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Cep85 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Cep85
Entrez Gene ID 70012
Full Name centrosomal protein 85
Synonyms 2410030J07Rik,AI173272,AI173504,Ccdc21
General protein information
Preferred Names

centrosomal protein 85

Names

centrosomal protein of 85 kDa
coiled-coil domain containing 21
coiled-coil domain-containing protein 21
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

4

4|4 D3

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_011250330.2 XP_011248632.1 centrosomal protein of 85 kDa isoform X1
XM_006539164.1 XP_006539227.1 centrosomal protein of 85 kDa isoform X3
NM_144527.3 NP_653110.3 centrosomal protein of 85 kDa
XM_011250331.2 XP_011248633.1 centrosomal protein of 85 kDa isoform X2

Pathways from BioSystems


Homologies


Homo sapiens (human) CEP85 NP_073615.2
Mus musculus (house mouse) Cep85 NP_653110.3
Rattus norvegicus (Norway rat) Cep85 NP_001073620.1
Gallus gallus (chicken) CEP85 XP_417697.2
Danio rerio (zebrafish) si:ch211-11i5.2 XP_690486.4
Pan troglodytes (chimpanzee) CEP85 XP_001142011.2
Canis lupus familiaris (dog) CEP85 XP_544483.3
CEP85 XP_001108741.2
Bos taurus (cattle) CEP85 XP_002685748.2

Gene Ontology


Bibliography

Related articles in PubMed

Characterization of Cep85 - a new antagonist of Nek2A that is involved in the regulation of centrosome disjunction.
Chen C, Tian F, Lu L, Wang Y, Xiao Z, Yu C, Yu X
Journal of cell science128(17)3290-303(2015 Sep)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Exchangeable gene trap using the Cre/mutated lox system.
Araki K, Imaizumi T, Sekimoto T, Yoshinobu K, Yoshimuta J, Akizuki M, Miura K, Araki M, Yamamura K
Cellular and molecular biology (Noisy-le-Grand, France)45(5)737-50(1999 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cep85 is a bona fide Nek2A-binding partner that surrounds the proximal ends of centrioles where it cooperates with PP1gamma (also known as PPP1CC) to antagonize Nek2A
Title: Characterization of Cep85 - a new antagonist of Nek2A that is involved in the regulation of centrosome disjunction.

The following Cep85 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cep85 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu21139 NM_144527.3
Latest version!		 Mus musculus centrosomal protein 85 (Cep85), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu21139 XM_011250330.2
Latest version!		 Mus musculus centrosomal protein 85 (Cep85), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu34423 XM_006539164.1
Latest version!		 Mus musculus centrosomal protein 85 (Cep85), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu70486 XM_011250331.2
Latest version!		 Mus musculus centrosomal protein 85 (Cep85), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu21139
Clone ID Related Accession (Same CDS sequence) XM_011250330.2 , NM_144527.3
Accession Version NM_144527.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-19
Organism Mus musculus(house mouse)
Product centrosomal protein of 85 kDa
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY250554.1, AK085545.1, AK141103.1, AV301648.2 and AW123366.1. On Nov 15, 2007 this sequence version replaced NM_144527.2. ##Evidence-Data-START## Transcript exon combination :: AK141103.1, AK030742.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCCATGC AAGAAAAATA CCCAAATGAC AGGTCTCATG CTACTTCACC AGGTTCCAAT 
GTGATTCAAA AAGGCAGTTC TCTGGGAACT GAGTGGCAGA CACCAGTTAT CTCAGAGACC 
TTTCGGAGCC GCTTCAGCCG CTGCTCAAGC ATTGCTGACA GTGGGGACAC AGCCATTGGG 
ACATCATGCT CAGACATTGC AGAGGATTTT TGCAGCTCAA GTGGCAGCCC TTCCTTCCAG 
CCAATCAAAA GCCACATAAC CATTCCAACA GCCCATGTGA TGCCTTCTAC TTTGGGGGCC 
TCTCCTGCCA AACCAAATTC TGCACCTTCC GGGCCTTCTT CCGCTAAGCT GCCCTTGTCA 
GGATTGACTG AAGGCGTGGG GATGACAAGA AATGGAGACT TCGGAGCTGT GAAGCGCTCT 
CCAGGCCTAG CAAGGGATTT TATGTACCTC CCTAGTGCTG CCGGGGAGAA TGGGAGTCAG 
CAGTCCTGGT TCCCAGCAGT GGGCCACGAG AGAGAGGGGG AGATGAGGAA GTTCGATGTT 
CCTAGCATGG AGTCTACCCT TAACCAGCCA GCCATGCTAG AGACGTTATA CTCAGATCCA 
CATTACCGAG CCCATTTCCC CAACCCAAGA CCTGATACAA ATAAGGATGT ATACAAAGTA 
TTGCCAGAAT CCAAGAAGGC ACCGGGCAGT GGTGCAGTAT TTGAGAGGAA CGGACCACAT 
GCTAGCAGTA GTGGGGTGCT CCCTTTGGGA CTCCAGCCTG CGCCTGGACT TTCCAAGTCA 
CTATCCTCTC AGGTGTGGCA ACCAAGTCCT GACCCTTGGC ATCCTGGAGA ACAATCCTGT 
GAACTCAGTA CTTGTCGACA GCAGTTGGAA TTGATCCGTT TACAGATGGA GCAAATGCAG 
CTTCAGAACG GAGCCATGTG TCACCATCCT GCTGCTTTCG CTCCATTACT GCCCACCCTA 
GAGCCAGCAC AGTGGCTCAG CATCCTGAAC AGTAACGAGC ATCTCCTGAA GGAGAAGGAG 
CTCCTCATTG ACAAGCAAAG GAAGCATATC TCTCAGCTGG AGCAGAAAGT GCGAGAGAGT 
GAACTGCAAG TCCACAGTGC CCTTTTGGGC CGCCCTGCCC CCTTTGGGGA TGTCTGCTTA 
TTGAGGCTAC AGGAGTTGCA GCGAGAGAAC ACTTTCTTAC GGGCACAGTT TGCACAGAAG 
ACAGAAGCCC TGAGCAAGGA GAAGATGGAG CTTGAAAAGA AACTCTCTGC ATCTGAAGTT 
GAAATTCAGC TCATTAGGGA GTCTCTAAAA GTGACACTAC AGAAGCATTC GGAGGAGGGG 
AAGAAACAGG AGGAAAGGGT CAAAGGTCGT GATAAACATA TCAATAATTT GAAAAAGAAA 
TGTCAGAAGG AATCAGAGCA GAACCGGGAG AAGCAGCAGC GTATTGAAAC CTTGGAGCGC 
TATCTAGCTG ACCTGCCCAC CCTAGAAGAC CATCAGAAAC AGACGGAGCA GCTTAAGGAC 
GCTGAATTAA AGAACACAGA ACTGCAAGAG AGAGTGGCTG AGCTGGAGAC TTTGCTGGAG 
GACACCCAGG CAACCTGCAG AGAGAAGGAG GTTCAGCTGG AAAGTCTGAG ACAAAGAGAA 
GCAGACCTCT CCTCTGCTAG ACATAGCTTT CAAGATAAAC AGTCTGTGGA GGAAGCCAAT 
GGAGAAAACC TCAGAGTGGA CATGGAATCC CAGCAGAAGG AATGTGACTC CCTCCGAAAG 
ATGGTGGAGA GGCAACAGCT GAAGATGGAA CAGCTGCACT CCCAAGTCCA GAGCCAAAAG 
CAAGAACTGG CACAAGAAGA GGGAATCAAC CAGGCACTGA GAGAGGAGGC CCAGCGGAGG 
GAGACGGCCC TGCAGCAGAT GCGCACAGCC GTGAAGGAGC TTTCAGTGCA AAACCAGGAC 
CTGATTGAGA AGAATCTGAC GCTGCAAGAG CACTTGCGCC AGGCCCAGCC AGGGTCCTCA 
TCTTCACCAG ACTCAGCCCA GCTGGCCTGT GAGCTGCACC AGGAACTGGC CAGTTGCCTT 
CAAGATCTGC AAGCTGTCTG CAGCATTGTG ACCCAGAGGG CCCAGGGCCA CAATCCTAAC 
CTCTCACTAC TCCTAGGCAT TCACTCCACC CAGCACCCAG GGACGCAGCT AGATCTACAA 
AAGCCAGATG TGATCAGGAG GAAACTAGAA GAGGTTCAAC AGCTGCGCCA TGACATTGAG 
GACTTAAGGA CCAGCCTGTC AGACAGATAT GCCCAGGACA TGGGAGAAAA CTGTGCCACA 
CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_653110.3
CDS160..2445
Translation

