COL4A4 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL4A4
Entrez Gene ID	100073948
Full Name	collagen type IV alpha 4 chain
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007669913.1	XP_007668103.1	collagen alpha-4(IV) chain
XM_029068967.1	XP_028924800.1	collagen alpha-4(IV) chain isoform X1
XM_029068970.1	XP_028924803.1	collagen alpha-4(IV) chain isoform X3
XM_029068968.1	XP_028924801.1	collagen alpha-4(IV) chain isoform X1
XM_029068969.1	XP_028924802.1	collagen alpha-4(IV) chain isoform X2

Pathways from BioSystems
Gene Ontology

The following COL4A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc132060	XM_029068967.1 Latest version!	Ornithorhynchus anatinus collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc132060	XM_029068968.1 Latest version!	Ornithorhynchus anatinus collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc132061	XM_029068969.1 Latest version!	Ornithorhynchus anatinus collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc132062	XM_029068970.1 Latest version!	Ornithorhynchus anatinus collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc02117	XM_007669913.1 Latest version!	Ornithorhynchus anatinus collagen type IV alpha 4 (COL4A4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL4A4 ( XM_029068967.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028924800.1 Ornithorhynchus anatinus collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOc132060
Clone ID Related Accession (Same CDS sequence)	XM_029068967.1 , XM_029068968.1
Accession Version	XM_029068967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAC TAAGATGTTA TTTCAGAGGG ATTAAAAATA TCTCCAAACC CTGGTGGCTC 
ATAATTATTT TTTCTCTACC GGTTTATGGG GGTGGAAAAA AATACACCGC CATTTGTGGA 
GGACGAAATT GTTCAGCTTG CCAATGTTTT CCTGAGAAAG GATCAAGGGG TCAGCCAGGA 
GAGCTTGGAC CACAGGGACC TATCGGACCA TGTGGAGCTT CAGGACCCCC TGGGTTGCCA 
GGAGAGAAAG GAATGAGAGG GGATGATGGA TCTGCGGGAG CAGCTGGAGA AAGGGGTGAT 
AAGGGCCCAA CAGGAGTTCC TGGCTTTCCA GGTTTGGATG GTGTGCCTGG CTACCCAGGA 
CCTCCCGGAT CAAGAGGTAA ACCGGGTTTA GATGGCCATA ATGGTTCAAG AGGAGATCCA 
GGCTTTCCTG GACAACATGG ATACCCAGGA CAAGTTGGCC CAGCTGGTCT ACCTGGACAA 
AAAGGAGAGA AAGGAATCTC AGCATATGTT GCAGAGAAAG GTCCACCTGG AGATCAAGGT 
ATCCCAGGAC CTCCTGGCTC ACCTGGACCA AAGGGCCCAA GGGGAGCAAA GGGCGGTATG 
GGGTTTCCGG GTAAACCAGG TGCAGATGGT CCTCAAGGCC TTCCTGGATT GCCAGGTGCG 
CAGGGCAATC CAGGAGTTGG CATTAAAGGA CAAACTGGAG ATCCAGGAGA AGTTGGACAT 
CAAGGGCCTC CAGGGCCACC CCTACTGGTT GGGCCTCCAA GTTCATGTCT CCACAAAGGA 
GAAAAAGGCA CAAAAGGGAT GCCTGGAAAA AGTGGCCTCC CTGGCCCAGT TGGAGCTAAG 
GGTGAACCTG GAAGTGGGGT GAAGGGAGAG AAAGGTATTC CTGGATTTCC TGGTCTTCGG 
GGTCATTCTG GTTCCTATGG ATCCCCAGGC TCTCCAGGAT TAAAGGGGGA ACCTGGAGAA 
GTTGGCATTC CTGGGAAGTT TGGATTTCCA GGCATAAAGG GAGATCCTGG AGACCGGGGG 
GAACCAGGGC CACCAGGTAT TATGGTAACT CCACCTGTTC CACTAAAAGG ACTTCCAGGA 
GATCCAGGAG TTCCAGGTCC CTATGGAGAA ATGGGATCAG TTGGAAAGGT TGGCCCACCT 
GGTCCTTCAG GTAAACCAGG TGAAGACCGG GCAGTAGGAG TGACAGGGCC AATGGGATCC 
CGAGGAGCAC CGGGTGTTCC AGGTCCCCCA GGGGAAGTTG GTGTTCCTGG AAAAGCAGAT 
GCCCCACCAG GAAAGCCTGG GAATCCAGGA TACCCAGGCA CACCAGGAGC ACGAGGAGAG 
CAGGGGCTGC CTGGACCAGA CGTTGTGCAC TGTACTTCTG GACCTCCTGG ACCTCAAGGA 
ATGAAAGGAA AAGCAGGACC TCCAGGAAGA AGTGGTGTGA AAGGTGAAAA AGGAAATGAA 
GGTGCCTGTG CCTGTACCCC TGGTGTCATT GGCCTACCAG GGACTCCAGG GCTCCCTGGA 
AGAAAAGGAA GGAAAGGGGA GCTGGGATAT CCTGGGAGAT TTGGAGAAAA AGGTGACCAA 
GGTTCTCCCG GGGCAAAAGG ATATCCAGGA CCTTCTGGTA TACCTGGTTC CTTGGGAATG 
ACTGGACCAA AAGGAGAAAA GGGAGACAGA GTTATCCCCA GAATAAAAGG AAGAAAAGGA 
GAAAGAGGCT CCCAAGGACC GCCTGGATTT CCAGGACTTC AAGGACAACC AGGGCAGGAT 
GGAACTGAAG GACCAGTAGG ATTACCTGGA TTGCCAGGTG ATCGAGGAGG CGCAGTTCCA 
GGTGCTAAGG GATTTCCCGG ACACCAAGGA GCCCCTGGGA GGACTGGACC TAGGGGTCCT 
TCGGGTTTGG GGTTTCCTGG ACGACAGGGC ATGACAGGCA TCCCAGGAGA CCGAGGTACC 
GTTGGTGACA GGGGACCACC TGGTGCAAAG GGCCAGAAAG GTGACACATT TCCCTGTGCC 
TCAGATTTTC CTGGAAATCC AGGCCCACCA GGCTTTCAAG GTCCTCCAGG TCCAAAGGGA 
ATTCAAGGAC CCCCAGGTCC AGCTGGACCC CATGGTTTTG ACGGGGCCAA AGGCCAGAGA 
GGCAAGCCAG GAATTTCCGA AGTGCCAGGA CCAGCTGGCT TTCCTGGTGA CAATGGAGAT 
CCAGGTTTTA AAGGTGACAA AGGAACCTCA CCAGTTGGTC CTCAGGGTTT TCCTGGTTCA 
CCTGGTATCA ATGGGCAAAA AGGTATGCCA GGTGATCCTG CTTTTGGCCA CCCAGGACTT 
CCAGGAAACA AAGGTTCTGC AGGATTTCCC GGACCTAAAG GATTTAGAGG TGACCTAGGA 
TATCCAGGAT CTCCAGGACA GGCTGGTACC CCAGGGCTTC CAGGAACCAA AGGATTTAAA 
GGCCAAAAAG GGCATAATGG ACCTCCGGGT TCTCCAGGTC CACCTGGATA CTTATGTGAA 
AAAGGCTCAC CTGGGTTGCC AGGACAACCA GGGCTCCCTG GACCACAAGG TAATCCAGGT 
TACCCTGGCT TGAAAGGCCA GCAAGGAGAT ATGGGTGCCC CTGGACCAGC TGGAATGAAG 
GGTCTTCCAG GAGCACCAGG ATTAAATGGA TCATCTGGTC CCCCAGGACC ACCAGGAAAT 
CGAGGGCATT TGGGGGATGA TGGGTTACCT GGATTTCGGG GCCCAAAGGG TGCCCAAGGG 
CTGCCGGGTA TCCCAGGATT CCCAGGCGAG CTAGGAAAGA AAGGGCAAGC AGGACACCCT 
GGGAGAAAGG GTGAACGGGG AGGAGATGGT CATCCTGGCC ACCCAGGAGA CAAAGGGGAC 
TGGGGACCTA AAGGTGAAAA AGGACTTCCT GGGGATGAAG GTAGTATTTC CATGATTTCC 
ACAAAGGGAG AAAAGGGGGA ATTAGGTCAA CCTGGCGAAT ATGGAGTCCC AGGAGAAAGA 
GGAGATAAAG GTTCTTCTGG ACTCCAAGGG GAAGAAGGAT TACCAGGAAG AGATGGACTT 
CCAGGGTTTC AGAAAGGTCA TCCTGGTCCA AGTGGCCAGC CTGGATTTCC AGGACTGCCT 
GGCTTCCCAG GCTCTCCAGG CTCAAGAGGA GTATTTGGTT TCCCTGGACT ACCGGGTGAT 
CCGGGAGATG ATGGTTCACG GGGCATACCT GGACCTTCAG GACCTATTGG AGTCAAAGGC 
CCCAAAGGAA AAAGAGGGGA TTCAATCAGT TTACTTGGCC CACTTGGAGA AAAAGGAAGA 
CCTGGAAACC CTGGATGTGC AGGTCATTTT GGAGCCCCAG GTGACCTGGG TTTGCCTGGG 
CCTCCGGGGT ATCCTGGAAC CCCAGGAAGG CCAGGACCAC CAGGATACCC AGGACAACCT 
GGCTTTCCAG GTACTCAGGG GACCCCTGGA CAAAGAGGAA TTCCAGGAGA AATAGGCGAC 
CCTGGGATCT CAGGACCTAT TGGAGATCAA GGACTTGCTG GGCCCCGAGG CATGAAAGGG 
TTCTCAGGAA TTCCAGGATT AAATGGTTTG CATGGACTCA AGGGGCCAAA AGGAGCGAAG 
GGTTCACCAG GTTTCATCGA AAGAGGACCA CCAGGTTTTG CTGGATTGCC TGGAGCAAAA 
GGGGAAAAAG GAGAACCTGG AAGGGTGGGA CTTTCTTTTC CAGGTCCTTT TGGTGAAAGA 
GGACCTCAAG GTCCCCCAGG GAGGCCTGGA CTTCAGGGAT CTGCAGGTCC TAAAGGAAGA 
ACACCTGAAG GAGAAATTTC TTATCCTGGT CCTCAAGGAG ATATAGGATT TCATGGCCCC 
GATGGTATAC GAGGTCCACC AGGGTCTCCA GGTCAACCTG GAGGTGTTGA CTTTTGGAAA 
GGGAGTCCAG GTGATGATGG CTTTCCAGGA CCACCAGGTC CCTCTGGTCC ACCAGGACAT 
CCTGGACCAA AAGGCATCCC AGGATGCGAA GGAAGAGATG GCCAAAAAGG GCCAATGGGA 
CATCCTGGTC TTCAAGGTCC ACCTGGACGC CCTGGGCCAT ATGGAGATAA AGGGAGTCCA 
GGACCCCCCG GGACTCCTGG GTCACCTGGT CCCAGAGGGG ACCCTGGCCT ACCTGGAGAT 
ATTGCAGATT GTCCCAAAAT CCCCGGGCCC CCAGGGATTC CAGGTTCAAG AGGACCAGAA 
GGACGCATGG GATTTCCCGG TCCAAGAGGA CCCCCAGGCT TACCAGGACT TAAAGGTGAA 
CCAGGGCCAG ATGGCCAGAG AGGTCAAGAT GGCTTTCCTG GATCGCCAGG TGCACCTGGA 
CAAAAGGGAG ACTCAGGGGA ACCAGGCTGT GATGGGCCAC CAGGTCCACC TGGCCCAACT 
GGAGATCCAG GATCCAGAGG GCCAAGAACT GAATACCTTA ATGGATTCCT CCTGGTTCTT 
CATAGTCAGA CAGACAAAAT ACCTTCTTGT CCTGATGGAT TGCCCAAGTT GTGGACTGGG 
TACAGTCTGC TTTATATTGA AGGCCAGGAG AAAGCACATA ATCAAGATCT GGGTCTCGCG 
GGATCCTGTC TTCCCCTGTT CAGCACGATG CCATTTGCTT ACTGCAACAT CAATCACGTC 
TGTTTCTATG CCAGGAGAAA TGATAAATCC TACTGGCTAT CCAGTGCCGC TCCTCTACCC 
ATGATGCCCC TCTCTGAAGA TAAAATACAG CCGTACATTA GCCGGTGTGT GGTATGCGAG 
TCCCCGGCCC AGGCCTTGGC CGTTCACAGC CAAGATGAAT CCATCCCTCC ATGTCCGACA 
TCATGGAGAA GCCTCTGGAT AGGTTATTCT TTCCTAATGC ACACAGGAGC TGGAGATCAA 
GGAGGAGGAC AGTCCCTTGT GTCCCCTGGA AGCTGCCTTG AAGACTTCCG AGCAGCCCCC 
TTCATTGAAT GCCAAGGACG GCAGGGAACG TGTCATTTTT TTGCCAATGA ATACAGCTTC 
TGGCTAACGA CAGTGAAACC AGAATTACAG TTTTCATCAG CTCCCTCACC AAGCACCTTA 
AAAGATGGAC AGGCACAGCG CCAAAAAATC AGCAGATGTC AGGTCTGCAT GAAATACAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028924800.1
CDS	222..5264
Translation	MALLRCYFRGIKNISKPWWLIIIFSLPVYGGGKKYTAICGGRNCSACQCFPEKGSRGQPGELGPQGPIGPCGASGPPGLPGEKGMRGDDGSAGAAGERGDKGPTGVPGFPGLDGVPGYPGPPGSRGKPGLDGHNGSRGDPGFPGQHGYPGQVGPAGLPGQKGEKGISAYVAEKGPPGDQGIPGPPGSPGPKGPRGAKGGMGFPGKPGADGPQGLPGLPGAQGNPGVGIKGQTGDPGEVGHQGPPGPPLLVGPPSSCLHKGEKGTKGMPGKSGLPGPVGAKGEPGSGVKGEKGIPGFPGLRGHSGSYGSPGSPGLKGEPGEVGIPGKFGFPGIKGDPGDRGEPGPPGIMVTPPVPLKGLPGDPGVPGPYGEMGSVGKVGPPGPSGKPGEDRAVGVTGPMGSRGAPGVPGPPGEVGVPGKADAPPGKPGNPGYPGTPGARGEQGLPGPDVVHCTSGPPGPQGMKGKAGPPGRSGVKGEKGNEGACACTPGVIGLPGTPGLPGRKGRKGELGYPGRFGEKGDQGSPGAKGYPGPSGIPGSLGMTGPKGEKGDRVIPRIKGRKGERGSQGPPGFPGLQGQPGQDGTEGPVGLPGLPGDRGGAVPGAKGFPGHQGAPGRTGPRGPSGLGFPGRQGMTGIPGDRGTVGDRGPPGAKGQKGDTFPCASDFPGNPGPPGFQGPPGPKGIQGPPGPAGPHGFDGAKGQRGKPGISEVPGPAGFPGDNGDPGFKGDKGTSPVGPQGFPGSPGINGQKGMPGDPAFGHPGLPGNKGSAGFPGPKGFRGDLGYPGSPGQAGTPGLPGTKGFKGQKGHNGPPGSPGPPGYLCEKGSPGLPGQPGLPGPQGNPGYPGLKGQQGDMGAPGPAGMKGLPGAPGLNGSSGPPGPPGNRGHLGDDGLPGFRGPKGAQGLPGIPGFPGELGKKGQAGHPGRKGERGGDGHPGHPGDKGDWGPKGEKGLPGDEGSISMISTKGEKGELGQPGEYGVPGERGDKGSSGLQGEEGLPGRDGLPGFQKGHPGPSGQPGFPGLPGFPGSPGSRGVFGFPGLPGDPGDDGSRGIPGPSGPIGVKGPKGKRGDSISLLGPLGEKGRPGNPGCAGHFGAPGDLGLPGPPGYPGTPGRPGPPGYPGQPGFPGTQGTPGQRGIPGEIGDPGISGPIGDQGLAGPRGMKGFSGIPGLNGLHGLKGPKGAKGSPGFIERGPPGFAGLPGAKGEKGEPGRVGLSFPGPFGERGPQGPPGRPGLQGSAGPKGRTPEGEISYPGPQGDIGFHGPDGIRGPPGSPGQPGGVDFWKGSPGDDGFPGPPGPSGPPGHPGPKGIPGCEGRDGQKGPMGHPGLQGPPGRPGPYGDKGSPGPPGTPGSPGPRGDPGLPGDIADCPKIPGPPGIPGSRGPEGRMGFPGPRGPPGLPGLKGEPGPDGQRGQDGFPGSPGAPGQKGDSGEPGCDGPPGPPGPTGDPGSRGPRTEYLNGFLLVLHSQTDKIPSCPDGLPKLWTGYSLLYIEGQEKAHNQDLGLAGSCLPLFSTMPFAYCNINHVCFYARRNDKSYWLSSAAPLPMMPLSEDKIQPYISRCVVCESPAQALAVHSQDESIPPCPTSWRSLWIGYSFLMHTGAGDQGGGQSLVSPGSCLEDFRAAPFIECQGRQGTCHFFANEYSFWLTTVKPELQFSSAPSPSTLKDGQAQRQKISRCQVCMKYS

Target ORF information:

RefSeq Version	XM_029068967.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029068967.1

ORF Insert Sequence:

