT TCACTGCAGC TAAGCCTCCC TGGCAAGGGC 
GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT GACTTTGAGG AACCCTGGCT CCCCAGGTCC 
AGAAGCCAGA GCATTGTTAG CGAGGGTAGT AGCTTGACCT CCACCACCAC TCCCAACGTG 
TATTCTCTGT GTGGGGTCAC TCCGTCCCAG AGCGACACAA GTAGCGTCAA GTCGGAATAC 
ACGGACCCTT GGGGTTACTA CATTGACTAC ACGAGCTTGC AGGAAGATCC AGGGAACCCC 
ACCGGGGGCT GCTCAGCCAA CACTGAGGCA GCAACTGGAA ACGGGCCAGT ACGCCACATC 
CAGGAGGGGT CGAGAGTCCC AGTGCCCCAA GTGCCGGGCT GCTCAGTTAG ACCAAAGATC 
GCCTCACCAG AGAAGTCACA GAGAGTCACC TCTCCATCCA GTGGGTATTC TAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAATGTC ACCAGCAAAT 
GGAAAGGGGA AGGCCAAGCC CAAGGTACCA GAAAGAAAAT CGTCTCTGAT CTCTTCTATG 
TCCATCTCAT CATCGTCCAC TTCTCTCTCT TCTAATACTT CTACCGAAGG AAGTGGGACC 
ATGAAGAAAC TGGATACAAC CCTGGCCTCG GCCCTTGCCC CTCCCCCTCC TCCTCTTCCC 
CCTCTGCCTT CTCCGTGTCT GGCAGACAAG TCCCCTTTTC TTCCACCACC TCCTCCTCTA 
GCAGATTGCT CCGAGGGCTC TCCTCTGCCG CCCTCTCCCA TGTTCCCCCC TCCACCACCA 
GAAGCCCTTG TTCCCTTCTG CTCCCCCACC GACGGGTGCC TTTCTCCTTC TCCCACAGCA 
GTTAGCCCCT CTCTTCCAAG ATCCTTGCCT CCTGTGCCAG CACCTCCACC TTTCTTGCCC 
TCATCAGAAC CCCCGCCCGC TCCGCCTCTG GACCCCAAAC TCATGAAAGA AAACAGGCCC 
TTTTTCAAAA ACTCTAGCCA GTCCGAATCC TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC 
AAGGAGGAGG GCTGCCGACC CCCCATGCCC CTGATCACCA CAGAAGCTTT GCAGATGGTG 
CAGTTGAGGC CAGTGAGGAA GAACTCAGGT GCCGGGGCAG TCCTGTTTTC TGAACCATCA 
GCTCAGGAAC AACGAACTCC GACTGCTCCA CAGTATCACT TAAAGCCATC TGCTTTCCTC 
AAATCCCGAA ATAGCATAAA TGAAATGGAG AGTGAAAGCC AGGCTGCCTC TGTGACGAGC 
TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT CAGGGTGACC ATGACAGTGC AGTAGAGCGT 
GGCGGCCTTC CGAGCTGCAG CGATGGAGCT CCAGGCCCGG GTCCCAGCCT CAGGACCACT 
CTGCTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TCTCTAAGAA GCCCAAACTG 
TTCCTGGTGG TACCACCTCC GCAGAGAGAT TTCACAGCGG AGCCCACAGA GAACGGGAGC 
GAAGCTTTCC CAGGCGTGCC CAGCCCTACC AGGGCAGAGG GGGAAGCTGT GCGGAGCCAA 
GAGGAGAAAT CCAGCCCAGC ATCCCGAGCT GGCTCTCATG CTACGGCCCC CACCCCCGGC 
AGTCCGGCTC TAGAACCGGG AACTGCAGGG TCCTTATCCT CTAGCATTGT GGAAGCCAAT 
GTCCCCATGG TCCAACCTAA TACATCACCG GGCCCCACAC AGGAAGAGTC AGGCGAGAAC 
AGTGTGGATG GTGAGAGAAA TGCGAAGAGC TGCCTGTCTC AACAGGGCAG AGAAGCTGGC 
TTGCTGGAAC CCAACACAGC TGCTTCCTCT TCAGACCCCG TGGATGTATC TAAGGAAGAG 
GGAAGCGATG AGGTGCTGAC TCCGACTAAA CCCAGGACAA CAGAAGACCT GTTTGCAGCC 
ATTCACAGAT CTAAAAGGAA AGTCCTTGGC CGGAAAGACT CAGAAGACGA TCACACTCGA 
AATCACTCTC CCTCCCCGCC TGTGACACCC ACGAGTGCTG CCCCTAACCT GGCCTCTCCA 
AAGCAAGTAG GGTCTATCCA GAGAAGCATA AAAAAGAGCA CTACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTAAAGAA GGGGAGTCGC TCGGACACCA GCGCTCGCAT GTCAGCGGCT 
GAGATGCTCA AGTCTACGGA CCCTCGCTTC CAGAGATCTA GGTCGGAGCC TTCGGCAGAC 
AGCCCGGACA GCCCATCCAG CTGCTCCCCC AACAAGAACA GGAGAGCTCA GGAAGAGTGG 
GCCAAGAACG AAGGCCTGAT GCCTCGGAGT CTGTCCTTTT CGGGCCCTCG GTACAGCCGC 
AGCAGGACGC CACCCTCTGC GGCCAGCAGC AGGTACAGCA TGCGGAACCG CATCCAGAGC 
AGCCCCATGA CCGTCATCTC CGAGGGAGAG GGAGAACCTG CTGAGCCAGC GGACAACAAA 
GCACGCAGGG CCCTGGATGC TACCAGGGTG TGCTCTCTGG ACAGACTGAC GGGTCAGGAG 
ATGGACCAGG CCAGCCTCCT CTGTAGCGAG GAGCCTGCCT CTGTGGATGG GATAGGAAGG 
GCTGAGGGCA ACGGTCCCTC GGAGCAGTGT GGGGGTACGG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512751.1
CDS	422..5275
Translation	MPFHQRSVEPARLRRPDEAALARAGDPPLFRSLEQVSSHTLVCLLAQLADLSRCAGDIFGELEGQAAALGHRTAELHRRLDALHAAAARLDHRRVKIPVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_006512688.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512688.3

ORF Insert Sequence:

ATGCCCTTCC ACCAACGAAG CGTAGAGCCC GCACGGCTGC GCCGGCCCGA CGAGGCGGCA 
CTGGCTCGGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCC TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC TGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCCGCCGC CGCGCGTCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTGGACG AGGAGAGTCG ATGGACTGTC CACTACACGG CACCATGGCA TCAGCAAGAG 
AATGTGTTCC TTCCCGCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCCAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCAAGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCGGCT CCAGCCCCTG CGACGATTAT 
CAAGATGAAG ACACAGAAGC AGACCGAAAG TGCTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATCGGAA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCCGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC GCGGCAGCAA 
GCCCAGACGG TCCAGGCTGA CGTGGTTCCT ATAAACATAA CTGCATCAGC CACTGGCCAA 
GATGATGATG GTAGTGCCCA TTCGCTGTAC GTCCCAGACC ACTACTCTAC GTTAGGAAGG 
CTTGATAGTT ATCGGTCCAC CGGGCAATGC TTGGAAACCA GGGACACCAG CTGTCAGACC 
GAAGACGTGA AGGTCATCCC ACCATCAATG AGAAGGATCA GGGCTCACAA GGGGGTAGGC 
GTTGCTGCCC AGATGAGCCA CCTCTCAGGT TCCTCAGGCA ACATGTCTGT GCTGAGTGAC 
