LOC100117168 gene cDNA ORF clone, Nasonia vitripennis(jewel wasp)

mRNA	Protein	Name
XM_008219351.2	XP_008217573.1	glycogen debranching enzyme isoform X2
XM_016986669.1	XP_016842158.1	glycogen debranching enzyme isoform X1
XM_008219356.2	XP_008217578.1	glycogen debranching enzyme isoform X5
XM_008219358.2	XP_008217580.1	glycogen debranching enzyme isoform X5
XM_016986671.1	XP_016842160.1	glycogen debranching enzyme isoform X5
XM_016986668.1	XP_016842157.1	glycogen debranching enzyme isoform X1
XM_016986670.1	XP_016842159.1	glycogen debranching enzyme isoform X1
XM_008219355.2	XP_008217577.1	glycogen debranching enzyme isoform X4
XM_008219357.2	XP_008217579.1	glycogen debranching enzyme isoform X5
XM_016986672.1	XP_016842161.1	glycogen debranching enzyme isoform X5
XM_016986668.2	XP_016842157.1	glycogen debranching enzyme
XM_008219354.2	XP_008217576.1	glycogen debranching enzyme isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONd18429	XM_016986670.1 Latest version!	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ONd18429	XM_016986668.2 Latest version!	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ONd18429	XM_016986669.1 Latest version!	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ONd11608	XM_008219351.2 Latest version!	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ONd11609	XM_008219354.2 Latest version!	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ONd11610	XM_008219355.2 Latest version!	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ONd11611	XM_016986671.1 Latest version!	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ONd11611	XM_008219356.2 Latest version!	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ONd11611	XM_008219357.2 Latest version!	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ONd11611	XM_008219358.2 Latest version!	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ONd11611	XM_016986672.1 Latest version!	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ONd18429	XM_016986668.1	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

LOC100117168 ( XM_016986669.1 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_016842158.1 Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONd18429
Clone ID Related Accession (Same CDS sequence)	XM_016986669.1 , XM_016986668.1 , XM_016986670.1 , XM_016986668.2
Accession Version	XM_016986669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-25
Organism	Nasonia vitripennis(jewel wasp)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001815014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGCTC TGCAGAATCT AGTGTGTGAC TCCACACGAC AATTATTCAA GAAGATAGGG 
TATTTCTTTC ACCGCTGCTT GACCTTATTT TGGTCTTATC TTACCAATGT GAAATCAACA 
TTTCTAAATT TACAGTTACA TCTGCAACGT GCAATCGACG ATTCCAAAAT TACACCCAAA 
AAGAAGACCA TGGAAAATCT CGGCTTCTCT GCGGATACCC AGAACGACGA CCAAGTGCGA 
GTACTGACTC TGAATAAGGG AGAGCACCAG GATGGGACCC TGTACAAGCT TAAAAAAGGA 
TGGGTCGTGG AGTTCAGATT AGGGGCATCG CTTCTGGGCC ACAATCTCAA CATATTCACT 
AATCATCCTC TACTCGTCGG CAACCCGTTC GAGAGGCGCA CGTACTATCA GCTGCCATGG 
AATCACGAGT CAGCTTTCAT ACGCGTCTCG CTGGCCGGCT CTTTTCATTT TTATCTGGCA 
GAAGCGAGTG GAGAGGACGC CGGAAAAGTG ATAGCTTCCG GCTACTTGTT GGTCGATCCG 
GAGCTAACCG TGGGCGTCAA CAACCAGCCG CTGCCGCTAG ACTGCATACA ATGCCAGACG 
GTCCTCTCAA AGCTCATGGG ACCCTTCTCA ACGTGGGAGG AGAAGCTCAG GGTCTCTCGC 
AAGTCAGGCT ACAACATGAT CCACTTCACA CCGATACAGG CGATAGGAGA CTCAAAATCA 
GCTTACAGCC TCAGCGATCA GCTGAAGCTC AACCCCTCGT TCAATGATGC ACCGAACAAA 
GAGACCAGCT TCGACGACGT CGAGAAGTTG ATTACAAAAA TGAGAGAAGA GTGGAACACT 
GTGAGCATTT GCGACATAGT TTTGAATCAC ACGGCGAACG AGAGTCCATT TTTGATAAAC 
AATCCCGAAT GTAGTTACAA CTGCAACAAC TGTCCACATC TCCGTCCGTC TTATCTTTTG 
GATGCCATTT TATTCGAGTT GACAATACGA GTGTCGTCCG GAGAATGGGA GCACAAAGGA 
ATTCCTCCCG TCGTCGAGAC TGAAGAGCAC TTGAACGCAA TCCGTCATGC TCTGCACACT 
TACTTTCTAC CTCTGGCGAA AATTCACGAA CTGTATACTG TAGATATCAA CGAGATCGTG 
TCCGAGTTTT TGAACATTGC TCGCAACCAG ATACCGCAAG ACGAGCCACC CGAAACCCAT 
AACGAAAGCA TCGTTATCAT AAGGGATCCA GAATATCGCC GCTTAAAGTC CACTATAGAT 
ATGCAGCTTG CTTTGAAGAT CTACAATATT TATAGACCCG ATTGTTTTGA CGAGGACTCA 
CGACTGAAAC GCTGCGCGGA AGAACTGAGA AACAAGTTGC AGGAGTTAAA CGATGCCGCA 
ACTAACGAAA TCCAAAATCA TCTCAATGCC GCGGTTGAAA ACTCGGTAGC CGGCATTCGA 
TACTTCAGAG TCCAAGCAGA TGGGCCAAGA CAGAAAGAGA TAAGCGAAAG AAATCCACTT 
GTTCCTAGGT ATTTCACGGA TTACGGAAGT CCTAGATCGT TTGTGGAAAG AGAAGACATC 
ATGTACTCGA ATTCTGGATG TTACTTGATG GCTCACAACG GCTGGGTCAT GAACAGCGAT 
CCCTTGAAAA ACTTTGCCGA TGCTGACTCT CATGTTTACA TAAGAAGGGA ATTGATTGCG 
TGGGGCGATA GTGTCAAATT AAGATATGGC GAAAAACCGG AAGATTGCCC TGCTCTTTGG 
GAACGTATGA CGACATATGT TCAGCAAATG GCTAAAATCT TCGACGGCGT CAGACTCGAC 
AATTGTCACT CGACCCCTAT TCCAGTAGCA GAACACATGT TGGATGCTGC TCGTCAAGTA 
AGACCAGATT TGTACGTTGC CGCTGAACTC TTCACCAACT CCGATCAGAA AGATAATATT 
TTCGTCAATC GTCTTGGAAT CACATCGTTG ATAAGAGAGG CCATGTCAGC GTGGGACAGC 
CATGAGGAAG GCCGTCTGGT ATACCGCTAT GGTGGCGAGC CCGTAGGTGC GTTTATCCAG 
GCTCGACATC GTCCTCTGGC ACCGAGTATC GCGCATGCTT TATTCCTTGA TCAGACGCAT 
GACAACCCGA GCCCCATCGA GAAGCGTAGC GTATTTGACT TATTGCCCAG TGCCGCACTG 
GTCTCCATGG CCTGCTGTGC TAGTGGCAGT AATCGCGGTT ACGACGAGCT CGTGCCTCAT 
CATATTCACG TTGTCGATGA AGACAGACAA TATACTTCTT GGACAGATAA GGAAGGACTG 
GCAAAGGAAA ATGCAAAGTA TGTTAGCAAA AAATCGGGAA TCATTTCTGC TAAAAAGGCT 
CTCAATGATC TCCATTTTTG GCTGGGACAG AACTCCTTTT CTCAAGTTTT TGTCGATCAA 
ATGGATGTTG ATGTGGTAGC GGTTACAAGA CATTCACCGA CCACTCACGA GAGCGTCGTA 
CTCGTGGCTT TTACGGCATT CAAGCACCCG GATCCCAATG CTAGCGATTT GAGAAGACAT 
GTTAGACCTT TGAGGGTAGA AGGCGTAGTA GATGAAATTA TTCTTGAAGC ATCCCTCGCG 
CACGGAGAAA AAGAAAATGG TATCCCTCCG TTCTCTAATC CGAAGAAATA CGTAAAGAAC 
GAAGACTACA TAAACGGTTA CTCTGAGTAC TTGGTGGACG TCAAGGAGCA CATTCAAATC 
TGTGATTCTA CAAAAGTTGA AAAGGTCGAT TCCGGAGATC CTAAAATAAC GCAATTGAAC 
TTTATTAATT TCCAGCCCGG ATCAATAATC ACTATTCGGG TGTCACTCCA CGCAAACATA 
AAGCCAGCTC TTGAATCCAT CAATGACCTT ATCTCGACCC TCACATCTCC GAACAATTCG 
GAATTGCGTG CAATTGTTTC GCGCATGAAC TTTGCGGACC TTAACAAAGC ACTTTATAGG 
TGTGATCAAG AAGAGCGAGA TACGACTGCC AATAAATTTG GAGTTTATGA TATTCCCGGT 
TATGGCCCAC TAGTTTACGC TGGTTTGCAA GGTGTAATTT CATTGCTTGC TGATATCAGA 
TCGAACAACG ATCTTGGTCA TCCACTATGT GGCAACCTTC GTCAAGGCAA TTGGTTGATA 
GATTATACTT GGCAACGTTT GAAGGAAGAT GAAGGTACTG CAGCTCTTGG AGAGTGGCTG 
GAGCAGGCCT GCGAACCATT CAAGCTAATT CCTCGTTATT TAGTGCCCAG TTACTTTGAC 
GTGATCATCG TTAACGTGTA TTCTACTCTC TTAGATCAAT GCTTTAATCT CATGTCTAGC 
TTTGTGAAAG ATGGAACCGA TTTCACCAGA CTGCTGTCAC TAGTATCTGT GCAAGTTGGC 
GGTATAATAA AATCAGCGCA ATTGCCAGAT TTATCACCGA ACCTAGATCC ACCGAAACCT 
AGCATACGAG AGGTGGATGG CAAATCAGAA CAGGAATGTT TGACCATGTC TGCTGGACTA 
CCACACTTTA CAGTAGGATA CATGAGAAAC TGGGGACGAG ATACGTTTAT CGCTTTGAGA 
GGTCTGTTCT TATTAACCGG CAGACATGCG GAGGCTCGAT TCATCATTCT GGGATACGCC 
GCTACGCTTC GTCACGGTTT GATACCAAAT CTCTTGGACA GAGGCGTCAA CGCCAGATTC 
AATTGCCGAG ATGCCGTTTG GTGGTGGTTG TACACTATTC AATGCTATGT CAAAGAAGTC 
CCAAATGGCT TAAAACTTCT GTCGGACAAA GTATCTAGAT TGTTCCCTAC TGACGATTCG 
GACGCTCTTC CAGCCGGAGA AGTGGATGAA ACACTGTCGG ATGTCATGCA GGAAGCACTG 
ACAGTTCACT TTCAAGGTCT GTGCTTCCGT GAGCGCAACG CCGGTAAGCG CATCGACGAG 
CACATGACTG ATCGTGGATT CAACAATCAG ATAGGTGTGC ATCCAGAGAC GGGCTTTGTA 
TTTGGCGGTA ACGAGGCCAA TTGCGGCACT TGGATGGACA AGATGGGCTC CTCGGAAAAG 
GCAGGAAACA AAGGCAAACC TGCCACTCCC AGAGACGGAT CTGCGGTTGA AATTATTGGG 
CTCTGTCACA GCACTCTCAA TTTTCTGGCA GAATTGTACA AACAGAATCT ATTTCCATAT 
GGTAGCGTGC AGCGGAAAAA TCGCGATGGC TCAACGATAA CTTGGAGTTA CAAACAGTGG 
GCGGATAAGA TAAAGGAGAG TTTCGATACT TACTTCTATG TGAACGAGAA ACCTACCGAT 
GGTGAATTAC GACCGGACTT GATTCATCGA CGAGGAATTT ACAAAGATAC TCACGGAGCC 
TCACAACCGT GGGCAGATTA TCAATTGCGA CCTAACTTCC CAGTGGCTAT GGTTGCTTCG 
CCGAACCTAT TCAAGCCCGA GCACGCCTGG CTGGCATTAA AGAAGGCCGA AGAAATCCTC 
CTTGGTCCCC TAGGCATGAA AACCCTAGAT CCCGCTGACT GGGCCTACGA TGGCTTCTAC 
AACAATGCCA ACGACTCGAA TGACCCGAAA GTAGCTCACG GATGGAATTA TCACCAGGGA 
CCAGAGTGGC TATGGCCGAT CGGCTACTTT TTGCGAGCGC GCTTGCACTA CGCGCCCCTC 
GTTGGTGGCG AAGAGGAGTT AAAACGGACT ATCGCCTCGA CCGAAGCTAT CATCTCGAGA 
CATCTGACCG AAGCTTCAAA CAACCACTGG CGTGGACTTC CCGAGTTAAC CAATCGCGAG 
GGCGAGTATT GCGCTGATAG CTGTCGCACT CAGGCCTGGA GCGCTTCCGC AATTGTCGAG 
GTCCTCCACG ATTTGGACAA GCTGAAAAAG GATCTGAAGT TTGACGACGA GCAGGTCCTC 
ACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016842158.1
CDS	343..5211
Translation	MVALQNLVCDSTRQLFKKIGYFFHRCLTLFWSYLTNVKSTFLNLQLHLQRAIDDSKITPKKKTMENLGFSADTQNDDQVRVLTLNKGEHQDGTLYKLKKGWVVEFRLGASLLGHNLNIFTNHPLLVGNPFERRTYYQLPWNHESAFIRVSLAGSFHFYLAEASGEDAGKVIASGYLLVDPELTVGVNNQPLPLDCIQCQTVLSKLMGPFSTWEEKLRVSRKSGYNMIHFTPIQAIGDSKSAYSLSDQLKLNPSFNDAPNKETSFDDVEKLITKMREEWNTVSICDIVLNHTANESPFLINNPECSYNCNNCPHLRPSYLLDAILFELTIRVSSGEWEHKGIPPVVETEEHLNAIRHALHTYFLPLAKIHELYTVDINEIVSEFLNIARNQIPQDEPPETHNESIVIIRDPEYRRLKSTIDMQLALKIYNIYRPDCFDEDSRLKRCAEELRNKLQELNDAATNEIQNHLNAAVENSVAGIRYFRVQADGPRQKEISERNPLVPRYFTDYGSPRSFVEREDIMYSNSGCYLMAHNGWVMNSDPLKNFADADSHVYIRRELIAWGDSVKLRYGEKPEDCPALWERMTTYVQQMAKIFDGVRLDNCHSTPIPVAEHMLDAARQVRPDLYVAAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFIQARHRPLAPSIAHALFLDQTHDNPSPIEKRSVFDLLPSAALVSMACCASGSNRGYDELVPHHIHVVDEDRQYTSWTDKEGLAKENAKYVSKKSGIISAKKALNDLHFWLGQNSFSQVFVDQMDVDVVAVTRHSPTTHESVVLVAFTAFKHPDPNASDLRRHVRPLRVEGVVDEIILEASLAHGEKENGIPPFSNPKKYVKNEDYINGYSEYLVDVKEHIQICDSTKVEKVDSGDPKITQLNFINFQPGSIITIRVSLHANIKPALESINDLISTLTSPNNSELRAIVSRMNFADLNKALYRCDQEERDTTANKFGVYDIPGYGPLVYAGLQGVISLLADIRSNNDLGHPLCGNLRQGNWLIDYTWQRLKEDEGTAALGEWLEQACEPFKLIPRYLVPSYFDVIIVNVYSTLLDQCFNLMSSFVKDGTDFTRLLSLVSVQVGGIIKSAQLPDLSPNLDPPKPSIREVDGKSEQECLTMSAGLPHFTVGYMRNWGRDTFIALRGLFLLTGRHAEARFIILGYAATLRHGLIPNLLDRGVNARFNCRDAVWWWLYTIQCYVKEVPNGLKLLSDKVSRLFPTDDSDALPAGEVDETLSDVMQEALTVHFQGLCFRERNAGKRIDEHMTDRGFNNQIGVHPETGFVFGGNEANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIIGLCHSTLNFLAELYKQNLFPYGSVQRKNRDGSTITWSYKQWADKIKESFDTYFYVNEKPTDGELRPDLIHRRGIYKDTHGASQPWADYQLRPNFPVAMVASPNLFKPEHAWLALKKAEEILLGPLGMKTLDPADWAYDGFYNNANDSNDPKVAHGWNYHQGPEWLWPIGYFLRARLHYAPLVGGEEELKRTIASTEAIISRHLTEASNNHWRGLPELTNREGEYCADSCRTQAWSASAIVEVLHDLDKLKKDLKFDDEQVLTN

Target ORF information:

RefSeq Version	XM_016986669.1
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016986669.1

ORF Insert Sequence:

ATGGTTGCTC TGCAGAATCT AGTGTGTGAC TCCACACGAC AATTATTCAA GAAGATAGGG 
TATTTCTTTC ACCGCTGCTT GACCTTATTT TGGTCTTATC TTACCAATGT GAAATCAACA 
TTTCTAAATT TACAGTTACA TCTGCAACGT GCAATCGACG ATTCCAAAAT TACACCCAAA 
AAGAAGACCA TGGAAAATCT CGGCTTCTCT GCGGATACCC AGAACGACGA CCAAGTGCGA 
GTACTGACTC TGAATAAGGG AGAGCACCAG GATGGGACCC TGTACAAGCT TAAAAAAGGA 
TGGGTCGTGG AGTTCAGATT AGGGGCATCG CTTCTGGGCC ACAATCTCAA CATATTCACT 
AATCATCCTC TACTCGTCGG CAACCCGTTC GAGAGGCGCA CGTACTATCA GCTGCCATGG 
AATCACGAGT CAGCTTTCAT ACGCGTCTCG CTGGCCGGCT CTTTTCATTT TTATCTGGCA 
GAAGCGAGTG GAGAGGACGC CGGAAAAGTG ATAGCTTCCG GCTACTTGTT GGTCGATCCG 
GAGCTAACCG TGGGCGTCAA CAACCAGCCG CTGCCGCTAG ACTGCATACA ATGCCAGACG 
GTCCTCTCAA AGCTCATGGG ACCCTTCTCA ACGTGGGAGG AGAAGCTCAG GGTCTCTCGC 
AAGTCAGGCT ACAACATGAT CCACTTCACA CCGATACAGG CGATAGGAGA CTCAAAATCA 
GCTTACAGCC TCAGCGATCA GCTGAAGCTC AACCCCTCGT TCAATGATGC ACCGAACAAA 
GAGACCAGCT TCGACGACGT CGAGAAGTTG ATTACAAAAA TGAGAGAAGA GTGGAACACT 
GTGAGCATTT GCGACATAGT TTTGAATCAC ACGGCGAACG AGAGTCCATT TTTGATAAAC 
AATCCCGAAT GTAGTTACAA CTGCAACAAC TGTCCACATC TCCGTCCGTC TTATCTTTTG 
GATGCCATTT TATTCGAGTT GACAATACGA GTGTCGTCCG GAGAATGGGA GCACAAAGGA 
ATTCCTCCCG TCGTCGAGAC TGAAGAGCAC TTGAACGCAA TCCGTCATGC TCTGCACACT 
TACTTTCTAC CTCTGGCGAA AATTCACGAA CTGTATACTG TAGATATCAA CGAGATCGTG 
TCCGAGTTTT TGAACATTGC TCGCAACCAG ATACCGCAAG ACGAGCCACC CGAAACCCAT 
AACGAAAGCA TCGTTATCAT AAGGGATCCA GAATATCGCC GCTTAAAGTC CACTATAGAT 
ATGCAGCTTG CTTTGAAGAT CTACAATATT TATAGACCCG ATTGTTTTGA CGAGGACTCA 
CGACTGAAAC GCTGCGCGGA AGAACTGAGA AACAAGTTGC AGGAGTTAAA CGATGCCGCA 
ACTAACGAAA TCCAAAATCA TCTCAATGCC GCGGTTGAAA ACTCGGTAGC CGGCATTCGA 
TACTTCAGAG TCCAAGCAGA TGGGCCAAGA CAGAAAGAGA TAAGCGAAAG AAATCCACTT 
GTTCCTAGGT ATTTCACGGA TTACGGAAGT CCTAGATCGT TTGTGGAAAG AGAAGACATC 
ATGTACTCGA ATTCTGGATG TTACTTGATG GCTCACAACG GCTGGGTCAT GAACAGCGAT 
CCCTTGAAAA ACTTTGCCGA TGCTGACTCT CATGTTTACA TAAGAAGGGA ATTGATTGCG 
TGGGGCGATA GTGTCAAATT AAGATATGGC GAAAAACCGG AAGATTGCCC TGCTCTTTGG 
GAACGTATGA CGACATATGT TCAGCAAATG GCTAAAATCT TCGACGGCGT CAGACTCGAC 
AATTGTCACT CGACCCCTAT TCCAGTAGCA GAACACATGT TGGATGCTGC TCGTCAAGTA 
AGACCAGATT TGTACGTTGC CGCTGAACTC TTCACCAACT CCGATCAGAA AGATAATATT 
TTCGTCAATC GTCTTGGAAT CACATCGTTG ATAAGAGAGG CCATGTCAGC GTGGGACAGC 
CATGAGGAAG GCCGTCTGGT ATACCGCTAT GGTGGCGAGC CCGTAGGTGC GTTTATCCAG 
GCTCGACATC GTCCTCTGGC ACCGAGTATC GCGCATGCTT TATTCCTTGA TCAGACGCAT 
GACAACCCGA GCCCCATCGA GAAGCGTAGC GTATTTGACT TATTGCCCAG TGCCGCACTG 
GTCTCCATGG CCTGCTGTGC TAGTGGCAGT AATCGCGGTT ACGACGAGCT CGTGCCTCAT 
CATATTCACG TTGTCGATGA AGACAGACAA TATACTTCTT GGACAGATAA GGAAGGACTG 
GCAAAGGAAA ATGCAAAGTA TGTTAGCAAA AAATCGGGAA TCATTTCTGC TAAAAAGGCT 
CTCAATGATC TCCATTTTTG GCTGGGACAG AACTCCTTTT CTCAAGTTTT TGTCGATCAA 
ATGGATGTTG ATGTGGTAGC GGTTACAAGA CATTCACCGA CCACTCACGA GAGCGTCGTA 
CTCGTGGCTT TTACGGCATT CAAGCACCCG GATCCCAATG CTAGCGATTT GAGAAGACAT 
GTTAGACCTT TGAGGGTAGA AGGCGTAGTA GATGAAATTA TTCTTGAAGC ATCCCTCGCG 
CACGGAGAAA AAGAAAATGG TATCCCTCCG TTCTCTAATC CGAAGAAATA CGTAAAGAAC 
GAAGACTACA TAAACGGTTA CTCTGAGTAC TTGGTGGACG TCAAGGAGCA CATTCAAATC 
TGTGATTCTA CAAAAGTTGA AAAGGTCGAT TCCGGAGATC CTAAAATAAC GCAATTGAAC 
TTTATTAATT TCCAGCCCGG ATCAATAATC ACTATTCGGG TGTCACTCCA CGCAAACATA 
AAGCCAGCTC TTGAATCCAT CAATGACCTT ATCTCGACCC TCACATCTCC GAACAATTCG 
GAATTGCGTG CAATTGTTTC GCGCATGAAC TTTGCGGACC TTAACAAAGC ACTTTATAGG 
TGTGATCAAG AAGAGCGAGA TACGACTGCC AATAAATTTG GAGTTTATGA TATTCCCGGT 
TATGGCCCAC TAGTTTACGC TGGTTTGCAA GGTGTAATTT CATTGCTTGC TGATATCAGA 
TCGAACAACG ATCTTGGTCA TCCACTATGT GGCAACCTTC GTCAAGGCAA TTGGTTGATA 
GATTATACTT GGCAACGTTT GAAGGAAGAT GAAGGTACTG CAGCTCTTGG AGAGTGGCTG 
GAGCAGGCCT GCGAACCATT CAAGCTAATT CCTCGTTATT TAGTGCCCAG TTACTTTGAC 
GTGATCATCG TTAACGTGTA TTCTACTCTC TTAGATCAAT GCTTTAATCT CATGTCTAGC 
TTTGTGAAAG ATGGAACCGA TTTCACCAGA CTGCTGTCAC TAGTATCTGT GCAAGTTGGC 
GGTATAATAA AATCAGCGCA ATTGCCAGAT TTATCACCGA ACCTAGATCC ACCGAAACCT 
AGCATACGAG AGGTGGATGG CAAATCAGAA CAGGAATGTT TGACCATGTC TGCTGGACTA 
CCACACTTTA CAGTAGGATA CATGAGAAAC TGGGGACGAG ATACGTTTAT CGCTTTGAGA 
GGTCTGTTCT TATTAACCGG CAGACATGCG GAGGCTCGAT TCATCATTCT GGGATACGCC 
GCTACGCTTC GTCACGGTTT GATACCAAAT CTCTTGGACA GAGGCGTCAA CGCCAGATTC 
AATTGCCGAG ATGCCGTTTG GTGGTGGTTG TACACTATTC AATGCTATGT CAAAGAAGTC 
CCAAATGGCT TAAAACTTCT GTCGGACAAA GTATCTAGAT TGTTCCCTAC TGACGATTCG 
GACGCTCTTC CAGCCGGAGA AGTGGATGAA ACACTGTCGG ATGTCATGCA GGAAGCACTG 
ACAGTTCACT TTCAAGGTCT GTGCTTCCGT GAGCGCAACG CCGGTAAGCG CATCGACGAG 
CACATGACTG ATCGTGGATT CAACAATCAG ATAGGTGTGC ATCCAGAGAC GGGCTTTGTA 
TTTGGCGGTA ACGAGGCCAA TTGCGGCACT TGGATGGACA AGATGGGCTC CTCGGAAAAG 
GCAGGAAACA AAGGCAAACC TGCCACTCCC AGAGACGGAT CTGCGGTTGA AATTATTGGG 
CTCTGTCACA GCACTCTCAA TTTTCTGGCA GAATTGTACA AACAGAATCT ATTTCCATAT 
GGTAGCGTGC AGCGGAAAAA TCGCGATGGC TCAACGATAA CTTGGAGTTA CAAACAGTGG 
GCGGATAAGA TAAAGGAGAG TTTCGATACT TACTTCTATG TGAACGAGAA ACCTACCGAT 
GGTGAATTAC GACCGGACTT GATTCATCGA CGAGGAATTT ACAAAGATAC TCACGGAGCC 
TCACAACCGT GGGCAGATTA TCAATTGCGA CCTAACTTCC CAGTGGCTAT GGTTGCTTCG 
CCGAACCTAT TCAAGCCCGA GCACGCCTGG CTGGCATTAA AGAAGGCCGA AGAAATCCTC 
CTTGGTCCCC TAGGCATGAA AACCCTAGAT CCCGCTGACT GGGCCTACGA TGGCTTCTAC 
AACAATGCCA ACGACTCGAA TGACCCGAAA GTAGCTCACG GATGGAATTA TCACCAGGGA 
CCAGAGTGGC TATGGCCGAT CGGCTACTTT TTGCGAGCGC GCTTGCACTA CGCGCCCCTC 
GTTGGTGGCG AAGAGGAGTT AAAACGGACT ATCGCCTCGA CCGAAGCTAT CATCTCGAGA 
CATCTGACCG AAGCTTCAAA CAACCACTGG CGTGGACTTC CCGAGTTAAC CAATCGCGAG 
GGCGAGTATT GCGCTGATAG CTGTCGCACT CAGGCCTGGA GCGCTTCCGC AATTGTCGAG 
GTCCTCCACG ATTTGGACAA GCTGAAAAAG GATCTGAAGT TTGACGACGA GCAGGTCCTC 
ACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100117168 ( XM_016986668.1 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_016842157.1 Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONd18429
Clone ID Related Accession (Same CDS sequence)	XM_016986669.1 , XM_016986668.1 , XM_016986670.1 , XM_016986668.2
Accession Version	XM_016986668.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-25
Organism	Nasonia vitripennis(jewel wasp)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001815014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGCTC TGCAGAATCT AGTGTGTGAC TCCACACGAC AATTATTCAA GAAGATAGGG 
TATTTCTTTC ACCGCTGCTT GACCTTATTT TGGTCTTATC TTACCAATGT GAAATCAACA 
TTTCTAAATT TACAGTTACA TCTGCAACGT GCAATCGACG ATTCCAAAAT TACACCCAAA 
AAGAAGACCA TGGAAAATCT CGGCTTCTCT GCGGATACCC AGAACGACGA CCAAGTGCGA 
GTACTGACTC TGAATAAGGG AGAGCACCAG GATGGGACCC TGTACAAGCT TAAAAAAGGA 
TGGGTCGTGG AGTTCAGATT AGGGGCATCG CTTCTGGGCC ACAATCTCAA CATATTCACT 
AATCATCCTC TACTCGTCGG CAACCCGTTC GAGAGGCGCA CGTACTATCA GCTGCCATGG 
AATCACGAGT CAGCTTTCAT ACGCGTCTCG CTGGCCGGCT CTTTTCATTT TTATCTGGCA 
GAAGCGAGTG GAGAGGACGC CGGAAAAGTG ATAGCTTCCG GCTACTTGTT GGTCGATCCG 
GAGCTAACCG TGGGCGTCAA CAACCAGCCG CTGCCGCTAG ACTGCATACA ATGCCAGACG 
GTCCTCTCAA AGCTCATGGG ACCCTTCTCA ACGTGGGAGG AGAAGCTCAG GGTCTCTCGC 
AAGTCAGGCT ACAACATGAT CCACTTCACA CCGATACAGG CGATAGGAGA CTCAAAATCA 
GCTTACAGCC TCAGCGATCA GCTGAAGCTC AACCCCTCGT TCAATGATGC ACCGAACAAA 
GAGACCAGCT TCGACGACGT CGAGAAGTTG ATTACAAAAA TGAGAGAAGA GTGGAACACT 
GTGAGCATTT GCGACATAGT TTTGAATCAC ACGGCGAACG AGAGTCCATT TTTGATAAAC 
AATCCCGAAT GTAGTTACAA CTGCAACAAC TGTCCACATC TCCGTCCGTC TTATCTTTTG 
GATGCCATTT TATTCGAGTT GACAATACGA GTGTCGTCCG GAGAATGGGA GCACAAAGGA 
ATTCCTCCCG TCGTCGAGAC TGAAGAGCAC TTGAACGCAA TCCGTCATGC TCTGCACACT 
TACTTTCTAC CTCTGGCGAA AATTCACGAA CTGTATACTG TAGATATCAA CGAGATCGTG 
TCCGAGTTTT TGAACATTGC TCGCAACCAG ATACCGCAAG ACGAGCCACC CGAAACCCAT 
AACGAAAGCA TCGTTATCAT AAGGGATCCA GAATATCGCC GCTTAAAGTC CACTATAGAT 
ATGCAGCTTG CTTTGAAGAT CTACAATATT TATAGACCCG ATTGTTTTGA CGAGGACTCA 
CGACTGAAAC GCTGCGCGGA AGAACTGAGA AACAAGTTGC AGGAGTTAAA CGATGCCGCA 
ACTAACGAAA TCCAAAATCA TCTCAATGCC GCGGTTGAAA ACTCGGTAGC CGGCATTCGA 
TACTTCAGAG TCCAAGCAGA TGGGCCAAGA CAGAAAGAGA TAAGCGAAAG AAATCCACTT 
GTTCCTAGGT ATTTCACGGA TTACGGAAGT CCTAGATCGT TTGTGGAAAG AGAAGACATC 
ATGTACTCGA ATTCTGGATG TTACTTGATG GCTCACAACG GCTGGGTCAT GAACAGCGAT 
CCCTTGAAAA ACTTTGCCGA TGCTGACTCT CATGTTTACA TAAGAAGGGA ATTGATTGCG 
TGGGGCGATA GTGTCAAATT AAGATATGGC GAAAAACCGG AAGATTGCCC TGCTCTTTGG 
GAACGTATGA CGACATATGT TCAGCAAATG GCTAAAATCT TCGACGGCGT CAGACTCGAC 
AATTGTCACT CGACCCCTAT TCCAGTAGCA GAACACATGT TGGATGCTGC TCGTCAAGTA 
AGACCAGATT TGTACGTTGC CGCTGAACTC TTCACCAACT CCGATCAGAA AGATAATATT 
TTCGTCAATC GTCTTGGAAT CACATCGTTG ATAAGAGAGG CCATGTCAGC GTGGGACAGC 
CATGAGGAAG GCCGTCTGGT ATACCGCTAT GGTGGCGAGC CCGTAGGTGC GTTTATCCAG 
GCTCGACATC GTCCTCTGGC ACCGAGTATC GCGCATGCTT TATTCCTTGA TCAGACGCAT 
GACAACCCGA GCCCCATCGA GAAGCGTAGC GTATTTGACT TATTGCCCAG TGCCGCACTG 
GTCTCCATGG CCTGCTGTGC TAGTGGCAGT AATCGCGGTT ACGACGAGCT CGTGCCTCAT 
CATATTCACG TTGTCGATGA AGACAGACAA TATACTTCTT GGACAGATAA GGAAGGACTG 
GCAAAGGAAA ATGCAAAGTA TGTTAGCAAA AAATCGGGAA TCATTTCTGC TAAAAAGGCT 
CTCAATGATC TCCATTTTTG GCTGGGACAG AACTCCTTTT CTCAAGTTTT TGTCGATCAA 
ATGGATGTTG ATGTGGTAGC GGTTACAAGA CATTCACCGA CCACTCACGA GAGCGTCGTA 
CTCGTGGCTT TTACGGCATT CAAGCACCCG GATCCCAATG CTAGCGATTT GAGAAGACAT 
GTTAGACCTT TGAGGGTAGA AGGCGTAGTA GATGAAATTA TTCTTGAAGC ATCCCTCGCG 
CACGGAGAAA AAGAAAATGG TATCCCTCCG TTCTCTAATC CGAAGAAATA CGTAAAGAAC 
GAAGACTACA TAAACGGTTA CTCTGAGTAC TTGGTGGACG TCAAGGAGCA CATTCAAATC 
TGTGATTCTA CAAAAGTTGA AAAGGTCGAT TCCGGAGATC CTAAAATAAC GCAATTGAAC 
TTTATTAATT TCCAGCCCGG ATCAATAATC ACTATTCGGG TGTCACTCCA CGCAAACATA 
AAGCCAGCTC TTGAATCCAT CAATGACCTT ATCTCGACCC TCACATCTCC GAACAATTCG 
GAATTGCGTG CAATTGTTTC GCGCATGAAC TTTGCGGACC TTAACAAAGC ACTTTATAGG 
TGTGATCAAG AAGAGCGAGA TACGACTGCC AATAAATTTG GAGTTTATGA TATTCCCGGT 
TATGGCCCAC TAGTTTACGC TGGTTTGCAA GGTGTAATTT CATTGCTTGC TGATATCAGA 
TCGAACAACG ATCTTGGTCA TCCACTATGT GGCAACCTTC GTCAAGGCAA TTGGTTGATA 
GATTATACTT GGCAACGTTT GAAGGAAGAT GAAGGTACTG CAGCTCTTGG AGAGTGGCTG 
GAGCAGGCCT GCGAACCATT CAAGCTAATT CCTCGTTATT TAGTGCCCAG TTACTTTGAC 
GTGATCATCG TTAACGTGTA TTCTACTCTC TTAGATCAAT GCTTTAATCT CATGTCTAGC 
TTTGTGAAAG ATGGAACCGA TTTCACCAGA CTGCTGTCAC TAGTATCTGT GCAAGTTGGC 
GGTATAATAA AATCAGCGCA ATTGCCAGAT TTATCACCGA ACCTAGATCC ACCGAAACCT 
AGCATACGAG AGGTGGATGG CAAATCAGAA CAGGAATGTT TGACCATGTC TGCTGGACTA 
CCACACTTTA CAGTAGGATA CATGAGAAAC TGGGGACGAG ATACGTTTAT CGCTTTGAGA 
GGTCTGTTCT TATTAACCGG CAGACATGCG GAGGCTCGAT TCATCATTCT GGGATACGCC 
GCTACGCTTC GTCACGGTTT GATACCAAAT CTCTTGGACA GAGGCGTCAA CGCCAGATTC 
AATTGCCGAG ATGCCGTTTG GTGGTGGTTG TACACTATTC AATGCTATGT CAAAGAAGTC 
CCAAATGGCT TAAAACTTCT GTCGGACAAA GTATCTAGAT TGTTCCCTAC TGACGATTCG 
GACGCTCTTC CAGCCGGAGA AGTGGATGAA ACACTGTCGG ATGTCATGCA GGAAGCACTG 
ACAGTTCACT TTCAAGGTCT GTGCTTCCGT GAGCGCAACG CCGGTAAGCG CATCGACGAG 
CACATGACTG ATCGTGGATT CAACAATCAG ATAGGTGTGC ATCCAGAGAC GGGCTTTGTA 
TTTGGCGGTA ACGAGGCCAA TTGCGGCACT TGGATGGACA AGATGGGCTC CTCGGAAAAG 
GCAGGAAACA AAGGCAAACC TGCCACTCCC AGAGACGGAT CTGCGGTTGA AATTATTGGG 
CTCTGTCACA GCACTCTCAA TTTTCTGGCA GAATTGTACA AACAGAATCT ATTTCCATAT 
GGTAGCGTGC AGCGGAAAAA TCGCGATGGC TCAACGATAA CTTGGAGTTA CAAACAGTGG 
GCGGATAAGA TAAAGGAGAG TTTCGATACT TACTTCTATG TGAACGAGAA ACCTACCGAT 
GGTGAATTAC GACCGGACTT GATTCATCGA CGAGGAATTT ACAAAGATAC TCACGGAGCC 
TCACAACCGT GGGCAGATTA TCAATTGCGA CCTAACTTCC CAGTGGCTAT GGTTGCTTCG 
CCGAACCTAT TCAAGCCCGA GCACGCCTGG CTGGCATTAA AGAAGGCCGA AGAAATCCTC 
CTTGGTCCCC TAGGCATGAA AACCCTAGAT CCCGCTGACT GGGCCTACGA TGGCTTCTAC 
AACAATGCCA ACGACTCGAA TGACCCGAAA GTAGCTCACG GATGGAATTA TCACCAGGGA 
CCAGAGTGGC TATGGCCGAT CGGCTACTTT TTGCGAGCGC GCTTGCACTA CGCGCCCCTC 
GTTGGTGGCG AAGAGGAGTT AAAACGGACT ATCGCCTCGA CCGAAGCTAT CATCTCGAGA 
CATCTGACCG AAGCTTCAAA CAACCACTGG CGTGGACTTC CCGAGTTAAC CAATCGCGAG 
GGCGAGTATT GCGCTGATAG CTGTCGCACT CAGGCCTGGA GCGCTTCCGC AATTGTCGAG 
GTCCTCCACG ATTTGGACAA GCTGAAAAAG GATCTGAAGT TTGACGACGA GCAGGTCCTC 
ACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016842157.1
CDS	655..5523
Translation	MVALQNLVCDSTRQLFKKIGYFFHRCLTLFWSYLTNVKSTFLNLQLHLQRAIDDSKITPKKKTMENLGFSADTQNDDQVRVLTLNKGEHQDGTLYKLKKGWVVEFRLGASLLGHNLNIFTNHPLLVGNPFERRTYYQLPWNHESAFIRVSLAGSFHFYLAEASGEDAGKVIASGYLLVDPELTVGVNNQPLPLDCIQCQTVLSKLMGPFSTWEEKLRVSRKSGYNMIHFTPIQAIGDSKSAYSLSDQLKLNPSFNDAPNKETSFDDVEKLITKMREEWNTVSICDIVLNHTANESPFLINNPECSYNCNNCPHLRPSYLLDAILFELTIRVSSGEWEHKGIPPVVETEEHLNAIRHALHTYFLPLAKIHELYTVDINEIVSEFLNIARNQIPQDEPPETHNESIVIIRDPEYRRLKSTIDMQLALKIYNIYRPDCFDEDSRLKRCAEELRNKLQELNDAATNEIQNHLNAAVENSVAGIRYFRVQADGPRQKEISERNPLVPRYFTDYGSPRSFVEREDIMYSNSGCYLMAHNGWVMNSDPLKNFADADSHVYIRRELIAWGDSVKLRYGEKPEDCPALWERMTTYVQQMAKIFDGVRLDNCHSTPIPVAEHMLDAARQVRPDLYVAAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFIQARHRPLAPSIAHALFLDQTHDNPSPIEKRSVFDLLPSAALVSMACCASGSNRGYDELVPHHIHVVDEDRQYTSWTDKEGLAKENAKYVSKKSGIISAKKALNDLHFWLGQNSFSQVFVDQMDVDVVAVTRHSPTTHESVVLVAFTAFKHPDPNASDLRRHVRPLRVEGVVDEIILEASLAHGEKENGIPPFSNPKKYVKNEDYINGYSEYLVDVKEHIQICDSTKVEKVDSGDPKITQLNFINFQPGSIITIRVSLHANIKPALESINDLISTLTSPNNSELRAIVSRMNFADLNKALYRCDQEERDTTANKFGVYDIPGYGPLVYAGLQGVISLLADIRSNNDLGHPLCGNLRQGNWLIDYTWQRLKEDEGTAALGEWLEQACEPFKLIPRYLVPSYFDVIIVNVYSTLLDQCFNLMSSFVKDGTDFTRLLSLVSVQVGGIIKSAQLPDLSPNLDPPKPSIREVDGKSEQECLTMSAGLPHFTVGYMRNWGRDTFIALRGLFLLTGRHAEARFIILGYAATLRHGLIPNLLDRGVNARFNCRDAVWWWLYTIQCYVKEVPNGLKLLSDKVSRLFPTDDSDALPAGEVDETLSDVMQEALTVHFQGLCFRERNAGKRIDEHMTDRGFNNQIGVHPETGFVFGGNEANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIIGLCHSTLNFLAELYKQNLFPYGSVQRKNRDGSTITWSYKQWADKIKESFDTYFYVNEKPTDGELRPDLIHRRGIYKDTHGASQPWADYQLRPNFPVAMVASPNLFKPEHAWLALKKAEEILLGPLGMKTLDPADWAYDGFYNNANDSNDPKVAHGWNYHQGPEWLWPIGYFLRARLHYAPLVGGEEELKRTIASTEAIISRHLTEASNNHWRGLPELTNREGEYCADSCRTQAWSASAIVEVLHDLDKLKKDLKFDDEQVLTN

