Nphs1 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Nphs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nphs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu20792 NM_019459.2
Latest version!
Mus musculus nephrosis 1, nephrin (Nphs1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $741.30
$1059.00
OMu69564 XM_011250647.2
Latest version!
Mus musculus nephrosis 1, nephrin (Nphs1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OMu69564 XM_011250647.1 Mus musculus nephrosis 1, nephrin (Nphs1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu20792
    Clone ID Related Accession (Same CDS sequence) NM_019459.2
    Accession Version NM_019459.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3771bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Mus musculus(house mouse)
    Product nephrin precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF191090.1, AF172256.1, AK141081.1 and AC167970.4. On Jan 4, 2007 this sequence version replaced NM_019459.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF172256.1, AF191090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN00849388 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGAGCTA AGGAAGCCAC AGTCAGAGGC CCCGGGGCAA GCCCAGTGCA CAGAACTTGC 
    CACCTGATTC CCCTGCTGCT CGCGGGAATG CTGACCACAG GCCTGGCCCA GTCGCCAGTC
    CCCACCTCAG CACCTCGAGG CTTCTGGGCT CTATCTGAAA ACCTGACTGT GGTGGAAGGG
    TCGACAGTTA AGCTGTGGTG TGGCGTCAGG GCCCCCGGCA GTGTGGTGCA GTGGGCTAAG
    GATGGGCTGC TTCTGGGTCC AAACCCTAAG ATTCCAGGCT TCCCAAGGTA CAGCCTGGAA
    GGAGACAGTG CTAAAGGTGA GTTCCACCTG CTTATTGAAG CCTGTGACCT CAGCGATGAT
    GCGGAGTACG AGTGCCAAGT CGGCCGCTCC GAGTTGGGTC CCGAGCTCGT GTCTCCCAGC
    GTAATCCTCT CCATCCTAGT TTCCCCCAAG GTGCTTCAGT TAACCCCTGA GGCAGGAAGC
    ACAGTTACCT GGGTAGCTGG ACAGGAATAT GTGGTCACCT GTGTGTCTGG GGATGCAAAG
    CCAGCACCTG ACATCATCTT CATCCAGGGT GGACGCACGG TAGAGGATGT CTCCTCCAGC
    GTGAATGAGG GATCAGAGGA GAAACTCTTC TTCACGGAAG CCGAAGCCAG GGTGACACCC
    CAGAGCTCAG ATAATGGGCA GCTGCTAGTC TGCGAGGGGT CCAACCCAGC CTTGGCCACT
    CCCATAAAGG CTTCGTTCAC CATGAATATC CTGTTCCCCC CAGGACCTCC TGTCATTGAT
    TGGCCAGGCC TGAATGAGGG GCATGTACGG GCAGGGGAGA ACCTGGAGCT ACCCTGCATA
    GCCAGAGGTG GAAATCCACC TGCGACCCTG CAGTGGCTGA AGAATGGTAA ACCAGTGTCC
    ATAGCTTGGG GCACAGAGCA TGCCCAGGCA GTGGCTCACA GTGTGCTGGT GATGACCGTT
    CGACCTGAAG ACCACGGAGC TCGGCTCAGC TGTCAGTCCT ACAACAGTGT GTCTGCAGAG
    ACCCAGGAGC GAAGCATCAC TCTGCAGGTC ACCTTTCCCC CCAGTGCCGT TACCATCCTG
    GGATCTACAT CACAGTCTGA GAACAAAAAT GTGACCCTTT GCTGCCTTAC CAAGTCCAGT
    CGCCCACGGG TCCTGCTGCG ATGGTGGTTG GGTGGACGGC AGTTGCTGCC CACGGATGAG
    ACAGTCATGG ATGGCCTGCA TGGTGGCCAC ATCTCCATGT CCAATCTGAC ACTCTTGGTG
    AAGAGAGAAG ACAATGGCCT GTCCCTCACC TGCGAAGCCT TCAGTGATGC CTTCAGCAAG
    GAGACCTTCA AGAAGTCACT CACCTTGAAT GTAAAATACC CGGCCCAGAA GCTGTGGATT
    GAGGGACCCC CAGAGGGGCA GAGCATCCGG ACTGGAACTC GGGTGAGGCT GGTATGCTTG
    GCCATTGGAG GCAACCCAGA GCCCTCCCTC ACCTGGCTTA AGGATTCGCG CCCGGTGAAC
    GATCCTCGGC AGTCTCAGGA GCCCCGGCGT GTGCAGCTGG GCAGTGTGGA GAAGTCCGGC
    AGCACTTTCT CCCGCGAGCT GGTGCTGATC ATAGGCCCGC CGGACAACCT AGCCAAGTTC
    TCCTGCAAGG CGGGTCAGCT CAGTGCGTCT ACGCAGCTGG TGGTGCAGTT CCCCCCAACA
    AACCTGACCA TCCTGGCCAA CTCGTCCGCA CTGCGCCCAG GCGACGCCTT GAACTTGACC
    TGCGTCAGCA TCAGCAGCAA CCCTCCAGTT AACTTGTCTT TGGACAAGGA AGGAGAGAGG
    CTGGACGACG TGGCTGCAAA ACCCCAGAGC GCCCCATTCA AAGGCTCCGC TGCATCCAGG
    AGTGTTTTTC TTCGGGTGTC ATCCCGAGAC CACGGTCACC GGGTTACCTG CCGGGCCCAC
    AGCGAGGCAC TTCGTGAAAC CGTGAGCTCT TTCTACCGCC TCAACGTGTT ATACCCTCCA
    GAGTTCCTGG GAGAGCAAGT GCGGGCAGTG ACCGTGGTGG AGCAGGGCCA GGCACTGCTG
    CCTGTGTCCG TGTCTGCTAA CCCCGCCCCC GAGGCCTTCA ACTGGACCTT CCGAGGCTAC
