DIAPH1 cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIAPH1
Entrez Gene ID	101106682
Full Name	diaphanous related formin 1
Gene Type	protein-coding
Organism	Ovis aries musimon(Ovis orientalis musimon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015096089.1	XP_014951575.1	LOW QUALITY PROTEIN: protein diaphanous homolog 1 isoform X1
XM_015096089.2	XP_014951575.2	LOW QUALITY PROTEIN: protein diaphanous homolog 1
XM_012127014.2	XP_011982404.1	protein diaphanous homolog 1 isoform X2
XM_012127015.2	XP_011982405.1	protein diaphanous homolog 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following DIAPH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIAPH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSm81043	XM_015096089.2 Latest version!	Ovis aries diaphanous related formin 1 (DIAPH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOv11711	XM_012127014.2 Latest version!	Ovis aries musimon diaphanous related formin 1 (DIAPH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OOv11712	XM_012127015.2 Latest version!	Ovis aries musimon diaphanous related formin 1 (DIAPH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OSm49824	XM_015096089.1 Latest version!	Ovis aries diaphanous related formin 1 (DIAPH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIAPH1 ( XM_015096089.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014951575.2 Ovis aries diaphanous related formin 1 (DIAPH1), mRNA.

Price & Availability↑

CloneID	OSm81043
Clone ID Related Accession (Same CDS sequence)	XM_015096089.2
Accession Version	XM_015096089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	LOW QUALITY PROTEIN: protein diaphanous homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_015096089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGGAGCCGC CCGGCGGGGG CCTGGGACCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAACCCGG ACGAGCTGCC TTCGGCAGGC GGCGACGGCG GCAAATCCAA GAAATTTACT 
CTGAAGAGAC TCATGGCAGA CGAGCTGGAG AGATTTACCA GCATGAGAAG TAAGAAGGAG 
AAAGAAAAGC CCAATTCTGC TCATAGAAAC TCTTCTGCAT TTTATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAG CAAGTTCTAG TACTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAACGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATTGTCATC 
AAGAGAGAGA TGGTGTCCCA GTACTTGCAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAGGT CAGCCATGAT GTACATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTC TCCCTCAACA ACAACCCTGT CAGTTGGGTC 
CAAACATTTG GTGCTGAGGG CCTGGCCTCC TTATTGGACA TCCTTAAACG ACTTCATGAT 
GAGAAGGAGG AGACTGCTGG AGGCTACGAC AGCCGTAACA AGCATGAAAT CATTCGCTGC 
TTGAAAGCTT TTATGAATAA CAAGTTTGGA ATCAAGACCA TGCTGGAGAC AGAAGAAGGA 
ATTCTGCTGC TGGTCAGAGC CATGGATCCT GCGGTTCCCA GCATGATGAT TGACGCTGCT 
