Mogat2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mogat2
Entrez Gene ID	233549
Full Name	monoacylglycerol O-acyltransferase 2
Synonyms	DGAT2L5,MGAT2,Mgat1l
General protein information	Preferred Names monoacylglycerol O-acyltransferase 2 Names 2-acylglycerol O-acyltransferase 2 acyl CoA:monoacylglycerol acyltransferase 2 diacylglycerol acyltransferase 2-like protein 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_177448.4	NP_803231.1	2-acylglycerol O-acyltransferase 2
XM_011241737.1	XP_011240039.1	2-acylglycerol O-acyltransferase 2 isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG1946	NP_610319.2
Pan troglodytes (chimpanzee)	MOGAT2	XP_522112.2
Rattus norvegicus (Norway rat)	Mogat2	NP_001102906.2
Danio rerio (zebrafish)	mogat2	NP_001006083.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005949	XP_315985.3
Caenorhabditis elegans (roundworm)	Y53G8B.2	NP_497659.1
	MOGAT2	XP_001086603.1
Bos taurus (cattle)	MOGAT2	NP_001092606.1
Caenorhabditis elegans (roundworm)	K07B1.4	NP_505413.1
Homo sapiens (human)	MOGAT2	NP_079374.2
Canis lupus familiaris (dog)	MOGAT2	XP_542304.1
Mus musculus (house mouse)	Mogat2	NP_803231.1
Caenorhabditis elegans (roundworm)	dgtr-1	NP_504649.1
Caenorhabditis elegans (roundworm)	CELE_F59A1.10	NP_507469.1
Gallus gallus (chicken)	MOGAT2	XP_004939048.1
Drosophila melanogaster (fruit fly)	CG1942	NP_610318.1
Drosophila melanogaster (fruit fly)	CG1941	NP_610317.1
Xenopus tropicalis (tropical clawed frog)	mogat1	XP_002941212.2

Gene Ontology
Bibliography

Related articles in PubMed

Diacylglycerol acyltransferase-2 and monoacylglycerol acyltransferase-2 are ubiquitinated proteins that are degraded by the 26S proteasome.
Brandt C, McFie PJ, Stone SJ
The Biochemical journal473(20)3621-3637(2016 10)

Membrane topology of human monoacylglycerol acyltransferase-2 and identification of regions important for its localization to the endoplasmic reticulum.
McFie PJ, Izzard S, Vu H, Jin Y, Beauchamp E, Berthiaume LG, Stone SJ
Biochimica et biophysica acta1861(9 Pt A)1192-1204(2016 09)

Adult-onset deficiency of acyl CoA:monoacylglycerol acyltransferase 2 protects mice from diet-induced obesity and glucose intolerance.
Banh T, Nelson DW, Gao Y, Huang TN, Yen MI, Yen CL
Journal of lipid research56(2)379-89(2015 Feb)

MGAT2 deficiency and vertical sleeve gastrectomy have independent metabolic effects in the mouse.
Mul JD, Begg DP, Haller AM, Pressler JW, Sorrell J, Woods SC, Farese RV Jr, Seeley RJ, Sandoval DA
American journal of physiology. Endocrinology and metabolism307(11)E1065-72(2014 Dec)

Intestine-specific deletion of acyl-CoA:monoacylglycerol acyltransferase (MGAT) 2 protects mice from diet-induced obesity and glucose intolerance.
Nelson DW, Gao Y, Yen MI, Yen CL
The Journal of biological chemistry289(25)17338-49(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MGAT2 in the intestine plays an indispensable role in enhancing metabolic efficiency but also raise the possibility that MGAT2 in other tissues may contribute to the regulation of energy metabolism.
Title: Intestine-specific expression of MOGAT2 partially restores metabolic efficiency in Mogat2-deficient mice.

the release of gut peptides following oral triglyceride ingestion in MGAT2 and DGAT1 knock-out mice was investigated.
Title: Role of MGAT2 and DGAT1 in the release of gut peptides after triglyceride ingestion.