    CGCCTCAGCC CAGCTGGGGG TCCCCGGCAC CGCATCCTGT CTGGAGGGGC ACTGCAGCTG
    TGGAATGTAA CCCGAGCTGA CGATGGCTTT TATCAGCTAC ACTGCCAGAA CTCGGAGGGC
    ACCGCCGAGG CGCTGTTGAA GCTGGACGTG CATTATGCTC CCACCATCCG TGCCCTGAAG
    GACCCTACTG AGGTGAATGT TGGGGGTTCT GTGGATATAG TCTGCACCGT CGATGCCAAT
    CCCATCCTCC CAGAGATGTT CAGCTGGGAG AGACTGGGGG AAGACGAGGA GGAACTGAAC
    CTGGACGACA TGGAGAAGAT GTCAAAGGGA TCCACAGGGC GTCTGCGGAT TCGCCAGGCC
    AAGCTGTCCC AGGCTGGCGC TTACCAGTGC ATCGTGGACA ACGGGGTGGC TCCCGCAGCC
    AGAGGACTGG TTCGTCTTGT TGTCCGATTT GCCCCCCAGG TGGATCATCC TACTCCCCTG
    ACGAAAGTGG CTGCTGCTGG GGACAGCACC AGCTCAGCCA CACTCCACTG CCGTGCCCGG
    GGGGTCCCCA ACATCGACTT CACTTGGACC AAAAATGGGG TCCCTCTGGA TCTCCAAGAC
    CCCAGGTACA CAGAGCACAA GTACCACCAG GGTGTGGTCC ACAGCAGCCT CCTGACCATT
    GCTAATGTGT CTGCAGCCCA GGACTATGCC CTCTTCAAAT GCACAGCCAC CAATGCCCTT
    GGTTCAGACC ACACCAACAT CCAGCTCGTC AGCATCAGCC GTCCTGACCC TCCACTGGGG
    CTGAAGGTTG TGAGCGTGAG CCCTCACTCG GTAGGACTGG AGTGGAAACC TGGCTTTGAT
    GGGGGTCTGC CTCAGAGGTT CCAAATCAGG TATGAGGCCC TCGAGAGCCC AGGATTCCTC
    TACATGGATG TCCTGCCTGC ACAGGCCACC ACCTTCACAC TGACTGGGCT GAAGCCTTCT
    ACACGGTACA GGATCTGGCT GTTGGCCAGC AATGCCCTGG GGGACAGTGG ATTGACGGAC
    AAGGGGATCC AGGTCTCCAT CACTACCCCA GGCCTGGACC AGGCTCCTGA AGACACAGAC
    CAGCCGCTGC CCACAGAGCA GCCTCCGGGA CCCCCGAGGC TGCCCCTGCT GCCTGTGCTC
    TTTGCGGTTG GTGGTCTTCT GTTGCTTTCC AATGCCTCCT GTGTTGGGGG ACTCCTCTGG
    CGGAGAAGAC TGAGGCGCCT TGCTGAGGAG ATCTCAGAGA AGACAGAGGC AGGGTCGGAG
    GAGGATCGAA TCAGGAATGA ATATGAGGAG AGTCAGTGGA CTGGGGACCG GGACACAAGA
    AGCTCCACGG TTAGCACAGC AGAAGTGGAC CCACACTACT ACTCCATGAG GGACTTCAGT
    CCCCAGCTTC CCCCAACACT GGAAGAGGTG TCATATCGCC AAGCCTTCAC AGGTATTGAA
    GATGAGGACA TGGCCTTCCC CGGACACCTG TATGACGAGG TGGAGAGAGT CTATGGCCCA
    CCTGGGGTCT GGGGACCCCT CTATGATGAA GTACAAATGG ACCCCTATGA CCTTCGCTGG
    CCTGAGGTCA AATATGAGGA CCCAAGGGGA ATCTACGACC AGGTGGCGGC AGACATGGAT
    GCTGGGGAGC CTGGTTCTCT GCCCTTTGAG CTGAGGGGAC ATCTGGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_062332.2
    CDS87..3857
    Translation

    Target ORF information:

    RefSeq Version NM_019459.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus nephrosis 1, nephrin (Nphs1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_019459.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGAGCTA AGGAAGCCAC AGTCAGAGGC CCCGGGGCAA GCCCAGTGCA CAGAACTTGC 
    CACCTGATTC CCCTGCTGCT CGCGGGAATG CTGACCACAG GCCTGGCCCA GTCGCCAGTC
    CCCACCTCAG CACCTCGAGG CTTCTGGGCT CTATCTGAAA ACCTGACTGT GGTGGAAGGG
    TCGACAGTTA AGCTGTGGTG TGGCGTCAGG GCCCCCGGCA GTGTGGTGCA GTGGGCTAAG
    GATGGGCTGC TTCTGGGTCC AAACCCTAAG ATTCCAGGCT TCCCAAGGTA CAGCCTGGAA
    GGAGACAGTG CTAAAGGTGA GTTCCACCTG CTTATTGAAG CCTGTGACCT CAGCGATGAT
    GCGGAGTACG AGTGCCAAGT CGGCCGCTCC GAGTTGGGTC CCGAGCTCGT GTCTCCCAGC
    GTAATCCTCT CCATCCTAGT TTCCCCCAAG GTGCTTCAGT TAACCCCTGA GGCAGGAAGC
    ACAGTTACCT GGGTAGCTGG ACAGGAATAT GTGGTCACCT GTGTGTCTGG GGATGCAAAG
    CCAGCACCTG ACATCATCTT CATCCAGGGT GGACGCACGG TAGAGGATGT CTCCTCCAGC
    GTGAATGAGG GATCAGAGGA GAAACTCTTC TTCACGGAAG CCGAAGCCAG GGTGACACCC
    CAGAGCTCAG ATAATGGGCA GCTGCTAGTC TGCGAGGGGT CCAACCCAGC CTTGGCCACT
    CCCATAAAGG CTTCGTTCAC CATGAATATC CTGTTCCCCC CAGGACCTCC TGTCATTGAT
    TGGCCAGGCC TGAATGAGGG GCATGTACGG GCAGGGGAGA ACCTGGAGCT ACCCTGCATA
    GCCAGAGGTG GAAATCCACC TGCGACCCTG CAGTGGCTGA AGAATGGTAA ACCAGTGTCC
    ATAGCTTGGG GCACAGAGCA TGCCCAGGCA GTGGCTCACA GTGTGCTGGT GATGACCGTT
    CGACCTGAAG ACCACGGAGC TCGGCTCAGC TGTCAGTCCT ACAACAGTGT GTCTGCAGAG
    ACCCAGGAGC GAAGCATCAC TCTGCAGGTC ACCTTTCCCC CCAGTGCCGT TACCATCCTG
    GGATCTACAT CACAGTCTGA GAACAAAAAT GTGACCCTTT GCTGCCTTAC CAAGTCCAGT
    CGCCCACGGG TCCTGCTGCG ATGGTGGTTG GGTGGACGGC AGTTGCTGCC CACGGATGAG
