Hspb8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hspb8
Entrez Gene ID	80888
Full Name	heat shock protein 8
Synonyms	AU018630,AW413033,Cryac,D5Ucla4,E2IG1,H11,H11K,HSP20-like,HSP22
General protein information	Preferred Names heat shock protein 8 Names heat shock protein beta-8 alpha-crystallin C chain crystallin, alpha C heat shock 27kDa protein 8 heat shock protein 20-like protein small stress protein-like protein HSP22
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 F\|5 56.41 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_030704.3	NP_109629.1	heat shock protein beta-8

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	hspb8	NP_001094427.2
Homo sapiens (human)	HSPB8	NP_055180.1
Pan troglodytes (chimpanzee)	HSPB8	XP_509417.1
Canis lupus familiaris (dog)	HSPB8	NP_001003029.1
Xenopus tropicalis (tropical clawed frog)	hspb8	NP_001005658.1
Mus musculus (house mouse)	Hspb8	NP_109629.1
Gallus gallus (chicken)	HSPB8	XP_415280.1
Bos taurus (cattle)	HSPB8	NP_001014955.1
	HSPB8	NP_001028109.1
Rattus norvegicus (Norway rat)	Hspb8	NP_446064.1

Gene Ontology
Bibliography

Related articles in PubMed

A knock-in/knock-out mouse model of HSPB8-associated distal hereditary motor neuropathy and myopathy reveals toxic gain-of-function of mutant Hspb8.
Bouhy D, Juneja M, Katona I, Holmgren A, Asselbergh B, De Winter V, Hochepied T, Goossens S, Haigh JJ, Libert C, Ceuterick-de Groote C, Irobi J, Weis J, Timmerman V
Acta neuropathologica135(1)131-148(2018 Jan)

Heat shock protein 22 (HSPB8) limits TGF-?-stimulated migration of osteoblasts.
Yamamoto N, Tokuda H, Kuroyanagi G, Kainuma S, Matsushima-Nishiwaki R, Fujita K, Kozawa O, Otsuka T
Molecular and cellular endocrinology4361-9(2016 Nov)

HspB8 mediates neuroprotection against OGD/R in N2A cells through the phosphoinositide 3-kinase/Akt pathway.
Hu Z, Yang B, Mo X, Zhou F
Brain research164415-21(2016 08)

Hsp20 Protects against Oxygen-Glucose Deprivation/Reperfusion-Induced Golgi Fragmentation and Apoptosis through Fas/FasL Pathway.
Zhong B, Hu Z, Tan J, Lu T, Lei Q, Chen C, Zeng L
Oxidative medicine and cellular longevity2015606934(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HSP22 functions as a negative regulator in the TGF-beta-stimulated migration of osteoblasts.
Title: Heat shock protein 22 (HSPB8) limits TGF-?-stimulated migration of osteoblasts.

Results revealed that the apoptosis-suppressing effect of heat shock protein B8 was mediated by the PI3K/Akt pathway in an in vitro model of ischemia.
Title: HspB8 mediates neuroprotection against OGD/R in N2A cells through the phosphoinositide 3-kinase/Akt pathway.

Increased Hsp20 expression protects against OGDR-induced Golgi fragmentation and apoptosis, likely through interaction with Bax and subsequent amelioration of the OGDR-induced elevation in p115 cleavage via the Fas/FasL signaling pathway.
Title: Hsp20 Protects against Oxygen-Glucose Deprivation/Reperfusion-Induced Golgi Fragmentation and Apoptosis through Fas/FasL Pathway.

Translocation of both Hsp22 and iNOS to the mitochondria is necessary for Hsp22-mediated stimulation of oxidative phosphorylation
Title: Heat shock protein 22 (Hsp22) regulates oxidative phosphorylation upon its mitochondrial translocation with the inducible nitric oxide synthase in mammalian heart.

The results of this study strongly suggested that Hspb8 and its alpha-crystallin domain might act as pleiotropic prosurvival factor in the adult hippocampus.
Title: The ? crystallin domain of small heat shock protein b8 (Hspb8) acts as survival and differentiation factor in adult hippocampal neurogenesis.

The following Hspb8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hspb8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19689	NM_030704.3 Latest version!	Mus musculus heat shock protein 8 (Hspb8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hspb8 ( NM_030704.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109629.1 Mus musculus heat shock protein 8 (Hspb8), mRNA.