Target ORF information:

RefSeq Version NM_144527.3
Organism Mus musculus(house mouse)
Definition Mus musculus centrosomal protein 85 (Cep85), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_144527.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCCATGC AAGAAAAATA CCCAAATGAC AGGTCTCATG CTACTTCACC AGGTTCCAAT 
GTGATTCAAA AAGGCAGTTC TCTGGGAACT GAGTGGCAGA CACCAGTTAT CTCAGAGACC 
TTTCGGAGCC GCTTCAGCCG CTGCTCAAGC ATTGCTGACA GTGGGGACAC AGCCATTGGG 
ACATCATGCT CAGACATTGC AGAGGATTTT TGCAGCTCAA GTGGCAGCCC TTCCTTCCAG 
CCAATCAAAA GCCACATAAC CATTCCAACA GCCCATGTGA TGCCTTCTAC TTTGGGGGCC 
TCTCCTGCCA AACCAAATTC TGCACCTTCC GGGCCTTCTT CCGCTAAGCT GCCCTTGTCA 
GGATTGACTG AAGGCGTGGG GATGACAAGA AATGGAGACT TCGGAGCTGT GAAGCGCTCT 
CCAGGCCTAG CAAGGGATTT TATGTACCTC CCTAGTGCTG CCGGGGAGAA TGGGAGTCAG 
CAGTCCTGGT TCCCAGCAGT GGGCCACGAG AGAGAGGGGG AGATGAGGAA GTTCGATGTT 
CCTAGCATGG AGTCTACCCT TAACCAGCCA GCCATGCTAG AGACGTTATA CTCAGATCCA 
CATTACCGAG CCCATTTCCC CAACCCAAGA CCTGATACAA ATAAGGATGT ATACAAAGTA 
TTGCCAGAAT CCAAGAAGGC ACCGGGCAGT GGTGCAGTAT TTGAGAGGAA CGGACCACAT 
GCTAGCAGTA GTGGGGTGCT CCCTTTGGGA CTCCAGCCTG CGCCTGGACT TTCCAAGTCA 
CTATCCTCTC AGGTGTGGCA ACCAAGTCCT GACCCTTGGC ATCCTGGAGA ACAATCCTGT 
GAACTCAGTA CTTGTCGACA GCAGTTGGAA TTGATCCGTT TACAGATGGA GCAAATGCAG 
CTTCAGAACG GAGCCATGTG TCACCATCCT GCTGCTTTCG CTCCATTACT GCCCACCCTA 
GAGCCAGCAC AGTGGCTCAG CATCCTGAAC AGTAACGAGC ATCTCCTGAA GGAGAAGGAG 
CTCCTCATTG ACAAGCAAAG GAAGCATATC TCTCAGCTGG AGCAGAAAGT GCGAGAGAGT 
GAACTGCAAG TCCACAGTGC CCTTTTGGGC CGCCCTGCCC CCTTTGGGGA TGTCTGCTTA 
TTGAGGCTAC AGGAGTTGCA GCGAGAGAAC ACTTTCTTAC GGGCACAGTT TGCACAGAAG 
ACAGAAGCCC TGAGCAAGGA GAAGATGGAG CTTGAAAAGA AACTCTCTGC ATCTGAAGTT 
GAAATTCAGC TCATTAGGGA GTCTCTAAAA GTGACACTAC AGAAGCATTC GGAGGAGGGG 
AAGAAACAGG AGGAAAGGGT CAAAGGTCGT GATAAACATA TCAATAATTT GAAAAAGAAA 
TGTCAGAAGG AATCAGAGCA GAACCGGGAG AAGCAGCAGC GTATTGAAAC CTTGGAGCGC 
TATCTAGCTG ACCTGCCCAC CCTAGAAGAC CATCAGAAAC AGACGGAGCA GCTTAAGGAC 
GCTGAATTAA AGAACACAGA ACTGCAAGAG AGAGTGGCTG AGCTGGAGAC TTTGCTGGAG 
GACACCCAGG CAACCTGCAG AGAGAAGGAG GTTCAGCTGG AAAGTCTGAG ACAAAGAGAA 
GCAGACCTCT CCTCTGCTAG ACATAGCTTT CAAGATAAAC AGTCTGTGGA GGAAGCCAAT 
GGAGAAAACC TCAGAGTGGA CATGGAATCC CAGCAGAAGG AATGTGACTC CCTCCGAAAG 
ATGGTGGAGA GGCAACAGCT GAAGATGGAA CAGCTGCACT CCCAAGTCCA GAGCCAAAAG 
CAAGAACTGG CACAAGAAGA GGGAATCAAC CAGGCACTGA GAGAGGAGGC CCAGCGGAGG 
GAGACGGCCC TGCAGCAGAT GCGCACAGCC GTGAAGGAGC TTTCAGTGCA AAACCAGGAC 
CTGATTGAGA AGAATCTGAC GCTGCAAGAG CACTTGCGCC AGGCCCAGCC AGGGTCCTCA 
TCTTCACCAG ACTCAGCCCA GCTGGCCTGT GAGCTGCACC AGGAACTGGC CAGTTGCCTT 
CAAGATCTGC AAGCTGTCTG CAGCATTGTG ACCCAGAGGG CCCAGGGCCA CAATCCTAAC 
CTCTCACTAC TCCTAGGCAT TCACTCCACC CAGCACCCAG GGACGCAGCT AGATCTACAA 
AAGCCAGATG TGATCAGGAG GAAACTAGAA GAGGTTCAAC AGCTGCGCCA TGACATTGAG 
GACTTAAGGA CCAGCCTGTC AGACAGATAT GCCCAGGACA TGGGAGAAAA CTGTGCCACA 
CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu21139
Clone ID Related Accession (Same CDS sequence) XM_011250330.2 , NM_144527.3