ATGGCGCTAC TAAGATGTTA TTTCAGAGGG ATTAAAAATA TCTCCAAACC CTGGTGGCTC 
ATAATTATTT TTTCTCTACC GGTTTATGGG GGTGGAAAAA AATACACCGC CATTTGTGGA 
GGACGAAATT GTTCAGCTTG CCAATGTTTT CCTGAGAAAG GATCAAGGGG TCAGCCAGGA 
GAGCTTGGAC CACAGGGACC TATCGGACCA TGTGGAGCTT CAGGACCCCC TGGGTTGCCA 
GGAGAGAAAG GAATGAGAGG GGATGATGGA TCTGCGGGAG CAGCTGGAGA AAGGGGTGAT 
AAGGGCCCAA CAGGAGTTCC TGGCTTTCCA GGTTTGGATG GTGTGCCTGG CTACCCAGGA 
CCTCCCGGAT CAAGAGGTAA ACCGGGTTTA GATGGCCATA ATGGTTCAAG AGGAGATCCA 
GGCTTTCCTG GACAACATGG ATACCCAGGA CAAGTTGGCC CAGCTGGTCT ACCTGGACAA 
AAAGGAGAGA AAGGAATCTC AGCATATGTT GCAGAGAAAG GTCCACCTGG AGATCAAGGT 
ATCCCAGGAC CTCCTGGCTC ACCTGGACCA AAGGGCCCAA GGGGAGCAAA GGGCGGTATG 
GGGTTTCCGG GTAAACCAGG TGCAGATGGT CCTCAAGGCC TTCCTGGATT GCCAGGTGCG 
CAGGGCAATC CAGGAGTTGG CATTAAAGGA CAAACTGGAG ATCCAGGAGA AGTTGGACAT 
CAAGGGCCTC CAGGGCCACC CCTACTGGTT GGGCCTCCAA GTTCATGTCT CCACAAAGGA 
GAAAAAGGCA CAAAAGGGAT GCCTGGAAAA AGTGGCCTCC CTGGCCCAGT TGGAGCTAAG 
GGTGAACCTG GAAGTGGGGT GAAGGGAGAG AAAGGTATTC CTGGATTTCC TGGTCTTCGG 
GGTCATTCTG GTTCCTATGG ATCCCCAGGC TCTCCAGGAT TAAAGGGGGA ACCTGGAGAA 
GTTGGCATTC CTGGGAAGTT TGGATTTCCA GGCATAAAGG GAGATCCTGG AGACCGGGGG 
GAACCAGGGC CACCAGGTAT TATGGTAACT CCACCTGTTC CACTAAAAGG ACTTCCAGGA 
GATCCAGGAG TTCCAGGTCC CTATGGAGAA ATGGGATCAG TTGGAAAGGT TGGCCCACCT 
GGTCCTTCAG GTAAACCAGG TGAAGACCGG GCAGTAGGAG TGACAGGGCC AATGGGATCC 
CGAGGAGCAC CGGGTGTTCC AGGTCCCCCA GGGGAAGTTG GTGTTCCTGG AAAAGCAGAT 
GCCCCACCAG GAAAGCCTGG GAATCCAGGA TACCCAGGCA CACCAGGAGC ACGAGGAGAG 
CAGGGGCTGC CTGGACCAGA CGTTGTGCAC TGTACTTCTG GACCTCCTGG ACCTCAAGGA 
ATGAAAGGAA AAGCAGGACC TCCAGGAAGA AGTGGTGTGA AAGGTGAAAA AGGAAATGAA 
GGTGCCTGTG CCTGTACCCC TGGTGTCATT GGCCTACCAG GGACTCCAGG GCTCCCTGGA 
AGAAAAGGAA GGAAAGGGGA GCTGGGATAT CCTGGGAGAT TTGGAGAAAA AGGTGACCAA 
GGTTCTCCCG GGGCAAAAGG ATATCCAGGA CCTTCTGGTA TACCTGGTTC CTTGGGAATG 
ACTGGACCAA AAGGAGAAAA GGGAGACAGA GTTATCCCCA GAATAAAAGG AAGAAAAGGA 
GAAAGAGGCT CCCAAGGACC GCCTGGATTT CCAGGACTTC AAGGACAACC AGGGCAGGAT 
GGAACTGAAG GACCAGTAGG ATTACCTGGA TTGCCAGGTG ATCGAGGAGG CGCAGTTCCA 
GGTGCTAAGG GATTTCCCGG ACACCAAGGA GCCCCTGGGA GGACTGGACC TAGGGGTCCT 
TCGGGTTTGG GGTTTCCTGG ACGACAGGGC ATGACAGGCA TCCCAGGAGA CCGAGGTACC 
GTTGGTGACA GGGGACCACC TGGTGCAAAG GGCCAGAAAG GTGACACATT TCCCTGTGCC 
TCAGATTTTC CTGGAAATCC AGGCCCACCA GGCTTTCAAG GTCCTCCAGG TCCAAAGGGA 
ATTCAAGGAC CCCCAGGTCC AGCTGGACCC CATGGTTTTG ACGGGGCCAA AGGCCAGAGA 
GGCAAGCCAG GAATTTCCGA AGTGCCAGGA CCAGCTGGCT TTCCTGGTGA CAATGGAGAT 
CCAGGTTTTA AAGGTGACAA AGGAACCTCA CCAGTTGGTC CTCAGGGTTT TCCTGGTTCA 
CCTGGTATCA ATGGGCAAAA AGGTATGCCA GGTGATCCTG CTTTTGGCCA CCCAGGACTT 
CCAGGAAACA AAGGTTCTGC AGGATTTCCC GGACCTAAAG GATTTAGAGG TGACCTAGGA 
TATCCAGGAT CTCCAGGACA GGCTGGTACC CCAGGGCTTC CAGGAACCAA AGGATTTAAA 
GGCCAAAAAG GGCATAATGG ACCTCCGGGT TCTCCAGGTC CACCTGGATA CTTATGTGAA 
AAAGGCTCAC CTGGGTTGCC AGGACAACCA GGGCTCCCTG GACCACAAGG TAATCCAGGT 
TACCCTGGCT TGAAAGGCCA GCAAGGAGAT ATGGGTGCCC CTGGACCAGC TGGAATGAAG 
GGTCTTCCAG GAGCACCAGG ATTAAATGGA TCATCTGGTC CCCCAGGACC ACCAGGAAAT 
CGAGGGCATT TGGGGGATGA TGGGTTACCT GGATTTCGGG GCCCAAAGGG TGCCCAAGGG 
CTGCCGGGTA TCCCAGGATT CCCAGGCGAG CTAGGAAAGA AAGGGCAAGC AGGACACCCT 
GGGAGAAAGG GTGAACGGGG AGGAGATGGT CATCCTGGCC ACCCAGGAGA CAAAGGGGAC 
TGGGGACCTA AAGGTGAAAA AGGACTTCCT GGGGATGAAG GTAGTATTTC CATGATTTCC 
ACAAAGGGAG AAAAGGGGGA ATTAGGTCAA CCTGGCGAAT ATGGAGTCCC AGGAGAAAGA 
GGAGATAAAG GTTCTTCTGG ACTCCAAGGG GAAGAAGGAT TACCAGGAAG AGATGGACTT 
CCAGGGTTTC AGAAAGGTCA TCCTGGTCCA AGTGGCCAGC CTGGATTTCC AGGACTGCCT 
GGCTTCCCAG GCTCTCCAGG CTCAAGAGGA GTATTTGGTT TCCCTGGACT ACCGGGTGAT 
CCGGGAGATG ATGGTTCACG GGGCATACCT GGACCTTCAG GACCTATTGG AGTCAAAGGC 
CCCAAAGGAA AAAGAGGGGA TTCAATCAGT TTACTTGGCC CACTTGGAGA AAAAGGAAGA 
CCTGGAAACC CTGGATGTGC AGGTCATTTT GGAGCCCCAG GTGACCTGGG TTTGCCTGGG 
CCTCCGGGGT ATCCTGGAAC CCCAGGAAGG CCAGGACCAC CAGGATACCC AGGACAACCT 
GGCTTTCCAG GTACTCAGGG GACCCCTGGA CAAAGAGGAA TTCCAGGAGA AATAGGCGAC 
CCTGGGATCT CAGGACCTAT TGGAGATCAA GGACTTGCTG GGCCCCGAGG CATGAAAGGG 
TTCTCAGGAA TTCCAGGATT AAATGGTTTG CATGGACTCA AGGGGCCAAA AGGAGCGAAG 
GGTTCACCAG GTTTCATCGA AAGAGGACCA CCAGGTTTTG CTGGATTGCC TGGAGCAAAA 
GGGGAAAAAG GAGAACCTGG AAGGGTGGGA CTTTCTTTTC CAGGTCCTTT TGGTGAAAGA 
GGACCTCAAG GTCCCCCAGG GAGGCCTGGA CTTCAGGGAT CTGCAGGTCC TAAAGGAAGA 
ACACCTGAAG GAGAAATTTC TTATCCTGGT CCTCAAGGAG ATATAGGATT TCATGGCCCC 
GATGGTATAC GAGGTCCACC AGGGTCTCCA GGTCAACCTG GAGGTGTTGA CTTTTGGAAA 
GGGAGTCCAG GTGATGATGG CTTTCCAGGA CCACCAGGTC CCTCTGGTCC ACCAGGACAT 
CCTGGACCAA AAGGCATCCC AGGATGCGAA GGAAGAGATG GCCAAAAAGG GCCAATGGGA 
CATCCTGGTC TTCAAGGTCC ACCTGGACGC CCTGGGCCAT ATGGAGATAA AGGGAGTCCA 
GGACCCCCCG GGACTCCTGG GTCACCTGGT CCCAGAGGGG ACCCTGGCCT ACCTGGAGAT 
ATTGCAGATT GTCCCAAAAT CCCCGGGCCC CCAGGGATTC CAGGTTCAAG AGGACCAGAA 
GGACGCATGG GATTTCCCGG TCCAAGAGGA CCCCCAGGCT TACCAGGACT TAAAGGTGAA 
CCAGGGCCAG ATGGCCAGAG AGGTCAAGAT GGCTTTCCTG GATCGCCAGG TGCACCTGGA 
CAAAAGGGAG ACTCAGGGGA ACCAGGCTGT GATGGGCCAC CAGGTCCACC TGGCCCAACT 
GGAGATCCAG GATCCAGAGG GCCAAGAACT GAATACCTTA ATGGATTCCT CCTGGTTCTT 
CATAGTCAGA CAGACAAAAT ACCTTCTTGT CCTGATGGAT TGCCCAAGTT GTGGACTGGG 
TACAGTCTGC TTTATATTGA AGGCCAGGAG AAAGCACATA ATCAAGATCT GGGTCTCGCG 
GGATCCTGTC TTCCCCTGTT CAGCACGATG CCATTTGCTT ACTGCAACAT CAATCACGTC 
TGTTTCTATG CCAGGAGAAA TGATAAATCC TACTGGCTAT CCAGTGCCGC TCCTCTACCC 
ATGATGCCCC TCTCTGAAGA TAAAATACAG CCGTACATTA GCCGGTGTGT GGTATGCGAG 
TCCCCGGCCC AGGCCTTGGC CGTTCACAGC CAAGATGAAT CCATCCCTCC ATGTCCGACA 
TCATGGAGAA GCCTCTGGAT AGGTTATTCT TTCCTAATGC ACACAGGAGC TGGAGATCAA 
GGAGGAGGAC AGTCCCTTGT GTCCCCTGGA AGCTGCCTTG AAGACTTCCG AGCAGCCCCC 
TTCATTGAAT GCCAAGGACG GCAGGGAACG TGTCATTTTT TTGCCAATGA ATACAGCTTC 
TGGCTAACGA CAGTGAAACC AGAATTACAG TTTTCATCAG CTCCCTCACC AAGCACCTTA 
AAAGATGGAC AGGCACAGCG CCAAAAAATC AGCAGATGTC AGGTCTGCAT GAAATACAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_029068968.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028924801.1 Ornithorhynchus anatinus collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOc132060
Clone ID Related Accession (Same CDS sequence)	XM_029068967.1 , XM_029068968.1
Accession Version	XM_029068968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAC TAAGATGTTA TTTCAGAGGG ATTAAAAATA TCTCCAAACC CTGGTGGCTC 
ATAATTATTT TTTCTCTACC GGTTTATGGG GGTGGAAAAA AATACACCGC CATTTGTGGA 
GGACGAAATT GTTCAGCTTG CCAATGTTTT CCTGAGAAAG GATCAAGGGG TCAGCCAGGA 
GAGCTTGGAC CACAGGGACC TATCGGACCA TGTGGAGCTT CAGGACCCCC TGGGTTGCCA 
GGAGAGAAAG GAATGAGAGG GGATGATGGA TCTGCGGGAG CAGCTGGAGA AAGGGGTGAT 
AAGGGCCCAA CAGGAGTTCC TGGCTTTCCA GGTTTGGATG GTGTGCCTGG CTACCCAGGA 
CCTCCCGGAT CAAGAGGTAA ACCGGGTTTA GATGGCCATA ATGGTTCAAG AGGAGATCCA 
GGCTTTCCTG GACAACATGG ATACCCAGGA CAAGTTGGCC CAGCTGGTCT ACCTGGACAA 
AAAGGAGAGA AAGGAATCTC AGCATATGTT GCAGAGAAAG GTCCACCTGG AGATCAAGGT 
ATCCCAGGAC CTCCTGGCTC ACCTGGACCA AAGGGCCCAA GGGGAGCAAA GGGCGGTATG 
GGGTTTCCGG GTAAACCAGG TGCAGATGGT CCTCAAGGCC TTCCTGGATT GCCAGGTGCG 
CAGGGCAATC CAGGAGTTGG CATTAAAGGA CAAACTGGAG ATCCAGGAGA AGTTGGACAT 
CAAGGGCCTC CAGGGCCACC CCTACTGGTT GGGCCTCCAA GTTCATGTCT CCACAAAGGA 
GAAAAAGGCA CAAAAGGGAT GCCTGGAAAA AGTGGCCTCC CTGGCCCAGT TGGAGCTAAG 
GGTGAACCTG GAAGTGGGGT GAAGGGAGAG AAAGGTATTC CTGGATTTCC TGGTCTTCGG 
GGTCATTCTG GTTCCTATGG ATCCCCAGGC TCTCCAGGAT TAAAGGGGGA ACCTGGAGAA 
GTTGGCATTC CTGGGAAGTT TGGATTTCCA GGCATAAAGG GAGATCCTGG AGACCGGGGG 
GAACCAGGGC CACCAGGTAT TATGGTAACT CCACCTGTTC CACTAAAAGG ACTTCCAGGA 
GATCCAGGAG TTCCAGGTCC CTATGGAGAA ATGGGATCAG TTGGAAAGGT TGGCCCACCT 
GGTCCTTCAG GTAAACCAGG TGAAGACCGG GCAGTAGGAG TGACAGGGCC AATGGGATCC 
CGAGGAGCAC CGGGTGTTCC AGGTCCCCCA GGGGAAGTTG GTGTTCCTGG AAAAGCAGAT 
GCCCCACCAG GAAAGCCTGG GAATCCAGGA TACCCAGGCA CACCAGGAGC ACGAGGAGAG 
CAGGGGCTGC CTGGACCAGA CGTTGTGCAC TGTACTTCTG GACCTCCTGG ACCTCAAGGA 
ATGAAAGGAA AAGCAGGACC TCCAGGAAGA AGTGGTGTGA AAGGTGAAAA AGGAAATGAA 
GGTGCCTGTG CCTGTACCCC TGGTGTCATT GGCCTACCAG GGACTCCAGG GCTCCCTGGA 
AGAAAAGGAA GGAAAGGGGA GCTGGGATAT CCTGGGAGAT TTGGAGAAAA AGGTGACCAA 
GGTTCTCCCG GGGCAAAAGG ATATCCAGGA CCTTCTGGTA TACCTGGTTC CTTGGGAATG 
ACTGGACCAA AAGGAGAAAA GGGAGACAGA GTTATCCCCA GAATAAAAGG AAGAAAAGGA 
GAAAGAGGCT CCCAAGGACC GCCTGGATTT CCAGGACTTC AAGGACAACC AGGGCAGGAT 
GGAACTGAAG GACCAGTAGG ATTACCTGGA TTGCCAGGTG ATCGAGGAGG CGCAGTTCCA 
GGTGCTAAGG GATTTCCCGG ACACCAAGGA GCCCCTGGGA GGACTGGACC TAGGGGTCCT 
TCGGGTTTGG GGTTTCCTGG ACGACAGGGC ATGACAGGCA TCCCAGGAGA CCGAGGTACC 
GTTGGTGACA GGGGACCACC TGGTGCAAAG GGCCAGAAAG GTGACACATT TCCCTGTGCC 
TCAGATTTTC CTGGAAATCC AGGCCCACCA GGCTTTCAAG GTCCTCCAGG TCCAAAGGGA 
ATTCAAGGAC CCCCAGGTCC AGCTGGACCC CATGGTTTTG ACGGGGCCAA AGGCCAGAGA 
GGCAAGCCAG GAATTTCCGA AGTGCCAGGA CCAGCTGGCT TTCCTGGTGA CAATGGAGAT 
CCAGGTTTTA AAGGTGACAA AGGAACCTCA CCAGTTGGTC CTCAGGGTTT TCCTGGTTCA 
CCTGGTATCA ATGGGCAAAA AGGTATGCCA GGTGATCCTG CTTTTGGCCA CCCAGGACTT 
CCAGGAAACA AAGGTTCTGC AGGATTTCCC GGACCTAAAG GATTTAGAGG TGACCTAGGA 
TATCCAGGAT CTCCAGGACA GGCTGGTACC CCAGGGCTTC CAGGAACCAA AGGATTTAAA 
GGCCAAAAAG GGCATAATGG ACCTCCGGGT TCTCCAGGTC CACCTGGATA CTTATGTGAA 
AAAGGCTCAC CTGGGTTGCC AGGACAACCA GGGCTCCCTG GACCACAAGG TAATCCAGGT 
TACCCTGGCT TGAAAGGCCA GCAAGGAGAT ATGGGTGCCC CTGGACCAGC TGGAATGAAG 
GGTCTTCCAG GAGCACCAGG ATTAAATGGA TCATCTGGTC CCCCAGGACC ACCAGGAAAT 
CGAGGGCATT TGGGGGATGA TGGGTTACCT GGATTTCGGG GCCCAAAGGG TGCCCAAGGG 
CTGCCGGGTA TCCCAGGATT CCCAGGCGAG CTAGGAAAGA AAGGGCAAGC AGGACACCCT 
GGGAGAAAGG GTGAACGGGG AGGAGATGGT CATCCTGGCC ACCCAGGAGA CAAAGGGGAC 
TGGGGACCTA AAGGTGAAAA AGGACTTCCT GGGGATGAAG GTAGTATTTC CATGATTTCC 
ACAAAGGGAG AAAAGGGGGA ATTAGGTCAA CCTGGCGAAT ATGGAGTCCC AGGAGAAAGA 
GGAGATAAAG GTTCTTCTGG ACTCCAAGGG GAAGAAGGAT TACCAGGAAG AGATGGACTT 
CCAGGGTTTC AGAAAGGTCA TCCTGGTCCA AGTGGCCAGC CTGGATTTCC AGGACTGCCT 
GGCTTCCCAG GCTCTCCAGG CTCAAGAGGA GTATTTGGTT TCCCTGGACT ACCGGGTGAT 
CCGGGAGATG ATGGTTCACG GGGCATACCT GGACCTTCAG GACCTATTGG AGTCAAAGGC 
CCCAAAGGAA AAAGAGGGGA TTCAATCAGT TTACTTGGCC CACTTGGAGA AAAAGGAAGA 
CCTGGAAACC CTGGATGTGC AGGTCATTTT GGAGCCCCAG GTGACCTGGG TTTGCCTGGG 
CCTCCGGGGT ATCCTGGAAC CCCAGGAAGG CCAGGACCAC CAGGATACCC AGGACAACCT 
GGCTTTCCAG GTACTCAGGG GACCCCTGGA CAAAGAGGAA TTCCAGGAGA AATAGGCGAC 
CCTGGGATCT CAGGACCTAT TGGAGATCAA GGACTTGCTG GGCCCCGAGG CATGAAAGGG 
TTCTCAGGAA TTCCAGGATT AAATGGTTTG CATGGACTCA AGGGGCCAAA AGGAGCGAAG 
GGTTCACCAG GTTTCATCGA AAGAGGACCA CCAGGTTTTG CTGGATTGCC TGGAGCAAAA 
GGGGAAAAAG GAGAACCTGG AAGGGTGGGA CTTTCTTTTC CAGGTCCTTT TGGTGAAAGA 
GGACCTCAAG GTCCCCCAGG GAGGCCTGGA CTTCAGGGAT CTGCAGGTCC TAAAGGAAGA 
ACACCTGAAG GAGAAATTTC TTATCCTGGT CCTCAAGGAG ATATAGGATT TCATGGCCCC 
GATGGTATAC GAGGTCCACC AGGGTCTCCA GGTCAACCTG GAGGTGTTGA CTTTTGGAAA 
GGGAGTCCAG GTGATGATGG CTTTCCAGGA CCACCAGGTC CCTCTGGTCC ACCAGGACAT 
CCTGGACCAA AAGGCATCCC AGGATGCGAA GGAAGAGATG GCCAAAAAGG GCCAATGGGA 
CATCCTGGTC TTCAAGGTCC ACCTGGACGC CCTGGGCCAT ATGGAGATAA AGGGAGTCCA 
GGACCCCCCG GGACTCCTGG GTCACCTGGT CCCAGAGGGG ACCCTGGCCT ACCTGGAGAT 
ATTGCAGATT GTCCCAAAAT CCCCGGGCCC CCAGGGATTC CAGGTTCAAG AGGACCAGAA 
GGACGCATGG GATTTCCCGG TCCAAGAGGA CCCCCAGGCT TACCAGGACT TAAAGGTGAA 
CCAGGGCCAG ATGGCCAGAG AGGTCAAGAT GGCTTTCCTG GATCGCCAGG TGCACCTGGA 
CAAAAGGGAG ACTCAGGGGA ACCAGGCTGT GATGGGCCAC CAGGTCCACC TGGCCCAACT 
GGAGATCCAG GATCCAGAGG GCCAAGAACT GAATACCTTA ATGGATTCCT CCTGGTTCTT 
CATAGTCAGA CAGACAAAAT ACCTTCTTGT CCTGATGGAT TGCCCAAGTT GTGGACTGGG 
TACAGTCTGC TTTATATTGA AGGCCAGGAG AAAGCACATA ATCAAGATCT GGGTCTCGCG 
GGATCCTGTC TTCCCCTGTT CAGCACGATG CCATTTGCTT ACTGCAACAT CAATCACGTC 
TGTTTCTATG CCAGGAGAAA TGATAAATCC TACTGGCTAT CCAGTGCCGC TCCTCTACCC 
ATGATGCCCC TCTCTGAAGA TAAAATACAG CCGTACATTA GCCGGTGTGT GGTATGCGAG 
TCCCCGGCCC AGGCCTTGGC CGTTCACAGC CAAGATGAAT CCATCCCTCC ATGTCCGACA 
TCATGGAGAA GCCTCTGGAT AGGTTATTCT TTCCTAATGC ACACAGGAGC TGGAGATCAA 
GGAGGAGGAC AGTCCCTTGT GTCCCCTGGA AGCTGCCTTG AAGACTTCCG AGCAGCCCCC 
TTCATTGAAT GCCAAGGACG GCAGGGAACG TGTCATTTTT TTGCCAATGA ATACAGCTTC 
TGGCTAACGA CAGTGAAACC AGAATTACAG TTTTCATCAG CTCCCTCACC AAGCACCTTA 
AAAGATGGAC AGGCACAGCG CCAAAAAATC AGCAGATGTC AGGTCTGCAT GAAATACAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028924801.1
CDS	323..5365
Translation	MALLRCYFRGIKNISKPWWLIIIFSLPVYGGGKKYTAICGGRNCSACQCFPEKGSRGQPGELGPQGPIGPCGASGPPGLPGEKGMRGDDGSAGAAGERGDKGPTGVPGFPGLDGVPGYPGPPGSRGKPGLDGHNGSRGDPGFPGQHGYPGQVGPAGLPGQKGEKGISAYVAEKGPPGDQGIPGPPGSPGPKGPRGAKGGMGFPGKPGADGPQGLPGLPGAQGNPGVGIKGQTGDPGEVGHQGPPGPPLLVGPPSSCLHKGEKGTKGMPGKSGLPGPVGAKGEPGSGVKGEKGIPGFPGLRGHSGSYGSPGSPGLKGEPGEVGIPGKFGFPGIKGDPGDRGEPGPPGIMVTPPVPLKGLPGDPGVPGPYGEMGSVGKVGPPGPSGKPGEDRAVGVTGPMGSRGAPGVPGPPGEVGVPGKADAPPGKPGNPGYPGTPGARGEQGLPGPDVVHCTSGPPGPQGMKGKAGPPGRSGVKGEKGNEGACACTPGVIGLPGTPGLPGRKGRKGELGYPGRFGEKGDQGSPGAKGYPGPSGIPGSLGMTGPKGEKGDRVIPRIKGRKGERGSQGPPGFPGLQGQPGQDGTEGPVGLPGLPGDRGGAVPGAKGFPGHQGAPGRTGPRGPSGLGFPGRQGMTGIPGDRGTVGDRGPPGAKGQKGDTFPCASDFPGNPGPPGFQGPPGPKGIQGPPGPAGPHGFDGAKGQRGKPGISEVPGPAGFPGDNGDPGFKGDKGTSPVGPQGFPGSPGINGQKGMPGDPAFGHPGLPGNKGSAGFPGPKGFRGDLGYPGSPGQAGTPGLPGTKGFKGQKGHNGPPGSPGPPGYLCEKGSPGLPGQPGLPGPQGNPGYPGLKGQQGDMGAPGPAGMKGLPGAPGLNGSSGPPGPPGNRGHLGDDGLPGFRGPKGAQGLPGIPGFPGELGKKGQAGHPGRKGERGGDGHPGHPGDKGDWGPKGEKGLPGDEGSISMISTKGEKGELGQPGEYGVPGERGDKGSSGLQGEEGLPGRDGLPGFQKGHPGPSGQPGFPGLPGFPGSPGSRGVFGFPGLPGDPGDDGSRGIPGPSGPIGVKGPKGKRGDSISLLGPLGEKGRPGNPGCAGHFGAPGDLGLPGPPGYPGTPGRPGPPGYPGQPGFPGTQGTPGQRGIPGEIGDPGISGPIGDQGLAGPRGMKGFSGIPGLNGLHGLKGPKGAKGSPGFIERGPPGFAGLPGAKGEKGEPGRVGLSFPGPFGERGPQGPPGRPGLQGSAGPKGRTPEGEISYPGPQGDIGFHGPDGIRGPPGSPGQPGGVDFWKGSPGDDGFPGPPGPSGPPGHPGPKGIPGCEGRDGQKGPMGHPGLQGPPGRPGPYGDKGSPGPPGTPGSPGPRGDPGLPGDIADCPKIPGPPGIPGSRGPEGRMGFPGPRGPPGLPGLKGEPGPDGQRGQDGFPGSPGAPGQKGDSGEPGCDGPPGPPGPTGDPGSRGPRTEYLNGFLLVLHSQTDKIPSCPDGLPKLWTGYSLLYIEGQEKAHNQDLGLAGSCLPLFSTMPFAYCNINHVCFYARRNDKSYWLSSAAPLPMMPLSEDKIQPYISRCVVCESPAQALAVHSQDESIPPCPTSWRSLWIGYSFLMHTGAGDQGGGQSLVSPGSCLEDFRAAPFIECQGRQGTCHFFANEYSFWLTTVKPELQFSSAPSPSTLKDGQAQRQKISRCQVCMKYS

