Rrad cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Rrad
Entrez Gene ID	56437
Full Name	Ras-related associated with diabetes
Synonyms	REM3,Rad
General protein information	Preferred Names Ras-related associated with diabetes Names GTP-binding protein RAD Ras-like GTP-binding protein rad
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_019662.3	NP_062636.2	GTP-binding protein RAD
NM_019662.2	NP_062636.2	GTP-binding protein RAD
XM_006531206.2	XP_006531269.1	GTP-binding protein RAD isoform X1
XM_006531206.1	XP_006531269.1	GTP-binding protein RAD isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rrad	NP_062636.2
Rattus norvegicus (Norway rat)	Rrad	NP_445790.2
Bos taurus (cattle)	RRAD	NP_001039378.1
Xenopus tropicalis (tropical clawed frog)	rrad	NP_001016726.1
Pan troglodytes (chimpanzee)	RRAD	XP_001143391.2
	RRAD	XP_001086160.1
Canis lupus familiaris (dog)	RRAD	XP_854048.1
Homo sapiens (human)	RRAD	NP_001122322.1
Gallus gallus (chicken)	RRAD	NP_001264535.1
Danio rerio (zebrafish)	rrad	NP_956092.1

Gene Ontology
Bibliography

Related articles in PubMed

Rad GTPase is essential for the regulation of bone density and bone marrow adipose tissue in mice.
Withers CN, Brown DM, Byiringiro I, Allen MR, Condon KW, Satin J, Andres DA
Bone103270-280(2017 Oct)

Rad-deletion Phenocopies Tonic Sympathetic Stimulation of the Heart.
Levitan BM, Manning JR, Withers CN, Smith JD, Shaw RM, Andres DA, Sorrell VL, Satin J
Journal of cardiovascular translational research9(5-6)432-444(2016 Dec)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Ray JP, Staron MM, Shyer JA, Ho PC, Marshall HD, Gray SM, Laidlaw BJ, Araki K, Ahmed R, Kaech SM, Craft J
Immunity43(4)690-702(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rad reduction provides a stable inotropic response rooted in sarcomere level function in hearts of knockout mice.
Title: Rad-deletion Phenocopies Tonic Sympathetic Stimulation of the Heart.

The Rad(-/-) phenotype is marked by enhanced systolic and diastolic function, which is consistent with a model that does not progress into heart failure.
Title: Loss of Rad-GTPase produces a novel adaptive cardiac phenotype resistant to systolic decline with aging.

A dominant-negative Rad mutation causes arrhythmogenesis via PKA-mediated phosphorylation of RYR2 activity in the heart.
Title: Suppression of Rad leads to arrhythmogenesis via PKA-mediated phosphorylation of ryanodine receptor activity in the heart.

Rad, Gem and Rem all reduce depolarization-dependent Ca2+ entry in developing myotubes.
Title: RGK protein-mediated impairment of slow depolarization- dependent Ca2+ entry into developing myotubes.

Depletion of Rad GTPase increases L-type calcium channel current leading to increased cardiac contraction, without stimulating cardiac hypertrophy.
Title: Rad GTPase deletion increases L-type calcium channel current leading to increased cardiac contraction.

The following Rrad gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rrad cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11505	NM_019662.3 Latest version!	Mus musculus Ras-related associated with diabetes (Rrad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu11505	XM_006531206.2 Latest version!	Mus musculus Ras-related associated with diabetes (Rrad), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu11505	NM_019662.2	Mus musculus Ras-related associated with diabetes (Rrad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu11505	XM_006531206.1	Mus musculus Ras-related associated with diabetes (Rrad), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rrad ( NM_019662.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062636.2 Mus musculus Ras-related associated with diabetes (Rrad), mRNA.