Target ORF information:

RefSeq Version	XM_016986668.1
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016986668.1

ORF Insert Sequence:

ATGGTTGCTC TGCAGAATCT AGTGTGTGAC TCCACACGAC AATTATTCAA GAAGATAGGG 
TATTTCTTTC ACCGCTGCTT GACCTTATTT TGGTCTTATC TTACCAATGT GAAATCAACA 
TTTCTAAATT TACAGTTACA TCTGCAACGT GCAATCGACG ATTCCAAAAT TACACCCAAA 
AAGAAGACCA TGGAAAATCT CGGCTTCTCT GCGGATACCC AGAACGACGA CCAAGTGCGA 
GTACTGACTC TGAATAAGGG AGAGCACCAG GATGGGACCC TGTACAAGCT TAAAAAAGGA 
TGGGTCGTGG AGTTCAGATT AGGGGCATCG CTTCTGGGCC ACAATCTCAA CATATTCACT 
AATCATCCTC TACTCGTCGG CAACCCGTTC GAGAGGCGCA CGTACTATCA GCTGCCATGG 
AATCACGAGT CAGCTTTCAT ACGCGTCTCG CTGGCCGGCT CTTTTCATTT TTATCTGGCA 
GAAGCGAGTG GAGAGGACGC CGGAAAAGTG ATAGCTTCCG GCTACTTGTT GGTCGATCCG 
GAGCTAACCG TGGGCGTCAA CAACCAGCCG CTGCCGCTAG ACTGCATACA ATGCCAGACG 
GTCCTCTCAA AGCTCATGGG ACCCTTCTCA ACGTGGGAGG AGAAGCTCAG GGTCTCTCGC 
AAGTCAGGCT ACAACATGAT CCACTTCACA CCGATACAGG CGATAGGAGA CTCAAAATCA 
GCTTACAGCC TCAGCGATCA GCTGAAGCTC AACCCCTCGT TCAATGATGC ACCGAACAAA 
GAGACCAGCT TCGACGACGT CGAGAAGTTG ATTACAAAAA TGAGAGAAGA GTGGAACACT 
GTGAGCATTT GCGACATAGT TTTGAATCAC ACGGCGAACG AGAGTCCATT TTTGATAAAC 
AATCCCGAAT GTAGTTACAA CTGCAACAAC TGTCCACATC TCCGTCCGTC TTATCTTTTG 
GATGCCATTT TATTCGAGTT GACAATACGA GTGTCGTCCG GAGAATGGGA GCACAAAGGA 
ATTCCTCCCG TCGTCGAGAC TGAAGAGCAC TTGAACGCAA TCCGTCATGC TCTGCACACT 
TACTTTCTAC CTCTGGCGAA AATTCACGAA CTGTATACTG TAGATATCAA CGAGATCGTG 
TCCGAGTTTT TGAACATTGC TCGCAACCAG ATACCGCAAG ACGAGCCACC CGAAACCCAT 
AACGAAAGCA TCGTTATCAT AAGGGATCCA GAATATCGCC GCTTAAAGTC CACTATAGAT 
ATGCAGCTTG CTTTGAAGAT CTACAATATT TATAGACCCG ATTGTTTTGA CGAGGACTCA 
CGACTGAAAC GCTGCGCGGA AGAACTGAGA AACAAGTTGC AGGAGTTAAA CGATGCCGCA 
ACTAACGAAA TCCAAAATCA TCTCAATGCC GCGGTTGAAA ACTCGGTAGC CGGCATTCGA 
TACTTCAGAG TCCAAGCAGA TGGGCCAAGA CAGAAAGAGA TAAGCGAAAG AAATCCACTT 
GTTCCTAGGT ATTTCACGGA TTACGGAAGT CCTAGATCGT TTGTGGAAAG AGAAGACATC 
ATGTACTCGA ATTCTGGATG TTACTTGATG GCTCACAACG GCTGGGTCAT GAACAGCGAT 
CCCTTGAAAA ACTTTGCCGA TGCTGACTCT CATGTTTACA TAAGAAGGGA ATTGATTGCG 
TGGGGCGATA GTGTCAAATT AAGATATGGC GAAAAACCGG AAGATTGCCC TGCTCTTTGG 
GAACGTATGA CGACATATGT TCAGCAAATG GCTAAAATCT TCGACGGCGT CAGACTCGAC 
AATTGTCACT CGACCCCTAT TCCAGTAGCA GAACACATGT TGGATGCTGC TCGTCAAGTA 
AGACCAGATT TGTACGTTGC CGCTGAACTC TTCACCAACT CCGATCAGAA AGATAATATT 
TTCGTCAATC GTCTTGGAAT CACATCGTTG ATAAGAGAGG CCATGTCAGC GTGGGACAGC 
CATGAGGAAG GCCGTCTGGT ATACCGCTAT GGTGGCGAGC CCGTAGGTGC GTTTATCCAG 
GCTCGACATC GTCCTCTGGC ACCGAGTATC GCGCATGCTT TATTCCTTGA TCAGACGCAT 
GACAACCCGA GCCCCATCGA GAAGCGTAGC GTATTTGACT TATTGCCCAG TGCCGCACTG 
GTCTCCATGG CCTGCTGTGC TAGTGGCAGT AATCGCGGTT ACGACGAGCT CGTGCCTCAT 
CATATTCACG TTGTCGATGA AGACAGACAA TATACTTCTT GGACAGATAA GGAAGGACTG 
GCAAAGGAAA ATGCAAAGTA TGTTAGCAAA AAATCGGGAA TCATTTCTGC TAAAAAGGCT 
CTCAATGATC TCCATTTTTG GCTGGGACAG AACTCCTTTT CTCAAGTTTT TGTCGATCAA 
ATGGATGTTG ATGTGGTAGC GGTTACAAGA CATTCACCGA CCACTCACGA GAGCGTCGTA 
CTCGTGGCTT TTACGGCATT CAAGCACCCG GATCCCAATG CTAGCGATTT GAGAAGACAT 
GTTAGACCTT TGAGGGTAGA AGGCGTAGTA GATGAAATTA TTCTTGAAGC ATCCCTCGCG 
CACGGAGAAA AAGAAAATGG TATCCCTCCG TTCTCTAATC CGAAGAAATA CGTAAAGAAC 
GAAGACTACA TAAACGGTTA CTCTGAGTAC TTGGTGGACG TCAAGGAGCA CATTCAAATC 
TGTGATTCTA CAAAAGTTGA AAAGGTCGAT TCCGGAGATC CTAAAATAAC GCAATTGAAC 
TTTATTAATT TCCAGCCCGG ATCAATAATC ACTATTCGGG TGTCACTCCA CGCAAACATA 
AAGCCAGCTC TTGAATCCAT CAATGACCTT ATCTCGACCC TCACATCTCC GAACAATTCG 
GAATTGCGTG CAATTGTTTC GCGCATGAAC TTTGCGGACC TTAACAAAGC ACTTTATAGG 
TGTGATCAAG AAGAGCGAGA TACGACTGCC AATAAATTTG GAGTTTATGA TATTCCCGGT 
TATGGCCCAC TAGTTTACGC TGGTTTGCAA GGTGTAATTT CATTGCTTGC TGATATCAGA 
TCGAACAACG ATCTTGGTCA TCCACTATGT GGCAACCTTC GTCAAGGCAA TTGGTTGATA 
GATTATACTT GGCAACGTTT GAAGGAAGAT GAAGGTACTG CAGCTCTTGG AGAGTGGCTG 
GAGCAGGCCT GCGAACCATT CAAGCTAATT CCTCGTTATT TAGTGCCCAG TTACTTTGAC 
GTGATCATCG TTAACGTGTA TTCTACTCTC TTAGATCAAT GCTTTAATCT CATGTCTAGC 
TTTGTGAAAG ATGGAACCGA TTTCACCAGA CTGCTGTCAC TAGTATCTGT GCAAGTTGGC 
GGTATAATAA AATCAGCGCA ATTGCCAGAT TTATCACCGA ACCTAGATCC ACCGAAACCT 
AGCATACGAG AGGTGGATGG CAAATCAGAA CAGGAATGTT TGACCATGTC TGCTGGACTA 
CCACACTTTA CAGTAGGATA CATGAGAAAC TGGGGACGAG ATACGTTTAT CGCTTTGAGA 
GGTCTGTTCT TATTAACCGG CAGACATGCG GAGGCTCGAT TCATCATTCT GGGATACGCC 
GCTACGCTTC GTCACGGTTT GATACCAAAT CTCTTGGACA GAGGCGTCAA CGCCAGATTC 
AATTGCCGAG ATGCCGTTTG GTGGTGGTTG TACACTATTC AATGCTATGT CAAAGAAGTC 
CCAAATGGCT TAAAACTTCT GTCGGACAAA GTATCTAGAT TGTTCCCTAC TGACGATTCG 
GACGCTCTTC CAGCCGGAGA AGTGGATGAA ACACTGTCGG ATGTCATGCA GGAAGCACTG 
ACAGTTCACT TTCAAGGTCT GTGCTTCCGT GAGCGCAACG CCGGTAAGCG CATCGACGAG 
CACATGACTG ATCGTGGATT CAACAATCAG ATAGGTGTGC ATCCAGAGAC GGGCTTTGTA 
TTTGGCGGTA ACGAGGCCAA TTGCGGCACT TGGATGGACA AGATGGGCTC CTCGGAAAAG 
GCAGGAAACA AAGGCAAACC TGCCACTCCC AGAGACGGAT CTGCGGTTGA AATTATTGGG 
CTCTGTCACA GCACTCTCAA TTTTCTGGCA GAATTGTACA AACAGAATCT ATTTCCATAT 
GGTAGCGTGC AGCGGAAAAA TCGCGATGGC TCAACGATAA CTTGGAGTTA CAAACAGTGG 
GCGGATAAGA TAAAGGAGAG TTTCGATACT TACTTCTATG TGAACGAGAA ACCTACCGAT 
GGTGAATTAC GACCGGACTT GATTCATCGA CGAGGAATTT ACAAAGATAC TCACGGAGCC 
TCACAACCGT GGGCAGATTA TCAATTGCGA CCTAACTTCC CAGTGGCTAT GGTTGCTTCG 
CCGAACCTAT TCAAGCCCGA GCACGCCTGG CTGGCATTAA AGAAGGCCGA AGAAATCCTC 
CTTGGTCCCC TAGGCATGAA AACCCTAGAT CCCGCTGACT GGGCCTACGA TGGCTTCTAC 
AACAATGCCA ACGACTCGAA TGACCCGAAA GTAGCTCACG GATGGAATTA TCACCAGGGA 
CCAGAGTGGC TATGGCCGAT CGGCTACTTT TTGCGAGCGC GCTTGCACTA CGCGCCCCTC 
GTTGGTGGCG AAGAGGAGTT AAAACGGACT ATCGCCTCGA CCGAAGCTAT CATCTCGAGA 
CATCTGACCG AAGCTTCAAA CAACCACTGG CGTGGACTTC CCGAGTTAAC CAATCGCGAG 
GGCGAGTATT GCGCTGATAG CTGTCGCACT CAGGCCTGGA GCGCTTCCGC AATTGTCGAG 
GTCCTCCACG ATTTGGACAA GCTGAAAAAG GATCTGAAGT TTGACGACGA GCAGGTCCTC 
ACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100117168 ( XM_016986670.1 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_016842159.1 Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X3, mRNA.

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CloneID	ONd18429
Clone ID Related Accession (Same CDS sequence)	XM_016986669.1 , XM_016986668.1 , XM_016986670.1 , XM_016986668.2
Accession Version	XM_016986670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-25
Organism	Nasonia vitripennis(jewel wasp)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001815014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGCTC TGCAGAATCT AGTGTGTGAC TCCACACGAC AATTATTCAA GAAGATAGGG 
TATTTCTTTC ACCGCTGCTT GACCTTATTT TGGTCTTATC TTACCAATGT GAAATCAACA 
TTTCTAAATT TACAGTTACA TCTGCAACGT GCAATCGACG ATTCCAAAAT TACACCCAAA 
AAGAAGACCA TGGAAAATCT CGGCTTCTCT GCGGATACCC AGAACGACGA CCAAGTGCGA 
GTACTGACTC TGAATAAGGG AGAGCACCAG GATGGGACCC TGTACAAGCT TAAAAAAGGA 
TGGGTCGTGG AGTTCAGATT AGGGGCATCG CTTCTGGGCC ACAATCTCAA CATATTCACT 
AATCATCCTC TACTCGTCGG CAACCCGTTC GAGAGGCGCA CGTACTATCA GCTGCCATGG 
AATCACGAGT CAGCTTTCAT ACGCGTCTCG CTGGCCGGCT CTTTTCATTT TTATCTGGCA 
GAAGCGAGTG GAGAGGACGC CGGAAAAGTG ATAGCTTCCG GCTACTTGTT GGTCGATCCG 
GAGCTAACCG TGGGCGTCAA CAACCAGCCG CTGCCGCTAG ACTGCATACA ATGCCAGACG 
GTCCTCTCAA AGCTCATGGG ACCCTTCTCA ACGTGGGAGG AGAAGCTCAG GGTCTCTCGC 
AAGTCAGGCT ACAACATGAT CCACTTCACA CCGATACAGG CGATAGGAGA CTCAAAATCA 
GCTTACAGCC TCAGCGATCA GCTGAAGCTC AACCCCTCGT TCAATGATGC ACCGAACAAA 
GAGACCAGCT TCGACGACGT CGAGAAGTTG ATTACAAAAA TGAGAGAAGA GTGGAACACT 
GTGAGCATTT GCGACATAGT TTTGAATCAC ACGGCGAACG AGAGTCCATT TTTGATAAAC 
AATCCCGAAT GTAGTTACAA CTGCAACAAC TGTCCACATC TCCGTCCGTC TTATCTTTTG 
GATGCCATTT TATTCGAGTT GACAATACGA GTGTCGTCCG GAGAATGGGA GCACAAAGGA 
ATTCCTCCCG TCGTCGAGAC TGAAGAGCAC TTGAACGCAA TCCGTCATGC TCTGCACACT 
TACTTTCTAC CTCTGGCGAA AATTCACGAA CTGTATACTG TAGATATCAA CGAGATCGTG 
TCCGAGTTTT TGAACATTGC TCGCAACCAG ATACCGCAAG ACGAGCCACC CGAAACCCAT 
AACGAAAGCA TCGTTATCAT AAGGGATCCA GAATATCGCC GCTTAAAGTC CACTATAGAT 
ATGCAGCTTG CTTTGAAGAT CTACAATATT TATAGACCCG ATTGTTTTGA CGAGGACTCA 
CGACTGAAAC GCTGCGCGGA AGAACTGAGA AACAAGTTGC AGGAGTTAAA CGATGCCGCA 
ACTAACGAAA TCCAAAATCA TCTCAATGCC GCGGTTGAAA ACTCGGTAGC CGGCATTCGA 
TACTTCAGAG TCCAAGCAGA TGGGCCAAGA CAGAAAGAGA TAAGCGAAAG AAATCCACTT 
GTTCCTAGGT ATTTCACGGA TTACGGAAGT CCTAGATCGT TTGTGGAAAG AGAAGACATC 
ATGTACTCGA ATTCTGGATG TTACTTGATG GCTCACAACG GCTGGGTCAT GAACAGCGAT 
CCCTTGAAAA ACTTTGCCGA TGCTGACTCT CATGTTTACA TAAGAAGGGA ATTGATTGCG 
TGGGGCGATA GTGTCAAATT AAGATATGGC GAAAAACCGG AAGATTGCCC TGCTCTTTGG 
GAACGTATGA CGACATATGT TCAGCAAATG GCTAAAATCT TCGACGGCGT CAGACTCGAC 
AATTGTCACT CGACCCCTAT TCCAGTAGCA GAACACATGT TGGATGCTGC TCGTCAAGTA 
AGACCAGATT TGTACGTTGC CGCTGAACTC TTCACCAACT CCGATCAGAA AGATAATATT 
TTCGTCAATC GTCTTGGAAT CACATCGTTG ATAAGAGAGG CCATGTCAGC GTGGGACAGC 
CATGAGGAAG GCCGTCTGGT ATACCGCTAT GGTGGCGAGC CCGTAGGTGC GTTTATCCAG 
GCTCGACATC GTCCTCTGGC ACCGAGTATC GCGCATGCTT TATTCCTTGA TCAGACGCAT 
GACAACCCGA GCCCCATCGA GAAGCGTAGC GTATTTGACT TATTGCCCAG TGCCGCACTG 
GTCTCCATGG CCTGCTGTGC TAGTGGCAGT AATCGCGGTT ACGACGAGCT CGTGCCTCAT 
CATATTCACG TTGTCGATGA AGACAGACAA TATACTTCTT GGACAGATAA GGAAGGACTG 
GCAAAGGAAA ATGCAAAGTA TGTTAGCAAA AAATCGGGAA TCATTTCTGC TAAAAAGGCT 
CTCAATGATC TCCATTTTTG GCTGGGACAG AACTCCTTTT CTCAAGTTTT TGTCGATCAA 
ATGGATGTTG ATGTGGTAGC GGTTACAAGA CATTCACCGA CCACTCACGA GAGCGTCGTA 
CTCGTGGCTT TTACGGCATT CAAGCACCCG GATCCCAATG CTAGCGATTT GAGAAGACAT 
GTTAGACCTT TGAGGGTAGA AGGCGTAGTA GATGAAATTA TTCTTGAAGC ATCCCTCGCG 
CACGGAGAAA AAGAAAATGG TATCCCTCCG TTCTCTAATC CGAAGAAATA CGTAAAGAAC 
GAAGACTACA TAAACGGTTA CTCTGAGTAC TTGGTGGACG TCAAGGAGCA CATTCAAATC 
TGTGATTCTA CAAAAGTTGA AAAGGTCGAT TCCGGAGATC CTAAAATAAC GCAATTGAAC 
TTTATTAATT TCCAGCCCGG ATCAATAATC ACTATTCGGG TGTCACTCCA CGCAAACATA 
AAGCCAGCTC TTGAATCCAT CAATGACCTT ATCTCGACCC TCACATCTCC GAACAATTCG 
GAATTGCGTG CAATTGTTTC GCGCATGAAC TTTGCGGACC TTAACAAAGC ACTTTATAGG 
TGTGATCAAG AAGAGCGAGA TACGACTGCC AATAAATTTG GAGTTTATGA TATTCCCGGT 
TATGGCCCAC TAGTTTACGC TGGTTTGCAA GGTGTAATTT CATTGCTTGC TGATATCAGA 
TCGAACAACG ATCTTGGTCA TCCACTATGT GGCAACCTTC GTCAAGGCAA TTGGTTGATA 
GATTATACTT GGCAACGTTT GAAGGAAGAT GAAGGTACTG CAGCTCTTGG AGAGTGGCTG 
GAGCAGGCCT GCGAACCATT CAAGCTAATT CCTCGTTATT TAGTGCCCAG TTACTTTGAC 
GTGATCATCG TTAACGTGTA TTCTACTCTC TTAGATCAAT GCTTTAATCT CATGTCTAGC 
TTTGTGAAAG ATGGAACCGA TTTCACCAGA CTGCTGTCAC TAGTATCTGT GCAAGTTGGC 
GGTATAATAA AATCAGCGCA ATTGCCAGAT TTATCACCGA ACCTAGATCC ACCGAAACCT 
AGCATACGAG AGGTGGATGG CAAATCAGAA CAGGAATGTT TGACCATGTC TGCTGGACTA 
CCACACTTTA CAGTAGGATA CATGAGAAAC TGGGGACGAG ATACGTTTAT CGCTTTGAGA 
GGTCTGTTCT TATTAACCGG CAGACATGCG GAGGCTCGAT TCATCATTCT GGGATACGCC 
GCTACGCTTC GTCACGGTTT GATACCAAAT CTCTTGGACA GAGGCGTCAA CGCCAGATTC 
AATTGCCGAG ATGCCGTTTG GTGGTGGTTG TACACTATTC AATGCTATGT CAAAGAAGTC 
CCAAATGGCT TAAAACTTCT GTCGGACAAA GTATCTAGAT TGTTCCCTAC TGACGATTCG 
GACGCTCTTC CAGCCGGAGA AGTGGATGAA ACACTGTCGG ATGTCATGCA GGAAGCACTG 
ACAGTTCACT TTCAAGGTCT GTGCTTCCGT GAGCGCAACG CCGGTAAGCG CATCGACGAG 
CACATGACTG ATCGTGGATT CAACAATCAG ATAGGTGTGC ATCCAGAGAC GGGCTTTGTA 
TTTGGCGGTA ACGAGGCCAA TTGCGGCACT TGGATGGACA AGATGGGCTC CTCGGAAAAG 
GCAGGAAACA AAGGCAAACC TGCCACTCCC AGAGACGGAT CTGCGGTTGA AATTATTGGG 
CTCTGTCACA GCACTCTCAA TTTTCTGGCA GAATTGTACA AACAGAATCT ATTTCCATAT 
GGTAGCGTGC AGCGGAAAAA TCGCGATGGC TCAACGATAA CTTGGAGTTA CAAACAGTGG 
GCGGATAAGA TAAAGGAGAG TTTCGATACT TACTTCTATG TGAACGAGAA ACCTACCGAT 
GGTGAATTAC GACCGGACTT GATTCATCGA CGAGGAATTT ACAAAGATAC TCACGGAGCC 
TCACAACCGT GGGCAGATTA TCAATTGCGA CCTAACTTCC CAGTGGCTAT GGTTGCTTCG 
CCGAACCTAT TCAAGCCCGA GCACGCCTGG CTGGCATTAA AGAAGGCCGA AGAAATCCTC 
CTTGGTCCCC TAGGCATGAA AACCCTAGAT CCCGCTGACT GGGCCTACGA TGGCTTCTAC 
AACAATGCCA ACGACTCGAA TGACCCGAAA GTAGCTCACG GATGGAATTA TCACCAGGGA 
CCAGAGTGGC TATGGCCGAT CGGCTACTTT TTGCGAGCGC GCTTGCACTA CGCGCCCCTC 
GTTGGTGGCG AAGAGGAGTT AAAACGGACT ATCGCCTCGA CCGAAGCTAT CATCTCGAGA 
CATCTGACCG AAGCTTCAAA CAACCACTGG CGTGGACTTC CCGAGTTAAC CAATCGCGAG 
GGCGAGTATT GCGCTGATAG CTGTCGCACT CAGGCCTGGA GCGCTTCCGC AATTGTCGAG 
GTCCTCCACG ATTTGGACAA GCTGAAAAAG GATCTGAAGT TTGACGACGA GCAGGTCCTC 
ACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016842159.1
CDS	334..5202
Translation	MVALQNLVCDSTRQLFKKIGYFFHRCLTLFWSYLTNVKSTFLNLQLHLQRAIDDSKITPKKKTMENLGFSADTQNDDQVRVLTLNKGEHQDGTLYKLKKGWVVEFRLGASLLGHNLNIFTNHPLLVGNPFERRTYYQLPWNHESAFIRVSLAGSFHFYLAEASGEDAGKVIASGYLLVDPELTVGVNNQPLPLDCIQCQTVLSKLMGPFSTWEEKLRVSRKSGYNMIHFTPIQAIGDSKSAYSLSDQLKLNPSFNDAPNKETSFDDVEKLITKMREEWNTVSICDIVLNHTANESPFLINNPECSYNCNNCPHLRPSYLLDAILFELTIRVSSGEWEHKGIPPVVETEEHLNAIRHALHTYFLPLAKIHELYTVDINEIVSEFLNIARNQIPQDEPPETHNESIVIIRDPEYRRLKSTIDMQLALKIYNIYRPDCFDEDSRLKRCAEELRNKLQELNDAATNEIQNHLNAAVENSVAGIRYFRVQADGPRQKEISERNPLVPRYFTDYGSPRSFVEREDIMYSNSGCYLMAHNGWVMNSDPLKNFADADSHVYIRRELIAWGDSVKLRYGEKPEDCPALWERMTTYVQQMAKIFDGVRLDNCHSTPIPVAEHMLDAARQVRPDLYVAAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFIQARHRPLAPSIAHALFLDQTHDNPSPIEKRSVFDLLPSAALVSMACCASGSNRGYDELVPHHIHVVDEDRQYTSWTDKEGLAKENAKYVSKKSGIISAKKALNDLHFWLGQNSFSQVFVDQMDVDVVAVTRHSPTTHESVVLVAFTAFKHPDPNASDLRRHVRPLRVEGVVDEIILEASLAHGEKENGIPPFSNPKKYVKNEDYINGYSEYLVDVKEHIQICDSTKVEKVDSGDPKITQLNFINFQPGSIITIRVSLHANIKPALESINDLISTLTSPNNSELRAIVSRMNFADLNKALYRCDQEERDTTANKFGVYDIPGYGPLVYAGLQGVISLLADIRSNNDLGHPLCGNLRQGNWLIDYTWQRLKEDEGTAALGEWLEQACEPFKLIPRYLVPSYFDVIIVNVYSTLLDQCFNLMSSFVKDGTDFTRLLSLVSVQVGGIIKSAQLPDLSPNLDPPKPSIREVDGKSEQECLTMSAGLPHFTVGYMRNWGRDTFIALRGLFLLTGRHAEARFIILGYAATLRHGLIPNLLDRGVNARFNCRDAVWWWLYTIQCYVKEVPNGLKLLSDKVSRLFPTDDSDALPAGEVDETLSDVMQEALTVHFQGLCFRERNAGKRIDEHMTDRGFNNQIGVHPETGFVFGGNEANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIIGLCHSTLNFLAELYKQNLFPYGSVQRKNRDGSTITWSYKQWADKIKESFDTYFYVNEKPTDGELRPDLIHRRGIYKDTHGASQPWADYQLRPNFPVAMVASPNLFKPEHAWLALKKAEEILLGPLGMKTLDPADWAYDGFYNNANDSNDPKVAHGWNYHQGPEWLWPIGYFLRARLHYAPLVGGEEELKRTIASTEAIISRHLTEASNNHWRGLPELTNREGEYCADSCRTQAWSASAIVEVLHDLDKLKKDLKFDDEQVLTN

Target ORF information:

RefSeq Version	XM_016986670.1
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016986670.1

ORF Insert Sequence:

ATGGTTGCTC TGCAGAATCT AGTGTGTGAC TCCACACGAC AATTATTCAA GAAGATAGGG 
TATTTCTTTC ACCGCTGCTT GACCTTATTT TGGTCTTATC TTACCAATGT GAAATCAACA 
TTTCTAAATT TACAGTTACA TCTGCAACGT GCAATCGACG ATTCCAAAAT TACACCCAAA 
AAGAAGACCA TGGAAAATCT CGGCTTCTCT GCGGATACCC AGAACGACGA CCAAGTGCGA 
GTACTGACTC TGAATAAGGG AGAGCACCAG GATGGGACCC TGTACAAGCT TAAAAAAGGA 
TGGGTCGTGG AGTTCAGATT AGGGGCATCG CTTCTGGGCC ACAATCTCAA CATATTCACT 
AATCATCCTC TACTCGTCGG CAACCCGTTC GAGAGGCGCA CGTACTATCA GCTGCCATGG 
AATCACGAGT CAGCTTTCAT ACGCGTCTCG CTGGCCGGCT CTTTTCATTT TTATCTGGCA 
GAAGCGAGTG GAGAGGACGC CGGAAAAGTG ATAGCTTCCG GCTACTTGTT GGTCGATCCG 
GAGCTAACCG TGGGCGTCAA CAACCAGCCG CTGCCGCTAG ACTGCATACA ATGCCAGACG 
GTCCTCTCAA AGCTCATGGG ACCCTTCTCA ACGTGGGAGG AGAAGCTCAG GGTCTCTCGC 
AAGTCAGGCT ACAACATGAT CCACTTCACA CCGATACAGG CGATAGGAGA CTCAAAATCA 
GCTTACAGCC TCAGCGATCA GCTGAAGCTC AACCCCTCGT TCAATGATGC ACCGAACAAA 
GAGACCAGCT TCGACGACGT CGAGAAGTTG ATTACAAAAA TGAGAGAAGA GTGGAACACT 
GTGAGCATTT GCGACATAGT TTTGAATCAC ACGGCGAACG AGAGTCCATT TTTGATAAAC 
AATCCCGAAT GTAGTTACAA CTGCAACAAC TGTCCACATC TCCGTCCGTC TTATCTTTTG 
GATGCCATTT TATTCGAGTT GACAATACGA GTGTCGTCCG GAGAATGGGA GCACAAAGGA 
ATTCCTCCCG TCGTCGAGAC TGAAGAGCAC TTGAACGCAA TCCGTCATGC TCTGCACACT 
TACTTTCTAC CTCTGGCGAA AATTCACGAA CTGTATACTG TAGATATCAA CGAGATCGTG 
TCCGAGTTTT TGAACATTGC TCGCAACCAG ATACCGCAAG ACGAGCCACC CGAAACCCAT 
AACGAAAGCA TCGTTATCAT AAGGGATCCA GAATATCGCC GCTTAAAGTC CACTATAGAT 
ATGCAGCTTG CTTTGAAGAT CTACAATATT TATAGACCCG ATTGTTTTGA CGAGGACTCA 
CGACTGAAAC GCTGCGCGGA AGAACTGAGA AACAAGTTGC AGGAGTTAAA CGATGCCGCA 
ACTAACGAAA TCCAAAATCA TCTCAATGCC GCGGTTGAAA ACTCGGTAGC CGGCATTCGA 
TACTTCAGAG TCCAAGCAGA TGGGCCAAGA CAGAAAGAGA TAAGCGAAAG AAATCCACTT 
GTTCCTAGGT ATTTCACGGA TTACGGAAGT CCTAGATCGT TTGTGGAAAG AGAAGACATC 
ATGTACTCGA ATTCTGGATG TTACTTGATG GCTCACAACG GCTGGGTCAT GAACAGCGAT 
CCCTTGAAAA ACTTTGCCGA TGCTGACTCT CATGTTTACA TAAGAAGGGA ATTGATTGCG 
TGGGGCGATA GTGTCAAATT AAGATATGGC GAAAAACCGG AAGATTGCCC TGCTCTTTGG 
GAACGTATGA CGACATATGT TCAGCAAATG GCTAAAATCT TCGACGGCGT CAGACTCGAC 
AATTGTCACT CGACCCCTAT TCCAGTAGCA GAACACATGT TGGATGCTGC TCGTCAAGTA 
AGACCAGATT TGTACGTTGC CGCTGAACTC TTCACCAACT CCGATCAGAA AGATAATATT 
TTCGTCAATC GTCTTGGAAT CACATCGTTG ATAAGAGAGG CCATGTCAGC GTGGGACAGC 
CATGAGGAAG GCCGTCTGGT ATACCGCTAT GGTGGCGAGC CCGTAGGTGC GTTTATCCAG 
GCTCGACATC GTCCTCTGGC ACCGAGTATC GCGCATGCTT TATTCCTTGA TCAGACGCAT 
GACAACCCGA GCCCCATCGA GAAGCGTAGC GTATTTGACT TATTGCCCAG TGCCGCACTG 
GTCTCCATGG CCTGCTGTGC TAGTGGCAGT AATCGCGGTT ACGACGAGCT CGTGCCTCAT 
CATATTCACG TTGTCGATGA AGACAGACAA TATACTTCTT GGACAGATAA GGAAGGACTG 
GCAAAGGAAA ATGCAAAGTA TGTTAGCAAA AAATCGGGAA TCATTTCTGC TAAAAAGGCT 
CTCAATGATC TCCATTTTTG GCTGGGACAG AACTCCTTTT CTCAAGTTTT TGTCGATCAA 
ATGGATGTTG ATGTGGTAGC GGTTACAAGA CATTCACCGA CCACTCACGA GAGCGTCGTA 
CTCGTGGCTT TTACGGCATT CAAGCACCCG GATCCCAATG CTAGCGATTT GAGAAGACAT 
GTTAGACCTT TGAGGGTAGA AGGCGTAGTA GATGAAATTA TTCTTGAAGC ATCCCTCGCG 
CACGGAGAAA AAGAAAATGG TATCCCTCCG TTCTCTAATC CGAAGAAATA CGTAAAGAAC 
GAAGACTACA TAAACGGTTA CTCTGAGTAC TTGGTGGACG TCAAGGAGCA CATTCAAATC 
TGTGATTCTA CAAAAGTTGA AAAGGTCGAT TCCGGAGATC CTAAAATAAC GCAATTGAAC 
TTTATTAATT TCCAGCCCGG ATCAATAATC ACTATTCGGG TGTCACTCCA CGCAAACATA 
AAGCCAGCTC TTGAATCCAT CAATGACCTT ATCTCGACCC TCACATCTCC GAACAATTCG 
GAATTGCGTG CAATTGTTTC GCGCATGAAC TTTGCGGACC TTAACAAAGC ACTTTATAGG 
TGTGATCAAG AAGAGCGAGA TACGACTGCC AATAAATTTG GAGTTTATGA TATTCCCGGT 
TATGGCCCAC TAGTTTACGC TGGTTTGCAA GGTGTAATTT CATTGCTTGC TGATATCAGA 
TCGAACAACG ATCTTGGTCA TCCACTATGT GGCAACCTTC GTCAAGGCAA TTGGTTGATA 
GATTATACTT GGCAACGTTT GAAGGAAGAT GAAGGTACTG CAGCTCTTGG AGAGTGGCTG 
GAGCAGGCCT GCGAACCATT CAAGCTAATT CCTCGTTATT TAGTGCCCAG TTACTTTGAC 
GTGATCATCG TTAACGTGTA TTCTACTCTC TTAGATCAAT GCTTTAATCT CATGTCTAGC 
TTTGTGAAAG ATGGAACCGA TTTCACCAGA CTGCTGTCAC TAGTATCTGT GCAAGTTGGC 
GGTATAATAA AATCAGCGCA ATTGCCAGAT TTATCACCGA ACCTAGATCC ACCGAAACCT 
AGCATACGAG AGGTGGATGG CAAATCAGAA CAGGAATGTT TGACCATGTC TGCTGGACTA 
CCACACTTTA CAGTAGGATA CATGAGAAAC TGGGGACGAG ATACGTTTAT CGCTTTGAGA 
GGTCTGTTCT TATTAACCGG CAGACATGCG GAGGCTCGAT TCATCATTCT GGGATACGCC 
GCTACGCTTC GTCACGGTTT GATACCAAAT CTCTTGGACA GAGGCGTCAA CGCCAGATTC 
AATTGCCGAG ATGCCGTTTG GTGGTGGTTG TACACTATTC AATGCTATGT CAAAGAAGTC 
CCAAATGGCT TAAAACTTCT GTCGGACAAA GTATCTAGAT TGTTCCCTAC TGACGATTCG 
GACGCTCTTC CAGCCGGAGA AGTGGATGAA ACACTGTCGG ATGTCATGCA GGAAGCACTG 
ACAGTTCACT TTCAAGGTCT GTGCTTCCGT GAGCGCAACG CCGGTAAGCG CATCGACGAG 
CACATGACTG ATCGTGGATT CAACAATCAG ATAGGTGTGC ATCCAGAGAC GGGCTTTGTA 
TTTGGCGGTA ACGAGGCCAA TTGCGGCACT TGGATGGACA AGATGGGCTC CTCGGAAAAG 
GCAGGAAACA AAGGCAAACC TGCCACTCCC AGAGACGGAT CTGCGGTTGA AATTATTGGG 
CTCTGTCACA GCACTCTCAA TTTTCTGGCA GAATTGTACA AACAGAATCT ATTTCCATAT 
GGTAGCGTGC AGCGGAAAAA TCGCGATGGC TCAACGATAA CTTGGAGTTA CAAACAGTGG 
GCGGATAAGA TAAAGGAGAG TTTCGATACT TACTTCTATG TGAACGAGAA ACCTACCGAT 
GGTGAATTAC GACCGGACTT GATTCATCGA CGAGGAATTT ACAAAGATAC TCACGGAGCC 
TCACAACCGT GGGCAGATTA TCAATTGCGA CCTAACTTCC CAGTGGCTAT GGTTGCTTCG 
CCGAACCTAT TCAAGCCCGA GCACGCCTGG CTGGCATTAA AGAAGGCCGA AGAAATCCTC 
CTTGGTCCCC TAGGCATGAA AACCCTAGAT CCCGCTGACT GGGCCTACGA TGGCTTCTAC 
AACAATGCCA ACGACTCGAA TGACCCGAAA GTAGCTCACG GATGGAATTA TCACCAGGGA 
CCAGAGTGGC TATGGCCGAT CGGCTACTTT TTGCGAGCGC GCTTGCACTA CGCGCCCCTC 
GTTGGTGGCG AAGAGGAGTT AAAACGGACT ATCGCCTCGA CCGAAGCTAT CATCTCGAGA 
CATCTGACCG AAGCTTCAAA CAACCACTGG CGTGGACTTC CCGAGTTAAC CAATCGCGAG 
GGCGAGTATT GCGCTGATAG CTGTCGCACT CAGGCCTGGA GCGCTTCCGC AATTGTCGAG 
GTCCTCCACG ATTTGGACAA GCTGAAAAAG GATCTGAAGT TTGACGACGA GCAGGTCCTC 
ACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100117168 ( XM_016986668.2 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_016842157.1 Nasonia vitripennis glycogen debranching enzyme (LOC100117168), mRNA.