Target ORF information:

RefSeq Version	XM_029068968.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029068968.1

ORF Insert Sequence:

ATGGCGCTAC TAAGATGTTA TTTCAGAGGG ATTAAAAATA TCTCCAAACC CTGGTGGCTC 
ATAATTATTT TTTCTCTACC GGTTTATGGG GGTGGAAAAA AATACACCGC CATTTGTGGA 
GGACGAAATT GTTCAGCTTG CCAATGTTTT CCTGAGAAAG GATCAAGGGG TCAGCCAGGA 
GAGCTTGGAC CACAGGGACC TATCGGACCA TGTGGAGCTT CAGGACCCCC TGGGTTGCCA 
GGAGAGAAAG GAATGAGAGG GGATGATGGA TCTGCGGGAG CAGCTGGAGA AAGGGGTGAT 
AAGGGCCCAA CAGGAGTTCC TGGCTTTCCA GGTTTGGATG GTGTGCCTGG CTACCCAGGA 
CCTCCCGGAT CAAGAGGTAA ACCGGGTTTA GATGGCCATA ATGGTTCAAG AGGAGATCCA 
GGCTTTCCTG GACAACATGG ATACCCAGGA CAAGTTGGCC CAGCTGGTCT ACCTGGACAA 
AAAGGAGAGA AAGGAATCTC AGCATATGTT GCAGAGAAAG GTCCACCTGG AGATCAAGGT 
ATCCCAGGAC CTCCTGGCTC ACCTGGACCA AAGGGCCCAA GGGGAGCAAA GGGCGGTATG 
GGGTTTCCGG GTAAACCAGG TGCAGATGGT CCTCAAGGCC TTCCTGGATT GCCAGGTGCG 
CAGGGCAATC CAGGAGTTGG CATTAAAGGA CAAACTGGAG ATCCAGGAGA AGTTGGACAT 
CAAGGGCCTC CAGGGCCACC CCTACTGGTT GGGCCTCCAA GTTCATGTCT CCACAAAGGA 
GAAAAAGGCA CAAAAGGGAT GCCTGGAAAA AGTGGCCTCC CTGGCCCAGT TGGAGCTAAG 
GGTGAACCTG GAAGTGGGGT GAAGGGAGAG AAAGGTATTC CTGGATTTCC TGGTCTTCGG 
GGTCATTCTG GTTCCTATGG ATCCCCAGGC TCTCCAGGAT TAAAGGGGGA ACCTGGAGAA 
GTTGGCATTC CTGGGAAGTT TGGATTTCCA GGCATAAAGG GAGATCCTGG AGACCGGGGG 
GAACCAGGGC CACCAGGTAT TATGGTAACT CCACCTGTTC CACTAAAAGG ACTTCCAGGA 
GATCCAGGAG TTCCAGGTCC CTATGGAGAA ATGGGATCAG TTGGAAAGGT TGGCCCACCT 
GGTCCTTCAG GTAAACCAGG TGAAGACCGG GCAGTAGGAG TGACAGGGCC AATGGGATCC 
CGAGGAGCAC CGGGTGTTCC AGGTCCCCCA GGGGAAGTTG GTGTTCCTGG AAAAGCAGAT 
GCCCCACCAG GAAAGCCTGG GAATCCAGGA TACCCAGGCA CACCAGGAGC ACGAGGAGAG 
CAGGGGCTGC CTGGACCAGA CGTTGTGCAC TGTACTTCTG GACCTCCTGG ACCTCAAGGA 
ATGAAAGGAA AAGCAGGACC TCCAGGAAGA AGTGGTGTGA AAGGTGAAAA AGGAAATGAA 
GGTGCCTGTG CCTGTACCCC TGGTGTCATT GGCCTACCAG GGACTCCAGG GCTCCCTGGA 
AGAAAAGGAA GGAAAGGGGA GCTGGGATAT CCTGGGAGAT TTGGAGAAAA AGGTGACCAA 
GGTTCTCCCG GGGCAAAAGG ATATCCAGGA CCTTCTGGTA TACCTGGTTC CTTGGGAATG 
ACTGGACCAA AAGGAGAAAA GGGAGACAGA GTTATCCCCA GAATAAAAGG AAGAAAAGGA 
GAAAGAGGCT CCCAAGGACC GCCTGGATTT CCAGGACTTC AAGGACAACC AGGGCAGGAT 
GGAACTGAAG GACCAGTAGG ATTACCTGGA TTGCCAGGTG ATCGAGGAGG CGCAGTTCCA 
GGTGCTAAGG GATTTCCCGG ACACCAAGGA GCCCCTGGGA GGACTGGACC TAGGGGTCCT 
TCGGGTTTGG GGTTTCCTGG ACGACAGGGC ATGACAGGCA TCCCAGGAGA CCGAGGTACC 
GTTGGTGACA GGGGACCACC TGGTGCAAAG GGCCAGAAAG GTGACACATT TCCCTGTGCC 
TCAGATTTTC CTGGAAATCC AGGCCCACCA GGCTTTCAAG GTCCTCCAGG TCCAAAGGGA 
ATTCAAGGAC CCCCAGGTCC AGCTGGACCC CATGGTTTTG ACGGGGCCAA AGGCCAGAGA 
GGCAAGCCAG GAATTTCCGA AGTGCCAGGA CCAGCTGGCT TTCCTGGTGA CAATGGAGAT 
CCAGGTTTTA AAGGTGACAA AGGAACCTCA CCAGTTGGTC CTCAGGGTTT TCCTGGTTCA 
CCTGGTATCA ATGGGCAAAA AGGTATGCCA GGTGATCCTG CTTTTGGCCA CCCAGGACTT 
CCAGGAAACA AAGGTTCTGC AGGATTTCCC GGACCTAAAG GATTTAGAGG TGACCTAGGA 
TATCCAGGAT CTCCAGGACA GGCTGGTACC CCAGGGCTTC CAGGAACCAA AGGATTTAAA 
GGCCAAAAAG GGCATAATGG ACCTCCGGGT TCTCCAGGTC CACCTGGATA CTTATGTGAA 
AAAGGCTCAC CTGGGTTGCC AGGACAACCA GGGCTCCCTG GACCACAAGG TAATCCAGGT 
TACCCTGGCT TGAAAGGCCA GCAAGGAGAT ATGGGTGCCC CTGGACCAGC TGGAATGAAG 
GGTCTTCCAG GAGCACCAGG ATTAAATGGA TCATCTGGTC CCCCAGGACC ACCAGGAAAT 
CGAGGGCATT TGGGGGATGA TGGGTTACCT GGATTTCGGG GCCCAAAGGG TGCCCAAGGG 
CTGCCGGGTA TCCCAGGATT CCCAGGCGAG CTAGGAAAGA AAGGGCAAGC AGGACACCCT 
GGGAGAAAGG GTGAACGGGG AGGAGATGGT CATCCTGGCC ACCCAGGAGA CAAAGGGGAC 
TGGGGACCTA AAGGTGAAAA AGGACTTCCT GGGGATGAAG GTAGTATTTC CATGATTTCC 
ACAAAGGGAG AAAAGGGGGA ATTAGGTCAA CCTGGCGAAT ATGGAGTCCC AGGAGAAAGA 
GGAGATAAAG GTTCTTCTGG ACTCCAAGGG GAAGAAGGAT TACCAGGAAG AGATGGACTT 
CCAGGGTTTC AGAAAGGTCA TCCTGGTCCA AGTGGCCAGC CTGGATTTCC AGGACTGCCT 
GGCTTCCCAG GCTCTCCAGG CTCAAGAGGA GTATTTGGTT TCCCTGGACT ACCGGGTGAT 
CCGGGAGATG ATGGTTCACG GGGCATACCT GGACCTTCAG GACCTATTGG AGTCAAAGGC 
CCCAAAGGAA AAAGAGGGGA TTCAATCAGT TTACTTGGCC CACTTGGAGA AAAAGGAAGA 
CCTGGAAACC CTGGATGTGC AGGTCATTTT GGAGCCCCAG GTGACCTGGG TTTGCCTGGG 
CCTCCGGGGT ATCCTGGAAC CCCAGGAAGG CCAGGACCAC CAGGATACCC AGGACAACCT 
GGCTTTCCAG GTACTCAGGG GACCCCTGGA CAAAGAGGAA TTCCAGGAGA AATAGGCGAC 
CCTGGGATCT CAGGACCTAT TGGAGATCAA GGACTTGCTG GGCCCCGAGG CATGAAAGGG 
TTCTCAGGAA TTCCAGGATT AAATGGTTTG CATGGACTCA AGGGGCCAAA AGGAGCGAAG 
GGTTCACCAG GTTTCATCGA AAGAGGACCA CCAGGTTTTG CTGGATTGCC TGGAGCAAAA 
GGGGAAAAAG GAGAACCTGG AAGGGTGGGA CTTTCTTTTC CAGGTCCTTT TGGTGAAAGA 
GGACCTCAAG GTCCCCCAGG GAGGCCTGGA CTTCAGGGAT CTGCAGGTCC TAAAGGAAGA 
ACACCTGAAG GAGAAATTTC TTATCCTGGT CCTCAAGGAG ATATAGGATT TCATGGCCCC 
GATGGTATAC GAGGTCCACC AGGGTCTCCA GGTCAACCTG GAGGTGTTGA CTTTTGGAAA 
GGGAGTCCAG GTGATGATGG CTTTCCAGGA CCACCAGGTC CCTCTGGTCC ACCAGGACAT 
CCTGGACCAA AAGGCATCCC AGGATGCGAA GGAAGAGATG GCCAAAAAGG GCCAATGGGA 
CATCCTGGTC TTCAAGGTCC ACCTGGACGC CCTGGGCCAT ATGGAGATAA AGGGAGTCCA 
GGACCCCCCG GGACTCCTGG GTCACCTGGT CCCAGAGGGG ACCCTGGCCT ACCTGGAGAT 
ATTGCAGATT GTCCCAAAAT CCCCGGGCCC CCAGGGATTC CAGGTTCAAG AGGACCAGAA 
GGACGCATGG GATTTCCCGG TCCAAGAGGA CCCCCAGGCT TACCAGGACT TAAAGGTGAA 
CCAGGGCCAG ATGGCCAGAG AGGTCAAGAT GGCTTTCCTG GATCGCCAGG TGCACCTGGA 
CAAAAGGGAG ACTCAGGGGA ACCAGGCTGT GATGGGCCAC CAGGTCCACC TGGCCCAACT 
GGAGATCCAG GATCCAGAGG GCCAAGAACT GAATACCTTA ATGGATTCCT CCTGGTTCTT 
CATAGTCAGA CAGACAAAAT ACCTTCTTGT CCTGATGGAT TGCCCAAGTT GTGGACTGGG 
TACAGTCTGC TTTATATTGA AGGCCAGGAG AAAGCACATA ATCAAGATCT GGGTCTCGCG 
GGATCCTGTC TTCCCCTGTT CAGCACGATG CCATTTGCTT ACTGCAACAT CAATCACGTC 
TGTTTCTATG CCAGGAGAAA TGATAAATCC TACTGGCTAT CCAGTGCCGC TCCTCTACCC 
ATGATGCCCC TCTCTGAAGA TAAAATACAG CCGTACATTA GCCGGTGTGT GGTATGCGAG 
TCCCCGGCCC AGGCCTTGGC CGTTCACAGC CAAGATGAAT CCATCCCTCC ATGTCCGACA 
TCATGGAGAA GCCTCTGGAT AGGTTATTCT TTCCTAATGC ACACAGGAGC TGGAGATCAA 
GGAGGAGGAC AGTCCCTTGT GTCCCCTGGA AGCTGCCTTG AAGACTTCCG AGCAGCCCCC 
TTCATTGAAT GCCAAGGACG GCAGGGAACG TGTCATTTTT TTGCCAATGA ATACAGCTTC 
TGGCTAACGA CAGTGAAACC AGAATTACAG TTTTCATCAG CTCCCTCACC AAGCACCTTA 
AAAGATGGAC AGGCACAGCG CCAAAAAATC AGCAGATGTC AGGTCTGCAT GAAATACAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_029068969.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028924802.1 Ornithorhynchus anatinus collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