Accession Version XM_011250330.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product centrosomal protein of 85 kDa isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250330.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCCATGC AAGAAAAATA CCCAAATGAC AGGTCTCATG CTACTTCACC AGGTTCCAAT 
GTGATTCAAA AAGGCAGTTC TCTGGGAACT GAGTGGCAGA CACCAGTTAT CTCAGAGACC 
TTTCGGAGCC GCTTCAGCCG CTGCTCAAGC ATTGCTGACA GTGGGGACAC AGCCATTGGG 
ACATCATGCT CAGACATTGC AGAGGATTTT TGCAGCTCAA GTGGCAGCCC TTCCTTCCAG 
CCAATCAAAA GCCACATAAC CATTCCAACA GCCCATGTGA TGCCTTCTAC TTTGGGGGCC 
TCTCCTGCCA AACCAAATTC TGCACCTTCC GGGCCTTCTT CCGCTAAGCT GCCCTTGTCA 
GGATTGACTG AAGGCGTGGG GATGACAAGA AATGGAGACT TCGGAGCTGT GAAGCGCTCT 
CCAGGCCTAG CAAGGGATTT TATGTACCTC CCTAGTGCTG CCGGGGAGAA TGGGAGTCAG 
CAGTCCTGGT TCCCAGCAGT GGGCCACGAG AGAGAGGGGG AGATGAGGAA GTTCGATGTT 
CCTAGCATGG AGTCTACCCT TAACCAGCCA GCCATGCTAG AGACGTTATA CTCAGATCCA 
CATTACCGAG CCCATTTCCC CAACCCAAGA CCTGATACAA ATAAGGATGT ATACAAAGTA 
TTGCCAGAAT CCAAGAAGGC ACCGGGCAGT GGTGCAGTAT TTGAGAGGAA CGGACCACAT 
GCTAGCAGTA GTGGGGTGCT CCCTTTGGGA CTCCAGCCTG CGCCTGGACT TTCCAAGTCA 
CTATCCTCTC AGGTGTGGCA ACCAAGTCCT GACCCTTGGC ATCCTGGAGA ACAATCCTGT 
GAACTCAGTA CTTGTCGACA GCAGTTGGAA TTGATCCGTT TACAGATGGA GCAAATGCAG 
CTTCAGAACG GAGCCATGTG TCACCATCCT GCTGCTTTCG CTCCATTACT GCCCACCCTA 
GAGCCAGCAC AGTGGCTCAG CATCCTGAAC AGTAACGAGC ATCTCCTGAA GGAGAAGGAG 
CTCCTCATTG ACAAGCAAAG GAAGCATATC TCTCAGCTGG AGCAGAAAGT GCGAGAGAGT 
GAACTGCAAG TCCACAGTGC CCTTTTGGGC CGCCCTGCCC CCTTTGGGGA TGTCTGCTTA 
TTGAGGCTAC AGGAGTTGCA GCGAGAGAAC ACTTTCTTAC GGGCACAGTT TGCACAGAAG 
ACAGAAGCCC TGAGCAAGGA GAAGATGGAG CTTGAAAAGA AACTCTCTGC ATCTGAAGTT 
GAAATTCAGC TCATTAGGGA GTCTCTAAAA GTGACACTAC AGAAGCATTC GGAGGAGGGG 
AAGAAACAGG AGGAAAGGGT CAAAGGTCGT GATAAACATA TCAATAATTT GAAAAAGAAA 
TGTCAGAAGG AATCAGAGCA GAACCGGGAG AAGCAGCAGC GTATTGAAAC CTTGGAGCGC 
TATCTAGCTG ACCTGCCCAC CCTAGAAGAC CATCAGAAAC AGACGGAGCA GCTTAAGGAC 
GCTGAATTAA AGAACACAGA ACTGCAAGAG AGAGTGGCTG AGCTGGAGAC TTTGCTGGAG 
GACACCCAGG CAACCTGCAG AGAGAAGGAG GTTCAGCTGG AAAGTCTGAG ACAAAGAGAA 
GCAGACCTCT CCTCTGCTAG ACATAGCTTT CAAGATAAAC AGTCTGTGGA GGAAGCCAAT 
GGAGAAAACC TCAGAGTGGA CATGGAATCC CAGCAGAAGG AATGTGACTC CCTCCGAAAG 
ATGGTGGAGA GGCAACAGCT GAAGATGGAA CAGCTGCACT CCCAAGTCCA GAGCCAAAAG 
CAAGAACTGG CACAAGAAGA GGGAATCAAC CAGGCACTGA GAGAGGAGGC CCAGCGGAGG 
GAGACGGCCC TGCAGCAGAT GCGCACAGCC GTGAAGGAGC TTTCAGTGCA AAACCAGGAC 
CTGATTGAGA AGAATCTGAC GCTGCAAGAG CACTTGCGCC AGGCCCAGCC AGGGTCCTCA 
TCTTCACCAG ACTCAGCCCA GCTGGCCTGT GAGCTGCACC AGGAACTGGC CAGTTGCCTT 
CAAGATCTGC AAGCTGTCTG CAGCATTGTG ACCCAGAGGG CCCAGGGCCA CAATCCTAAC 
CTCTCACTAC TCCTAGGCAT TCACTCCACC CAGCACCCAG GGACGCAGCT AGATCTACAA 
AAGCCAGATG TGATCAGGAG GAAACTAGAA GAGGTTCAAC AGCTGCGCCA TGACATTGAG 
GACTTAAGGA CCAGCCTGTC AGACAGATAT GCCCAGGACA TGGGAGAAAA CTGTGCCACA 
CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011248632.1
CDS323..2608
Translation