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CloneID	ONd18429
Clone ID Related Accession (Same CDS sequence)	XM_016986669.1 , XM_016986668.1 , XM_016986670.1 , XM_016986668.2
Accession Version	XM_016986668.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Nasonia vitripennis(jewel wasp)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022279592.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 24, 2019 this sequence version replaced XM_016986668.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nasonia vitripennis Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGCTC TGCAGAATCT AGTGTGTGAC TCCACACGAC AATTATTCAA GAAGATAGGG 
TATTTCTTTC ACCGCTGCTT GACCTTATTT TGGTCTTATC TTACCAATGT GAAATCAACA 
TTTCTAAATT TACAGTTACA TCTGCAACGT GCAATCGACG ATTCCAAAAT TACACCCAAA 
AAGAAGACCA TGGAAAATCT CGGCTTCTCT GCGGATACCC AGAACGACGA CCAAGTGCGA 
GTACTGACTC TGAATAAGGG AGAGCACCAG GATGGGACCC TGTACAAGCT TAAAAAAGGA 
TGGGTCGTGG AGTTCAGATT AGGGGCATCG CTTCTGGGCC ACAATCTCAA CATATTCACT 
AATCATCCTC TACTCGTCGG CAACCCGTTC GAGAGGCGCA CGTACTATCA GCTGCCATGG 
AATCACGAGT CAGCTTTCAT ACGCGTCTCG CTGGCCGGCT CTTTTCATTT TTATCTGGCA 
GAAGCGAGTG GAGAGGACGC CGGAAAAGTG ATAGCTTCCG GCTACTTGTT GGTCGATCCG 
GAGCTAACCG TGGGCGTCAA CAACCAGCCG CTGCCGCTAG ACTGCATACA ATGCCAGACG 
GTCCTCTCAA AGCTCATGGG ACCCTTCTCA ACGTGGGAGG AGAAGCTCAG GGTCTCTCGC 
AAGTCAGGCT ACAACATGAT CCACTTCACA CCGATACAGG CGATAGGAGA CTCAAAATCA 
GCTTACAGCC TCAGCGATCA GCTGAAGCTC AACCCCTCGT TCAATGATGC ACCGAACAAA 
GAGACCAGCT TCGACGACGT CGAGAAGTTG ATTACAAAAA TGAGAGAAGA GTGGAACACT 
GTGAGCATTT GCGACATAGT TTTGAATCAC ACGGCGAACG AGAGTCCATT TTTGATAAAC 
AATCCCGAAT GTAGTTACAA CTGCAACAAC TGTCCACATC TCCGTCCGTC TTATCTTTTG 
GATGCCATTT TATTCGAGTT GACAATACGA GTGTCGTCCG GAGAATGGGA GCACAAAGGA 
ATTCCTCCCG TCGTCGAGAC TGAAGAGCAC TTGAACGCAA TCCGTCATGC TCTGCACACT 
TACTTTCTAC CTCTGGCGAA AATTCACGAA CTGTATACTG TAGATATCAA CGAGATCGTG 
TCCGAGTTTT TGAACATTGC TCGCAACCAG ATACCGCAAG ACGAGCCACC CGAAACCCAT 
AACGAAAGCA TCGTTATCAT AAGGGATCCA GAATATCGCC GCTTAAAGTC CACTATAGAT 
ATGCAGCTTG CTTTGAAGAT CTACAATATT TATAGACCCG ATTGTTTTGA CGAGGACTCA 
CGACTGAAAC GCTGCGCGGA AGAACTGAGA AACAAGTTGC AGGAGTTAAA CGATGCCGCA 
ACTAACGAAA TCCAAAATCA TCTCAATGCC GCGGTTGAAA ACTCGGTAGC CGGCATTCGA 
TACTTCAGAG TCCAAGCAGA TGGGCCAAGA CAGAAAGAGA TAAGCGAAAG AAATCCACTT 
GTTCCTAGGT ATTTCACGGA TTACGGAAGT CCTAGATCGT TTGTGGAAAG AGAAGACATC 
ATGTACTCGA ATTCTGGATG TTACTTGATG GCTCACAACG GCTGGGTCAT GAACAGCGAT 
CCCTTGAAAA ACTTTGCCGA TGCTGACTCT CATGTTTACA TAAGAAGGGA ATTGATTGCG 
TGGGGCGATA GTGTCAAATT AAGATATGGC GAAAAACCGG AAGATTGCCC TGCTCTTTGG 
GAACGTATGA CGACATATGT TCAGCAAATG GCTAAAATCT TCGACGGCGT CAGACTCGAC 
AATTGTCACT CGACCCCTAT TCCAGTAGCA GAACACATGT TGGATGCTGC TCGTCAAGTA 
AGACCAGATT TGTACGTTGC CGCTGAACTC TTCACCAACT CCGATCAGAA AGATAATATT 
TTCGTCAATC GTCTTGGAAT CACATCGTTG ATAAGAGAGG CCATGTCAGC GTGGGACAGC 
CATGAGGAAG GCCGTCTGGT ATACCGCTAT GGTGGCGAGC CCGTAGGTGC GTTTATCCAG 
GCTCGACATC GTCCTCTGGC ACCGAGTATC GCGCATGCTT TATTCCTTGA TCAGACGCAT 
GACAACCCGA GCCCCATCGA GAAGCGTAGC GTATTTGACT TATTGCCCAG TGCCGCACTG 
GTCTCCATGG CCTGCTGTGC TAGTGGCAGT AATCGCGGTT ACGACGAGCT CGTGCCTCAT 
CATATTCACG TTGTCGATGA AGACAGACAA TATACTTCTT GGACAGATAA GGAAGGACTG 
GCAAAGGAAA ATGCAAAGTA TGTTAGCAAA AAATCGGGAA TCATTTCTGC TAAAAAGGCT 
CTCAATGATC TCCATTTTTG GCTGGGACAG AACTCCTTTT CTCAAGTTTT TGTCGATCAA 
ATGGATGTTG ATGTGGTAGC GGTTACAAGA CATTCACCGA CCACTCACGA GAGCGTCGTA 
CTCGTGGCTT TTACGGCATT CAAGCACCCG GATCCCAATG CTAGCGATTT GAGAAGACAT 
GTTAGACCTT TGAGGGTAGA AGGCGTAGTA GATGAAATTA TTCTTGAAGC ATCCCTCGCG 
CACGGAGAAA AAGAAAATGG TATCCCTCCG TTCTCTAATC CGAAGAAATA CGTAAAGAAC 
GAAGACTACA TAAACGGTTA CTCTGAGTAC TTGGTGGACG TCAAGGAGCA CATTCAAATC 
TGTGATTCTA CAAAAGTTGA AAAGGTCGAT TCCGGAGATC CTAAAATAAC GCAATTGAAC 
TTTATTAATT TCCAGCCCGG ATCAATAATC ACTATTCGGG TGTCACTCCA CGCAAACATA 
AAGCCAGCTC TTGAATCCAT CAATGACCTT ATCTCGACCC TCACATCTCC GAACAATTCG 
GAATTGCGTG CAATTGTTTC GCGCATGAAC TTTGCGGACC TTAACAAAGC ACTTTATAGG 
TGTGATCAAG AAGAGCGAGA TACGACTGCC AATAAATTTG GAGTTTATGA TATTCCCGGT 
TATGGCCCAC TAGTTTACGC TGGTTTGCAA GGTGTAATTT CATTGCTTGC TGATATCAGA 
TCGAACAACG ATCTTGGTCA TCCACTATGT GGCAACCTTC GTCAAGGCAA TTGGTTGATA 
GATTATACTT GGCAACGTTT GAAGGAAGAT GAAGGTACTG CAGCTCTTGG AGAGTGGCTG 
GAGCAGGCCT GCGAACCATT CAAGCTAATT CCTCGTTATT TAGTGCCCAG TTACTTTGAC 
GTGATCATCG TTAACGTGTA TTCTACTCTC TTAGATCAAT GCTTTAATCT CATGTCTAGC 
TTTGTGAAAG ATGGAACCGA TTTCACCAGA CTGCTGTCAC TAGTATCTGT GCAAGTTGGC 
GGTATAATAA AATCAGCGCA ATTGCCAGAT TTATCACCGA ACCTAGATCC ACCGAAACCT 
AGCATACGAG AGGTGGATGG CAAATCAGAA CAGGAATGTT TGACCATGTC TGCTGGACTA 
CCACACTTTA CAGTAGGATA CATGAGAAAC TGGGGACGAG ATACGTTTAT CGCTTTGAGA 
GGTCTGTTCT TATTAACCGG CAGACATGCG GAGGCTCGAT TCATCATTCT GGGATACGCC 
GCTACGCTTC GTCACGGTTT GATACCAAAT CTCTTGGACA GAGGCGTCAA CGCCAGATTC 
AATTGCCGAG ATGCCGTTTG GTGGTGGTTG TACACTATTC AATGCTATGT CAAAGAAGTC 
CCAAATGGCT TAAAACTTCT GTCGGACAAA GTATCTAGAT TGTTCCCTAC TGACGATTCG 
GACGCTCTTC CAGCCGGAGA AGTGGATGAA ACACTGTCGG ATGTCATGCA GGAAGCACTG 
ACAGTTCACT TTCAAGGTCT GTGCTTCCGT GAGCGCAACG CCGGTAAGCG CATCGACGAG 
CACATGACTG ATCGTGGATT CAACAATCAG ATAGGTGTGC ATCCAGAGAC GGGCTTTGTA 
TTTGGCGGTA ACGAGGCCAA TTGCGGCACT TGGATGGACA AGATGGGCTC CTCGGAAAAG 
GCAGGAAACA AAGGCAAACC TGCCACTCCC AGAGACGGAT CTGCGGTTGA AATTATTGGG 
CTCTGTCACA GCACTCTCAA TTTTCTGGCA GAATTGTACA AACAGAATCT ATTTCCATAT 
GGTAGCGTGC AGCGGAAAAA TCGCGATGGC TCAACGATAA CTTGGAGTTA CAAACAGTGG 
GCGGATAAGA TAAAGGAGAG TTTCGATACT TACTTCTATG TGAACGAGAA ACCTACCGAT 
GGTGAATTAC GACCGGACTT GATTCATCGA CGAGGAATTT ACAAAGATAC TCACGGAGCC 
TCACAACCGT GGGCAGATTA TCAATTGCGA CCTAACTTCC CAGTGGCTAT GGTTGCTTCG 
CCGAACCTAT TCAAGCCCGA GCACGCCTGG CTGGCATTAA AGAAGGCCGA AGAAATCCTC 
CTTGGTCCCC TAGGCATGAA AACCCTAGAT CCCGCTGACT GGGCCTACGA TGGCTTCTAC 
AACAATGCCA ACGACTCGAA TGACCCGAAA GTAGCTCACG GATGGAATTA TCACCAGGGA 
CCAGAGTGGC TATGGCCGAT CGGCTACTTT TTGCGAGCGC GCTTGCACTA CGCGCCCCTC 
GTTGGTGGCG AAGAGGAGTT AAAACGGACT ATCGCCTCGA CCGAAGCTAT CATCTCGAGA 
CATCTGACCG AAGCTTCAAA CAACCACTGG CGTGGACTTC CCGAGTTAAC CAATCGCGAG 
GGCGAGTATT GCGCTGATAG CTGTCGCACT CAGGCCTGGA GCGCTTCCGC AATTGTCGAG 
GTCCTCCACG ATTTGGACAA GCTGAAAAAG GATCTGAAGT TTGACGACGA GCAGGTCCTC 
ACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016842157.1
CDS	630..5498
Translation	MVALQNLVCDSTRQLFKKIGYFFHRCLTLFWSYLTNVKSTFLNLQLHLQRAIDDSKITPKKKTMENLGFSADTQNDDQVRVLTLNKGEHQDGTLYKLKKGWVVEFRLGASLLGHNLNIFTNHPLLVGNPFERRTYYQLPWNHESAFIRVSLAGSFHFYLAEASGEDAGKVIASGYLLVDPELTVGVNNQPLPLDCIQCQTVLSKLMGPFSTWEEKLRVSRKSGYNMIHFTPIQAIGDSKSAYSLSDQLKLNPSFNDAPNKETSFDDVEKLITKMREEWNTVSICDIVLNHTANESPFLINNPECSYNCNNCPHLRPSYLLDAILFELTIRVSSGEWEHKGIPPVVETEEHLNAIRHALHTYFLPLAKIHELYTVDINEIVSEFLNIARNQIPQDEPPETHNESIVIIRDPEYRRLKSTIDMQLALKIYNIYRPDCFDEDSRLKRCAEELRNKLQELNDAATNEIQNHLNAAVENSVAGIRYFRVQADGPRQKEISERNPLVPRYFTDYGSPRSFVEREDIMYSNSGCYLMAHNGWVMNSDPLKNFADADSHVYIRRELIAWGDSVKLRYGEKPEDCPALWERMTTYVQQMAKIFDGVRLDNCHSTPIPVAEHMLDAARQVRPDLYVAAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFIQARHRPLAPSIAHALFLDQTHDNPSPIEKRSVFDLLPSAALVSMACCASGSNRGYDELVPHHIHVVDEDRQYTSWTDKEGLAKENAKYVSKKSGIISAKKALNDLHFWLGQNSFSQVFVDQMDVDVVAVTRHSPTTHESVVLVAFTAFKHPDPNASDLRRHVRPLRVEGVVDEIILEASLAHGEKENGIPPFSNPKKYVKNEDYINGYSEYLVDVKEHIQICDSTKVEKVDSGDPKITQLNFINFQPGSIITIRVSLHANIKPALESINDLISTLTSPNNSELRAIVSRMNFADLNKALYRCDQEERDTTANKFGVYDIPGYGPLVYAGLQGVISLLADIRSNNDLGHPLCGNLRQGNWLIDYTWQRLKEDEGTAALGEWLEQACEPFKLIPRYLVPSYFDVIIVNVYSTLLDQCFNLMSSFVKDGTDFTRLLSLVSVQVGGIIKSAQLPDLSPNLDPPKPSIREVDGKSEQECLTMSAGLPHFTVGYMRNWGRDTFIALRGLFLLTGRHAEARFIILGYAATLRHGLIPNLLDRGVNARFNCRDAVWWWLYTIQCYVKEVPNGLKLLSDKVSRLFPTDDSDALPAGEVDETLSDVMQEALTVHFQGLCFRERNAGKRIDEHMTDRGFNNQIGVHPETGFVFGGNEANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIIGLCHSTLNFLAELYKQNLFPYGSVQRKNRDGSTITWSYKQWADKIKESFDTYFYVNEKPTDGELRPDLIHRRGIYKDTHGASQPWADYQLRPNFPVAMVASPNLFKPEHAWLALKKAEEILLGPLGMKTLDPADWAYDGFYNNANDSNDPKVAHGWNYHQGPEWLWPIGYFLRARLHYAPLVGGEEELKRTIASTEAIISRHLTEASNNHWRGLPELTNREGEYCADSCRTQAWSASAIVEVLHDLDKLKKDLKFDDEQVLTN

Target ORF information:

RefSeq Version	XM_016986668.2
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016986668.2

ORF Insert Sequence:

ATGGTTGCTC TGCAGAATCT AGTGTGTGAC TCCACACGAC AATTATTCAA GAAGATAGGG 
TATTTCTTTC ACCGCTGCTT GACCTTATTT TGGTCTTATC TTACCAATGT GAAATCAACA 
TTTCTAAATT TACAGTTACA TCTGCAACGT GCAATCGACG ATTCCAAAAT TACACCCAAA 
AAGAAGACCA TGGAAAATCT CGGCTTCTCT GCGGATACCC AGAACGACGA CCAAGTGCGA 
GTACTGACTC TGAATAAGGG AGAGCACCAG GATGGGACCC TGTACAAGCT TAAAAAAGGA 
TGGGTCGTGG AGTTCAGATT AGGGGCATCG CTTCTGGGCC ACAATCTCAA CATATTCACT 
AATCATCCTC TACTCGTCGG CAACCCGTTC GAGAGGCGCA CGTACTATCA GCTGCCATGG 
AATCACGAGT CAGCTTTCAT ACGCGTCTCG CTGGCCGGCT CTTTTCATTT TTATCTGGCA 
GAAGCGAGTG GAGAGGACGC CGGAAAAGTG ATAGCTTCCG GCTACTTGTT GGTCGATCCG 
GAGCTAACCG TGGGCGTCAA CAACCAGCCG CTGCCGCTAG ACTGCATACA ATGCCAGACG 
GTCCTCTCAA AGCTCATGGG ACCCTTCTCA ACGTGGGAGG AGAAGCTCAG GGTCTCTCGC 
AAGTCAGGCT ACAACATGAT CCACTTCACA CCGATACAGG CGATAGGAGA CTCAAAATCA 
GCTTACAGCC TCAGCGATCA GCTGAAGCTC AACCCCTCGT TCAATGATGC ACCGAACAAA 
GAGACCAGCT TCGACGACGT CGAGAAGTTG ATTACAAAAA TGAGAGAAGA GTGGAACACT 
GTGAGCATTT GCGACATAGT TTTGAATCAC ACGGCGAACG AGAGTCCATT TTTGATAAAC 
AATCCCGAAT GTAGTTACAA CTGCAACAAC TGTCCACATC TCCGTCCGTC TTATCTTTTG 
GATGCCATTT TATTCGAGTT GACAATACGA GTGTCGTCCG GAGAATGGGA GCACAAAGGA 
ATTCCTCCCG TCGTCGAGAC TGAAGAGCAC TTGAACGCAA TCCGTCATGC TCTGCACACT 
TACTTTCTAC CTCTGGCGAA AATTCACGAA CTGTATACTG TAGATATCAA CGAGATCGTG 
TCCGAGTTTT TGAACATTGC TCGCAACCAG ATACCGCAAG ACGAGCCACC CGAAACCCAT 
AACGAAAGCA TCGTTATCAT AAGGGATCCA GAATATCGCC GCTTAAAGTC CACTATAGAT 
ATGCAGCTTG CTTTGAAGAT CTACAATATT TATAGACCCG ATTGTTTTGA CGAGGACTCA 
CGACTGAAAC GCTGCGCGGA AGAACTGAGA AACAAGTTGC AGGAGTTAAA CGATGCCGCA 
ACTAACGAAA TCCAAAATCA TCTCAATGCC GCGGTTGAAA ACTCGGTAGC CGGCATTCGA 
TACTTCAGAG TCCAAGCAGA TGGGCCAAGA CAGAAAGAGA TAAGCGAAAG AAATCCACTT 
GTTCCTAGGT ATTTCACGGA TTACGGAAGT CCTAGATCGT TTGTGGAAAG AGAAGACATC 
ATGTACTCGA ATTCTGGATG TTACTTGATG GCTCACAACG GCTGGGTCAT GAACAGCGAT 
CCCTTGAAAA ACTTTGCCGA TGCTGACTCT CATGTTTACA TAAGAAGGGA ATTGATTGCG 
TGGGGCGATA GTGTCAAATT AAGATATGGC GAAAAACCGG AAGATTGCCC TGCTCTTTGG 
GAACGTATGA CGACATATGT TCAGCAAATG GCTAAAATCT TCGACGGCGT CAGACTCGAC 
AATTGTCACT CGACCCCTAT TCCAGTAGCA GAACACATGT TGGATGCTGC TCGTCAAGTA 
AGACCAGATT TGTACGTTGC CGCTGAACTC TTCACCAACT CCGATCAGAA AGATAATATT 
TTCGTCAATC GTCTTGGAAT CACATCGTTG ATAAGAGAGG CCATGTCAGC GTGGGACAGC 
CATGAGGAAG GCCGTCTGGT ATACCGCTAT GGTGGCGAGC CCGTAGGTGC GTTTATCCAG 
GCTCGACATC GTCCTCTGGC ACCGAGTATC GCGCATGCTT TATTCCTTGA TCAGACGCAT 
GACAACCCGA GCCCCATCGA GAAGCGTAGC GTATTTGACT TATTGCCCAG TGCCGCACTG 
GTCTCCATGG CCTGCTGTGC TAGTGGCAGT AATCGCGGTT ACGACGAGCT CGTGCCTCAT 
CATATTCACG TTGTCGATGA AGACAGACAA TATACTTCTT GGACAGATAA GGAAGGACTG 
GCAAAGGAAA ATGCAAAGTA TGTTAGCAAA AAATCGGGAA TCATTTCTGC TAAAAAGGCT 
CTCAATGATC TCCATTTTTG GCTGGGACAG AACTCCTTTT CTCAAGTTTT TGTCGATCAA 
ATGGATGTTG ATGTGGTAGC GGTTACAAGA CATTCACCGA CCACTCACGA GAGCGTCGTA 
CTCGTGGCTT TTACGGCATT CAAGCACCCG GATCCCAATG CTAGCGATTT GAGAAGACAT 
GTTAGACCTT TGAGGGTAGA AGGCGTAGTA GATGAAATTA TTCTTGAAGC ATCCCTCGCG 
CACGGAGAAA AAGAAAATGG TATCCCTCCG TTCTCTAATC CGAAGAAATA CGTAAAGAAC 
GAAGACTACA TAAACGGTTA CTCTGAGTAC TTGGTGGACG TCAAGGAGCA CATTCAAATC 
TGTGATTCTA CAAAAGTTGA AAAGGTCGAT TCCGGAGATC CTAAAATAAC GCAATTGAAC 
TTTATTAATT TCCAGCCCGG ATCAATAATC ACTATTCGGG TGTCACTCCA CGCAAACATA 
AAGCCAGCTC TTGAATCCAT CAATGACCTT ATCTCGACCC TCACATCTCC GAACAATTCG 
GAATTGCGTG CAATTGTTTC GCGCATGAAC TTTGCGGACC TTAACAAAGC ACTTTATAGG 
TGTGATCAAG AAGAGCGAGA TACGACTGCC AATAAATTTG GAGTTTATGA TATTCCCGGT 
TATGGCCCAC TAGTTTACGC TGGTTTGCAA GGTGTAATTT CATTGCTTGC TGATATCAGA 
TCGAACAACG ATCTTGGTCA TCCACTATGT GGCAACCTTC GTCAAGGCAA TTGGTTGATA 
GATTATACTT GGCAACGTTT GAAGGAAGAT GAAGGTACTG CAGCTCTTGG AGAGTGGCTG 
GAGCAGGCCT GCGAACCATT CAAGCTAATT CCTCGTTATT TAGTGCCCAG TTACTTTGAC 
GTGATCATCG TTAACGTGTA TTCTACTCTC TTAGATCAAT GCTTTAATCT CATGTCTAGC 
TTTGTGAAAG ATGGAACCGA TTTCACCAGA CTGCTGTCAC TAGTATCTGT GCAAGTTGGC 
GGTATAATAA AATCAGCGCA ATTGCCAGAT TTATCACCGA ACCTAGATCC ACCGAAACCT 
AGCATACGAG AGGTGGATGG CAAATCAGAA CAGGAATGTT TGACCATGTC TGCTGGACTA 
CCACACTTTA CAGTAGGATA CATGAGAAAC TGGGGACGAG ATACGTTTAT CGCTTTGAGA 
GGTCTGTTCT TATTAACCGG CAGACATGCG GAGGCTCGAT TCATCATTCT GGGATACGCC 
GCTACGCTTC GTCACGGTTT GATACCAAAT CTCTTGGACA GAGGCGTCAA CGCCAGATTC 
AATTGCCGAG ATGCCGTTTG GTGGTGGTTG TACACTATTC AATGCTATGT CAAAGAAGTC 
CCAAATGGCT TAAAACTTCT GTCGGACAAA GTATCTAGAT TGTTCCCTAC TGACGATTCG 
GACGCTCTTC CAGCCGGAGA AGTGGATGAA ACACTGTCGG ATGTCATGCA GGAAGCACTG 
ACAGTTCACT TTCAAGGTCT GTGCTTCCGT GAGCGCAACG CCGGTAAGCG CATCGACGAG 
CACATGACTG ATCGTGGATT CAACAATCAG ATAGGTGTGC ATCCAGAGAC GGGCTTTGTA 
TTTGGCGGTA ACGAGGCCAA TTGCGGCACT TGGATGGACA AGATGGGCTC CTCGGAAAAG 
GCAGGAAACA AAGGCAAACC TGCCACTCCC AGAGACGGAT CTGCGGTTGA AATTATTGGG 
CTCTGTCACA GCACTCTCAA TTTTCTGGCA GAATTGTACA AACAGAATCT ATTTCCATAT 
GGTAGCGTGC AGCGGAAAAA TCGCGATGGC TCAACGATAA CTTGGAGTTA CAAACAGTGG 
GCGGATAAGA TAAAGGAGAG TTTCGATACT TACTTCTATG TGAACGAGAA ACCTACCGAT 
GGTGAATTAC GACCGGACTT GATTCATCGA CGAGGAATTT ACAAAGATAC TCACGGAGCC 
TCACAACCGT GGGCAGATTA TCAATTGCGA CCTAACTTCC CAGTGGCTAT GGTTGCTTCG 
CCGAACCTAT TCAAGCCCGA GCACGCCTGG CTGGCATTAA AGAAGGCCGA AGAAATCCTC 
CTTGGTCCCC TAGGCATGAA AACCCTAGAT CCCGCTGACT GGGCCTACGA TGGCTTCTAC 
AACAATGCCA ACGACTCGAA TGACCCGAAA GTAGCTCACG GATGGAATTA TCACCAGGGA 
CCAGAGTGGC TATGGCCGAT CGGCTACTTT TTGCGAGCGC GCTTGCACTA CGCGCCCCTC 
GTTGGTGGCG AAGAGGAGTT AAAACGGACT ATCGCCTCGA CCGAAGCTAT CATCTCGAGA 
CATCTGACCG AAGCTTCAAA CAACCACTGG CGTGGACTTC CCGAGTTAAC CAATCGCGAG 
GGCGAGTATT GCGCTGATAG CTGTCGCACT CAGGCCTGGA GCGCTTCCGC AATTGTCGAG 
GTCCTCCACG ATTTGGACAA GCTGAAAAAG GATCTGAAGT TTGACGACGA GCAGGTCCTC 
ACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100117168 ( XM_008219351.2 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_008217573.1 Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X4, mRNA.

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CloneID	ONd11608
Clone ID Related Accession (Same CDS sequence)	XM_008219351.2
Accession Version	XM_008219351.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-25
Organism	Nasonia vitripennis(jewel wasp)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001815014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2016 this sequence version replaced XM_008219351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGCTC TGCAGAATCT AGTGTGTGAC TCCACACGAC AATTATTCAA GAAGATAGGG 
TATTTCTTTC ACCGCTGCTT GACCTTATTT TGGTCTTATT TACATCTGCA ACGTGCAATC 
GACGATTCCA AAATTACACC CAAAAAGAAG ACCATGGAAA ATCTCGGCTT CTCTGCGGAT 
ACCCAGAACG ACGACCAAGT GCGAGTACTG ACTCTGAATA AGGGAGAGCA CCAGGATGGG 
ACCCTGTACA AGCTTAAAAA AGGATGGGTC GTGGAGTTCA GATTAGGGGC ATCGCTTCTG 
GGCCACAATC TCAACATATT CACTAATCAT CCTCTACTCG TCGGCAACCC GTTCGAGAGG 
CGCACGTACT ATCAGCTGCC ATGGAATCAC GAGTCAGCTT TCATACGCGT CTCGCTGGCC 
GGCTCTTTTC ATTTTTATCT GGCAGAAGCG AGTGGAGAGG ACGCCGGAAA AGTGATAGCT 
TCCGGCTACT TGTTGGTCGA TCCGGAGCTA ACCGTGGGCG TCAACAACCA GCCGCTGCCG 
CTAGACTGCA TACAATGCCA GACGGTCCTC TCAAAGCTCA TGGGACCCTT CTCAACGTGG 
GAGGAGAAGC TCAGGGTCTC TCGCAAGTCA GGCTACAACA TGATCCACTT CACACCGATA 
CAGGCGATAG GAGACTCAAA ATCAGCTTAC AGCCTCAGCG ATCAGCTGAA GCTCAACCCC 
TCGTTCAATG ATGCACCGAA CAAAGAGACC AGCTTCGACG ACGTCGAGAA GTTGATTACA 
AAAATGAGAG AAGAGTGGAA CACTGTGAGC ATTTGCGACA TAGTTTTGAA TCACACGGCG 
AACGAGAGTC CATTTTTGAT AAACAATCCC GAATGTAGTT ACAACTGCAA CAACTGTCCA 
CATCTCCGTC CGTCTTATCT TTTGGATGCC ATTTTATTCG AGTTGACAAT ACGAGTGTCG 
TCCGGAGAAT GGGAGCACAA AGGAATTCCT CCCGTCGTCG AGACTGAAGA GCACTTGAAC 
GCAATCCGTC ATGCTCTGCA CACTTACTTT CTACCTCTGG CGAAAATTCA CGAACTGTAT 
ACTGTAGATA TCAACGAGAT CGTGTCCGAG TTTTTGAACA TTGCTCGCAA CCAGATACCG 
CAAGACGAGC CACCCGAAAC CCATAACGAA AGCATCGTTA TCATAAGGGA TCCAGAATAT 
CGCCGCTTAA AGTCCACTAT AGATATGCAG CTTGCTTTGA AGATCTACAA TATTTATAGA 
CCCGATTGTT TTGACGAGGA CTCACGACTG AAACGCTGCG CGGAAGAACT GAGAAACAAG 
TTGCAGGAGT TAAACGATGC CGCAACTAAC GAAATCCAAA ATCATCTCAA TGCCGCGGTT 
GAAAACTCGG TAGCCGGCAT TCGATACTTC AGAGTCCAAG CAGATGGGCC AAGACAGAAA 
GAGATAAGCG AAAGAAATCC ACTTGTTCCT AGGTATTTCA CGGATTACGG AAGTCCTAGA 
TCGTTTGTGG AAAGAGAAGA CATCATGTAC TCGAATTCTG GATGTTACTT GATGGCTCAC 
AACGGCTGGG TCATGAACAG CGATCCCTTG AAAAACTTTG CCGATGCTGA CTCTCATGTT 
TACATAAGAA GGGAATTGAT TGCGTGGGGC GATAGTGTCA AATTAAGATA TGGCGAAAAA 
CCGGAAGATT GCCCTGCTCT TTGGGAACGT ATGACGACAT ATGTTCAGCA AATGGCTAAA 
ATCTTCGACG GCGTCAGACT CGACAATTGT CACTCGACCC CTATTCCAGT AGCAGAACAC 
ATGTTGGATG CTGCTCGTCA AGTAAGACCA GATTTGTACG TTGCCGCTGA ACTCTTCACC 
AACTCCGATC AGAAAGATAA TATTTTCGTC AATCGTCTTG GAATCACATC GTTGATAAGA 
GAGGCCATGT CAGCGTGGGA CAGCCATGAG GAAGGCCGTC TGGTATACCG CTATGGTGGC 
GAGCCCGTAG GTGCGTTTAT CCAGGCTCGA CATCGTCCTC TGGCACCGAG TATCGCGCAT 
GCTTTATTCC TTGATCAGAC GCATGACAAC CCGAGCCCCA TCGAGAAGCG TAGCGTATTT 
GACTTATTGC CCAGTGCCGC ACTGGTCTCC ATGGCCTGCT GTGCTAGTGG CAGTAATCGC 
GGTTACGACG AGCTCGTGCC TCATCATATT CACGTTGTCG ATGAAGACAG ACAATATACT 
TCTTGGACAG ATAAGGAAGG ACTGGCAAAG GAAAATGCAA AGTATGTTAG CAAAAAATCG 
GGAATCATTT CTGCTAAAAA GGCTCTCAAT GATCTCCATT TTTGGCTGGG ACAGAACTCC 
TTTTCTCAAG TTTTTGTCGA TCAAATGGAT GTTGATGTGG TAGCGGTTAC AAGACATTCA 
CCGACCACTC ACGAGAGCGT CGTACTCGTG GCTTTTACGG CATTCAAGCA CCCGGATCCC 
AATGCTAGCG ATTTGAGAAG ACATGTTAGA CCTTTGAGGG TAGAAGGCGT AGTAGATGAA 
ATTATTCTTG AAGCATCCCT CGCGCACGGA GAAAAAGAAA ATGGTATCCC TCCGTTCTCT 
AATCCGAAGA AATACGTAAA GAACGAAGAC TACATAAACG GTTACTCTGA GTACTTGGTG 
GACGTCAAGG AGCACATTCA AATCTGTGAT TCTACAAAAG TTGAAAAGGT CGATTCCGGA 
GATCCTAAAA TAACGCAATT GAACTTTATT AATTTCCAGC CCGGATCAAT AATCACTATT 
CGGGTGTCAC TCCACGCAAA CATAAAGCCA GCTCTTGAAT CCATCAATGA CCTTATCTCG 
ACCCTCACAT CTCCGAACAA TTCGGAATTG CGTGCAATTG TTTCGCGCAT GAACTTTGCG 
GACCTTAACA AAGCACTTTA TAGGTGTGAT CAAGAAGAGC GAGATACGAC TGCCAATAAA 
TTTGGAGTTT ATGATATTCC CGGTTATGGC CCACTAGTTT ACGCTGGTTT GCAAGGTGTA 
ATTTCATTGC TTGCTGATAT CAGATCGAAC AACGATCTTG GTCATCCACT ATGTGGCAAC 
CTTCGTCAAG GCAATTGGTT GATAGATTAT ACTTGGCAAC GTTTGAAGGA AGATGAAGGT 
ACTGCAGCTC TTGGAGAGTG GCTGGAGCAG GCCTGCGAAC CATTCAAGCT AATTCCTCGT 
TATTTAGTGC CCAGTTACTT TGACGTGATC ATCGTTAACG TGTATTCTAC TCTCTTAGAT 
CAATGCTTTA ATCTCATGTC TAGCTTTGTG AAAGATGGAA CCGATTTCAC CAGACTGCTG 
TCACTAGTAT CTGTGCAAGT TGGCGGTATA ATAAAATCAG CGCAATTGCC AGATTTATCA 
CCGAACCTAG ATCCACCGAA ACCTAGCATA CGAGAGGTGG ATGGCAAATC AGAACAGGAA 
TGTTTGACCA TGTCTGCTGG ACTACCACAC TTTACAGTAG GATACATGAG AAACTGGGGA 
CGAGATACGT TTATCGCTTT GAGAGGTCTG TTCTTATTAA CCGGCAGACA TGCGGAGGCT 
CGATTCATCA TTCTGGGATA CGCCGCTACG CTTCGTCACG GTTTGATACC AAATCTCTTG 
GACAGAGGCG TCAACGCCAG ATTCAATTGC CGAGATGCCG TTTGGTGGTG GTTGTACACT 
ATTCAATGCT ATGTCAAAGA AGTCCCAAAT GGCTTAAAAC TTCTGTCGGA CAAAGTATCT 
AGATTGTTCC CTACTGACGA TTCGGACGCT CTTCCAGCCG GAGAAGTGGA TGAAACACTG 
TCGGATGTCA TGCAGGAAGC ACTGACAGTT CACTTTCAAG GTCTGTGCTT CCGTGAGCGC 
AACGCCGGTA AGCGCATCGA CGAGCACATG ACTGATCGTG GATTCAACAA TCAGATAGGT 
GTGCATCCAG AGACGGGCTT TGTATTTGGC GGTAACGAGG CCAATTGCGG CACTTGGATG 
GACAAGATGG GCTCCTCGGA AAAGGCAGGA AACAAAGGCA AACCTGCCAC TCCCAGAGAC 
GGATCTGCGG TTGAAATTAT TGGGCTCTGT CACAGCACTC TCAATTTTCT GGCAGAATTG 
TACAAACAGA ATCTATTTCC ATATGGTAGC GTGCAGCGGA AAAATCGCGA TGGCTCAACG 
ATAACTTGGA GTTACAAACA GTGGGCGGAT AAGATAAAGG AGAGTTTCGA TACTTACTTC 
TATGTGAACG AGAAACCTAC CGATGGTGAA TTACGACCGG ACTTGATTCA TCGACGAGGA 
ATTTACAAAG ATACTCACGG AGCCTCACAA CCGTGGGCAG ATTATCAATT GCGACCTAAC 
TTCCCAGTGG CTATGGTTGC TTCGCCGAAC CTATTCAAGC CCGAGCACGC CTGGCTGGCA 
TTAAAGAAGG CCGAAGAAAT CCTCCTTGGT CCCCTAGGCA TGAAAACCCT AGATCCCGCT 
GACTGGGCCT ACGATGGCTT CTACAACAAT GCCAACGACT CGAATGACCC GAAAGTAGCT 
CACGGATGGA ATTATCACCA GGGACCAGAG TGGCTATGGC CGATCGGCTA CTTTTTGCGA 
GCGCGCTTGC ACTACGCGCC CCTCGTTGGT GGCGAAGAGG AGTTAAAACG GACTATCGCC 
TCGACCGAAG CTATCATCTC GAGACATCTG ACCGAAGCTT CAAACAACCA CTGGCGTGGA 
CTTCCCGAGT TAACCAATCG CGAGGGCGAG TATTGCGCTG ATAGCTGTCG CACTCAGGCC 
TGGAGCGCTT CCGCAATTGT CGAGGTCCTC CACGATTTGG ACAAGCTGAA AAAGGATCTG 
AAGTTTGACG ACGAGCAGGT CCTCACGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008217573.1
CDS	655..5487
Translation	MVALQNLVCDSTRQLFKKIGYFFHRCLTLFWSYLHLQRAIDDSKITPKKKTMENLGFSADTQNDDQVRVLTLNKGEHQDGTLYKLKKGWVVEFRLGASLLGHNLNIFTNHPLLVGNPFERRTYYQLPWNHESAFIRVSLAGSFHFYLAEASGEDAGKVIASGYLLVDPELTVGVNNQPLPLDCIQCQTVLSKLMGPFSTWEEKLRVSRKSGYNMIHFTPIQAIGDSKSAYSLSDQLKLNPSFNDAPNKETSFDDVEKLITKMREEWNTVSICDIVLNHTANESPFLINNPECSYNCNNCPHLRPSYLLDAILFELTIRVSSGEWEHKGIPPVVETEEHLNAIRHALHTYFLPLAKIHELYTVDINEIVSEFLNIARNQIPQDEPPETHNESIVIIRDPEYRRLKSTIDMQLALKIYNIYRPDCFDEDSRLKRCAEELRNKLQELNDAATNEIQNHLNAAVENSVAGIRYFRVQADGPRQKEISERNPLVPRYFTDYGSPRSFVEREDIMYSNSGCYLMAHNGWVMNSDPLKNFADADSHVYIRRELIAWGDSVKLRYGEKPEDCPALWERMTTYVQQMAKIFDGVRLDNCHSTPIPVAEHMLDAARQVRPDLYVAAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFIQARHRPLAPSIAHALFLDQTHDNPSPIEKRSVFDLLPSAALVSMACCASGSNRGYDELVPHHIHVVDEDRQYTSWTDKEGLAKENAKYVSKKSGIISAKKALNDLHFWLGQNSFSQVFVDQMDVDVVAVTRHSPTTHESVVLVAFTAFKHPDPNASDLRRHVRPLRVEGVVDEIILEASLAHGEKENGIPPFSNPKKYVKNEDYINGYSEYLVDVKEHIQICDSTKVEKVDSGDPKITQLNFINFQPGSIITIRVSLHANIKPALESINDLISTLTSPNNSELRAIVSRMNFADLNKALYRCDQEERDTTANKFGVYDIPGYGPLVYAGLQGVISLLADIRSNNDLGHPLCGNLRQGNWLIDYTWQRLKEDEGTAALGEWLEQACEPFKLIPRYLVPSYFDVIIVNVYSTLLDQCFNLMSSFVKDGTDFTRLLSLVSVQVGGIIKSAQLPDLSPNLDPPKPSIREVDGKSEQECLTMSAGLPHFTVGYMRNWGRDTFIALRGLFLLTGRHAEARFIILGYAATLRHGLIPNLLDRGVNARFNCRDAVWWWLYTIQCYVKEVPNGLKLLSDKVSRLFPTDDSDALPAGEVDETLSDVMQEALTVHFQGLCFRERNAGKRIDEHMTDRGFNNQIGVHPETGFVFGGNEANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIIGLCHSTLNFLAELYKQNLFPYGSVQRKNRDGSTITWSYKQWADKIKESFDTYFYVNEKPTDGELRPDLIHRRGIYKDTHGASQPWADYQLRPNFPVAMVASPNLFKPEHAWLALKKAEEILLGPLGMKTLDPADWAYDGFYNNANDSNDPKVAHGWNYHQGPEWLWPIGYFLRARLHYAPLVGGEEELKRTIASTEAIISRHLTEASNNHWRGLPELTNREGEYCADSCRTQAWSASAIVEVLHDLDKLKKDLKFDDEQVLTN