AAGCTGTTGT CTGCCCTCTG CATCCTGCCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCGATGA CAGAAAGAGC TGAAATGGAT GAGGTGGAAC GTTTTCAGCC ACTGCTGGAT 
GGATTAAAAA GTGGGACCTC CATTGCTCTC AAGGTGGGAT GCCTGCAGCT CATCAATGCT 
CTCATCACAC CAGCTGAAGA ACTTGACTTC CGAGTTCACA TCCGAAGTGA ACTGATGCGC 
TTGGGGCTGC ACCAAGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAGCTTAATG TATTTGATGA ACAAGGAGAA GAAGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GAATGGAGAT GGATGACTTC AGTGAAATCT TCCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCAGCACTTC CTGTCCATCC TGCAGCACTT ACTCTTAGTC 
CGAAATGACT ATGAGGCCAG ACCGCAGTAC TATAAGTTGA TTGAAGAATG TATTTCTCAG 
ATAGTTCTGC ATAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAC 
ATTGAGGGAC TGATTGACCA AATGATTGAT AAAACAAAGG TGGAGAAATC AGAAGCCAAA 
GCTACAGAGC TGGAAAAAAA GCTGGACTCT GAGTTAACAG CTCGGCATGA GCTCCAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTCC AGGATATTCA GGGAGAAAAG 
GATTCATTGG ATTCTGAAAA GCAAAAAATT GCCACAGAGA AGCAGGACCT GGAAGCAGAA 
GTGTCTCAGC TCACAGGAGA GGTCGCCAAG CTGTCCAAGG AACTGGAAGA TGCCAAGAAA 
GAAGTGGCTT CTCTCTCTGC TGCAGTTACT GCTGTAGCCC CTCCTAGCAG TGCTACTGTT 
ACCCCTGCCC CTTCTTTACC TGGCGACTCT GGGCCCGTTA TACCCCCCTT ATCAGTCCCC 
ACACCCCCTC TTCCTGAGGC CAGTGTCAGT CTTCCTCCAC CACCTCCTGC TCCTCCTCTA 
CCTGGGTGTG CTTTCATCTC CCCACCTGGG GGTCCTAGCA TCCCCCCTCC TCCCCCTTTG 
CCTGGAGGTG GTGGCATCCT CCCACCTCCT CCTGCCCCTT TGCCTGGAGG TCCTGGCATC 
CCCCCACCTC CTCCCCTTCC TGGAGGTCCT GGCATCCCCC CACCCCCTCC TCCCCTGCCT 
GGAGGTCCTG GCCTCCCCCC ACCCCCTCCT CCCCTGCCTG GAGGTCCTGG CCTCCCCCCA 
CCCCCCCTGC CTCCCCCTCC CCTGCCTGGA GGTCCTGGCA TCCCCCCACC CCCTCCTCCC 
CTGCCTGGAG GTCCTGGCAT CCCACCCCCT CCTCCCCTGC CTGGAGGTCC TGGTCTTCCC 
CCACCCCCTC CTCCCTTGCC TGGAGGACCA GGAATGCTAC CTCCCCCTCC CCCTCTTCCT 
GGTGGAATCC CTCCACCCCC TCCATTTCCT GGAGGCCCTG GCATTCCTCC ACCTCTCCCT 
TTTGGAGTTC CTGCAGCCCC AGTTCTGCCA TTTGGATTAA CCCCAAAGAA ACTTTACAAG 
CCAGAGGTGC AGCTCCGGAG GCCAAACTGG TCCAAGTTTG TCGCTGAGGA CCTTTCCCAG 
GACTGCTTCT GGACAAAGGT GAAGGAGGAC CGATTTGAGA ACAGTGAACT TTTCGCCAAA 
CTTACCAGTA CCTTTTCTGC CCAGACCAAG ACTTCAAAAG CCAAGAAGGA TCAGGAAGGT 
GGAGAAGAAA AGAAATCTCT GCAAAAGAAA AAAGTGAAAG AGTTAAAGGT GTTGGATTCC 
AAGACAGCCC AGAATCTCTC AATCTTTTTG GGTTCCTTCC GCATGCCCTA TCATGAGATC 
AAGAATGTCA TCCTGGAGGT GAATGAGTCT GTTCTCACTG AGTCCATGAT CCAGAACCTC 
ATTAAGCAGA TGCCAGAGCC AGAGCAGTTA AAAATGCTTT CTGAACTGAA GGACGAATAT 
GATGATTTGG CTGAGTCAGA GCAGTTTGGT GTGGTGATGG GTGCAGTACC CCGCCTGCGG 
CCTCGCCTCA ATGCCATCCT CTTCAAGCTG CAGTTCGGGG AGCAGGTGGA GAACATCAAG 
CCCGAGATCG TCTCGGTGAC TGCCGCATGT GAGGAGGTGC GAAAGAGCGA GAACTTCTCT 
AACCTCCTGG AGATAACCTT GCTTGTCGGA AACTATATGA ATGCTGGCTC CAGGAATGCT 
GGTGCTTTTG GCTTCAACAT CAGCTTCCTT TGTAAGCTTC GAGACACCAA GTCCACAGAT 
CAGAAGATGA CGTTGTTGCA CTTCTTGGCT GACTTGTGTG AGCATGATCA TCCCGAGGTC 