Target ORF information:

RefSeq Version XM_011250330.2
Organism Mus musculus(house mouse)
Definition Mus musculus centrosomal protein 85 (Cep85), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011250330.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCCATGC AAGAAAAATA CCCAAATGAC AGGTCTCATG CTACTTCACC AGGTTCCAAT 
GTGATTCAAA AAGGCAGTTC TCTGGGAACT GAGTGGCAGA CACCAGTTAT CTCAGAGACC 
TTTCGGAGCC GCTTCAGCCG CTGCTCAAGC ATTGCTGACA GTGGGGACAC AGCCATTGGG 
ACATCATGCT CAGACATTGC AGAGGATTTT TGCAGCTCAA GTGGCAGCCC TTCCTTCCAG 
CCAATCAAAA GCCACATAAC CATTCCAACA GCCCATGTGA TGCCTTCTAC TTTGGGGGCC 
TCTCCTGCCA AACCAAATTC TGCACCTTCC GGGCCTTCTT CCGCTAAGCT GCCCTTGTCA 
GGATTGACTG AAGGCGTGGG GATGACAAGA AATGGAGACT TCGGAGCTGT GAAGCGCTCT 
CCAGGCCTAG CAAGGGATTT TATGTACCTC CCTAGTGCTG CCGGGGAGAA TGGGAGTCAG 
CAGTCCTGGT TCCCAGCAGT GGGCCACGAG AGAGAGGGGG AGATGAGGAA GTTCGATGTT 
CCTAGCATGG AGTCTACCCT TAACCAGCCA GCCATGCTAG AGACGTTATA CTCAGATCCA 
CATTACCGAG CCCATTTCCC CAACCCAAGA CCTGATACAA ATAAGGATGT ATACAAAGTA 
TTGCCAGAAT CCAAGAAGGC ACCGGGCAGT GGTGCAGTAT TTGAGAGGAA CGGACCACAT 
GCTAGCAGTA GTGGGGTGCT CCCTTTGGGA CTCCAGCCTG CGCCTGGACT TTCCAAGTCA 
CTATCCTCTC AGGTGTGGCA ACCAAGTCCT GACCCTTGGC ATCCTGGAGA ACAATCCTGT 
GAACTCAGTA CTTGTCGACA GCAGTTGGAA TTGATCCGTT TACAGATGGA GCAAATGCAG 
CTTCAGAACG GAGCCATGTG TCACCATCCT GCTGCTTTCG CTCCATTACT GCCCACCCTA 
GAGCCAGCAC AGTGGCTCAG CATCCTGAAC AGTAACGAGC ATCTCCTGAA GGAGAAGGAG 
CTCCTCATTG ACAAGCAAAG GAAGCATATC TCTCAGCTGG AGCAGAAAGT GCGAGAGAGT 
GAACTGCAAG TCCACAGTGC CCTTTTGGGC CGCCCTGCCC CCTTTGGGGA TGTCTGCTTA 
TTGAGGCTAC AGGAGTTGCA GCGAGAGAAC ACTTTCTTAC GGGCACAGTT TGCACAGAAG 
ACAGAAGCCC TGAGCAAGGA GAAGATGGAG CTTGAAAAGA AACTCTCTGC ATCTGAAGTT 
GAAATTCAGC TCATTAGGGA GTCTCTAAAA GTGACACTAC AGAAGCATTC GGAGGAGGGG 
AAGAAACAGG AGGAAAGGGT CAAAGGTCGT GATAAACATA TCAATAATTT GAAAAAGAAA 
TGTCAGAAGG AATCAGAGCA GAACCGGGAG AAGCAGCAGC GTATTGAAAC CTTGGAGCGC 
TATCTAGCTG ACCTGCCCAC CCTAGAAGAC CATCAGAAAC AGACGGAGCA GCTTAAGGAC 
GCTGAATTAA AGAACACAGA ACTGCAAGAG AGAGTGGCTG AGCTGGAGAC TTTGCTGGAG 
GACACCCAGG CAACCTGCAG AGAGAAGGAG GTTCAGCTGG AAAGTCTGAG ACAAAGAGAA 
GCAGACCTCT CCTCTGCTAG ACATAGCTTT CAAGATAAAC AGTCTGTGGA GGAAGCCAAT 
GGAGAAAACC TCAGAGTGGA CATGGAATCC CAGCAGAAGG AATGTGACTC CCTCCGAAAG 
ATGGTGGAGA GGCAACAGCT GAAGATGGAA CAGCTGCACT CCCAAGTCCA GAGCCAAAAG 
CAAGAACTGG CACAAGAAGA GGGAATCAAC CAGGCACTGA GAGAGGAGGC CCAGCGGAGG 
GAGACGGCCC TGCAGCAGAT GCGCACAGCC GTGAAGGAGC TTTCAGTGCA AAACCAGGAC 
CTGATTGAGA AGAATCTGAC GCTGCAAGAG CACTTGCGCC AGGCCCAGCC AGGGTCCTCA 
TCTTCACCAG ACTCAGCCCA GCTGGCCTGT GAGCTGCACC AGGAACTGGC CAGTTGCCTT 
CAAGATCTGC AAGCTGTCTG CAGCATTGTG ACCCAGAGGG CCCAGGGCCA CAATCCTAAC 
CTCTCACTAC TCCTAGGCAT TCACTCCACC CAGCACCCAG GGACGCAGCT AGATCTACAA 
AAGCCAGATG TGATCAGGAG GAAACTAGAA GAGGTTCAAC AGCTGCGCCA TGACATTGAG 
GACTTAAGGA CCAGCCTGTC AGACAGATAT GCCCAGGACA TGGGAGAAAA CTGTGCCACA 
CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu34423
Clone ID Related Accession (Same CDS sequence) XM_006539164.1
Accession Version XM_006539164.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2097bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product centrosomal protein of 85 kDa isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAAAGACC CCCGAGATTT TTGCAGCTCA AGTGGCAGCC CTTCCTTCCA GCCAATCAAA 
AGCCACATAA CCATTCCAAC AGCCCATGTG ATGCCTTCTA CTTTGGGGGC CTCTCCTGCC 
AAACCAAATT CTGCACCTTC CGGGCCTTCT TCCGCTAAGC TGCCCTTGTC AGGATTGACT 
GAAGGCGTGG GGATGACAAG AAATGGAGAC TTCGGAGCTG TGAAGCGCTC TCCAGGCCTA 
GCAAGGGATT TTATGTACCT CCCTAGTGCT GCCGGGGAGA ATGGGAGTCA GCAGTCCTGG 
TTCCCAGCAG TGGGCCACGA GAGAGAGGGG GAGATGAGGA AGTTCGATGT TCCTAGCATG 
GAGTCTACCC TTAACCAGCC AGCCATGCTA GAGACGTTAT ACTCAGATCC ACATTACCGA 
GCCCATTTCC CCAACCCAAG ACCTGATACA AATAAGGATG TATACAAAGT ATTGCCAGAA 
TCCAAGAAGG CACCGGGCAG TGGTGCAGTA TTTGAGAGGA ACGGACCACA TGCTAGCAGT 
AGTGGGGTGC TCCCTTTGGG ACTCCAGCCT GCGCCTGGAC TTTCCAAGTC ACTATCCTCT 
CAGGTGTGGC AACCAAGTCC TGACCCTTGG CATCCTGGAG AACAATCCTG TGAACTCAGT 
ACTTGTCGAC AGCAGTTGGA ATTGATCCGT TTACAGATGG AGCAAATGCA GCTTCAGAAC 
GGAGCCATGT GTCACCATCC TGCTGCTTTC GCTCCATTAC TGCCCACCCT AGAGCCAGCA 
CAGTGGCTCA GCATCCTGAA CAGTAACGAG CATCTCCTGA AGGAGAAGGA GCTCCTCATT 
GACAAGCAAA GGAAGCATAT CTCTCAGCTG GAGCAGAAAG TGCGAGAGAG TGAACTGCAA 
GTCCACAGTG CCCTTTTGGG CCGCCCTGCC CCCTTTGGGG ATGTCTGCTT ATTGAGGCTA 
CAGGAGTTGC AGCGAGAGAA CACTTTCTTA CGGGCACAGT TTGCACAGAA GACAGAAGCC 
CTGAGCAAGG AGAAGATGGA GCTTGAAAAG AAACTCTCTG CATCTGAAGT TGAAATTCAG 
CTCATTAGGG AGTCTCTAAA AGTGACACTA CAGAAGCATT CGGAGGAGGG GAAGAAACAG 
GAGGAAAGGG TCAAAGGTCG TGATAAACAT ATCAATAATT TGAAAAAGAA ATGTCAGAAG 
GAATCAGAGC AGAACCGGGA GAAGCAGCAG CGTATTGAAA CCTTGGAGCG CTATCTAGCT 
GACCTGCCCA CCCTAGAAGA CCATCAGAAA CAGACGGAGC AGCTTAAGGA CGCTGAATTA 
AAGAACACAG AACTGCAAGA GAGAGTGGCT GAGCTGGAGA CTTTGCTGGA GGACACCCAG 
GCAACCTGCA GAGAGAAGGA GGTTCAGCTG GAAAGTCTGA GACAAAGAGA AGCAGACCTC 
TCCTCTGCTA GACATAGCTT TCAAGATAAA CAGTCTGTGG AGGAAGCCAA TGGAGAAAAC 
CTCAGAGTGG ACATGGAATC CCAGCAGAAG GAATGTGACT CCCTCCGAAA GATGGTGGAG 
AGGCAACAGC TGAAGATGGA ACAGCTGCAC TCCCAAGTCC AGAGCCAAAA GCAAGAACTG 
GCACAAGAAG AGGGAATCAA CCAGGCACTG AGAGAGGAGG CCCAGCGGAG GGAGACGGCC 
CTGCAGCAGA TGCGCACAGC CGTGAAGGAG CTTTCAGTGC AAAACCAGGA CCTGATTGAG 
AAGAATCTGA CGCTGCAAGA GCACTTGCGC CAGGCCCAGC CAGGGTCCTC ATCTTCACCA 
GACTCAGCCC AGCTGGCCTG TGAGCTGCAC CAGGAACTGG CCAGTTGCCT TCAAGATCTG 
CAAGCTGTCT GCAGCATTGT GACCCAGAGG GCCCAGGGCC ACAATCCTAA CCTCTCACTA 
CTCCTAGGCA TTCACTCCAC CCAGCACCCA GGGACGCAGC TAGATCTACA AAAGCCAGAT 
GTGATCAGGA GGAAACTAGA AGAGGTTCAA CAGCTGCGCC ATGACATTGA GGACTTAAGG 
ACCAGCCTGT CAGACAGATA TGCCCAGGAC ATGGGAGAAA ACTGTGCCAC ACAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539227.1
CDS26..2122
Translation