Target ORF information:

RefSeq Version	XM_008219351.2
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008219351.2

ORF Insert Sequence:

ATGGTTGCTC TGCAGAATCT AGTGTGTGAC TCCACACGAC AATTATTCAA GAAGATAGGG 
TATTTCTTTC ACCGCTGCTT GACCTTATTT TGGTCTTATT TACATCTGCA ACGTGCAATC 
GACGATTCCA AAATTACACC CAAAAAGAAG ACCATGGAAA ATCTCGGCTT CTCTGCGGAT 
ACCCAGAACG ACGACCAAGT GCGAGTACTG ACTCTGAATA AGGGAGAGCA CCAGGATGGG 
ACCCTGTACA AGCTTAAAAA AGGATGGGTC GTGGAGTTCA GATTAGGGGC ATCGCTTCTG 
GGCCACAATC TCAACATATT CACTAATCAT CCTCTACTCG TCGGCAACCC GTTCGAGAGG 
CGCACGTACT ATCAGCTGCC ATGGAATCAC GAGTCAGCTT TCATACGCGT CTCGCTGGCC 
GGCTCTTTTC ATTTTTATCT GGCAGAAGCG AGTGGAGAGG ACGCCGGAAA AGTGATAGCT 
TCCGGCTACT TGTTGGTCGA TCCGGAGCTA ACCGTGGGCG TCAACAACCA GCCGCTGCCG 
CTAGACTGCA TACAATGCCA GACGGTCCTC TCAAAGCTCA TGGGACCCTT CTCAACGTGG 
GAGGAGAAGC TCAGGGTCTC TCGCAAGTCA GGCTACAACA TGATCCACTT CACACCGATA 
CAGGCGATAG GAGACTCAAA ATCAGCTTAC AGCCTCAGCG ATCAGCTGAA GCTCAACCCC 
TCGTTCAATG ATGCACCGAA CAAAGAGACC AGCTTCGACG ACGTCGAGAA GTTGATTACA 
AAAATGAGAG AAGAGTGGAA CACTGTGAGC ATTTGCGACA TAGTTTTGAA TCACACGGCG 
AACGAGAGTC CATTTTTGAT AAACAATCCC GAATGTAGTT ACAACTGCAA CAACTGTCCA 
CATCTCCGTC CGTCTTATCT TTTGGATGCC ATTTTATTCG AGTTGACAAT ACGAGTGTCG 
TCCGGAGAAT GGGAGCACAA AGGAATTCCT CCCGTCGTCG AGACTGAAGA GCACTTGAAC 
GCAATCCGTC ATGCTCTGCA CACTTACTTT CTACCTCTGG CGAAAATTCA CGAACTGTAT 
ACTGTAGATA TCAACGAGAT CGTGTCCGAG TTTTTGAACA TTGCTCGCAA CCAGATACCG 
CAAGACGAGC CACCCGAAAC CCATAACGAA AGCATCGTTA TCATAAGGGA TCCAGAATAT 
CGCCGCTTAA AGTCCACTAT AGATATGCAG CTTGCTTTGA AGATCTACAA TATTTATAGA 
CCCGATTGTT TTGACGAGGA CTCACGACTG AAACGCTGCG CGGAAGAACT GAGAAACAAG 
TTGCAGGAGT TAAACGATGC CGCAACTAAC GAAATCCAAA ATCATCTCAA TGCCGCGGTT 
GAAAACTCGG TAGCCGGCAT TCGATACTTC AGAGTCCAAG CAGATGGGCC AAGACAGAAA 
GAGATAAGCG AAAGAAATCC ACTTGTTCCT AGGTATTTCA CGGATTACGG AAGTCCTAGA 
TCGTTTGTGG AAAGAGAAGA CATCATGTAC TCGAATTCTG GATGTTACTT GATGGCTCAC 
AACGGCTGGG TCATGAACAG CGATCCCTTG AAAAACTTTG CCGATGCTGA CTCTCATGTT 
TACATAAGAA GGGAATTGAT TGCGTGGGGC GATAGTGTCA AATTAAGATA TGGCGAAAAA 
CCGGAAGATT GCCCTGCTCT TTGGGAACGT ATGACGACAT ATGTTCAGCA AATGGCTAAA 
ATCTTCGACG GCGTCAGACT CGACAATTGT CACTCGACCC CTATTCCAGT AGCAGAACAC 
ATGTTGGATG CTGCTCGTCA AGTAAGACCA GATTTGTACG TTGCCGCTGA ACTCTTCACC 
AACTCCGATC AGAAAGATAA TATTTTCGTC AATCGTCTTG GAATCACATC GTTGATAAGA 
GAGGCCATGT CAGCGTGGGA CAGCCATGAG GAAGGCCGTC TGGTATACCG CTATGGTGGC 
GAGCCCGTAG GTGCGTTTAT CCAGGCTCGA CATCGTCCTC TGGCACCGAG TATCGCGCAT 
GCTTTATTCC TTGATCAGAC GCATGACAAC CCGAGCCCCA TCGAGAAGCG TAGCGTATTT 
GACTTATTGC CCAGTGCCGC ACTGGTCTCC ATGGCCTGCT GTGCTAGTGG CAGTAATCGC 
GGTTACGACG AGCTCGTGCC TCATCATATT CACGTTGTCG ATGAAGACAG ACAATATACT 
TCTTGGACAG ATAAGGAAGG ACTGGCAAAG GAAAATGCAA AGTATGTTAG CAAAAAATCG 
GGAATCATTT CTGCTAAAAA GGCTCTCAAT GATCTCCATT TTTGGCTGGG ACAGAACTCC 
TTTTCTCAAG TTTTTGTCGA TCAAATGGAT GTTGATGTGG TAGCGGTTAC AAGACATTCA 
CCGACCACTC ACGAGAGCGT CGTACTCGTG GCTTTTACGG CATTCAAGCA CCCGGATCCC 
AATGCTAGCG ATTTGAGAAG ACATGTTAGA CCTTTGAGGG TAGAAGGCGT AGTAGATGAA 
ATTATTCTTG AAGCATCCCT CGCGCACGGA GAAAAAGAAA ATGGTATCCC TCCGTTCTCT 
AATCCGAAGA AATACGTAAA GAACGAAGAC TACATAAACG GTTACTCTGA GTACTTGGTG 
GACGTCAAGG AGCACATTCA AATCTGTGAT TCTACAAAAG TTGAAAAGGT CGATTCCGGA 
GATCCTAAAA TAACGCAATT GAACTTTATT AATTTCCAGC CCGGATCAAT AATCACTATT 
CGGGTGTCAC TCCACGCAAA CATAAAGCCA GCTCTTGAAT CCATCAATGA CCTTATCTCG 
ACCCTCACAT CTCCGAACAA TTCGGAATTG CGTGCAATTG TTTCGCGCAT GAACTTTGCG 
GACCTTAACA AAGCACTTTA TAGGTGTGAT CAAGAAGAGC GAGATACGAC TGCCAATAAA 
TTTGGAGTTT ATGATATTCC CGGTTATGGC CCACTAGTTT ACGCTGGTTT GCAAGGTGTA 
ATTTCATTGC TTGCTGATAT CAGATCGAAC AACGATCTTG GTCATCCACT ATGTGGCAAC 
CTTCGTCAAG GCAATTGGTT GATAGATTAT ACTTGGCAAC GTTTGAAGGA AGATGAAGGT 
ACTGCAGCTC TTGGAGAGTG GCTGGAGCAG GCCTGCGAAC CATTCAAGCT AATTCCTCGT 
TATTTAGTGC CCAGTTACTT TGACGTGATC ATCGTTAACG TGTATTCTAC TCTCTTAGAT 
CAATGCTTTA ATCTCATGTC TAGCTTTGTG AAAGATGGAA CCGATTTCAC CAGACTGCTG 
TCACTAGTAT CTGTGCAAGT TGGCGGTATA ATAAAATCAG CGCAATTGCC AGATTTATCA 
CCGAACCTAG ATCCACCGAA ACCTAGCATA CGAGAGGTGG ATGGCAAATC AGAACAGGAA 
TGTTTGACCA TGTCTGCTGG ACTACCACAC TTTACAGTAG GATACATGAG AAACTGGGGA 
CGAGATACGT TTATCGCTTT GAGAGGTCTG TTCTTATTAA CCGGCAGACA TGCGGAGGCT 
CGATTCATCA TTCTGGGATA CGCCGCTACG CTTCGTCACG GTTTGATACC AAATCTCTTG 
GACAGAGGCG TCAACGCCAG ATTCAATTGC CGAGATGCCG TTTGGTGGTG GTTGTACACT 
ATTCAATGCT ATGTCAAAGA AGTCCCAAAT GGCTTAAAAC TTCTGTCGGA CAAAGTATCT 
AGATTGTTCC CTACTGACGA TTCGGACGCT CTTCCAGCCG GAGAAGTGGA TGAAACACTG 
TCGGATGTCA TGCAGGAAGC ACTGACAGTT CACTTTCAAG GTCTGTGCTT CCGTGAGCGC 
AACGCCGGTA AGCGCATCGA CGAGCACATG ACTGATCGTG GATTCAACAA TCAGATAGGT 
GTGCATCCAG AGACGGGCTT TGTATTTGGC GGTAACGAGG CCAATTGCGG CACTTGGATG 
GACAAGATGG GCTCCTCGGA AAAGGCAGGA AACAAAGGCA AACCTGCCAC TCCCAGAGAC 
GGATCTGCGG TTGAAATTAT TGGGCTCTGT CACAGCACTC TCAATTTTCT GGCAGAATTG 
TACAAACAGA ATCTATTTCC ATATGGTAGC GTGCAGCGGA AAAATCGCGA TGGCTCAACG 
ATAACTTGGA GTTACAAACA GTGGGCGGAT AAGATAAAGG AGAGTTTCGA TACTTACTTC 
TATGTGAACG AGAAACCTAC CGATGGTGAA TTACGACCGG ACTTGATTCA TCGACGAGGA 
ATTTACAAAG ATACTCACGG AGCCTCACAA CCGTGGGCAG ATTATCAATT GCGACCTAAC 
TTCCCAGTGG CTATGGTTGC TTCGCCGAAC CTATTCAAGC CCGAGCACGC CTGGCTGGCA 
TTAAAGAAGG CCGAAGAAAT CCTCCTTGGT CCCCTAGGCA TGAAAACCCT AGATCCCGCT 
GACTGGGCCT ACGATGGCTT CTACAACAAT GCCAACGACT CGAATGACCC GAAAGTAGCT 
CACGGATGGA ATTATCACCA GGGACCAGAG TGGCTATGGC CGATCGGCTA CTTTTTGCGA 
GCGCGCTTGC ACTACGCGCC CCTCGTTGGT GGCGAAGAGG AGTTAAAACG GACTATCGCC 
TCGACCGAAG CTATCATCTC GAGACATCTG ACCGAAGCTT CAAACAACCA CTGGCGTGGA 
CTTCCCGAGT TAACCAATCG CGAGGGCGAG TATTGCGCTG ATAGCTGTCG CACTCAGGCC 
TGGAGCGCTT CCGCAATTGT CGAGGTCCTC CACGATTTGG ACAAGCTGAA AAAGGATCTG 
AAGTTTGACG ACGAGCAGGT CCTCACGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100117168 ( XM_008219354.2 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_008217576.1 Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X5, mRNA.

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CloneID	ONd11609
Clone ID Related Accession (Same CDS sequence)	XM_008219354.2
Accession Version	XM_008219354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-25
Organism	Nasonia vitripennis(jewel wasp)
Product	glycogen debranching enzyme isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001815014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2016 this sequence version replaced XM_008219354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGCTC TGCAGAATCT AGTGTGTGAC TCCACACGAC AATTATTCAA GAAGATAGGG 
TATTTCTTTC ACCGCTGCTT GACCTTATTT TGGTCTTATC CCAAAAAGAA GACCATGGAA 
AATCTCGGCT TCTCTGCGGA TACCCAGAAC GACGACCAAG TGCGAGTACT GACTCTGAAT 
AAGGGAGAGC ACCAGGATGG GACCCTGTAC AAGCTTAAAA AAGGATGGGT CGTGGAGTTC 
AGATTAGGGG CATCGCTTCT GGGCCACAAT CTCAACATAT TCACTAATCA TCCTCTACTC 
GTCGGCAACC CGTTCGAGAG GCGCACGTAC TATCAGCTGC CATGGAATCA CGAGTCAGCT 
TTCATACGCG TCTCGCTGGC CGGCTCTTTT CATTTTTATC TGGCAGAAGC GAGTGGAGAG 
GACGCCGGAA AAGTGATAGC TTCCGGCTAC TTGTTGGTCG ATCCGGAGCT AACCGTGGGC 
GTCAACAACC AGCCGCTGCC GCTAGACTGC ATACAATGCC AGACGGTCCT CTCAAAGCTC 
ATGGGACCCT TCTCAACGTG GGAGGAGAAG CTCAGGGTCT CTCGCAAGTC AGGCTACAAC 
ATGATCCACT TCACACCGAT ACAGGCGATA GGAGACTCAA AATCAGCTTA CAGCCTCAGC 
GATCAGCTGA AGCTCAACCC CTCGTTCAAT GATGCACCGA ACAAAGAGAC CAGCTTCGAC 
GACGTCGAGA AGTTGATTAC AAAAATGAGA GAAGAGTGGA ACACTGTGAG CATTTGCGAC 
ATAGTTTTGA ATCACACGGC GAACGAGAGT CCATTTTTGA TAAACAATCC CGAATGTAGT 
TACAACTGCA ACAACTGTCC ACATCTCCGT CCGTCTTATC TTTTGGATGC CATTTTATTC 
GAGTTGACAA TACGAGTGTC GTCCGGAGAA TGGGAGCACA AAGGAATTCC TCCCGTCGTC 
GAGACTGAAG AGCACTTGAA CGCAATCCGT CATGCTCTGC ACACTTACTT TCTACCTCTG 
GCGAAAATTC ACGAACTGTA TACTGTAGAT ATCAACGAGA TCGTGTCCGA GTTTTTGAAC 
ATTGCTCGCA ACCAGATACC GCAAGACGAG CCACCCGAAA CCCATAACGA AAGCATCGTT 
ATCATAAGGG ATCCAGAATA TCGCCGCTTA AAGTCCACTA TAGATATGCA GCTTGCTTTG 
AAGATCTACA ATATTTATAG ACCCGATTGT TTTGACGAGG ACTCACGACT GAAACGCTGC 
GCGGAAGAAC TGAGAAACAA GTTGCAGGAG TTAAACGATG CCGCAACTAA CGAAATCCAA 
AATCATCTCA ATGCCGCGGT TGAAAACTCG GTAGCCGGCA TTCGATACTT CAGAGTCCAA 
GCAGATGGGC CAAGACAGAA AGAGATAAGC GAAAGAAATC CACTTGTTCC TAGGTATTTC 
ACGGATTACG GAAGTCCTAG ATCGTTTGTG GAAAGAGAAG ACATCATGTA CTCGAATTCT 
GGATGTTACT TGATGGCTCA CAACGGCTGG GTCATGAACA GCGATCCCTT GAAAAACTTT 
GCCGATGCTG ACTCTCATGT TTACATAAGA AGGGAATTGA TTGCGTGGGG CGATAGTGTC 
AAATTAAGAT ATGGCGAAAA ACCGGAAGAT TGCCCTGCTC TTTGGGAACG TATGACGACA 
TATGTTCAGC AAATGGCTAA AATCTTCGAC GGCGTCAGAC TCGACAATTG TCACTCGACC 
CCTATTCCAG TAGCAGAACA CATGTTGGAT GCTGCTCGTC AAGTAAGACC AGATTTGTAC 
GTTGCCGCTG AACTCTTCAC CAACTCCGAT CAGAAAGATA ATATTTTCGT CAATCGTCTT 
GGAATCACAT CGTTGATAAG AGAGGCCATG TCAGCGTGGG ACAGCCATGA GGAAGGCCGT 
CTGGTATACC GCTATGGTGG CGAGCCCGTA GGTGCGTTTA TCCAGGCTCG ACATCGTCCT 
CTGGCACCGA GTATCGCGCA TGCTTTATTC CTTGATCAGA CGCATGACAA CCCGAGCCCC 
ATCGAGAAGC GTAGCGTATT TGACTTATTG CCCAGTGCCG CACTGGTCTC CATGGCCTGC 
TGTGCTAGTG GCAGTAATCG CGGTTACGAC GAGCTCGTGC CTCATCATAT TCACGTTGTC 
GATGAAGACA GACAATATAC TTCTTGGACA GATAAGGAAG GACTGGCAAA GGAAAATGCA 
AAGTATGTTA GCAAAAAATC GGGAATCATT TCTGCTAAAA AGGCTCTCAA TGATCTCCAT 
TTTTGGCTGG GACAGAACTC CTTTTCTCAA GTTTTTGTCG ATCAAATGGA TGTTGATGTG 
GTAGCGGTTA CAAGACATTC ACCGACCACT CACGAGAGCG TCGTACTCGT GGCTTTTACG 
GCATTCAAGC ACCCGGATCC CAATGCTAGC GATTTGAGAA GACATGTTAG ACCTTTGAGG 
GTAGAAGGCG TAGTAGATGA AATTATTCTT GAAGCATCCC TCGCGCACGG AGAAAAAGAA 
AATGGTATCC CTCCGTTCTC TAATCCGAAG AAATACGTAA AGAACGAAGA CTACATAAAC 
GGTTACTCTG AGTACTTGGT GGACGTCAAG GAGCACATTC AAATCTGTGA TTCTACAAAA 
GTTGAAAAGG TCGATTCCGG AGATCCTAAA ATAACGCAAT TGAACTTTAT TAATTTCCAG 
CCCGGATCAA TAATCACTAT TCGGGTGTCA CTCCACGCAA ACATAAAGCC AGCTCTTGAA 
TCCATCAATG ACCTTATCTC GACCCTCACA TCTCCGAACA ATTCGGAATT GCGTGCAATT 
GTTTCGCGCA TGAACTTTGC GGACCTTAAC AAAGCACTTT ATAGGTGTGA TCAAGAAGAG 
CGAGATACGA CTGCCAATAA ATTTGGAGTT TATGATATTC CCGGTTATGG CCCACTAGTT 
TACGCTGGTT TGCAAGGTGT AATTTCATTG CTTGCTGATA TCAGATCGAA CAACGATCTT 
GGTCATCCAC TATGTGGCAA CCTTCGTCAA GGCAATTGGT TGATAGATTA TACTTGGCAA 
CGTTTGAAGG AAGATGAAGG TACTGCAGCT CTTGGAGAGT GGCTGGAGCA GGCCTGCGAA 
CCATTCAAGC TAATTCCTCG TTATTTAGTG CCCAGTTACT TTGACGTGAT CATCGTTAAC 
GTGTATTCTA CTCTCTTAGA TCAATGCTTT AATCTCATGT CTAGCTTTGT GAAAGATGGA 
ACCGATTTCA CCAGACTGCT GTCACTAGTA TCTGTGCAAG TTGGCGGTAT AATAAAATCA 
GCGCAATTGC CAGATTTATC ACCGAACCTA GATCCACCGA AACCTAGCAT ACGAGAGGTG 
GATGGCAAAT CAGAACAGGA ATGTTTGACC ATGTCTGCTG GACTACCACA CTTTACAGTA 
GGATACATGA GAAACTGGGG ACGAGATACG TTTATCGCTT TGAGAGGTCT GTTCTTATTA 
ACCGGCAGAC ATGCGGAGGC TCGATTCATC ATTCTGGGAT ACGCCGCTAC GCTTCGTCAC 
GGTTTGATAC CAAATCTCTT GGACAGAGGC GTCAACGCCA GATTCAATTG CCGAGATGCC 
GTTTGGTGGT GGTTGTACAC TATTCAATGC TATGTCAAAG AAGTCCCAAA TGGCTTAAAA 
CTTCTGTCGG ACAAAGTATC TAGATTGTTC CCTACTGACG ATTCGGACGC TCTTCCAGCC 
GGAGAAGTGG ATGAAACACT GTCGGATGTC ATGCAGGAAG CACTGACAGT TCACTTTCAA 
GGTCTGTGCT TCCGTGAGCG CAACGCCGGT AAGCGCATCG ACGAGCACAT GACTGATCGT 
GGATTCAACA ATCAGATAGG TGTGCATCCA GAGACGGGCT TTGTATTTGG CGGTAACGAG 
GCCAATTGCG GCACTTGGAT GGACAAGATG GGCTCCTCGG AAAAGGCAGG AAACAAAGGC 
AAACCTGCCA CTCCCAGAGA CGGATCTGCG GTTGAAATTA TTGGGCTCTG TCACAGCACT 
CTCAATTTTC TGGCAGAATT GTACAAACAG AATCTATTTC CATATGGTAG CGTGCAGCGG 
AAAAATCGCG ATGGCTCAAC GATAACTTGG AGTTACAAAC AGTGGGCGGA TAAGATAAAG 
GAGAGTTTCG ATACTTACTT CTATGTGAAC GAGAAACCTA CCGATGGTGA ATTACGACCG 
GACTTGATTC ATCGACGAGG AATTTACAAA GATACTCACG GAGCCTCACA ACCGTGGGCA 
GATTATCAAT TGCGACCTAA CTTCCCAGTG GCTATGGTTG CTTCGCCGAA CCTATTCAAG 
CCCGAGCACG CCTGGCTGGC ATTAAAGAAG GCCGAAGAAA TCCTCCTTGG TCCCCTAGGC 
ATGAAAACCC TAGATCCCGC TGACTGGGCC TACGATGGCT TCTACAACAA TGCCAACGAC 
TCGAATGACC CGAAAGTAGC TCACGGATGG AATTATCACC AGGGACCAGA GTGGCTATGG 
CCGATCGGCT ACTTTTTGCG AGCGCGCTTG CACTACGCGC CCCTCGTTGG TGGCGAAGAG 
GAGTTAAAAC GGACTATCGC CTCGACCGAA GCTATCATCT CGAGACATCT GACCGAAGCT 
TCAAACAACC ACTGGCGTGG ACTTCCCGAG TTAACCAATC GCGAGGGCGA GTATTGCGCT 
GATAGCTGTC GCACTCAGGC CTGGAGCGCT TCCGCAATTG TCGAGGTCCT CCACGATTTG 
GACAAGCTGA AAAAGGATCT GAAGTTTGAC GACGAGCAGG TCCTCACGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008217576.1
CDS	654..5447
Translation	MVALQNLVCDSTRQLFKKIGYFFHRCLTLFWSYPKKKTMENLGFSADTQNDDQVRVLTLNKGEHQDGTLYKLKKGWVVEFRLGASLLGHNLNIFTNHPLLVGNPFERRTYYQLPWNHESAFIRVSLAGSFHFYLAEASGEDAGKVIASGYLLVDPELTVGVNNQPLPLDCIQCQTVLSKLMGPFSTWEEKLRVSRKSGYNMIHFTPIQAIGDSKSAYSLSDQLKLNPSFNDAPNKETSFDDVEKLITKMREEWNTVSICDIVLNHTANESPFLINNPECSYNCNNCPHLRPSYLLDAILFELTIRVSSGEWEHKGIPPVVETEEHLNAIRHALHTYFLPLAKIHELYTVDINEIVSEFLNIARNQIPQDEPPETHNESIVIIRDPEYRRLKSTIDMQLALKIYNIYRPDCFDEDSRLKRCAEELRNKLQELNDAATNEIQNHLNAAVENSVAGIRYFRVQADGPRQKEISERNPLVPRYFTDYGSPRSFVEREDIMYSNSGCYLMAHNGWVMNSDPLKNFADADSHVYIRRELIAWGDSVKLRYGEKPEDCPALWERMTTYVQQMAKIFDGVRLDNCHSTPIPVAEHMLDAARQVRPDLYVAAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFIQARHRPLAPSIAHALFLDQTHDNPSPIEKRSVFDLLPSAALVSMACCASGSNRGYDELVPHHIHVVDEDRQYTSWTDKEGLAKENAKYVSKKSGIISAKKALNDLHFWLGQNSFSQVFVDQMDVDVVAVTRHSPTTHESVVLVAFTAFKHPDPNASDLRRHVRPLRVEGVVDEIILEASLAHGEKENGIPPFSNPKKYVKNEDYINGYSEYLVDVKEHIQICDSTKVEKVDSGDPKITQLNFINFQPGSIITIRVSLHANIKPALESINDLISTLTSPNNSELRAIVSRMNFADLNKALYRCDQEERDTTANKFGVYDIPGYGPLVYAGLQGVISLLADIRSNNDLGHPLCGNLRQGNWLIDYTWQRLKEDEGTAALGEWLEQACEPFKLIPRYLVPSYFDVIIVNVYSTLLDQCFNLMSSFVKDGTDFTRLLSLVSVQVGGIIKSAQLPDLSPNLDPPKPSIREVDGKSEQECLTMSAGLPHFTVGYMRNWGRDTFIALRGLFLLTGRHAEARFIILGYAATLRHGLIPNLLDRGVNARFNCRDAVWWWLYTIQCYVKEVPNGLKLLSDKVSRLFPTDDSDALPAGEVDETLSDVMQEALTVHFQGLCFRERNAGKRIDEHMTDRGFNNQIGVHPETGFVFGGNEANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIIGLCHSTLNFLAELYKQNLFPYGSVQRKNRDGSTITWSYKQWADKIKESFDTYFYVNEKPTDGELRPDLIHRRGIYKDTHGASQPWADYQLRPNFPVAMVASPNLFKPEHAWLALKKAEEILLGPLGMKTLDPADWAYDGFYNNANDSNDPKVAHGWNYHQGPEWLWPIGYFLRARLHYAPLVGGEEELKRTIASTEAIISRHLTEASNNHWRGLPELTNREGEYCADSCRTQAWSASAIVEVLHDLDKLKKDLKFDDEQVLTN

Target ORF information:

RefSeq Version	XM_008219354.2
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008219354.2

ORF Insert Sequence:

ATGGTTGCTC TGCAGAATCT AGTGTGTGAC TCCACACGAC AATTATTCAA GAAGATAGGG 
TATTTCTTTC ACCGCTGCTT GACCTTATTT TGGTCTTATC CCAAAAAGAA GACCATGGAA 
AATCTCGGCT TCTCTGCGGA TACCCAGAAC GACGACCAAG TGCGAGTACT GACTCTGAAT 
AAGGGAGAGC ACCAGGATGG GACCCTGTAC AAGCTTAAAA AAGGATGGGT CGTGGAGTTC 
AGATTAGGGG CATCGCTTCT GGGCCACAAT CTCAACATAT TCACTAATCA TCCTCTACTC 
GTCGGCAACC CGTTCGAGAG GCGCACGTAC TATCAGCTGC CATGGAATCA CGAGTCAGCT 
TTCATACGCG TCTCGCTGGC CGGCTCTTTT CATTTTTATC TGGCAGAAGC GAGTGGAGAG 
GACGCCGGAA AAGTGATAGC TTCCGGCTAC TTGTTGGTCG ATCCGGAGCT AACCGTGGGC 
GTCAACAACC AGCCGCTGCC GCTAGACTGC ATACAATGCC AGACGGTCCT CTCAAAGCTC 
ATGGGACCCT TCTCAACGTG GGAGGAGAAG CTCAGGGTCT CTCGCAAGTC AGGCTACAAC 
ATGATCCACT TCACACCGAT ACAGGCGATA GGAGACTCAA AATCAGCTTA CAGCCTCAGC 
GATCAGCTGA AGCTCAACCC CTCGTTCAAT GATGCACCGA ACAAAGAGAC CAGCTTCGAC 
GACGTCGAGA AGTTGATTAC AAAAATGAGA GAAGAGTGGA ACACTGTGAG CATTTGCGAC 
ATAGTTTTGA ATCACACGGC GAACGAGAGT CCATTTTTGA TAAACAATCC CGAATGTAGT 
TACAACTGCA ACAACTGTCC ACATCTCCGT CCGTCTTATC TTTTGGATGC CATTTTATTC 
GAGTTGACAA TACGAGTGTC GTCCGGAGAA TGGGAGCACA AAGGAATTCC TCCCGTCGTC 
GAGACTGAAG AGCACTTGAA CGCAATCCGT CATGCTCTGC ACACTTACTT TCTACCTCTG 
GCGAAAATTC ACGAACTGTA TACTGTAGAT ATCAACGAGA TCGTGTCCGA GTTTTTGAAC 
ATTGCTCGCA ACCAGATACC GCAAGACGAG CCACCCGAAA CCCATAACGA AAGCATCGTT 
ATCATAAGGG ATCCAGAATA TCGCCGCTTA AAGTCCACTA TAGATATGCA GCTTGCTTTG 
AAGATCTACA ATATTTATAG ACCCGATTGT TTTGACGAGG ACTCACGACT GAAACGCTGC 
GCGGAAGAAC TGAGAAACAA GTTGCAGGAG TTAAACGATG CCGCAACTAA CGAAATCCAA 
AATCATCTCA ATGCCGCGGT TGAAAACTCG GTAGCCGGCA TTCGATACTT CAGAGTCCAA 
GCAGATGGGC CAAGACAGAA AGAGATAAGC GAAAGAAATC CACTTGTTCC TAGGTATTTC 
ACGGATTACG GAAGTCCTAG ATCGTTTGTG GAAAGAGAAG ACATCATGTA CTCGAATTCT 
GGATGTTACT TGATGGCTCA CAACGGCTGG GTCATGAACA GCGATCCCTT GAAAAACTTT 
GCCGATGCTG ACTCTCATGT TTACATAAGA AGGGAATTGA TTGCGTGGGG CGATAGTGTC 
AAATTAAGAT ATGGCGAAAA ACCGGAAGAT TGCCCTGCTC TTTGGGAACG TATGACGACA 
TATGTTCAGC AAATGGCTAA AATCTTCGAC GGCGTCAGAC TCGACAATTG TCACTCGACC 
CCTATTCCAG TAGCAGAACA CATGTTGGAT GCTGCTCGTC AAGTAAGACC AGATTTGTAC 
GTTGCCGCTG AACTCTTCAC CAACTCCGAT CAGAAAGATA ATATTTTCGT CAATCGTCTT 
GGAATCACAT CGTTGATAAG AGAGGCCATG TCAGCGTGGG ACAGCCATGA GGAAGGCCGT 
CTGGTATACC GCTATGGTGG CGAGCCCGTA GGTGCGTTTA TCCAGGCTCG ACATCGTCCT 
CTGGCACCGA GTATCGCGCA TGCTTTATTC CTTGATCAGA CGCATGACAA CCCGAGCCCC 
ATCGAGAAGC GTAGCGTATT TGACTTATTG CCCAGTGCCG CACTGGTCTC CATGGCCTGC 
TGTGCTAGTG GCAGTAATCG CGGTTACGAC GAGCTCGTGC CTCATCATAT TCACGTTGTC 
GATGAAGACA GACAATATAC TTCTTGGACA GATAAGGAAG GACTGGCAAA GGAAAATGCA 
AAGTATGTTA GCAAAAAATC GGGAATCATT TCTGCTAAAA AGGCTCTCAA TGATCTCCAT 
TTTTGGCTGG GACAGAACTC CTTTTCTCAA GTTTTTGTCG ATCAAATGGA TGTTGATGTG 
GTAGCGGTTA CAAGACATTC ACCGACCACT CACGAGAGCG TCGTACTCGT GGCTTTTACG 
GCATTCAAGC ACCCGGATCC CAATGCTAGC GATTTGAGAA GACATGTTAG ACCTTTGAGG 
GTAGAAGGCG TAGTAGATGA AATTATTCTT GAAGCATCCC TCGCGCACGG AGAAAAAGAA 
AATGGTATCC CTCCGTTCTC TAATCCGAAG AAATACGTAA AGAACGAAGA CTACATAAAC 
GGTTACTCTG AGTACTTGGT GGACGTCAAG GAGCACATTC AAATCTGTGA TTCTACAAAA 
GTTGAAAAGG TCGATTCCGG AGATCCTAAA ATAACGCAAT TGAACTTTAT TAATTTCCAG 
CCCGGATCAA TAATCACTAT TCGGGTGTCA CTCCACGCAA ACATAAAGCC AGCTCTTGAA 
TCCATCAATG ACCTTATCTC GACCCTCACA TCTCCGAACA ATTCGGAATT GCGTGCAATT 
GTTTCGCGCA TGAACTTTGC GGACCTTAAC AAAGCACTTT ATAGGTGTGA TCAAGAAGAG 
CGAGATACGA CTGCCAATAA ATTTGGAGTT TATGATATTC CCGGTTATGG CCCACTAGTT 
TACGCTGGTT TGCAAGGTGT AATTTCATTG CTTGCTGATA TCAGATCGAA CAACGATCTT 
GGTCATCCAC TATGTGGCAA CCTTCGTCAA GGCAATTGGT TGATAGATTA TACTTGGCAA 
CGTTTGAAGG AAGATGAAGG TACTGCAGCT CTTGGAGAGT GGCTGGAGCA GGCCTGCGAA 
CCATTCAAGC TAATTCCTCG TTATTTAGTG CCCAGTTACT TTGACGTGAT CATCGTTAAC 
GTGTATTCTA CTCTCTTAGA TCAATGCTTT AATCTCATGT CTAGCTTTGT GAAAGATGGA 
ACCGATTTCA CCAGACTGCT GTCACTAGTA TCTGTGCAAG TTGGCGGTAT AATAAAATCA 
GCGCAATTGC CAGATTTATC ACCGAACCTA GATCCACCGA AACCTAGCAT ACGAGAGGTG 
GATGGCAAAT CAGAACAGGA ATGTTTGACC ATGTCTGCTG GACTACCACA CTTTACAGTA 
GGATACATGA GAAACTGGGG ACGAGATACG TTTATCGCTT TGAGAGGTCT GTTCTTATTA 
ACCGGCAGAC ATGCGGAGGC TCGATTCATC ATTCTGGGAT ACGCCGCTAC GCTTCGTCAC 
GGTTTGATAC CAAATCTCTT GGACAGAGGC GTCAACGCCA GATTCAATTG CCGAGATGCC 
GTTTGGTGGT GGTTGTACAC TATTCAATGC TATGTCAAAG AAGTCCCAAA TGGCTTAAAA 
CTTCTGTCGG ACAAAGTATC TAGATTGTTC CCTACTGACG ATTCGGACGC TCTTCCAGCC 
GGAGAAGTGG ATGAAACACT GTCGGATGTC ATGCAGGAAG CACTGACAGT TCACTTTCAA 
GGTCTGTGCT TCCGTGAGCG CAACGCCGGT AAGCGCATCG ACGAGCACAT GACTGATCGT 
GGATTCAACA ATCAGATAGG TGTGCATCCA GAGACGGGCT TTGTATTTGG CGGTAACGAG 
GCCAATTGCG GCACTTGGAT GGACAAGATG GGCTCCTCGG AAAAGGCAGG AAACAAAGGC 
AAACCTGCCA CTCCCAGAGA CGGATCTGCG GTTGAAATTA TTGGGCTCTG TCACAGCACT 
CTCAATTTTC TGGCAGAATT GTACAAACAG AATCTATTTC CATATGGTAG CGTGCAGCGG 
AAAAATCGCG ATGGCTCAAC GATAACTTGG AGTTACAAAC AGTGGGCGGA TAAGATAAAG 
GAGAGTTTCG ATACTTACTT CTATGTGAAC GAGAAACCTA CCGATGGTGA ATTACGACCG 
GACTTGATTC ATCGACGAGG AATTTACAAA GATACTCACG GAGCCTCACA ACCGTGGGCA 
GATTATCAAT TGCGACCTAA CTTCCCAGTG GCTATGGTTG CTTCGCCGAA CCTATTCAAG 
CCCGAGCACG CCTGGCTGGC ATTAAAGAAG GCCGAAGAAA TCCTCCTTGG TCCCCTAGGC 
ATGAAAACCC TAGATCCCGC TGACTGGGCC TACGATGGCT TCTACAACAA TGCCAACGAC 
TCGAATGACC CGAAAGTAGC TCACGGATGG AATTATCACC AGGGACCAGA GTGGCTATGG 
CCGATCGGCT ACTTTTTGCG AGCGCGCTTG CACTACGCGC CCCTCGTTGG TGGCGAAGAG 
GAGTTAAAAC GGACTATCGC CTCGACCGAA GCTATCATCT CGAGACATCT GACCGAAGCT 
TCAAACAACC ACTGGCGTGG ACTTCCCGAG TTAACCAATC GCGAGGGCGA GTATTGCGCT 
GATAGCTGTC GCACTCAGGC CTGGAGCGCT TCCGCAATTG TCGAGGTCCT CCACGATTTG 
GACAAGCTGA AAAAGGATCT GAAGTTTGAC GACGAGCAGG TCCTCACGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100117168 ( XM_008219355.2 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_008217577.1 Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ONd11610
Clone ID Related Accession (Same CDS sequence)	XM_008219355.2
Accession Version	XM_008219355.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-25
Organism	Nasonia vitripennis(jewel wasp)
Product	glycogen debranching enzyme isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001815014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2016 this sequence version replaced XM_008219355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACCCA AAAAGAAGAC CATGGAAAAT CTCGGCTTCT CTGCGGATAC CCAGAACGAC 
GACCAAGTGC GAGTACTGAC TCTGAATAAG GGAGAGCACC AGGATGGGAC CCTGTACAAG 
CTTAAAAAAG GATGGGTCGT GGAGTTCAGA TTAGGGGCAT CGCTTCTGGG CCACAATCTC 
AACATATTCA CTAATCATCC TCTACTCGTC GGCAACCCGT TCGAGAGGCG CACGTACTAT 
CAGCTGCCAT GGAATCACGA GTCAGCTTTC ATACGCGTCT CGCTGGCCGG CTCTTTTCAT 
TTTTATCTGG CAGAAGCGAG TGGAGAGGAC GCCGGAAAAG TGATAGCTTC CGGCTACTTG 
TTGGTCGATC CGGAGCTAAC CGTGGGCGTC AACAACCAGC CGCTGCCGCT AGACTGCATA 
CAATGCCAGA CGGTCCTCTC AAAGCTCATG GGACCCTTCT CAACGTGGGA GGAGAAGCTC 
AGGGTCTCTC GCAAGTCAGG CTACAACATG ATCCACTTCA CACCGATACA GGCGATAGGA 
GACTCAAAAT CAGCTTACAG CCTCAGCGAT CAGCTGAAGC TCAACCCCTC GTTCAATGAT 
GCACCGAACA AAGAGACCAG CTTCGACGAC GTCGAGAAGT TGATTACAAA AATGAGAGAA 
GAGTGGAACA CTGTGAGCAT TTGCGACATA GTTTTGAATC ACACGGCGAA CGAGAGTCCA 
TTTTTGATAA ACAATCCCGA ATGTAGTTAC AACTGCAACA ACTGTCCACA TCTCCGTCCG 
TCTTATCTTT TGGATGCCAT TTTATTCGAG TTGACAATAC GAGTGTCGTC CGGAGAATGG 
GAGCACAAAG GAATTCCTCC CGTCGTCGAG ACTGAAGAGC ACTTGAACGC AATCCGTCAT 
GCTCTGCACA CTTACTTTCT ACCTCTGGCG AAAATTCACG AACTGTATAC TGTAGATATC 
AACGAGATCG TGTCCGAGTT TTTGAACATT GCTCGCAACC AGATACCGCA AGACGAGCCA 
CCCGAAACCC ATAACGAAAG CATCGTTATC ATAAGGGATC CAGAATATCG CCGCTTAAAG 
TCCACTATAG ATATGCAGCT TGCTTTGAAG ATCTACAATA TTTATAGACC CGATTGTTTT 
GACGAGGACT CACGACTGAA ACGCTGCGCG GAAGAACTGA GAAACAAGTT GCAGGAGTTA 
AACGATGCCG CAACTAACGA AATCCAAAAT CATCTCAATG CCGCGGTTGA AAACTCGGTA 
GCCGGCATTC GATACTTCAG AGTCCAAGCA GATGGGCCAA GACAGAAAGA GATAAGCGAA 
AGAAATCCAC TTGTTCCTAG GTATTTCACG GATTACGGAA GTCCTAGATC GTTTGTGGAA 
AGAGAAGACA TCATGTACTC GAATTCTGGA TGTTACTTGA TGGCTCACAA CGGCTGGGTC 
ATGAACAGCG ATCCCTTGAA AAACTTTGCC GATGCTGACT CTCATGTTTA CATAAGAAGG 
GAATTGATTG CGTGGGGCGA TAGTGTCAAA TTAAGATATG GCGAAAAACC GGAAGATTGC 
CCTGCTCTTT GGGAACGTAT GACGACATAT GTTCAGCAAA TGGCTAAAAT CTTCGACGGC 
GTCAGACTCG ACAATTGTCA CTCGACCCCT ATTCCAGTAG CAGAACACAT GTTGGATGCT 
GCTCGTCAAG TAAGACCAGA TTTGTACGTT GCCGCTGAAC TCTTCACCAA CTCCGATCAG 
AAAGATAATA TTTTCGTCAA TCGTCTTGGA ATCACATCGT TGATAAGAGA GGCCATGTCA 
GCGTGGGACA GCCATGAGGA AGGCCGTCTG GTATACCGCT ATGGTGGCGA GCCCGTAGGT 
GCGTTTATCC AGGCTCGACA TCGTCCTCTG GCACCGAGTA TCGCGCATGC TTTATTCCTT 
GATCAGACGC ATGACAACCC GAGCCCCATC GAGAAGCGTA GCGTATTTGA CTTATTGCCC 
AGTGCCGCAC TGGTCTCCAT GGCCTGCTGT GCTAGTGGCA GTAATCGCGG TTACGACGAG 
CTCGTGCCTC ATCATATTCA CGTTGTCGAT GAAGACAGAC AATATACTTC TTGGACAGAT 
AAGGAAGGAC TGGCAAAGGA AAATGCAAAG TATGTTAGCA AAAAATCGGG AATCATTTCT 
GCTAAAAAGG CTCTCAATGA TCTCCATTTT TGGCTGGGAC AGAACTCCTT TTCTCAAGTT 
TTTGTCGATC AAATGGATGT TGATGTGGTA GCGGTTACAA GACATTCACC GACCACTCAC 
GAGAGCGTCG TACTCGTGGC TTTTACGGCA TTCAAGCACC CGGATCCCAA TGCTAGCGAT 
TTGAGAAGAC ATGTTAGACC TTTGAGGGTA GAAGGCGTAG TAGATGAAAT TATTCTTGAA 
GCATCCCTCG CGCACGGAGA AAAAGAAAAT GGTATCCCTC CGTTCTCTAA TCCGAAGAAA 
TACGTAAAGA ACGAAGACTA CATAAACGGT TACTCTGAGT ACTTGGTGGA CGTCAAGGAG 
CACATTCAAA TCTGTGATTC TACAAAAGTT GAAAAGGTCG ATTCCGGAGA TCCTAAAATA 
ACGCAATTGA ACTTTATTAA TTTCCAGCCC GGATCAATAA TCACTATTCG GGTGTCACTC 
CACGCAAACA TAAAGCCAGC TCTTGAATCC ATCAATGACC TTATCTCGAC CCTCACATCT 
CCGAACAATT CGGAATTGCG TGCAATTGTT TCGCGCATGA ACTTTGCGGA CCTTAACAAA 
GCACTTTATA GGTGTGATCA AGAAGAGCGA GATACGACTG CCAATAAATT TGGAGTTTAT 
GATATTCCCG GTTATGGCCC ACTAGTTTAC GCTGGTTTGC AAGGTGTAAT TTCATTGCTT 
GCTGATATCA GATCGAACAA CGATCTTGGT CATCCACTAT GTGGCAACCT TCGTCAAGGC 
AATTGGTTGA TAGATTATAC TTGGCAACGT TTGAAGGAAG ATGAAGGTAC TGCAGCTCTT 
GGAGAGTGGC TGGAGCAGGC CTGCGAACCA TTCAAGCTAA TTCCTCGTTA TTTAGTGCCC 
AGTTACTTTG ACGTGATCAT CGTTAACGTG TATTCTACTC TCTTAGATCA ATGCTTTAAT 
CTCATGTCTA GCTTTGTGAA AGATGGAACC GATTTCACCA GACTGCTGTC ACTAGTATCT 
GTGCAAGTTG GCGGTATAAT AAAATCAGCG CAATTGCCAG ATTTATCACC GAACCTAGAT 
CCACCGAAAC CTAGCATACG AGAGGTGGAT GGCAAATCAG AACAGGAATG TTTGACCATG 
TCTGCTGGAC TACCACACTT TACAGTAGGA TACATGAGAA ACTGGGGACG AGATACGTTT 
ATCGCTTTGA GAGGTCTGTT CTTATTAACC GGCAGACATG CGGAGGCTCG ATTCATCATT 
CTGGGATACG CCGCTACGCT TCGTCACGGT TTGATACCAA ATCTCTTGGA CAGAGGCGTC 
AACGCCAGAT TCAATTGCCG AGATGCCGTT TGGTGGTGGT TGTACACTAT TCAATGCTAT 
GTCAAAGAAG TCCCAAATGG CTTAAAACTT CTGTCGGACA AAGTATCTAG ATTGTTCCCT 
ACTGACGATT CGGACGCTCT TCCAGCCGGA GAAGTGGATG AAACACTGTC GGATGTCATG 
CAGGAAGCAC TGACAGTTCA CTTTCAAGGT CTGTGCTTCC GTGAGCGCAA CGCCGGTAAG 
CGCATCGACG AGCACATGAC TGATCGTGGA TTCAACAATC AGATAGGTGT GCATCCAGAG 
ACGGGCTTTG TATTTGGCGG TAACGAGGCC AATTGCGGCA CTTGGATGGA CAAGATGGGC 
TCCTCGGAAA AGGCAGGAAA CAAAGGCAAA CCTGCCACTC CCAGAGACGG ATCTGCGGTT 
GAAATTATTG GGCTCTGTCA CAGCACTCTC AATTTTCTGG CAGAATTGTA CAAACAGAAT 
CTATTTCCAT ATGGTAGCGT GCAGCGGAAA AATCGCGATG GCTCAACGAT AACTTGGAGT 
TACAAACAGT GGGCGGATAA GATAAAGGAG AGTTTCGATA CTTACTTCTA TGTGAACGAG 
AAACCTACCG ATGGTGAATT ACGACCGGAC TTGATTCATC GACGAGGAAT TTACAAAGAT 
ACTCACGGAG CCTCACAACC GTGGGCAGAT TATCAATTGC GACCTAACTT CCCAGTGGCT 
ATGGTTGCTT CGCCGAACCT ATTCAAGCCC GAGCACGCCT GGCTGGCATT AAAGAAGGCC 
GAAGAAATCC TCCTTGGTCC CCTAGGCATG AAAACCCTAG ATCCCGCTGA CTGGGCCTAC 
GATGGCTTCT ACAACAATGC CAACGACTCG AATGACCCGA AAGTAGCTCA CGGATGGAAT 
TATCACCAGG GACCAGAGTG GCTATGGCCG ATCGGCTACT TTTTGCGAGC GCGCTTGCAC 
TACGCGCCCC TCGTTGGTGG CGAAGAGGAG TTAAAACGGA CTATCGCCTC GACCGAAGCT 
ATCATCTCGA GACATCTGAC CGAAGCTTCA AACAACCACT GGCGTGGACT TCCCGAGTTA 
ACCAATCGCG AGGGCGAGTA TTGCGCTGAT AGCTGTCGCA CTCAGGCCTG GAGCGCTTCC 
GCAATTGTCG AGGTCCTCCA CGATTTGGAC AAGCTGAAAA AGGATCTGAA GTTTGACGAC 
GAGCAGGTCC TCACGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008217577.1
CDS	16..4716
Translation	MTPKKKTMENLGFSADTQNDDQVRVLTLNKGEHQDGTLYKLKKGWVVEFRLGASLLGHNLNIFTNHPLLVGNPFERRTYYQLPWNHESAFIRVSLAGSFHFYLAEASGEDAGKVIASGYLLVDPELTVGVNNQPLPLDCIQCQTVLSKLMGPFSTWEEKLRVSRKSGYNMIHFTPIQAIGDSKSAYSLSDQLKLNPSFNDAPNKETSFDDVEKLITKMREEWNTVSICDIVLNHTANESPFLINNPECSYNCNNCPHLRPSYLLDAILFELTIRVSSGEWEHKGIPPVVETEEHLNAIRHALHTYFLPLAKIHELYTVDINEIVSEFLNIARNQIPQDEPPETHNESIVIIRDPEYRRLKSTIDMQLALKIYNIYRPDCFDEDSRLKRCAEELRNKLQELNDAATNEIQNHLNAAVENSVAGIRYFRVQADGPRQKEISERNPLVPRYFTDYGSPRSFVEREDIMYSNSGCYLMAHNGWVMNSDPLKNFADADSHVYIRRELIAWGDSVKLRYGEKPEDCPALWERMTTYVQQMAKIFDGVRLDNCHSTPIPVAEHMLDAARQVRPDLYVAAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFIQARHRPLAPSIAHALFLDQTHDNPSPIEKRSVFDLLPSAALVSMACCASGSNRGYDELVPHHIHVVDEDRQYTSWTDKEGLAKENAKYVSKKSGIISAKKALNDLHFWLGQNSFSQVFVDQMDVDVVAVTRHSPTTHESVVLVAFTAFKHPDPNASDLRRHVRPLRVEGVVDEIILEASLAHGEKENGIPPFSNPKKYVKNEDYINGYSEYLVDVKEHIQICDSTKVEKVDSGDPKITQLNFINFQPGSIITIRVSLHANIKPALESINDLISTLTSPNNSELRAIVSRMNFADLNKALYRCDQEERDTTANKFGVYDIPGYGPLVYAGLQGVISLLADIRSNNDLGHPLCGNLRQGNWLIDYTWQRLKEDEGTAALGEWLEQACEPFKLIPRYLVPSYFDVIIVNVYSTLLDQCFNLMSSFVKDGTDFTRLLSLVSVQVGGIIKSAQLPDLSPNLDPPKPSIREVDGKSEQECLTMSAGLPHFTVGYMRNWGRDTFIALRGLFLLTGRHAEARFIILGYAATLRHGLIPNLLDRGVNARFNCRDAVWWWLYTIQCYVKEVPNGLKLLSDKVSRLFPTDDSDALPAGEVDETLSDVMQEALTVHFQGLCFRERNAGKRIDEHMTDRGFNNQIGVHPETGFVFGGNEANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIIGLCHSTLNFLAELYKQNLFPYGSVQRKNRDGSTITWSYKQWADKIKESFDTYFYVNEKPTDGELRPDLIHRRGIYKDTHGASQPWADYQLRPNFPVAMVASPNLFKPEHAWLALKKAEEILLGPLGMKTLDPADWAYDGFYNNANDSNDPKVAHGWNYHQGPEWLWPIGYFLRARLHYAPLVGGEEELKRTIASTEAIISRHLTEASNNHWRGLPELTNREGEYCADSCRTQAWSASAIVEVLHDLDKLKKDLKFDDEQVLTN

Target ORF information:

RefSeq Version	XM_008219355.2
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008219355.2

ORF Insert Sequence:

ATGACACCCA AAAAGAAGAC CATGGAAAAT CTCGGCTTCT CTGCGGATAC CCAGAACGAC 
GACCAAGTGC GAGTACTGAC TCTGAATAAG GGAGAGCACC AGGATGGGAC CCTGTACAAG 
CTTAAAAAAG GATGGGTCGT GGAGTTCAGA TTAGGGGCAT CGCTTCTGGG CCACAATCTC 
AACATATTCA CTAATCATCC TCTACTCGTC GGCAACCCGT TCGAGAGGCG CACGTACTAT 
CAGCTGCCAT GGAATCACGA GTCAGCTTTC ATACGCGTCT CGCTGGCCGG CTCTTTTCAT 
TTTTATCTGG CAGAAGCGAG TGGAGAGGAC GCCGGAAAAG TGATAGCTTC CGGCTACTTG 
TTGGTCGATC CGGAGCTAAC CGTGGGCGTC AACAACCAGC CGCTGCCGCT AGACTGCATA 
CAATGCCAGA CGGTCCTCTC AAAGCTCATG GGACCCTTCT CAACGTGGGA GGAGAAGCTC 
AGGGTCTCTC GCAAGTCAGG CTACAACATG ATCCACTTCA CACCGATACA GGCGATAGGA 
GACTCAAAAT CAGCTTACAG CCTCAGCGAT CAGCTGAAGC TCAACCCCTC GTTCAATGAT 
GCACCGAACA AAGAGACCAG CTTCGACGAC GTCGAGAAGT TGATTACAAA AATGAGAGAA 
GAGTGGAACA CTGTGAGCAT TTGCGACATA GTTTTGAATC ACACGGCGAA CGAGAGTCCA 
TTTTTGATAA ACAATCCCGA ATGTAGTTAC AACTGCAACA ACTGTCCACA TCTCCGTCCG 
TCTTATCTTT TGGATGCCAT TTTATTCGAG TTGACAATAC GAGTGTCGTC CGGAGAATGG 
GAGCACAAAG GAATTCCTCC CGTCGTCGAG ACTGAAGAGC ACTTGAACGC AATCCGTCAT 
GCTCTGCACA CTTACTTTCT ACCTCTGGCG AAAATTCACG AACTGTATAC TGTAGATATC 
AACGAGATCG TGTCCGAGTT TTTGAACATT GCTCGCAACC AGATACCGCA AGACGAGCCA 
CCCGAAACCC ATAACGAAAG CATCGTTATC ATAAGGGATC CAGAATATCG CCGCTTAAAG 
TCCACTATAG ATATGCAGCT TGCTTTGAAG ATCTACAATA TTTATAGACC CGATTGTTTT 
GACGAGGACT CACGACTGAA ACGCTGCGCG GAAGAACTGA GAAACAAGTT GCAGGAGTTA 
AACGATGCCG CAACTAACGA AATCCAAAAT CATCTCAATG CCGCGGTTGA AAACTCGGTA 
GCCGGCATTC GATACTTCAG AGTCCAAGCA GATGGGCCAA GACAGAAAGA GATAAGCGAA 
AGAAATCCAC TTGTTCCTAG GTATTTCACG GATTACGGAA GTCCTAGATC GTTTGTGGAA 
AGAGAAGACA TCATGTACTC GAATTCTGGA TGTTACTTGA TGGCTCACAA CGGCTGGGTC 
ATGAACAGCG ATCCCTTGAA AAACTTTGCC GATGCTGACT CTCATGTTTA CATAAGAAGG 
GAATTGATTG CGTGGGGCGA TAGTGTCAAA TTAAGATATG GCGAAAAACC GGAAGATTGC 
CCTGCTCTTT GGGAACGTAT GACGACATAT GTTCAGCAAA TGGCTAAAAT CTTCGACGGC 
GTCAGACTCG ACAATTGTCA CTCGACCCCT ATTCCAGTAG CAGAACACAT GTTGGATGCT 
GCTCGTCAAG TAAGACCAGA TTTGTACGTT GCCGCTGAAC TCTTCACCAA CTCCGATCAG 
AAAGATAATA TTTTCGTCAA TCGTCTTGGA ATCACATCGT TGATAAGAGA GGCCATGTCA 
GCGTGGGACA GCCATGAGGA AGGCCGTCTG GTATACCGCT ATGGTGGCGA GCCCGTAGGT 
GCGTTTATCC AGGCTCGACA TCGTCCTCTG GCACCGAGTA TCGCGCATGC TTTATTCCTT 
GATCAGACGC ATGACAACCC GAGCCCCATC GAGAAGCGTA GCGTATTTGA CTTATTGCCC 
AGTGCCGCAC TGGTCTCCAT GGCCTGCTGT GCTAGTGGCA GTAATCGCGG TTACGACGAG 
CTCGTGCCTC ATCATATTCA CGTTGTCGAT GAAGACAGAC AATATACTTC TTGGACAGAT 
AAGGAAGGAC TGGCAAAGGA AAATGCAAAG TATGTTAGCA AAAAATCGGG AATCATTTCT 
GCTAAAAAGG CTCTCAATGA TCTCCATTTT TGGCTGGGAC AGAACTCCTT TTCTCAAGTT 
TTTGTCGATC AAATGGATGT TGATGTGGTA GCGGTTACAA GACATTCACC GACCACTCAC 
GAGAGCGTCG TACTCGTGGC TTTTACGGCA TTCAAGCACC CGGATCCCAA TGCTAGCGAT 
TTGAGAAGAC ATGTTAGACC TTTGAGGGTA GAAGGCGTAG TAGATGAAAT TATTCTTGAA 
GCATCCCTCG CGCACGGAGA AAAAGAAAAT GGTATCCCTC CGTTCTCTAA TCCGAAGAAA 
TACGTAAAGA ACGAAGACTA CATAAACGGT TACTCTGAGT ACTTGGTGGA CGTCAAGGAG 
CACATTCAAA TCTGTGATTC TACAAAAGTT GAAAAGGTCG ATTCCGGAGA TCCTAAAATA 
ACGCAATTGA ACTTTATTAA TTTCCAGCCC GGATCAATAA TCACTATTCG GGTGTCACTC 
CACGCAAACA TAAAGCCAGC TCTTGAATCC ATCAATGACC TTATCTCGAC CCTCACATCT 
CCGAACAATT CGGAATTGCG TGCAATTGTT TCGCGCATGA ACTTTGCGGA CCTTAACAAA 
GCACTTTATA GGTGTGATCA AGAAGAGCGA GATACGACTG CCAATAAATT TGGAGTTTAT 
GATATTCCCG GTTATGGCCC ACTAGTTTAC GCTGGTTTGC AAGGTGTAAT TTCATTGCTT 
GCTGATATCA GATCGAACAA CGATCTTGGT CATCCACTAT GTGGCAACCT TCGTCAAGGC 
AATTGGTTGA TAGATTATAC TTGGCAACGT TTGAAGGAAG ATGAAGGTAC TGCAGCTCTT 
GGAGAGTGGC TGGAGCAGGC CTGCGAACCA TTCAAGCTAA TTCCTCGTTA TTTAGTGCCC 
AGTTACTTTG ACGTGATCAT CGTTAACGTG TATTCTACTC TCTTAGATCA ATGCTTTAAT 
CTCATGTCTA GCTTTGTGAA AGATGGAACC GATTTCACCA GACTGCTGTC ACTAGTATCT 
GTGCAAGTTG GCGGTATAAT AAAATCAGCG CAATTGCCAG ATTTATCACC GAACCTAGAT 
CCACCGAAAC CTAGCATACG AGAGGTGGAT GGCAAATCAG AACAGGAATG TTTGACCATG 
TCTGCTGGAC TACCACACTT TACAGTAGGA TACATGAGAA ACTGGGGACG AGATACGTTT 
ATCGCTTTGA GAGGTCTGTT CTTATTAACC GGCAGACATG CGGAGGCTCG ATTCATCATT 
CTGGGATACG CCGCTACGCT TCGTCACGGT TTGATACCAA ATCTCTTGGA CAGAGGCGTC 
AACGCCAGAT TCAATTGCCG AGATGCCGTT TGGTGGTGGT TGTACACTAT TCAATGCTAT 
GTCAAAGAAG TCCCAAATGG CTTAAAACTT CTGTCGGACA AAGTATCTAG ATTGTTCCCT 
ACTGACGATT CGGACGCTCT TCCAGCCGGA GAAGTGGATG AAACACTGTC GGATGTCATG 
CAGGAAGCAC TGACAGTTCA CTTTCAAGGT CTGTGCTTCC GTGAGCGCAA CGCCGGTAAG 
CGCATCGACG AGCACATGAC TGATCGTGGA TTCAACAATC AGATAGGTGT GCATCCAGAG 
ACGGGCTTTG TATTTGGCGG TAACGAGGCC AATTGCGGCA CTTGGATGGA CAAGATGGGC 
TCCTCGGAAA AGGCAGGAAA CAAAGGCAAA CCTGCCACTC CCAGAGACGG ATCTGCGGTT 
GAAATTATTG GGCTCTGTCA CAGCACTCTC AATTTTCTGG CAGAATTGTA CAAACAGAAT 
CTATTTCCAT ATGGTAGCGT GCAGCGGAAA AATCGCGATG GCTCAACGAT AACTTGGAGT 
TACAAACAGT GGGCGGATAA GATAAAGGAG AGTTTCGATA CTTACTTCTA TGTGAACGAG 
AAACCTACCG ATGGTGAATT ACGACCGGAC TTGATTCATC GACGAGGAAT TTACAAAGAT 
ACTCACGGAG CCTCACAACC GTGGGCAGAT TATCAATTGC GACCTAACTT CCCAGTGGCT 
ATGGTTGCTT CGCCGAACCT ATTCAAGCCC GAGCACGCCT GGCTGGCATT AAAGAAGGCC 
GAAGAAATCC TCCTTGGTCC CCTAGGCATG AAAACCCTAG ATCCCGCTGA CTGGGCCTAC 
GATGGCTTCT ACAACAATGC CAACGACTCG AATGACCCGA AAGTAGCTCA CGGATGGAAT 
TATCACCAGG GACCAGAGTG GCTATGGCCG ATCGGCTACT TTTTGCGAGC GCGCTTGCAC 
TACGCGCCCC TCGTTGGTGG CGAAGAGGAG TTAAAACGGA CTATCGCCTC GACCGAAGCT 
ATCATCTCGA GACATCTGAC CGAAGCTTCA AACAACCACT GGCGTGGACT TCCCGAGTTA 
ACCAATCGCG AGGGCGAGTA TTGCGCTGAT AGCTGTCGCA CTCAGGCCTG GAGCGCTTCC 
GCAATTGTCG AGGTCCTCCA CGATTTGGAC AAGCTGAAAA AGGATCTGAA GTTTGACGAC 
GAGCAGGTCC TCACGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100117168 ( XM_008219356.2 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_008217578.1 Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ONd11611
Clone ID Related Accession (Same CDS sequence)	XM_008219356.2 , XM_008219358.2 , XM_016986671.1 , XM_008219357.2 , XM_016986672.1
Accession Version	XM_008219356.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-25
Organism	Nasonia vitripennis(jewel wasp)
Product	glycogen debranching enzyme isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001815014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2016 this sequence version replaced XM_008219356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATC TCGGCTTCTC TGCGGATACC CAGAACGACG ACCAAGTGCG AGTACTGACT 
CTGAATAAGG GAGAGCACCA GGATGGGACC CTGTACAAGC TTAAAAAAGG ATGGGTCGTG 
GAGTTCAGAT TAGGGGCATC GCTTCTGGGC CACAATCTCA ACATATTCAC TAATCATCCT 
CTACTCGTCG GCAACCCGTT CGAGAGGCGC ACGTACTATC AGCTGCCATG GAATCACGAG 
TCAGCTTTCA TACGCGTCTC GCTGGCCGGC TCTTTTCATT TTTATCTGGC AGAAGCGAGT 
GGAGAGGACG CCGGAAAAGT GATAGCTTCC GGCTACTTGT TGGTCGATCC GGAGCTAACC 
GTGGGCGTCA ACAACCAGCC GCTGCCGCTA GACTGCATAC AATGCCAGAC GGTCCTCTCA 
AAGCTCATGG GACCCTTCTC AACGTGGGAG GAGAAGCTCA GGGTCTCTCG CAAGTCAGGC 
TACAACATGA TCCACTTCAC ACCGATACAG GCGATAGGAG ACTCAAAATC AGCTTACAGC 
CTCAGCGATC AGCTGAAGCT CAACCCCTCG TTCAATGATG CACCGAACAA AGAGACCAGC 
TTCGACGACG TCGAGAAGTT GATTACAAAA ATGAGAGAAG AGTGGAACAC TGTGAGCATT 
TGCGACATAG TTTTGAATCA CACGGCGAAC GAGAGTCCAT TTTTGATAAA CAATCCCGAA 
TGTAGTTACA ACTGCAACAA CTGTCCACAT CTCCGTCCGT CTTATCTTTT GGATGCCATT 
TTATTCGAGT TGACAATACG AGTGTCGTCC GGAGAATGGG AGCACAAAGG AATTCCTCCC 
GTCGTCGAGA CTGAAGAGCA CTTGAACGCA ATCCGTCATG CTCTGCACAC TTACTTTCTA 
CCTCTGGCGA AAATTCACGA ACTGTATACT GTAGATATCA ACGAGATCGT GTCCGAGTTT 
TTGAACATTG CTCGCAACCA GATACCGCAA GACGAGCCAC CCGAAACCCA TAACGAAAGC 
ATCGTTATCA TAAGGGATCC AGAATATCGC CGCTTAAAGT CCACTATAGA TATGCAGCTT 
GCTTTGAAGA TCTACAATAT TTATAGACCC GATTGTTTTG ACGAGGACTC ACGACTGAAA 
CGCTGCGCGG AAGAACTGAG AAACAAGTTG CAGGAGTTAA ACGATGCCGC AACTAACGAA 
ATCCAAAATC ATCTCAATGC CGCGGTTGAA AACTCGGTAG CCGGCATTCG ATACTTCAGA 
GTCCAAGCAG ATGGGCCAAG ACAGAAAGAG ATAAGCGAAA GAAATCCACT TGTTCCTAGG 
TATTTCACGG ATTACGGAAG TCCTAGATCG TTTGTGGAAA GAGAAGACAT CATGTACTCG 
AATTCTGGAT GTTACTTGAT GGCTCACAAC GGCTGGGTCA TGAACAGCGA TCCCTTGAAA 
AACTTTGCCG ATGCTGACTC TCATGTTTAC ATAAGAAGGG AATTGATTGC GTGGGGCGAT 
AGTGTCAAAT TAAGATATGG CGAAAAACCG GAAGATTGCC CTGCTCTTTG GGAACGTATG 
ACGACATATG TTCAGCAAAT GGCTAAAATC TTCGACGGCG TCAGACTCGA CAATTGTCAC 
TCGACCCCTA TTCCAGTAGC AGAACACATG TTGGATGCTG CTCGTCAAGT AAGACCAGAT 
TTGTACGTTG CCGCTGAACT CTTCACCAAC TCCGATCAGA AAGATAATAT TTTCGTCAAT 
CGTCTTGGAA TCACATCGTT GATAAGAGAG GCCATGTCAG CGTGGGACAG CCATGAGGAA 
GGCCGTCTGG TATACCGCTA TGGTGGCGAG CCCGTAGGTG CGTTTATCCA GGCTCGACAT 
CGTCCTCTGG CACCGAGTAT CGCGCATGCT TTATTCCTTG ATCAGACGCA TGACAACCCG 
AGCCCCATCG AGAAGCGTAG CGTATTTGAC TTATTGCCCA GTGCCGCACT GGTCTCCATG 
GCCTGCTGTG CTAGTGGCAG TAATCGCGGT TACGACGAGC TCGTGCCTCA TCATATTCAC 
GTTGTCGATG AAGACAGACA ATATACTTCT TGGACAGATA AGGAAGGACT GGCAAAGGAA 
AATGCAAAGT ATGTTAGCAA AAAATCGGGA ATCATTTCTG CTAAAAAGGC TCTCAATGAT 
CTCCATTTTT GGCTGGGACA GAACTCCTTT TCTCAAGTTT TTGTCGATCA AATGGATGTT 
GATGTGGTAG CGGTTACAAG ACATTCACCG ACCACTCACG AGAGCGTCGT ACTCGTGGCT 
TTTACGGCAT TCAAGCACCC GGATCCCAAT GCTAGCGATT TGAGAAGACA TGTTAGACCT 
TTGAGGGTAG AAGGCGTAGT AGATGAAATT ATTCTTGAAG CATCCCTCGC GCACGGAGAA 
AAAGAAAATG GTATCCCTCC GTTCTCTAAT CCGAAGAAAT ACGTAAAGAA CGAAGACTAC 
ATAAACGGTT ACTCTGAGTA CTTGGTGGAC GTCAAGGAGC ACATTCAAAT CTGTGATTCT 
ACAAAAGTTG AAAAGGTCGA TTCCGGAGAT CCTAAAATAA CGCAATTGAA CTTTATTAAT 
TTCCAGCCCG GATCAATAAT CACTATTCGG GTGTCACTCC ACGCAAACAT AAAGCCAGCT 
CTTGAATCCA TCAATGACCT TATCTCGACC CTCACATCTC CGAACAATTC GGAATTGCGT 
GCAATTGTTT CGCGCATGAA CTTTGCGGAC CTTAACAAAG CACTTTATAG GTGTGATCAA 
GAAGAGCGAG ATACGACTGC CAATAAATTT GGAGTTTATG ATATTCCCGG TTATGGCCCA 
CTAGTTTACG CTGGTTTGCA AGGTGTAATT TCATTGCTTG CTGATATCAG ATCGAACAAC 
GATCTTGGTC ATCCACTATG TGGCAACCTT CGTCAAGGCA ATTGGTTGAT AGATTATACT 
TGGCAACGTT TGAAGGAAGA TGAAGGTACT GCAGCTCTTG GAGAGTGGCT GGAGCAGGCC 
TGCGAACCAT TCAAGCTAAT TCCTCGTTAT TTAGTGCCCA GTTACTTTGA CGTGATCATC 
GTTAACGTGT ATTCTACTCT CTTAGATCAA TGCTTTAATC TCATGTCTAG CTTTGTGAAA 
GATGGAACCG ATTTCACCAG ACTGCTGTCA CTAGTATCTG TGCAAGTTGG CGGTATAATA 
AAATCAGCGC AATTGCCAGA TTTATCACCG AACCTAGATC CACCGAAACC TAGCATACGA 
GAGGTGGATG GCAAATCAGA ACAGGAATGT TTGACCATGT CTGCTGGACT ACCACACTTT 
ACAGTAGGAT ACATGAGAAA CTGGGGACGA GATACGTTTA TCGCTTTGAG AGGTCTGTTC 
TTATTAACCG GCAGACATGC GGAGGCTCGA TTCATCATTC TGGGATACGC CGCTACGCTT 
CGTCACGGTT TGATACCAAA TCTCTTGGAC AGAGGCGTCA ACGCCAGATT CAATTGCCGA 
GATGCCGTTT GGTGGTGGTT GTACACTATT CAATGCTATG TCAAAGAAGT CCCAAATGGC 
TTAAAACTTC TGTCGGACAA AGTATCTAGA TTGTTCCCTA CTGACGATTC GGACGCTCTT 
CCAGCCGGAG AAGTGGATGA AACACTGTCG GATGTCATGC AGGAAGCACT GACAGTTCAC 
TTTCAAGGTC TGTGCTTCCG TGAGCGCAAC GCCGGTAAGC GCATCGACGA GCACATGACT 
GATCGTGGAT TCAACAATCA GATAGGTGTG CATCCAGAGA CGGGCTTTGT ATTTGGCGGT 
AACGAGGCCA ATTGCGGCAC TTGGATGGAC AAGATGGGCT CCTCGGAAAA GGCAGGAAAC 
AAAGGCAAAC CTGCCACTCC CAGAGACGGA TCTGCGGTTG AAATTATTGG GCTCTGTCAC 
AGCACTCTCA ATTTTCTGGC AGAATTGTAC AAACAGAATC TATTTCCATA TGGTAGCGTG 
CAGCGGAAAA ATCGCGATGG CTCAACGATA ACTTGGAGTT ACAAACAGTG GGCGGATAAG 
ATAAAGGAGA GTTTCGATAC TTACTTCTAT GTGAACGAGA AACCTACCGA TGGTGAATTA 
CGACCGGACT TGATTCATCG ACGAGGAATT TACAAAGATA CTCACGGAGC CTCACAACCG 
TGGGCAGATT ATCAATTGCG ACCTAACTTC CCAGTGGCTA TGGTTGCTTC GCCGAACCTA 
TTCAAGCCCG AGCACGCCTG GCTGGCATTA AAGAAGGCCG AAGAAATCCT CCTTGGTCCC 
CTAGGCATGA AAACCCTAGA TCCCGCTGAC TGGGCCTACG ATGGCTTCTA CAACAATGCC 
AACGACTCGA ATGACCCGAA AGTAGCTCAC GGATGGAATT ATCACCAGGG ACCAGAGTGG 
CTATGGCCGA TCGGCTACTT TTTGCGAGCG CGCTTGCACT ACGCGCCCCT CGTTGGTGGC 
GAAGAGGAGT TAAAACGGAC TATCGCCTCG ACCGAAGCTA TCATCTCGAG ACATCTGACC 
GAAGCTTCAA ACAACCACTG GCGTGGACTT CCCGAGTTAA CCAATCGCGA GGGCGAGTAT 
TGCGCTGATA GCTGTCGCAC TCAGGCCTGG AGCGCTTCCG CAATTGTCGA GGTCCTCCAC 
GATTTGGACA AGCTGAAAAA GGATCTGAAG TTTGACGACG AGCAGGTCCT CACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008217578.1
CDS	525..5204
Translation	MENLGFSADTQNDDQVRVLTLNKGEHQDGTLYKLKKGWVVEFRLGASLLGHNLNIFTNHPLLVGNPFERRTYYQLPWNHESAFIRVSLAGSFHFYLAEASGEDAGKVIASGYLLVDPELTVGVNNQPLPLDCIQCQTVLSKLMGPFSTWEEKLRVSRKSGYNMIHFTPIQAIGDSKSAYSLSDQLKLNPSFNDAPNKETSFDDVEKLITKMREEWNTVSICDIVLNHTANESPFLINNPECSYNCNNCPHLRPSYLLDAILFELTIRVSSGEWEHKGIPPVVETEEHLNAIRHALHTYFLPLAKIHELYTVDINEIVSEFLNIARNQIPQDEPPETHNESIVIIRDPEYRRLKSTIDMQLALKIYNIYRPDCFDEDSRLKRCAEELRNKLQELNDAATNEIQNHLNAAVENSVAGIRYFRVQADGPRQKEISERNPLVPRYFTDYGSPRSFVEREDIMYSNSGCYLMAHNGWVMNSDPLKNFADADSHVYIRRELIAWGDSVKLRYGEKPEDCPALWERMTTYVQQMAKIFDGVRLDNCHSTPIPVAEHMLDAARQVRPDLYVAAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFIQARHRPLAPSIAHALFLDQTHDNPSPIEKRSVFDLLPSAALVSMACCASGSNRGYDELVPHHIHVVDEDRQYTSWTDKEGLAKENAKYVSKKSGIISAKKALNDLHFWLGQNSFSQVFVDQMDVDVVAVTRHSPTTHESVVLVAFTAFKHPDPNASDLRRHVRPLRVEGVVDEIILEASLAHGEKENGIPPFSNPKKYVKNEDYINGYSEYLVDVKEHIQICDSTKVEKVDSGDPKITQLNFINFQPGSIITIRVSLHANIKPALESINDLISTLTSPNNSELRAIVSRMNFADLNKALYRCDQEERDTTANKFGVYDIPGYGPLVYAGLQGVISLLADIRSNNDLGHPLCGNLRQGNWLIDYTWQRLKEDEGTAALGEWLEQACEPFKLIPRYLVPSYFDVIIVNVYSTLLDQCFNLMSSFVKDGTDFTRLLSLVSVQVGGIIKSAQLPDLSPNLDPPKPSIREVDGKSEQECLTMSAGLPHFTVGYMRNWGRDTFIALRGLFLLTGRHAEARFIILGYAATLRHGLIPNLLDRGVNARFNCRDAVWWWLYTIQCYVKEVPNGLKLLSDKVSRLFPTDDSDALPAGEVDETLSDVMQEALTVHFQGLCFRERNAGKRIDEHMTDRGFNNQIGVHPETGFVFGGNEANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIIGLCHSTLNFLAELYKQNLFPYGSVQRKNRDGSTITWSYKQWADKIKESFDTYFYVNEKPTDGELRPDLIHRRGIYKDTHGASQPWADYQLRPNFPVAMVASPNLFKPEHAWLALKKAEEILLGPLGMKTLDPADWAYDGFYNNANDSNDPKVAHGWNYHQGPEWLWPIGYFLRARLHYAPLVGGEEELKRTIASTEAIISRHLTEASNNHWRGLPELTNREGEYCADSCRTQAWSASAIVEVLHDLDKLKKDLKFDDEQVLTN