TCAGCCGGTG TTGTGTTCCC ATCTCGCCTC AGTAACGATA CTGGTTTCCA CAGTCTTCCA 
CGTACCGGCC CGAGAGCAAG CACCTATTCA CTGGAGGGAA GGATGGGTGC CCTGGGCTCT 
ACTGAAGATA CGGATGACAC TTCTCCCTAC CAGGGGGGTA GCTTACAGGG GCATGAAAAC 
TTTGCGCATT TAGGAGGTGC GTCGAGTACT GGGATGCTTT CGAGGCCCAA ATCCCAGCAG 
TTGAGGTTTT TGGAGAGTCC AGCATGTGTG GTTTCACCTC ATGCAGCCTA CTCTACCAGT 
GTCATCCCAA ATGCCACGCT ACTGTCCTCT TCAGAGGTTA TTGTCATTCA CACTGCTCAG 
AGTGCAGGAC AGCTGGACAG TAGAACACCC GGTTCATCTT CATATTCAAA AATAAAACCC 
AGAGACCGTC CCACACCCAG ATGTTCTGTG AAAGATGACC ATCAGTCCCC CCGTCATCAC 
TGGAACGAGG GTCATCTCAT TCATTCACGG GCTTTAGCCT CCTCTGTCCC TGGTGCCACC 
ACGCTGTTAT CCCTCCATGA TTCAGAGGTC TCTCTAAATG CCCCAGCAAA CAGGGAAAAT 
GGATCCCAAG CCATACTCTA TCATTGTAGA AACAACCCAA GCTTTCCTGA CCACCCTTCA 
GATGTGGATG GCAAGAGCGA GTGTAGTTAT TCGGGGGACA GGGGATGTGG CAGTTCTGAG 
CCCTGGGAAT ATAAAACCTC CAGCAATGGG CGGGCATCCC CACTGAAGCC ACACCTGGCC 
ACTCCTGGTT GCTCTACTCC CACAAGTAAC GTGAGCAGCT GCAGTTTGGA CCAAACATCT 
CTCAAGGGTG ATACCAGGTC CCTGTGTTCA GAGGACCATG ATGGTTACTA TACTACCACT 
CACGAGGCTG GGAACCTGTA CACTCTCAGT GATGGCTTGG GGAACCCCAG GCACAGCATG 
GTCAATGTTT TTGATGGAAG GGCTCAGAGA AGCCAAGGGG ATCAGGCCGC TCACCAGGAT 
AAAATCCTTT CGAGAAACAT CTCTCTGAAG AAAGCAAAGA AACCACCCCT GCCACCATCC 
CGGACCGATT CTCTGCGAAG GATTCCCAAA AAGAACAACC AGACAAACGG TCAGGTGCTC 
AATGAGAGCC TGATCGCCTC GCTCCAGCAT TCACTGCAGC TAAGCCTCCC TGGCAAGGGC 
GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT GACTTTGAGG AACCCTGGCT CCCCAGGTCC 
AGAAGCCAGA GCATTGTTAG CGAGGGTAGT AGCTTGACCT CCACCACCAC TCCCAACGTG 
TATTCTCTGT GTGGGGTCAC TCCGTCCCAG AGCGACACAA GTAGCGTCAA GTCGGAATAC 
ACGGACCCTT GGGGTTACTA CATTGACTAC ACGAGCTTGC AGGAAGATCC AGGGAACCCC 
ACCGGGGGCT GCTCAGCCAA CACTGAGGCA GCAACTGGAA ACGGGCCAGT ACGCCACATC 
CAGGAGGGGT CGAGAGTCCC AGTGCCCCAA GTGCCGGGCT GCTCAGTTAG ACCAAAGATC 
GCCTCACCAG AGAAGTCACA GAGAGTCACC TCTCCATCCA GTGGGTATTC TAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAATGTC ACCAGCAAAT 
GGAAAGGGGA AGGCCAAGCC CAAGGTACCA GAAAGAAAAT CGTCTCTGAT CTCTTCTATG 
TCCATCTCAT CATCGTCCAC TTCTCTCTCT TCTAATACTT CTACCGAAGG AAGTGGGACC 
ATGAAGAAAC TGGATACAAC CCTGGCCTCG GCCCTTGCCC CTCCCCCTCC TCCTCTTCCC 
CCTCTGCCTT CTCCGTGTCT GGCAGACAAG TCCCCTTTTC TTCCACCACC TCCTCCTCTA 
GCAGATTGCT CCGAGGGCTC TCCTCTGCCG CCCTCTCCCA TGTTCCCCCC TCCACCACCA 
GAAGCCCTTG TTCCCTTCTG CTCCCCCACC GACGGGTGCC TTTCTCCTTC TCCCACAGCA 
GTTAGCCCCT CTCTTCCAAG ATCCTTGCCT CCTGTGCCAG CACCTCCACC TTTCTTGCCC 
TCATCAGAAC CCCCGCCCGC TCCGCCTCTG GACCCCAAAC TCATGAAAGA AAACAGGCCC 
TTTTTCAAAA ACTCTAGCCA GTCCGAATCC TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC 
AAGGAGGAGG GCTGCCGACC CCCCATGCCC CTGATCACCA CAGAAGCTTT GCAGATGGTG 
CAGTTGAGGC CAGTGAGGAA GAACTCAGGT GCCGGGGCAG TCCTGTTTTC TGAACCATCA 
GCTCAGGAAC AACGAACTCC GACTGCTCCA CAGTATCACT TAAAGCCATC TGCTTTCCTC 
AAATCCCGAA ATAGCATAAA TGAAATGGAG AGTGAAAGCC AGGCTGCCTC TGTGACGAGC 
TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT CAGGGTGACC ATGACAGTGC AGTAGAGCGT 
GGCGGCCTTC CGAGCTGCAG CGATGGAGCT CCAGGCCCGG GTCCCAGCCT CAGGACCACT 
CTGCTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TCTCTAAGAA GCCCAAACTG 
TTCCTGGTGG TACCACCTCC GCAGAGAGAT TTCACAGCGG AGCCCACAGA GAACGGGAGC 
GAAGCTTTCC CAGGCGTGCC CAGCCCTACC AGGGCAGAGG GGGAAGCTGT GCGGAGCCAA 
GAGGAGAAAT CCAGCCCAGC ATCCCGAGCT GGCTCTCATG CTACGGCCCC CACCCCCGGC 
AGTCCGGCTC TAGAACCGGG AACTGCAGGG TCCTTATCCT CTAGCATTGT GGAAGCCAAT 
GTCCCCATGG TCCAACCTAA TACATCACCG GGCCCCACAC AGGAAGAGTC AGGCGAGAAC 
AGTGTGGATG GTGAGAGAAA TGCGAAGAGC TGCCTGTCTC AACAGGGCAG AGAAGCTGGC 
TTGCTGGAAC CCAACACAGC TGCTTCCTCT TCAGACCCCG TGGATGTATC TAAGGAAGAG 
GGAAGCGATG AGGTGCTGAC TCCGACTAAA CCCAGGACAA CAGAAGACCT GTTTGCAGCC 
ATTCACAGAT CTAAAAGGAA AGTCCTTGGC CGGAAAGACT CAGAAGACGA TCACACTCGA 
AATCACTCTC CCTCCCCGCC TGTGACACCC ACGAGTGCTG CCCCTAACCT GGCCTCTCCA 
AAGCAAGTAG GGTCTATCCA GAGAAGCATA AAAAAGAGCA CTACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTAAAGAA GGGGAGTCGC TCGGACACCA GCGCTCGCAT GTCAGCGGCT 
GAGATGCTCA AGTCTACGGA CCCTCGCTTC CAGAGATCTA GGTCGGAGCC TTCGGCAGAC 
AGCCCGGACA GCCCATCCAG CTGCTCCCCC AACAAGAACA GGAGAGCTCA GGAAGAGTGG 
GCCAAGAACG AAGGCCTGAT GCCTCGGAGT CTGTCCTTTT CGGGCCCTCG GTACAGCCGC 
AGCAGGACGC CACCCTCTGC GGCCAGCAGC AGGTACAGCA TGCGGAACCG CATCCAGAGC 
AGCCCCATGA CCGTCATCTC CGAGGGAGAG GGAGAACCTG CTGAGCCAGC GGACAACAAA 
GCACGCAGGG CCCTGGATGC TACCAGGGTG TGCTCTCTGG ACAGACTGAC GGGTCAGGAG 
ATGGACCAGG CCAGCCTCCT CTGTAGCGAG GAGCCTGCCT CTGTGGATGG GATAGGAAGG 
GCTGAGGGCA ACGGTCCCTC GGAGCAGTGT GGGGGTACGG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006512689.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512752.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.

