LOC112290175 cDNA ORF clone,

Gene
Clones
gRNAs

The following LOC112290175 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC112290175 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPn55192	XM_024536196.1 Latest version!	Physcomitrella patens heat shock 70 kDa protein 12A-like (LOC112290175), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC112290175 ( XM_024536196.1 ) cDNA ORF clone, -> XP_024391964.1 Physcomitrella patens heat shock 70 kDa protein 12A-like (LOC112290175), mRNA.

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CloneID	OPn55192
Clone ID Related Accession (Same CDS sequence)	XM_024536196.1
Accession Version	XM_024536196.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	heat shock 70 kDa protein 12A-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037265.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTCT TGAGTTGGGG CTCATCGCTG AAAAGAGAAA AATGCCCTGT GGAACGGGTA 
CGGGGTAATG AATCAAACGG AGTCGGAAAA ATAGGACCGT CGTCATCGAG AGGCTATAAC 
GATCGAGTAA TTGTCGAAGC TGCAAACCCT GGTCCGCTCC GAATCAGCAG GAATCTTCCT 
AGTGCTGTAC CAGGCACTAG CGTGGAGGAA GTGTCGGGTG CCGTTCGCAT TCCTGACGAG 
GTCAGATCTA CGCCTCAAAA TGGTTGGGTG ATAGGGTTAG ATTTCGGAAC CACATTCTCA 
GGGTTTGCCT ACGCCAAGGT TTCAGATCCT GAGCACATCT ATGTCCATCA CGACTGGCCC 
TGCATGAGCG GTGGGAACGA ATATTGCAAG ACATTGACGG GCCTTTTCTA CACAAAGACG 
GCGACTGGGG AATTGCAGTG TACGTCTTGG GGTCACCCAG CTCGATCCGA TTATATGGCG 
AATCGCGGGG ATAGTTCCAC CCGAGGATTT TACCTGTCAA AGTTCAAGCT GCTTCTGAAG 
AAAGATCTGG ACGTTTCTTC CCTGGCGAGT GCCATACCGC CGTCACTTAC AGTGAGCAAA 
ATCATCACAC AATATTTGAA GCACATCGGC GAGTATGCCT TATCCGTACT CAGGAACCAT 
GATGGAGAGG TGAGGTTCAG CCAAGACTCA GTGCAATGGT GCGTGACAGT GCCTTCAATT 
TGGGACGATG CGGCGAAGCA GCAGATGAAG GTGTGCATGA TGGACACGGG GCTTGTATCT 
TGCGATAGCG GAATAGAGTC GGTGAAAGTT GTCTTGGAAC CAGAAGCAGC TTCCTTCCAC 
TGCCACCAAC TTATGCCAAA GCAATACCCC AAAGTGTGCT TAGACAGATG TGATAATATT 
CTGGTGGCCG ACATCGGGGG TGGGACAGTC GACATCGTGG TGCAGAAGTT GAAAGGCATC 
