Nelfb cDNA ORF clone, Mus musculus(house mouse)
Gene Symbol | Nelfb |
Entrez Gene ID | 58202 |
Full Name | negative elongation factor complex member B |
Synonyms | A730008L03Rik,AB041607,AI663983,Cobra1,Nelf-b |
General protein information |
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Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome | |
Summary | This gene encodes subunit B of a metazoan-specific, four-subunit protein complex that regulates promoter-proximal pausing of RNA polymerase II. RNA polymerase II pausing is thought to be important for coordination of gene transcription during embryonic development and stress responses. Consistently, disruption of this gene in mouse causes inner cell mass deficiency and embryonic lethality. In addition, this gene is required for maintenance of mouse embryonic stem cells by preventing expression of developmental genes. In adult mice, conditional deletion of this gene results in cardiomyopathy and impaired response to cardiac stress. Multiple protein isoforms are encoded through the use of a non-AUG (CUG) initiation codon and an alternative downstream AUG initiation codon. In addition, alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]. |
mRNA and Protein(s)
mRNA | Protein | Name |
---|---|---|
NM_001310157.1 | NP_001297086.1 | negative elongation factor B isoform 2 |
NM_021393.3 | NP_067368.3 | negative elongation factor B isoform 1 |

COBRA1 | XP_001087434.1 | |
Rattus norvegicus (Norway rat) | Nelfb | NP_001101287.2 |
Anopheles gambiae (African malaria mosquito) | AgaP_AGAP012109 | XP_320419.3 |
Homo sapiens (human) | NELFB | NP_056271.2 |
Bos taurus (cattle) | NELFB | XP_002691742.3 |
Xenopus tropicalis (tropical clawed frog) | nelfb | NP_001011448.1 |
Mus musculus (house mouse) | Nelfb | NP_067368.2 |
Gallus gallus (chicken) | COBRA1 | NP_001006202.1 |
Pan troglodytes (chimpanzee) | COBRA1 | XP_001136264.3 |
Canis lupus familiaris (dog) | NELFB | XP_548351.2 |
Danio rerio (zebrafish) | nelfb | NP_956462.1 |
Drosophila melanogaster (fruit fly) | NELF-B | NP_572402.1 |

Related articles in PubMed
Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis.
Zhang X, Chiang HC, Wang Y, Zhang C, Smith S, Zhao X, Nair SJ, Michalek J, Jatoi I, Lautner M, Oliver B, Wang H, Petit A, Soler T, Brunet J, Mateo F, Angel Pujana M, Poggi E, Chaldekas K, Isaacs C, Peshkin BN, Ochoa O, Chedin F, Theoharis C, Sun LZ, Curiel TJ, Elledge R, Jin VX, Hu Y, Li R
Nature communications815908(2017 Jun)
Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.
Pan H, Zhao X, Zhang X, Abouelsoud M, Sun J, April C, Amleh A, Fan JB, Hu Y, Li R
PloS one10(5)e0127422(2015)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
This study uncovers a previously unappreciated, DNA repair-independent function of BRCA1 in antagonizing COBRA1-dependent transcription program during mammary gland development.
Title: Genetic suppression reveals DNA repair-independent antagonism between BRCA1 and COBRA1 in mammary gland development.
Human and mouse Nelfb encode proteins translated from a non-AUG (CUG) start codon. The majority of mouse tissues examined express the isoform translated from the CUG start codon, but a small number of tissues also express a shorter isoform of the same apparent size as the AUG-initiated isoform.
Title: Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.
Cobra1 Is Required for TrkC-Induced Apoptosis.
Title: The dependence receptor TrkC triggers mitochondria-dependent apoptosis upon Cobra-1 recruitment.
genetic and genomic studies provide new physiological and molecular insight into Nelf-mediated pol II pausing.
Title: Genetic and genomic analyses of RNA polymerase II-pausing factor in regulation of mammalian transcription and cell growth.
findings also indicate that Cobra1 helps maintain the undifferentiated state of mESCs by preventing unscheduled expression of developmental genes
Title: Mouse cofactor of BRCA1 (Cobra1) is required for early embryogenesis.
The following Nelfb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nelfb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OMu69773 | |
Clone ID Related Accession (Same CDS sequence) | NM_001310157.1 | |
Accession Version | NM_001310157.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP |
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Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-22 | |
Organism | Mus musculus(house mouse) | |
Product | negative elongation factor B isoform 2 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL732309.9, CJ100279.1, CX241076.1, AK173124.1 and AI843129.1. Transcript Variant: This variant (2) differs in the 5' UTR and uses alternate splice sites in the 5' coding region, which results in use of a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB041607.1, SRR1660817.230045.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGCCATCCC TACAACCAGT GGTGATGTGT GTTATGAAAC ACTTGCCCAA GGTTCCTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001297086.1 |
CDS | 831..2258 |
Translation |

Target ORF information:
Target ORF information:
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ORF Insert Sequence:
1 | ATGCCATCCC TACAACCAGT GGTGATGTGT GTTATGAAAC ACTTGCCCAA GGTTCCTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu69774 | |
Clone ID Related Accession (Same CDS sequence) | NM_021393.3 | |
Accession Version | NM_021393.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP |
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Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-22 | |
Organism | Mus musculus(house mouse) | |
Product | negative elongation factor B isoform 1 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL732309.9, CJ100279.1, AK173124.1 and AI843129.1. On Jul 3, 2015 this sequence version replaced NM_021393.2. Transcript Variant: This variant (1) represents the longer isoform (1), which is translated using a non-AUG (CUG) start codon. CCDS Note: The coding region has been updated to extend the protein N-terminus and to use a non-AUG (CUG) start codon, supported by data in PMID:26010750. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC004762.1, SRR1660813.236916.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 26010750 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. |
1 | CTGGAGGCGG CGGGCGAACG GGGCTTGGGG GGTCCTCGTG GCACCGTGGA GAGGGCTTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_067368.3 |
CDS | 143..2029 |
Translation |

Target ORF information:
Target ORF information:
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ORF Insert Sequence:
1 | CTGGAGGCGG CGGGCGAACG GGGCTTGGGG GGTCCTCGTG GCACCGTGGA GAGGGCTTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
![]() |
Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis. |
![]() |
Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B. |