Mogat2 is a key determinant of energy metabolism in response to dietary fat and suggest that inhibition or deletion of this enzyme may prove to be a useful strategy for treating obesity.
Title: Deficiency of the intestinal enzyme acyl CoA:monoacylglycerol acyltransferase-2 protects mice from metabolic disorders induced by high-fat feeding.

MGAT2 possessed an intrinsic acyl-CoA:diacylglycerol acyltransferase (DGAT) activity which could provide an alternative pathway for triacylglycerol synthesis in the absence of DGAT
Title: Properties of the mouse intestinal acyl-CoA:monoacylglycerol acyltransferase, MGAT2.

MGAT catalyzes the synthesis of diacylglycerol, a precursor of triacylglycerol. MGAT mediates the absorption of dietary fat by catalyzing the resynthesis of triacylglycerol in enterocytes, a step required for delivering absorbed fat to other tissues.
Title: MGAT2, a monoacylglycerol acyltransferase expressed in the small intestine.

The following Mogat2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mogat2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06409	NM_177448.4 Latest version!	Mus musculus monoacylglycerol O-acyltransferase 2 (Mogat2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu73685	XM_011241737.1 Latest version!	Mus musculus monoacylglycerol O-acyltransferase 2 (Mogat2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mogat2 ( NM_177448.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_803231.1 Mus musculus monoacylglycerol O-acyltransferase 2 (Mogat2), mRNA.

Price & Availability↑

CloneID	OMu06409
Clone ID Related Accession (Same CDS sequence)	NM_177448.4
Accession Version	NM_177448.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Mus musculus(house mouse)
Product	2-acylglycerol O-acyltransferase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK133854.1 and BC052831.1. On Jun 18, 2008 this sequence version replaced NM_177448.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052831.1, AK133854.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGAGT TCGCCCCCCT GTTGGTACCA TGGGAGCGCA GGTTACAGAC CTTCGCGGTC 
CTTCAGTGGG TCTTCTCCTT CCTGGCCTTG GCCCAGCTCT GCATCGTCAT CTTCGTAGGC 
CTCCTATTCA CAAGGTTCTG GCTCTTCTCT GTCCTGTATG CCACCTGGTG GTACCTGGAC 
TGGGACAAGC CGCGGCAGGG AGGCCGGCCC ATCCAGTTCT TCAGACGCTT GGCCATATGG 
AAGTACATGA AGGATTATTT CCCTGTCTCT TTGGTCAAGA CAGCTGAGCT GGACCCTTCC 
CGGAACTACA TCGCGGGCTT CCACCCCCAT GGAGTCCTAG CAGCTGGAGC CTTTCTTAAC 
CTGTGCACTG AAAGCACGGG CTTTACCTCG CTTTTCCCGG GCATCCGCTC CTATCTGATG 
ATGCTGACTG TGTGGTTCCG GGCCCCCTTC TTCCGAGATT ACATCATGTC TGGGGGGCTG 
GTCTCATCAG AAAAGGTGAG TGCCGATCAC ATTCTGTCCA GGAAGGGCGG CGGGAACTTG 
CTTGCCATCA TCGTTGGGGG CGCGCAGGAG GCACTGGACG CCAGGCCTGG AGCCTACAGG 
CTGCTGCTGA AGAATCGCAA GGGCTTCATC AGGCTCGCCC TGATGCATGG GGCAGCTCTT 
GTGCCAATCT TCTCCTTTGG AGAAAACAAC CTGTTCAACC AGGTTGAGAA CACCCCTGGT 
ACCTGGCTGC GCTGGATCCA GAACCGGCTA CAGAAGATCA TGGGCATCTC CCTCCCTCTC 
TTCCACGGCA GAGGTGTCTT CCAGTACAGC TTTGGCCTCA TGCCCTTCCG CCAGCCCATC 
ACCACCATAG TGGGGAAGCC CATCGAGGTG CAGATGACAC CACAGCCCTC AAGGGAGGAG 
GTGGACCGGC TTCATCAGCG CTATATCAAG GAGCTCTGCA AGCTCTTTGA GGAGCACAAA 
CTCAAGTTCA ACGTCCCTGA GGACCAGCAT CTGGAGTTCT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_803231.1
CDS	47..1051
Translation	MVEFAPLLVPWERRLQTFAVLQWVFSFLALAQLCIVIFVGLLFTRFWLFSVLYATWWYLDWDKPRQGGRPIQFFRRLAIWKYMKDYFPVSLVKTAELDPSRNYIAGFHPHGVLAAGAFLNLCTESTGFTSLFPGIRSYLMMLTVWFRAPFFRDYIMSGGLVSSEKVSADHILSRKGGGNLLAIIVGGAQEALDARPGAYRLLLKNRKGFIRLALMHGAALVPIFSFGENNLFNQVENTPGTWLRWIQNRLQKIMGISLPLFHGRGVFQYSFGLMPFRQPITTIVGKPIEVQMTPQPSREEVDRLHQRYIKELCKLFEEHKLKFNVPEDQHLEFC