    ACAGTCATGG ATGGCCTGCA TGGTGGCCAC ATCTCCATGT CCAATCTGAC ACTCTTGGTG
    AAGAGAGAAG ACAATGGCCT GTCCCTCACC TGCGAAGCCT TCAGTGATGC CTTCAGCAAG
    GAGACCTTCA AGAAGTCACT CACCTTGAAT GTAAAATACC CGGCCCAGAA GCTGTGGATT
    GAGGGACCCC CAGAGGGGCA GAGCATCCGG ACTGGAACTC GGGTGAGGCT GGTATGCTTG
    GCCATTGGAG GCAACCCAGA GCCCTCCCTC ACCTGGCTTA AGGATTCGCG CCCGGTGAAC
    GATCCTCGGC AGTCTCAGGA GCCCCGGCGT GTGCAGCTGG GCAGTGTGGA GAAGTCCGGC
    AGCACTTTCT CCCGCGAGCT GGTGCTGATC ATAGGCCCGC CGGACAACCT AGCCAAGTTC
    TCCTGCAAGG CGGGTCAGCT CAGTGCGTCT ACGCAGCTGG TGGTGCAGTT CCCCCCAACA
    AACCTGACCA TCCTGGCCAA CTCGTCCGCA CTGCGCCCAG GCGACGCCTT GAACTTGACC
    TGCGTCAGCA TCAGCAGCAA CCCTCCAGTT AACTTGTCTT TGGACAAGGA AGGAGAGAGG
    CTGGACGACG TGGCTGCAAA ACCCCAGAGC GCCCCATTCA AAGGCTCCGC TGCATCCAGG
    AGTGTTTTTC TTCGGGTGTC ATCCCGAGAC CACGGTCACC GGGTTACCTG CCGGGCCCAC
    AGCGAGGCAC TTCGTGAAAC CGTGAGCTCT TTCTACCGCC TCAACGTGTT ATACCCTCCA
    GAGTTCCTGG GAGAGCAAGT GCGGGCAGTG ACCGTGGTGG AGCAGGGCCA GGCACTGCTG
    CCTGTGTCCG TGTCTGCTAA CCCCGCCCCC GAGGCCTTCA ACTGGACCTT CCGAGGCTAC
    CGCCTCAGCC CAGCTGGGGG TCCCCGGCAC CGCATCCTGT CTGGAGGGGC ACTGCAGCTG
    TGGAATGTAA CCCGAGCTGA CGATGGCTTT TATCAGCTAC ACTGCCAGAA CTCGGAGGGC
    ACCGCCGAGG CGCTGTTGAA GCTGGACGTG CATTATGCTC CCACCATCCG TGCCCTGAAG
    GACCCTACTG AGGTGAATGT TGGGGGTTCT GTGGATATAG TCTGCACCGT CGATGCCAAT
    CCCATCCTCC CAGAGATGTT CAGCTGGGAG AGACTGGGGG AAGACGAGGA GGAACTGAAC
    CTGGACGACA TGGAGAAGAT GTCAAAGGGA TCCACAGGGC GTCTGCGGAT TCGCCAGGCC
    AAGCTGTCCC AGGCTGGCGC TTACCAGTGC ATCGTGGACA ACGGGGTGGC TCCCGCAGCC
    AGAGGACTGG TTCGTCTTGT TGTCCGATTT GCCCCCCAGG TGGATCATCC TACTCCCCTG
    ACGAAAGTGG CTGCTGCTGG GGACAGCACC AGCTCAGCCA CACTCCACTG CCGTGCCCGG
    GGGGTCCCCA ACATCGACTT CACTTGGACC AAAAATGGGG TCCCTCTGGA TCTCCAAGAC
    CCCAGGTACA CAGAGCACAA GTACCACCAG GGTGTGGTCC ACAGCAGCCT CCTGACCATT
    GCTAATGTGT CTGCAGCCCA GGACTATGCC CTCTTCAAAT GCACAGCCAC CAATGCCCTT
    GGTTCAGACC ACACCAACAT CCAGCTCGTC AGCATCAGCC GTCCTGACCC TCCACTGGGG
    CTGAAGGTTG TGAGCGTGAG CCCTCACTCG GTAGGACTGG AGTGGAAACC TGGCTTTGAT
    GGGGGTCTGC CTCAGAGGTT CCAAATCAGG TATGAGGCCC TCGAGAGCCC AGGATTCCTC
    TACATGGATG TCCTGCCTGC ACAGGCCACC ACCTTCACAC TGACTGGGCT GAAGCCTTCT
    ACACGGTACA GGATCTGGCT GTTGGCCAGC AATGCCCTGG GGGACAGTGG ATTGACGGAC
    AAGGGGATCC AGGTCTCCAT CACTACCCCA GGCCTGGACC AGGCTCCTGA AGACACAGAC
    CAGCCGCTGC CCACAGAGCA GCCTCCGGGA CCCCCGAGGC TGCCCCTGCT GCCTGTGCTC
    TTTGCGGTTG GTGGTCTTCT GTTGCTTTCC AATGCCTCCT GTGTTGGGGG ACTCCTCTGG
    CGGAGAAGAC TGAGGCGCCT TGCTGAGGAG ATCTCAGAGA AGACAGAGGC AGGGTCGGAG
    GAGGATCGAA TCAGGAATGA ATATGAGGAG AGTCAGTGGA CTGGGGACCG GGACACAAGA
    AGCTCCACGG TTAGCACAGC AGAAGTGGAC CCACACTACT ACTCCATGAG GGACTTCAGT
    CCCCAGCTTC CCCCAACACT GGAAGAGGTG TCATATCGCC AAGCCTTCAC AGGTATTGAA
    GATGAGGACA TGGCCTTCCC CGGACACCTG TATGACGAGG TGGAGAGAGT CTATGGCCCA
    CCTGGGGTCT GGGGACCCCT CTATGATGAA GTACAAATGG ACCCCTATGA CCTTCGCTGG
    CCTGAGGTCA AATATGAGGA CCCAAGGGGA ATCTACGACC AGGTGGCGGC AGACATGGAT
    GCTGGGGAGC CTGGTTCTCT GCCCTTTGAG CTGAGGGGAC ATCTGGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu69564
    Clone ID Related Accession (Same CDS sequence) XM_011250647.2 , XM_011250647.1
    Accession Version XM_011250647.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product nephrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250647.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAAAAAT GGAGAGCCTG GGATCCCCAG AGCATTCAGA GAAGGAAAAC AGCCAAAGGC 
    CTGGCCCAGT CGCCAGTCCC CACCTCAGCA CCTCGAGGCT TCTGGGCTCT ATCTGAAAAC
    CTGACTGTGG TGGAAGGGTC GACAGTTAAG CTGTGGTGTG GCGTCAGGGC CCCCGGCAGT
    GTGGTGCAGT GGGCTAAGGA TGGGCTGCTT CTGGGTCCAA ACCCTAAGAT TCCAGGCTTC