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CloneID	OOc132061
Clone ID Related Accession (Same CDS sequence)	XM_029068969.1
Accession Version	XM_029068969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	collagen alpha-4(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAC TAAGATGTTA TTTCAGAGGG ATTAAAAATA TCTCCAAACC CTGGTGGCTC 
ATAATTATTT TTTCTCTACC GGTTTATGGG GGTGGAAAAA AATACACCGC CATTTGTGGA 
GGACGAAATT GTTCAGCTTG CCAATGTTTT CCTGAGAAAG GATCAAGGGG TCAGCCAGGA 
GAGCTTGGAC CACAGGGACC TATCGGACCA TGTGGAGCTT CAGGACCCCC TGGGTTGCCA 
GGAGAGAAAG GAATGAGAGG GGATGATGGA TCTGCGGGAG CAGCTGGAGA AAGGGGTGAT 
AAGGGCCCAA CAGGAGTTCC TGGCTTTCCA GGTTTGGATG GTGTGCCTGG CTACCCAGGA 
CCTCCCGGAT CAAGAGGTAA ACCGGGTTTA GATGGCCATA ATGGTTCAAG AGGAGATCCA 
GGCTTTCCTG GACAACATGG ATACCCAGGA CAAGTTGGCC CAGCTGGTCT ACCTGGACAA 
AAAGGAGAGA AAGGAATCTC AGCATATGTT GCAGAGAAAG GTCCACCTGG AGATCAAGGT 
ATCCCAGGAC CTCCTGGCTC ACCTGGACCA AAGGGCCCAA GGGGAGCAAA GGGCGGTATG 
GGGTTTCCGG GTAAACCAGG TGCAGATGGT CCTCAAGGCC TTCCTGGATT GCCAGGTGCG 
CAGGGCAATC CAGGAGTTGG CATTAAAGGA CAAACTGGAG ATCCAGGAGA AGTTGGACAT 
CAAGGGCCTC CAGGGCCACC CCTACTGGTT GGGCCTCCAA GTTCATGTCT CCACAAAGGA 
GAAAAAGGCA CAAAAGGGAT GCCTGGAAAA AGTGGCCTCC CTGGCCCAGT TGGAGCTAAG 
GGTGAACCTG GAAGTGGGGT GAAGGGAGAG AAAGGTATTC CTGGATTTCC TGGTCTTCGG 
GGTCATTCTG GTTCCTATGG ATCCCCAGGC TCTCCAGGAT TAAAGGGGGA ACCTGGAGAA 
GTTGGCATTC CTGGGAAGTT TGGATTTCCA GGCATAAAGG GAGATCCTGG AGACCGGGGG 
GAACCAGGGC CACCAGGTAT TATGGTAACT CCACCTGTTC CACTAAAAGG ACTTCCAGGA 
GATCCAGGAG TTCCAGGTCC CTATGGAGAA ATGGGATCAG TTGGAAAGGT TGGCCCACCT 
GGTCCTTCAG GTAAACCAGG TGAAGACCGG GCAGGAGTGA CAGGGCCAAT GGGATCCCGA 
GGAGCACCGG GTGTTCCAGG TCCCCCAGGG GAAGTTGGTG TTCCTGGAAA AGCAGATGCC 
CCACCAGGAA AGCCTGGGAA TCCAGGATAC CCAGGCACAC CAGGAGCACG AGGAGAGCAG 
GGGCTGCCTG GACCAGACGT TGTGCACTGT ACTTCTGGAC CTCCTGGACC TCAAGGAATG 
AAAGGAAAAG CAGGACCTCC AGGAAGAAGT GGTGTGAAAG GTGAAAAAGG AAATGAAGGT 
GCCTGTGCCT GTACCCCTGG TGTCATTGGC CTACCAGGGA CTCCAGGGCT CCCTGGAAGA 
AAAGGAAGGA AAGGGGAGCT GGGATATCCT GGGAGATTTG GAGAAAAAGG TGACCAAGGT 
TCTCCCGGGG CAAAAGGATA TCCAGGACCT TCTGGTATAC CTGGTTCCTT GGGAATGACT 
GGACCAAAAG GAGAAAAGGG AGACAGAGTT ATCCCCAGAA TAAAAGGAAG AAAAGGAGAA 
AGAGGCTCCC AAGGACCGCC TGGATTTCCA GGACTTCAAG GACAACCAGG GCAGGATGGA 
ACTGAAGGAC CAGTAGGATT ACCTGGATTG CCAGGTGATC GAGGAGGCGC AGTTCCAGGT 
GCTAAGGGAT TTCCCGGACA CCAAGGAGCC CCTGGGAGGA CTGGACCTAG GGGTCCTTCG 
GGTTTGGGGT TTCCTGGACG ACAGGGCATG ACAGGCATCC CAGGAGACCG AGGTACCGTT 
GGTGACAGGG GACCACCTGG TGCAAAGGGC CAGAAAGGTG ACACATTTCC CTGTGCCTCA 
GATTTTCCTG GAAATCCAGG CCCACCAGGC TTTCAAGGTC CTCCAGGTCC AAAGGGAATT 
CAAGGACCCC CAGGTCCAGC TGGACCCCAT GGTTTTGACG GGGCCAAAGG CCAGAGAGGC 
AAGCCAGGAA TTTCCGAAGT GCCAGGACCA GCTGGCTTTC CTGGTGACAA TGGAGATCCA 
GGTTTTAAAG GTGACAAAGG AACCTCACCA GTTGGTCCTC AGGGTTTTCC TGGTTCACCT 
GGTATCAATG GGCAAAAAGG TATGCCAGGT GATCCTGCTT TTGGCCACCC AGGACTTCCA 
GGAAACAAAG GTTCTGCAGG ATTTCCCGGA CCTAAAGGAT TTAGAGGTGA CCTAGGATAT 
CCAGGATCTC CAGGACAGGC TGGTACCCCA GGGCTTCCAG GAACCAAAGG ATTTAAAGGC 
CAAAAAGGGC ATAATGGACC TCCGGGTTCT CCAGGTCCAC CTGGATACTT ATGTGAAAAA 
GGCTCACCTG GGTTGCCAGG ACAACCAGGG CTCCCTGGAC CACAAGGTAA TCCAGGTTAC 
CCTGGCTTGA AAGGCCAGCA AGGAGATATG GGTGCCCCTG GACCAGCTGG AATGAAGGGT 
CTTCCAGGAG CACCAGGATT AAATGGATCA TCTGGTCCCC CAGGACCACC AGGAAATCGA 
GGGCATTTGG GGGATGATGG GTTACCTGGA TTTCGGGGCC CAAAGGGTGC CCAAGGGCTG 
CCGGGTATCC CAGGATTCCC AGGCGAGCTA GGAAAGAAAG GGCAAGCAGG ACACCCTGGG 
AGAAAGGGTG AACGGGGAGG AGATGGTCAT CCTGGCCACC CAGGAGACAA AGGGGACTGG 
GGACCTAAAG GTGAAAAAGG ACTTCCTGGG GATGAAGGTA GTATTTCCAT GATTTCCACA 
AAGGGAGAAA AGGGGGAATT AGGTCAACCT GGCGAATATG GAGTCCCAGG AGAAAGAGGA 
GATAAAGGTT CTTCTGGACT CCAAGGGGAA GAAGGATTAC CAGGAAGAGA TGGACTTCCA 
GGGTTTCAGA AAGGTCATCC TGGTCCAAGT GGCCAGCCTG GATTTCCAGG ACTGCCTGGC 
TTCCCAGGCT CTCCAGGCTC AAGAGGAGTA TTTGGTTTCC CTGGACTACC GGGTGATCCG 
GGAGATGATG GTTCACGGGG CATACCTGGA CCTTCAGGAC CTATTGGAGT CAAAGGCCCC 
AAAGGAAAAA GAGGGGATTC AATCAGTTTA CTTGGCCCAC TTGGAGAAAA AGGAAGACCT 
GGAAACCCTG GATGTGCAGG TCATTTTGGA GCCCCAGGTG ACCTGGGTTT GCCTGGGCCT 
CCGGGGTATC CTGGAACCCC AGGAAGGCCA GGACCACCAG GATACCCAGG ACAACCTGGC 
TTTCCAGGTA CTCAGGGGAC CCCTGGACAA AGAGGAATTC CAGGAGAAAT AGGCGACCCT 
GGGATCTCAG GACCTATTGG AGATCAAGGA CTTGCTGGGC CCCGAGGCAT GAAAGGGTTC 
TCAGGAATTC CAGGATTAAA TGGTTTGCAT GGACTCAAGG GGCCAAAAGG AGCGAAGGGT 
TCACCAGGTT TCATCGAAAG AGGACCACCA GGTTTTGCTG GATTGCCTGG AGCAAAAGGG 
GAAAAAGGAG AACCTGGAAG GGTGGGACTT TCTTTTCCAG GTCCTTTTGG TGAAAGAGGA 
CCTCAAGGTC CCCCAGGGAG GCCTGGACTT CAGGGATCTG CAGGTCCTAA AGGAAGAACA 
CCTGAAGGAG AAATTTCTTA TCCTGGTCCT CAAGGAGATA TAGGATTTCA TGGCCCCGAT 
GGTATACGAG GTCCACCAGG GTCTCCAGGT CAACCTGGAG GTGTTGACTT TTGGAAAGGG 
AGTCCAGGTG ATGATGGCTT TCCAGGACCA CCAGGTCCCT CTGGTCCACC AGGACATCCT 
GGACCAAAAG GCATCCCAGG ATGCGAAGGA AGAGATGGCC AAAAAGGGCC AATGGGACAT 
CCTGGTCTTC AAGGTCCACC TGGACGCCCT GGGCCATATG GAGATAAAGG GAGTCCAGGA 
CCCCCCGGGA CTCCTGGGTC ACCTGGTCCC AGAGGGGACC CTGGCCTACC TGGAGATATT 
GCAGATTGTC CCAAAATCCC CGGGCCCCCA GGGATTCCAG GTTCAAGAGG ACCAGAAGGA 
CGCATGGGAT TTCCCGGTCC AAGAGGACCC CCAGGCTTAC CAGGACTTAA AGGTGAACCA 
GGGCCAGATG GCCAGAGAGG TCAAGATGGC TTTCCTGGAT CGCCAGGTGC ACCTGGACAA 
AAGGGAGACT CAGGGGAACC AGGCTGTGAT GGGCCACCAG GTCCACCTGG CCCAACTGGA 
GATCCAGGAT CCAGAGGGCC AAGAACTGAA TACCTTAATG GATTCCTCCT GGTTCTTCAT 
AGTCAGACAG ACAAAATACC TTCTTGTCCT GATGGATTGC CCAAGTTGTG GACTGGGTAC 
AGTCTGCTTT ATATTGAAGG CCAGGAGAAA GCACATAATC AAGATCTGGG TCTCGCGGGA 
TCCTGTCTTC CCCTGTTCAG CACGATGCCA TTTGCTTACT GCAACATCAA TCACGTCTGT 
TTCTATGCCA GGAGAAATGA TAAATCCTAC TGGCTATCCA GTGCCGCTCC TCTACCCATG 
ATGCCCCTCT CTGAAGATAA AATACAGCCG TACATTAGCC GGTGTGTGGT ATGCGAGTCC 
CCGGCCCAGG CCTTGGCCGT TCACAGCCAA GATGAATCCA TCCCTCCATG TCCGACATCA 
TGGAGAAGCC TCTGGATAGG TTATTCTTTC CTAATGCACA CAGGAGCTGG AGATCAAGGA 
GGAGGACAGT CCCTTGTGTC CCCTGGAAGC TGCCTTGAAG ACTTCCGAGC AGCCCCCTTC 
ATTGAATGCC AAGGACGGCA GGGAACGTGT CATTTTTTTG CCAATGAATA CAGCTTCTGG 
CTAACGACAG TGAAACCAGA ATTACAGTTT TCATCAGCTC CCTCACCAAG CACCTTAAAA 
GATGGACAGG CACAGCGCCA AAAAATCAGC AGATGTCAGG TCTGCATGAA ATACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028924802.1
CDS	222..5261
Translation	MALLRCYFRGIKNISKPWWLIIIFSLPVYGGGKKYTAICGGRNCSACQCFPEKGSRGQPGELGPQGPIGPCGASGPPGLPGEKGMRGDDGSAGAAGERGDKGPTGVPGFPGLDGVPGYPGPPGSRGKPGLDGHNGSRGDPGFPGQHGYPGQVGPAGLPGQKGEKGISAYVAEKGPPGDQGIPGPPGSPGPKGPRGAKGGMGFPGKPGADGPQGLPGLPGAQGNPGVGIKGQTGDPGEVGHQGPPGPPLLVGPPSSCLHKGEKGTKGMPGKSGLPGPVGAKGEPGSGVKGEKGIPGFPGLRGHSGSYGSPGSPGLKGEPGEVGIPGKFGFPGIKGDPGDRGEPGPPGIMVTPPVPLKGLPGDPGVPGPYGEMGSVGKVGPPGPSGKPGEDRAGVTGPMGSRGAPGVPGPPGEVGVPGKADAPPGKPGNPGYPGTPGARGEQGLPGPDVVHCTSGPPGPQGMKGKAGPPGRSGVKGEKGNEGACACTPGVIGLPGTPGLPGRKGRKGELGYPGRFGEKGDQGSPGAKGYPGPSGIPGSLGMTGPKGEKGDRVIPRIKGRKGERGSQGPPGFPGLQGQPGQDGTEGPVGLPGLPGDRGGAVPGAKGFPGHQGAPGRTGPRGPSGLGFPGRQGMTGIPGDRGTVGDRGPPGAKGQKGDTFPCASDFPGNPGPPGFQGPPGPKGIQGPPGPAGPHGFDGAKGQRGKPGISEVPGPAGFPGDNGDPGFKGDKGTSPVGPQGFPGSPGINGQKGMPGDPAFGHPGLPGNKGSAGFPGPKGFRGDLGYPGSPGQAGTPGLPGTKGFKGQKGHNGPPGSPGPPGYLCEKGSPGLPGQPGLPGPQGNPGYPGLKGQQGDMGAPGPAGMKGLPGAPGLNGSSGPPGPPGNRGHLGDDGLPGFRGPKGAQGLPGIPGFPGELGKKGQAGHPGRKGERGGDGHPGHPGDKGDWGPKGEKGLPGDEGSISMISTKGEKGELGQPGEYGVPGERGDKGSSGLQGEEGLPGRDGLPGFQKGHPGPSGQPGFPGLPGFPGSPGSRGVFGFPGLPGDPGDDGSRGIPGPSGPIGVKGPKGKRGDSISLLGPLGEKGRPGNPGCAGHFGAPGDLGLPGPPGYPGTPGRPGPPGYPGQPGFPGTQGTPGQRGIPGEIGDPGISGPIGDQGLAGPRGMKGFSGIPGLNGLHGLKGPKGAKGSPGFIERGPPGFAGLPGAKGEKGEPGRVGLSFPGPFGERGPQGPPGRPGLQGSAGPKGRTPEGEISYPGPQGDIGFHGPDGIRGPPGSPGQPGGVDFWKGSPGDDGFPGPPGPSGPPGHPGPKGIPGCEGRDGQKGPMGHPGLQGPPGRPGPYGDKGSPGPPGTPGSPGPRGDPGLPGDIADCPKIPGPPGIPGSRGPEGRMGFPGPRGPPGLPGLKGEPGPDGQRGQDGFPGSPGAPGQKGDSGEPGCDGPPGPPGPTGDPGSRGPRTEYLNGFLLVLHSQTDKIPSCPDGLPKLWTGYSLLYIEGQEKAHNQDLGLAGSCLPLFSTMPFAYCNINHVCFYARRNDKSYWLSSAAPLPMMPLSEDKIQPYISRCVVCESPAQALAVHSQDESIPPCPTSWRSLWIGYSFLMHTGAGDQGGGQSLVSPGSCLEDFRAAPFIECQGRQGTCHFFANEYSFWLTTVKPELQFSSAPSPSTLKDGQAQRQKISRCQVCMKYS