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CloneID	OMu43846
Clone ID Related Accession (Same CDS sequence)	XM_006512689.3 , XM_006512689.4
Accession Version	XM_006512689.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512689.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATG CAATATACCG TCTGGAGCCT GTGGCTGATT CCGCCATCCA GTGGGGTGCC 
CTTGCGGTTT CCAACTTGGA CGAGGAGAGT CGATGGACTG TCCACTACAC GGCACCATGG 
CATCAGCAAG AGAATGTGTT CCTTCCCGCC ACAAGACCCC CCTGTGTTGA GGACCTGCAC 
CGCCAAGCCA AGCTCAACCT CAAATCAGTG CTGAGGGAAT GTGACAAGCT GAGGCAAGAT 
GGCTGCCGAA GTTCTCAGTA CTATTCTCAG GGACCCACCT TTGCAGCCGG CTCCAGCCCC 
TGCGACGATT ATCAAGATGA AGACACAGAA GCAGACCGAA AGTGCTCTCT CTCTTCATCT 
GAAGAAGAAA GATTTATCGG AATCAGGAGA CCTAAAACGC CAACCTCAGG TGACTTCTCC 
GACCTTCATA CTCAGACAAA CTGGACCAAG TCCCTGCCGC TGCCAACACC AGAAGAGAAG 
ACGCGGCAGC AAGCCCAGAC GGTCCAGGCT GACGTGGTTC CTATAAACAT AACTGGGGAG 
AATTTCGATC GTCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACA ATTTCAGGAA TCCCTGACAT CATTCAGAAG 
GAGCTAGCAT CAGCCACTGG CCAAGATGAT GATGGTAGTG CCCATTCGCT GTACGTCCCA 
GACCACTACT CTACGTTAGG AAGGCTTGAT AGTTATCGGT CCACCGGGCA ATGCTTGGAA 
ACCAGGGACA CCAGCTGTCA GACCGAAGAC GTGAAGGTCA TCCCACCATC AATGAGAAGG 
ATCAGGGCTC ACAAGGGGGT AGGCGTTGCT GCCCAGATGA GCCACCTCTC AGGTTCCTCA 
GGCAACATGT CTGTGCTGAG TGACTCAGCC GGTGTTGTGT TCCCATCTCG CCTCAGTAAC 
GATACTGGTT TCCACAGTCT TCCACGTACC GGCCCGAGAG CAAGCACCTA TTCACTGGAG 
GGAAGGATGG GTGCCCTGGG CTCTACTGAA GATACGGATG ACACTTCTCC CTACCAGGGG 
GGTAGCTTAC AGGGGCATGA AAACTTTGCG CATTTAGGAG GTGCGTCGAG TACTGGGATG 
CTTTCGAGGC CCAAATCCCA GCAGTTGAGG TTTTTGGAGA GTCCAGCATG TGTGGTTTCA 
CCTCATGCAG CCTACTCTAC CAGTGTCATC CCAAATGCCA CGCTACTGTC CTCTTCAGAG 
GTTATTGTCA TTCACACTGC TCAGAGTGCA GGACAGCTGG ACAGTAGAAC ACCCGGTTCA 
TCTTCATATT CAAAAATAAA ACCCAGAGAC CGTCCCACAC CCAGATGTTC TGTGAAAGAT 
GACCATCAGT CCCCCCGTCA TCACTGGAAC GAGGGTCATC TCATTCATTC ACGGGCTTTA 
GCCTCCTCTG TCCCTGGTGC CACCACGCTG TTATCCCTCC ATGATTCAGA GGTCTCTCTA 
AATGCCCCAG CAAACAGGGA AAATGGATCC CAAGCCATAC TCTATCATTG TAGAAACAAC 
CCAAGCTTTC CTGACCACCC TTCAGATGTG GATGGCAAGA GCGAGTGTAG TTATTCGGGG 
GACAGGGGAT GTGGCAGTTC TGAGCCCTGG GAATATAAAA CCTCCAGCAA TGGGCGGGCA 
TCCCCACTGA AGCCACACCT GGCCACTCCT GGTTGCTCTA CTCCCACAAG TAACGTGAGC 
AGCTGCAGTT TGGACCAAAC ATCTCTCAAG GGTGATACCA GGTCCCTGTG TTCAGAGGAC 
CATGATGGTT ACTATACTAC CACTCACGAG GCTGGGAACC TGTACACTCT CAGTGATGGC 
TTGGGGAACC CCAGGCACAG CATGGTCAAT GTTTTTGATG GAAGGGCTCA GAGAAGCCAA 
GGGGATCAGG CCGCTCACCA GGATAAAATC CTTTCGAGAA ACATCTCTCT GAAGAAAGCA 
AAGAAACCAC CCCTGCCACC ATCCCGGACC GATTCTCTGC GAAGGATTCC CAAAAAGAAC 
AACCAGACAA ACGGTCAGGT GCTCAATGAG AGCCTGATCG CCTCGCTCCA GCATTCACTG 
CAGCTAAGCC TCCCTGGCAA GGGCGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTTT 
GAGGAACCCT GGCTCCCCAG GTCCAGAAGC CAGAGCATTG TTAGCGAGGG TAGTAGCTTG 
ACCTCCACCA CCACTCCCAA CGTGTATTCT CTGTGTGGGG TCACTCCGTC CCAGAGCGAC 
ACAAGTAGCG TCAAGTCGGA ATACACGGAC CCTTGGGGTT ACTACATTGA CTACACGAGC 
TTGCAGGAAG ATCCAGGGAA CCCCACCGGG GGCTGCTCAG CCAACACTGA GGCAGCAACT 
GGAAACGGGC CAGTACGCCA CATCCAGGAG GGGTCGAGAG TCCCAGTGCC CCAAGTGCCG 
GGCTGCTCAG TTAGACCAAA GATCGCCTCA CCAGAGAAGT CACAGAGAGT CACCTCTCCA 
TCCAGTGGGT ATTCTAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT 
TTAAAATCAA TGTCACCAGC AAATGGAAAG GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA 
AAATCGTCTC TGATCTCTTC TATGTCCATC TCATCATCGT CCACTTCTCT CTCTTCTAAT 
ACTTCTACCG AAGGAAGTGG GACCATGAAG AAACTGGATA CAACCCTGGC CTCGGCCCTT 
GCCCCTCCCC CTCCTCCTCT TCCCCCTCTG CCTTCTCCGT GTCTGGCAGA CAAGTCCCCT 
TTTCTTCCAC CACCTCCTCC TCTAGCAGAT TGCTCCGAGG GCTCTCCTCT GCCGCCCTCT 
CCCATGTTCC CCCCTCCACC ACCAGAAGCC CTTGTTCCCT TCTGCTCCCC CACCGACGGG 
TGCCTTTCTC CTTCTCCCAC AGCAGTTAGC CCCTCTCTTC CAAGATCCTT GCCTCCTGTG 
CCAGCACCTC CACCTTTCTT GCCCTCATCA GAACCCCCGC CCGCTCCGCC TCTGGACCCC 
AAACTCATGA AAGAAAACAG GCCCTTTTTC AAAAACTCTA GCCAGTCCGA ATCCTCTAGG 
GAGGCCCTCA GGCGGCCTGC CAACAAGGAG GAGGGCTGCC GACCCCCCAT GCCCCTGATC 