GGGAGGGATT ACAAAGTTGA GGAGCTCACA GAAAGCTCTG GTGGTCTTTG CGGTGGGACA 
ATTGTTGACG ATTATTTCTT GCGGTTCGTG TCCAAACGAA TAGGATGCCC GGAAGAATTT 
CTGCGGTTGG ATGCCCAAAA CTGCAGGTCG CGCTTTCTCA AGGATTGGGA AGAAATCAAG 
CGTGGGTTCG GCCATGAGAT GAGGCCGACT ACGAATACAG AAATTTCACT GCACCGTAAC 
TTGGCTGAAA AGTGGGAGGA TTACGAGAGG AGACGAGGGT ATCCACCGCG AAAGTCATAT 
AGCGAAATTA AACTCACACA GCAGGATATG ATGTCCATTT TCGATCCAGT GGTGGATGAG 
ATTATCAAAC TGATTGCTGC CCAGCTAGAG AAAGTGACCA ACATCAAGGC GATATTCGTG 
GTTGGTGGAT TCGCAGGCTC TCAGTATCTA ATGCGACAAA TCCGAGCGCG TTTTTCTCGT 
CCAGAGACGC ATATCCTCAG TCCTCCTCAC CCTGGAAGCG CCATCGTTAA AGGTGCCGTT 
GCCCTGGCAC TCAATCCCGG GGTCGTTGTG GCTCGAATTC TTAAGAGAAC TTACGGCACC 
AGTCTAACCA AGCGATTAGA GCCAGGTGTT GACCCTAGGC ACTTACTGAA AGTCGCCATT 
GACGGGTCGC AGTGGTGTCG CAAGAGATTC GACATTTTCG CGAAGAAAGG GACTCGGATG 
GAGATCAACA ACTGTGTAAA GAAGGAATAC GCACCATACA AAAGCGGCCA ATCACACATC 
AAGTTCGATC TGCACAGTTC AGGTGACGAG GAACCCAGGT ACACAGATGA CCCCGGCGTT 
GTTAGCGAGG GAGAGTTCAC CGTCGACTTA CCCAAGAACC CCACACACGA CAACGTGCCG 
GTCTACGAAT TTTCTTTGTA CTTCGGGCGC AGCAGCATCG AACTTCGAGA AGAGGCCAAG 
TTCCCACACA TCCACGAGGC AGAGCGACGG GTGTATCGTA GTTGGCTCGA AAAAATGAAA 
AAGAAACAAA TCCAGGGGGA GAGCACGTAC ACCTTAGAAC TGACTGTCTC CGACTACACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024391964.1
CDS	1442..3484
Translation	MAFLSWGSSLKREKCPVERVRGNESNGVGKIGPSSSRGYNDRVIVEAANPGPLRISRNLPSAVPGTSVEEVSGAVRIPDEVRSTPQNGWVIGLDFGTTFSGFAYAKVSDPEHIYVHHDWPCMSGGNEYCKTLTGLFYTKTATGELQCTSWGHPARSDYMANRGDSSTRGFYLSKFKLLLKKDLDVSSLASAIPPSLTVSKIITQYLKHIGEYALSVLRNHDGEVRFSQDSVQWCVTVPSIWDDAAKQQMKVCMMDTGLVSCDSGIESVKVVLEPEAASFHCHQLMPKQYPKVCLDRCDNILVADIGGGTVDIVVQKLKGIGRDYKVEELTESSGGLCGGTIVDDYFLRFVSKRIGCPEEFLRLDAQNCRSRFLKDWEEIKRGFGHEMRPTTNTEISLHRNLAEKWEDYERRRGYPPRKSYSEIKLTQQDMMSIFDPVVDEIIKLIAAQLEKVTNIKAIFVVGGFAGSQYLMRQIRARFSRPETHILSPPHPGSAIVKGAVALALNPGVVVARILKRTYGTSLTKRLEPGVDPRHLLKVAIDGSQWCRKRFDIFAKKGTRMEINNCVKKEYAPYKSGQSHIKFDLHSSGDEEPRYTDDPGVVSEGEFTVDLPKNPTHDNVPVYEFSLYFGRSSIELREEAKFPHIHEAERRVYRSWLEKMKKKQIQGESTYTLELTVSDYT