Target ORF information:

RefSeq Version	XM_008219356.2
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008219356.2

ORF Insert Sequence:

ATGGAAAATC TCGGCTTCTC TGCGGATACC CAGAACGACG ACCAAGTGCG AGTACTGACT 
CTGAATAAGG GAGAGCACCA GGATGGGACC CTGTACAAGC TTAAAAAAGG ATGGGTCGTG 
GAGTTCAGAT TAGGGGCATC GCTTCTGGGC CACAATCTCA ACATATTCAC TAATCATCCT 
CTACTCGTCG GCAACCCGTT CGAGAGGCGC ACGTACTATC AGCTGCCATG GAATCACGAG 
TCAGCTTTCA TACGCGTCTC GCTGGCCGGC TCTTTTCATT TTTATCTGGC AGAAGCGAGT 
GGAGAGGACG CCGGAAAAGT GATAGCTTCC GGCTACTTGT TGGTCGATCC GGAGCTAACC 
GTGGGCGTCA ACAACCAGCC GCTGCCGCTA GACTGCATAC AATGCCAGAC GGTCCTCTCA 
AAGCTCATGG GACCCTTCTC AACGTGGGAG GAGAAGCTCA GGGTCTCTCG CAAGTCAGGC 
TACAACATGA TCCACTTCAC ACCGATACAG GCGATAGGAG ACTCAAAATC AGCTTACAGC 
CTCAGCGATC AGCTGAAGCT CAACCCCTCG TTCAATGATG CACCGAACAA AGAGACCAGC 
TTCGACGACG TCGAGAAGTT GATTACAAAA ATGAGAGAAG AGTGGAACAC TGTGAGCATT 
TGCGACATAG TTTTGAATCA CACGGCGAAC GAGAGTCCAT TTTTGATAAA CAATCCCGAA 
TGTAGTTACA ACTGCAACAA CTGTCCACAT CTCCGTCCGT CTTATCTTTT GGATGCCATT 
TTATTCGAGT TGACAATACG AGTGTCGTCC GGAGAATGGG AGCACAAAGG AATTCCTCCC 
GTCGTCGAGA CTGAAGAGCA CTTGAACGCA ATCCGTCATG CTCTGCACAC TTACTTTCTA 
CCTCTGGCGA AAATTCACGA ACTGTATACT GTAGATATCA ACGAGATCGT GTCCGAGTTT 
TTGAACATTG CTCGCAACCA GATACCGCAA GACGAGCCAC CCGAAACCCA TAACGAAAGC 
ATCGTTATCA TAAGGGATCC AGAATATCGC CGCTTAAAGT CCACTATAGA TATGCAGCTT 
GCTTTGAAGA TCTACAATAT TTATAGACCC GATTGTTTTG ACGAGGACTC ACGACTGAAA 
CGCTGCGCGG AAGAACTGAG AAACAAGTTG CAGGAGTTAA ACGATGCCGC AACTAACGAA 
ATCCAAAATC ATCTCAATGC CGCGGTTGAA AACTCGGTAG CCGGCATTCG ATACTTCAGA 
GTCCAAGCAG ATGGGCCAAG ACAGAAAGAG ATAAGCGAAA GAAATCCACT TGTTCCTAGG 
TATTTCACGG ATTACGGAAG TCCTAGATCG TTTGTGGAAA GAGAAGACAT CATGTACTCG 
AATTCTGGAT GTTACTTGAT GGCTCACAAC GGCTGGGTCA TGAACAGCGA TCCCTTGAAA 
AACTTTGCCG ATGCTGACTC TCATGTTTAC ATAAGAAGGG AATTGATTGC GTGGGGCGAT 
AGTGTCAAAT TAAGATATGG CGAAAAACCG GAAGATTGCC CTGCTCTTTG GGAACGTATG 
ACGACATATG TTCAGCAAAT GGCTAAAATC TTCGACGGCG TCAGACTCGA CAATTGTCAC 
TCGACCCCTA TTCCAGTAGC AGAACACATG TTGGATGCTG CTCGTCAAGT AAGACCAGAT 
TTGTACGTTG CCGCTGAACT CTTCACCAAC TCCGATCAGA AAGATAATAT TTTCGTCAAT 
CGTCTTGGAA TCACATCGTT GATAAGAGAG GCCATGTCAG CGTGGGACAG CCATGAGGAA 
GGCCGTCTGG TATACCGCTA TGGTGGCGAG CCCGTAGGTG CGTTTATCCA GGCTCGACAT 
CGTCCTCTGG CACCGAGTAT CGCGCATGCT TTATTCCTTG ATCAGACGCA TGACAACCCG 
AGCCCCATCG AGAAGCGTAG CGTATTTGAC TTATTGCCCA GTGCCGCACT GGTCTCCATG 
GCCTGCTGTG CTAGTGGCAG TAATCGCGGT TACGACGAGC TCGTGCCTCA TCATATTCAC 
GTTGTCGATG AAGACAGACA ATATACTTCT TGGACAGATA AGGAAGGACT GGCAAAGGAA 
AATGCAAAGT ATGTTAGCAA AAAATCGGGA ATCATTTCTG CTAAAAAGGC TCTCAATGAT 
CTCCATTTTT GGCTGGGACA GAACTCCTTT TCTCAAGTTT TTGTCGATCA AATGGATGTT 
GATGTGGTAG CGGTTACAAG ACATTCACCG ACCACTCACG AGAGCGTCGT ACTCGTGGCT 
TTTACGGCAT TCAAGCACCC GGATCCCAAT GCTAGCGATT TGAGAAGACA TGTTAGACCT 
TTGAGGGTAG AAGGCGTAGT AGATGAAATT ATTCTTGAAG CATCCCTCGC GCACGGAGAA 
AAAGAAAATG GTATCCCTCC GTTCTCTAAT CCGAAGAAAT ACGTAAAGAA CGAAGACTAC 
ATAAACGGTT ACTCTGAGTA CTTGGTGGAC GTCAAGGAGC ACATTCAAAT CTGTGATTCT 
ACAAAAGTTG AAAAGGTCGA TTCCGGAGAT CCTAAAATAA CGCAATTGAA CTTTATTAAT 
TTCCAGCCCG GATCAATAAT CACTATTCGG GTGTCACTCC ACGCAAACAT AAAGCCAGCT 
CTTGAATCCA TCAATGACCT TATCTCGACC CTCACATCTC CGAACAATTC GGAATTGCGT 
GCAATTGTTT CGCGCATGAA CTTTGCGGAC CTTAACAAAG CACTTTATAG GTGTGATCAA 
GAAGAGCGAG ATACGACTGC CAATAAATTT GGAGTTTATG ATATTCCCGG TTATGGCCCA 
CTAGTTTACG CTGGTTTGCA AGGTGTAATT TCATTGCTTG CTGATATCAG ATCGAACAAC 
GATCTTGGTC ATCCACTATG TGGCAACCTT CGTCAAGGCA ATTGGTTGAT AGATTATACT 
TGGCAACGTT TGAAGGAAGA TGAAGGTACT GCAGCTCTTG GAGAGTGGCT GGAGCAGGCC 
TGCGAACCAT TCAAGCTAAT TCCTCGTTAT TTAGTGCCCA GTTACTTTGA CGTGATCATC 
GTTAACGTGT ATTCTACTCT CTTAGATCAA TGCTTTAATC TCATGTCTAG CTTTGTGAAA 
GATGGAACCG ATTTCACCAG ACTGCTGTCA CTAGTATCTG TGCAAGTTGG CGGTATAATA 
AAATCAGCGC AATTGCCAGA TTTATCACCG AACCTAGATC CACCGAAACC TAGCATACGA 
GAGGTGGATG GCAAATCAGA ACAGGAATGT TTGACCATGT CTGCTGGACT ACCACACTTT 
ACAGTAGGAT ACATGAGAAA CTGGGGACGA GATACGTTTA TCGCTTTGAG AGGTCTGTTC 
TTATTAACCG GCAGACATGC GGAGGCTCGA TTCATCATTC TGGGATACGC CGCTACGCTT 
CGTCACGGTT TGATACCAAA TCTCTTGGAC AGAGGCGTCA ACGCCAGATT CAATTGCCGA 
GATGCCGTTT GGTGGTGGTT GTACACTATT CAATGCTATG TCAAAGAAGT CCCAAATGGC 
TTAAAACTTC TGTCGGACAA AGTATCTAGA TTGTTCCCTA CTGACGATTC GGACGCTCTT 
CCAGCCGGAG AAGTGGATGA AACACTGTCG GATGTCATGC AGGAAGCACT GACAGTTCAC 
TTTCAAGGTC TGTGCTTCCG TGAGCGCAAC GCCGGTAAGC GCATCGACGA GCACATGACT 
GATCGTGGAT TCAACAATCA GATAGGTGTG CATCCAGAGA CGGGCTTTGT ATTTGGCGGT 
AACGAGGCCA ATTGCGGCAC TTGGATGGAC AAGATGGGCT CCTCGGAAAA GGCAGGAAAC 
AAAGGCAAAC CTGCCACTCC CAGAGACGGA TCTGCGGTTG AAATTATTGG GCTCTGTCAC 
AGCACTCTCA ATTTTCTGGC AGAATTGTAC AAACAGAATC TATTTCCATA TGGTAGCGTG 
CAGCGGAAAA ATCGCGATGG CTCAACGATA ACTTGGAGTT ACAAACAGTG GGCGGATAAG 
ATAAAGGAGA GTTTCGATAC TTACTTCTAT GTGAACGAGA AACCTACCGA TGGTGAATTA 
CGACCGGACT TGATTCATCG ACGAGGAATT TACAAAGATA CTCACGGAGC CTCACAACCG 
TGGGCAGATT ATCAATTGCG ACCTAACTTC CCAGTGGCTA TGGTTGCTTC GCCGAACCTA 
TTCAAGCCCG AGCACGCCTG GCTGGCATTA AAGAAGGCCG AAGAAATCCT CCTTGGTCCC 
CTAGGCATGA AAACCCTAGA TCCCGCTGAC TGGGCCTACG ATGGCTTCTA CAACAATGCC 
AACGACTCGA ATGACCCGAA AGTAGCTCAC GGATGGAATT ATCACCAGGG ACCAGAGTGG 
CTATGGCCGA TCGGCTACTT TTTGCGAGCG CGCTTGCACT ACGCGCCCCT CGTTGGTGGC 
GAAGAGGAGT TAAAACGGAC TATCGCCTCG ACCGAAGCTA TCATCTCGAG ACATCTGACC 
GAAGCTTCAA ACAACCACTG GCGTGGACTT CCCGAGTTAA CCAATCGCGA GGGCGAGTAT 
TGCGCTGATA GCTGTCGCAC TCAGGCCTGG AGCGCTTCCG CAATTGTCGA GGTCCTCCAC 
GATTTGGACA AGCTGAAAAA GGATCTGAAG TTTGACGACG AGCAGGTCCT CACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100117168 ( XM_008219358.2 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_008217580.1 Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X9, mRNA.

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CloneID	ONd11611
Clone ID Related Accession (Same CDS sequence)	XM_008219356.2 , XM_008219358.2 , XM_016986671.1 , XM_008219357.2 , XM_016986672.1
Accession Version	XM_008219358.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-25
Organism	Nasonia vitripennis(jewel wasp)
Product	glycogen debranching enzyme isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001815014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2016 this sequence version replaced XM_008219358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATC TCGGCTTCTC TGCGGATACC CAGAACGACG ACCAAGTGCG AGTACTGACT 
CTGAATAAGG GAGAGCACCA GGATGGGACC CTGTACAAGC TTAAAAAAGG ATGGGTCGTG 
GAGTTCAGAT TAGGGGCATC GCTTCTGGGC CACAATCTCA ACATATTCAC TAATCATCCT 
CTACTCGTCG GCAACCCGTT CGAGAGGCGC ACGTACTATC AGCTGCCATG GAATCACGAG 
TCAGCTTTCA TACGCGTCTC GCTGGCCGGC TCTTTTCATT TTTATCTGGC AGAAGCGAGT 
GGAGAGGACG CCGGAAAAGT GATAGCTTCC GGCTACTTGT TGGTCGATCC GGAGCTAACC 
GTGGGCGTCA ACAACCAGCC GCTGCCGCTA GACTGCATAC AATGCCAGAC GGTCCTCTCA 
AAGCTCATGG GACCCTTCTC AACGTGGGAG GAGAAGCTCA GGGTCTCTCG CAAGTCAGGC 
TACAACATGA TCCACTTCAC ACCGATACAG GCGATAGGAG ACTCAAAATC AGCTTACAGC 
CTCAGCGATC AGCTGAAGCT CAACCCCTCG TTCAATGATG CACCGAACAA AGAGACCAGC 
TTCGACGACG TCGAGAAGTT GATTACAAAA ATGAGAGAAG AGTGGAACAC TGTGAGCATT 
TGCGACATAG TTTTGAATCA CACGGCGAAC GAGAGTCCAT TTTTGATAAA CAATCCCGAA 
TGTAGTTACA ACTGCAACAA CTGTCCACAT CTCCGTCCGT CTTATCTTTT GGATGCCATT 
TTATTCGAGT TGACAATACG AGTGTCGTCC GGAGAATGGG AGCACAAAGG AATTCCTCCC 
GTCGTCGAGA CTGAAGAGCA CTTGAACGCA ATCCGTCATG CTCTGCACAC TTACTTTCTA 
CCTCTGGCGA AAATTCACGA ACTGTATACT GTAGATATCA ACGAGATCGT GTCCGAGTTT 
TTGAACATTG CTCGCAACCA GATACCGCAA GACGAGCCAC CCGAAACCCA TAACGAAAGC 
ATCGTTATCA TAAGGGATCC AGAATATCGC CGCTTAAAGT CCACTATAGA TATGCAGCTT 
GCTTTGAAGA TCTACAATAT TTATAGACCC GATTGTTTTG ACGAGGACTC ACGACTGAAA 
CGCTGCGCGG AAGAACTGAG AAACAAGTTG CAGGAGTTAA ACGATGCCGC AACTAACGAA 
ATCCAAAATC ATCTCAATGC CGCGGTTGAA AACTCGGTAG CCGGCATTCG ATACTTCAGA 
GTCCAAGCAG ATGGGCCAAG ACAGAAAGAG ATAAGCGAAA GAAATCCACT TGTTCCTAGG 
TATTTCACGG ATTACGGAAG TCCTAGATCG TTTGTGGAAA GAGAAGACAT CATGTACTCG 
AATTCTGGAT GTTACTTGAT GGCTCACAAC GGCTGGGTCA TGAACAGCGA TCCCTTGAAA 
AACTTTGCCG ATGCTGACTC TCATGTTTAC ATAAGAAGGG AATTGATTGC GTGGGGCGAT 
AGTGTCAAAT TAAGATATGG CGAAAAACCG GAAGATTGCC CTGCTCTTTG GGAACGTATG 
ACGACATATG TTCAGCAAAT GGCTAAAATC TTCGACGGCG TCAGACTCGA CAATTGTCAC 
TCGACCCCTA TTCCAGTAGC AGAACACATG TTGGATGCTG CTCGTCAAGT AAGACCAGAT 
TTGTACGTTG CCGCTGAACT CTTCACCAAC TCCGATCAGA AAGATAATAT TTTCGTCAAT 
CGTCTTGGAA TCACATCGTT GATAAGAGAG GCCATGTCAG CGTGGGACAG CCATGAGGAA 
GGCCGTCTGG TATACCGCTA TGGTGGCGAG CCCGTAGGTG CGTTTATCCA GGCTCGACAT 
CGTCCTCTGG CACCGAGTAT CGCGCATGCT TTATTCCTTG ATCAGACGCA TGACAACCCG 
AGCCCCATCG AGAAGCGTAG CGTATTTGAC TTATTGCCCA GTGCCGCACT GGTCTCCATG 
GCCTGCTGTG CTAGTGGCAG TAATCGCGGT TACGACGAGC TCGTGCCTCA TCATATTCAC 
GTTGTCGATG AAGACAGACA ATATACTTCT TGGACAGATA AGGAAGGACT GGCAAAGGAA 
AATGCAAAGT ATGTTAGCAA AAAATCGGGA ATCATTTCTG CTAAAAAGGC TCTCAATGAT 
CTCCATTTTT GGCTGGGACA GAACTCCTTT TCTCAAGTTT TTGTCGATCA AATGGATGTT 
GATGTGGTAG CGGTTACAAG ACATTCACCG ACCACTCACG AGAGCGTCGT ACTCGTGGCT 
TTTACGGCAT TCAAGCACCC GGATCCCAAT GCTAGCGATT TGAGAAGACA TGTTAGACCT 
TTGAGGGTAG AAGGCGTAGT AGATGAAATT ATTCTTGAAG CATCCCTCGC GCACGGAGAA 
AAAGAAAATG GTATCCCTCC GTTCTCTAAT CCGAAGAAAT ACGTAAAGAA CGAAGACTAC 
ATAAACGGTT ACTCTGAGTA CTTGGTGGAC GTCAAGGAGC ACATTCAAAT CTGTGATTCT 
ACAAAAGTTG AAAAGGTCGA TTCCGGAGAT CCTAAAATAA CGCAATTGAA CTTTATTAAT 
TTCCAGCCCG GATCAATAAT CACTATTCGG GTGTCACTCC ACGCAAACAT AAAGCCAGCT 
CTTGAATCCA TCAATGACCT TATCTCGACC CTCACATCTC CGAACAATTC GGAATTGCGT 
GCAATTGTTT CGCGCATGAA CTTTGCGGAC CTTAACAAAG CACTTTATAG GTGTGATCAA 
GAAGAGCGAG ATACGACTGC CAATAAATTT GGAGTTTATG ATATTCCCGG TTATGGCCCA 
CTAGTTTACG CTGGTTTGCA AGGTGTAATT TCATTGCTTG CTGATATCAG ATCGAACAAC 
GATCTTGGTC ATCCACTATG TGGCAACCTT CGTCAAGGCA ATTGGTTGAT AGATTATACT 
TGGCAACGTT TGAAGGAAGA TGAAGGTACT GCAGCTCTTG GAGAGTGGCT GGAGCAGGCC 
TGCGAACCAT TCAAGCTAAT TCCTCGTTAT TTAGTGCCCA GTTACTTTGA CGTGATCATC 
GTTAACGTGT ATTCTACTCT CTTAGATCAA TGCTTTAATC TCATGTCTAG CTTTGTGAAA 
GATGGAACCG ATTTCACCAG ACTGCTGTCA CTAGTATCTG TGCAAGTTGG CGGTATAATA 
AAATCAGCGC AATTGCCAGA TTTATCACCG AACCTAGATC CACCGAAACC TAGCATACGA 
GAGGTGGATG GCAAATCAGA ACAGGAATGT TTGACCATGT CTGCTGGACT ACCACACTTT 
ACAGTAGGAT ACATGAGAAA CTGGGGACGA GATACGTTTA TCGCTTTGAG AGGTCTGTTC 
TTATTAACCG GCAGACATGC GGAGGCTCGA TTCATCATTC TGGGATACGC CGCTACGCTT 
CGTCACGGTT TGATACCAAA TCTCTTGGAC AGAGGCGTCA ACGCCAGATT CAATTGCCGA 
GATGCCGTTT GGTGGTGGTT GTACACTATT CAATGCTATG TCAAAGAAGT CCCAAATGGC 
TTAAAACTTC TGTCGGACAA AGTATCTAGA TTGTTCCCTA CTGACGATTC GGACGCTCTT 
CCAGCCGGAG AAGTGGATGA AACACTGTCG GATGTCATGC AGGAAGCACT GACAGTTCAC 
TTTCAAGGTC TGTGCTTCCG TGAGCGCAAC GCCGGTAAGC GCATCGACGA GCACATGACT 
GATCGTGGAT TCAACAATCA GATAGGTGTG CATCCAGAGA CGGGCTTTGT ATTTGGCGGT 
AACGAGGCCA ATTGCGGCAC TTGGATGGAC AAGATGGGCT CCTCGGAAAA GGCAGGAAAC 
AAAGGCAAAC CTGCCACTCC CAGAGACGGA TCTGCGGTTG AAATTATTGG GCTCTGTCAC 
AGCACTCTCA ATTTTCTGGC AGAATTGTAC AAACAGAATC TATTTCCATA TGGTAGCGTG 
CAGCGGAAAA ATCGCGATGG CTCAACGATA ACTTGGAGTT ACAAACAGTG GGCGGATAAG 
ATAAAGGAGA GTTTCGATAC TTACTTCTAT GTGAACGAGA AACCTACCGA TGGTGAATTA 
CGACCGGACT TGATTCATCG ACGAGGAATT TACAAAGATA CTCACGGAGC CTCACAACCG 
TGGGCAGATT ATCAATTGCG ACCTAACTTC CCAGTGGCTA TGGTTGCTTC GCCGAACCTA 
TTCAAGCCCG AGCACGCCTG GCTGGCATTA AAGAAGGCCG AAGAAATCCT CCTTGGTCCC 
CTAGGCATGA AAACCCTAGA TCCCGCTGAC TGGGCCTACG ATGGCTTCTA CAACAATGCC 
AACGACTCGA ATGACCCGAA AGTAGCTCAC GGATGGAATT ATCACCAGGG ACCAGAGTGG 
CTATGGCCGA TCGGCTACTT TTTGCGAGCG CGCTTGCACT ACGCGCCCCT CGTTGGTGGC 
GAAGAGGAGT TAAAACGGAC TATCGCCTCG ACCGAAGCTA TCATCTCGAG ACATCTGACC 
GAAGCTTCAA ACAACCACTG GCGTGGACTT CCCGAGTTAA CCAATCGCGA GGGCGAGTAT 
TGCGCTGATA GCTGTCGCAC TCAGGCCTGG AGCGCTTCCG CAATTGTCGA GGTCCTCCAC 
GATTTGGACA AGCTGAAAAA GGATCTGAAG TTTGACGACG AGCAGGTCCT CACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008217580.1
CDS	204..4883
Translation	MENLGFSADTQNDDQVRVLTLNKGEHQDGTLYKLKKGWVVEFRLGASLLGHNLNIFTNHPLLVGNPFERRTYYQLPWNHESAFIRVSLAGSFHFYLAEASGEDAGKVIASGYLLVDPELTVGVNNQPLPLDCIQCQTVLSKLMGPFSTWEEKLRVSRKSGYNMIHFTPIQAIGDSKSAYSLSDQLKLNPSFNDAPNKETSFDDVEKLITKMREEWNTVSICDIVLNHTANESPFLINNPECSYNCNNCPHLRPSYLLDAILFELTIRVSSGEWEHKGIPPVVETEEHLNAIRHALHTYFLPLAKIHELYTVDINEIVSEFLNIARNQIPQDEPPETHNESIVIIRDPEYRRLKSTIDMQLALKIYNIYRPDCFDEDSRLKRCAEELRNKLQELNDAATNEIQNHLNAAVENSVAGIRYFRVQADGPRQKEISERNPLVPRYFTDYGSPRSFVEREDIMYSNSGCYLMAHNGWVMNSDPLKNFADADSHVYIRRELIAWGDSVKLRYGEKPEDCPALWERMTTYVQQMAKIFDGVRLDNCHSTPIPVAEHMLDAARQVRPDLYVAAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFIQARHRPLAPSIAHALFLDQTHDNPSPIEKRSVFDLLPSAALVSMACCASGSNRGYDELVPHHIHVVDEDRQYTSWTDKEGLAKENAKYVSKKSGIISAKKALNDLHFWLGQNSFSQVFVDQMDVDVVAVTRHSPTTHESVVLVAFTAFKHPDPNASDLRRHVRPLRVEGVVDEIILEASLAHGEKENGIPPFSNPKKYVKNEDYINGYSEYLVDVKEHIQICDSTKVEKVDSGDPKITQLNFINFQPGSIITIRVSLHANIKPALESINDLISTLTSPNNSELRAIVSRMNFADLNKALYRCDQEERDTTANKFGVYDIPGYGPLVYAGLQGVISLLADIRSNNDLGHPLCGNLRQGNWLIDYTWQRLKEDEGTAALGEWLEQACEPFKLIPRYLVPSYFDVIIVNVYSTLLDQCFNLMSSFVKDGTDFTRLLSLVSVQVGGIIKSAQLPDLSPNLDPPKPSIREVDGKSEQECLTMSAGLPHFTVGYMRNWGRDTFIALRGLFLLTGRHAEARFIILGYAATLRHGLIPNLLDRGVNARFNCRDAVWWWLYTIQCYVKEVPNGLKLLSDKVSRLFPTDDSDALPAGEVDETLSDVMQEALTVHFQGLCFRERNAGKRIDEHMTDRGFNNQIGVHPETGFVFGGNEANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIIGLCHSTLNFLAELYKQNLFPYGSVQRKNRDGSTITWSYKQWADKIKESFDTYFYVNEKPTDGELRPDLIHRRGIYKDTHGASQPWADYQLRPNFPVAMVASPNLFKPEHAWLALKKAEEILLGPLGMKTLDPADWAYDGFYNNANDSNDPKVAHGWNYHQGPEWLWPIGYFLRARLHYAPLVGGEEELKRTIASTEAIISRHLTEASNNHWRGLPELTNREGEYCADSCRTQAWSASAIVEVLHDLDKLKKDLKFDDEQVLTN

Target ORF information:

RefSeq Version	XM_008219358.2
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008219358.2

ORF Insert Sequence:

ATGGAAAATC TCGGCTTCTC TGCGGATACC CAGAACGACG ACCAAGTGCG AGTACTGACT 
CTGAATAAGG GAGAGCACCA GGATGGGACC CTGTACAAGC TTAAAAAAGG ATGGGTCGTG 
GAGTTCAGAT TAGGGGCATC GCTTCTGGGC CACAATCTCA ACATATTCAC TAATCATCCT 
CTACTCGTCG GCAACCCGTT CGAGAGGCGC ACGTACTATC AGCTGCCATG GAATCACGAG 
TCAGCTTTCA TACGCGTCTC GCTGGCCGGC TCTTTTCATT TTTATCTGGC AGAAGCGAGT 
GGAGAGGACG CCGGAAAAGT GATAGCTTCC GGCTACTTGT TGGTCGATCC GGAGCTAACC 
GTGGGCGTCA ACAACCAGCC GCTGCCGCTA GACTGCATAC AATGCCAGAC GGTCCTCTCA 
AAGCTCATGG GACCCTTCTC AACGTGGGAG GAGAAGCTCA GGGTCTCTCG CAAGTCAGGC 
TACAACATGA TCCACTTCAC ACCGATACAG GCGATAGGAG ACTCAAAATC AGCTTACAGC 
CTCAGCGATC AGCTGAAGCT CAACCCCTCG TTCAATGATG CACCGAACAA AGAGACCAGC 
TTCGACGACG TCGAGAAGTT GATTACAAAA ATGAGAGAAG AGTGGAACAC TGTGAGCATT 
TGCGACATAG TTTTGAATCA CACGGCGAAC GAGAGTCCAT TTTTGATAAA CAATCCCGAA 
TGTAGTTACA ACTGCAACAA CTGTCCACAT CTCCGTCCGT CTTATCTTTT GGATGCCATT 
TTATTCGAGT TGACAATACG AGTGTCGTCC GGAGAATGGG AGCACAAAGG AATTCCTCCC 
GTCGTCGAGA CTGAAGAGCA CTTGAACGCA ATCCGTCATG CTCTGCACAC TTACTTTCTA 
CCTCTGGCGA AAATTCACGA ACTGTATACT GTAGATATCA ACGAGATCGT GTCCGAGTTT 
TTGAACATTG CTCGCAACCA GATACCGCAA GACGAGCCAC CCGAAACCCA TAACGAAAGC 
ATCGTTATCA TAAGGGATCC AGAATATCGC CGCTTAAAGT CCACTATAGA TATGCAGCTT 
GCTTTGAAGA TCTACAATAT TTATAGACCC GATTGTTTTG ACGAGGACTC ACGACTGAAA 
CGCTGCGCGG AAGAACTGAG AAACAAGTTG CAGGAGTTAA ACGATGCCGC AACTAACGAA 
ATCCAAAATC ATCTCAATGC CGCGGTTGAA AACTCGGTAG CCGGCATTCG ATACTTCAGA 
GTCCAAGCAG ATGGGCCAAG ACAGAAAGAG ATAAGCGAAA GAAATCCACT TGTTCCTAGG 
TATTTCACGG ATTACGGAAG TCCTAGATCG TTTGTGGAAA GAGAAGACAT CATGTACTCG 
AATTCTGGAT GTTACTTGAT GGCTCACAAC GGCTGGGTCA TGAACAGCGA TCCCTTGAAA 
AACTTTGCCG ATGCTGACTC TCATGTTTAC ATAAGAAGGG AATTGATTGC GTGGGGCGAT 
AGTGTCAAAT TAAGATATGG CGAAAAACCG GAAGATTGCC CTGCTCTTTG GGAACGTATG 
ACGACATATG TTCAGCAAAT GGCTAAAATC TTCGACGGCG TCAGACTCGA CAATTGTCAC 
TCGACCCCTA TTCCAGTAGC AGAACACATG TTGGATGCTG CTCGTCAAGT AAGACCAGAT 
TTGTACGTTG CCGCTGAACT CTTCACCAAC TCCGATCAGA AAGATAATAT TTTCGTCAAT 
CGTCTTGGAA TCACATCGTT GATAAGAGAG GCCATGTCAG CGTGGGACAG CCATGAGGAA 
GGCCGTCTGG TATACCGCTA TGGTGGCGAG CCCGTAGGTG CGTTTATCCA GGCTCGACAT 
CGTCCTCTGG CACCGAGTAT CGCGCATGCT TTATTCCTTG ATCAGACGCA TGACAACCCG 
AGCCCCATCG AGAAGCGTAG CGTATTTGAC TTATTGCCCA GTGCCGCACT GGTCTCCATG 
GCCTGCTGTG CTAGTGGCAG TAATCGCGGT TACGACGAGC TCGTGCCTCA TCATATTCAC 
GTTGTCGATG AAGACAGACA ATATACTTCT TGGACAGATA AGGAAGGACT GGCAAAGGAA 
AATGCAAAGT ATGTTAGCAA AAAATCGGGA ATCATTTCTG CTAAAAAGGC TCTCAATGAT 
CTCCATTTTT GGCTGGGACA GAACTCCTTT TCTCAAGTTT TTGTCGATCA AATGGATGTT 
GATGTGGTAG CGGTTACAAG ACATTCACCG ACCACTCACG AGAGCGTCGT ACTCGTGGCT 
TTTACGGCAT TCAAGCACCC GGATCCCAAT GCTAGCGATT TGAGAAGACA TGTTAGACCT 
TTGAGGGTAG AAGGCGTAGT AGATGAAATT ATTCTTGAAG CATCCCTCGC GCACGGAGAA 
AAAGAAAATG GTATCCCTCC GTTCTCTAAT CCGAAGAAAT ACGTAAAGAA CGAAGACTAC 
ATAAACGGTT ACTCTGAGTA CTTGGTGGAC GTCAAGGAGC ACATTCAAAT CTGTGATTCT 
ACAAAAGTTG AAAAGGTCGA TTCCGGAGAT CCTAAAATAA CGCAATTGAA CTTTATTAAT 
TTCCAGCCCG GATCAATAAT CACTATTCGG GTGTCACTCC ACGCAAACAT AAAGCCAGCT 
CTTGAATCCA TCAATGACCT TATCTCGACC CTCACATCTC CGAACAATTC GGAATTGCGT 
GCAATTGTTT CGCGCATGAA CTTTGCGGAC CTTAACAAAG CACTTTATAG GTGTGATCAA 
GAAGAGCGAG ATACGACTGC CAATAAATTT GGAGTTTATG ATATTCCCGG TTATGGCCCA 
CTAGTTTACG CTGGTTTGCA AGGTGTAATT TCATTGCTTG CTGATATCAG ATCGAACAAC 
GATCTTGGTC ATCCACTATG TGGCAACCTT CGTCAAGGCA ATTGGTTGAT AGATTATACT 
TGGCAACGTT TGAAGGAAGA TGAAGGTACT GCAGCTCTTG GAGAGTGGCT GGAGCAGGCC 
TGCGAACCAT TCAAGCTAAT TCCTCGTTAT TTAGTGCCCA GTTACTTTGA CGTGATCATC 
GTTAACGTGT ATTCTACTCT CTTAGATCAA TGCTTTAATC TCATGTCTAG CTTTGTGAAA 
GATGGAACCG ATTTCACCAG ACTGCTGTCA CTAGTATCTG TGCAAGTTGG CGGTATAATA 
AAATCAGCGC AATTGCCAGA TTTATCACCG AACCTAGATC CACCGAAACC TAGCATACGA 
GAGGTGGATG GCAAATCAGA ACAGGAATGT TTGACCATGT CTGCTGGACT ACCACACTTT 
ACAGTAGGAT ACATGAGAAA CTGGGGACGA GATACGTTTA TCGCTTTGAG AGGTCTGTTC 
TTATTAACCG GCAGACATGC GGAGGCTCGA TTCATCATTC TGGGATACGC CGCTACGCTT 
CGTCACGGTT TGATACCAAA TCTCTTGGAC AGAGGCGTCA ACGCCAGATT CAATTGCCGA 
GATGCCGTTT GGTGGTGGTT GTACACTATT CAATGCTATG TCAAAGAAGT CCCAAATGGC 
TTAAAACTTC TGTCGGACAA AGTATCTAGA TTGTTCCCTA CTGACGATTC GGACGCTCTT 
CCAGCCGGAG AAGTGGATGA AACACTGTCG GATGTCATGC AGGAAGCACT GACAGTTCAC 
TTTCAAGGTC TGTGCTTCCG TGAGCGCAAC GCCGGTAAGC GCATCGACGA GCACATGACT 
GATCGTGGAT TCAACAATCA GATAGGTGTG CATCCAGAGA CGGGCTTTGT ATTTGGCGGT 
AACGAGGCCA ATTGCGGCAC TTGGATGGAC AAGATGGGCT CCTCGGAAAA GGCAGGAAAC 
AAAGGCAAAC CTGCCACTCC CAGAGACGGA TCTGCGGTTG AAATTATTGG GCTCTGTCAC 
AGCACTCTCA ATTTTCTGGC AGAATTGTAC AAACAGAATC TATTTCCATA TGGTAGCGTG 
CAGCGGAAAA ATCGCGATGG CTCAACGATA ACTTGGAGTT ACAAACAGTG GGCGGATAAG 
ATAAAGGAGA GTTTCGATAC TTACTTCTAT GTGAACGAGA AACCTACCGA TGGTGAATTA 
CGACCGGACT TGATTCATCG ACGAGGAATT TACAAAGATA CTCACGGAGC CTCACAACCG 
TGGGCAGATT ATCAATTGCG ACCTAACTTC CCAGTGGCTA TGGTTGCTTC GCCGAACCTA 
TTCAAGCCCG AGCACGCCTG GCTGGCATTA AAGAAGGCCG AAGAAATCCT CCTTGGTCCC 
CTAGGCATGA AAACCCTAGA TCCCGCTGAC TGGGCCTACG ATGGCTTCTA CAACAATGCC 
AACGACTCGA ATGACCCGAA AGTAGCTCAC GGATGGAATT ATCACCAGGG ACCAGAGTGG 
CTATGGCCGA TCGGCTACTT TTTGCGAGCG CGCTTGCACT ACGCGCCCCT CGTTGGTGGC 
GAAGAGGAGT TAAAACGGAC TATCGCCTCG ACCGAAGCTA TCATCTCGAG ACATCTGACC 
GAAGCTTCAA ACAACCACTG GCGTGGACTT CCCGAGTTAA CCAATCGCGA GGGCGAGTAT 
TGCGCTGATA GCTGTCGCAC TCAGGCCTGG AGCGCTTCCG CAATTGTCGA GGTCCTCCAC 
GATTTGGACA AGCTGAAAAA GGATCTGAAG TTTGACGACG AGCAGGTCCT CACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100117168 ( XM_016986671.1 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_016842160.1 Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X10, mRNA.