ACCACAGAAG CTTTGCAGAT GGTGCAGTTG AGGCCAGTGA GGAAGAACTC AGGTGCCGGG 
GCAGTCCTGT TTTCTGAACC ATCAGCTCAG GAACAACGAA CTCCGACTGC TCCACAGTAT 
CACTTAAAGC CATCTGCTTT CCTCAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA 
AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT CCGATGCCTG CCAAGAGCCA GAGTCAGGGT 
GACCATGACA GTGCAGTAGA GCGTGGCGGC CTTCCGAGCT GCAGCGATGG AGCTCCAGGC 
CCGGGTCCCA GCCTCAGGAC CACTCTGCTC CCAGACTCTT CACCCAGCAG GAAGCCACCC 
CCCATCTCTA AGAAGCCCAA ACTGTTCCTG GTGGTACCAC CTCCGCAGAG AGATTTCACA 
GCGGAGCCCA CAGAGAACGG GAGCGAAGCT TTCCCAGGCG TGCCCAGCCC TACCAGGGCA 
GAGGGGGAAG CTGTGCGGAG CCAAGAGGAG AAATCCAGCC CAGCATCCCG AGCTGGCTCT 
CATGCTACGG CCCCCACCCC CGGCAGTCCG GCTCTAGAAC CGGGAACTGC AGGGTCCTTA 
TCCTCTAGCA TTGTGGAAGC CAATGTCCCC ATGGTCCAAC CTAATACATC ACCGGGCCCC 
ACACAGGAAG AGTCAGGCGA GAACAGTGTG GATGGTGAGA GAAATGCGAA GAGCTGCCTG 
TCTCAACAGG GCAGAGAAGC TGGCTTGCTG GAACCCAACA CAGCTGCTTC CTCTTCAGAC 
CCCGTGGATG TATCTAAGGA AGAGGGAAGC GATGAGGTGC TGACTCCGAC TAAACCCAGG 
ACAACAGAAG ACCTGTTTGC AGCCATTCAC AGATCTAAAA GGAAAGTCCT TGGCCGGAAA 
GACTCAGAAG ACGATCACAC TCGAAATCAC TCTCCCTCCC CGCCTGTGAC ACCCACGAGT 
GCTGCCCCTA ACCTGGCCTC TCCAAAGCAA GTAGGGTCTA TCCAGAGAAG CATAAAAAAG 
AGCACTACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AGAAGGGGAG TCGCTCGGAC 
ACCAGCGCTC GCATGTCAGC GGCTGAGATG CTCAAGTCTA CGGACCCTCG CTTCCAGAGA 
TCTAGGTCGG AGCCTTCGGC AGACAGCCCG GACAGCCCAT CCAGCTGCTC CCCCAACAAG 
AACAGGAGAG CTCAGGAAGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC 
TTTTCGGGCC CTCGGTACAG CCGCAGCAGG ACGCCACCCT CTGCGGCCAG CAGCAGGTAC 
AGCATGCGGA ACCGCATCCA GAGCAGCCCC ATGACCGTCA TCTCCGAGGG AGAGGGAGAA 
CCTGCTGAGC CAGCGGACAA CAAAGCACGC AGGGCCCTGG ATGCTACCAG GGTGTGCTCT 
CTGGACAGAC TGACGGGTCA GGAGATGGAC CAGGCCAGCC TCCTCTGTAG CGAGGAGCCT 
GCCTCTGTGG ATGGGATAGG AAGGGCTGAG GGCAACGGTC CCTCGGAGCA GTGTGGGGGT 
ACGGAACAGA AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512752.1
CDS	321..5078
Translation	MMNAIYRLEPVADSAIQWGALAVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_006512689.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512689.3

ORF Insert Sequence:

ATGATGAATG CAATATACCG TCTGGAGCCT GTGGCTGATT CCGCCATCCA GTGGGGTGCC 
CTTGCGGTTT CCAACTTGGA CGAGGAGAGT CGATGGACTG TCCACTACAC GGCACCATGG 
CATCAGCAAG AGAATGTGTT CCTTCCCGCC ACAAGACCCC CCTGTGTTGA GGACCTGCAC 
CGCCAAGCCA AGCTCAACCT CAAATCAGTG CTGAGGGAAT GTGACAAGCT GAGGCAAGAT 
GGCTGCCGAA GTTCTCAGTA CTATTCTCAG GGACCCACCT TTGCAGCCGG CTCCAGCCCC 
TGCGACGATT ATCAAGATGA AGACACAGAA GCAGACCGAA AGTGCTCTCT CTCTTCATCT 
GAAGAAGAAA GATTTATCGG AATCAGGAGA CCTAAAACGC CAACCTCAGG TGACTTCTCC 
GACCTTCATA CTCAGACAAA CTGGACCAAG TCCCTGCCGC TGCCAACACC AGAAGAGAAG 
ACGCGGCAGC AAGCCCAGAC GGTCCAGGCT GACGTGGTTC CTATAAACAT AACTGGGGAG 
AATTTCGATC GTCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACA ATTTCAGGAA TCCCTGACAT CATTCAGAAG 
GAGCTAGCAT CAGCCACTGG CCAAGATGAT GATGGTAGTG CCCATTCGCT GTACGTCCCA 
GACCACTACT CTACGTTAGG AAGGCTTGAT AGTTATCGGT CCACCGGGCA ATGCTTGGAA 
ACCAGGGACA CCAGCTGTCA GACCGAAGAC GTGAAGGTCA TCCCACCATC AATGAGAAGG 
ATCAGGGCTC ACAAGGGGGT AGGCGTTGCT GCCCAGATGA GCCACCTCTC AGGTTCCTCA 
GGCAACATGT CTGTGCTGAG TGACTCAGCC GGTGTTGTGT TCCCATCTCG CCTCAGTAAC 
GATACTGGTT TCCACAGTCT TCCACGTACC GGCCCGAGAG CAAGCACCTA TTCACTGGAG 
GGAAGGATGG GTGCCCTGGG CTCTACTGAA GATACGGATG ACACTTCTCC CTACCAGGGG 
GGTAGCTTAC AGGGGCATGA AAACTTTGCG CATTTAGGAG GTGCGTCGAG TACTGGGATG 
CTTTCGAGGC CCAAATCCCA GCAGTTGAGG TTTTTGGAGA GTCCAGCATG TGTGGTTTCA 
CCTCATGCAG CCTACTCTAC CAGTGTCATC CCAAATGCCA CGCTACTGTC CTCTTCAGAG 
GTTATTGTCA TTCACACTGC TCAGAGTGCA GGACAGCTGG ACAGTAGAAC ACCCGGTTCA 
TCTTCATATT CAAAAATAAA ACCCAGAGAC CGTCCCACAC CCAGATGTTC TGTGAAAGAT 
GACCATCAGT CCCCCCGTCA TCACTGGAAC GAGGGTCATC TCATTCATTC ACGGGCTTTA 
GCCTCCTCTG TCCCTGGTGC CACCACGCTG TTATCCCTCC ATGATTCAGA GGTCTCTCTA 
AATGCCCCAG CAAACAGGGA AAATGGATCC CAAGCCATAC TCTATCATTG TAGAAACAAC 