    CCAAGGTACA GCCTGGAAGG AGACAGTGCT AAAGGTGAGT TCCACCTGCT TATTGAAGCC
    TGTGACCTCA GCGATGATGC GGAGTACGAG TGCCAAGTCG GCCGCTCCGA GTTGGGTCCC
    GAGCTCGTGT CTCCCAGCGT AATCCTCTCC ATCCTAGTTT CCCCCAAGGT GCTTCAGTTA
    ACCCCTGAGG CAGGAAGCAC AGTTACCTGG GTAGCTGGAC AGGAATATGT GGTCACCTGT
    GTGTCTGGGG ATGCAAAGCC AGCACCTGAC ATCATCTTCA TCCAGGGTGG ACGCACGGTA
    GAGGATGTCT CCTCCAGCGT GAATGAGGGA TCAGAGGAGA AACTCTTCTT CACGGAAGCC
    GAAGCCAGGG TGACACCCCA GAGCTCAGAT AATGGGCAGC TGCTAGTCTG CGAGGGGTCC
    AACCCAGCCT TGGCCACTCC CATAAAGGCT TCGTTCACCA TGAATATCCT GTTCCCCCCA
    GGACCTCCTG TCATTGATTG GCCAGGCCTG AATGAGGGGC ATGTACGGGC AGGGGAGAAC
    CTGGAGCTAC CCTGCATAGC CAGAGGTGGA AATCCACCTG CGACCCTGCA GTGGCTGAAG
    AATGGTAAAC CAGTGTCCAT AGCTTGGGGC ACAGAGCATG CCCAGGCAGT GGCTCACAGT
    GTGCTGGTGA TGACCGTTCG ACCTGAAGAC CACGGAGCTC GGCTCAGCTG TCAGTCCTAC
    AACAGTGTGT CTGCAGAGAC CCAGGAGCGA AGCATCACTC TGCAGGTCAC CTTTCCCCCC
    AGTGCCGTTA CCATCCTGGG ATCTACATCA CAGTCTGAGA ACAAAAATGT GACCCTTTGC
    TGCCTTACCA AGTCCAGTCG CCCACGGGTC CTGCTGCGAT GGTGGTTGGG TGGACGGCAG
    TTGCTGCCCA CGGATGAGAC AGTCATGGAT GGCCTGCATG GTGGCCACAT CTCCATGTCC
    AATCTGACAC TCTTGGTGAA GAGAGAAGAC AATGGCCTGT CCCTCACCTG CGAAGCCTTC
    AGTGATGCCT TCAGCAAGGA GACCTTCAAG AAGTCACTCA CCTTGAATGT AAAATACCCG
    GCCCAGAAGC TGTGGATTGA GGGACCCCCA GAGGGGCAGA GCATCCGGAC TGGAACTCGG
    GTGAGGCTGG TATGCTTGGC CATTGGAGGC AACCCAGAGC CCTCCCTCAC CTGGCTTAAG
    GATTCGCGCC CGGTGAACGA TCCTCGGCAG TCTCAGGAGC CCCGGCGTGT GCAGCTGGGC
    AGTGTGGAGA AGTCCGGCAG CACTTTCTCC CGCGAGCTGG TGCTGATCAT AGGCCCGCCG
    GACAACCTAG CCAAGTTCTC CTGCAAGGCG GGTCAGCTCA GTGCGTCTAC GCAGCTGGTG
    GTGCAGTTCC CCCCAACAAA CCTGACCATC CTGGCCAACT CGTCCGCACT GCGCCCAGGC
    GACGCCTTGA ACTTGACCTG CGTCAGCATC AGCAGCAACC CTCCAGTTAA CTTGTCTTTG
    GACAAGGAAG GAGAGAGGCT GGACGACGTG GCTGCAAAAC CCCAGAGCGC CCCATTCAAA
    GGCTCCGCTG CATCCAGGAG TGTTTTTCTT CGGGTGTCAT CCCGAGACCA CGGTCACCGG
    GTTACCTGCC GGGCCCACAG CGAGGCACTT CGTGAAACCG TGAGCTCTTT CTACCGCCTC
    AACGTGTTAT ACCCTCCAGA GTTCCTGGGA GAGCAAGTGC GGGCAGTGAC CGTGGTGGAG
    CAGGGCCAGG CACTGCTGCC TGTGTCCGTG TCTGCTAACC CCGCCCCCGA GGCCTTCAAC
    TGGACCTTCC GAGGCTACCG CCTCAGCCCA GCTGGGGGTC CCCGGCACCG CATCCTGTCT
    GGAGGGGCAC TGCAGCTGTG GAATGTAACC CGAGCTGACG ATGGCTTTTA TCAGCTACAC
    TGCCAGAACT CGGAGGGCAC CGCCGAGGCG CTGTTGAAGC TGGACGTGCA TTATGCTCCC
    ACCATCCGTG CCCTGAAGGA CCCTACTGAG GTGAATGTTG GGGGTTCTGT GGATATAGTC
    TGCACCGTCG ATGCCAATCC CATCCTCCCA GAGATGTTCA GCTGGGAGAG ACTGGGGGAA
    GACGAGGAGG AACTGAACCT GGACGACATG GAGAAGATGT CAAAGGGATC CACAGGGCGT
    CTGCGGATTC GCCAGGCCAA GCTGTCCCAG GCTGGCGCTT ACCAGTGCAT CGTGGACAAC
    GGGGTGGCTC CCGCAGCCAG AGGACTGGTT CGTCTTGTTG TCCGATTTGC CCCCCAGGTG
    GATCATCCTA CTCCCCTGAC GAAAGTGGCT GCTGCTGGGG ACAGCACCAG CTCAGCCACA
    CTCCACTGCC GTGCCCGGGG GGTCCCCAAC ATCGACTTCA CTTGGACCAA AAATGGGGTC
    CCTCTGGATC TCCAAGACCC CAGGTACACA GAGCACAAGT ACCACCAGGG TGTGGTCCAC
    AGCAGCCTCC TGACCATTGC TAATGTGTCT GCAGCCCAGG ACTATGCCCT CTTCAAATGC
    ACAGCCACCA ATGCCCTTGG TTCAGACCAC ACCAACATCC AGCTCGTCAG CATCAGCCGT
    CCTGACCCTC CACTGGGGCT GAAGGTTGTG AGCGTGAGCC CTCACTCGGT AGGACTGGAG
    TGGAAACCTG GCTTTGATGG GGGTCTGCCT CAGAGGTTCC AAATCAGGTA TGAGGCCCTC
    GAGAGCCCAG GATTCCTCTA CATGGATGTC CTGCCTGCAC AGGCCACCAC CTTCACACTG
    ACTGGGCTGA AGCCTTCTAC ACGGTACAGG ATCTGGCTGT TGGCCAGCAA TGCCCTGGGG
    GACAGTGGAT TGACGGACAA GGGGATCCAG GTCTCCATCA CTACCCCAGG CCTGGACCAG
    GCTCCTGAAG ACACAGACCA GCCGCTGCCC ACAGAGCAGC CTCCGGGACC CCCGAGGCTG
    CCCCTGCTGC CTGTGCTCTT TGCGGTTGGT GGTCTTCTGT TGCTTTCCAA TGCCTCCTGT
    GTTGGGGGAC TCCTCTGGCG GAGAAGACTG AGGCGCCTTG CTGAGGAGAT CTCAGAGAAG
    ACAGAGGCAG GGTCGGAGGA GGATCGAATC AGGAATGAAT ATGAGGAGAG TCAGTGGACT
