Kcnk18 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnk18
Entrez Gene ID	332396
Full Name	potassium channel, subfamily K, member 18
Synonyms	Gm781,Tresk,Tresk-2,Trik
General protein information	Preferred Names potassium channel, subfamily K, member 18 Names potassium channel subfamily K member 18 2P K ion channel TRESK TWIK-related individual K+ channel TWIK-related spinal cord K+ channel two-pore-domain potassium channel TRESK
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_207261.3	NP_997144.1	potassium channel subfamily K member 18

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Kcnk18	NP_001003820.1
Danio rerio (zebrafish)	kcnk18	NP_001177236.2
Homo sapiens (human)	KCNK18	NP_862823.1
Pan troglodytes (chimpanzee)	KCNK18	XP_521614.2
Mus musculus (house mouse)	Kcnk18	NP_997144.1
Gallus gallus (chicken)	KCNK18	XP_001234729.2
Xenopus tropicalis (tropical clawed frog)	LOC100486306	XP_002943434.2
	KCNK18	XP_001095493.1
Canis lupus familiaris (dog)	KCNK18	XP_003640047.1
Bos taurus (cattle)	KCNK18	XP_002698581.1

Gene Ontology
Bibliography

Related articles in PubMed

Pyrethroids inhibit K2P channels and activate sensory neurons: basis of insecticide-induced paraesthesias.
Castellanos A, Andres A, Bernal L, Callejo G, Comes N, Gual A, Giblin JP, Roza C, Gasull X
Pain(2017 Sep)

Large-scale analysis of ion channel gene expression in the mouse heart during perinatal development.
Harrell MD, Harbi S, Hoffman JF, Zavadil J, Coetzee WA
Physiological genomics28(3)273-83(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Migraine-associated TRESK mutation, but not the C110R variant, reduces the endogenous TRESK currents to a degree that affects trigeminal ganglion neuron excitability
Title: Nonmigraine-associated TRESK K+ channel variant C110R does not increase the excitability of trigeminal ganglion neurons.

Identification of blocker binding site in mouse TRESK by molecular modeling and mutational studies
Title: Identification of blocker binding site in mouse TRESK by molecular modeling and mutational studies.

a role for TRESK in the pathogenesis of typical migraine with aura.
Title: A dominant-negative mutation in the TRESK potassium channel is linked to familial migraine with aura.

single residue of TRESK was found to be glycosylated upon heterologous expression. Signals of the N-glycosylation mutants were reduced by >50% because of inadequate surface expression of the channel.
Title: N-linked glycosylation determines cell surface expression of two-pore-domain K+ channel TRESK.

Because 14-3-3 proteins are ubiquitous, they are expected to control the duration of calcineurin-mediated TRESK activation in all the cell types that express the channel, depending on the phosphorylation state of serine 264.
Title: Phosphorylation-dependent binding of 14-3-3 proteins controls TRESK regulation.

The following Kcnk18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnk18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu09922	NM_207261.3 Latest version!	Mus musculus potassium channel, subfamily K, member 18 (Kcnk18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnk18 ( NM_207261.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_997144.1 Mus musculus potassium channel, subfamily K, member 18 (Kcnk18), mRNA.

