Prss3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Prss3
Entrez Gene ID	22073
Full Name	protease, serine 3
Synonyms	MTG,TRY4,Tb,Try3
General protein information	Preferred Names protease, serine 3 Names mesotrypsin trypsin 3 trypsinogen 15
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 B1

mRNA and Protein(s)

mRNA Protein Name

NM_011645.2 NP_035775.1 mesotrypsin precursor
Pathways from BioSystems

mRNA	Protein	Name
NM_011645.2	NP_035775.1	mesotrypsin precursor

Homologies

Rattus norvegicus (Norway rat)	Try10	NP_001004097.1
Rattus norvegicus (Norway rat)	LOC683849	XP_003749780.1
Rattus norvegicus (Norway rat)	LOC102554637	XP_006236448.1
Mus musculus (house mouse)	Gm10334	NP_001096623.1
Rattus norvegicus (Norway rat)	Prss2	NP_036861.1
Mus musculus (house mouse)	Prss1	NP_444473.1
Mus musculus (house mouse)	Prss3	NP_035775.1
Rattus norvegicus (Norway rat)	LOC100912089	XP_003749781.1
Mus musculus (house mouse)	Gm5771	NP_001034086.1

Gene Ontology
Bibliography

Related articles in PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

Mesotrypsin and caspase-14 participate in prosaposin processing: potential relevance to epidermal permeability barrier formation.
Yamamoto-Tanaka M, Motoyama A, Miyai M, Matsunaga Y, Matsuda J, Tsuboi R, Hibino T
The Journal of biological chemistry289(29)20026-38(2014 Jul)

Intracellular activation of trypsinogen in transgenic mice induces acute but not chronic pancreatitis.
Gaiser S, Daniluk J, Liu Y, Tsou L, Chu J, Lee W, Longnecker DS, Logsdon CD, Ji B
Gut60(10)1379-88(2011 Oct)

Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells.
Sherwood MW, Prior IA, Voronina SG, Barrow SL, Woodsmith JD, Gerasimenko OV, Petersen OH, Tepikin AV
Proceedings of the National Academy of Sciences of the United States of America104(13)5674-9(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mesotrypsin generated saposins A-D from prosaposin, and mature caspase-14 contributed to this process by activating mesotrypsinogen to mesotrypsin. Knockdown of these proteases markedly down-regulated saposin A synthesis in skin equivalent models.
Title: Mesotrypsin and caspase-14 participate in prosaposin processing: potential relevance to epidermal permeability barrier formation.

Intra-acinar activation of trypsinogen is sufficient to initiate acute pancreatitis.
Title: Intracellular activation of trypsinogen in transgenic mice induces acute but not chronic pancreatitis.

the initiation of acute pancreatitis depends on endocytic vacuole formation and trypsinogen activation in pancreatic acinar cells
Title: Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells.

These results underscore the importance of trypsinogen PRSS1 mutations as pathogenic mediators of hereditary pancreatitis and indicate that persistent pancreatic injury might be causally linked to chronic pancreatitis.
Title: A mouse model of hereditary pancreatitis generated by transgenic expression of R122H trypsinogen.

The following Prss3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prss3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08516	NM_011645.2 Latest version!	Mus musculus protease, serine 3 (Prss3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prss3 ( NM_011645.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035775.1 Mus musculus protease, serine 3 (Prss3), mRNA.

Price & Availability↑

CloneID	OMu08516
Clone ID Related Accession (Same CDS sequence)	NM_011645.2
Accession Version	NM_011645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	mesotrypsin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158659.6. On Sep 7, 2007 this sequence version replaced NM_011645.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC098498.1, AK131862.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849376, SAMN00849377 [ECO:0000350] ##Evidence-Data-END##