Target ORF information:

RefSeq Version	NM_177448.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus monoacylglycerol O-acyltransferase 2 (Mogat2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177448.4

ORF Insert Sequence:

ATGGTGGAGT TCGCCCCCCT GTTGGTACCA TGGGAGCGCA GGTTACAGAC CTTCGCGGTC 
CTTCAGTGGG TCTTCTCCTT CCTGGCCTTG GCCCAGCTCT GCATCGTCAT CTTCGTAGGC 
CTCCTATTCA CAAGGTTCTG GCTCTTCTCT GTCCTGTATG CCACCTGGTG GTACCTGGAC 
TGGGACAAGC CGCGGCAGGG AGGCCGGCCC ATCCAGTTCT TCAGACGCTT GGCCATATGG 
AAGTACATGA AGGATTATTT CCCTGTCTCT TTGGTCAAGA CAGCTGAGCT GGACCCTTCC 
CGGAACTACA TCGCGGGCTT CCACCCCCAT GGAGTCCTAG CAGCTGGAGC CTTTCTTAAC 
CTGTGCACTG AAAGCACGGG CTTTACCTCG CTTTTCCCGG GCATCCGCTC CTATCTGATG 
ATGCTGACTG TGTGGTTCCG GGCCCCCTTC TTCCGAGATT ACATCATGTC TGGGGGGCTG 
GTCTCATCAG AAAAGGTGAG TGCCGATCAC ATTCTGTCCA GGAAGGGCGG CGGGAACTTG 
CTTGCCATCA TCGTTGGGGG CGCGCAGGAG GCACTGGACG CCAGGCCTGG AGCCTACAGG 
CTGCTGCTGA AGAATCGCAA GGGCTTCATC AGGCTCGCCC TGATGCATGG GGCAGCTCTT 
GTGCCAATCT TCTCCTTTGG AGAAAACAAC CTGTTCAACC AGGTTGAGAA CACCCCTGGT 
ACCTGGCTGC GCTGGATCCA GAACCGGCTA CAGAAGATCA TGGGCATCTC CCTCCCTCTC 
TTCCACGGCA GAGGTGTCTT CCAGTACAGC TTTGGCCTCA TGCCCTTCCG CCAGCCCATC 
ACCACCATAG TGGGGAAGCC CATCGAGGTG CAGATGACAC CACAGCCCTC AAGGGAGGAG 
GTGGACCGGC TTCATCAGCG CTATATCAAG GAGCTCTGCA AGCTCTTTGA GGAGCACAAA 
CTCAAGTTCA ACGTCCCTGA GGACCAGCAT CTGGAGTTCT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mogat2 ( XM_011241737.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240039.1 Mus musculus monoacylglycerol O-acyltransferase 2 (Mogat2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73685
Clone ID Related Accession (Same CDS sequence)	XM_011241737.1
Accession Version	XM_011241737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	2-acylglycerol O-acyltransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGCA GATCTGACCT GGCTCCTGAG GTGTGGCTCT GTCCCTCTCC ACCCTGTAGA 