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CloneID	ONd11611
Clone ID Related Accession (Same CDS sequence)	XM_008219356.2 , XM_008219358.2 , XM_016986671.1 , XM_008219357.2 , XM_016986672.1
Accession Version	XM_016986671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-25
Organism	Nasonia vitripennis(jewel wasp)
Product	glycogen debranching enzyme isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001815014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATC TCGGCTTCTC TGCGGATACC CAGAACGACG ACCAAGTGCG AGTACTGACT 
CTGAATAAGG GAGAGCACCA GGATGGGACC CTGTACAAGC TTAAAAAAGG ATGGGTCGTG 
GAGTTCAGAT TAGGGGCATC GCTTCTGGGC CACAATCTCA ACATATTCAC TAATCATCCT 
CTACTCGTCG GCAACCCGTT CGAGAGGCGC ACGTACTATC AGCTGCCATG GAATCACGAG 
TCAGCTTTCA TACGCGTCTC GCTGGCCGGC TCTTTTCATT TTTATCTGGC AGAAGCGAGT 
GGAGAGGACG CCGGAAAAGT GATAGCTTCC GGCTACTTGT TGGTCGATCC GGAGCTAACC 
GTGGGCGTCA ACAACCAGCC GCTGCCGCTA GACTGCATAC AATGCCAGAC GGTCCTCTCA 
AAGCTCATGG GACCCTTCTC AACGTGGGAG GAGAAGCTCA GGGTCTCTCG CAAGTCAGGC 
TACAACATGA TCCACTTCAC ACCGATACAG GCGATAGGAG ACTCAAAATC AGCTTACAGC 
CTCAGCGATC AGCTGAAGCT CAACCCCTCG TTCAATGATG CACCGAACAA AGAGACCAGC 
TTCGACGACG TCGAGAAGTT GATTACAAAA ATGAGAGAAG AGTGGAACAC TGTGAGCATT 
TGCGACATAG TTTTGAATCA CACGGCGAAC GAGAGTCCAT TTTTGATAAA CAATCCCGAA 
TGTAGTTACA ACTGCAACAA CTGTCCACAT CTCCGTCCGT CTTATCTTTT GGATGCCATT 
TTATTCGAGT TGACAATACG AGTGTCGTCC GGAGAATGGG AGCACAAAGG AATTCCTCCC 
GTCGTCGAGA CTGAAGAGCA CTTGAACGCA ATCCGTCATG CTCTGCACAC TTACTTTCTA 
CCTCTGGCGA AAATTCACGA ACTGTATACT GTAGATATCA ACGAGATCGT GTCCGAGTTT 
TTGAACATTG CTCGCAACCA GATACCGCAA GACGAGCCAC CCGAAACCCA TAACGAAAGC 
ATCGTTATCA TAAGGGATCC AGAATATCGC CGCTTAAAGT CCACTATAGA TATGCAGCTT 
GCTTTGAAGA TCTACAATAT TTATAGACCC GATTGTTTTG ACGAGGACTC ACGACTGAAA 
CGCTGCGCGG AAGAACTGAG AAACAAGTTG CAGGAGTTAA ACGATGCCGC AACTAACGAA 
ATCCAAAATC ATCTCAATGC CGCGGTTGAA AACTCGGTAG CCGGCATTCG ATACTTCAGA 
GTCCAAGCAG ATGGGCCAAG ACAGAAAGAG ATAAGCGAAA GAAATCCACT TGTTCCTAGG 
TATTTCACGG ATTACGGAAG TCCTAGATCG TTTGTGGAAA GAGAAGACAT CATGTACTCG 
AATTCTGGAT GTTACTTGAT GGCTCACAAC GGCTGGGTCA TGAACAGCGA TCCCTTGAAA 
AACTTTGCCG ATGCTGACTC TCATGTTTAC ATAAGAAGGG AATTGATTGC GTGGGGCGAT 
AGTGTCAAAT TAAGATATGG CGAAAAACCG GAAGATTGCC CTGCTCTTTG GGAACGTATG 
ACGACATATG TTCAGCAAAT GGCTAAAATC TTCGACGGCG TCAGACTCGA CAATTGTCAC 
TCGACCCCTA TTCCAGTAGC AGAACACATG TTGGATGCTG CTCGTCAAGT AAGACCAGAT 
TTGTACGTTG CCGCTGAACT CTTCACCAAC TCCGATCAGA AAGATAATAT TTTCGTCAAT 
CGTCTTGGAA TCACATCGTT GATAAGAGAG GCCATGTCAG CGTGGGACAG CCATGAGGAA 
GGCCGTCTGG TATACCGCTA TGGTGGCGAG CCCGTAGGTG CGTTTATCCA GGCTCGACAT 
CGTCCTCTGG CACCGAGTAT CGCGCATGCT TTATTCCTTG ATCAGACGCA TGACAACCCG 
AGCCCCATCG AGAAGCGTAG CGTATTTGAC TTATTGCCCA GTGCCGCACT GGTCTCCATG 
GCCTGCTGTG CTAGTGGCAG TAATCGCGGT TACGACGAGC TCGTGCCTCA TCATATTCAC 
GTTGTCGATG AAGACAGACA ATATACTTCT TGGACAGATA AGGAAGGACT GGCAAAGGAA 
AATGCAAAGT ATGTTAGCAA AAAATCGGGA ATCATTTCTG CTAAAAAGGC TCTCAATGAT 
CTCCATTTTT GGCTGGGACA GAACTCCTTT TCTCAAGTTT TTGTCGATCA AATGGATGTT 
GATGTGGTAG CGGTTACAAG ACATTCACCG ACCACTCACG AGAGCGTCGT ACTCGTGGCT 
TTTACGGCAT TCAAGCACCC GGATCCCAAT GCTAGCGATT TGAGAAGACA TGTTAGACCT 
TTGAGGGTAG AAGGCGTAGT AGATGAAATT ATTCTTGAAG CATCCCTCGC GCACGGAGAA 
AAAGAAAATG GTATCCCTCC GTTCTCTAAT CCGAAGAAAT ACGTAAAGAA CGAAGACTAC 
ATAAACGGTT ACTCTGAGTA CTTGGTGGAC GTCAAGGAGC ACATTCAAAT CTGTGATTCT 
ACAAAAGTTG AAAAGGTCGA TTCCGGAGAT CCTAAAATAA CGCAATTGAA CTTTATTAAT 
TTCCAGCCCG GATCAATAAT CACTATTCGG GTGTCACTCC ACGCAAACAT AAAGCCAGCT 
CTTGAATCCA TCAATGACCT TATCTCGACC CTCACATCTC CGAACAATTC GGAATTGCGT 
GCAATTGTTT CGCGCATGAA CTTTGCGGAC CTTAACAAAG CACTTTATAG GTGTGATCAA 
GAAGAGCGAG ATACGACTGC CAATAAATTT GGAGTTTATG ATATTCCCGG TTATGGCCCA 
CTAGTTTACG CTGGTTTGCA AGGTGTAATT TCATTGCTTG CTGATATCAG ATCGAACAAC 
GATCTTGGTC ATCCACTATG TGGCAACCTT CGTCAAGGCA ATTGGTTGAT AGATTATACT 
TGGCAACGTT TGAAGGAAGA TGAAGGTACT GCAGCTCTTG GAGAGTGGCT GGAGCAGGCC 
TGCGAACCAT TCAAGCTAAT TCCTCGTTAT TTAGTGCCCA GTTACTTTGA CGTGATCATC 
GTTAACGTGT ATTCTACTCT CTTAGATCAA TGCTTTAATC TCATGTCTAG CTTTGTGAAA 
GATGGAACCG ATTTCACCAG ACTGCTGTCA CTAGTATCTG TGCAAGTTGG CGGTATAATA 
AAATCAGCGC AATTGCCAGA TTTATCACCG AACCTAGATC CACCGAAACC TAGCATACGA 
GAGGTGGATG GCAAATCAGA ACAGGAATGT TTGACCATGT CTGCTGGACT ACCACACTTT 
ACAGTAGGAT ACATGAGAAA CTGGGGACGA GATACGTTTA TCGCTTTGAG AGGTCTGTTC 
TTATTAACCG GCAGACATGC GGAGGCTCGA TTCATCATTC TGGGATACGC CGCTACGCTT 
CGTCACGGTT TGATACCAAA TCTCTTGGAC AGAGGCGTCA ACGCCAGATT CAATTGCCGA 
GATGCCGTTT GGTGGTGGTT GTACACTATT CAATGCTATG TCAAAGAAGT CCCAAATGGC 
TTAAAACTTC TGTCGGACAA AGTATCTAGA TTGTTCCCTA CTGACGATTC GGACGCTCTT 
CCAGCCGGAG AAGTGGATGA AACACTGTCG GATGTCATGC AGGAAGCACT GACAGTTCAC 
TTTCAAGGTC TGTGCTTCCG TGAGCGCAAC GCCGGTAAGC GCATCGACGA GCACATGACT 
GATCGTGGAT TCAACAATCA GATAGGTGTG CATCCAGAGA CGGGCTTTGT ATTTGGCGGT 
AACGAGGCCA ATTGCGGCAC TTGGATGGAC AAGATGGGCT CCTCGGAAAA GGCAGGAAAC 
AAAGGCAAAC CTGCCACTCC CAGAGACGGA TCTGCGGTTG AAATTATTGG GCTCTGTCAC 
AGCACTCTCA ATTTTCTGGC AGAATTGTAC AAACAGAATC TATTTCCATA TGGTAGCGTG 
CAGCGGAAAA ATCGCGATGG CTCAACGATA ACTTGGAGTT ACAAACAGTG GGCGGATAAG 
ATAAAGGAGA GTTTCGATAC TTACTTCTAT GTGAACGAGA AACCTACCGA TGGTGAATTA 
CGACCGGACT TGATTCATCG ACGAGGAATT TACAAAGATA CTCACGGAGC CTCACAACCG 
TGGGCAGATT ATCAATTGCG ACCTAACTTC CCAGTGGCTA TGGTTGCTTC GCCGAACCTA 
TTCAAGCCCG AGCACGCCTG GCTGGCATTA AAGAAGGCCG AAGAAATCCT CCTTGGTCCC 
CTAGGCATGA AAACCCTAGA TCCCGCTGAC TGGGCCTACG ATGGCTTCTA CAACAATGCC 
AACGACTCGA ATGACCCGAA AGTAGCTCAC GGATGGAATT ATCACCAGGG ACCAGAGTGG 
CTATGGCCGA TCGGCTACTT TTTGCGAGCG CGCTTGCACT ACGCGCCCCT CGTTGGTGGC 
GAAGAGGAGT TAAAACGGAC TATCGCCTCG ACCGAAGCTA TCATCTCGAG ACATCTGACC 
GAAGCTTCAA ACAACCACTG GCGTGGACTT CCCGAGTTAA CCAATCGCGA GGGCGAGTAT 
TGCGCTGATA GCTGTCGCAC TCAGGCCTGG AGCGCTTCCG CAATTGTCGA GGTCCTCCAC 
GATTTGGACA AGCTGAAAAA GGATCTGAAG TTTGACGACG AGCAGGTCCT CACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016842160.1
CDS	474..5153
Translation	MENLGFSADTQNDDQVRVLTLNKGEHQDGTLYKLKKGWVVEFRLGASLLGHNLNIFTNHPLLVGNPFERRTYYQLPWNHESAFIRVSLAGSFHFYLAEASGEDAGKVIASGYLLVDPELTVGVNNQPLPLDCIQCQTVLSKLMGPFSTWEEKLRVSRKSGYNMIHFTPIQAIGDSKSAYSLSDQLKLNPSFNDAPNKETSFDDVEKLITKMREEWNTVSICDIVLNHTANESPFLINNPECSYNCNNCPHLRPSYLLDAILFELTIRVSSGEWEHKGIPPVVETEEHLNAIRHALHTYFLPLAKIHELYTVDINEIVSEFLNIARNQIPQDEPPETHNESIVIIRDPEYRRLKSTIDMQLALKIYNIYRPDCFDEDSRLKRCAEELRNKLQELNDAATNEIQNHLNAAVENSVAGIRYFRVQADGPRQKEISERNPLVPRYFTDYGSPRSFVEREDIMYSNSGCYLMAHNGWVMNSDPLKNFADADSHVYIRRELIAWGDSVKLRYGEKPEDCPALWERMTTYVQQMAKIFDGVRLDNCHSTPIPVAEHMLDAARQVRPDLYVAAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFIQARHRPLAPSIAHALFLDQTHDNPSPIEKRSVFDLLPSAALVSMACCASGSNRGYDELVPHHIHVVDEDRQYTSWTDKEGLAKENAKYVSKKSGIISAKKALNDLHFWLGQNSFSQVFVDQMDVDVVAVTRHSPTTHESVVLVAFTAFKHPDPNASDLRRHVRPLRVEGVVDEIILEASLAHGEKENGIPPFSNPKKYVKNEDYINGYSEYLVDVKEHIQICDSTKVEKVDSGDPKITQLNFINFQPGSIITIRVSLHANIKPALESINDLISTLTSPNNSELRAIVSRMNFADLNKALYRCDQEERDTTANKFGVYDIPGYGPLVYAGLQGVISLLADIRSNNDLGHPLCGNLRQGNWLIDYTWQRLKEDEGTAALGEWLEQACEPFKLIPRYLVPSYFDVIIVNVYSTLLDQCFNLMSSFVKDGTDFTRLLSLVSVQVGGIIKSAQLPDLSPNLDPPKPSIREVDGKSEQECLTMSAGLPHFTVGYMRNWGRDTFIALRGLFLLTGRHAEARFIILGYAATLRHGLIPNLLDRGVNARFNCRDAVWWWLYTIQCYVKEVPNGLKLLSDKVSRLFPTDDSDALPAGEVDETLSDVMQEALTVHFQGLCFRERNAGKRIDEHMTDRGFNNQIGVHPETGFVFGGNEANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIIGLCHSTLNFLAELYKQNLFPYGSVQRKNRDGSTITWSYKQWADKIKESFDTYFYVNEKPTDGELRPDLIHRRGIYKDTHGASQPWADYQLRPNFPVAMVASPNLFKPEHAWLALKKAEEILLGPLGMKTLDPADWAYDGFYNNANDSNDPKVAHGWNYHQGPEWLWPIGYFLRARLHYAPLVGGEEELKRTIASTEAIISRHLTEASNNHWRGLPELTNREGEYCADSCRTQAWSASAIVEVLHDLDKLKKDLKFDDEQVLTN

Target ORF information:

RefSeq Version	XM_016986671.1
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016986671.1

ORF Insert Sequence:

ATGGAAAATC TCGGCTTCTC TGCGGATACC CAGAACGACG ACCAAGTGCG AGTACTGACT 
CTGAATAAGG GAGAGCACCA GGATGGGACC CTGTACAAGC TTAAAAAAGG ATGGGTCGTG 
GAGTTCAGAT TAGGGGCATC GCTTCTGGGC CACAATCTCA ACATATTCAC TAATCATCCT 
CTACTCGTCG GCAACCCGTT CGAGAGGCGC ACGTACTATC AGCTGCCATG GAATCACGAG 
TCAGCTTTCA TACGCGTCTC GCTGGCCGGC TCTTTTCATT TTTATCTGGC AGAAGCGAGT 
GGAGAGGACG CCGGAAAAGT GATAGCTTCC GGCTACTTGT TGGTCGATCC GGAGCTAACC 
GTGGGCGTCA ACAACCAGCC GCTGCCGCTA GACTGCATAC AATGCCAGAC GGTCCTCTCA 
AAGCTCATGG GACCCTTCTC AACGTGGGAG GAGAAGCTCA GGGTCTCTCG CAAGTCAGGC 
TACAACATGA TCCACTTCAC ACCGATACAG GCGATAGGAG ACTCAAAATC AGCTTACAGC 
CTCAGCGATC AGCTGAAGCT CAACCCCTCG TTCAATGATG CACCGAACAA AGAGACCAGC 
TTCGACGACG TCGAGAAGTT GATTACAAAA ATGAGAGAAG AGTGGAACAC TGTGAGCATT 
TGCGACATAG TTTTGAATCA CACGGCGAAC GAGAGTCCAT TTTTGATAAA CAATCCCGAA 
TGTAGTTACA ACTGCAACAA CTGTCCACAT CTCCGTCCGT CTTATCTTTT GGATGCCATT 
TTATTCGAGT TGACAATACG AGTGTCGTCC GGAGAATGGG AGCACAAAGG AATTCCTCCC 
GTCGTCGAGA CTGAAGAGCA CTTGAACGCA ATCCGTCATG CTCTGCACAC TTACTTTCTA 
CCTCTGGCGA AAATTCACGA ACTGTATACT GTAGATATCA ACGAGATCGT GTCCGAGTTT 
TTGAACATTG CTCGCAACCA GATACCGCAA GACGAGCCAC CCGAAACCCA TAACGAAAGC 
ATCGTTATCA TAAGGGATCC AGAATATCGC CGCTTAAAGT CCACTATAGA TATGCAGCTT 
GCTTTGAAGA TCTACAATAT TTATAGACCC GATTGTTTTG ACGAGGACTC ACGACTGAAA 
CGCTGCGCGG AAGAACTGAG AAACAAGTTG CAGGAGTTAA ACGATGCCGC AACTAACGAA 
ATCCAAAATC ATCTCAATGC CGCGGTTGAA AACTCGGTAG CCGGCATTCG ATACTTCAGA 
GTCCAAGCAG ATGGGCCAAG ACAGAAAGAG ATAAGCGAAA GAAATCCACT TGTTCCTAGG 
TATTTCACGG ATTACGGAAG TCCTAGATCG TTTGTGGAAA GAGAAGACAT CATGTACTCG 
AATTCTGGAT GTTACTTGAT GGCTCACAAC GGCTGGGTCA TGAACAGCGA TCCCTTGAAA 
AACTTTGCCG ATGCTGACTC TCATGTTTAC ATAAGAAGGG AATTGATTGC GTGGGGCGAT 
AGTGTCAAAT TAAGATATGG CGAAAAACCG GAAGATTGCC CTGCTCTTTG GGAACGTATG 
ACGACATATG TTCAGCAAAT GGCTAAAATC TTCGACGGCG TCAGACTCGA CAATTGTCAC 
TCGACCCCTA TTCCAGTAGC AGAACACATG TTGGATGCTG CTCGTCAAGT AAGACCAGAT 
TTGTACGTTG CCGCTGAACT CTTCACCAAC TCCGATCAGA AAGATAATAT TTTCGTCAAT 
CGTCTTGGAA TCACATCGTT GATAAGAGAG GCCATGTCAG CGTGGGACAG CCATGAGGAA 
GGCCGTCTGG TATACCGCTA TGGTGGCGAG CCCGTAGGTG CGTTTATCCA GGCTCGACAT 
CGTCCTCTGG CACCGAGTAT CGCGCATGCT TTATTCCTTG ATCAGACGCA TGACAACCCG 
AGCCCCATCG AGAAGCGTAG CGTATTTGAC TTATTGCCCA GTGCCGCACT GGTCTCCATG 
GCCTGCTGTG CTAGTGGCAG TAATCGCGGT TACGACGAGC TCGTGCCTCA TCATATTCAC 
GTTGTCGATG AAGACAGACA ATATACTTCT TGGACAGATA AGGAAGGACT GGCAAAGGAA 
AATGCAAAGT ATGTTAGCAA AAAATCGGGA ATCATTTCTG CTAAAAAGGC TCTCAATGAT 
CTCCATTTTT GGCTGGGACA GAACTCCTTT TCTCAAGTTT TTGTCGATCA AATGGATGTT 
GATGTGGTAG CGGTTACAAG ACATTCACCG ACCACTCACG AGAGCGTCGT ACTCGTGGCT 
TTTACGGCAT TCAAGCACCC GGATCCCAAT GCTAGCGATT TGAGAAGACA TGTTAGACCT 
TTGAGGGTAG AAGGCGTAGT AGATGAAATT ATTCTTGAAG CATCCCTCGC GCACGGAGAA 
AAAGAAAATG GTATCCCTCC GTTCTCTAAT CCGAAGAAAT ACGTAAAGAA CGAAGACTAC 
ATAAACGGTT ACTCTGAGTA CTTGGTGGAC GTCAAGGAGC ACATTCAAAT CTGTGATTCT 
ACAAAAGTTG AAAAGGTCGA TTCCGGAGAT CCTAAAATAA CGCAATTGAA CTTTATTAAT 
TTCCAGCCCG GATCAATAAT CACTATTCGG GTGTCACTCC ACGCAAACAT AAAGCCAGCT 
CTTGAATCCA TCAATGACCT TATCTCGACC CTCACATCTC CGAACAATTC GGAATTGCGT 
GCAATTGTTT CGCGCATGAA CTTTGCGGAC CTTAACAAAG CACTTTATAG GTGTGATCAA 
GAAGAGCGAG ATACGACTGC CAATAAATTT GGAGTTTATG ATATTCCCGG TTATGGCCCA 
CTAGTTTACG CTGGTTTGCA AGGTGTAATT TCATTGCTTG CTGATATCAG ATCGAACAAC 
GATCTTGGTC ATCCACTATG TGGCAACCTT CGTCAAGGCA ATTGGTTGAT AGATTATACT 
TGGCAACGTT TGAAGGAAGA TGAAGGTACT GCAGCTCTTG GAGAGTGGCT GGAGCAGGCC 
TGCGAACCAT TCAAGCTAAT TCCTCGTTAT TTAGTGCCCA GTTACTTTGA CGTGATCATC 
GTTAACGTGT ATTCTACTCT CTTAGATCAA TGCTTTAATC TCATGTCTAG CTTTGTGAAA 
GATGGAACCG ATTTCACCAG ACTGCTGTCA CTAGTATCTG TGCAAGTTGG CGGTATAATA 
AAATCAGCGC AATTGCCAGA TTTATCACCG AACCTAGATC CACCGAAACC TAGCATACGA 
GAGGTGGATG GCAAATCAGA ACAGGAATGT TTGACCATGT CTGCTGGACT ACCACACTTT 
ACAGTAGGAT ACATGAGAAA CTGGGGACGA GATACGTTTA TCGCTTTGAG AGGTCTGTTC 
TTATTAACCG GCAGACATGC GGAGGCTCGA TTCATCATTC TGGGATACGC CGCTACGCTT 
CGTCACGGTT TGATACCAAA TCTCTTGGAC AGAGGCGTCA ACGCCAGATT CAATTGCCGA 
GATGCCGTTT GGTGGTGGTT GTACACTATT CAATGCTATG TCAAAGAAGT CCCAAATGGC 
TTAAAACTTC TGTCGGACAA AGTATCTAGA TTGTTCCCTA CTGACGATTC GGACGCTCTT 
CCAGCCGGAG AAGTGGATGA AACACTGTCG GATGTCATGC AGGAAGCACT GACAGTTCAC 
TTTCAAGGTC TGTGCTTCCG TGAGCGCAAC GCCGGTAAGC GCATCGACGA GCACATGACT 
GATCGTGGAT TCAACAATCA GATAGGTGTG CATCCAGAGA CGGGCTTTGT ATTTGGCGGT 
AACGAGGCCA ATTGCGGCAC TTGGATGGAC AAGATGGGCT CCTCGGAAAA GGCAGGAAAC 
AAAGGCAAAC CTGCCACTCC CAGAGACGGA TCTGCGGTTG AAATTATTGG GCTCTGTCAC 
AGCACTCTCA ATTTTCTGGC AGAATTGTAC AAACAGAATC TATTTCCATA TGGTAGCGTG 
CAGCGGAAAA ATCGCGATGG CTCAACGATA ACTTGGAGTT ACAAACAGTG GGCGGATAAG 
ATAAAGGAGA GTTTCGATAC TTACTTCTAT GTGAACGAGA AACCTACCGA TGGTGAATTA 
CGACCGGACT TGATTCATCG ACGAGGAATT TACAAAGATA CTCACGGAGC CTCACAACCG 
TGGGCAGATT ATCAATTGCG ACCTAACTTC CCAGTGGCTA TGGTTGCTTC GCCGAACCTA 
TTCAAGCCCG AGCACGCCTG GCTGGCATTA AAGAAGGCCG AAGAAATCCT CCTTGGTCCC 
CTAGGCATGA AAACCCTAGA TCCCGCTGAC TGGGCCTACG ATGGCTTCTA CAACAATGCC 
AACGACTCGA ATGACCCGAA AGTAGCTCAC GGATGGAATT ATCACCAGGG ACCAGAGTGG 
CTATGGCCGA TCGGCTACTT TTTGCGAGCG CGCTTGCACT ACGCGCCCCT CGTTGGTGGC 
GAAGAGGAGT TAAAACGGAC TATCGCCTCG ACCGAAGCTA TCATCTCGAG ACATCTGACC 
GAAGCTTCAA ACAACCACTG GCGTGGACTT CCCGAGTTAA CCAATCGCGA GGGCGAGTAT 
TGCGCTGATA GCTGTCGCAC TCAGGCCTGG AGCGCTTCCG CAATTGTCGA GGTCCTCCAC 
GATTTGGACA AGCTGAAAAA GGATCTGAAG TTTGACGACG AGCAGGTCCT CACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100117168 ( XM_008219357.2 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_008217579.1 Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X8, mRNA.

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CloneID	ONd11611
Clone ID Related Accession (Same CDS sequence)	XM_008219356.2 , XM_008219358.2 , XM_016986671.1 , XM_008219357.2 , XM_016986672.1
Accession Version	XM_008219357.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-25
Organism	Nasonia vitripennis(jewel wasp)
Product	glycogen debranching enzyme isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001815014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2016 this sequence version replaced XM_008219357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATC TCGGCTTCTC TGCGGATACC CAGAACGACG ACCAAGTGCG AGTACTGACT 
CTGAATAAGG GAGAGCACCA GGATGGGACC CTGTACAAGC TTAAAAAAGG ATGGGTCGTG 
GAGTTCAGAT TAGGGGCATC GCTTCTGGGC CACAATCTCA ACATATTCAC TAATCATCCT 
CTACTCGTCG GCAACCCGTT CGAGAGGCGC ACGTACTATC AGCTGCCATG GAATCACGAG 
TCAGCTTTCA TACGCGTCTC GCTGGCCGGC TCTTTTCATT TTTATCTGGC AGAAGCGAGT 
GGAGAGGACG CCGGAAAAGT GATAGCTTCC GGCTACTTGT TGGTCGATCC GGAGCTAACC 
GTGGGCGTCA ACAACCAGCC GCTGCCGCTA GACTGCATAC AATGCCAGAC GGTCCTCTCA 
AAGCTCATGG GACCCTTCTC AACGTGGGAG GAGAAGCTCA GGGTCTCTCG CAAGTCAGGC 
TACAACATGA TCCACTTCAC ACCGATACAG GCGATAGGAG ACTCAAAATC AGCTTACAGC 
CTCAGCGATC AGCTGAAGCT CAACCCCTCG TTCAATGATG CACCGAACAA AGAGACCAGC 
TTCGACGACG TCGAGAAGTT GATTACAAAA ATGAGAGAAG AGTGGAACAC TGTGAGCATT 
TGCGACATAG TTTTGAATCA CACGGCGAAC GAGAGTCCAT TTTTGATAAA CAATCCCGAA 
TGTAGTTACA ACTGCAACAA CTGTCCACAT CTCCGTCCGT CTTATCTTTT GGATGCCATT 
TTATTCGAGT TGACAATACG AGTGTCGTCC GGAGAATGGG AGCACAAAGG AATTCCTCCC 
GTCGTCGAGA CTGAAGAGCA CTTGAACGCA ATCCGTCATG CTCTGCACAC TTACTTTCTA 
CCTCTGGCGA AAATTCACGA ACTGTATACT GTAGATATCA ACGAGATCGT GTCCGAGTTT 
TTGAACATTG CTCGCAACCA GATACCGCAA GACGAGCCAC CCGAAACCCA TAACGAAAGC 
ATCGTTATCA TAAGGGATCC AGAATATCGC CGCTTAAAGT CCACTATAGA TATGCAGCTT 
GCTTTGAAGA TCTACAATAT TTATAGACCC GATTGTTTTG ACGAGGACTC ACGACTGAAA 
CGCTGCGCGG AAGAACTGAG AAACAAGTTG CAGGAGTTAA ACGATGCCGC AACTAACGAA 
ATCCAAAATC ATCTCAATGC CGCGGTTGAA AACTCGGTAG CCGGCATTCG ATACTTCAGA 
GTCCAAGCAG ATGGGCCAAG ACAGAAAGAG ATAAGCGAAA GAAATCCACT TGTTCCTAGG 
TATTTCACGG ATTACGGAAG TCCTAGATCG TTTGTGGAAA GAGAAGACAT CATGTACTCG 
AATTCTGGAT GTTACTTGAT GGCTCACAAC GGCTGGGTCA TGAACAGCGA TCCCTTGAAA 
AACTTTGCCG ATGCTGACTC TCATGTTTAC ATAAGAAGGG AATTGATTGC GTGGGGCGAT 
AGTGTCAAAT TAAGATATGG CGAAAAACCG GAAGATTGCC CTGCTCTTTG GGAACGTATG 
ACGACATATG TTCAGCAAAT GGCTAAAATC TTCGACGGCG TCAGACTCGA CAATTGTCAC 
TCGACCCCTA TTCCAGTAGC AGAACACATG TTGGATGCTG CTCGTCAAGT AAGACCAGAT 
TTGTACGTTG CCGCTGAACT CTTCACCAAC TCCGATCAGA AAGATAATAT TTTCGTCAAT 
CGTCTTGGAA TCACATCGTT GATAAGAGAG GCCATGTCAG CGTGGGACAG CCATGAGGAA 
GGCCGTCTGG TATACCGCTA TGGTGGCGAG CCCGTAGGTG CGTTTATCCA GGCTCGACAT 
CGTCCTCTGG CACCGAGTAT CGCGCATGCT TTATTCCTTG ATCAGACGCA TGACAACCCG 
AGCCCCATCG AGAAGCGTAG CGTATTTGAC TTATTGCCCA GTGCCGCACT GGTCTCCATG 
GCCTGCTGTG CTAGTGGCAG TAATCGCGGT TACGACGAGC TCGTGCCTCA TCATATTCAC 
GTTGTCGATG AAGACAGACA ATATACTTCT TGGACAGATA AGGAAGGACT GGCAAAGGAA 
AATGCAAAGT ATGTTAGCAA AAAATCGGGA ATCATTTCTG CTAAAAAGGC TCTCAATGAT 
CTCCATTTTT GGCTGGGACA GAACTCCTTT TCTCAAGTTT TTGTCGATCA AATGGATGTT 
GATGTGGTAG CGGTTACAAG ACATTCACCG ACCACTCACG AGAGCGTCGT ACTCGTGGCT 
TTTACGGCAT TCAAGCACCC GGATCCCAAT GCTAGCGATT TGAGAAGACA TGTTAGACCT 
TTGAGGGTAG AAGGCGTAGT AGATGAAATT ATTCTTGAAG CATCCCTCGC GCACGGAGAA 
AAAGAAAATG GTATCCCTCC GTTCTCTAAT CCGAAGAAAT ACGTAAAGAA CGAAGACTAC 
ATAAACGGTT ACTCTGAGTA CTTGGTGGAC GTCAAGGAGC ACATTCAAAT CTGTGATTCT 
ACAAAAGTTG AAAAGGTCGA TTCCGGAGAT CCTAAAATAA CGCAATTGAA CTTTATTAAT 
TTCCAGCCCG GATCAATAAT CACTATTCGG GTGTCACTCC ACGCAAACAT AAAGCCAGCT 
CTTGAATCCA TCAATGACCT TATCTCGACC CTCACATCTC CGAACAATTC GGAATTGCGT 
GCAATTGTTT CGCGCATGAA CTTTGCGGAC CTTAACAAAG CACTTTATAG GTGTGATCAA 
GAAGAGCGAG ATACGACTGC CAATAAATTT GGAGTTTATG ATATTCCCGG TTATGGCCCA 
CTAGTTTACG CTGGTTTGCA AGGTGTAATT TCATTGCTTG CTGATATCAG ATCGAACAAC 
GATCTTGGTC ATCCACTATG TGGCAACCTT CGTCAAGGCA ATTGGTTGAT AGATTATACT 
TGGCAACGTT TGAAGGAAGA TGAAGGTACT GCAGCTCTTG GAGAGTGGCT GGAGCAGGCC 
TGCGAACCAT TCAAGCTAAT TCCTCGTTAT TTAGTGCCCA GTTACTTTGA CGTGATCATC 
GTTAACGTGT ATTCTACTCT CTTAGATCAA TGCTTTAATC TCATGTCTAG CTTTGTGAAA 
GATGGAACCG ATTTCACCAG ACTGCTGTCA CTAGTATCTG TGCAAGTTGG CGGTATAATA 
AAATCAGCGC AATTGCCAGA TTTATCACCG AACCTAGATC CACCGAAACC TAGCATACGA 
GAGGTGGATG GCAAATCAGA ACAGGAATGT TTGACCATGT CTGCTGGACT ACCACACTTT 
ACAGTAGGAT ACATGAGAAA CTGGGGACGA GATACGTTTA TCGCTTTGAG AGGTCTGTTC 
TTATTAACCG GCAGACATGC GGAGGCTCGA TTCATCATTC TGGGATACGC CGCTACGCTT 
CGTCACGGTT TGATACCAAA TCTCTTGGAC AGAGGCGTCA ACGCCAGATT CAATTGCCGA 
GATGCCGTTT GGTGGTGGTT GTACACTATT CAATGCTATG TCAAAGAAGT CCCAAATGGC 
TTAAAACTTC TGTCGGACAA AGTATCTAGA TTGTTCCCTA CTGACGATTC GGACGCTCTT 
CCAGCCGGAG AAGTGGATGA AACACTGTCG GATGTCATGC AGGAAGCACT GACAGTTCAC 
TTTCAAGGTC TGTGCTTCCG TGAGCGCAAC GCCGGTAAGC GCATCGACGA GCACATGACT 
GATCGTGGAT TCAACAATCA GATAGGTGTG CATCCAGAGA CGGGCTTTGT ATTTGGCGGT 
AACGAGGCCA ATTGCGGCAC TTGGATGGAC AAGATGGGCT CCTCGGAAAA GGCAGGAAAC 
AAAGGCAAAC CTGCCACTCC CAGAGACGGA TCTGCGGTTG AAATTATTGG GCTCTGTCAC 
AGCACTCTCA ATTTTCTGGC AGAATTGTAC AAACAGAATC TATTTCCATA TGGTAGCGTG 
CAGCGGAAAA ATCGCGATGG CTCAACGATA ACTTGGAGTT ACAAACAGTG GGCGGATAAG 
ATAAAGGAGA GTTTCGATAC TTACTTCTAT GTGAACGAGA AACCTACCGA TGGTGAATTA 
CGACCGGACT TGATTCATCG ACGAGGAATT TACAAAGATA CTCACGGAGC CTCACAACCG 
TGGGCAGATT ATCAATTGCG ACCTAACTTC CCAGTGGCTA TGGTTGCTTC GCCGAACCTA 
TTCAAGCCCG AGCACGCCTG GCTGGCATTA AAGAAGGCCG AAGAAATCCT CCTTGGTCCC 
CTAGGCATGA AAACCCTAGA TCCCGCTGAC TGGGCCTACG ATGGCTTCTA CAACAATGCC 
AACGACTCGA ATGACCCGAA AGTAGCTCAC GGATGGAATT ATCACCAGGG ACCAGAGTGG 
CTATGGCCGA TCGGCTACTT TTTGCGAGCG CGCTTGCACT ACGCGCCCCT CGTTGGTGGC 
GAAGAGGAGT TAAAACGGAC TATCGCCTCG ACCGAAGCTA TCATCTCGAG ACATCTGACC 
GAAGCTTCAA ACAACCACTG GCGTGGACTT CCCGAGTTAA CCAATCGCGA GGGCGAGTAT 
TGCGCTGATA GCTGTCGCAC TCAGGCCTGG AGCGCTTCCG CAATTGTCGA GGTCCTCCAC 
GATTTGGACA AGCTGAAAAA GGATCTGAAG TTTGACGACG AGCAGGTCCT CACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008217579.1
CDS	213..4892
Translation	MENLGFSADTQNDDQVRVLTLNKGEHQDGTLYKLKKGWVVEFRLGASLLGHNLNIFTNHPLLVGNPFERRTYYQLPWNHESAFIRVSLAGSFHFYLAEASGEDAGKVIASGYLLVDPELTVGVNNQPLPLDCIQCQTVLSKLMGPFSTWEEKLRVSRKSGYNMIHFTPIQAIGDSKSAYSLSDQLKLNPSFNDAPNKETSFDDVEKLITKMREEWNTVSICDIVLNHTANESPFLINNPECSYNCNNCPHLRPSYLLDAILFELTIRVSSGEWEHKGIPPVVETEEHLNAIRHALHTYFLPLAKIHELYTVDINEIVSEFLNIARNQIPQDEPPETHNESIVIIRDPEYRRLKSTIDMQLALKIYNIYRPDCFDEDSRLKRCAEELRNKLQELNDAATNEIQNHLNAAVENSVAGIRYFRVQADGPRQKEISERNPLVPRYFTDYGSPRSFVEREDIMYSNSGCYLMAHNGWVMNSDPLKNFADADSHVYIRRELIAWGDSVKLRYGEKPEDCPALWERMTTYVQQMAKIFDGVRLDNCHSTPIPVAEHMLDAARQVRPDLYVAAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFIQARHRPLAPSIAHALFLDQTHDNPSPIEKRSVFDLLPSAALVSMACCASGSNRGYDELVPHHIHVVDEDRQYTSWTDKEGLAKENAKYVSKKSGIISAKKALNDLHFWLGQNSFSQVFVDQMDVDVVAVTRHSPTTHESVVLVAFTAFKHPDPNASDLRRHVRPLRVEGVVDEIILEASLAHGEKENGIPPFSNPKKYVKNEDYINGYSEYLVDVKEHIQICDSTKVEKVDSGDPKITQLNFINFQPGSIITIRVSLHANIKPALESINDLISTLTSPNNSELRAIVSRMNFADLNKALYRCDQEERDTTANKFGVYDIPGYGPLVYAGLQGVISLLADIRSNNDLGHPLCGNLRQGNWLIDYTWQRLKEDEGTAALGEWLEQACEPFKLIPRYLVPSYFDVIIVNVYSTLLDQCFNLMSSFVKDGTDFTRLLSLVSVQVGGIIKSAQLPDLSPNLDPPKPSIREVDGKSEQECLTMSAGLPHFTVGYMRNWGRDTFIALRGLFLLTGRHAEARFIILGYAATLRHGLIPNLLDRGVNARFNCRDAVWWWLYTIQCYVKEVPNGLKLLSDKVSRLFPTDDSDALPAGEVDETLSDVMQEALTVHFQGLCFRERNAGKRIDEHMTDRGFNNQIGVHPETGFVFGGNEANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIIGLCHSTLNFLAELYKQNLFPYGSVQRKNRDGSTITWSYKQWADKIKESFDTYFYVNEKPTDGELRPDLIHRRGIYKDTHGASQPWADYQLRPNFPVAMVASPNLFKPEHAWLALKKAEEILLGPLGMKTLDPADWAYDGFYNNANDSNDPKVAHGWNYHQGPEWLWPIGYFLRARLHYAPLVGGEEELKRTIASTEAIISRHLTEASNNHWRGLPELTNREGEYCADSCRTQAWSASAIVEVLHDLDKLKKDLKFDDEQVLTN