Target ORF information:

RefSeq Version	XM_024536196.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens heat shock 70 kDa protein 12A-like (LOC112290175), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024536196.1

ORF Insert Sequence:

ATGGCTTTCT TGAGTTGGGG CTCATCGCTG AAAAGAGAAA AATGCCCTGT GGAACGGGTA 
CGGGGTAATG AATCAAACGG AGTCGGAAAA ATAGGACCGT CGTCATCGAG AGGCTATAAC 
GATCGAGTAA TTGTCGAAGC TGCAAACCCT GGTCCGCTCC GAATCAGCAG GAATCTTCCT 
AGTGCTGTAC CAGGCACTAG CGTGGAGGAA GTGTCGGGTG CCGTTCGCAT TCCTGACGAG 
GTCAGATCTA CGCCTCAAAA TGGTTGGGTG ATAGGGTTAG ATTTCGGAAC CACATTCTCA 
GGGTTTGCCT ACGCCAAGGT TTCAGATCCT GAGCACATCT ATGTCCATCA CGACTGGCCC 
TGCATGAGCG GTGGGAACGA ATATTGCAAG ACATTGACGG GCCTTTTCTA CACAAAGACG 
GCGACTGGGG AATTGCAGTG TACGTCTTGG GGTCACCCAG CTCGATCCGA TTATATGGCG 
AATCGCGGGG ATAGTTCCAC CCGAGGATTT TACCTGTCAA AGTTCAAGCT GCTTCTGAAG 
AAAGATCTGG ACGTTTCTTC CCTGGCGAGT GCCATACCGC CGTCACTTAC AGTGAGCAAA 
ATCATCACAC AATATTTGAA GCACATCGGC GAGTATGCCT TATCCGTACT CAGGAACCAT 
GATGGAGAGG TGAGGTTCAG CCAAGACTCA GTGCAATGGT GCGTGACAGT GCCTTCAATT 
TGGGACGATG CGGCGAAGCA GCAGATGAAG GTGTGCATGA TGGACACGGG GCTTGTATCT 
TGCGATAGCG GAATAGAGTC GGTGAAAGTT GTCTTGGAAC CAGAAGCAGC TTCCTTCCAC 
TGCCACCAAC TTATGCCAAA GCAATACCCC AAAGTGTGCT TAGACAGATG TGATAATATT 
CTGGTGGCCG ACATCGGGGG TGGGACAGTC GACATCGTGG TGCAGAAGTT GAAAGGCATC 
GGGAGGGATT ACAAAGTTGA GGAGCTCACA GAAAGCTCTG GTGGTCTTTG CGGTGGGACA 
ATTGTTGACG ATTATTTCTT GCGGTTCGTG TCCAAACGAA TAGGATGCCC GGAAGAATTT 
CTGCGGTTGG ATGCCCAAAA CTGCAGGTCG CGCTTTCTCA AGGATTGGGA AGAAATCAAG 
CGTGGGTTCG GCCATGAGAT GAGGCCGACT ACGAATACAG AAATTTCACT GCACCGTAAC 
TTGGCTGAAA AGTGGGAGGA TTACGAGAGG AGACGAGGGT ATCCACCGCG AAAGTCATAT 
AGCGAAATTA AACTCACACA GCAGGATATG ATGTCCATTT TCGATCCAGT GGTGGATGAG 
ATTATCAAAC TGATTGCTGC CCAGCTAGAG AAAGTGACCA ACATCAAGGC GATATTCGTG 
GTTGGTGGAT TCGCAGGCTC TCAGTATCTA ATGCGACAAA TCCGAGCGCG TTTTTCTCGT 
CCAGAGACGC ATATCCTCAG TCCTCCTCAC CCTGGAAGCG CCATCGTTAA AGGTGCCGTT 
GCCCTGGCAC TCAATCCCGG GGTCGTTGTG GCTCGAATTC TTAAGAGAAC TTACGGCACC 
AGTCTAACCA AGCGATTAGA GCCAGGTGTT GACCCTAGGC ACTTACTGAA AGTCGCCATT 
GACGGGTCGC AGTGGTGTCG CAAGAGATTC GACATTTTCG CGAAGAAAGG GACTCGGATG 
GAGATCAACA ACTGTGTAAA GAAGGAATAC GCACCATACA AAAGCGGCCA ATCACACATC 
AAGTTCGATC TGCACAGTTC AGGTGACGAG GAACCCAGGT ACACAGATGA CCCCGGCGTT 
GTTAGCGAGG GAGAGTTCAC CGTCGACTTA CCCAAGAACC CCACACACGA CAACGTGCCG 
GTCTACGAAT TTTCTTTGTA CTTCGGGCGC AGCAGCATCG AACTTCGAGA AGAGGCCAAG 
TTCCCACACA TCCACGAGGC AGAGCGACGG GTGTATCGTA GTTGGCTCGA AAAAATGAAA 
AAGAAACAAA TCCAGGGGGA GAGCACGTAC ACCTTAGAAC TGACTGTCTC CGACTACACC 
TGA