TGCTCCAGCA CCGCTGTGGA CCGGGAAGGA AGAGTGGCTG GGAGCACGGG AGGGAGCCTT 
ATCTCCTTTG TTGCCCAGCT CTGCATCGTC ATCTTCGTAG GCCTCCTATT CACAAGGTTC 
TGGCTCTTCT CTGTCCTGTA TGCCACCTGG TGGTACCTGG ACTGGGACAA GCCGCGGCAG 
GGAGGCCGGC CCATCCAGTT CTTCAGACGC TTGGCCATAT GGAAGTACAT GAAGGATTAT 
TTCCCTGTCT CTTTGGTCAA GACAGCTGAG CTGGACCCTT CCCGGAACTA CATCGCGGGC 
TTCCACCCCC ATGGAGTCCT AGCAGCTGGA GCCTTTCTTA ACCTGTGCAC TGAAAGCACG 
GGCTTTACCT CGCTTTTCCC GGGCATCCGC TCCTATCTGA TGATGCTGAC TGTGTGGTTC 
CGGGCCCCCT TCTTCCGAGA TTACATCATG TCTGGGGGGC TGGTCTCATC AGAAAAGGTG 
AGTGCCGATC ACATTCTGTC CAGGAAGGGC GGCGGGAACT TGCTTGCCAT CATCGTTGGG 
GGCGCGCAGG AGGCACTGGA CGCCAGGCCT GGAGCCTACA GGCTGCTGCT GAAGAATCGC 
AAGGGCTTCA TCAGGCTCGC CCTGATGCAT GGGGCAGCTC TTGTGCCAAT CTTCTCCTTT 
GGAGAAAACA ACCTGTTCAA CCAGGTTGAG AACACCCCTG GTACCTGGCT GCGCTGGATC 
CAGAACCGGC TACAGAAGAT CATGGGCATC TCCCTCCCTC TCTTCCACGG CAGAGGTGTC 
TTCCAGTACA GCTTTGGCCT CATGCCCTTC CGCCAGCCCA TCACCACCAT AGTGGGGAAG 
CCCATCGAGG TGCAGATGAC ACCACAGCCC TCAAGGGAGG AGGTGGACCG GCTTCATCAG 
CGCTATATCA AGGAGCTCTG CAAGCTCTTT GAGGAGCACA AACTCAAGTT CAACGTCCCT 
GAGGACCAGC ATCTGGAGTT CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240039.1
CDS	16..1062
Translation	MKGRSDLAPEVWLCPSPPCRCSSTAVDREGRVAGSTGGSLISFVAQLCIVIFVGLLFTRFWLFSVLYATWWYLDWDKPRQGGRPIQFFRRLAIWKYMKDYFPVSLVKTAELDPSRNYIAGFHPHGVLAAGAFLNLCTESTGFTSLFPGIRSYLMMLTVWFRAPFFRDYIMSGGLVSSEKVSADHILSRKGGGNLLAIIVGGAQEALDARPGAYRLLLKNRKGFIRLALMHGAALVPIFSFGENNLFNQVENTPGTWLRWIQNRLQKIMGISLPLFHGRGVFQYSFGLMPFRQPITTIVGKPIEVQMTPQPSREEVDRLHQRYIKELCKLFEEHKLKFNVPEDQHLEFC

Target ORF information:

RefSeq Version	XM_011241737.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus monoacylglycerol O-acyltransferase 2 (Mogat2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241737.1

ORF Insert Sequence:

ATGAAAGGCA GATCTGACCT GGCTCCTGAG GTGTGGCTCT GTCCCTCTCC ACCCTGTAGA 
TGCTCCAGCA CCGCTGTGGA CCGGGAAGGA AGAGTGGCTG GGAGCACGGG AGGGAGCCTT 
ATCTCCTTTG TTGCCCAGCT CTGCATCGTC ATCTTCGTAG GCCTCCTATT CACAAGGTTC 
TGGCTCTTCT CTGTCCTGTA TGCCACCTGG TGGTACCTGG ACTGGGACAA GCCGCGGCAG 
GGAGGCCGGC CCATCCAGTT CTTCAGACGC TTGGCCATAT GGAAGTACAT GAAGGATTAT 
TTCCCTGTCT CTTTGGTCAA GACAGCTGAG CTGGACCCTT CCCGGAACTA CATCGCGGGC 
TTCCACCCCC ATGGAGTCCT AGCAGCTGGA GCCTTTCTTA ACCTGTGCAC TGAAAGCACG 
GGCTTTACCT CGCTTTTCCC GGGCATCCGC TCCTATCTGA TGATGCTGAC TGTGTGGTTC 
CGGGCCCCCT TCTTCCGAGA TTACATCATG TCTGGGGGGC TGGTCTCATC AGAAAAGGTG 
AGTGCCGATC ACATTCTGTC CAGGAAGGGC GGCGGGAACT TGCTTGCCAT CATCGTTGGG 
GGCGCGCAGG AGGCACTGGA CGCCAGGCCT GGAGCCTACA GGCTGCTGCT GAAGAATCGC 
AAGGGCTTCA TCAGGCTCGC CCTGATGCAT GGGGCAGCTC TTGTGCCAAT CTTCTCCTTT 
GGAGAAAACA ACCTGTTCAA CCAGGTTGAG AACACCCCTG GTACCTGGCT GCGCTGGATC 
CAGAACCGGC TACAGAAGAT CATGGGCATC TCCCTCCCTC TCTTCCACGG CAGAGGTGTC 
TTCCAGTACA GCTTTGGCCT CATGCCCTTC CGCCAGCCCA TCACCACCAT AGTGGGGAAG 
CCCATCGAGG TGCAGATGAC ACCACAGCCC TCAAGGGAGG AGGTGGACCG GCTTCATCAG 
CGCTATATCA AGGAGCTCTG CAAGCTCTTT GAGGAGCACA AACTCAAGTT CAACGTCCCT 
GAGGACCAGC ATCTGGAGTT CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diacylglycerol acyltransferase-2 and monoacylglycerol acyltransferase-2 are ubiquitinated proteins that are degraded by the 26S proteasome.
The Biochemical journal473(20)3621-3637(2016 10)
Brandt C,McFie PJ,Stone SJ

Membrane topology of human monoacylglycerol acyltransferase-2 and identification of regions important for its localization to the endoplasmic reticulum.
Biochimica et biophysica acta1861(9 Pt A)1192-1204(2016 09)
McFie PJ,Izzard S,Vu H,Jin Y,Beauchamp E,Berthiaume LG,Stone SJ

Adult-onset deficiency of acyl CoA:monoacylglycerol acyltransferase 2 protects mice from diet-induced obesity and glucose intolerance.
Journal of lipid research56(2)379-89(2015 Feb)
Banh T,Nelson DW,Gao Y,Huang TN,Yen MI,Yen CL

MGAT2 deficiency and vertical sleeve gastrectomy have independent metabolic effects in the mouse.
American journal of physiology. Endocrinology and metabolism307(11)E1065-72(2014 Dec)
Mul JD,Begg DP,Haller AM,Pressler JW,Sorrell J,Woods SC,Farese RV Jr,Seeley RJ,Sandoval DA

Intestine-specific deletion of acyl-CoA:monoacylglycerol acyltransferase (MGAT) 2 protects mice from diet-induced obesity and glucose intolerance.
The Journal of biological chemistry289(25)17338-49(2014 Jun)
Nelson DW,Gao Y,Yen MI,Yen CL

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Mogat2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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