Price & Availability↑

CloneID	OMu11505
Clone ID Related Accession (Same CDS sequence)	NM_019662.3 , NM_019662.2 , XM_006531206.2 , XM_006531206.1
Accession Version	NM_019662.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	GTP-binding protein RAD
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK087473.1, AK159550.1 and AI894172.1. On Mar 4, 2019 this sequence version replaced NM_019662.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057138.1, SRR7345562.1088878.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849389, SAMN01164137 [ECO:0000348] ##Evidence-Data-END##

ATGACCCTGA ATGGTGGCAG CGGAGCAAGC GGGAGCCGTG GTGCGGGCCG GGAGCGCGAC 
CGCCGTCGAG GCAGCACCCC ATGGGGCCCG GCACCCCCAC TGCACCGCAG GAGCATGCCG 
GTGGACGAGC GAGACCTTCA GGCGGCGCTA GCCCCGGGCT CCCTGGCAAC GACTGCCGCT 
GGAACTCGGA CACAGGGCCA GAGGCTGGAC TGGCCCGAAG GCTCCTCCGA CTCGCTCAGT 
TCTGGAGGCA GTGGGTCAGA GGAGGGCGTT TACAAGGTGC TGCTGCTCGG GGCGCCTGGT 
GTGGGCAAAA GCGCTCTGGC GCGCATCTTT GGTGGTATAG AAGACGGACC TGAAGCAGAA 
GCAGCAGGGC ACACATATGA CCGTTCTATC ACTGTGGATG GAGAAGAAGC GTCACTATTG 
GTCTATGACA TTTGGGAACA GGATGGGGGC TGCTGGCTGC CTGGCCACTG CATGGCCATG 
GGGGATGCAT ACGTCATCGT GTACTCCATC ACGGACAAGG GCAGCTTTGA GAAAGCCTCA 
GAACTCCGGG TCCAGCTGCG GCGGGCCCGG CAGACAGACG ACGTGCCCAT CATTCTCGTG 
GGCAACAAGA GTGACCTGGT GCGCTCTCGT GAGGTCTCTG TGGATGAGGG CCGGGCCTGC 
GCAGTGGTCT TCGACTGCAA GTTCATCGAG ACATCAGCAG CACTGCATCA CAATGTCCAG 
GCACTATTCG AGGGGGTTGT GCGCCAGATA CGCCTGCGCA GGGACAGCAA AGAAGATAAC 
GCCCGGCGGC AAGCTGGCAC TCGGCGACGG GAGAGCCTTG GCAAGAAGGC CAAACGCTTT 
CTGGGCCGCA TCGTGGCTCG CAACAGCCGC AAGATGGCCT TCCGCGCCAA GTCCAAGTCC 
TGCCATGACC TCTCTGTTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062636.2
CDS	235..1158
Translation	MTLNGGSGASGSRGAGRERDRRRGSTPWGPAPPLHRRSMPVDERDLQAALAPGSLATTAAGTRTQGQRLDWPEGSSDSLSSGGSGSEEGVYKVLLLGAPGVGKSALARIFGGIEDGPEAEAAGHTYDRSITVDGEEASLLVYDIWEQDGGCWLPGHCMAMGDAYVIVYSITDKGSFEKASELRVQLRRARQTDDVPIILVGNKSDLVRSREVSVDEGRACAVVFDCKFIETSAALHHNVQALFEGVVRQIRLRRDSKEDNARRQAGTRRRESLGKKAKRFLGRIVARNSRKMAFRAKSKSCHDLSVL

Target ORF information:

RefSeq Version	NM_019662.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ras-related associated with diabetes (Rrad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019662.3

ORF Insert Sequence:

ATGACCCTGA ATGGTGGCAG CGGAGCAAGC GGGAGCCGTG GTGCGGGCCG GGAGCGCGAC 
CGCCGTCGAG GCAGCACCCC ATGGGGCCCG GCACCCCCAC TGCACCGCAG GAGCATGCCG 
GTGGACGAGC GAGACCTTCA GGCGGCGCTA GCCCCGGGCT CCCTGGCAAC GACTGCCGCT 
GGAACTCGGA CACAGGGCCA GAGGCTGGAC TGGCCCGAAG GCTCCTCCGA CTCGCTCAGT 
TCTGGAGGCA GTGGGTCAGA GGAGGGCGTT TACAAGGTGC TGCTGCTCGG GGCGCCTGGT 
GTGGGCAAAA GCGCTCTGGC GCGCATCTTT GGTGGTATAG AAGACGGACC TGAAGCAGAA 
GCAGCAGGGC ACACATATGA CCGTTCTATC ACTGTGGATG GAGAAGAAGC GTCACTATTG 
GTCTATGACA TTTGGGAACA GGATGGGGGC TGCTGGCTGC CTGGCCACTG CATGGCCATG 
GGGGATGCAT ACGTCATCGT GTACTCCATC ACGGACAAGG GCAGCTTTGA GAAAGCCTCA 
GAACTCCGGG TCCAGCTGCG GCGGGCCCGG CAGACAGACG ACGTGCCCAT CATTCTCGTG 
GGCAACAAGA GTGACCTGGT GCGCTCTCGT GAGGTCTCTG TGGATGAGGG CCGGGCCTGC 
GCAGTGGTCT TCGACTGCAA GTTCATCGAG ACATCAGCAG CACTGCATCA CAATGTCCAG 
GCACTATTCG AGGGGGTTGT GCGCCAGATA CGCCTGCGCA GGGACAGCAA AGAAGATAAC 
GCCCGGCGGC AAGCTGGCAC TCGGCGACGG GAGAGCCTTG GCAAGAAGGC CAAACGCTTT 
CTGGGCCGCA TCGTGGCTCG CAACAGCCGC AAGATGGCCT TCCGCGCCAA GTCCAAGTCC 
TGCCATGACC TCTCTGTTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rrad ( NM_019662.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062636.2 Mus musculus Ras-related associated with diabetes (Rrad), mRNA.

Price & Availability↑

CloneID	OMu11505
Clone ID Related Accession (Same CDS sequence)	NM_019662.3 , NM_019662.2 , XM_006531206.2 , XM_006531206.1
Accession Version	NM_019662.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-09
Organism	Mus musculus(house mouse)
Product	GTP-binding protein RAD
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY166399.1, AK159550.1 and AI481364.1. On Sep 7, 2006 this sequence version replaced NM_019662.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK077271.1, BC057138.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849389, SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCTGA ATGGTGGCAG CGGAGCAAGC GGGAGCCGTG GTGCGGGCCG GGAGCGCGAC 
CGCCGTCGAG GCAGCACCCC ATGGGGCCCG GCACCCCCAC TGCACCGCAG GAGCATGCCG 
GTGGACGAGC GAGACCTTCA GGCGGCGCTA GCCCCGGGCT CCCTGGCAAC GACTGCCGCT 
GGAACTCGGA CACAGGGCCA GAGGCTGGAC TGGCCCGAAG GCTCCTCCGA CTCGCTCAGT 
TCTGGAGGCA GTGGGTCAGA GGAGGGCGTT TACAAGGTGC TGCTGCTCGG GGCGCCTGGT 
GTGGGCAAAA GCGCTCTGGC GCGCATCTTT GGTGGTATAG AAGACGGACC TGAAGCAGAA 
GCAGCAGGGC ACACATATGA CCGTTCTATC ACTGTGGATG GAGAAGAAGC GTCACTATTG 
GTCTATGACA TTTGGGAACA GGATGGGGGC TGCTGGCTGC CTGGCCACTG CATGGCCATG 
GGGGATGCAT ACGTCATCGT GTACTCCATC ACGGACAAGG GCAGCTTTGA GAAAGCCTCA 
GAACTCCGGG TCCAGCTGCG GCGGGCCCGG CAGACAGACG ACGTGCCCAT CATTCTCGTG 
GGCAACAAGA GTGACCTGGT GCGCTCTCGT GAGGTCTCTG TGGATGAGGG CCGGGCCTGC 
GCAGTGGTCT TCGACTGCAA GTTCATCGAG ACATCAGCAG CACTGCATCA CAATGTCCAG 
GCACTATTCG AGGGGGTTGT GCGCCAGATA CGCCTGCGCA GGGACAGCAA AGAAGATAAC 
GCCCGGCGGC AAGCTGGCAC TCGGCGACGG GAGAGCCTTG GCAAGAAGGC CAAACGCTTT 
CTGGGCCGCA TCGTGGCTCG CAACAGCCGC AAGATGGCCT TCCGCGCCAA GTCCAAGTCC 
TGCCATGACC TCTCTGTTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062636.2
CDS	267..1190
Translation	MTLNGGSGASGSRGAGRERDRRRGSTPWGPAPPLHRRSMPVDERDLQAALAPGSLATTAAGTRTQGQRLDWPEGSSDSLSSGGSGSEEGVYKVLLLGAPGVGKSALARIFGGIEDGPEAEAAGHTYDRSITVDGEEASLLVYDIWEQDGGCWLPGHCMAMGDAYVIVYSITDKGSFEKASELRVQLRRARQTDDVPIILVGNKSDLVRSREVSVDEGRACAVVFDCKFIETSAALHHNVQALFEGVVRQIRLRRDSKEDNARRQAGTRRRESLGKKAKRFLGRIVARNSRKMAFRAKSKSCHDLSVL