CCAAGCTTTC CTGACCACCC TTCAGATGTG GATGGCAAGA GCGAGTGTAG TTATTCGGGG 
GACAGGGGAT GTGGCAGTTC TGAGCCCTGG GAATATAAAA CCTCCAGCAA TGGGCGGGCA 
TCCCCACTGA AGCCACACCT GGCCACTCCT GGTTGCTCTA CTCCCACAAG TAACGTGAGC 
AGCTGCAGTT TGGACCAAAC ATCTCTCAAG GGTGATACCA GGTCCCTGTG TTCAGAGGAC 
CATGATGGTT ACTATACTAC CACTCACGAG GCTGGGAACC TGTACACTCT CAGTGATGGC 
TTGGGGAACC CCAGGCACAG CATGGTCAAT GTTTTTGATG GAAGGGCTCA GAGAAGCCAA 
GGGGATCAGG CCGCTCACCA GGATAAAATC CTTTCGAGAA ACATCTCTCT GAAGAAAGCA 
AAGAAACCAC CCCTGCCACC ATCCCGGACC GATTCTCTGC GAAGGATTCC CAAAAAGAAC 
AACCAGACAA ACGGTCAGGT GCTCAATGAG AGCCTGATCG CCTCGCTCCA GCATTCACTG 
CAGCTAAGCC TCCCTGGCAA GGGCGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTTT 
GAGGAACCCT GGCTCCCCAG GTCCAGAAGC CAGAGCATTG TTAGCGAGGG TAGTAGCTTG 
ACCTCCACCA CCACTCCCAA CGTGTATTCT CTGTGTGGGG TCACTCCGTC CCAGAGCGAC 
ACAAGTAGCG TCAAGTCGGA ATACACGGAC CCTTGGGGTT ACTACATTGA CTACACGAGC 
TTGCAGGAAG ATCCAGGGAA CCCCACCGGG GGCTGCTCAG CCAACACTGA GGCAGCAACT 
GGAAACGGGC CAGTACGCCA CATCCAGGAG GGGTCGAGAG TCCCAGTGCC CCAAGTGCCG 
GGCTGCTCAG TTAGACCAAA GATCGCCTCA CCAGAGAAGT CACAGAGAGT CACCTCTCCA 
TCCAGTGGGT ATTCTAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT 
TTAAAATCAA TGTCACCAGC AAATGGAAAG GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA 
AAATCGTCTC TGATCTCTTC TATGTCCATC TCATCATCGT CCACTTCTCT CTCTTCTAAT 
ACTTCTACCG AAGGAAGTGG GACCATGAAG AAACTGGATA CAACCCTGGC CTCGGCCCTT 
GCCCCTCCCC CTCCTCCTCT TCCCCCTCTG CCTTCTCCGT GTCTGGCAGA CAAGTCCCCT 
TTTCTTCCAC CACCTCCTCC TCTAGCAGAT TGCTCCGAGG GCTCTCCTCT GCCGCCCTCT 
CCCATGTTCC CCCCTCCACC ACCAGAAGCC CTTGTTCCCT TCTGCTCCCC CACCGACGGG 
TGCCTTTCTC CTTCTCCCAC AGCAGTTAGC CCCTCTCTTC CAAGATCCTT GCCTCCTGTG 
CCAGCACCTC CACCTTTCTT GCCCTCATCA GAACCCCCGC CCGCTCCGCC TCTGGACCCC 
AAACTCATGA AAGAAAACAG GCCCTTTTTC AAAAACTCTA GCCAGTCCGA ATCCTCTAGG 
GAGGCCCTCA GGCGGCCTGC CAACAAGGAG GAGGGCTGCC GACCCCCCAT GCCCCTGATC 
ACCACAGAAG CTTTGCAGAT GGTGCAGTTG AGGCCAGTGA GGAAGAACTC AGGTGCCGGG 
GCAGTCCTGT TTTCTGAACC ATCAGCTCAG GAACAACGAA CTCCGACTGC TCCACAGTAT 
CACTTAAAGC CATCTGCTTT CCTCAAATCC CGAAATAGCA TAAATGAAAT GGAGAGTGAA 
AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT CCGATGCCTG CCAAGAGCCA GAGTCAGGGT 
GACCATGACA GTGCAGTAGA GCGTGGCGGC CTTCCGAGCT GCAGCGATGG AGCTCCAGGC 
CCGGGTCCCA GCCTCAGGAC CACTCTGCTC CCAGACTCTT CACCCAGCAG GAAGCCACCC 
CCCATCTCTA AGAAGCCCAA ACTGTTCCTG GTGGTACCAC CTCCGCAGAG AGATTTCACA 
GCGGAGCCCA CAGAGAACGG GAGCGAAGCT TTCCCAGGCG TGCCCAGCCC TACCAGGGCA 
GAGGGGGAAG CTGTGCGGAG CCAAGAGGAG AAATCCAGCC CAGCATCCCG AGCTGGCTCT 
CATGCTACGG CCCCCACCCC CGGCAGTCCG GCTCTAGAAC CGGGAACTGC AGGGTCCTTA 
TCCTCTAGCA TTGTGGAAGC CAATGTCCCC ATGGTCCAAC CTAATACATC ACCGGGCCCC 
ACACAGGAAG AGTCAGGCGA GAACAGTGTG GATGGTGAGA GAAATGCGAA GAGCTGCCTG 
TCTCAACAGG GCAGAGAAGC TGGCTTGCTG GAACCCAACA CAGCTGCTTC CTCTTCAGAC 
CCCGTGGATG TATCTAAGGA AGAGGGAAGC GATGAGGTGC TGACTCCGAC TAAACCCAGG 
ACAACAGAAG ACCTGTTTGC AGCCATTCAC AGATCTAAAA GGAAAGTCCT TGGCCGGAAA 
GACTCAGAAG ACGATCACAC TCGAAATCAC TCTCCCTCCC CGCCTGTGAC ACCCACGAGT 
GCTGCCCCTA ACCTGGCCTC TCCAAAGCAA GTAGGGTCTA TCCAGAGAAG CATAAAAAAG 
AGCACTACCA GCAGTGACAA CTTCAAAGCT CTGCTGCTAA AGAAGGGGAG TCGCTCGGAC 
ACCAGCGCTC GCATGTCAGC GGCTGAGATG CTCAAGTCTA CGGACCCTCG CTTCCAGAGA 
TCTAGGTCGG AGCCTTCGGC AGACAGCCCG GACAGCCCAT CCAGCTGCTC CCCCAACAAG 
AACAGGAGAG CTCAGGAAGA GTGGGCCAAG AACGAAGGCC TGATGCCTCG GAGTCTGTCC 
TTTTCGGGCC CTCGGTACAG CCGCAGCAGG ACGCCACCCT CTGCGGCCAG CAGCAGGTAC 
AGCATGCGGA ACCGCATCCA GAGCAGCCCC ATGACCGTCA TCTCCGAGGG AGAGGGAGAA 
CCTGCTGAGC CAGCGGACAA CAAAGCACGC AGGGCCCTGG ATGCTACCAG GGTGTGCTCT 
CTGGACAGAC TGACGGGTCA GGAGATGGAC CAGGCCAGCC TCCTCTGTAG CGAGGAGCCT 
GCCTCTGTGG ATGGGATAGG AAGGGCTGAG GGCAACGGTC CCTCGGAGCA GTGTGGGGGT 
ACGGAACAGA AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006512690.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512753.1 Mus musculus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.