Target ORF information:

RefSeq Version XM_006539164.1
Organism Mus musculus(house mouse)
Definition Mus musculus centrosomal protein 85 (Cep85), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539164.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAAAGACC CCCGAGATTT TTGCAGCTCA AGTGGCAGCC CTTCCTTCCA GCCAATCAAA 
AGCCACATAA CCATTCCAAC AGCCCATGTG ATGCCTTCTA CTTTGGGGGC CTCTCCTGCC 
AAACCAAATT CTGCACCTTC CGGGCCTTCT TCCGCTAAGC TGCCCTTGTC AGGATTGACT 
GAAGGCGTGG GGATGACAAG AAATGGAGAC TTCGGAGCTG TGAAGCGCTC TCCAGGCCTA 
GCAAGGGATT TTATGTACCT CCCTAGTGCT GCCGGGGAGA ATGGGAGTCA GCAGTCCTGG 
TTCCCAGCAG TGGGCCACGA GAGAGAGGGG GAGATGAGGA AGTTCGATGT TCCTAGCATG 
GAGTCTACCC TTAACCAGCC AGCCATGCTA GAGACGTTAT ACTCAGATCC ACATTACCGA 
GCCCATTTCC CCAACCCAAG ACCTGATACA AATAAGGATG TATACAAAGT ATTGCCAGAA 
TCCAAGAAGG CACCGGGCAG TGGTGCAGTA TTTGAGAGGA ACGGACCACA TGCTAGCAGT 
AGTGGGGTGC TCCCTTTGGG ACTCCAGCCT GCGCCTGGAC TTTCCAAGTC ACTATCCTCT 
CAGGTGTGGC AACCAAGTCC TGACCCTTGG CATCCTGGAG AACAATCCTG TGAACTCAGT 
ACTTGTCGAC AGCAGTTGGA ATTGATCCGT TTACAGATGG AGCAAATGCA GCTTCAGAAC 
GGAGCCATGT GTCACCATCC TGCTGCTTTC GCTCCATTAC TGCCCACCCT AGAGCCAGCA 
CAGTGGCTCA GCATCCTGAA CAGTAACGAG CATCTCCTGA AGGAGAAGGA GCTCCTCATT 
GACAAGCAAA GGAAGCATAT CTCTCAGCTG GAGCAGAAAG TGCGAGAGAG TGAACTGCAA 
GTCCACAGTG CCCTTTTGGG CCGCCCTGCC CCCTTTGGGG ATGTCTGCTT ATTGAGGCTA 
CAGGAGTTGC AGCGAGAGAA CACTTTCTTA CGGGCACAGT TTGCACAGAA GACAGAAGCC 
CTGAGCAAGG AGAAGATGGA GCTTGAAAAG AAACTCTCTG CATCTGAAGT TGAAATTCAG 
CTCATTAGGG AGTCTCTAAA AGTGACACTA CAGAAGCATT CGGAGGAGGG GAAGAAACAG 
GAGGAAAGGG TCAAAGGTCG TGATAAACAT ATCAATAATT TGAAAAAGAA ATGTCAGAAG 
GAATCAGAGC AGAACCGGGA GAAGCAGCAG CGTATTGAAA CCTTGGAGCG CTATCTAGCT 
GACCTGCCCA CCCTAGAAGA CCATCAGAAA CAGACGGAGC AGCTTAAGGA CGCTGAATTA 
AAGAACACAG AACTGCAAGA GAGAGTGGCT GAGCTGGAGA CTTTGCTGGA GGACACCCAG 
GCAACCTGCA GAGAGAAGGA GGTTCAGCTG GAAAGTCTGA GACAAAGAGA AGCAGACCTC 
TCCTCTGCTA GACATAGCTT TCAAGATAAA CAGTCTGTGG AGGAAGCCAA TGGAGAAAAC 
CTCAGAGTGG ACATGGAATC CCAGCAGAAG GAATGTGACT CCCTCCGAAA GATGGTGGAG 
AGGCAACAGC TGAAGATGGA ACAGCTGCAC TCCCAAGTCC AGAGCCAAAA GCAAGAACTG 
GCACAAGAAG AGGGAATCAA CCAGGCACTG AGAGAGGAGG CCCAGCGGAG GGAGACGGCC 
CTGCAGCAGA TGCGCACAGC CGTGAAGGAG CTTTCAGTGC AAAACCAGGA CCTGATTGAG 
AAGAATCTGA CGCTGCAAGA GCACTTGCGC CAGGCCCAGC CAGGGTCCTC ATCTTCACCA 
GACTCAGCCC AGCTGGCCTG TGAGCTGCAC CAGGAACTGG CCAGTTGCCT TCAAGATCTG 
CAAGCTGTCT GCAGCATTGT GACCCAGAGG GCCCAGGGCC ACAATCCTAA CCTCTCACTA 
CTCCTAGGCA TTCACTCCAC CCAGCACCCA GGGACGCAGC TAGATCTACA AAAGCCAGAT 
GTGATCAGGA GGAAACTAGA AGAGGTTCAA CAGCTGCGCC ATGACATTGA GGACTTAAGG 
ACCAGCCTGT CAGACAGATA TGCCCAGGAC ATGGGAGAAA ACTGTGCCAC ACAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu70486
Clone ID Related Accession (Same CDS sequence) XM_011250331.2