    GGGGACCGGG ACACAAGAAG CTCCACGGTT AGCACAGCAG AAGTGGACCC ACACTACTAC
    TCCATGAGGG ACTTCAGTCC CCAGCTTCCC CCAACACTGG AAGAGGTGTC ATATCGCCAA
    GCCTTCACAG GTATTGAAGA TGAGGACATG GCCTTCCCCG GACACCTGTA TGACGAGGTG
    GAGAGAGTCT ATGGCCCACC TGGGGTCTGG GGACCCCTCT ATGATGAAGT ACAAATGGAC
    CCCTATGACC TTCGCTGGCC TGAGGTCAAA TATGAGGACC CAAGGGGAAT CTACGACCAG
    GTGGCGGCAG ACATGGATGC TGGGGAGCCT GGTTCTCTGC CCTTTGAGCT GAGGGGACAT
    CTGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011248949.1
    CDS257..3985
    Translation

    Target ORF information:

    RefSeq Version XM_011250647.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus nephrosis 1, nephrin (Nphs1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011250647.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAAAAAT GGAGAGCCTG GGATCCCCAG AGCATTCAGA GAAGGAAAAC AGCCAAAGGC 
    CTGGCCCAGT CGCCAGTCCC CACCTCAGCA CCTCGAGGCT TCTGGGCTCT ATCTGAAAAC
    CTGACTGTGG TGGAAGGGTC GACAGTTAAG CTGTGGTGTG GCGTCAGGGC CCCCGGCAGT
    GTGGTGCAGT GGGCTAAGGA TGGGCTGCTT CTGGGTCCAA ACCCTAAGAT TCCAGGCTTC
    CCAAGGTACA GCCTGGAAGG AGACAGTGCT AAAGGTGAGT TCCACCTGCT TATTGAAGCC
    TGTGACCTCA GCGATGATGC GGAGTACGAG TGCCAAGTCG GCCGCTCCGA GTTGGGTCCC
    GAGCTCGTGT CTCCCAGCGT AATCCTCTCC ATCCTAGTTT CCCCCAAGGT GCTTCAGTTA
    ACCCCTGAGG CAGGAAGCAC AGTTACCTGG GTAGCTGGAC AGGAATATGT GGTCACCTGT
    GTGTCTGGGG ATGCAAAGCC AGCACCTGAC ATCATCTTCA TCCAGGGTGG ACGCACGGTA
    GAGGATGTCT CCTCCAGCGT GAATGAGGGA TCAGAGGAGA AACTCTTCTT CACGGAAGCC
    GAAGCCAGGG TGACACCCCA GAGCTCAGAT AATGGGCAGC TGCTAGTCTG CGAGGGGTCC
    AACCCAGCCT TGGCCACTCC CATAAAGGCT TCGTTCACCA TGAATATCCT GTTCCCCCCA
    GGACCTCCTG TCATTGATTG GCCAGGCCTG AATGAGGGGC ATGTACGGGC AGGGGAGAAC
    CTGGAGCTAC CCTGCATAGC CAGAGGTGGA AATCCACCTG CGACCCTGCA GTGGCTGAAG
    AATGGTAAAC CAGTGTCCAT AGCTTGGGGC ACAGAGCATG CCCAGGCAGT GGCTCACAGT
    GTGCTGGTGA TGACCGTTCG ACCTGAAGAC CACGGAGCTC GGCTCAGCTG TCAGTCCTAC
    AACAGTGTGT CTGCAGAGAC CCAGGAGCGA AGCATCACTC TGCAGGTCAC CTTTCCCCCC
    AGTGCCGTTA CCATCCTGGG ATCTACATCA CAGTCTGAGA ACAAAAATGT GACCCTTTGC
    TGCCTTACCA AGTCCAGTCG CCCACGGGTC CTGCTGCGAT GGTGGTTGGG TGGACGGCAG
    TTGCTGCCCA CGGATGAGAC AGTCATGGAT GGCCTGCATG GTGGCCACAT CTCCATGTCC
    AATCTGACAC TCTTGGTGAA GAGAGAAGAC AATGGCCTGT CCCTCACCTG CGAAGCCTTC
    AGTGATGCCT TCAGCAAGGA GACCTTCAAG AAGTCACTCA CCTTGAATGT AAAATACCCG
    GCCCAGAAGC TGTGGATTGA GGGACCCCCA GAGGGGCAGA GCATCCGGAC TGGAACTCGG
    GTGAGGCTGG TATGCTTGGC CATTGGAGGC AACCCAGAGC CCTCCCTCAC CTGGCTTAAG
    GATTCGCGCC CGGTGAACGA TCCTCGGCAG TCTCAGGAGC CCCGGCGTGT GCAGCTGGGC
    AGTGTGGAGA AGTCCGGCAG CACTTTCTCC CGCGAGCTGG TGCTGATCAT AGGCCCGCCG
    GACAACCTAG CCAAGTTCTC CTGCAAGGCG GGTCAGCTCA GTGCGTCTAC GCAGCTGGTG
    GTGCAGTTCC CCCCAACAAA CCTGACCATC CTGGCCAACT CGTCCGCACT GCGCCCAGGC
    GACGCCTTGA ACTTGACCTG CGTCAGCATC AGCAGCAACC CTCCAGTTAA CTTGTCTTTG
    GACAAGGAAG GAGAGAGGCT GGACGACGTG GCTGCAAAAC CCCAGAGCGC CCCATTCAAA
    GGCTCCGCTG CATCCAGGAG TGTTTTTCTT CGGGTGTCAT CCCGAGACCA CGGTCACCGG
    GTTACCTGCC GGGCCCACAG CGAGGCACTT CGTGAAACCG TGAGCTCTTT CTACCGCCTC
    AACGTGTTAT ACCCTCCAGA GTTCCTGGGA GAGCAAGTGC GGGCAGTGAC CGTGGTGGAG
    CAGGGCCAGG CACTGCTGCC TGTGTCCGTG TCTGCTAACC CCGCCCCCGA GGCCTTCAAC
    TGGACCTTCC GAGGCTACCG CCTCAGCCCA GCTGGGGGTC CCCGGCACCG CATCCTGTCT
    GGAGGGGCAC TGCAGCTGTG GAATGTAACC CGAGCTGACG ATGGCTTTTA TCAGCTACAC
    TGCCAGAACT CGGAGGGCAC CGCCGAGGCG CTGTTGAAGC TGGACGTGCA TTATGCTCCC
    ACCATCCGTG CCCTGAAGGA CCCTACTGAG GTGAATGTTG GGGGTTCTGT GGATATAGTC
    TGCACCGTCG ATGCCAATCC CATCCTCCCA GAGATGTTCA GCTGGGAGAG ACTGGGGGAA
    GACGAGGAGG AACTGAACCT GGACGACATG GAGAAGATGT CAAAGGGATC CACAGGGCGT
    CTGCGGATTC GCCAGGCCAA GCTGTCCCAG GCTGGCGCTT ACCAGTGCAT CGTGGACAAC