CTGAAGTTCC CGGACGAGCT TGCCCACGTG GAGAAAGCCA GCCGAGTTTC TGCAGAAAAC 
TTGCAAAAGA ACCTAGATCA GATGAAGAAA CAGATTTCCG ATGTGGAACG TGATATTCAG 
AATTTTCCAG CTGCCACAGA AGAGAAAGAC AAGTTTGTTG AAAAAATGAC CAGCTTTGTG 
AAGGATGCAC AGGAACAGTA CAACAAGCTG CGGATGATGC ACTCTAACAT GGAGATGCTC 
TACAAGGAGC TGGGCGAGTA CTTCCTCTTT GACCCCAAGA AGGTGTCTGT GGAAGAATTC 
TTCATGGATC TGCACAACTT TAAGAATATG TTTGTGCAAG CCGTCAAGGA GAACCAGAAG 
CGGCGGGAGA CAGAGGAGAA GATGCGGCGA GCAAAGCTGG CCAAGGAGAA GGCAGAGAAG 
GAGCGGTTGG AGAAGCAGCA GAAGAGAGAG CAACTCATAG ACATGAATGC TGAGGGTGAT 
GAGACGGGTG TGATGGACAG TCTTCTGGAA GCCCTGCAGT CAGGCGCAGC ATTCCGACGG 
AAGAGAGGAC CCCGTCAGGG CGCCAGGAAG GCAGCGTGTG CAGCCACATC TCAGCTAGTC 
TCAGAGCTGA CCAAGGAGGA TGCCATGACC TGTGTTCCTG TCAAGATGCC TAAGAAGGAG 
GAAGTCCCCA CCATCCTCGA GGAGACCACA GAGTTGCTTG GCCGTGCAAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014951575.2
CDS	72..3965
Translation	MEPPGGGLGPGRGTRDKKKGRNPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRSKKEKEKPNSAHRNSSAFYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMSQKESSRSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGGYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPSMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFSEIFQILLNTVKDSKAEQHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIDIEGLIDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMESDFEQKLQDIQGEKDSLDSEKQKIATEKQDLEAEVSQLTGEVAKLSKELEDAKKEVASLSAAVTAVAPPSSATVTPAPSLPGDSGPVIPPLSVPTPPLPEASVSLPPPPPAPPLPGCAFISPPGGPSIPPPPPLPGGGGILPPPPAPLPGGPGIPPPPPLPGGPGIPPPPPPLPGGPGLPPPPPPLPGGPGLPPPPLPPPPLPGGPGIPPPPPPLPGGPGIPPPPPLPGGPGLPPPPPPLPGGPGMLPPPPPLPGGIPPPPPFPGGPGIPPPLPFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENSELFAKLTSTFSAQTKTSKAKKDQEGGEEKKSLQKKKVKELKVLDSKTAQNLSIFLGSFRMPYHEIKNVILEVNESVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGAVPRLRPRLNAILFKLQFGEQVENIKPEIVSVTAACEEVRKSENFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLADLCEHDHPEVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDIQNFPAATEEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMEMLYKELGEYFLFDPKKVSVEEFFMDLHNFKNMFVQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQGARKAACAATSQLVSELTKEDAMTCVPVKMPKKEEVPTILEETTELLGRAS

Target ORF information:

RefSeq Version	XM_015096089.2
Organism	Ovis aries(sheep)
Definition	Ovis aries diaphanous related formin 1 (DIAPH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015096089.2

ORF Insert Sequence:

ATGGAGCCGC CCGGCGGGGG CCTGGGACCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAACCCGG ACGAGCTGCC TTCGGCAGGC GGCGACGGCG GCAAATCCAA GAAATTTACT 
CTGAAGAGAC TCATGGCAGA CGAGCTGGAG AGATTTACCA GCATGAGAAG TAAGAAGGAG 