Price & Availability↑

CloneID	OMu19689
Clone ID Related Accession (Same CDS sequence)	NM_030704.3
Accession Version	NM_030704.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	heat shock protein beta-8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK005052.1 and AI326838.1. On May 8, 2015 this sequence version replaced NM_030704.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK005052.1, BC003971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACG GGCAATTGCC TTTCCCGTGC TCCTACCCAA GCCGCCTGCG CCGAGACCCC 
TTTCGGGACT CACCGCTCTC CTCCCGCCTT CTGGACGATG GCTTTGGCAT GGACCCTTTT 
CCAGACGACT TGACAGCCCC TTGGCCAGAG TGGGCTTTGC CTCGGCTCTC CTCTGCCTGG 
CCTGGAACCC TAAGGTCTGG CATGGTACCC CGAGGGCCTC CAGCCACAGC TAGGTTTGGA 
GTGCCCGCTG AGGGCAGGAG TCCCCCACCC TTCCCTGGGG AGCCTTGGAA AGTGTGTGTC 
AACGTGCACA GCTTCAAGCC GGAAGAGCTG ATGGTAAAGA CCAAGGATGG ATACGTGGAA 
GTTTCAGGCA AACATGAAGA GAAGCAGCAG GAAGGTGGGA TTGTCTCCAA GAACTTCACC 
AAGAAAATAC AGCTCCCTGC AGAAGTGGAT CCAGCCACTG TATTTGCCTC GCTTTCTCCA 
GAGGGCCTGC TCATCATCGA GGCTCCCCAG GTCCCTCCGT ACTCACCCTT TGGAGAGAGC 
AGCTTCAACA ACGAGCTTCC TCAAGACAAC CAGGAAGTCA CCTGTTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109629.1
CDS	386..976
Translation	MADGQLPFPCSYPSRLRRDPFRDSPLSSRLLDDGFGMDPFPDDLTAPWPEWALPRLSSAWPGTLRSGMVPRGPPATARFGVPAEGRSPPPFPGEPWKVCVNVHSFKPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPATVFASLSPEGLLIIEAPQVPPYSPFGESSFNNELPQDNQEVTCS

Target ORF information:

RefSeq Version	NM_030704.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 8 (Hspb8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030704.3

ORF Insert Sequence:

ATGGCTGACG GGCAATTGCC TTTCCCGTGC TCCTACCCAA GCCGCCTGCG CCGAGACCCC 
TTTCGGGACT CACCGCTCTC CTCCCGCCTT CTGGACGATG GCTTTGGCAT GGACCCTTTT 
CCAGACGACT TGACAGCCCC TTGGCCAGAG TGGGCTTTGC CTCGGCTCTC CTCTGCCTGG 
CCTGGAACCC TAAGGTCTGG CATGGTACCC CGAGGGCCTC CAGCCACAGC TAGGTTTGGA 
GTGCCCGCTG AGGGCAGGAG TCCCCCACCC TTCCCTGGGG AGCCTTGGAA AGTGTGTGTC 
AACGTGCACA GCTTCAAGCC GGAAGAGCTG ATGGTAAAGA CCAAGGATGG ATACGTGGAA 
GTTTCAGGCA AACATGAAGA GAAGCAGCAG GAAGGTGGGA TTGTCTCCAA GAACTTCACC 
AAGAAAATAC AGCTCCCTGC AGAAGTGGAT CCAGCCACTG TATTTGCCTC GCTTTCTCCA 
GAGGGCCTGC TCATCATCGA GGCTCCCCAG GTCCCTCCGT ACTCACCCTT TGGAGAGAGC 
AGCTTCAACA ACGAGCTTCC TCAAGACAAC CAGGAAGTCA CCTGTTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A knock-in/knock-out mouse model of HSPB8-associated distal hereditary motor neuropathy and myopathy reveals toxic gain-of-function of mutant Hspb8.
Acta neuropathologica135(1)131-148(2018 Jan)
Bouhy D,Juneja M,Katona I,Holmgren A,Asselbergh B,De Winter V,Hochepied T,Goossens S,Haigh JJ,Libert C,Ceuterick-de Groote C,Irobi J,Weis J,Timmerman V

Heat shock protein 22 (HSPB8) limits TGF-?-stimulated migration of osteoblasts.
Molecular and cellular endocrinology4361-9(2016 Nov)
Yamamoto N,Tokuda H,Kuroyanagi G,Kainuma S,Matsushima-Nishiwaki R,Fujita K,Kozawa O,Otsuka T

HspB8 mediates neuroprotection against OGD/R in N2A cells through the phosphoinositide 3-kinase/Akt pathway.
Brain research164415-21(2016 08)
Hu Z,Yang B,Mo X,Zhou F

Hsp20 Protects against Oxygen-Glucose Deprivation/Reperfusion-Induced Golgi Fragmentation and Apoptosis through Fas/FasL Pathway.
Oxidative medicine and cellular longevity2015606934(2015)
Zhong B,Hu Z,Tan J,Lu T,Lei Q,Chen C,Zeng L