Price & Availability↑

CloneID	OMu09922
Clone ID Related Accession (Same CDS sequence)	NM_207261.3
Accession Version	NM_207261.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily K member 18
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107710.5. On Nov 17, 2006 this sequence version replaced NM_207261.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK139600.1, AK162136.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCTG AGGAGCCACC TGAGGCCAGG AGATGCTGTC CCGAGGCCCT GGGGAAGGCC 
AGGGGATGCT GCCCCGAAGC CCTGGGCAAG CTTCTGCCCG GCCTCTGCTT CCTTTGCTGC 
CTGGTGACCT ATGCGCTGGT GGGTGCTGCT CTCTTCTCCG CTGTCGAGGG CCGCCCTGAC 
CCAGAAGCAG AGGAGAACCC TGAGTTGAAG AAGTTCCTGG ACGATCTGTG CAACATCCTG 
AAATGTAACC TGACAGTGGT TGAAGGTAGC AGGAAGAACT TGTGTGAGCA TCTGCAACAC 
CTCAAGCCCC AGTGGCTCAA GGCGCCCCAG GACTGGTCCT TCCTGAGCGC TCTCTTCTTC 
TGCTGCACAG TGTTCAGCAC AGTGGGTTAT GGCCACATGT ACCCTGTCAC CAGGCTCGGT 
AAGTTCCTGT GCATGCTGTA TGCGCTCTTT GGAATCCCTC TAATGTTCCT GGTCCTCACA 
GACATAGGAG ATATCCTGGC CACCATCTTA TCCAGGGCTT ACAGTCGGTT CCAGGCTCTC 
CTTTGCCTCC CCCACGATAT CTTCAAATGG CGCTCCCTCC CGCTCTGCCG GAAGCAGCCT 
GACAGCAAAC CGGTGGAGGA AGCCATCCCT CAGATTGTCA TTGATGCTGG TGTGGATGAA 
CTCCTAAACC CGCAGCCCAG CAAGGACCCC CCCTCTCCGA GCTGCAATGT GGAGCTGTTT 
GAGAGATTAG TTGCCCGTGA GAAAAAGAAC AAGCTACAAC CACCCACGCG TCCCGTGGAG 
AGGAGCAACT CCTGTCCCGA GCTGGTGCTG GGGCGACTGT CCTGTTCTAT TCTCAGCAAT 
CTGGATGAAG TGGGCCAGCA GGTGGAGAGG CTGGACATCC CTCTCCCCGT CATCGCCCTG 
GTCGTCTTTG CATACATCTC CTGCGCGGCT GCTATCCTCC CCTTCTGGGA GACCGAGCTA 
GGCTTCGAGG ATGCTTTCTA CTTCTGCTTT GTGACACTGA CCACCATCGG GTTTGGGGAC 
ATCGTGCTGG TGCACCCTCA TTTCTTCCTC TTCTTCTCCA TCTACATCAT CGTGGGCATG 
GAGATCCTGT TCATTGCCTT CAAGCTGATG CAGAACCGGC TCCTGCACAC CTACAAAACC 
CTCATGCTGT TTGTTTGCCA AAGGGAAGTT TCGCTACCTT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997144.1
CDS	87..1271
Translation	MEAEEPPEARRCCPEALGKARGCCPEALGKLLPGLCFLCCLVTYALVGAALFSAVEGRPDPEAEENPELKKFLDDLCNILKCNLTVVEGSRKNLCEHLQHLKPQWLKAPQDWSFLSALFFCCTVFSTVGYGHMYPVTRLGKFLCMLYALFGIPLMFLVLTDIGDILATILSRAYSRFQALLCLPHDIFKWRSLPLCRKQPDSKPVEEAIPQIVIDAGVDELLNPQPSKDPPSPSCNVELFERLVAREKKNKLQPPTRPVERSNSCPELVLGRLSCSILSNLDEVGQQVERLDIPLPVIALVVFAYISCAAAILPFWETELGFEDAFYFCFVTLTTIGFGDIVLVHPHFFLFFSIYIIVGMEILFIAFKLMQNRLLHTYKTLMLFVCQREVSLPW

Target ORF information:

RefSeq Version	NM_207261.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily K, member 18 (Kcnk18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207261.3

ORF Insert Sequence:

ATGGAGGCTG AGGAGCCACC TGAGGCCAGG AGATGCTGTC CCGAGGCCCT GGGGAAGGCC 
AGGGGATGCT GCCCCGAAGC CCTGGGCAAG CTTCTGCCCG GCCTCTGCTT CCTTTGCTGC 
CTGGTGACCT ATGCGCTGGT GGGTGCTGCT CTCTTCTCCG CTGTCGAGGG CCGCCCTGAC 
CCAGAAGCAG AGGAGAACCC TGAGTTGAAG AAGTTCCTGG ACGATCTGTG CAACATCCTG 
AAATGTAACC TGACAGTGGT TGAAGGTAGC AGGAAGAACT TGTGTGAGCA TCTGCAACAC 
CTCAAGCCCC AGTGGCTCAA GGCGCCCCAG GACTGGTCCT TCCTGAGCGC TCTCTTCTTC 
TGCTGCACAG TGTTCAGCAC AGTGGGTTAT GGCCACATGT ACCCTGTCAC CAGGCTCGGT 
AAGTTCCTGT GCATGCTGTA TGCGCTCTTT GGAATCCCTC TAATGTTCCT GGTCCTCACA 
GACATAGGAG ATATCCTGGC CACCATCTTA TCCAGGGCTT ACAGTCGGTT CCAGGCTCTC 
CTTTGCCTCC CCCACGATAT CTTCAAATGG CGCTCCCTCC CGCTCTGCCG GAAGCAGCCT 
GACAGCAAAC CGGTGGAGGA AGCCATCCCT CAGATTGTCA TTGATGCTGG TGTGGATGAA 
CTCCTAAACC CGCAGCCCAG CAAGGACCCC CCCTCTCCGA GCTGCAATGT GGAGCTGTTT 
GAGAGATTAG TTGCCCGTGA GAAAAAGAAC AAGCTACAAC CACCCACGCG TCCCGTGGAG 
AGGAGCAACT CCTGTCCCGA GCTGGTGCTG GGGCGACTGT CCTGTTCTAT TCTCAGCAAT 
CTGGATGAAG TGGGCCAGCA GGTGGAGAGG CTGGACATCC CTCTCCCCGT CATCGCCCTG 
GTCGTCTTTG CATACATCTC CTGCGCGGCT GCTATCCTCC CCTTCTGGGA GACCGAGCTA 
GGCTTCGAGG ATGCTTTCTA CTTCTGCTTT GTGACACTGA CCACCATCGG GTTTGGGGAC 
ATCGTGCTGG TGCACCCTCA TTTCTTCCTC TTCTTCTCCA TCTACATCAT CGTGGGCATG 
GAGATCCTGT TCATTGCCTT CAAGCTGATG CAGAACCGGC TCCTGCACAC CTACAAAACC 
CTCATGCTGT TTGTTTGCCA AAGGGAAGTT TCGCTACCTT GGTAA