Accession Version XM_011250331.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2133bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product centrosomal protein of 85 kDa isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGCCATGC AAGAAAAATA CCCAAATGAC AGGTCTCATG CTACTTCACC AGATTTTTGC 
AGCTCAAGTG GCAGCCCTTC CTTCCAGCCA ATCAAAAGCC ACATAACCAT TCCAACAGCC 
CATGTGATGC CTTCTACTTT GGGGGCCTCT CCTGCCAAAC CAAATTCTGC ACCTTCCGGG 
CCTTCTTCCG CTAAGCTGCC CTTGTCAGGA TTGACTGAAG GCGTGGGGAT GACAAGAAAT 
GGAGACTTCG GAGCTGTGAA GCGCTCTCCA GGCCTAGCAA GGGATTTTAT GTACCTCCCT 
AGTGCTGCCG GGGAGAATGG GAGTCAGCAG TCCTGGTTCC CAGCAGTGGG CCACGAGAGA 
GAGGGGGAGA TGAGGAAGTT CGATGTTCCT AGCATGGAGT CTACCCTTAA CCAGCCAGCC 
ATGCTAGAGA CGTTATACTC AGATCCACAT TACCGAGCCC ATTTCCCCAA CCCAAGACCT 
GATACAAATA AGGATGTATA CAAAGTATTG CCAGAATCCA AGAAGGCACC GGGCAGTGGT 
GCAGTATTTG AGAGGAACGG ACCACATGCT AGCAGTAGTG GGGTGCTCCC TTTGGGACTC 
CAGCCTGCGC CTGGACTTTC CAAGTCACTA TCCTCTCAGG TGTGGCAACC AAGTCCTGAC 
CCTTGGCATC CTGGAGAACA ATCCTGTGAA CTCAGTACTT GTCGACAGCA GTTGGAATTG 
ATCCGTTTAC AGATGGAGCA AATGCAGCTT CAGAACGGAG CCATGTGTCA CCATCCTGCT 
GCTTTCGCTC CATTACTGCC CACCCTAGAG CCAGCACAGT GGCTCAGCAT CCTGAACAGT 
AACGAGCATC TCCTGAAGGA GAAGGAGCTC CTCATTGACA AGCAAAGGAA GCATATCTCT 
CAGCTGGAGC AGAAAGTGCG AGAGAGTGAA CTGCAAGTCC ACAGTGCCCT TTTGGGCCGC 
CCTGCCCCCT TTGGGGATGT CTGCTTATTG AGGCTACAGG AGTTGCAGCG AGAGAACACT 
TTCTTACGGG CACAGTTTGC ACAGAAGACA GAAGCCCTGA GCAAGGAGAA GATGGAGCTT 
GAAAAGAAAC TCTCTGCATC TGAAGTTGAA ATTCAGCTCA TTAGGGAGTC TCTAAAAGTG 
ACACTACAGA AGCATTCGGA GGAGGGGAAG AAACAGGAGG AAAGGGTCAA AGGTCGTGAT 
AAACATATCA ATAATTTGAA AAAGAAATGT CAGAAGGAAT CAGAGCAGAA CCGGGAGAAG 
CAGCAGCGTA TTGAAACCTT GGAGCGCTAT CTAGCTGACC TGCCCACCCT AGAAGACCAT 
CAGAAACAGA CGGAGCAGCT TAAGGACGCT GAATTAAAGA ACACAGAACT GCAAGAGAGA 
GTGGCTGAGC TGGAGACTTT GCTGGAGGAC ACCCAGGCAA CCTGCAGAGA GAAGGAGGTT 
CAGCTGGAAA GTCTGAGACA AAGAGAAGCA GACCTCTCCT CTGCTAGACA TAGCTTTCAA 
GATAAACAGT CTGTGGAGGA AGCCAATGGA GAAAACCTCA GAGTGGACAT GGAATCCCAG 
CAGAAGGAAT GTGACTCCCT CCGAAAGATG GTGGAGAGGC AACAGCTGAA GATGGAACAG 
CTGCACTCCC AAGTCCAGAG CCAAAAGCAA GAACTGGCAC AAGAAGAGGG AATCAACCAG 
GCACTGAGAG AGGAGGCCCA GCGGAGGGAG ACGGCCCTGC AGCAGATGCG CACAGCCGTG 
AAGGAGCTTT CAGTGCAAAA CCAGGACCTG ATTGAGAAGA ATCTGACGCT GCAAGAGCAC 
TTGCGCCAGG CCCAGCCAGG GTCCTCATCT TCACCAGACT CAGCCCAGCT GGCCTGTGAG 
CTGCACCAGG AACTGGCCAG TTGCCTTCAA GATCTGCAAG CTGTCTGCAG CATTGTGACC 
CAGAGGGCCC AGGGCCACAA TCCTAACCTC TCACTACTCC TAGGCATTCA CTCCACCCAG 
CACCCAGGGA CGCAGCTAGA TCTACAAAAG CCAGATGTGA TCAGGAGGAA ACTAGAAGAG 
GTTCAACAGC TGCGCCATGA CATTGAGGAC TTAAGGACCA GCCTGTCAGA CAGATATGCC 
CAGGACATGG GAGAAAACTG TGCCACACAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011248633.1
CDS149..2281
Translation