Target ORF information:

RefSeq Version	XM_029068969.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029068969.1

ORF Insert Sequence:

ATGGCGCTAC TAAGATGTTA TTTCAGAGGG ATTAAAAATA TCTCCAAACC CTGGTGGCTC 
ATAATTATTT TTTCTCTACC GGTTTATGGG GGTGGAAAAA AATACACCGC CATTTGTGGA 
GGACGAAATT GTTCAGCTTG CCAATGTTTT CCTGAGAAAG GATCAAGGGG TCAGCCAGGA 
GAGCTTGGAC CACAGGGACC TATCGGACCA TGTGGAGCTT CAGGACCCCC TGGGTTGCCA 
GGAGAGAAAG GAATGAGAGG GGATGATGGA TCTGCGGGAG CAGCTGGAGA AAGGGGTGAT 
AAGGGCCCAA CAGGAGTTCC TGGCTTTCCA GGTTTGGATG GTGTGCCTGG CTACCCAGGA 
CCTCCCGGAT CAAGAGGTAA ACCGGGTTTA GATGGCCATA ATGGTTCAAG AGGAGATCCA 
GGCTTTCCTG GACAACATGG ATACCCAGGA CAAGTTGGCC CAGCTGGTCT ACCTGGACAA 
AAAGGAGAGA AAGGAATCTC AGCATATGTT GCAGAGAAAG GTCCACCTGG AGATCAAGGT 
ATCCCAGGAC CTCCTGGCTC ACCTGGACCA AAGGGCCCAA GGGGAGCAAA GGGCGGTATG 
GGGTTTCCGG GTAAACCAGG TGCAGATGGT CCTCAAGGCC TTCCTGGATT GCCAGGTGCG 
CAGGGCAATC CAGGAGTTGG CATTAAAGGA CAAACTGGAG ATCCAGGAGA AGTTGGACAT 
CAAGGGCCTC CAGGGCCACC CCTACTGGTT GGGCCTCCAA GTTCATGTCT CCACAAAGGA 
GAAAAAGGCA CAAAAGGGAT GCCTGGAAAA AGTGGCCTCC CTGGCCCAGT TGGAGCTAAG 
GGTGAACCTG GAAGTGGGGT GAAGGGAGAG AAAGGTATTC CTGGATTTCC TGGTCTTCGG 
GGTCATTCTG GTTCCTATGG ATCCCCAGGC TCTCCAGGAT TAAAGGGGGA ACCTGGAGAA 
GTTGGCATTC CTGGGAAGTT TGGATTTCCA GGCATAAAGG GAGATCCTGG AGACCGGGGG 
GAACCAGGGC CACCAGGTAT TATGGTAACT CCACCTGTTC CACTAAAAGG ACTTCCAGGA 
GATCCAGGAG TTCCAGGTCC CTATGGAGAA ATGGGATCAG TTGGAAAGGT TGGCCCACCT 
GGTCCTTCAG GTAAACCAGG TGAAGACCGG GCAGGAGTGA CAGGGCCAAT GGGATCCCGA 
GGAGCACCGG GTGTTCCAGG TCCCCCAGGG GAAGTTGGTG TTCCTGGAAA AGCAGATGCC 
CCACCAGGAA AGCCTGGGAA TCCAGGATAC CCAGGCACAC CAGGAGCACG AGGAGAGCAG 
GGGCTGCCTG GACCAGACGT TGTGCACTGT ACTTCTGGAC CTCCTGGACC TCAAGGAATG 
AAAGGAAAAG CAGGACCTCC AGGAAGAAGT GGTGTGAAAG GTGAAAAAGG AAATGAAGGT 
GCCTGTGCCT GTACCCCTGG TGTCATTGGC CTACCAGGGA CTCCAGGGCT CCCTGGAAGA 
AAAGGAAGGA AAGGGGAGCT GGGATATCCT GGGAGATTTG GAGAAAAAGG TGACCAAGGT 
TCTCCCGGGG CAAAAGGATA TCCAGGACCT TCTGGTATAC CTGGTTCCTT GGGAATGACT 
GGACCAAAAG GAGAAAAGGG AGACAGAGTT ATCCCCAGAA TAAAAGGAAG AAAAGGAGAA 
AGAGGCTCCC AAGGACCGCC TGGATTTCCA GGACTTCAAG GACAACCAGG GCAGGATGGA 
ACTGAAGGAC CAGTAGGATT ACCTGGATTG CCAGGTGATC GAGGAGGCGC AGTTCCAGGT 
GCTAAGGGAT TTCCCGGACA CCAAGGAGCC CCTGGGAGGA CTGGACCTAG GGGTCCTTCG 
GGTTTGGGGT TTCCTGGACG ACAGGGCATG ACAGGCATCC CAGGAGACCG AGGTACCGTT 
GGTGACAGGG GACCACCTGG TGCAAAGGGC CAGAAAGGTG ACACATTTCC CTGTGCCTCA 
GATTTTCCTG GAAATCCAGG CCCACCAGGC TTTCAAGGTC CTCCAGGTCC AAAGGGAATT 
CAAGGACCCC CAGGTCCAGC TGGACCCCAT GGTTTTGACG GGGCCAAAGG CCAGAGAGGC 
AAGCCAGGAA TTTCCGAAGT GCCAGGACCA GCTGGCTTTC CTGGTGACAA TGGAGATCCA 
GGTTTTAAAG GTGACAAAGG AACCTCACCA GTTGGTCCTC AGGGTTTTCC TGGTTCACCT 
GGTATCAATG GGCAAAAAGG TATGCCAGGT GATCCTGCTT TTGGCCACCC AGGACTTCCA 
GGAAACAAAG GTTCTGCAGG ATTTCCCGGA CCTAAAGGAT TTAGAGGTGA CCTAGGATAT 
CCAGGATCTC CAGGACAGGC TGGTACCCCA GGGCTTCCAG GAACCAAAGG ATTTAAAGGC 
CAAAAAGGGC ATAATGGACC TCCGGGTTCT CCAGGTCCAC CTGGATACTT ATGTGAAAAA 
GGCTCACCTG GGTTGCCAGG ACAACCAGGG CTCCCTGGAC CACAAGGTAA TCCAGGTTAC 
CCTGGCTTGA AAGGCCAGCA AGGAGATATG GGTGCCCCTG GACCAGCTGG AATGAAGGGT 
CTTCCAGGAG CACCAGGATT AAATGGATCA TCTGGTCCCC CAGGACCACC AGGAAATCGA 
GGGCATTTGG GGGATGATGG GTTACCTGGA TTTCGGGGCC CAAAGGGTGC CCAAGGGCTG 
CCGGGTATCC CAGGATTCCC AGGCGAGCTA GGAAAGAAAG GGCAAGCAGG ACACCCTGGG 
AGAAAGGGTG AACGGGGAGG AGATGGTCAT CCTGGCCACC CAGGAGACAA AGGGGACTGG 
GGACCTAAAG GTGAAAAAGG ACTTCCTGGG GATGAAGGTA GTATTTCCAT GATTTCCACA 
AAGGGAGAAA AGGGGGAATT AGGTCAACCT GGCGAATATG GAGTCCCAGG AGAAAGAGGA 
GATAAAGGTT CTTCTGGACT CCAAGGGGAA GAAGGATTAC CAGGAAGAGA TGGACTTCCA 
GGGTTTCAGA AAGGTCATCC TGGTCCAAGT GGCCAGCCTG GATTTCCAGG ACTGCCTGGC 
TTCCCAGGCT CTCCAGGCTC AAGAGGAGTA TTTGGTTTCC CTGGACTACC GGGTGATCCG 
GGAGATGATG GTTCACGGGG CATACCTGGA CCTTCAGGAC CTATTGGAGT CAAAGGCCCC 
AAAGGAAAAA GAGGGGATTC AATCAGTTTA CTTGGCCCAC TTGGAGAAAA AGGAAGACCT 
GGAAACCCTG GATGTGCAGG TCATTTTGGA GCCCCAGGTG ACCTGGGTTT GCCTGGGCCT 
CCGGGGTATC CTGGAACCCC AGGAAGGCCA GGACCACCAG GATACCCAGG ACAACCTGGC 
TTTCCAGGTA CTCAGGGGAC CCCTGGACAA AGAGGAATTC CAGGAGAAAT AGGCGACCCT 
GGGATCTCAG GACCTATTGG AGATCAAGGA CTTGCTGGGC CCCGAGGCAT GAAAGGGTTC 
TCAGGAATTC CAGGATTAAA TGGTTTGCAT GGACTCAAGG GGCCAAAAGG AGCGAAGGGT 
TCACCAGGTT TCATCGAAAG AGGACCACCA GGTTTTGCTG GATTGCCTGG AGCAAAAGGG 
GAAAAAGGAG AACCTGGAAG GGTGGGACTT TCTTTTCCAG GTCCTTTTGG TGAAAGAGGA 
CCTCAAGGTC CCCCAGGGAG GCCTGGACTT CAGGGATCTG CAGGTCCTAA AGGAAGAACA 
CCTGAAGGAG AAATTTCTTA TCCTGGTCCT CAAGGAGATA TAGGATTTCA TGGCCCCGAT 
GGTATACGAG GTCCACCAGG GTCTCCAGGT CAACCTGGAG GTGTTGACTT TTGGAAAGGG 
AGTCCAGGTG ATGATGGCTT TCCAGGACCA CCAGGTCCCT CTGGTCCACC AGGACATCCT 
GGACCAAAAG GCATCCCAGG ATGCGAAGGA AGAGATGGCC AAAAAGGGCC AATGGGACAT 
CCTGGTCTTC AAGGTCCACC TGGACGCCCT GGGCCATATG GAGATAAAGG GAGTCCAGGA 
CCCCCCGGGA CTCCTGGGTC ACCTGGTCCC AGAGGGGACC CTGGCCTACC TGGAGATATT 
GCAGATTGTC CCAAAATCCC CGGGCCCCCA GGGATTCCAG GTTCAAGAGG ACCAGAAGGA 
CGCATGGGAT TTCCCGGTCC AAGAGGACCC CCAGGCTTAC CAGGACTTAA AGGTGAACCA 
GGGCCAGATG GCCAGAGAGG TCAAGATGGC TTTCCTGGAT CGCCAGGTGC ACCTGGACAA 
AAGGGAGACT CAGGGGAACC AGGCTGTGAT GGGCCACCAG GTCCACCTGG CCCAACTGGA 
GATCCAGGAT CCAGAGGGCC AAGAACTGAA TACCTTAATG GATTCCTCCT GGTTCTTCAT 
AGTCAGACAG ACAAAATACC TTCTTGTCCT GATGGATTGC CCAAGTTGTG GACTGGGTAC 
AGTCTGCTTT ATATTGAAGG CCAGGAGAAA GCACATAATC AAGATCTGGG TCTCGCGGGA 
TCCTGTCTTC CCCTGTTCAG CACGATGCCA TTTGCTTACT GCAACATCAA TCACGTCTGT 
TTCTATGCCA GGAGAAATGA TAAATCCTAC TGGCTATCCA GTGCCGCTCC TCTACCCATG 
ATGCCCCTCT CTGAAGATAA AATACAGCCG TACATTAGCC GGTGTGTGGT ATGCGAGTCC 
CCGGCCCAGG CCTTGGCCGT TCACAGCCAA GATGAATCCA TCCCTCCATG TCCGACATCA 
TGGAGAAGCC TCTGGATAGG TTATTCTTTC CTAATGCACA CAGGAGCTGG AGATCAAGGA 
GGAGGACAGT CCCTTGTGTC CCCTGGAAGC TGCCTTGAAG ACTTCCGAGC AGCCCCCTTC 
ATTGAATGCC AAGGACGGCA GGGAACGTGT CATTTTTTTG CCAATGAATA CAGCTTCTGG 
CTAACGACAG TGAAACCAGA ATTACAGTTT TCATCAGCTC CCTCACCAAG CACCTTAAAA 
GATGGACAGG CACAGCGCCA AAAAATCAGC AGATGTCAGG TCTGCATGAA ATACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_029068970.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028924803.1 Ornithorhynchus anatinus collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.