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CloneID	OMu43847
Clone ID Related Accession (Same CDS sequence)	XM_006512690.4 , XM_006512690.3
Accession Version	XM_006512690.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512690.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTGCC TGAAAGCGGT TTCCAACTTG GACGAGGAGA GTCGATGGAC TGTCCACTAC 
ACGGCACCAT GGCATCAGCA AGAGAATGTG TTCCTTCCCG CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG 
CTGAGGCAAG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC 
GGCTCCAGCC CCTGCGACGA TTATCAAGAT GAAGACACAG AAGCAGACCG AAAGTGCTCT 
CTCTCTTCAT CTGAAGAAGA AAGATTTATC GGAATCAGGA GACCTAAAAC GCCAACCTCA 
GGTGACTTCT CCGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA 
CCAGAAGAGA AGACGCGGCA GCAAGCCCAG ACGGTCCAGG CTGACGTGGT TCCTATAAAC 
ATAACTGGGG AGAATTTCGA TCGTCAGGCC AGCCTTCGGC GGTCTCTAAT TTACACAGAC 
ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CAATTTCAGG AATCCCTGAC 
ATCATTCAGA AGGAGCTAGC ATCAGCCACT GGCCAAGATG ATGATGGTAG TGCCCATTCG 
CTGTACGTCC CAGACCACTA CTCTACGTTA GGAAGGCTTG ATAGTTATCG GTCCACCGGG 
CAATGCTTGG AAACCAGGGA CACCAGCTGT CAGACCGAAG ACGTGAAGGT CATCCCACCA 
TCAATGAGAA GGATCAGGGC TCACAAGGGG GTAGGCGTTG CTGCCCAGAT GAGCCACCTC 
TCAGGTTCCT CAGGCAACAT GTCTGTGCTG AGTGACTCAG CCGGTGTTGT GTTCCCATCT 
CGCCTCAGTA ACGATACTGG TTTCCACAGT CTTCCACGTA CCGGCCCGAG AGCAAGCACC 
TATTCACTGG AGGGAAGGAT GGGTGCCCTG GGCTCTACTG AAGATACGGA TGACACTTCT 
CCCTACCAGG GGGGTAGCTT ACAGGGGCAT GAAAACTTTG CGCATTTAGG AGGTGCGTCG 
AGTACTGGGA TGCTTTCGAG GCCCAAATCC CAGCAGTTGA GGTTTTTGGA GAGTCCAGCA 
TGTGTGGTTT CACCTCATGC AGCCTACTCT ACCAGTGTCA TCCCAAATGC CACGCTACTG 
TCCTCTTCAG AGGTTATTGT CATTCACACT GCTCAGAGTG CAGGACAGCT GGACAGTAGA 
ACACCCGGTT CATCTTCATA TTCAAAAATA AAACCCAGAG ACCGTCCCAC ACCCAGATGT 
TCTGTGAAAG ATGACCATCA GTCCCCCCGT CATCACTGGA ACGAGGGTCA TCTCATTCAT 
TCACGGGCTT TAGCCTCCTC TGTCCCTGGT GCCACCACGC TGTTATCCCT CCATGATTCA 
GAGGTCTCTC TAAATGCCCC AGCAAACAGG GAAAATGGAT CCCAAGCCAT ACTCTATCAT 
TGTAGAAACA ACCCAAGCTT TCCTGACCAC CCTTCAGATG TGGATGGCAA GAGCGAGTGT 
AGTTATTCGG GGGACAGGGG ATGTGGCAGT TCTGAGCCCT GGGAATATAA AACCTCCAGC 
AATGGGCGGG CATCCCCACT GAAGCCACAC CTGGCCACTC CTGGTTGCTC TACTCCCACA 
AGTAACGTGA GCAGCTGCAG TTTGGACCAA ACATCTCTCA AGGGTGATAC CAGGTCCCTG 
TGTTCAGAGG ACCATGATGG TTACTATACT ACCACTCACG AGGCTGGGAA CCTGTACACT 
CTCAGTGATG GCTTGGGGAA CCCCAGGCAC AGCATGGTCA ATGTTTTTGA TGGAAGGGCT 
CAGAGAAGCC AAGGGGATCA GGCCGCTCAC CAGGATAAAA TCCTTTCGAG AAACATCTCT 
CTGAAGAAAG CAAAGAAACC ACCCCTGCCA CCATCCCGGA CCGATTCTCT GCGAAGGATT 
CCCAAAAAGA ACAACCAGAC AAACGGTCAG GTGCTCAATG AGAGCCTGAT CGCCTCGCTC 
CAGCATTCAC TGCAGCTAAG CCTCCCTGGC AAGGGCGGCA GCTCCCCTTC CCAGAGCCCC 
TGCAGTGACT TTGAGGAACC CTGGCTCCCC AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG 
GGTAGTAGCT TGACCTCCAC CACCACTCCC AACGTGTATT CTCTGTGTGG GGTCACTCCG 
TCCCAGAGCG ACACAAGTAG CGTCAAGTCG GAATACACGG ACCCTTGGGG TTACTACATT 
GACTACACGA GCTTGCAGGA AGATCCAGGG AACCCCACCG GGGGCTGCTC AGCCAACACT 
GAGGCAGCAA CTGGAAACGG GCCAGTACGC CACATCCAGG AGGGGTCGAG AGTCCCAGTG 
CCCCAAGTGC CGGGCTGCTC AGTTAGACCA AAGATCGCCT CACCAGAGAA GTCACAGAGA 
GTCACCTCTC CATCCAGTGG GTATTCTAGC CAGTCGAATA CACCCACAGC ACTCACCCCT 
GTGCCTGTGT TTTTAAAATC AATGTCACCA GCAAATGGAA AGGGGAAGGC CAAGCCCAAG 
GTACCAGAAA GAAAATCGTC TCTGATCTCT TCTATGTCCA TCTCATCATC GTCCACTTCT 
CTCTCTTCTA ATACTTCTAC CGAAGGAAGT GGGACCATGA AGAAACTGGA TACAACCCTG 
GCCTCGGCCC TTGCCCCTCC CCCTCCTCCT CTTCCCCCTC TGCCTTCTCC GTGTCTGGCA 
GACAAGTCCC CTTTTCTTCC ACCACCTCCT CCTCTAGCAG ATTGCTCCGA GGGCTCTCCT 
CTGCCGCCCT CTCCCATGTT CCCCCCTCCA CCACCAGAAG CCCTTGTTCC CTTCTGCTCC 
CCCACCGACG GGTGCCTTTC TCCTTCTCCC ACAGCAGTTA GCCCCTCTCT TCCAAGATCC 
TTGCCTCCTG TGCCAGCACC TCCACCTTTC TTGCCCTCAT CAGAACCCCC GCCCGCTCCG 
CCTCTGGACC CCAAACTCAT GAAAGAAAAC AGGCCCTTTT TCAAAAACTC TAGCCAGTCC 
GAATCCTCTA GGGAGGCCCT CAGGCGGCCT GCCAACAAGG AGGAGGGCTG CCGACCCCCC 
ATGCCCCTGA TCACCACAGA AGCTTTGCAG ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC 
TCAGGTGCCG GGGCAGTCCT GTTTTCTGAA CCATCAGCTC AGGAACAACG AACTCCGACT 
GCTCCACAGT ATCACTTAAA GCCATCTGCT TTCCTCAAAT CCCGAAATAG CATAAATGAA 
ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG ACGAGCTCGC TTCCGATGCC TGCCAAGAGC 
CAGAGTCAGG GTGACCATGA CAGTGCAGTA GAGCGTGGCG GCCTTCCGAG CTGCAGCGAT 
GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG ACCACTCTGC TCCCAGACTC TTCACCCAGC 
AGGAAGCCAC CCCCCATCTC TAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG 
AGAGATTTCA CAGCGGAGCC CACAGAGAAC GGGAGCGAAG CTTTCCCAGG CGTGCCCAGC 
CCTACCAGGG CAGAGGGGGA AGCTGTGCGG AGCCAAGAGG AGAAATCCAG CCCAGCATCC 