    GGGGTGGCTC CCGCAGCCAG AGGACTGGTT CGTCTTGTTG TCCGATTTGC CCCCCAGGTG
    GATCATCCTA CTCCCCTGAC GAAAGTGGCT GCTGCTGGGG ACAGCACCAG CTCAGCCACA
    CTCCACTGCC GTGCCCGGGG GGTCCCCAAC ATCGACTTCA CTTGGACCAA AAATGGGGTC
    CCTCTGGATC TCCAAGACCC CAGGTACACA GAGCACAAGT ACCACCAGGG TGTGGTCCAC
    AGCAGCCTCC TGACCATTGC TAATGTGTCT GCAGCCCAGG ACTATGCCCT CTTCAAATGC
    ACAGCCACCA ATGCCCTTGG TTCAGACCAC ACCAACATCC AGCTCGTCAG CATCAGCCGT
    CCTGACCCTC CACTGGGGCT GAAGGTTGTG AGCGTGAGCC CTCACTCGGT AGGACTGGAG
    TGGAAACCTG GCTTTGATGG GGGTCTGCCT CAGAGGTTCC AAATCAGGTA TGAGGCCCTC
    GAGAGCCCAG GATTCCTCTA CATGGATGTC CTGCCTGCAC AGGCCACCAC CTTCACACTG
    ACTGGGCTGA AGCCTTCTAC ACGGTACAGG ATCTGGCTGT TGGCCAGCAA TGCCCTGGGG
    GACAGTGGAT TGACGGACAA GGGGATCCAG GTCTCCATCA CTACCCCAGG CCTGGACCAG
    GCTCCTGAAG ACACAGACCA GCCGCTGCCC ACAGAGCAGC CTCCGGGACC CCCGAGGCTG
    CCCCTGCTGC CTGTGCTCTT TGCGGTTGGT GGTCTTCTGT TGCTTTCCAA TGCCTCCTGT
    GTTGGGGGAC TCCTCTGGCG GAGAAGACTG AGGCGCCTTG CTGAGGAGAT CTCAGAGAAG
    ACAGAGGCAG GGTCGGAGGA GGATCGAATC AGGAATGAAT ATGAGGAGAG TCAGTGGACT
    GGGGACCGGG ACACAAGAAG CTCCACGGTT AGCACAGCAG AAGTGGACCC ACACTACTAC
    TCCATGAGGG ACTTCAGTCC CCAGCTTCCC CCAACACTGG AAGAGGTGTC ATATCGCCAA
    GCCTTCACAG GTATTGAAGA TGAGGACATG GCCTTCCCCG GACACCTGTA TGACGAGGTG
    GAGAGAGTCT ATGGCCCACC TGGGGTCTGG GGACCCCTCT ATGATGAAGT ACAAATGGAC
    CCCTATGACC TTCGCTGGCC TGAGGTCAAA TATGAGGACC CAAGGGGAAT CTACGACCAG
    GTGGCGGCAG ACATGGATGC TGGGGAGCCT GGTTCTCTGC CCTTTGAGCT GAGGGGACAT
    CTGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu69564
    Clone ID Related Accession (Same CDS sequence) XM_011250647.2 , XM_011250647.1
    Accession Version XM_011250647.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product nephrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAAAAAT GGAGAGCCTG GGATCCCCAG AGCATTCAGA GAAGGAAAAC AGCCAAAGGC 
    CTGGCCCAGT CGCCAGTCCC CACCTCAGCA CCTCGAGGCT TCTGGGCTCT ATCTGAAAAC
    CTGACTGTGG TGGAAGGGTC GACAGTTAAG CTGTGGTGTG GCGTCAGGGC CCCCGGCAGT
    GTGGTGCAGT GGGCTAAGGA TGGGCTGCTT CTGGGTCCAA ACCCTAAGAT TCCAGGCTTC
    CCAAGGTACA GCCTGGAAGG AGACAGTGCT AAAGGTGAGT TCCACCTGCT TATTGAAGCC
    TGTGACCTCA GCGATGATGC GGAGTACGAG TGCCAAGTCG GCCGCTCCGA GTTGGGTCCC
    GAGCTCGTGT CTCCCAGCGT AATCCTCTCC ATCCTAGTTT CCCCCAAGGT GCTTCAGTTA
    ACCCCTGAGG CAGGAAGCAC AGTTACCTGG GTAGCTGGAC AGGAATATGT GGTCACCTGT
    GTGTCTGGGG ATGCAAAGCC AGCACCTGAC ATCATCTTCA TCCAGGGTGG ACGCACGGTA
    GAGGATGTCT CCTCCAGCGT GAATGAGGGA TCAGAGGAGA AACTCTTCTT CACGGAAGCC
    GAAGCCAGGG TGACACCCCA GAGCTCAGAT AATGGGCAGC TGCTAGTCTG CGAGGGGTCC
    AACCCAGCCT TGGCCACTCC CATAAAGGCT TCGTTCACCA TGAATATCCT GTTCCCCCCA
    GGACCTCCTG TCATTGATTG GCCAGGCCTG AATGAGGGGC ATGTACGGGC AGGGGAGAAC
    CTGGAGCTAC CCTGCATAGC CAGAGGTGGA AATCCACCTG CGACCCTGCA GTGGCTGAAG
    AATGGTAAAC CAGTGTCCAT AGCTTGGGGC ACAGAGCATG CCCAGGCAGT GGCTCACAGT
    GTGCTGGTGA TGACCGTTCG ACCTGAAGAC CACGGAGCTC GGCTCAGCTG TCAGTCCTAC
    AACAGTGTGT CTGCAGAGAC CCAGGAGCGA AGCATCACTC TGCAGGTCAC CTTTCCCCCC
    AGTGCCGTTA CCATCCTGGG ATCTACATCA CAGTCTGAGA ACAAAAATGT GACCCTTTGC
    TGCCTTACCA AGTCCAGTCG CCCACGGGTC CTGCTGCGAT GGTGGTTGGG TGGACGGCAG
    TTGCTGCCCA CGGATGAGAC AGTCATGGAT GGCCTGCATG GTGGCCACAT CTCCATGTCC
    AATCTGACAC TCTTGGTGAA GAGAGAAGAC AATGGCCTGT CCCTCACCTG CGAAGCCTTC
    AGTGATGCCT TCAGCAAGGA GACCTTCAAG AAGTCACTCA CCTTGAATGT AAAATACCCG
    GCCCAGAAGC TGTGGATTGA GGGACCCCCA GAGGGGCAGA GCATCCGGAC TGGAACTCGG
    GTGAGGCTGG TATGCTTGGC CATTGGAGGC AACCCAGAGC CCTCCCTCAC CTGGCTTAAG
    GATTCGCGCC CGGTGAACGA TCCTCGGCAG TCTCAGGAGC CCCGGCGTGT GCAGCTGGGC
    AGTGTGGAGA AGTCCGGCAG CACTTTCTCC CGCGAGCTGG TGCTGATCAT AGGCCCGCCG
    GACAACCTAG CCAAGTTCTC CTGCAAGGCG GGTCAGCTCA GTGCGTCTAC GCAGCTGGTG