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CloneID	OOc132062
Clone ID Related Accession (Same CDS sequence)	XM_029068970.1
Accession Version	XM_029068970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	collagen alpha-4(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAC TAAGATGTTA TTTCAGAGGG ATTAAAAATA TCTCCAAACC CTGGTGGCTC 
ATAATTATTT TTTCTCTACC GGTTTATGGG GGTGGAAAAA AATACACCGC CATTTGTGGA 
GGACGAAATT GTTCAGCTTG CCAATGTTTT CCTGAGAAAG GATCAAGGGG TCAGCCAGGA 
GAGCTTGGAC CACAGGGACC TATCGGACCA TGTGGAGCTT CAGGACCCCC TGGGTTGCCA 
GGAGAGAAAG GAATGAGAGG GGATGATGGA TCTGCGGGAG CAGCTGGAGA AAGGGGTGAT 
AAGGGCCCAA CAGGAGTTCC TGGCTTTCCA GGTTTGGATG GTGTGCCTGG CTACCCAGGA 
CCTCCCGGAT CAAGAGGTAA ACCGGGTTTA GATGGCCATA ATGGTTCAAG AGGAGATCCA 
GGCTTTCCTG GACAACATGG ATACCCAGGA CAAGTTGGCC CAGCTGGTCT ACCTGGACAA 
AAAGGAGAGA AAGGAATCTC AGCATATGTT GCAGAGAAAG GTCCACCTGG AGATCAAGGT 
ATCCCAGGAC CTCCTGGCTC ACCTGGACCA AAGGGCCCAA GGGGAGCAAA GGGCGGTATG 
GGGTTTCCGG GTAAACCAGG TGCAGATGGT CCTCAAGGCC TTCCTGGATT GCCAGGTGCG 
CAGGGCAATC CAGGAGTTGG CATTAAAGGA CAAACTGGAG ATCCAGGAGA AGTTGGACAT 
CAAGGGCCTC CAGGGCCACC CCTACTGGTT GGGCCTCCAA GTTCATGTCT CCACAAAGGA 
GAAAAAGGCA CAAAAGGGAT GCCTGGAAAA AGTGGCCTCC CTGGCCCAGT TGGAGCTAAG 
GGTGAACCTG GAAGTGGGGT GAAGGGAGAG AAAGGTATTC CTGGATTTCC TGGTCTTCGG 
GGTCATTCTG GTTCCTATGG ATCCCCAGGC TCTCCAGGAT TAAAGGGGGA ACCTGGAGAA 
GTTGGCATTC CTGGGAAGTT TGGATTTCCA GGCATAAAGG GAGATCCTGG AGACCGGGGG 
GAACCAGGGC CACCAGGTAT TATGGTAACT CCACCTGTTC CACTAAAAGG ACTTCCAGGA 
GATCCAGGAG TTCCAGGTCC CTATGGAGAA ATGGGATCAG TTGGAAAGGT TGGCCCACCT 
GGTCCTTCAG GTAAACCAGG TGAAGACCGG GCAGTAGGAG TGACAGGGCC AATGGGATCC 
CGAGGAGCAC CGGGTGTTCC AGGTCCCCCA GGGGAAGTTG GTGTTCCTGG AAAAGCAGAT 
GCCCCACCAG GAAAGCCTGG GAATCCAGGA TACCCAGGCA CACCAGGAGC ACGAGGAGAG 
CAGGGGCTGC CTGGACCAGA CGTTGTGCAC TGTACTTCTG GACCTCCTGG ACCTCAAGGA 
ATGAAAGGAA AAGCAGGACC TCCAGGAAGA AGTGGTGTGA AAGGTGAAAA AGGAAATGAA 
GGTGCCTGTG CCTGTACCCC TGGTGTCATT GGCCTACCAG GGACTCCAGG GCTCCCTGGA 
AGAAAAGGAA GGAAAGGGGA GCTGGGATAT CCTGGGAGAT TTGGAGAAAA AGGTGACCAA 
GGTTCTCCCG GGGCAAAAGG ATATCCAGGA CCTTCTGGTA TACCTGGTTC CTTGGGAATG 
ACTGGACCAA AAGGAGAAAA GGGAGACAGA GTTATCCCCA GAATAAAAGG AAGAAAAGGA 
GAAAGAGGCT CCCAAGGACC GCCTGGATTT CCAGGACTTC AAGGACAACC AGGGCAGGAT 
GGAACTGAAG GACCAGTAGG ATTACCTGGA TTGCCAGGTG ATCGAGGAGG CGCAGTTCCA 
GGTGCTAAGG GATTTCCCGG ACACCAAGGA GCCCCTGGGA GGACTGGACC TAGGGGTCCT 
TCGGGTTTGG GGTTTCCTGG ACGACAGGGC ATGACAGGCA TCCCAGGAGA CCGAGGTACC 
GTTGGTGACA GGGGACCACC TGGTGCAAAG GGCCAGAAAG GTGACACATT TCCCTGTGCC 
TCAGATTTTC CTGGAAATCC AGGCCCACCA GGCTTTCAAG GTCCTCCAGG TCCAAAGGGA 
ATTCAAGGAC CCCCAGGTCC AGCTGGACCC CATGGTTTTG ACGGGGCCAA AGGCCAGAGA 
GGCAAGCCAG GAATTTCCGA AGTGCCAGGA CCAGCTGGCT TTCCTGGTGA CAATGGAGAT 
CCAGGTTTTA AAGGTGACAA AGGAACCTCA CCAGTTGGTC CTCAGGGTTT TCCTGGTTCA 
CCTGGTATCA ATGGGCAAAA AGGTATGCCA GGTGATCCTG CTTTTGGCCA CCCAGGACTT 
CCAGGAAACA AAGGTTCTGC AGGATTTCCC GGACCTAAAG GATTTAGAGG TGACCTAGGA 
TATCCAGGAT CTCCAGGACA GGCTGGTACC CCAGGGCTTC CAGGAACCAA AGGATTTAAA 
GGCCAAAAAG GGCATAATGG ACCTCCGGGT TCTCCAGGTC CACCTGGATA CTTATGTGAA 
AAAGGCTCAC CTGGGTTGCC AGGACAACCA GGGCTCCCTG GACCACAAGG TAATCCAGGT 
TACCCTGGCT TGAAAGGCCA GCAAGGAGAT ATGGGTGCCC CTGGACCAGC TGGAATGAAG 
GGTCTTCCAG GAGCACCAGG ATTAAATGGA TCATCTGGTC CCCCAGGACC ACCAGGAAAT 
CGAGGGCATT TGGGGGATGA TGGGTTACCT GGATTTCGGG GCCCAAAGGG TGCCCAAGGG 
CTGCCGGGTA TCCCAGGATT CCCAGGCGAG CTAGGAAAGA AAGGGCAAGC AGGACACCCT 
GGGAGAAAGG GTGAACGGGG AGGAGATGGT CATCCTGGCC ACCCAGGAGA CAAAGGGGAC 
TGGGGACCTA AAGGTGAAAA AGGACTTCCT GGGGATGAAG GTAGTATTTC CATGATTTCC 
ACAAAGGGAG AAAAGGGGGA ATTAGGTCAA CCTGGCGAAT ATGGAGTCCC AGGAGAAAGA 
GGAGATAAAG GTTCTTCTGG ACTCCAAGGG GAAGAAGGAT TACCAGGAAG AGATGGACTT 
CCAGGGTTTC AGAAAGGTCA TCCTGGTCCA AGTGGCCAGC CTGGATTTCC AGGACTGCCT 
GGCTTCCCAG GCTCTCCAGG CTCAAGAGGA GTATTTGGTT TCCCTGGACT ACCGGGTGAT 
CCGGGAGATG ATGGTTCACG GGGCATACCT GGACCTTCAG GACCTATTGG AGTCAAAGGC 
CCCAAAGGAA AAAGAGGGGA TTCAATCAGT TTACTTGGCC CACTTGGAGA AAAAGGAAGA 
CCTGGAAACC CTGGATGTGC AGGTCATTTT GGAGCCCCAG GTGACCTGGG TTTGCCTGGG 
CCTCCGGGGT ATCCTGGAAC CCCAGGAAGG CCAGGACCAC CAGGATACCC AGGACAACCT 
GGCTTTCCAG GTACTCAGGG GACCCCTGGA CAAAGAGGAA TTCCAGGAGA AATAGGCGAC 
CCTGGGATCT CAGGACCTAT TGGAGATCAA GGACTTGCTG GGCCCCGAGG CATGAAAGGG 
TTCTCAGGAA TTCCAGGATT AAATGGTTTG CATGGACTCA AGGGGCCAAA AGGAGCGAAG 
GGTTCACCAG GTTTCATCGA AAGAGGACCA CCAGGTTTTG CTGGATTGCC TGGAGCAAAA 
GGGGAAAAAG GAGAACCTGG AAGGGTGGGA CTTTCTTTTC CAGGTCCTTT TGGTGAAAGA 
GGACCTCAAG GTCCCCCAGG GAGGCCTGGA CTTCAGGGAT CTGCAGGTCC TAAAGGAAGA 
ACACCTGAAG GAGAAATTTC TTATCCTGGT CCTCAAGGAG ATATAGGATT TCATGGCCCC 
GATGGTATAC GAGGTCCACC AGGGTCTCCA GGTCAACCTG GAGGTGTTGA CTTTTGGAAA 
GGGAGTCCAG GTGATGATGG CTTTCCAGGA CCACCAGGTC CCTCTGGTCC ACCAGGACAT 
CCTGGACCAA AAGGCATCCC AGGATGCGAA GGAAGAGATG GCCAAAAAGG GCCAATGGGA 
CATCCTGGTC TTCAAGGTCC ACCTGGACGC CCTGGGCCAT ATGGAGATAA AGGGAGTCCA 
GGACCCCCCG GGACTCCTGG GTCACCTGGT CCCAGAGGGG ACCCTGGCCT ACCTGGAGAT 
ATTGCAGATT GTCCCAAAAT CCCCGGGCCC CCAGGGATTC CAGGTTCAAG AGGACCAGAA 
GGACGCATGG GATTTCCCGG TCCAAGAGGA CCCCCAGGCT TACCAGGACT TAAAGGTGAA 
CCAGGGCCAG ATGGCCAGAG AGGTCAAGAT GGCTTTCCTG GATCGCCAGG TGCACCTGGA 
CAAAAGGGAG ACTCAGGGGA ACCAGGCTGT GATGGGCCAC CAGGTCCACC TGGCCCAACT 
GGAGATCCAG GATCCAGAGG GCCAAGAACT GAATACCTTA ATGGATTCCT CCTGGTTCTT 
CATAGTCAGA CAGACAAAAT ACCTTCTTGT CCTGATGGAT TGCCCAAGTT GTGGACTGGG 
TACAGTCTGC TTTATATTGA AGGCCAGGAG AAAGCACATA ATCAAGATCT GGGTCTCGCG 
GGATCCTGTC TTCCCCTGTT CAGCACGATG CCATTTGCTT ACTGCAACAT CAATCACGTC 
TGTTTCTATG CCAGGAGAAA TGATAAATCC TACTGGCTAT CCAGTGCCGC TCCTCTACCC 
ATGATGCCCC TCTCTGAAGA TAAAATACAG CCGTACATTA GCCGCACACA GGAGCTGGAG 
ATCAAGGAGG AGGACAGTCC CTTGTGTCCC CTGGAAGCTG CCTTGAAGAC TTCCGAGCAG 
CCCCCTTCAT TGAATGCCAA GGACGGCAGG GAACGTGTCA TTTTTTTGCC AATGAATACA 
GCTTCTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028924803.1
CDS	222..5033
Translation	MALLRCYFRGIKNISKPWWLIIIFSLPVYGGGKKYTAICGGRNCSACQCFPEKGSRGQPGELGPQGPIGPCGASGPPGLPGEKGMRGDDGSAGAAGERGDKGPTGVPGFPGLDGVPGYPGPPGSRGKPGLDGHNGSRGDPGFPGQHGYPGQVGPAGLPGQKGEKGISAYVAEKGPPGDQGIPGPPGSPGPKGPRGAKGGMGFPGKPGADGPQGLPGLPGAQGNPGVGIKGQTGDPGEVGHQGPPGPPLLVGPPSSCLHKGEKGTKGMPGKSGLPGPVGAKGEPGSGVKGEKGIPGFPGLRGHSGSYGSPGSPGLKGEPGEVGIPGKFGFPGIKGDPGDRGEPGPPGIMVTPPVPLKGLPGDPGVPGPYGEMGSVGKVGPPGPSGKPGEDRAVGVTGPMGSRGAPGVPGPPGEVGVPGKADAPPGKPGNPGYPGTPGARGEQGLPGPDVVHCTSGPPGPQGMKGKAGPPGRSGVKGEKGNEGACACTPGVIGLPGTPGLPGRKGRKGELGYPGRFGEKGDQGSPGAKGYPGPSGIPGSLGMTGPKGEKGDRVIPRIKGRKGERGSQGPPGFPGLQGQPGQDGTEGPVGLPGLPGDRGGAVPGAKGFPGHQGAPGRTGPRGPSGLGFPGRQGMTGIPGDRGTVGDRGPPGAKGQKGDTFPCASDFPGNPGPPGFQGPPGPKGIQGPPGPAGPHGFDGAKGQRGKPGISEVPGPAGFPGDNGDPGFKGDKGTSPVGPQGFPGSPGINGQKGMPGDPAFGHPGLPGNKGSAGFPGPKGFRGDLGYPGSPGQAGTPGLPGTKGFKGQKGHNGPPGSPGPPGYLCEKGSPGLPGQPGLPGPQGNPGYPGLKGQQGDMGAPGPAGMKGLPGAPGLNGSSGPPGPPGNRGHLGDDGLPGFRGPKGAQGLPGIPGFPGELGKKGQAGHPGRKGERGGDGHPGHPGDKGDWGPKGEKGLPGDEGSISMISTKGEKGELGQPGEYGVPGERGDKGSSGLQGEEGLPGRDGLPGFQKGHPGPSGQPGFPGLPGFPGSPGSRGVFGFPGLPGDPGDDGSRGIPGPSGPIGVKGPKGKRGDSISLLGPLGEKGRPGNPGCAGHFGAPGDLGLPGPPGYPGTPGRPGPPGYPGQPGFPGTQGTPGQRGIPGEIGDPGISGPIGDQGLAGPRGMKGFSGIPGLNGLHGLKGPKGAKGSPGFIERGPPGFAGLPGAKGEKGEPGRVGLSFPGPFGERGPQGPPGRPGLQGSAGPKGRTPEGEISYPGPQGDIGFHGPDGIRGPPGSPGQPGGVDFWKGSPGDDGFPGPPGPSGPPGHPGPKGIPGCEGRDGQKGPMGHPGLQGPPGRPGPYGDKGSPGPPGTPGSPGPRGDPGLPGDIADCPKIPGPPGIPGSRGPEGRMGFPGPRGPPGLPGLKGEPGPDGQRGQDGFPGSPGAPGQKGDSGEPGCDGPPGPPGPTGDPGSRGPRTEYLNGFLLVLHSQTDKIPSCPDGLPKLWTGYSLLYIEGQEKAHNQDLGLAGSCLPLFSTMPFAYCNINHVCFYARRNDKSYWLSSAAPLPMMPLSEDKIQPYISRTQELEIKEEDSPLCPLEAALKTSEQPPSLNAKDGRERVIFLPMNTASG

Target ORF information:

RefSeq Version	XM_029068970.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029068970.1

ORF Insert Sequence:

ATGGCGCTAC TAAGATGTTA TTTCAGAGGG ATTAAAAATA TCTCCAAACC CTGGTGGCTC 
ATAATTATTT TTTCTCTACC GGTTTATGGG GGTGGAAAAA AATACACCGC CATTTGTGGA 
GGACGAAATT GTTCAGCTTG CCAATGTTTT CCTGAGAAAG GATCAAGGGG TCAGCCAGGA 
GAGCTTGGAC CACAGGGACC TATCGGACCA TGTGGAGCTT CAGGACCCCC TGGGTTGCCA 
GGAGAGAAAG GAATGAGAGG GGATGATGGA TCTGCGGGAG CAGCTGGAGA AAGGGGTGAT 
AAGGGCCCAA CAGGAGTTCC TGGCTTTCCA GGTTTGGATG GTGTGCCTGG CTACCCAGGA 
CCTCCCGGAT CAAGAGGTAA ACCGGGTTTA GATGGCCATA ATGGTTCAAG AGGAGATCCA 
GGCTTTCCTG GACAACATGG ATACCCAGGA CAAGTTGGCC CAGCTGGTCT ACCTGGACAA 
AAAGGAGAGA AAGGAATCTC AGCATATGTT GCAGAGAAAG GTCCACCTGG AGATCAAGGT 
ATCCCAGGAC CTCCTGGCTC ACCTGGACCA AAGGGCCCAA GGGGAGCAAA GGGCGGTATG 
GGGTTTCCGG GTAAACCAGG TGCAGATGGT CCTCAAGGCC TTCCTGGATT GCCAGGTGCG 
CAGGGCAATC CAGGAGTTGG CATTAAAGGA CAAACTGGAG ATCCAGGAGA AGTTGGACAT 
CAAGGGCCTC CAGGGCCACC CCTACTGGTT GGGCCTCCAA GTTCATGTCT CCACAAAGGA 
GAAAAAGGCA CAAAAGGGAT GCCTGGAAAA AGTGGCCTCC CTGGCCCAGT TGGAGCTAAG 
GGTGAACCTG GAAGTGGGGT GAAGGGAGAG AAAGGTATTC CTGGATTTCC TGGTCTTCGG 
GGTCATTCTG GTTCCTATGG ATCCCCAGGC TCTCCAGGAT TAAAGGGGGA ACCTGGAGAA 
GTTGGCATTC CTGGGAAGTT TGGATTTCCA GGCATAAAGG GAGATCCTGG AGACCGGGGG 
GAACCAGGGC CACCAGGTAT TATGGTAACT CCACCTGTTC CACTAAAAGG ACTTCCAGGA 
GATCCAGGAG TTCCAGGTCC CTATGGAGAA ATGGGATCAG TTGGAAAGGT TGGCCCACCT 
GGTCCTTCAG GTAAACCAGG TGAAGACCGG GCAGTAGGAG TGACAGGGCC AATGGGATCC 
CGAGGAGCAC CGGGTGTTCC AGGTCCCCCA GGGGAAGTTG GTGTTCCTGG AAAAGCAGAT 
GCCCCACCAG GAAAGCCTGG GAATCCAGGA TACCCAGGCA CACCAGGAGC ACGAGGAGAG 
CAGGGGCTGC CTGGACCAGA CGTTGTGCAC TGTACTTCTG GACCTCCTGG ACCTCAAGGA 
ATGAAAGGAA AAGCAGGACC TCCAGGAAGA AGTGGTGTGA AAGGTGAAAA AGGAAATGAA 
GGTGCCTGTG CCTGTACCCC TGGTGTCATT GGCCTACCAG GGACTCCAGG GCTCCCTGGA 
AGAAAAGGAA GGAAAGGGGA GCTGGGATAT CCTGGGAGAT TTGGAGAAAA AGGTGACCAA 
GGTTCTCCCG GGGCAAAAGG ATATCCAGGA CCTTCTGGTA TACCTGGTTC CTTGGGAATG 
ACTGGACCAA AAGGAGAAAA GGGAGACAGA GTTATCCCCA GAATAAAAGG AAGAAAAGGA 
GAAAGAGGCT CCCAAGGACC GCCTGGATTT CCAGGACTTC AAGGACAACC AGGGCAGGAT 
GGAACTGAAG GACCAGTAGG ATTACCTGGA TTGCCAGGTG ATCGAGGAGG CGCAGTTCCA 
GGTGCTAAGG GATTTCCCGG ACACCAAGGA GCCCCTGGGA GGACTGGACC TAGGGGTCCT 
TCGGGTTTGG GGTTTCCTGG ACGACAGGGC ATGACAGGCA TCCCAGGAGA CCGAGGTACC 
GTTGGTGACA GGGGACCACC TGGTGCAAAG GGCCAGAAAG GTGACACATT TCCCTGTGCC 
TCAGATTTTC CTGGAAATCC AGGCCCACCA GGCTTTCAAG GTCCTCCAGG TCCAAAGGGA 
ATTCAAGGAC CCCCAGGTCC AGCTGGACCC CATGGTTTTG ACGGGGCCAA AGGCCAGAGA 
GGCAAGCCAG GAATTTCCGA AGTGCCAGGA CCAGCTGGCT TTCCTGGTGA CAATGGAGAT 
CCAGGTTTTA AAGGTGACAA AGGAACCTCA CCAGTTGGTC CTCAGGGTTT TCCTGGTTCA 
CCTGGTATCA ATGGGCAAAA AGGTATGCCA GGTGATCCTG CTTTTGGCCA CCCAGGACTT 
CCAGGAAACA AAGGTTCTGC AGGATTTCCC GGACCTAAAG GATTTAGAGG TGACCTAGGA 
TATCCAGGAT CTCCAGGACA GGCTGGTACC CCAGGGCTTC CAGGAACCAA AGGATTTAAA 
GGCCAAAAAG GGCATAATGG ACCTCCGGGT TCTCCAGGTC CACCTGGATA CTTATGTGAA 
AAAGGCTCAC CTGGGTTGCC AGGACAACCA GGGCTCCCTG GACCACAAGG TAATCCAGGT 
TACCCTGGCT TGAAAGGCCA GCAAGGAGAT ATGGGTGCCC CTGGACCAGC TGGAATGAAG 
GGTCTTCCAG GAGCACCAGG ATTAAATGGA TCATCTGGTC CCCCAGGACC ACCAGGAAAT 
CGAGGGCATT TGGGGGATGA TGGGTTACCT GGATTTCGGG GCCCAAAGGG TGCCCAAGGG 
CTGCCGGGTA TCCCAGGATT CCCAGGCGAG CTAGGAAAGA AAGGGCAAGC AGGACACCCT 
GGGAGAAAGG GTGAACGGGG AGGAGATGGT CATCCTGGCC ACCCAGGAGA CAAAGGGGAC 
TGGGGACCTA AAGGTGAAAA AGGACTTCCT GGGGATGAAG GTAGTATTTC CATGATTTCC 
ACAAAGGGAG AAAAGGGGGA ATTAGGTCAA CCTGGCGAAT ATGGAGTCCC AGGAGAAAGA 
GGAGATAAAG GTTCTTCTGG ACTCCAAGGG GAAGAAGGAT TACCAGGAAG AGATGGACTT 
CCAGGGTTTC AGAAAGGTCA TCCTGGTCCA AGTGGCCAGC CTGGATTTCC AGGACTGCCT 
GGCTTCCCAG GCTCTCCAGG CTCAAGAGGA GTATTTGGTT TCCCTGGACT ACCGGGTGAT 
CCGGGAGATG ATGGTTCACG GGGCATACCT GGACCTTCAG GACCTATTGG AGTCAAAGGC 
CCCAAAGGAA AAAGAGGGGA TTCAATCAGT TTACTTGGCC CACTTGGAGA AAAAGGAAGA 
CCTGGAAACC CTGGATGTGC AGGTCATTTT GGAGCCCCAG GTGACCTGGG TTTGCCTGGG 
CCTCCGGGGT ATCCTGGAAC CCCAGGAAGG CCAGGACCAC CAGGATACCC AGGACAACCT 
GGCTTTCCAG GTACTCAGGG GACCCCTGGA CAAAGAGGAA TTCCAGGAGA AATAGGCGAC 
CCTGGGATCT CAGGACCTAT TGGAGATCAA GGACTTGCTG GGCCCCGAGG CATGAAAGGG 
TTCTCAGGAA TTCCAGGATT AAATGGTTTG CATGGACTCA AGGGGCCAAA AGGAGCGAAG 
GGTTCACCAG GTTTCATCGA AAGAGGACCA CCAGGTTTTG CTGGATTGCC TGGAGCAAAA 
GGGGAAAAAG GAGAACCTGG AAGGGTGGGA CTTTCTTTTC CAGGTCCTTT TGGTGAAAGA 
GGACCTCAAG GTCCCCCAGG GAGGCCTGGA CTTCAGGGAT CTGCAGGTCC TAAAGGAAGA 
ACACCTGAAG GAGAAATTTC TTATCCTGGT CCTCAAGGAG ATATAGGATT TCATGGCCCC 
GATGGTATAC GAGGTCCACC AGGGTCTCCA GGTCAACCTG GAGGTGTTGA CTTTTGGAAA 
GGGAGTCCAG GTGATGATGG CTTTCCAGGA CCACCAGGTC CCTCTGGTCC ACCAGGACAT 
CCTGGACCAA AAGGCATCCC AGGATGCGAA GGAAGAGATG GCCAAAAAGG GCCAATGGGA 
CATCCTGGTC TTCAAGGTCC ACCTGGACGC CCTGGGCCAT ATGGAGATAA AGGGAGTCCA 
GGACCCCCCG GGACTCCTGG GTCACCTGGT CCCAGAGGGG ACCCTGGCCT ACCTGGAGAT 
ATTGCAGATT GTCCCAAAAT CCCCGGGCCC CCAGGGATTC CAGGTTCAAG AGGACCAGAA 
GGACGCATGG GATTTCCCGG TCCAAGAGGA CCCCCAGGCT TACCAGGACT TAAAGGTGAA 
CCAGGGCCAG ATGGCCAGAG AGGTCAAGAT GGCTTTCCTG GATCGCCAGG TGCACCTGGA 
CAAAAGGGAG ACTCAGGGGA ACCAGGCTGT GATGGGCCAC CAGGTCCACC TGGCCCAACT 
GGAGATCCAG GATCCAGAGG GCCAAGAACT GAATACCTTA ATGGATTCCT CCTGGTTCTT 
CATAGTCAGA CAGACAAAAT ACCTTCTTGT CCTGATGGAT TGCCCAAGTT GTGGACTGGG 
TACAGTCTGC TTTATATTGA AGGCCAGGAG AAAGCACATA ATCAAGATCT GGGTCTCGCG 
GGATCCTGTC TTCCCCTGTT CAGCACGATG CCATTTGCTT ACTGCAACAT CAATCACGTC 
TGTTTCTATG CCAGGAGAAA TGATAAATCC TACTGGCTAT CCAGTGCCGC TCCTCTACCC 
ATGATGCCCC TCTCTGAAGA TAAAATACAG CCGTACATTA GCCGCACACA GGAGCTGGAG 
ATCAAGGAGG AGGACAGTCC CTTGTGTCCC CTGGAAGCTG CCTTGAAGAC TTCCGAGCAG 
CCCCCTTCAT TGAATGCCAA GGACGGCAGG GAACGTGTCA TTTTTTTGCC AATGAATACA 
GCTTCTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_007669913.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007668103.1 Ornithorhynchus anatinus collagen type IV alpha 4 (COL4A4), mRNA.

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CloneID	OOc02117
Clone ID Related Accession (Same CDS sequence)	XM_007669913.1
Accession Version	XM_007669913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	collagen alpha-4(IV) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009100.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGGCTGCTC TTTCAGCAAA AGGGAACCTC TACTTAGATA TCAGACATAA AAGAATGAGT 
TTGCATGAAA AACAAATAGT TTCAGAGGGG TGTAACTTTG ACGGCCCAGC CAGCCTTGAG 
GTGCTGGAAG AGATTGGGGA CATGAAGCTG GGACTGCACA AGGATCCCAT GACCCCCATC 
CAAGACATTT TTGTCAGTGA CAGCCATCTG GACTTTTGTA ATGCTCTCCG TAAACATCAC 
GGCACTATAA AGAATTTTCC CATCAACCTG GTGGTCCAGT CCGAGCTTGA TGTGGCCACT 
GTGTCTCATG GGTGGCTCAT AATTATTTTT TCTCTACCGG TTTATGGGGG TGGAAAAAAA 
TACACCGCCA TTTGTGGAGG ACGAAATTGT TCAGCTTGCC AATGTTTTCC TGAGAAAGGA 
TCAAGGGGTC AGCCAGGAGA GCTTGGACCA CAGGGACCTA TCGGACCATG TGGAGCTTCA 
GGACCCCCTG GGTTGCCAGG AGAGAAAGGA ATGAGAGGGG ATGATGGATC TGCGGGAGCA 
GCTGGAGAAA GGGGTGATAA GGGCCCAACA GGAGTTCCTG GCTTTCCAGG TTTGGATGGT 
GTGCCTGGCT ACCCAGGACC TCCCGGATCA AGAGGTAAAC CGGGTTTAGA TGGCCATAAT 
GGTTCAAGAG GAGATCCAGG CTTTCCTGGA CAACATGGAT ACCCAGGACA AGTTGGCCCA 
GCTGGTCTAC CTGGACAAAA AGGAGAGAAA GGAATCTCAG CATATGTTGC AGAGAAAGGT 
CCACCTGGAG ATCAAGGTAT CCCAGGACCT CCTGGCTCAC CTGGACCAAA GGGCCCAAGG 
GGAGCAAAGG GCGGTATGGG GTTTCCGGGT AAACCAGGTG CAGATGGTCC TCAAGGCCTT 
CCTGGATTGC CAGGTGCGCA GGGCAATCCA GGAGTTGGCA TTAAAGGACA AACTGGAGAT 
CCAGGAGAAG TTGGACATCA AGGGCCTCCA GGGCCACCCC TACTGGTTGG GCCTCCAAGT 
TCATGTCTCC ACAAAGGAGA AAAAGGCACA AAAGGGATGC CTGGAAAAAG TGGCCTCCCT 
GGCCCAGTTG GAGCTAAGGG TGAACCTGGA AGTGGGGTGA AGGGAGAGAA AGGTATTCCT 
GGATTTCCTG GTCTTCGGGG TCATTCTGGT TCCTATGGAT CCCCAGGCTC TCCAGGATTA 
AAGGGGGAAC CTGGAGAAGT TGGCATTCCT GGGAAGTTTG GATTTCCAGG CATAAAGGGA 
GATCCTGGAG ACCGGGGGGA ACCAGGGCCA CCAGGTATTA TGGTAACTCC ACCTGTTCCA 
CTAAAAGGAC TTCCAGGAGA TCCAGGAGTT CCAGGTCCCT ATGGAGAAAT GGGATCAGTT 
GGAAAGGTTG GCCCACCTGG TCCTTCAGGT AAACCAGGTG AAGACCGGGC AGGAGTGACA 
GGGCCAATGG GATCCCGAGG AGCACCGGGT GTTCCAGGTC CCCCAGGGGA AGTTGGTGTT 
CCTGGAAAAG CAGATGCCCC ACCAGGAAAG CCTGGGAATC CAGGATACCC AGGCACACCA 
GGAGCACGAG GAGAGCAGGG GCTGCCTGGA CCAGACGTTG TGCACTGTAC TTCTGGACCT 
CCTGGACCTC AAGGAATGAA AGGAAAAGCA GGACCTCCAG GAAGAAGTGG TGTGAAAGGT 
GAAAAAGGAA ATGAAGGTGC CTGTGCCTGT ACCCCTGGTG TCATTGGCCT ACCAGGGACT 
CCAGGGCTCC CTGGAAGAAA AGGAAGGAAA GGGGAGCTGG GATATCCTGG GAGATTTGGA 
GAAAAAGGTG ACCAAGGTTC TCCCGGGGCA AAAGGATATC CAGGACCTTC TGGTATACCT 
GGTTCCTTGG GAATGACTGG ACCAAAAGGA GAAAAGGGAG ACAGAGTTAT CCCCAGAATA 
AAAGGAAGAA AAGGAGAAAG AGGCTCCCAA GGACCGCCTG GATTTCCAGG ACTTCAAGGA 
CAACCAGGGC AGGATGGAAC TGAAGGACCA GTAGGATTAC CTGGATTGCC AGGTGATCGA 
GGAGGCGCAG TTCCAGGTGC TAAGGGATTT CCCGGACACC AAGGAGCCCC TGGGAGGACT 
GGACCTAGGG GTCCTTCGGG TTTGGGGTTT CCTGGACGAC AGGGCATGAC AGGCATCCCA 
GGAGACCGAG GTACCGTTGG TGACAGGGGA CCACCTGGTG CAAAGGGCCA GAAAGGTGAC 
ACATTTCCCT GTGCCTCAGA TTTTCCTGGA AATCCAGGCC CACCAGGCTT TCAAGGTCCT 
CCAGGTCCAA AGGGAATTCA AGGACCCCCA GGTCCAGCTG GACCCCATGG TTTTGACGGG 
GCCAAAGGCC AGAGAGGCAA GCCAGGAATT TCCGAAGTGC CAGGACCAGC TGGCTTTCCT 
GGTGACAATG GAGATCCAGG TTTTAAAGGT GACAAAGGAA CCTCACCAGT TGGTCCTCAG 
GGTTTTCCTG GTTCACCTGG TATCAATGGG CAAAAAGGTA TGCCAGGTGA TCCTGCTTTT 
GGCCACCCAG GACTTCCAGG AAACAAAGGT TCTGCAGGAT TTCCCGGACC TAAAGGATTT 
AGAGGTGACC TAGGATATCC AGGATCTCCA GGACAGGCTG GTACCCCAGG GCTTCCAGGA 
ACCAAAGGAT TTAAAGGCCA AAAAGGGCAT AATGGACCTC CGGGTTCTCC AGGTCCACCT 
GGATACTTAT GTGAAAAAGG CTCACCTGGG TTGCCAGGAC AACCAGGGCT CCCTGGACCA 
CAAGGTAATC CAGGTTACCC TGGCTTGAAA GGCCAGCAAG GAGATATGGG TGCCCCTGGA 
CCAGCTGGAA TGAAGGGTCT TCCAGGAGCA CCAGGATTAA ATGGATCATC TGGTCCCCCA 
GGACCACCAG GAAATCGAGG GCATTTGGGG GATGATGGGT TACCTGGATT TCGGGGCCCA 
AAGGGTGCCC AAGGGCTGCC GGGTATCCCA GGATTCCCAG GCGAGCTAGG AAAGAAAGGG 
CAAGCAGGAC ACCCTGGGAG AAAGGGTGAA CGGGGAGGAG ATGGTCATCC TGGCCACCCA 
GGAGACAAAG GGGACTGGGG ACCTAAAGGT GAAAAAGGAC TTCCTGGGGA TGAAGGTAGT 
ATTTCCATGA TTTCCACAAA GGGAGAAAAG GGGGAATTAG GTCAACCTGG CGAATATGGA 
GTCCCAGGAG AAAGAGGAGA TAAAGGTTCT TCTGGACTCC AAGGGGAAGA AGGATTACCA 
GGAAGAGATG GACTTCCAGG GTTTCAGAAA GGTCATCCTG GTCCAAGTGG CCAGCCTGGA 
TTTCCAGGAC TGCCTGGCTT CCCAGGCTCT CCAGGTTCAA GAGGAGTATT TGGTTTCCCT 
GGACTACCGG GTGATCCGGG AGATGATGGT TCACGGGGCA TACCTGGACC TTCAGGACCT 
ATTGGAGTCA AAGGCCCCAA AGGAAAAAGA GGGGATTCAA TCAGTTTACT TGGCCCACTT 
GGAGAAAAAG GAAGACCTGG AAACCCTGGA TGTGCAGGTC ATTTTGGAGC CCCAGGTGAC 
CTGGGTTTGC CTGGGCCTCC GGGGTATCCT GGAACCCCAG GAAGGCCAGG ACCACCAGGA 
TACCCAGGAC AACCTGGCTT TCCAGGTACT CAGGGGACCC CTGGACAAAG AGGAATTCCA 
GGAGAAATAG GCGACCCTGG GATCTCAGGA CCTATTGGAG ATCAAGGACT TGCTGGGCCC 
CGAGGCATGA AAGGGTTCTC AGGAATTCCA GGATTAAATG GTTTGCATGG ACTCAAGGGG 
CCAAAAGGAG CGAAGGGTTC ACCAGGTTTC ATCGAAAGAG GACCACCAGG TTTTTCTGGA 
TTGCCTGGAT CAAAAGGGGA AAAAGGAGAA CCTGGAAGGG TGGGACTTTC TTTTCCAGGT 
CCTTTTGGTG AAAGAGGACC TCAAGGTCCC CCAGGGAGGC CTGGACTTCA GGGATCTGCA 
GGTCCTAAAG GAAGAACACC TGAAGGAGAA ATTTCTTATC CTGGTCCTCA AGGAGATATA 
GGATTTCATG GCCCCGATGG TATACGAGGT CCACCAGGGT CTCCAGGTCA ACCTGGAGGT 
GTTGACTTTT GGAAAGGGAG TCCAGGTGAT GATGGCTTTC CAGGACCACC AGGTCCCTCT 
GGTCCACCAG GACATCCTGG ACCAAAAGGC ATCCCAGGAT GCAAAGGAAG AGATGGCCAA 
AAAGGGCCAA TGGGACATCC TGGTCTTCAA GGTCCACCTG GACGCCCTGG GCCATATGGA 
GATAAAGGGA GTCCAGGACC CCCGGGGACT CCTGGGTCAC CTGGTCCCAG AGGGGACCCT 
GGCCTACCTG GAGATATTGC AGATTGTCCC AAAATCCCCG GGCCCCCAGG GATTCCAGGT 
TCAAGAGGAC CAGAAGGACG CATGGGATTT CCCGGTCCAA GAGGACCCCC AGGCTTACCA 
GGACTTAAAG GTGAACCAGG GCCAGATGGC CAGAGAGGTC AAGATGGCTT TCCTGGATCG 
CCAGGTGCAC CTGGACAAAA GGGAGACTCA GGGGAACCAG GCTGTGATGG GCCACCAGGT 
CCACCTGGCC CAACTGGAGA TCCAGGATCC AGAGGGCCAA GAACTGAATA CCTTAATGGA 
TTCCTCCTGG TTCTTCATAG TCAGACAGAC AAAATACCTT CTTGTCCTGA TGGATTGCCC 
AAGTTGTGGA CTGGGTACAG TCTGCTTTAT ATTGAAGGCC AGGAGAAAGC ACATAATCAA 
GATCTGGGTC TCGCGGGATC CTGTCTTCCC CTGTTCAGCA CGATGCCATT TGCTTACTGC 
AACATCAATC ACGTCTGTTT CTATGCCAGG AGAAATGATA AATCCTACTG GCTATCCAGT 
GCCGCTCCTC TACCCATGAT GCCCCTCTCT GAAGATAAAA TACAGCCGTA CATTAGCCGG 
TGTGTGGTAT GCGAGTCCCC GGCCCAGGCC TTGGCCGTTC ACAGCCAAGA TGAATCCATC 
CCTCCATGTC CGACATCATG GAGAAGCCTC TGGATAGGTT ATTCTTTCCT AATGCACACA 
GGAGCTGGAG ATCAAGGAGG AGGACAGTCC CTTGTGTCCC CTGGAAGCTG CCTTGAAGAC 
TTCCGAGCAG CCCCCTTCAT TGAATGCCAA GGACGGCAGG GAACGTGTCA TTTTTTTGCC 
AATGAATACA GCTTCTGGCT AACGACAGTG AAACCAGAAT TACAGTTTTC ATCAGCTCCC 
TCACCAAGCA CCTTAAAAGA TGGACAGGCA CAGCGCCAAA AAATCAGCAG ATGTCAGGTC 
TGCATGAAAT ACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007668103.1
CDS	1..5298
Translation	MAALSAKGNLYLDIRHKRMSLHEKQIVSEGCNFDGPASLEVLEEIGDMKLGLHKDPMTPIQDIFVSDSHLDFCNALRKHHGTIKNFPINLVVQSELDVATVSHGWLIIIFSLPVYGGGKKYTAICGGRNCSACQCFPEKGSRGQPGELGPQGPIGPCGASGPPGLPGEKGMRGDDGSAGAAGERGDKGPTGVPGFPGLDGVPGYPGPPGSRGKPGLDGHNGSRGDPGFPGQHGYPGQVGPAGLPGQKGEKGISAYVAEKGPPGDQGIPGPPGSPGPKGPRGAKGGMGFPGKPGADGPQGLPGLPGAQGNPGVGIKGQTGDPGEVGHQGPPGPPLLVGPPSSCLHKGEKGTKGMPGKSGLPGPVGAKGEPGSGVKGEKGIPGFPGLRGHSGSYGSPGSPGLKGEPGEVGIPGKFGFPGIKGDPGDRGEPGPPGIMVTPPVPLKGLPGDPGVPGPYGEMGSVGKVGPPGPSGKPGEDRAGVTGPMGSRGAPGVPGPPGEVGVPGKADAPPGKPGNPGYPGTPGARGEQGLPGPDVVHCTSGPPGPQGMKGKAGPPGRSGVKGEKGNEGACACTPGVIGLPGTPGLPGRKGRKGELGYPGRFGEKGDQGSPGAKGYPGPSGIPGSLGMTGPKGEKGDRVIPRIKGRKGERGSQGPPGFPGLQGQPGQDGTEGPVGLPGLPGDRGGAVPGAKGFPGHQGAPGRTGPRGPSGLGFPGRQGMTGIPGDRGTVGDRGPPGAKGQKGDTFPCASDFPGNPGPPGFQGPPGPKGIQGPPGPAGPHGFDGAKGQRGKPGISEVPGPAGFPGDNGDPGFKGDKGTSPVGPQGFPGSPGINGQKGMPGDPAFGHPGLPGNKGSAGFPGPKGFRGDLGYPGSPGQAGTPGLPGTKGFKGQKGHNGPPGSPGPPGYLCEKGSPGLPGQPGLPGPQGNPGYPGLKGQQGDMGAPGPAGMKGLPGAPGLNGSSGPPGPPGNRGHLGDDGLPGFRGPKGAQGLPGIPGFPGELGKKGQAGHPGRKGERGGDGHPGHPGDKGDWGPKGEKGLPGDEGSISMISTKGEKGELGQPGEYGVPGERGDKGSSGLQGEEGLPGRDGLPGFQKGHPGPSGQPGFPGLPGFPGSPGSRGVFGFPGLPGDPGDDGSRGIPGPSGPIGVKGPKGKRGDSISLLGPLGEKGRPGNPGCAGHFGAPGDLGLPGPPGYPGTPGRPGPPGYPGQPGFPGTQGTPGQRGIPGEIGDPGISGPIGDQGLAGPRGMKGFSGIPGLNGLHGLKGPKGAKGSPGFIERGPPGFSGLPGSKGEKGEPGRVGLSFPGPFGERGPQGPPGRPGLQGSAGPKGRTPEGEISYPGPQGDIGFHGPDGIRGPPGSPGQPGGVDFWKGSPGDDGFPGPPGPSGPPGHPGPKGIPGCKGRDGQKGPMGHPGLQGPPGRPGPYGDKGSPGPPGTPGSPGPRGDPGLPGDIADCPKIPGPPGIPGSRGPEGRMGFPGPRGPPGLPGLKGEPGPDGQRGQDGFPGSPGAPGQKGDSGEPGCDGPPGPPGPTGDPGSRGPRTEYLNGFLLVLHSQTDKIPSCPDGLPKLWTGYSLLYIEGQEKAHNQDLGLAGSCLPLFSTMPFAYCNINHVCFYARRNDKSYWLSSAAPLPMMPLSEDKIQPYISRCVVCESPAQALAVHSQDESIPPCPTSWRSLWIGYSFLMHTGAGDQGGGQSLVSPGSCLEDFRAAPFIECQGRQGTCHFFANEYSFWLTTVKPELQFSSAPSPSTLKDGQAQRQKISRCQVCMKYS