CGAGCTGGCT CTCATGCTAC GGCCCCCACC CCCGGCAGTC CGGCTCTAGA ACCGGGAACT 
GCAGGGTCCT TATCCTCTAG CATTGTGGAA GCCAATGTCC CCATGGTCCA ACCTAATACA 
TCACCGGGCC CCACACAGGA AGAGTCAGGC GAGAACAGTG TGGATGGTGA GAGAAATGCG 
AAGAGCTGCC TGTCTCAACA GGGCAGAGAA GCTGGCTTGC TGGAACCCAA CACAGCTGCT 
TCCTCTTCAG ACCCCGTGGA TGTATCTAAG GAAGAGGGAA GCGATGAGGT GCTGACTCCG 
ACTAAACCCA GGACAACAGA AGACCTGTTT GCAGCCATTC ACAGATCTAA AAGGAAAGTC 
CTTGGCCGGA AAGACTCAGA AGACGATCAC ACTCGAAATC ACTCTCCCTC CCCGCCTGTG 
ACACCCACGA GTGCTGCCCC TAACCTGGCC TCTCCAAAGC AAGTAGGGTC TATCCAGAGA 
AGCATAAAAA AGAGCACTAC CAGCAGTGAC AACTTCAAAG CTCTGCTGCT AAAGAAGGGG 
AGTCGCTCGG ACACCAGCGC TCGCATGTCA GCGGCTGAGA TGCTCAAGTC TACGGACCCT 
CGCTTCCAGA GATCTAGGTC GGAGCCTTCG GCAGACAGCC CGGACAGCCC ATCCAGCTGC 
TCCCCCAACA AGAACAGGAG AGCTCAGGAA GAGTGGGCCA AGAACGAAGG CCTGATGCCT 
CGGAGTCTGT CCTTTTCGGG CCCTCGGTAC AGCCGCAGCA GGACGCCACC CTCTGCGGCC 
AGCAGCAGGT ACAGCATGCG GAACCGCATC CAGAGCAGCC CCATGACCGT CATCTCCGAG 
GGAGAGGGAG AACCTGCTGA GCCAGCGGAC AACAAAGCAC GCAGGGCCCT GGATGCTACC 
AGGGTGTGCT CTCTGGACAG ACTGACGGGT CAGGAGATGG ACCAGGCCAG CCTCCTCTGT 
AGCGAGGAGC CTGCCTCTGT GGATGGGATA GGAAGGGCTG AGGGCAACGG TCCCTCGGAG 
CAGTGTGGGG GTACGGAACA GAAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512753.1
CDS	2556..7265
Translation	MFCLKAVSNLDEESRWTVHYTAPWHQQENVFLPATRPPCVEDLHRQAKLNLKSVLRECDKLRQDGCRSSQYYSQGPTFAAGSSPCDDYQDEDTEADRKCSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQDDDGSAHSLYVPDHYSTLGRLDSYRSTGQCLETRDTSCQTEDVKVIPPSMRRIRAHKGVGVAAQMSHLSGSSGNMSVLSDSAGVVFPSRLSNDTGFHSLPRTGPRASTYSLEGRMGALGSTEDTDDTSPYQGGSLQGHENFAHLGGASSTGMLSRPKSQQLRFLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRTPGSSSYSKIKPRDRPTPRCSVKDDHQSPRHHWNEGHLIHSRALASSVPGATTLLSLHDSEVSLNAPANRENGSQAILYHCRNNPSFPDHPSDVDGKSECSYSGDRGCGSSEPWEYKTSSNGRASPLKPHLATPGCSTPTSNVSSCSLDQTSLKGDTRSLCSEDHDGYYTTTHEAGNLYTLSDGLGNPRHSMVNVFDGRAQRSQGDQAAHQDKILSRNISLKKAKKPPLPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGKGGSSPSQSPCSDFEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGVTPSQSDTSSVKSEYTDPWGYYIDYTSLQEDPGNPTGGCSANTEAATGNGPVRHIQEGSRVPVPQVPGCSVRPKIASPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSMSISSSSTSLSSNTSTEGSGTMKKLDTTLASALAPPPPPLPPLPSPCLADKSPFLPPPPPLADCSEGSPLPPSPMFPPPPPEALVPFCSPTDGCLSPSPTAVSPSLPRSLPPVPAPPPFLPSSEPPPAPPLDPKLMKENRPFFKNSSQSESSREALRRPANKEEGCRPPMPLITTEALQMVQLRPVRKNSGAGAVLFSEPSAQEQRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLPSCSDGAPGPGPSLRTTLLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFPGVPSPTRAEGEAVRSQEEKSSPASRAGSHATAPTPGSPALEPGTAGSLSSSIVEANVPMVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMDQASLLCSEEPASVDGIGRAEGNGPSEQCGGTEQKS

Target ORF information:

RefSeq Version	XM_006512690.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512690.3

ORF Insert Sequence:

ATGTTTTGCC TGAAAGCGGT TTCCAACTTG GACGAGGAGA GTCGATGGAC TGTCCACTAC 
ACGGCACCAT GGCATCAGCA AGAGAATGTG TTCCTTCCCG CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG 
CTGAGGCAAG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC 
GGCTCCAGCC CCTGCGACGA TTATCAAGAT GAAGACACAG AAGCAGACCG AAAGTGCTCT 
CTCTCTTCAT CTGAAGAAGA AAGATTTATC GGAATCAGGA GACCTAAAAC GCCAACCTCA 
GGTGACTTCT CCGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA 
CCAGAAGAGA AGACGCGGCA GCAAGCCCAG ACGGTCCAGG CTGACGTGGT TCCTATAAAC 
ATAACTGGGG AGAATTTCGA TCGTCAGGCC AGCCTTCGGC GGTCTCTAAT TTACACAGAC 
ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CAATTTCAGG AATCCCTGAC 
ATCATTCAGA AGGAGCTAGC ATCAGCCACT GGCCAAGATG ATGATGGTAG TGCCCATTCG 
CTGTACGTCC CAGACCACTA CTCTACGTTA GGAAGGCTTG ATAGTTATCG GTCCACCGGG 
CAATGCTTGG AAACCAGGGA CACCAGCTGT CAGACCGAAG ACGTGAAGGT CATCCCACCA 
TCAATGAGAA GGATCAGGGC TCACAAGGGG GTAGGCGTTG CTGCCCAGAT GAGCCACCTC 
TCAGGTTCCT CAGGCAACAT GTCTGTGCTG AGTGACTCAG CCGGTGTTGT GTTCCCATCT 
CGCCTCAGTA ACGATACTGG TTTCCACAGT CTTCCACGTA CCGGCCCGAG AGCAAGCACC 
TATTCACTGG AGGGAAGGAT GGGTGCCCTG GGCTCTACTG AAGATACGGA TGACACTTCT 
CCCTACCAGG GGGGTAGCTT ACAGGGGCAT GAAAACTTTG CGCATTTAGG AGGTGCGTCG 
AGTACTGGGA TGCTTTCGAG GCCCAAATCC CAGCAGTTGA GGTTTTTGGA GAGTCCAGCA 
TGTGTGGTTT CACCTCATGC AGCCTACTCT ACCAGTGTCA TCCCAAATGC CACGCTACTG 
TCCTCTTCAG AGGTTATTGT CATTCACACT GCTCAGAGTG CAGGACAGCT GGACAGTAGA 
ACACCCGGTT CATCTTCATA TTCAAAAATA AAACCCAGAG ACCGTCCCAC ACCCAGATGT 
TCTGTGAAAG ATGACCATCA GTCCCCCCGT CATCACTGGA ACGAGGGTCA TCTCATTCAT 
TCACGGGCTT TAGCCTCCTC TGTCCCTGGT GCCACCACGC TGTTATCCCT CCATGATTCA 
GAGGTCTCTC TAAATGCCCC AGCAAACAGG GAAAATGGAT CCCAAGCCAT ACTCTATCAT 
TGTAGAAACA ACCCAAGCTT TCCTGACCAC CCTTCAGATG TGGATGGCAA GAGCGAGTGT 
AGTTATTCGG GGGACAGGGG ATGTGGCAGT TCTGAGCCCT GGGAATATAA AACCTCCAGC 