    GTGCAGTTCC CCCCAACAAA CCTGACCATC CTGGCCAACT CGTCCGCACT GCGCCCAGGC
    GACGCCTTGA ACTTGACCTG CGTCAGCATC AGCAGCAACC CTCCAGTTAA CTTGTCTTTG
    GACAAGGAAG GAGAGAGGCT GGACGACGTG GCTGCAAAAC CCCAGAGCGC CCCATTCAAA
    GGCTCCGCTG CATCCAGGAG TGTTTTTCTT CGGGTGTCAT CCCGAGACCA CGGTCACCGG
    GTTACCTGCC GGGCCCACAG CGAGGCACTT CGTGAAACCG TGAGCTCTTT CTACCGCCTC
    AACGTGTTAT ACCCTCCAGA GTTCCTGGGA GAGCAAGTGC GGGCAGTGAC CGTGGTGGAG
    CAGGGCCAGG CACTGCTGCC TGTGTCCGTG TCTGCTAACC CCGCCCCCGA GGCCTTCAAC
    TGGACCTTCC GAGGCTACCG CCTCAGCCCA GCTGGGGGTC CCCGGCACCG CATCCTGTCT
    GGAGGGGCAC TGCAGCTGTG GAATGTAACC CGAGCTGACG ATGGCTTTTA TCAGCTACAC
    TGCCAGAACT CGGAGGGCAC CGCCGAGGCG CTGTTGAAGC TGGACGTGCA TTATGCTCCC
    ACCATCCGTG CCCTGAAGGA CCCTACTGAG GTGAATGTTG GGGGTTCTGT GGATATAGTC
    TGCACCGTCG ATGCCAATCC CATCCTCCCA GAGATGTTCA GCTGGGAGAG ACTGGGGGAA
    GACGAGGAGG AACTGAACCT GGACGACATG GAGAAGATGT CAAAGGGATC CACAGGGCGT
    CTGCGGATTC GCCAGGCCAA GCTGTCCCAG GCTGGCGCTT ACCAGTGCAT CGTGGACAAC
    GGGGTGGCTC CCGCAGCCAG AGGACTGGTT CGTCTTGTTG TCCGATTTGC CCCCCAGGTG
    GATCATCCTA CTCCCCTGAC GAAAGTGGCT GCTGCTGGGG ACAGCACCAG CTCAGCCACA
    CTCCACTGCC GTGCCCGGGG GGTCCCCAAC ATCGACTTCA CTTGGACCAA AAATGGGGTC
    CCTCTGGATC TCCAAGACCC CAGGTACACA GAGCACAAGT ACCACCAGGG TGTGGTCCAC
    AGCAGCCTCC TGACCATTGC TAATGTGTCT GCAGCCCAGG ACTATGCCCT CTTCAAATGC
    ACAGCCACCA ATGCCCTTGG TTCAGACCAC ACCAACATCC AGCTCGTCAG CATCAGCCGT
    CCTGACCCTC CACTGGGGCT GAAGGTTGTG AGCGTGAGCC CTCACTCGGT AGGACTGGAG
    TGGAAACCTG GCTTTGATGG GGGTCTGCCT CAGAGGTTCC AAATCAGGTA TGAGGCCCTC
    GAGAGCCCAG GATTCCTCTA CATGGATGTC CTGCCTGCAC AGGCCACCAC CTTCACACTG
    ACTGGGCTGA AGCCTTCTAC ACGGTACAGG ATCTGGCTGT TGGCCAGCAA TGCCCTGGGG
    GACAGTGGAT TGACGGACAA GGGGATCCAG GTCTCCATCA CTACCCCAGG CCTGGACCAG
    GCTCCTGAAG ACACAGACCA GCCGCTGCCC ACAGAGCAGC CTCCGGGACC CCCGAGGCTG
    CCCCTGCTGC CTGTGCTCTT TGCGGTTGGT GGTCTTCTGT TGCTTTCCAA TGCCTCCTGT
    GTTGGGGGAC TCCTCTGGCG GAGAAGACTG AGGCGCCTTG CTGAGGAGAT CTCAGAGAAG
    ACAGAGGCAG GGTCGGAGGA GGATCGAATC AGGAATGAAT ATGAGGAGAG TCAGTGGACT
    GGGGACCGGG ACACAAGAAG CTCCACGGTT AGCACAGCAG AAGTGGACCC ACACTACTAC
    TCCATGAGGG ACTTCAGTCC CCAGCTTCCC CCAACACTGG AAGAGGTGTC ATATCGCCAA
    GCCTTCACAG GTATTGAAGA TGAGGACATG GCCTTCCCCG GACACCTGTA TGACGAGGTG
    GAGAGAGTCT ATGGCCCACC TGGGGTCTGG GGACCCCTCT ATGATGAAGT ACAAATGGAC
    CCCTATGACC TTCGCTGGCC TGAGGTCAAA TATGAGGACC CAAGGGGAAT CTACGACCAG
    GTGGCGGCAG ACATGGATGC TGGGGAGCCT GGTTCTCTGC CCTTTGAGCT GAGGGGACAT
    CTGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011248949.1
    CDS258..3986
    Translation

    Target ORF information:

    RefSeq Version XM_011250647.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus nephrosis 1, nephrin (Nphs1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011250647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAAAAAT GGAGAGCCTG GGATCCCCAG AGCATTCAGA GAAGGAAAAC AGCCAAAGGC 
    CTGGCCCAGT CGCCAGTCCC CACCTCAGCA CCTCGAGGCT TCTGGGCTCT ATCTGAAAAC
    CTGACTGTGG TGGAAGGGTC GACAGTTAAG CTGTGGTGTG GCGTCAGGGC CCCCGGCAGT
    GTGGTGCAGT GGGCTAAGGA TGGGCTGCTT CTGGGTCCAA ACCCTAAGAT TCCAGGCTTC
    CCAAGGTACA GCCTGGAAGG AGACAGTGCT AAAGGTGAGT TCCACCTGCT TATTGAAGCC
    TGTGACCTCA GCGATGATGC GGAGTACGAG TGCCAAGTCG GCCGCTCCGA GTTGGGTCCC
    GAGCTCGTGT CTCCCAGCGT AATCCTCTCC ATCCTAGTTT CCCCCAAGGT GCTTCAGTTA
    ACCCCTGAGG CAGGAAGCAC AGTTACCTGG GTAGCTGGAC AGGAATATGT GGTCACCTGT
    GTGTCTGGGG ATGCAAAGCC AGCACCTGAC ATCATCTTCA TCCAGGGTGG ACGCACGGTA
    GAGGATGTCT CCTCCAGCGT GAATGAGGGA TCAGAGGAGA AACTCTTCTT CACGGAAGCC
    GAAGCCAGGG TGACACCCCA GAGCTCAGAT AATGGGCAGC TGCTAGTCTG CGAGGGGTCC
    AACCCAGCCT TGGCCACTCC CATAAAGGCT TCGTTCACCA TGAATATCCT GTTCCCCCCA