Target ORF information:

RefSeq Version	NM_019662.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ras-related associated with diabetes (Rrad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019662.2

ORF Insert Sequence:

ATGACCCTGA ATGGTGGCAG CGGAGCAAGC GGGAGCCGTG GTGCGGGCCG GGAGCGCGAC 
CGCCGTCGAG GCAGCACCCC ATGGGGCCCG GCACCCCCAC TGCACCGCAG GAGCATGCCG 
GTGGACGAGC GAGACCTTCA GGCGGCGCTA GCCCCGGGCT CCCTGGCAAC GACTGCCGCT 
GGAACTCGGA CACAGGGCCA GAGGCTGGAC TGGCCCGAAG GCTCCTCCGA CTCGCTCAGT 
TCTGGAGGCA GTGGGTCAGA GGAGGGCGTT TACAAGGTGC TGCTGCTCGG GGCGCCTGGT 
GTGGGCAAAA GCGCTCTGGC GCGCATCTTT GGTGGTATAG AAGACGGACC TGAAGCAGAA 
GCAGCAGGGC ACACATATGA CCGTTCTATC ACTGTGGATG GAGAAGAAGC GTCACTATTG 
GTCTATGACA TTTGGGAACA GGATGGGGGC TGCTGGCTGC CTGGCCACTG CATGGCCATG 
GGGGATGCAT ACGTCATCGT GTACTCCATC ACGGACAAGG GCAGCTTTGA GAAAGCCTCA 
GAACTCCGGG TCCAGCTGCG GCGGGCCCGG CAGACAGACG ACGTGCCCAT CATTCTCGTG 
GGCAACAAGA GTGACCTGGT GCGCTCTCGT GAGGTCTCTG TGGATGAGGG CCGGGCCTGC 
GCAGTGGTCT TCGACTGCAA GTTCATCGAG ACATCAGCAG CACTGCATCA CAATGTCCAG 
GCACTATTCG AGGGGGTTGT GCGCCAGATA CGCCTGCGCA GGGACAGCAA AGAAGATAAC 
GCCCGGCGGC AAGCTGGCAC TCGGCGACGG GAGAGCCTTG GCAAGAAGGC CAAACGCTTT 
CTGGGCCGCA TCGTGGCTCG CAACAGCCGC AAGATGGCCT TCCGCGCCAA GTCCAAGTCC 
TGCCATGACC TCTCTGTTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rrad ( XM_006531206.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531269.1 Mus musculus Ras-related associated with diabetes (Rrad), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu11505
Clone ID Related Accession (Same CDS sequence)	NM_019662.3 , NM_019662.2 , XM_006531206.2 , XM_006531206.1