ATGAATGCAC TCCTGATCCT GGCCCTTGTG GGAGCTGCTG TTGCCTTCCC CGTGGATGAT 
GATGACAAGA TCGTTGGAGG ATACACCTGC CAAGAGAATT CTGTCCCCTA CCAGGTGTCC 
CTGAACTCTG GCTACCACTT CTGTGGAGGC TCCCTCATCA ATGACCAGTG GGTGGTGTCT 
GCAGCTCACT GCTACAAAAC CCGCATCCAA GTGAGATTGG GGGAGCACAA CATCAACGTC 
CTGGAGGGCA ATGAGCAGTT TGTCAATGCT GCCAAGATCA TCAAGCACCC CAACTTCAAT 
AGGAAGACAC TGAACAACGA CATTATGTTG CTCAAGCTCT CTTCCCCTGT GACCCTCAAT 
GCCAGAGTGG CCACTGTGGC TCTGCCCAGC TCCTGTGCAC CTGCTGGCAC TCAGTGTCTC 
ATCTCTGGCT GGGGCAACAC TCTTAGCTTT GGTGTCAGTG AACCAGACCT GCTCCAGTGC 
CTGGATGCCC CACTGCTGCC TCAGGCTGAC TGTGAAGCCT CCTATCCTGG GAAGATCACT 
GGTAACATGG TCTGTGCTGG CTTCCTAGAG GGAGGCAAGG ATTCCTGCCA GGGTGACTCC 
GGTGGCCCTG TGGTCTGCAA TAGAGAACTC CAGGGTATAG TCTCCTGGGG CTATGGCTGT 
GCCCTGCCAG ACAATCCTGG TGTGTACACC AAGGTCTGCA ACTATGTGGA CTGGATTCAG 
GACACAATTG CTGCCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035775.1
CDS	17..757
Translation	MNALLILALVGAAVAFPVDDDDKIVGGYTCQENSVPYQVSLNSGYHFCGGSLINDQWVVSAAHCYKTRIQVRLGEHNINVLEGNEQFVNAAKIIKHPNFNRKTLNNDIMLLKLSSPVTLNARVATVALPSSCAPAGTQCLISGWGNTLSFGVSEPDLLQCLDAPLLPQADCEASYPGKITGNMVCAGFLEGGKDSCQGDSGGPVVCNRELQGIVSWGYGCALPDNPGVYTKVCNYVDWIQDTIAAN

Target ORF information:

RefSeq Version	NM_011645.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protease, serine 3 (Prss3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011645.2

ORF Insert Sequence:

ATGAATGCAC TCCTGATCCT GGCCCTTGTG GGAGCTGCTG TTGCCTTCCC CGTGGATGAT 
GATGACAAGA TCGTTGGAGG ATACACCTGC CAAGAGAATT CTGTCCCCTA CCAGGTGTCC 
CTGAACTCTG GCTACCACTT CTGTGGAGGC TCCCTCATCA ATGACCAGTG GGTGGTGTCT 
GCAGCTCACT GCTACAAAAC CCGCATCCAA GTGAGATTGG GGGAGCACAA CATCAACGTC 
CTGGAGGGCA ATGAGCAGTT TGTCAATGCT GCCAAGATCA TCAAGCACCC CAACTTCAAT 
AGGAAGACAC TGAACAACGA CATTATGTTG CTCAAGCTCT CTTCCCCTGT GACCCTCAAT 
GCCAGAGTGG CCACTGTGGC TCTGCCCAGC TCCTGTGCAC CTGCTGGCAC TCAGTGTCTC 
ATCTCTGGCT GGGGCAACAC TCTTAGCTTT GGTGTCAGTG AACCAGACCT GCTCCAGTGC 
CTGGATGCCC CACTGCTGCC TCAGGCTGAC TGTGAAGCCT CCTATCCTGG GAAGATCACT 
GGTAACATGG TCTGTGCTGG CTTCCTAGAG GGAGGCAAGG ATTCCTGCCA GGGTGACTCC 
GGTGGCCCTG TGGTCTGCAA TAGAGAACTC CAGGGTATAG TCTCCTGGGG CTATGGCTGT 
GCCCTGCCAG ACAATCCTGG TGTGTACACC AAGGTCTGCA ACTATGTGGA CTGGATTCAG 
GACACAATTG CTGCCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

Mesotrypsin and caspase-14 participate in prosaposin processing: potential relevance to epidermal permeability barrier formation.
The Journal of biological chemistry289(29)20026-38(2014 Jul)
Yamamoto-Tanaka M,Motoyama A,Miyai M,Matsunaga Y,Matsuda J,Tsuboi R,Hibino T

Intracellular activation of trypsinogen in transgenic mice induces acute but not chronic pancreatitis.
Gut60(10)1379-88(2011 Oct)
Gaiser S,Daniluk J,Liu Y,Tsou L,Chu J,Lee W,Longnecker DS,Logsdon CD,Ji B

Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells.
Proceedings of the National Academy of Sciences of the United States of America104(13)5674-9(2007 Mar)
Sherwood MW,Prior IA,Voronina SG,Barrow SL,Woodsmith JD,Gerasimenko OV,Petersen OH,Tepikin AV

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Prss3 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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