Target ORF information:

RefSeq Version	XM_007669913.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus collagen type IV alpha 4 (COL4A4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007669913.1

ORF Insert Sequence:

ATGGCTGCTC TTTCAGCAAA AGGGAACCTC TACTTAGATA TCAGACATAA AAGAATGAGT 
TTGCATGAAA AACAAATAGT TTCAGAGGGG TGTAACTTTG ACGGCCCAGC CAGCCTTGAG 
GTGCTGGAAG AGATTGGGGA CATGAAGCTG GGACTGCACA AGGATCCCAT GACCCCCATC 
CAAGACATTT TTGTCAGTGA CAGCCATCTG GACTTTTGTA ATGCTCTCCG TAAACATCAC 
GGCACTATAA AGAATTTTCC CATCAACCTG GTGGTCCAGT CCGAGCTTGA TGTGGCCACT 
GTGTCTCATG GGTGGCTCAT AATTATTTTT TCTCTACCGG TTTATGGGGG TGGAAAAAAA 
TACACCGCCA TTTGTGGAGG ACGAAATTGT TCAGCTTGCC AATGTTTTCC TGAGAAAGGA 
TCAAGGGGTC AGCCAGGAGA GCTTGGACCA CAGGGACCTA TCGGACCATG TGGAGCTTCA 
GGACCCCCTG GGTTGCCAGG AGAGAAAGGA ATGAGAGGGG ATGATGGATC TGCGGGAGCA 
GCTGGAGAAA GGGGTGATAA GGGCCCAACA GGAGTTCCTG GCTTTCCAGG TTTGGATGGT 
GTGCCTGGCT ACCCAGGACC TCCCGGATCA AGAGGTAAAC CGGGTTTAGA TGGCCATAAT 
GGTTCAAGAG GAGATCCAGG CTTTCCTGGA CAACATGGAT ACCCAGGACA AGTTGGCCCA 
GCTGGTCTAC CTGGACAAAA AGGAGAGAAA GGAATCTCAG CATATGTTGC AGAGAAAGGT 
CCACCTGGAG ATCAAGGTAT CCCAGGACCT CCTGGCTCAC CTGGACCAAA GGGCCCAAGG 
GGAGCAAAGG GCGGTATGGG GTTTCCGGGT AAACCAGGTG CAGATGGTCC TCAAGGCCTT 
CCTGGATTGC CAGGTGCGCA GGGCAATCCA GGAGTTGGCA TTAAAGGACA AACTGGAGAT 
CCAGGAGAAG TTGGACATCA AGGGCCTCCA GGGCCACCCC TACTGGTTGG GCCTCCAAGT 
TCATGTCTCC ACAAAGGAGA AAAAGGCACA AAAGGGATGC CTGGAAAAAG TGGCCTCCCT 
GGCCCAGTTG GAGCTAAGGG TGAACCTGGA AGTGGGGTGA AGGGAGAGAA AGGTATTCCT 
GGATTTCCTG GTCTTCGGGG TCATTCTGGT TCCTATGGAT CCCCAGGCTC TCCAGGATTA 
AAGGGGGAAC CTGGAGAAGT TGGCATTCCT GGGAAGTTTG GATTTCCAGG CATAAAGGGA 
GATCCTGGAG ACCGGGGGGA ACCAGGGCCA CCAGGTATTA TGGTAACTCC ACCTGTTCCA 
CTAAAAGGAC TTCCAGGAGA TCCAGGAGTT CCAGGTCCCT ATGGAGAAAT GGGATCAGTT 
GGAAAGGTTG GCCCACCTGG TCCTTCAGGT AAACCAGGTG AAGACCGGGC AGGAGTGACA 
GGGCCAATGG GATCCCGAGG AGCACCGGGT GTTCCAGGTC CCCCAGGGGA AGTTGGTGTT 
CCTGGAAAAG CAGATGCCCC ACCAGGAAAG CCTGGGAATC CAGGATACCC AGGCACACCA 
GGAGCACGAG GAGAGCAGGG GCTGCCTGGA CCAGACGTTG TGCACTGTAC TTCTGGACCT 
CCTGGACCTC AAGGAATGAA AGGAAAAGCA GGACCTCCAG GAAGAAGTGG TGTGAAAGGT 
GAAAAAGGAA ATGAAGGTGC CTGTGCCTGT ACCCCTGGTG TCATTGGCCT ACCAGGGACT 
CCAGGGCTCC CTGGAAGAAA AGGAAGGAAA GGGGAGCTGG GATATCCTGG GAGATTTGGA 
GAAAAAGGTG ACCAAGGTTC TCCCGGGGCA AAAGGATATC CAGGACCTTC TGGTATACCT 
GGTTCCTTGG GAATGACTGG ACCAAAAGGA GAAAAGGGAG ACAGAGTTAT CCCCAGAATA 
AAAGGAAGAA AAGGAGAAAG AGGCTCCCAA GGACCGCCTG GATTTCCAGG ACTTCAAGGA 
CAACCAGGGC AGGATGGAAC TGAAGGACCA GTAGGATTAC CTGGATTGCC AGGTGATCGA 
GGAGGCGCAG TTCCAGGTGC TAAGGGATTT CCCGGACACC AAGGAGCCCC TGGGAGGACT 
GGACCTAGGG GTCCTTCGGG TTTGGGGTTT CCTGGACGAC AGGGCATGAC AGGCATCCCA 
GGAGACCGAG GTACCGTTGG TGACAGGGGA CCACCTGGTG CAAAGGGCCA GAAAGGTGAC 
ACATTTCCCT GTGCCTCAGA TTTTCCTGGA AATCCAGGCC CACCAGGCTT TCAAGGTCCT 
CCAGGTCCAA AGGGAATTCA AGGACCCCCA GGTCCAGCTG GACCCCATGG TTTTGACGGG 
GCCAAAGGCC AGAGAGGCAA GCCAGGAATT TCCGAAGTGC CAGGACCAGC TGGCTTTCCT 
GGTGACAATG GAGATCCAGG TTTTAAAGGT GACAAAGGAA CCTCACCAGT TGGTCCTCAG 
GGTTTTCCTG GTTCACCTGG TATCAATGGG CAAAAAGGTA TGCCAGGTGA TCCTGCTTTT 
GGCCACCCAG GACTTCCAGG AAACAAAGGT TCTGCAGGAT TTCCCGGACC TAAAGGATTT 
AGAGGTGACC TAGGATATCC AGGATCTCCA GGACAGGCTG GTACCCCAGG GCTTCCAGGA 
ACCAAAGGAT TTAAAGGCCA AAAAGGGCAT AATGGACCTC CGGGTTCTCC AGGTCCACCT 
GGATACTTAT GTGAAAAAGG CTCACCTGGG TTGCCAGGAC AACCAGGGCT CCCTGGACCA 
CAAGGTAATC CAGGTTACCC TGGCTTGAAA GGCCAGCAAG GAGATATGGG TGCCCCTGGA 
CCAGCTGGAA TGAAGGGTCT TCCAGGAGCA CCAGGATTAA ATGGATCATC TGGTCCCCCA 
GGACCACCAG GAAATCGAGG GCATTTGGGG GATGATGGGT TACCTGGATT TCGGGGCCCA 
AAGGGTGCCC AAGGGCTGCC GGGTATCCCA GGATTCCCAG GCGAGCTAGG AAAGAAAGGG 
CAAGCAGGAC ACCCTGGGAG AAAGGGTGAA CGGGGAGGAG ATGGTCATCC TGGCCACCCA 
GGAGACAAAG GGGACTGGGG ACCTAAAGGT GAAAAAGGAC TTCCTGGGGA TGAAGGTAGT 
ATTTCCATGA TTTCCACAAA GGGAGAAAAG GGGGAATTAG GTCAACCTGG CGAATATGGA 
GTCCCAGGAG AAAGAGGAGA TAAAGGTTCT TCTGGACTCC AAGGGGAAGA AGGATTACCA 
GGAAGAGATG GACTTCCAGG GTTTCAGAAA GGTCATCCTG GTCCAAGTGG CCAGCCTGGA 
TTTCCAGGAC TGCCTGGCTT CCCAGGCTCT CCAGGTTCAA GAGGAGTATT TGGTTTCCCT 
GGACTACCGG GTGATCCGGG AGATGATGGT TCACGGGGCA TACCTGGACC TTCAGGACCT 
ATTGGAGTCA AAGGCCCCAA AGGAAAAAGA GGGGATTCAA TCAGTTTACT TGGCCCACTT 
GGAGAAAAAG GAAGACCTGG AAACCCTGGA TGTGCAGGTC ATTTTGGAGC CCCAGGTGAC 
CTGGGTTTGC CTGGGCCTCC GGGGTATCCT GGAACCCCAG GAAGGCCAGG ACCACCAGGA 
TACCCAGGAC AACCTGGCTT TCCAGGTACT CAGGGGACCC CTGGACAAAG AGGAATTCCA 
GGAGAAATAG GCGACCCTGG GATCTCAGGA CCTATTGGAG ATCAAGGACT TGCTGGGCCC 
CGAGGCATGA AAGGGTTCTC AGGAATTCCA GGATTAAATG GTTTGCATGG ACTCAAGGGG 
CCAAAAGGAG CGAAGGGTTC ACCAGGTTTC ATCGAAAGAG GACCACCAGG TTTTTCTGGA 
TTGCCTGGAT CAAAAGGGGA AAAAGGAGAA CCTGGAAGGG TGGGACTTTC TTTTCCAGGT 
CCTTTTGGTG AAAGAGGACC TCAAGGTCCC CCAGGGAGGC CTGGACTTCA GGGATCTGCA 
GGTCCTAAAG GAAGAACACC TGAAGGAGAA ATTTCTTATC CTGGTCCTCA AGGAGATATA 
GGATTTCATG GCCCCGATGG TATACGAGGT CCACCAGGGT CTCCAGGTCA ACCTGGAGGT 
GTTGACTTTT GGAAAGGGAG TCCAGGTGAT GATGGCTTTC CAGGACCACC AGGTCCCTCT 
GGTCCACCAG GACATCCTGG ACCAAAAGGC ATCCCAGGAT GCAAAGGAAG AGATGGCCAA 
AAAGGGCCAA TGGGACATCC TGGTCTTCAA GGTCCACCTG GACGCCCTGG GCCATATGGA 
GATAAAGGGA GTCCAGGACC CCCGGGGACT CCTGGGTCAC CTGGTCCCAG AGGGGACCCT 
GGCCTACCTG GAGATATTGC AGATTGTCCC AAAATCCCCG GGCCCCCAGG GATTCCAGGT 
TCAAGAGGAC CAGAAGGACG CATGGGATTT CCCGGTCCAA GAGGACCCCC AGGCTTACCA 
GGACTTAAAG GTGAACCAGG GCCAGATGGC CAGAGAGGTC AAGATGGCTT TCCTGGATCG 
CCAGGTGCAC CTGGACAAAA GGGAGACTCA GGGGAACCAG GCTGTGATGG GCCACCAGGT 
CCACCTGGCC CAACTGGAGA TCCAGGATCC AGAGGGCCAA GAACTGAATA CCTTAATGGA 
TTCCTCCTGG TTCTTCATAG TCAGACAGAC AAAATACCTT CTTGTCCTGA TGGATTGCCC 
AAGTTGTGGA CTGGGTACAG TCTGCTTTAT ATTGAAGGCC AGGAGAAAGC ACATAATCAA 
GATCTGGGTC TCGCGGGATC CTGTCTTCCC CTGTTCAGCA CGATGCCATT TGCTTACTGC 
AACATCAATC ACGTCTGTTT CTATGCCAGG AGAAATGATA AATCCTACTG GCTATCCAGT 
GCCGCTCCTC TACCCATGAT GCCCCTCTCT GAAGATAAAA TACAGCCGTA CATTAGCCGG 
TGTGTGGTAT GCGAGTCCCC GGCCCAGGCC TTGGCCGTTC ACAGCCAAGA TGAATCCATC 
CCTCCATGTC CGACATCATG GAGAAGCCTC TGGATAGGTT ATTCTTTCCT AATGCACACA 
GGAGCTGGAG ATCAAGGAGG AGGACAGTCC CTTGTGTCCC CTGGAAGCTG CCTTGAAGAC 
TTCCGAGCAG CCCCCTTCAT TGAATGCCAA GGACGGCAGG GAACGTGTCA TTTTTTTGCC 
AATGAATACA GCTTCTGGCT AACGACAGTG AAACCAGAAT TACAGTTTTC ATCAGCTCCC 
TCACCAAGCA CCTTAAAAGA TGGACAGGCA CAGCGCCAAA AAATCAGCAG ATGTCAGGTC 
TGCATGAAAT ACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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COL4A4 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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