Accession Version	XM_006531206.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	GTP-binding protein RAD isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTGA ATGGTGGCAG CGGAGCAAGC GGGAGCCGTG GTGCGGGCCG GGAGCGCGAC 
CGCCGTCGAG GCAGCACCCC ATGGGGCCCG GCACCCCCAC TGCACCGCAG GAGCATGCCG 
GTGGACGAGC GAGACCTTCA GGCGGCGCTA GCCCCGGGCT CCCTGGCAAC GACTGCCGCT 
GGAACTCGGA CACAGGGCCA GAGGCTGGAC TGGCCCGAAG GCTCCTCCGA CTCGCTCAGT 
TCTGGAGGCA GTGGGTCAGA GGAGGGCGTT TACAAGGTGC TGCTGCTCGG GGCGCCTGGT 
GTGGGCAAAA GCGCTCTGGC GCGCATCTTT GGTGGTATAG AAGACGGACC TGAAGCAGAA 
GCAGCAGGGC ACACATATGA CCGTTCTATC ACTGTGGATG GAGAAGAAGC GTCACTATTG 
GTCTATGACA TTTGGGAACA GGATGGGGGC TGCTGGCTGC CTGGCCACTG CATGGCCATG 
GGGGATGCAT ACGTCATCGT GTACTCCATC ACGGACAAGG GCAGCTTTGA GAAAGCCTCA 
GAACTCCGGG TCCAGCTGCG GCGGGCCCGG CAGACAGACG ACGTGCCCAT CATTCTCGTG 
GGCAACAAGA GTGACCTGGT GCGCTCTCGT GAGGTCTCTG TGGATGAGGG CCGGGCCTGC 
GCAGTGGTCT TCGACTGCAA GTTCATCGAG ACATCAGCAG CACTGCATCA CAATGTCCAG 
GCACTATTCG AGGGGGTTGT GCGCCAGATA CGCCTGCGCA GGGACAGCAA AGAAGATAAC 
GCCCGGCGGC AAGCTGGCAC TCGGCGACGG GAGAGCCTTG GCAAGAAGGC CAAACGCTTT 
CTGGGCCGCA TCGTGGCTCG CAACAGCCGC AAGATGGCCT TCCGCGCCAA GTCCAAGTCC 
TGCCATGACC TCTCTGTTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531269.1
CDS	261..1184
Translation	MTLNGGSGASGSRGAGRERDRRRGSTPWGPAPPLHRRSMPVDERDLQAALAPGSLATTAAGTRTQGQRLDWPEGSSDSLSSGGSGSEEGVYKVLLLGAPGVGKSALARIFGGIEDGPEAEAAGHTYDRSITVDGEEASLLVYDIWEQDGGCWLPGHCMAMGDAYVIVYSITDKGSFEKASELRVQLRRARQTDDVPIILVGNKSDLVRSREVSVDEGRACAVVFDCKFIETSAALHHNVQALFEGVVRQIRLRRDSKEDNARRQAGTRRRESLGKKAKRFLGRIVARNSRKMAFRAKSKSCHDLSVL

Target ORF information:

RefSeq Version	XM_006531206.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ras-related associated with diabetes (Rrad), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531206.1

ORF Insert Sequence:

ATGACCCTGA ATGGTGGCAG CGGAGCAAGC GGGAGCCGTG GTGCGGGCCG GGAGCGCGAC 
CGCCGTCGAG GCAGCACCCC ATGGGGCCCG GCACCCCCAC TGCACCGCAG GAGCATGCCG 
GTGGACGAGC GAGACCTTCA GGCGGCGCTA GCCCCGGGCT CCCTGGCAAC GACTGCCGCT 
GGAACTCGGA CACAGGGCCA GAGGCTGGAC TGGCCCGAAG GCTCCTCCGA CTCGCTCAGT 
TCTGGAGGCA GTGGGTCAGA GGAGGGCGTT TACAAGGTGC TGCTGCTCGG GGCGCCTGGT 
GTGGGCAAAA GCGCTCTGGC GCGCATCTTT GGTGGTATAG AAGACGGACC TGAAGCAGAA 
GCAGCAGGGC ACACATATGA CCGTTCTATC ACTGTGGATG GAGAAGAAGC GTCACTATTG 
GTCTATGACA TTTGGGAACA GGATGGGGGC TGCTGGCTGC CTGGCCACTG CATGGCCATG 
GGGGATGCAT ACGTCATCGT GTACTCCATC ACGGACAAGG GCAGCTTTGA GAAAGCCTCA 
GAACTCCGGG TCCAGCTGCG GCGGGCCCGG CAGACAGACG ACGTGCCCAT CATTCTCGTG 
GGCAACAAGA GTGACCTGGT GCGCTCTCGT GAGGTCTCTG TGGATGAGGG CCGGGCCTGC 
GCAGTGGTCT TCGACTGCAA GTTCATCGAG ACATCAGCAG CACTGCATCA CAATGTCCAG 
GCACTATTCG AGGGGGTTGT GCGCCAGATA CGCCTGCGCA GGGACAGCAA AGAAGATAAC 
GCCCGGCGGC AAGCTGGCAC TCGGCGACGG GAGAGCCTTG GCAAGAAGGC CAAACGCTTT 
CTGGGCCGCA TCGTGGCTCG CAACAGCCGC AAGATGGCCT TCCGCGCCAA GTCCAAGTCC 
TGCCATGACC TCTCTGTTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rrad ( XM_006531206.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531269.1 Mus musculus Ras-related associated with diabetes (Rrad), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu11505
Clone ID Related Accession (Same CDS sequence)	NM_019662.3 , NM_019662.2 , XM_006531206.2 , XM_006531206.1
Accession Version	XM_006531206.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GTP-binding protein RAD isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTGA ATGGTGGCAG CGGAGCAAGC GGGAGCCGTG GTGCGGGCCG GGAGCGCGAC 
CGCCGTCGAG GCAGCACCCC ATGGGGCCCG GCACCCCCAC TGCACCGCAG GAGCATGCCG 
GTGGACGAGC GAGACCTTCA GGCGGCGCTA GCCCCGGGCT CCCTGGCAAC GACTGCCGCT 
GGAACTCGGA CACAGGGCCA GAGGCTGGAC TGGCCCGAAG GCTCCTCCGA CTCGCTCAGT 
TCTGGAGGCA GTGGGTCAGA GGAGGGCGTT TACAAGGTGC TGCTGCTCGG GGCGCCTGGT 
GTGGGCAAAA GCGCTCTGGC GCGCATCTTT GGTGGTATAG AAGACGGACC TGAAGCAGAA 
GCAGCAGGGC ACACATATGA CCGTTCTATC ACTGTGGATG GAGAAGAAGC GTCACTATTG 
GTCTATGACA TTTGGGAACA GGATGGGGGC TGCTGGCTGC CTGGCCACTG CATGGCCATG 
GGGGATGCAT ACGTCATCGT GTACTCCATC ACGGACAAGG GCAGCTTTGA GAAAGCCTCA 
GAACTCCGGG TCCAGCTGCG GCGGGCCCGG CAGACAGACG ACGTGCCCAT CATTCTCGTG 
GGCAACAAGA GTGACCTGGT GCGCTCTCGT GAGGTCTCTG TGGATGAGGG CCGGGCCTGC 
GCAGTGGTCT TCGACTGCAA GTTCATCGAG ACATCAGCAG CACTGCATCA CAATGTCCAG 
GCACTATTCG AGGGGGTTGT GCGCCAGATA CGCCTGCGCA GGGACAGCAA AGAAGATAAC 
GCCCGGCGGC AAGCTGGCAC TCGGCGACGG GAGAGCCTTG GCAAGAAGGC CAAACGCTTT 
CTGGGCCGCA TCGTGGCTCG CAACAGCCGC AAGATGGCCT TCCGCGCCAA GTCCAAGTCC 
TGCCATGACC TCTCTGTTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531269.1
CDS	244..1167
Translation	MTLNGGSGASGSRGAGRERDRRRGSTPWGPAPPLHRRSMPVDERDLQAALAPGSLATTAAGTRTQGQRLDWPEGSSDSLSSGGSGSEEGVYKVLLLGAPGVGKSALARIFGGIEDGPEAEAAGHTYDRSITVDGEEASLLVYDIWEQDGGCWLPGHCMAMGDAYVIVYSITDKGSFEKASELRVQLRRARQTDDVPIILVGNKSDLVRSREVSVDEGRACAVVFDCKFIETSAALHHNVQALFEGVVRQIRLRRDSKEDNARRQAGTRRRESLGKKAKRFLGRIVARNSRKMAFRAKSKSCHDLSVL