AAAGAAAAGC CCAATTCTGC TCATAGAAAC TCTTCTGCAT TTTATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAG CAAGTTCTAG TACTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAACGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATTGTCATC 
AAGAGAGAGA TGGTGTCCCA GTACTTGCAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAGGT CAGCCATGAT GTACATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTC TCCCTCAACA ACAACCCTGT CAGTTGGGTC 
CAAACATTTG GTGCTGAGGG CCTGGCCTCC TTATTGGACA TCCTTAAACG ACTTCATGAT 
GAGAAGGAGG AGACTGCTGG AGGCTACGAC AGCCGTAACA AGCATGAAAT CATTCGCTGC 
TTGAAAGCTT TTATGAATAA CAAGTTTGGA ATCAAGACCA TGCTGGAGAC AGAAGAAGGA 
ATTCTGCTGC TGGTCAGAGC CATGGATCCT GCGGTTCCCA GCATGATGAT TGACGCTGCT 
AAGCTGTTGT CTGCCCTCTG CATCCTGCCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCGATGA CAGAAAGAGC TGAAATGGAT GAGGTGGAAC GTTTTCAGCC ACTGCTGGAT 
GGATTAAAAA GTGGGACCTC CATTGCTCTC AAGGTGGGAT GCCTGCAGCT CATCAATGCT 
CTCATCACAC CAGCTGAAGA ACTTGACTTC CGAGTTCACA TCCGAAGTGA ACTGATGCGC 
TTGGGGCTGC ACCAAGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAGCTTAATG TATTTGATGA ACAAGGAGAA GAAGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GAATGGAGAT GGATGACTTC AGTGAAATCT TCCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCAGCACTTC CTGTCCATCC TGCAGCACTT ACTCTTAGTC 
CGAAATGACT ATGAGGCCAG ACCGCAGTAC TATAAGTTGA TTGAAGAATG TATTTCTCAG 
ATAGTTCTGC ATAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAC 
ATTGAGGGAC TGATTGACCA AATGATTGAT AAAACAAAGG TGGAGAAATC AGAAGCCAAA 
GCTACAGAGC TGGAAAAAAA GCTGGACTCT GAGTTAACAG CTCGGCATGA GCTCCAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTCC AGGATATTCA GGGAGAAAAG 
GATTCATTGG ATTCTGAAAA GCAAAAAATT GCCACAGAGA AGCAGGACCT GGAAGCAGAA 
GTGTCTCAGC TCACAGGAGA GGTCGCCAAG CTGTCCAAGG AACTGGAAGA TGCCAAGAAA 
GAAGTGGCTT CTCTCTCTGC TGCAGTTACT GCTGTAGCCC CTCCTAGCAG TGCTACTGTT 
ACCCCTGCCC CTTCTTTACC TGGCGACTCT GGGCCCGTTA TACCCCCCTT ATCAGTCCCC 
ACACCCCCTC TTCCTGAGGC CAGTGTCAGT CTTCCTCCAC CACCTCCTGC TCCTCCTCTA 
CCTGGGTGTG CTTTCATCTC CCCACCTGGG GGTCCTAGCA TCCCCCCTCC TCCCCCTTTG 
CCTGGAGGTG GTGGCATCCT CCCACCTCCT CCTGCCCCTT TGCCTGGAGG TCCTGGCATC 
CCCCCACCTC CTCCCCTTCC TGGAGGTCCT GGCATCCCCC CACCCCCTCC TCCCCTGCCT 
GGAGGTCCTG GCCTCCCCCC ACCCCCTCCT CCCCTGCCTG GAGGTCCTGG CCTCCCCCCA 
CCCCCCCTGC CTCCCCCTCC CCTGCCTGGA GGTCCTGGCA TCCCCCCACC CCCTCCTCCC 
CTGCCTGGAG GTCCTGGCAT CCCACCCCCT CCTCCCCTGC CTGGAGGTCC TGGTCTTCCC 
CCACCCCCTC CTCCCTTGCC TGGAGGACCA GGAATGCTAC CTCCCCCTCC CCCTCTTCCT 
GGTGGAATCC CTCCACCCCC TCCATTTCCT GGAGGCCCTG GCATTCCTCC ACCTCTCCCT 
TTTGGAGTTC CTGCAGCCCC AGTTCTGCCA TTTGGATTAA CCCCAAAGAA ACTTTACAAG 
CCAGAGGTGC AGCTCCGGAG GCCAAACTGG TCCAAGTTTG TCGCTGAGGA CCTTTCCCAG 
GACTGCTTCT GGACAAAGGT GAAGGAGGAC CGATTTGAGA ACAGTGAACT TTTCGCCAAA 
CTTACCAGTA CCTTTTCTGC CCAGACCAAG ACTTCAAAAG CCAAGAAGGA TCAGGAAGGT 