Target ORF information:

RefSeq Version	XM_008219357.2
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008219357.2

ORF Insert Sequence:

ATGGAAAATC TCGGCTTCTC TGCGGATACC CAGAACGACG ACCAAGTGCG AGTACTGACT 
CTGAATAAGG GAGAGCACCA GGATGGGACC CTGTACAAGC TTAAAAAAGG ATGGGTCGTG 
GAGTTCAGAT TAGGGGCATC GCTTCTGGGC CACAATCTCA ACATATTCAC TAATCATCCT 
CTACTCGTCG GCAACCCGTT CGAGAGGCGC ACGTACTATC AGCTGCCATG GAATCACGAG 
TCAGCTTTCA TACGCGTCTC GCTGGCCGGC TCTTTTCATT TTTATCTGGC AGAAGCGAGT 
GGAGAGGACG CCGGAAAAGT GATAGCTTCC GGCTACTTGT TGGTCGATCC GGAGCTAACC 
GTGGGCGTCA ACAACCAGCC GCTGCCGCTA GACTGCATAC AATGCCAGAC GGTCCTCTCA 
AAGCTCATGG GACCCTTCTC AACGTGGGAG GAGAAGCTCA GGGTCTCTCG CAAGTCAGGC 
TACAACATGA TCCACTTCAC ACCGATACAG GCGATAGGAG ACTCAAAATC AGCTTACAGC 
CTCAGCGATC AGCTGAAGCT CAACCCCTCG TTCAATGATG CACCGAACAA AGAGACCAGC 
TTCGACGACG TCGAGAAGTT GATTACAAAA ATGAGAGAAG AGTGGAACAC TGTGAGCATT 
TGCGACATAG TTTTGAATCA CACGGCGAAC GAGAGTCCAT TTTTGATAAA CAATCCCGAA 
TGTAGTTACA ACTGCAACAA CTGTCCACAT CTCCGTCCGT CTTATCTTTT GGATGCCATT 
TTATTCGAGT TGACAATACG AGTGTCGTCC GGAGAATGGG AGCACAAAGG AATTCCTCCC 
GTCGTCGAGA CTGAAGAGCA CTTGAACGCA ATCCGTCATG CTCTGCACAC TTACTTTCTA 
CCTCTGGCGA AAATTCACGA ACTGTATACT GTAGATATCA ACGAGATCGT GTCCGAGTTT 
TTGAACATTG CTCGCAACCA GATACCGCAA GACGAGCCAC CCGAAACCCA TAACGAAAGC 
ATCGTTATCA TAAGGGATCC AGAATATCGC CGCTTAAAGT CCACTATAGA TATGCAGCTT 
GCTTTGAAGA TCTACAATAT TTATAGACCC GATTGTTTTG ACGAGGACTC ACGACTGAAA 
CGCTGCGCGG AAGAACTGAG AAACAAGTTG CAGGAGTTAA ACGATGCCGC AACTAACGAA 
ATCCAAAATC ATCTCAATGC CGCGGTTGAA AACTCGGTAG CCGGCATTCG ATACTTCAGA 
GTCCAAGCAG ATGGGCCAAG ACAGAAAGAG ATAAGCGAAA GAAATCCACT TGTTCCTAGG 
TATTTCACGG ATTACGGAAG TCCTAGATCG TTTGTGGAAA GAGAAGACAT CATGTACTCG 
AATTCTGGAT GTTACTTGAT GGCTCACAAC GGCTGGGTCA TGAACAGCGA TCCCTTGAAA 
AACTTTGCCG ATGCTGACTC TCATGTTTAC ATAAGAAGGG AATTGATTGC GTGGGGCGAT 
AGTGTCAAAT TAAGATATGG CGAAAAACCG GAAGATTGCC CTGCTCTTTG GGAACGTATG 
ACGACATATG TTCAGCAAAT GGCTAAAATC TTCGACGGCG TCAGACTCGA CAATTGTCAC 
TCGACCCCTA TTCCAGTAGC AGAACACATG TTGGATGCTG CTCGTCAAGT AAGACCAGAT 
TTGTACGTTG CCGCTGAACT CTTCACCAAC TCCGATCAGA AAGATAATAT TTTCGTCAAT 
CGTCTTGGAA TCACATCGTT GATAAGAGAG GCCATGTCAG CGTGGGACAG CCATGAGGAA 
GGCCGTCTGG TATACCGCTA TGGTGGCGAG CCCGTAGGTG CGTTTATCCA GGCTCGACAT 
CGTCCTCTGG CACCGAGTAT CGCGCATGCT TTATTCCTTG ATCAGACGCA TGACAACCCG 
AGCCCCATCG AGAAGCGTAG CGTATTTGAC TTATTGCCCA GTGCCGCACT GGTCTCCATG 
GCCTGCTGTG CTAGTGGCAG TAATCGCGGT TACGACGAGC TCGTGCCTCA TCATATTCAC 
GTTGTCGATG AAGACAGACA ATATACTTCT TGGACAGATA AGGAAGGACT GGCAAAGGAA 
AATGCAAAGT ATGTTAGCAA AAAATCGGGA ATCATTTCTG CTAAAAAGGC TCTCAATGAT 
CTCCATTTTT GGCTGGGACA GAACTCCTTT TCTCAAGTTT TTGTCGATCA AATGGATGTT 
GATGTGGTAG CGGTTACAAG ACATTCACCG ACCACTCACG AGAGCGTCGT ACTCGTGGCT 
TTTACGGCAT TCAAGCACCC GGATCCCAAT GCTAGCGATT TGAGAAGACA TGTTAGACCT 
TTGAGGGTAG AAGGCGTAGT AGATGAAATT ATTCTTGAAG CATCCCTCGC GCACGGAGAA 
AAAGAAAATG GTATCCCTCC GTTCTCTAAT CCGAAGAAAT ACGTAAAGAA CGAAGACTAC 
ATAAACGGTT ACTCTGAGTA CTTGGTGGAC GTCAAGGAGC ACATTCAAAT CTGTGATTCT 
ACAAAAGTTG AAAAGGTCGA TTCCGGAGAT CCTAAAATAA CGCAATTGAA CTTTATTAAT 
TTCCAGCCCG GATCAATAAT CACTATTCGG GTGTCACTCC ACGCAAACAT AAAGCCAGCT 
CTTGAATCCA TCAATGACCT TATCTCGACC CTCACATCTC CGAACAATTC GGAATTGCGT 
GCAATTGTTT CGCGCATGAA CTTTGCGGAC CTTAACAAAG CACTTTATAG GTGTGATCAA 
GAAGAGCGAG ATACGACTGC CAATAAATTT GGAGTTTATG ATATTCCCGG TTATGGCCCA 
CTAGTTTACG CTGGTTTGCA AGGTGTAATT TCATTGCTTG CTGATATCAG ATCGAACAAC 
GATCTTGGTC ATCCACTATG TGGCAACCTT CGTCAAGGCA ATTGGTTGAT AGATTATACT 
TGGCAACGTT TGAAGGAAGA TGAAGGTACT GCAGCTCTTG GAGAGTGGCT GGAGCAGGCC 
TGCGAACCAT TCAAGCTAAT TCCTCGTTAT TTAGTGCCCA GTTACTTTGA CGTGATCATC 
GTTAACGTGT ATTCTACTCT CTTAGATCAA TGCTTTAATC TCATGTCTAG CTTTGTGAAA 
GATGGAACCG ATTTCACCAG ACTGCTGTCA CTAGTATCTG TGCAAGTTGG CGGTATAATA 
AAATCAGCGC AATTGCCAGA TTTATCACCG AACCTAGATC CACCGAAACC TAGCATACGA 
GAGGTGGATG GCAAATCAGA ACAGGAATGT TTGACCATGT CTGCTGGACT ACCACACTTT 
ACAGTAGGAT ACATGAGAAA CTGGGGACGA GATACGTTTA TCGCTTTGAG AGGTCTGTTC 
TTATTAACCG GCAGACATGC GGAGGCTCGA TTCATCATTC TGGGATACGC CGCTACGCTT 
CGTCACGGTT TGATACCAAA TCTCTTGGAC AGAGGCGTCA ACGCCAGATT CAATTGCCGA 
GATGCCGTTT GGTGGTGGTT GTACACTATT CAATGCTATG TCAAAGAAGT CCCAAATGGC 
TTAAAACTTC TGTCGGACAA AGTATCTAGA TTGTTCCCTA CTGACGATTC GGACGCTCTT 
CCAGCCGGAG AAGTGGATGA AACACTGTCG GATGTCATGC AGGAAGCACT GACAGTTCAC 
TTTCAAGGTC TGTGCTTCCG TGAGCGCAAC GCCGGTAAGC GCATCGACGA GCACATGACT 
GATCGTGGAT TCAACAATCA GATAGGTGTG CATCCAGAGA CGGGCTTTGT ATTTGGCGGT 
AACGAGGCCA ATTGCGGCAC TTGGATGGAC AAGATGGGCT CCTCGGAAAA GGCAGGAAAC 
AAAGGCAAAC CTGCCACTCC CAGAGACGGA TCTGCGGTTG AAATTATTGG GCTCTGTCAC 
AGCACTCTCA ATTTTCTGGC AGAATTGTAC AAACAGAATC TATTTCCATA TGGTAGCGTG 
CAGCGGAAAA ATCGCGATGG CTCAACGATA ACTTGGAGTT ACAAACAGTG GGCGGATAAG 
ATAAAGGAGA GTTTCGATAC TTACTTCTAT GTGAACGAGA AACCTACCGA TGGTGAATTA 
CGACCGGACT TGATTCATCG ACGAGGAATT TACAAAGATA CTCACGGAGC CTCACAACCG 
TGGGCAGATT ATCAATTGCG ACCTAACTTC CCAGTGGCTA TGGTTGCTTC GCCGAACCTA 
TTCAAGCCCG AGCACGCCTG GCTGGCATTA AAGAAGGCCG AAGAAATCCT CCTTGGTCCC 
CTAGGCATGA AAACCCTAGA TCCCGCTGAC TGGGCCTACG ATGGCTTCTA CAACAATGCC 
AACGACTCGA ATGACCCGAA AGTAGCTCAC GGATGGAATT ATCACCAGGG ACCAGAGTGG 
CTATGGCCGA TCGGCTACTT TTTGCGAGCG CGCTTGCACT ACGCGCCCCT CGTTGGTGGC 
GAAGAGGAGT TAAAACGGAC TATCGCCTCG ACCGAAGCTA TCATCTCGAG ACATCTGACC 
GAAGCTTCAA ACAACCACTG GCGTGGACTT CCCGAGTTAA CCAATCGCGA GGGCGAGTAT 
TGCGCTGATA GCTGTCGCAC TCAGGCCTGG AGCGCTTCCG CAATTGTCGA GGTCCTCCAC 
GATTTGGACA AGCTGAAAAA GGATCTGAAG TTTGACGACG AGCAGGTCCT CACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100117168 ( XM_016986672.1 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_016842161.1 Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X11, mRNA.

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CloneID	ONd11611
Clone ID Related Accession (Same CDS sequence)	XM_008219356.2 , XM_008219358.2 , XM_016986671.1 , XM_008219357.2 , XM_016986672.1
Accession Version	XM_016986672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-25
Organism	Nasonia vitripennis(jewel wasp)
Product	glycogen debranching enzyme isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001815014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATC TCGGCTTCTC TGCGGATACC CAGAACGACG ACCAAGTGCG AGTACTGACT 
CTGAATAAGG GAGAGCACCA GGATGGGACC CTGTACAAGC TTAAAAAAGG ATGGGTCGTG 
GAGTTCAGAT TAGGGGCATC GCTTCTGGGC CACAATCTCA ACATATTCAC TAATCATCCT 
CTACTCGTCG GCAACCCGTT CGAGAGGCGC ACGTACTATC AGCTGCCATG GAATCACGAG 
TCAGCTTTCA TACGCGTCTC GCTGGCCGGC TCTTTTCATT TTTATCTGGC AGAAGCGAGT 
GGAGAGGACG CCGGAAAAGT GATAGCTTCC GGCTACTTGT TGGTCGATCC GGAGCTAACC 
GTGGGCGTCA ACAACCAGCC GCTGCCGCTA GACTGCATAC AATGCCAGAC GGTCCTCTCA 
AAGCTCATGG GACCCTTCTC AACGTGGGAG GAGAAGCTCA GGGTCTCTCG CAAGTCAGGC 
TACAACATGA TCCACTTCAC ACCGATACAG GCGATAGGAG ACTCAAAATC AGCTTACAGC 
CTCAGCGATC AGCTGAAGCT CAACCCCTCG TTCAATGATG CACCGAACAA AGAGACCAGC 
TTCGACGACG TCGAGAAGTT GATTACAAAA ATGAGAGAAG AGTGGAACAC TGTGAGCATT 
TGCGACATAG TTTTGAATCA CACGGCGAAC GAGAGTCCAT TTTTGATAAA CAATCCCGAA 
TGTAGTTACA ACTGCAACAA CTGTCCACAT CTCCGTCCGT CTTATCTTTT GGATGCCATT 
TTATTCGAGT TGACAATACG AGTGTCGTCC GGAGAATGGG AGCACAAAGG AATTCCTCCC 
GTCGTCGAGA CTGAAGAGCA CTTGAACGCA ATCCGTCATG CTCTGCACAC TTACTTTCTA 
CCTCTGGCGA AAATTCACGA ACTGTATACT GTAGATATCA ACGAGATCGT GTCCGAGTTT 
TTGAACATTG CTCGCAACCA GATACCGCAA GACGAGCCAC CCGAAACCCA TAACGAAAGC 
ATCGTTATCA TAAGGGATCC AGAATATCGC CGCTTAAAGT CCACTATAGA TATGCAGCTT 
GCTTTGAAGA TCTACAATAT TTATAGACCC GATTGTTTTG ACGAGGACTC ACGACTGAAA 
CGCTGCGCGG AAGAACTGAG AAACAAGTTG CAGGAGTTAA ACGATGCCGC AACTAACGAA 
ATCCAAAATC ATCTCAATGC CGCGGTTGAA AACTCGGTAG CCGGCATTCG ATACTTCAGA 
GTCCAAGCAG ATGGGCCAAG ACAGAAAGAG ATAAGCGAAA GAAATCCACT TGTTCCTAGG 
TATTTCACGG ATTACGGAAG TCCTAGATCG TTTGTGGAAA GAGAAGACAT CATGTACTCG 
AATTCTGGAT GTTACTTGAT GGCTCACAAC GGCTGGGTCA TGAACAGCGA TCCCTTGAAA 
AACTTTGCCG ATGCTGACTC TCATGTTTAC ATAAGAAGGG AATTGATTGC GTGGGGCGAT 
AGTGTCAAAT TAAGATATGG CGAAAAACCG GAAGATTGCC CTGCTCTTTG GGAACGTATG 
ACGACATATG TTCAGCAAAT GGCTAAAATC TTCGACGGCG TCAGACTCGA CAATTGTCAC 
TCGACCCCTA TTCCAGTAGC AGAACACATG TTGGATGCTG CTCGTCAAGT AAGACCAGAT 
TTGTACGTTG CCGCTGAACT CTTCACCAAC TCCGATCAGA AAGATAATAT TTTCGTCAAT 
CGTCTTGGAA TCACATCGTT GATAAGAGAG GCCATGTCAG CGTGGGACAG CCATGAGGAA 
GGCCGTCTGG TATACCGCTA TGGTGGCGAG CCCGTAGGTG CGTTTATCCA GGCTCGACAT 
CGTCCTCTGG CACCGAGTAT CGCGCATGCT TTATTCCTTG ATCAGACGCA TGACAACCCG 
AGCCCCATCG AGAAGCGTAG CGTATTTGAC TTATTGCCCA GTGCCGCACT GGTCTCCATG 
GCCTGCTGTG CTAGTGGCAG TAATCGCGGT TACGACGAGC TCGTGCCTCA TCATATTCAC 
GTTGTCGATG AAGACAGACA ATATACTTCT TGGACAGATA AGGAAGGACT GGCAAAGGAA 
AATGCAAAGT ATGTTAGCAA AAAATCGGGA ATCATTTCTG CTAAAAAGGC TCTCAATGAT 
CTCCATTTTT GGCTGGGACA GAACTCCTTT TCTCAAGTTT TTGTCGATCA AATGGATGTT 
GATGTGGTAG CGGTTACAAG ACATTCACCG ACCACTCACG AGAGCGTCGT ACTCGTGGCT 
TTTACGGCAT TCAAGCACCC GGATCCCAAT GCTAGCGATT TGAGAAGACA TGTTAGACCT 
TTGAGGGTAG AAGGCGTAGT AGATGAAATT ATTCTTGAAG CATCCCTCGC GCACGGAGAA 
AAAGAAAATG GTATCCCTCC GTTCTCTAAT CCGAAGAAAT ACGTAAAGAA CGAAGACTAC 
ATAAACGGTT ACTCTGAGTA CTTGGTGGAC GTCAAGGAGC ACATTCAAAT CTGTGATTCT 
ACAAAAGTTG AAAAGGTCGA TTCCGGAGAT CCTAAAATAA CGCAATTGAA CTTTATTAAT 
TTCCAGCCCG GATCAATAAT CACTATTCGG GTGTCACTCC ACGCAAACAT AAAGCCAGCT 
CTTGAATCCA TCAATGACCT TATCTCGACC CTCACATCTC CGAACAATTC GGAATTGCGT 
GCAATTGTTT CGCGCATGAA CTTTGCGGAC CTTAACAAAG CACTTTATAG GTGTGATCAA 
GAAGAGCGAG ATACGACTGC CAATAAATTT GGAGTTTATG ATATTCCCGG TTATGGCCCA 
CTAGTTTACG CTGGTTTGCA AGGTGTAATT TCATTGCTTG CTGATATCAG ATCGAACAAC 
GATCTTGGTC ATCCACTATG TGGCAACCTT CGTCAAGGCA ATTGGTTGAT AGATTATACT 
TGGCAACGTT TGAAGGAAGA TGAAGGTACT GCAGCTCTTG GAGAGTGGCT GGAGCAGGCC 
TGCGAACCAT TCAAGCTAAT TCCTCGTTAT TTAGTGCCCA GTTACTTTGA CGTGATCATC 
GTTAACGTGT ATTCTACTCT CTTAGATCAA TGCTTTAATC TCATGTCTAG CTTTGTGAAA 
GATGGAACCG ATTTCACCAG ACTGCTGTCA CTAGTATCTG TGCAAGTTGG CGGTATAATA 
AAATCAGCGC AATTGCCAGA TTTATCACCG AACCTAGATC CACCGAAACC TAGCATACGA 
GAGGTGGATG GCAAATCAGA ACAGGAATGT TTGACCATGT CTGCTGGACT ACCACACTTT 
ACAGTAGGAT ACATGAGAAA CTGGGGACGA GATACGTTTA TCGCTTTGAG AGGTCTGTTC 
TTATTAACCG GCAGACATGC GGAGGCTCGA TTCATCATTC TGGGATACGC CGCTACGCTT 
CGTCACGGTT TGATACCAAA TCTCTTGGAC AGAGGCGTCA ACGCCAGATT CAATTGCCGA 
GATGCCGTTT GGTGGTGGTT GTACACTATT CAATGCTATG TCAAAGAAGT CCCAAATGGC 
TTAAAACTTC TGTCGGACAA AGTATCTAGA TTGTTCCCTA CTGACGATTC GGACGCTCTT 
CCAGCCGGAG AAGTGGATGA AACACTGTCG GATGTCATGC AGGAAGCACT GACAGTTCAC 
TTTCAAGGTC TGTGCTTCCG TGAGCGCAAC GCCGGTAAGC GCATCGACGA GCACATGACT 
GATCGTGGAT TCAACAATCA GATAGGTGTG CATCCAGAGA CGGGCTTTGT ATTTGGCGGT 
AACGAGGCCA ATTGCGGCAC TTGGATGGAC AAGATGGGCT CCTCGGAAAA GGCAGGAAAC 
AAAGGCAAAC CTGCCACTCC CAGAGACGGA TCTGCGGTTG AAATTATTGG GCTCTGTCAC 
AGCACTCTCA ATTTTCTGGC AGAATTGTAC AAACAGAATC TATTTCCATA TGGTAGCGTG 
CAGCGGAAAA ATCGCGATGG CTCAACGATA ACTTGGAGTT ACAAACAGTG GGCGGATAAG 
ATAAAGGAGA GTTTCGATAC TTACTTCTAT GTGAACGAGA AACCTACCGA TGGTGAATTA 
CGACCGGACT TGATTCATCG ACGAGGAATT TACAAAGATA CTCACGGAGC CTCACAACCG 
TGGGCAGATT ATCAATTGCG ACCTAACTTC CCAGTGGCTA TGGTTGCTTC GCCGAACCTA 
TTCAAGCCCG AGCACGCCTG GCTGGCATTA AAGAAGGCCG AAGAAATCCT CCTTGGTCCC 
CTAGGCATGA AAACCCTAGA TCCCGCTGAC TGGGCCTACG ATGGCTTCTA CAACAATGCC 
AACGACTCGA ATGACCCGAA AGTAGCTCAC GGATGGAATT ATCACCAGGG ACCAGAGTGG 
CTATGGCCGA TCGGCTACTT TTTGCGAGCG CGCTTGCACT ACGCGCCCCT CGTTGGTGGC 
GAAGAGGAGT TAAAACGGAC TATCGCCTCG ACCGAAGCTA TCATCTCGAG ACATCTGACC 
GAAGCTTCAA ACAACCACTG GCGTGGACTT CCCGAGTTAA CCAATCGCGA GGGCGAGTAT 
TGCGCTGATA GCTGTCGCAC TCAGGCCTGG AGCGCTTCCG CAATTGTCGA GGTCCTCCAC 
GATTTGGACA AGCTGAAAAA GGATCTGAAG TTTGACGACG AGCAGGTCCT CACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016842161.1
CDS	120..4799
Translation	MENLGFSADTQNDDQVRVLTLNKGEHQDGTLYKLKKGWVVEFRLGASLLGHNLNIFTNHPLLVGNPFERRTYYQLPWNHESAFIRVSLAGSFHFYLAEASGEDAGKVIASGYLLVDPELTVGVNNQPLPLDCIQCQTVLSKLMGPFSTWEEKLRVSRKSGYNMIHFTPIQAIGDSKSAYSLSDQLKLNPSFNDAPNKETSFDDVEKLITKMREEWNTVSICDIVLNHTANESPFLINNPECSYNCNNCPHLRPSYLLDAILFELTIRVSSGEWEHKGIPPVVETEEHLNAIRHALHTYFLPLAKIHELYTVDINEIVSEFLNIARNQIPQDEPPETHNESIVIIRDPEYRRLKSTIDMQLALKIYNIYRPDCFDEDSRLKRCAEELRNKLQELNDAATNEIQNHLNAAVENSVAGIRYFRVQADGPRQKEISERNPLVPRYFTDYGSPRSFVEREDIMYSNSGCYLMAHNGWVMNSDPLKNFADADSHVYIRRELIAWGDSVKLRYGEKPEDCPALWERMTTYVQQMAKIFDGVRLDNCHSTPIPVAEHMLDAARQVRPDLYVAAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFIQARHRPLAPSIAHALFLDQTHDNPSPIEKRSVFDLLPSAALVSMACCASGSNRGYDELVPHHIHVVDEDRQYTSWTDKEGLAKENAKYVSKKSGIISAKKALNDLHFWLGQNSFSQVFVDQMDVDVVAVTRHSPTTHESVVLVAFTAFKHPDPNASDLRRHVRPLRVEGVVDEIILEASLAHGEKENGIPPFSNPKKYVKNEDYINGYSEYLVDVKEHIQICDSTKVEKVDSGDPKITQLNFINFQPGSIITIRVSLHANIKPALESINDLISTLTSPNNSELRAIVSRMNFADLNKALYRCDQEERDTTANKFGVYDIPGYGPLVYAGLQGVISLLADIRSNNDLGHPLCGNLRQGNWLIDYTWQRLKEDEGTAALGEWLEQACEPFKLIPRYLVPSYFDVIIVNVYSTLLDQCFNLMSSFVKDGTDFTRLLSLVSVQVGGIIKSAQLPDLSPNLDPPKPSIREVDGKSEQECLTMSAGLPHFTVGYMRNWGRDTFIALRGLFLLTGRHAEARFIILGYAATLRHGLIPNLLDRGVNARFNCRDAVWWWLYTIQCYVKEVPNGLKLLSDKVSRLFPTDDSDALPAGEVDETLSDVMQEALTVHFQGLCFRERNAGKRIDEHMTDRGFNNQIGVHPETGFVFGGNEANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIIGLCHSTLNFLAELYKQNLFPYGSVQRKNRDGSTITWSYKQWADKIKESFDTYFYVNEKPTDGELRPDLIHRRGIYKDTHGASQPWADYQLRPNFPVAMVASPNLFKPEHAWLALKKAEEILLGPLGMKTLDPADWAYDGFYNNANDSNDPKVAHGWNYHQGPEWLWPIGYFLRARLHYAPLVGGEEELKRTIASTEAIISRHLTEASNNHWRGLPELTNREGEYCADSCRTQAWSASAIVEVLHDLDKLKKDLKFDDEQVLTN

Target ORF information:

RefSeq Version	XM_016986672.1
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis glycogen debranching enzyme (LOC100117168), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016986672.1

ORF Insert Sequence:

ATGGAAAATC TCGGCTTCTC TGCGGATACC CAGAACGACG ACCAAGTGCG AGTACTGACT 
CTGAATAAGG GAGAGCACCA GGATGGGACC CTGTACAAGC TTAAAAAAGG ATGGGTCGTG 
GAGTTCAGAT TAGGGGCATC GCTTCTGGGC CACAATCTCA ACATATTCAC TAATCATCCT 
CTACTCGTCG GCAACCCGTT CGAGAGGCGC ACGTACTATC AGCTGCCATG GAATCACGAG 
TCAGCTTTCA TACGCGTCTC GCTGGCCGGC TCTTTTCATT TTTATCTGGC AGAAGCGAGT 
GGAGAGGACG CCGGAAAAGT GATAGCTTCC GGCTACTTGT TGGTCGATCC GGAGCTAACC 
GTGGGCGTCA ACAACCAGCC GCTGCCGCTA GACTGCATAC AATGCCAGAC GGTCCTCTCA 
AAGCTCATGG GACCCTTCTC AACGTGGGAG GAGAAGCTCA GGGTCTCTCG CAAGTCAGGC 
TACAACATGA TCCACTTCAC ACCGATACAG GCGATAGGAG ACTCAAAATC AGCTTACAGC 
CTCAGCGATC AGCTGAAGCT CAACCCCTCG TTCAATGATG CACCGAACAA AGAGACCAGC 
TTCGACGACG TCGAGAAGTT GATTACAAAA ATGAGAGAAG AGTGGAACAC TGTGAGCATT 
TGCGACATAG TTTTGAATCA CACGGCGAAC GAGAGTCCAT TTTTGATAAA CAATCCCGAA 
TGTAGTTACA ACTGCAACAA CTGTCCACAT CTCCGTCCGT CTTATCTTTT GGATGCCATT 
TTATTCGAGT TGACAATACG AGTGTCGTCC GGAGAATGGG AGCACAAAGG AATTCCTCCC 
GTCGTCGAGA CTGAAGAGCA CTTGAACGCA ATCCGTCATG CTCTGCACAC TTACTTTCTA 
CCTCTGGCGA AAATTCACGA ACTGTATACT GTAGATATCA ACGAGATCGT GTCCGAGTTT 
TTGAACATTG CTCGCAACCA GATACCGCAA GACGAGCCAC CCGAAACCCA TAACGAAAGC 
ATCGTTATCA TAAGGGATCC AGAATATCGC CGCTTAAAGT CCACTATAGA TATGCAGCTT 
GCTTTGAAGA TCTACAATAT TTATAGACCC GATTGTTTTG ACGAGGACTC ACGACTGAAA 
CGCTGCGCGG AAGAACTGAG AAACAAGTTG CAGGAGTTAA ACGATGCCGC AACTAACGAA 
ATCCAAAATC ATCTCAATGC CGCGGTTGAA AACTCGGTAG CCGGCATTCG ATACTTCAGA 
GTCCAAGCAG ATGGGCCAAG ACAGAAAGAG ATAAGCGAAA GAAATCCACT TGTTCCTAGG 
TATTTCACGG ATTACGGAAG TCCTAGATCG TTTGTGGAAA GAGAAGACAT CATGTACTCG 
AATTCTGGAT GTTACTTGAT GGCTCACAAC GGCTGGGTCA TGAACAGCGA TCCCTTGAAA 
AACTTTGCCG ATGCTGACTC TCATGTTTAC ATAAGAAGGG AATTGATTGC GTGGGGCGAT 
AGTGTCAAAT TAAGATATGG CGAAAAACCG GAAGATTGCC CTGCTCTTTG GGAACGTATG 
ACGACATATG TTCAGCAAAT GGCTAAAATC TTCGACGGCG TCAGACTCGA CAATTGTCAC 
TCGACCCCTA TTCCAGTAGC AGAACACATG TTGGATGCTG CTCGTCAAGT AAGACCAGAT 
TTGTACGTTG CCGCTGAACT CTTCACCAAC TCCGATCAGA AAGATAATAT TTTCGTCAAT 
CGTCTTGGAA TCACATCGTT GATAAGAGAG GCCATGTCAG CGTGGGACAG CCATGAGGAA 
GGCCGTCTGG TATACCGCTA TGGTGGCGAG CCCGTAGGTG CGTTTATCCA GGCTCGACAT 
CGTCCTCTGG CACCGAGTAT CGCGCATGCT TTATTCCTTG ATCAGACGCA TGACAACCCG 
AGCCCCATCG AGAAGCGTAG CGTATTTGAC TTATTGCCCA GTGCCGCACT GGTCTCCATG 
GCCTGCTGTG CTAGTGGCAG TAATCGCGGT TACGACGAGC TCGTGCCTCA TCATATTCAC 
GTTGTCGATG AAGACAGACA ATATACTTCT TGGACAGATA AGGAAGGACT GGCAAAGGAA 
AATGCAAAGT ATGTTAGCAA AAAATCGGGA ATCATTTCTG CTAAAAAGGC TCTCAATGAT 
CTCCATTTTT GGCTGGGACA GAACTCCTTT TCTCAAGTTT TTGTCGATCA AATGGATGTT 
GATGTGGTAG CGGTTACAAG ACATTCACCG ACCACTCACG AGAGCGTCGT ACTCGTGGCT 
TTTACGGCAT TCAAGCACCC GGATCCCAAT GCTAGCGATT TGAGAAGACA TGTTAGACCT 
TTGAGGGTAG AAGGCGTAGT AGATGAAATT ATTCTTGAAG CATCCCTCGC GCACGGAGAA 
AAAGAAAATG GTATCCCTCC GTTCTCTAAT CCGAAGAAAT ACGTAAAGAA CGAAGACTAC 
ATAAACGGTT ACTCTGAGTA CTTGGTGGAC GTCAAGGAGC ACATTCAAAT CTGTGATTCT 
ACAAAAGTTG AAAAGGTCGA TTCCGGAGAT CCTAAAATAA CGCAATTGAA CTTTATTAAT 
TTCCAGCCCG GATCAATAAT CACTATTCGG GTGTCACTCC ACGCAAACAT AAAGCCAGCT 
CTTGAATCCA TCAATGACCT TATCTCGACC CTCACATCTC CGAACAATTC GGAATTGCGT 
GCAATTGTTT CGCGCATGAA CTTTGCGGAC CTTAACAAAG CACTTTATAG GTGTGATCAA 
GAAGAGCGAG ATACGACTGC CAATAAATTT GGAGTTTATG ATATTCCCGG TTATGGCCCA 
CTAGTTTACG CTGGTTTGCA AGGTGTAATT TCATTGCTTG CTGATATCAG ATCGAACAAC 
GATCTTGGTC ATCCACTATG TGGCAACCTT CGTCAAGGCA ATTGGTTGAT AGATTATACT 
TGGCAACGTT TGAAGGAAGA TGAAGGTACT GCAGCTCTTG GAGAGTGGCT GGAGCAGGCC 
TGCGAACCAT TCAAGCTAAT TCCTCGTTAT TTAGTGCCCA GTTACTTTGA CGTGATCATC 
GTTAACGTGT ATTCTACTCT CTTAGATCAA TGCTTTAATC TCATGTCTAG CTTTGTGAAA 
GATGGAACCG ATTTCACCAG ACTGCTGTCA CTAGTATCTG TGCAAGTTGG CGGTATAATA 
AAATCAGCGC AATTGCCAGA TTTATCACCG AACCTAGATC CACCGAAACC TAGCATACGA 
GAGGTGGATG GCAAATCAGA ACAGGAATGT TTGACCATGT CTGCTGGACT ACCACACTTT 
ACAGTAGGAT ACATGAGAAA CTGGGGACGA GATACGTTTA TCGCTTTGAG AGGTCTGTTC 
TTATTAACCG GCAGACATGC GGAGGCTCGA TTCATCATTC TGGGATACGC CGCTACGCTT 
CGTCACGGTT TGATACCAAA TCTCTTGGAC AGAGGCGTCA ACGCCAGATT CAATTGCCGA 
GATGCCGTTT GGTGGTGGTT GTACACTATT CAATGCTATG TCAAAGAAGT CCCAAATGGC 
TTAAAACTTC TGTCGGACAA AGTATCTAGA TTGTTCCCTA CTGACGATTC GGACGCTCTT 
CCAGCCGGAG AAGTGGATGA AACACTGTCG GATGTCATGC AGGAAGCACT GACAGTTCAC 
TTTCAAGGTC TGTGCTTCCG TGAGCGCAAC GCCGGTAAGC GCATCGACGA GCACATGACT 
GATCGTGGAT TCAACAATCA GATAGGTGTG CATCCAGAGA CGGGCTTTGT ATTTGGCGGT 
AACGAGGCCA ATTGCGGCAC TTGGATGGAC AAGATGGGCT CCTCGGAAAA GGCAGGAAAC 
AAAGGCAAAC CTGCCACTCC CAGAGACGGA TCTGCGGTTG AAATTATTGG GCTCTGTCAC 
AGCACTCTCA ATTTTCTGGC AGAATTGTAC AAACAGAATC TATTTCCATA TGGTAGCGTG 
CAGCGGAAAA ATCGCGATGG CTCAACGATA ACTTGGAGTT ACAAACAGTG GGCGGATAAG 
ATAAAGGAGA GTTTCGATAC TTACTTCTAT GTGAACGAGA AACCTACCGA TGGTGAATTA 
CGACCGGACT TGATTCATCG ACGAGGAATT TACAAAGATA CTCACGGAGC CTCACAACCG 
TGGGCAGATT ATCAATTGCG ACCTAACTTC CCAGTGGCTA TGGTTGCTTC GCCGAACCTA 
TTCAAGCCCG AGCACGCCTG GCTGGCATTA AAGAAGGCCG AAGAAATCCT CCTTGGTCCC 
CTAGGCATGA AAACCCTAGA TCCCGCTGAC TGGGCCTACG ATGGCTTCTA CAACAATGCC 
AACGACTCGA ATGACCCGAA AGTAGCTCAC GGATGGAATT ATCACCAGGG ACCAGAGTGG 
CTATGGCCGA TCGGCTACTT TTTGCGAGCG CGCTTGCACT ACGCGCCCCT CGTTGGTGGC 
GAAGAGGAGT TAAAACGGAC TATCGCCTCG ACCGAAGCTA TCATCTCGAG ACATCTGACC 
GAAGCTTCAA ACAACCACTG GCGTGGACTT CCCGAGTTAA CCAATCGCGA GGGCGAGTAT 
TGCGCTGATA GCTGTCGCAC TCAGGCCTGG AGCGCTTCCG CAATTGTCGA GGTCCTCCAC 
GATTTGGACA AGCTGAAAAA GGATCTGAAG TTTGACGACG AGCAGGTCCT CACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC100117168 cDNA ORF clone, Nasonia vitripennis(jewel wasp)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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