Target ORF information:

RefSeq Version	XM_006531206.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ras-related associated with diabetes (Rrad), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531206.2

ORF Insert Sequence:

ATGACCCTGA ATGGTGGCAG CGGAGCAAGC GGGAGCCGTG GTGCGGGCCG GGAGCGCGAC 
CGCCGTCGAG GCAGCACCCC ATGGGGCCCG GCACCCCCAC TGCACCGCAG GAGCATGCCG 
GTGGACGAGC GAGACCTTCA GGCGGCGCTA GCCCCGGGCT CCCTGGCAAC GACTGCCGCT 
GGAACTCGGA CACAGGGCCA GAGGCTGGAC TGGCCCGAAG GCTCCTCCGA CTCGCTCAGT 
TCTGGAGGCA GTGGGTCAGA GGAGGGCGTT TACAAGGTGC TGCTGCTCGG GGCGCCTGGT 
GTGGGCAAAA GCGCTCTGGC GCGCATCTTT GGTGGTATAG AAGACGGACC TGAAGCAGAA 
GCAGCAGGGC ACACATATGA CCGTTCTATC ACTGTGGATG GAGAAGAAGC GTCACTATTG 
GTCTATGACA TTTGGGAACA GGATGGGGGC TGCTGGCTGC CTGGCCACTG CATGGCCATG 
GGGGATGCAT ACGTCATCGT GTACTCCATC ACGGACAAGG GCAGCTTTGA GAAAGCCTCA 
GAACTCCGGG TCCAGCTGCG GCGGGCCCGG CAGACAGACG ACGTGCCCAT CATTCTCGTG 
GGCAACAAGA GTGACCTGGT GCGCTCTCGT GAGGTCTCTG TGGATGAGGG CCGGGCCTGC 
GCAGTGGTCT TCGACTGCAA GTTCATCGAG ACATCAGCAG CACTGCATCA CAATGTCCAG 
GCACTATTCG AGGGGGTTGT GCGCCAGATA CGCCTGCGCA GGGACAGCAA AGAAGATAAC 
GCCCGGCGGC AAGCTGGCAC TCGGCGACGG GAGAGCCTTG GCAAGAAGGC CAAACGCTTT 
CTGGGCCGCA TCGTGGCTCG CAACAGCCGC AAGATGGCCT TCCGCGCCAA GTCCAAGTCC 
TGCCATGACC TCTCTGTTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rad GTPase is essential for the regulation of bone density and bone marrow adipose tissue in mice.
Bone103270-280(2017 Oct)
Withers CN,Brown DM,Byiringiro I,Allen MR,Condon KW,Satin J,Andres DA

Rad-deletion Phenocopies Tonic Sympathetic Stimulation of the Heart.
Journal of cardiovascular translational research9(5-6)432-444(2016 Dec)
Levitan BM,Manning JR,Withers CN,Smith JD,Shaw RM,Andres DA,Sorrell VL,Satin J

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Immunity43(4)690-702(2015 Oct)
Ray JP,Staron MM,Shyer JA,Ho PC,Marshall HD,Gray SM,Laidlaw BJ,Araki K,Ahmed R,Kaech SM,Craft J

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Rrad cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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