    GGACCTCCTG TCATTGATTG GCCAGGCCTG AATGAGGGGC ATGTACGGGC AGGGGAGAAC
    CTGGAGCTAC CCTGCATAGC CAGAGGTGGA AATCCACCTG CGACCCTGCA GTGGCTGAAG
    AATGGTAAAC CAGTGTCCAT AGCTTGGGGC ACAGAGCATG CCCAGGCAGT GGCTCACAGT
    GTGCTGGTGA TGACCGTTCG ACCTGAAGAC CACGGAGCTC GGCTCAGCTG TCAGTCCTAC
    AACAGTGTGT CTGCAGAGAC CCAGGAGCGA AGCATCACTC TGCAGGTCAC CTTTCCCCCC
    AGTGCCGTTA CCATCCTGGG ATCTACATCA CAGTCTGAGA ACAAAAATGT GACCCTTTGC
    TGCCTTACCA AGTCCAGTCG CCCACGGGTC CTGCTGCGAT GGTGGTTGGG TGGACGGCAG
    TTGCTGCCCA CGGATGAGAC AGTCATGGAT GGCCTGCATG GTGGCCACAT CTCCATGTCC
    AATCTGACAC TCTTGGTGAA GAGAGAAGAC AATGGCCTGT CCCTCACCTG CGAAGCCTTC
    AGTGATGCCT TCAGCAAGGA GACCTTCAAG AAGTCACTCA CCTTGAATGT AAAATACCCG
    GCCCAGAAGC TGTGGATTGA GGGACCCCCA GAGGGGCAGA GCATCCGGAC TGGAACTCGG
    GTGAGGCTGG TATGCTTGGC CATTGGAGGC AACCCAGAGC CCTCCCTCAC CTGGCTTAAG
    GATTCGCGCC CGGTGAACGA TCCTCGGCAG TCTCAGGAGC CCCGGCGTGT GCAGCTGGGC
    AGTGTGGAGA AGTCCGGCAG CACTTTCTCC CGCGAGCTGG TGCTGATCAT AGGCCCGCCG
    GACAACCTAG CCAAGTTCTC CTGCAAGGCG GGTCAGCTCA GTGCGTCTAC GCAGCTGGTG
    GTGCAGTTCC CCCCAACAAA CCTGACCATC CTGGCCAACT CGTCCGCACT GCGCCCAGGC
    GACGCCTTGA ACTTGACCTG CGTCAGCATC AGCAGCAACC CTCCAGTTAA CTTGTCTTTG
    GACAAGGAAG GAGAGAGGCT GGACGACGTG GCTGCAAAAC CCCAGAGCGC CCCATTCAAA
    GGCTCCGCTG CATCCAGGAG TGTTTTTCTT CGGGTGTCAT CCCGAGACCA CGGTCACCGG
    GTTACCTGCC GGGCCCACAG CGAGGCACTT CGTGAAACCG TGAGCTCTTT CTACCGCCTC
    AACGTGTTAT ACCCTCCAGA GTTCCTGGGA GAGCAAGTGC GGGCAGTGAC CGTGGTGGAG
    CAGGGCCAGG CACTGCTGCC TGTGTCCGTG TCTGCTAACC CCGCCCCCGA GGCCTTCAAC
    TGGACCTTCC GAGGCTACCG CCTCAGCCCA GCTGGGGGTC CCCGGCACCG CATCCTGTCT
    GGAGGGGCAC TGCAGCTGTG GAATGTAACC CGAGCTGACG ATGGCTTTTA TCAGCTACAC
    TGCCAGAACT CGGAGGGCAC CGCCGAGGCG CTGTTGAAGC TGGACGTGCA TTATGCTCCC
    ACCATCCGTG CCCTGAAGGA CCCTACTGAG GTGAATGTTG GGGGTTCTGT GGATATAGTC
    TGCACCGTCG ATGCCAATCC CATCCTCCCA GAGATGTTCA GCTGGGAGAG ACTGGGGGAA
    GACGAGGAGG AACTGAACCT GGACGACATG GAGAAGATGT CAAAGGGATC CACAGGGCGT
    CTGCGGATTC GCCAGGCCAA GCTGTCCCAG GCTGGCGCTT ACCAGTGCAT CGTGGACAAC
    GGGGTGGCTC CCGCAGCCAG AGGACTGGTT CGTCTTGTTG TCCGATTTGC CCCCCAGGTG
    GATCATCCTA CTCCCCTGAC GAAAGTGGCT GCTGCTGGGG ACAGCACCAG CTCAGCCACA
    CTCCACTGCC GTGCCCGGGG GGTCCCCAAC ATCGACTTCA CTTGGACCAA AAATGGGGTC
    CCTCTGGATC TCCAAGACCC CAGGTACACA GAGCACAAGT ACCACCAGGG TGTGGTCCAC
    AGCAGCCTCC TGACCATTGC TAATGTGTCT GCAGCCCAGG ACTATGCCCT CTTCAAATGC
    ACAGCCACCA ATGCCCTTGG TTCAGACCAC ACCAACATCC AGCTCGTCAG CATCAGCCGT
    CCTGACCCTC CACTGGGGCT GAAGGTTGTG AGCGTGAGCC CTCACTCGGT AGGACTGGAG
    TGGAAACCTG GCTTTGATGG GGGTCTGCCT CAGAGGTTCC AAATCAGGTA TGAGGCCCTC
    GAGAGCCCAG GATTCCTCTA CATGGATGTC CTGCCTGCAC AGGCCACCAC CTTCACACTG
    ACTGGGCTGA AGCCTTCTAC ACGGTACAGG ATCTGGCTGT TGGCCAGCAA TGCCCTGGGG
    GACAGTGGAT TGACGGACAA GGGGATCCAG GTCTCCATCA CTACCCCAGG CCTGGACCAG
    GCTCCTGAAG ACACAGACCA GCCGCTGCCC ACAGAGCAGC CTCCGGGACC CCCGAGGCTG
    CCCCTGCTGC CTGTGCTCTT TGCGGTTGGT GGTCTTCTGT TGCTTTCCAA TGCCTCCTGT
    GTTGGGGGAC TCCTCTGGCG GAGAAGACTG AGGCGCCTTG CTGAGGAGAT CTCAGAGAAG
    ACAGAGGCAG GGTCGGAGGA GGATCGAATC AGGAATGAAT ATGAGGAGAG TCAGTGGACT
    GGGGACCGGG ACACAAGAAG CTCCACGGTT AGCACAGCAG AAGTGGACCC ACACTACTAC
    TCCATGAGGG ACTTCAGTCC CCAGCTTCCC CCAACACTGG AAGAGGTGTC ATATCGCCAA
    GCCTTCACAG GTATTGAAGA TGAGGACATG GCCTTCCCCG GACACCTGTA TGACGAGGTG
    GAGAGAGTCT ATGGCCCACC TGGGGTCTGG GGACCCCTCT ATGATGAAGT ACAAATGGAC
    CCCTATGACC TTCGCTGGCC TGAGGTCAAA TATGAGGACC CAAGGGGAAT CTACGACCAG
    GTGGCGGCAG ACATGGATGC TGGGGAGCCT GGTTCTCTGC CCTTTGAGCT GAGGGGACAT
    CTGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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