Target ORF information:

RefSeq Version XM_011250331.2
Organism Mus musculus(house mouse)
Definition Mus musculus centrosomal protein 85 (Cep85), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011250331.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGCCATGC AAGAAAAATA CCCAAATGAC AGGTCTCATG CTACTTCACC AGATTTTTGC 
AGCTCAAGTG GCAGCCCTTC CTTCCAGCCA ATCAAAAGCC ACATAACCAT TCCAACAGCC 
CATGTGATGC CTTCTACTTT GGGGGCCTCT CCTGCCAAAC CAAATTCTGC ACCTTCCGGG 
CCTTCTTCCG CTAAGCTGCC CTTGTCAGGA TTGACTGAAG GCGTGGGGAT GACAAGAAAT 
GGAGACTTCG GAGCTGTGAA GCGCTCTCCA GGCCTAGCAA GGGATTTTAT GTACCTCCCT 
AGTGCTGCCG GGGAGAATGG GAGTCAGCAG TCCTGGTTCC CAGCAGTGGG CCACGAGAGA 
GAGGGGGAGA TGAGGAAGTT CGATGTTCCT AGCATGGAGT CTACCCTTAA CCAGCCAGCC 
ATGCTAGAGA CGTTATACTC AGATCCACAT TACCGAGCCC ATTTCCCCAA CCCAAGACCT 
GATACAAATA AGGATGTATA CAAAGTATTG CCAGAATCCA AGAAGGCACC GGGCAGTGGT 
GCAGTATTTG AGAGGAACGG ACCACATGCT AGCAGTAGTG GGGTGCTCCC TTTGGGACTC 
CAGCCTGCGC CTGGACTTTC CAAGTCACTA TCCTCTCAGG TGTGGCAACC AAGTCCTGAC 
CCTTGGCATC CTGGAGAACA ATCCTGTGAA CTCAGTACTT GTCGACAGCA GTTGGAATTG 
ATCCGTTTAC AGATGGAGCA AATGCAGCTT CAGAACGGAG CCATGTGTCA CCATCCTGCT 
GCTTTCGCTC CATTACTGCC CACCCTAGAG CCAGCACAGT GGCTCAGCAT CCTGAACAGT 
AACGAGCATC TCCTGAAGGA GAAGGAGCTC CTCATTGACA AGCAAAGGAA GCATATCTCT 
CAGCTGGAGC AGAAAGTGCG AGAGAGTGAA CTGCAAGTCC ACAGTGCCCT TTTGGGCCGC 
CCTGCCCCCT TTGGGGATGT CTGCTTATTG AGGCTACAGG AGTTGCAGCG AGAGAACACT 
TTCTTACGGG CACAGTTTGC ACAGAAGACA GAAGCCCTGA GCAAGGAGAA GATGGAGCTT 
GAAAAGAAAC TCTCTGCATC TGAAGTTGAA ATTCAGCTCA TTAGGGAGTC TCTAAAAGTG 
ACACTACAGA AGCATTCGGA GGAGGGGAAG AAACAGGAGG AAAGGGTCAA AGGTCGTGAT 
AAACATATCA ATAATTTGAA AAAGAAATGT CAGAAGGAAT CAGAGCAGAA CCGGGAGAAG 
CAGCAGCGTA TTGAAACCTT GGAGCGCTAT CTAGCTGACC TGCCCACCCT AGAAGACCAT 
CAGAAACAGA CGGAGCAGCT TAAGGACGCT GAATTAAAGA ACACAGAACT GCAAGAGAGA 
GTGGCTGAGC TGGAGACTTT GCTGGAGGAC ACCCAGGCAA CCTGCAGAGA GAAGGAGGTT 
CAGCTGGAAA GTCTGAGACA AAGAGAAGCA GACCTCTCCT CTGCTAGACA TAGCTTTCAA 
GATAAACAGT CTGTGGAGGA AGCCAATGGA GAAAACCTCA GAGTGGACAT GGAATCCCAG 
CAGAAGGAAT GTGACTCCCT CCGAAAGATG GTGGAGAGGC AACAGCTGAA GATGGAACAG 
CTGCACTCCC AAGTCCAGAG CCAAAAGCAA GAACTGGCAC AAGAAGAGGG AATCAACCAG 
GCACTGAGAG AGGAGGCCCA GCGGAGGGAG ACGGCCCTGC AGCAGATGCG CACAGCCGTG 
AAGGAGCTTT CAGTGCAAAA CCAGGACCTG ATTGAGAAGA ATCTGACGCT GCAAGAGCAC 
TTGCGCCAGG CCCAGCCAGG GTCCTCATCT TCACCAGACT CAGCCCAGCT GGCCTGTGAG 
CTGCACCAGG AACTGGCCAG TTGCCTTCAA GATCTGCAAG CTGTCTGCAG CATTGTGACC 
CAGAGGGCCC AGGGCCACAA TCCTAACCTC TCACTACTCC TAGGCATTCA CTCCACCCAG 
CACCCAGGGA CGCAGCTAGA TCTACAAAAG CCAGATGTGA TCAGGAGGAA ACTAGAAGAG 
GTTCAACAGC TGCGCCATGA CATTGAGGAC TTAAGGACCA GCCTGTCAGA CAGATATGCC 
CAGGACATGG GAGAAAACTG TGCCACACAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Characterization of Cep85 - a new antagonist of Nek2A that is involved in the regulation of centrosome disjunction.
Journal of cell science128(17)3290-303(2015 Sep)
Chen C,Tian F,Lu L,Wang Y,Xiao Z,Yu C,Yu X


book

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K


book

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE


book

Exchangeable gene trap using the Cre/mutated lox system.
Cellular and molecular biology (Noisy-le-Grand, France)45(5)737-50(1999 Jul)
Araki K,Imaizumi T,Sekimoto T,Yoshinobu K,Yoshimuta J,Akizuki M,Miura K,Araki M,Yamamura K