AATGGGCGGG CATCCCCACT GAAGCCACAC CTGGCCACTC CTGGTTGCTC TACTCCCACA 
AGTAACGTGA GCAGCTGCAG TTTGGACCAA ACATCTCTCA AGGGTGATAC CAGGTCCCTG 
TGTTCAGAGG ACCATGATGG TTACTATACT ACCACTCACG AGGCTGGGAA CCTGTACACT 
CTCAGTGATG GCTTGGGGAA CCCCAGGCAC AGCATGGTCA ATGTTTTTGA TGGAAGGGCT 
CAGAGAAGCC AAGGGGATCA GGCCGCTCAC CAGGATAAAA TCCTTTCGAG AAACATCTCT 
CTGAAGAAAG CAAAGAAACC ACCCCTGCCA CCATCCCGGA CCGATTCTCT GCGAAGGATT 
CCCAAAAAGA ACAACCAGAC AAACGGTCAG GTGCTCAATG AGAGCCTGAT CGCCTCGCTC 
CAGCATTCAC TGCAGCTAAG CCTCCCTGGC AAGGGCGGCA GCTCCCCTTC CCAGAGCCCC 
TGCAGTGACT TTGAGGAACC CTGGCTCCCC AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG 
GGTAGTAGCT TGACCTCCAC CACCACTCCC AACGTGTATT CTCTGTGTGG GGTCACTCCG 
TCCCAGAGCG ACACAAGTAG CGTCAAGTCG GAATACACGG ACCCTTGGGG TTACTACATT 
GACTACACGA GCTTGCAGGA AGATCCAGGG AACCCCACCG GGGGCTGCTC AGCCAACACT 
GAGGCAGCAA CTGGAAACGG GCCAGTACGC CACATCCAGG AGGGGTCGAG AGTCCCAGTG 
CCCCAAGTGC CGGGCTGCTC AGTTAGACCA AAGATCGCCT CACCAGAGAA GTCACAGAGA 
GTCACCTCTC CATCCAGTGG GTATTCTAGC CAGTCGAATA CACCCACAGC ACTCACCCCT 
GTGCCTGTGT TTTTAAAATC AATGTCACCA GCAAATGGAA AGGGGAAGGC CAAGCCCAAG 
GTACCAGAAA GAAAATCGTC TCTGATCTCT TCTATGTCCA TCTCATCATC GTCCACTTCT 
CTCTCTTCTA ATACTTCTAC CGAAGGAAGT GGGACCATGA AGAAACTGGA TACAACCCTG 
GCCTCGGCCC TTGCCCCTCC CCCTCCTCCT CTTCCCCCTC TGCCTTCTCC GTGTCTGGCA 
GACAAGTCCC CTTTTCTTCC ACCACCTCCT CCTCTAGCAG ATTGCTCCGA GGGCTCTCCT 
CTGCCGCCCT CTCCCATGTT CCCCCCTCCA CCACCAGAAG CCCTTGTTCC CTTCTGCTCC 
CCCACCGACG GGTGCCTTTC TCCTTCTCCC ACAGCAGTTA GCCCCTCTCT TCCAAGATCC 
TTGCCTCCTG TGCCAGCACC TCCACCTTTC TTGCCCTCAT CAGAACCCCC GCCCGCTCCG 
CCTCTGGACC CCAAACTCAT GAAAGAAAAC AGGCCCTTTT TCAAAAACTC TAGCCAGTCC 
GAATCCTCTA GGGAGGCCCT CAGGCGGCCT GCCAACAAGG AGGAGGGCTG CCGACCCCCC 
ATGCCCCTGA TCACCACAGA AGCTTTGCAG ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC 
TCAGGTGCCG GGGCAGTCCT GTTTTCTGAA CCATCAGCTC AGGAACAACG AACTCCGACT 
GCTCCACAGT ATCACTTAAA GCCATCTGCT TTCCTCAAAT CCCGAAATAG CATAAATGAA 
ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG ACGAGCTCGC TTCCGATGCC TGCCAAGAGC 
CAGAGTCAGG GTGACCATGA CAGTGCAGTA GAGCGTGGCG GCCTTCCGAG CTGCAGCGAT 
GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG ACCACTCTGC TCCCAGACTC TTCACCCAGC 
AGGAAGCCAC CCCCCATCTC TAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG 
AGAGATTTCA CAGCGGAGCC CACAGAGAAC GGGAGCGAAG CTTTCCCAGG CGTGCCCAGC 
CCTACCAGGG CAGAGGGGGA AGCTGTGCGG AGCCAAGAGG AGAAATCCAG CCCAGCATCC 
CGAGCTGGCT CTCATGCTAC GGCCCCCACC CCCGGCAGTC CGGCTCTAGA ACCGGGAACT 
GCAGGGTCCT TATCCTCTAG CATTGTGGAA GCCAATGTCC CCATGGTCCA ACCTAATACA 
TCACCGGGCC CCACACAGGA AGAGTCAGGC GAGAACAGTG TGGATGGTGA GAGAAATGCG 
AAGAGCTGCC TGTCTCAACA GGGCAGAGAA GCTGGCTTGC TGGAACCCAA CACAGCTGCT 
TCCTCTTCAG ACCCCGTGGA TGTATCTAAG GAAGAGGGAA GCGATGAGGT GCTGACTCCG 
ACTAAACCCA GGACAACAGA AGACCTGTTT GCAGCCATTC ACAGATCTAA AAGGAAAGTC 
CTTGGCCGGA AAGACTCAGA AGACGATCAC ACTCGAAATC ACTCTCCCTC CCCGCCTGTG 
ACACCCACGA GTGCTGCCCC TAACCTGGCC TCTCCAAAGC AAGTAGGGTC TATCCAGAGA 
AGCATAAAAA AGAGCACTAC CAGCAGTGAC AACTTCAAAG CTCTGCTGCT AAAGAAGGGG 
AGTCGCTCGG ACACCAGCGC TCGCATGTCA GCGGCTGAGA TGCTCAAGTC TACGGACCCT 
CGCTTCCAGA GATCTAGGTC GGAGCCTTCG GCAGACAGCC CGGACAGCCC ATCCAGCTGC 
TCCCCCAACA AGAACAGGAG AGCTCAGGAA GAGTGGGCCA AGAACGAAGG CCTGATGCCT 
CGGAGTCTGT CCTTTTCGGG CCCTCGGTAC AGCCGCAGCA GGACGCCACC CTCTGCGGCC 
AGCAGCAGGT ACAGCATGCG GAACCGCATC CAGAGCAGCC CCATGACCGT CATCTCCGAG 
GGAGAGGGAG AACCTGCTGA GCCAGCGGAC AACAAAGCAC GCAGGGCCCT GGATGCTACC 
AGGGTGTGCT CTCTGGACAG ACTGACGGGT CAGGAGATGG ACCAGGCCAG CCTCCTCTGT 
AGCGAGGAGC CTGCCTCTGT GGATGGGATA GGAAGGGCTG AGGGCAACGG TCCCTCGGAG 
CAGTGTGGGG GTACGGAACA GAAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Nhsl1
Entrez Gene ID	215819
Full Name	NHS-like 1
Synonyms	5730409E15Rik,A630035H13Rik,A730096F01,BC013565,D10Bwg0940e,mKIAA1357
General protein information	Preferred Names NHS-like 1 Names NHS-like protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 A3\|10 7.65 cM

Pan troglodytes (chimpanzee)	NHSL1	XP_001171693.2
Mus musculus (house mouse)	Nhsl1	NP_775566.3
Danio rerio (zebrafish)	nhsl1b	XP_005160821.1
Canis lupus familiaris (dog)	NHSL1	XP_005615510.1
Rattus norvegicus (Norway rat)	Nhsl1	XP_006227729.1
	NHSL1	XP_001095123.2
Xenopus tropicalis (tropical clawed frog)	nhsl1	NP_001135597.1
Homo sapiens (human)	NHSL1	NP_065197.1
Bos taurus (cattle)	NHSL1	NP_001178467.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Nhsl1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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