Gene Symbol | Fhl5 |
Entrez Gene ID | 57756 |
Full Name | four and a half LIM domains 5 |
Synonyms | 1700027G07Rik,Act |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
ORF » Species Summary » Mus musculus » Fhl5 cDNA ORF clone
gRNAs
Gene Symbol | Fhl5 |
Entrez Gene ID | 57756 |
Full Name | four and a half LIM domains 5 |
Synonyms | 1700027G07Rik,Act |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
mRNA | Protein | Name |
---|---|---|
XM_011250080.2 | XP_011248382.1 | four and a half LIM domains protein 5 isoform X2 |
XM_011250080.1 | XP_011248382.1 | four and a half LIM domains protein 5 isoform X1 |
XM_006538125.3 | XP_006538188.1 | four and a half LIM domains protein 5 isoform X1 |
XM_006538125.4 | XP_006538188.1 | four and a half LIM domains protein 5 isoform X1 |
NM_001355498.1 | NP_001342427.1 | four and a half LIM domains protein 5 |
NM_021318.3 | NP_067293.1 | four and a half LIM domains protein 5 |
NM_021318.4 | NP_067293.1 | four and a half LIM domains protein 5 |
Rattus norvegicus (Norway rat) | Fhl5 | NP_001013106.1 |
Gallus gallus (chicken) | FHL5 | NP_001264652.1 |
FHL5 | XP_001100773.2 | |
Canis lupus familiaris (dog) | FHL5 | XP_532237.2 |
Pan troglodytes (chimpanzee) | FHL5 | NP_001233299.1 |
Bos taurus (cattle) | FHL5 | NP_001069847.1 |
Homo sapiens (human) | FHL5 | NP_001164278.1 |
Mus musculus (house mouse) | Fhl5 | NP_067293.1 |
Xenopus tropicalis (tropical clawed frog) | fhl5 | NP_001116957.1 |
TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice.
Yabuta Y, Ohta H, Abe T, Kurimoto K, Chuma S, Saitou M
The Journal of cell biology192(5)781-95(2011 Mar)
Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis.
Wu H, Chen Y, Miao S, Zhang C, Zong S, Koide SS, Wang L
FEBS letters584(13)2807-15(2010 Jul)
A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)
Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression.
Lardenois A, Chalmel F, Demougin P, Kotaja N, Sassone-Corsi P, Primig M
Reproductive biology and endocrinology : RB&E7133(2009 Nov)
GUDMAP: the genitourinary developmental molecular anatomy project.
McMahon AP, Aronow BJ, Davidson DR, Davies JA, Gaido KW, Grimmond S, Lessard JL, Little MH, Potter SS, Wilder EL, Zhang P,
Journal of the American Society of Nephrology : JASN19(4)667-71(2008 Apr)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Results indicate that SPAG8 acts as a regulator of ACT and plays an important role in CREM-ACT-mediated gene transcription during spermatogenesis.
Title: Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis.
Fhl5 is not essential for testicular gene expression to a level detected by microarray-based measurements.
Title: Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression.
Results indicate that numerous postmeiotic genes under CREM (cAMP-responsive element modulator) control require the coactivator function of ACT (activator of CREM) in testis.
Title: Abnormal sperm in mice with targeted deletion of the act (activator of cAMP-responsive element modulator in testis) gene.
ACT selectively associates with KIF17b, a kinesin highly expressed in male germ cells; The ACT-KIF17b interaction is restricted to specific stages of spermatogenesis and directly determines the intracellular localization of ACT
Title: CREM-dependent transcription in male germ cells controlled by a kinesin.
The following Fhl5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fhl5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OMu33069 | |
Clone ID Related Accession (Same CDS sequence) | XM_006538125.3 , XM_006538125.4 | |
Accession Version | XM_006538125.3 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | four and a half LIM domains protein 5 isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538125.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGAATTGC CTCTCTGGGA TAAAACTGAA ATGACAAGTA GTCAATTTGA TTGTCAATAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006538188.1 |
CDS | 387..1271 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGAATTGC CTCTCTGGGA TAAAACTGAA ATGACAAGTA GTCAATTTGA TTGTCAATAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu12168 | |
Clone ID Related Accession (Same CDS sequence) | XM_011250080.2 , XM_011250080.1 , NM_001355498.1 , NM_021318.3 , NM_021318.4 | |
Accession Version | NM_001355498.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-11-15 | |
Organism | Mus musculus(house mouse) | |
Product | four and a half LIM domains protein 5 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL935267.13, AK006414.1 and AV205329.1. On Sep 19, 2017 this sequence version replaced XM_011250080.2. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.192388.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## |
1 | ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001342427.1 |
CDS | 77..931 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu12168 | |
Clone ID Related Accession (Same CDS sequence) | XM_011250080.2 , XM_011250080.1 , NM_001355498.1 , NM_021318.3 , NM_021318.4 | |
Accession Version | NM_021318.3 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2015-02-14 | |
Organism | Mus musculus(House mouse) | |
Product | four and a half LIM domains protein 5 | |
Comment | PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC048473.1. On Apr 5, 2007 this sequence version replaced NM_021318.2. ##Evidence-Data-START## Transcript exon combination :: BC048473.1, AK006414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## |
1 | ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_067293.1 |
CDS | 182..1036 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu12168 | |
Clone ID Related Accession (Same CDS sequence) | XM_011250080.2 , XM_011250080.1 , NM_001355498.1 , NM_021318.3 , NM_021318.4 | |
Accession Version | NM_021318.4 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-11-15 | |
Organism | Mus musculus(house mouse) | |
Product | four and a half LIM domains protein 5 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL935267.13, AK006414.1 and AV205329.1. On Sep 19, 2017 this sequence version replaced NM_021318.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC048473.1, AK006414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## |
1 | ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_067293.1 |
CDS | 204..1058 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu12168 | |
Clone ID Related Accession (Same CDS sequence) | XM_011250080.2 , XM_011250080.1 , NM_001355498.1 , NM_021318.3 , NM_021318.4 | |
Accession Version | XM_011250080.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(House mouse) | |
Product | four and a half LIM domains protein 5 isoform X2 | |
Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_011248382.1 |
CDS | 101..955 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu12168 | |
Clone ID Related Accession (Same CDS sequence) | XM_011250080.2 , XM_011250080.1 , NM_001355498.1 , NM_021318.3 , NM_021318.4 | |
Accession Version | XM_011250080.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2015-02-08 | |
Organism | Mus musculus(House mouse) | |
Product | four and a half LIM domains protein 5 isoform X1 | |
Comment |
1 | ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_011248382.1 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGACAAGTA GTCAATTTGA TTGTCAATAC TGCACTTCAT CCCTGATTGG GAAGAAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu33069 | |
Clone ID Related Accession (Same CDS sequence) | XM_006538125.3 , XM_006538125.4 | |
Accession Version | XM_006538125.4 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | four and a half LIM domains protein 5 isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538125.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGAATTGC CTCTCTGGGA TAAAACTGAA ATGACAAGTA GTCAATTTGA TTGTCAATAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006538188.1 |
CDS | 377..1261 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGAATTGC CTCTCTGGGA TAAAACTGAA ATGACAAGTA GTCAATTTGA TTGTCAATAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice. |
Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis. |
A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58. |
Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression. |
GUDMAP: the genitourinary developmental molecular anatomy project. |