GGAGAAGAAA AGAAATCTCT GCAAAAGAAA AAAGTGAAAG AGTTAAAGGT GTTGGATTCC 
AAGACAGCCC AGAATCTCTC AATCTTTTTG GGTTCCTTCC GCATGCCCTA TCATGAGATC 
AAGAATGTCA TCCTGGAGGT GAATGAGTCT GTTCTCACTG AGTCCATGAT CCAGAACCTC 
ATTAAGCAGA TGCCAGAGCC AGAGCAGTTA AAAATGCTTT CTGAACTGAA GGACGAATAT 
GATGATTTGG CTGAGTCAGA GCAGTTTGGT GTGGTGATGG GTGCAGTACC CCGCCTGCGG 
CCTCGCCTCA ATGCCATCCT CTTCAAGCTG CAGTTCGGGG AGCAGGTGGA GAACATCAAG 
CCCGAGATCG TCTCGGTGAC TGCCGCATGT GAGGAGGTGC GAAAGAGCGA GAACTTCTCT 
AACCTCCTGG AGATAACCTT GCTTGTCGGA AACTATATGA ATGCTGGCTC CAGGAATGCT 
GGTGCTTTTG GCTTCAACAT CAGCTTCCTT TGTAAGCTTC GAGACACCAA GTCCACAGAT 
CAGAAGATGA CGTTGTTGCA CTTCTTGGCT GACTTGTGTG AGCATGATCA TCCCGAGGTC 
CTGAAGTTCC CGGACGAGCT TGCCCACGTG GAGAAAGCCA GCCGAGTTTC TGCAGAAAAC 
TTGCAAAAGA ACCTAGATCA GATGAAGAAA CAGATTTCCG ATGTGGAACG TGATATTCAG 
AATTTTCCAG CTGCCACAGA AGAGAAAGAC AAGTTTGTTG AAAAAATGAC CAGCTTTGTG 
AAGGATGCAC AGGAACAGTA CAACAAGCTG CGGATGATGC ACTCTAACAT GGAGATGCTC 
TACAAGGAGC TGGGCGAGTA CTTCCTCTTT GACCCCAAGA AGGTGTCTGT GGAAGAATTC 
TTCATGGATC TGCACAACTT TAAGAATATG TTTGTGCAAG CCGTCAAGGA GAACCAGAAG 
CGGCGGGAGA CAGAGGAGAA GATGCGGCGA GCAAAGCTGG CCAAGGAGAA GGCAGAGAAG 
GAGCGGTTGG AGAAGCAGCA GAAGAGAGAG CAACTCATAG ACATGAATGC TGAGGGTGAT 
GAGACGGGTG TGATGGACAG TCTTCTGGAA GCCCTGCAGT CAGGCGCAGC ATTCCGACGG 
AAGAGAGGAC CCCGTCAGGG CGCCAGGAAG GCAGCGTGTG CAGCCACATC TCAGCTAGTC 
TCAGAGCTGA CCAAGGAGGA TGCCATGACC TGTGTTCCTG TCAAGATGCC TAAGAAGGAG 
GAAGTCCCCA CCATCCTCGA GGAGACCACA GAGTTGCTTG GCCGTGCAAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( XM_012127014.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011982404.1 Ovis aries musimon diaphanous related formin 1 (DIAPH1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv11711
Clone ID Related Accession (Same CDS sequence)	XM_012127014.2
Accession Version	XM_012127014.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	protein diaphanous homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012127014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC CCGGCGGGGG CCTGGGACCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAACCCGG ACGAGCTGCC TTCGGCAGGC GGCGACGGCG GCAAATCCAA GAAATTTACT 
CTGAAGAGAC TCATGGCAGA CGAGCTGGAG AGATTTACCA GCATGAGAAG TAAGAAGGAG 
AAAGAAAAGC CCAATTCTGC TCATAGAAAC TCTTCTGCAT TTTATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAG CAAGTTCTAG TACTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAACGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATTGTCATC 
AAGAGAGAGA TGGTGTCCCA GTACTTGCAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAGGT CAGCCATGAT GTACATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTC TCCCTCAACA ACAACCCTGT CAGTTGGGTC 
CAAACATTTG GTGCTGAGGG CCTGGCCTCC TTATTGGACA TCCTTAAACG ACTTCATGAT 
GAGAAGGAGG AGACTGCTGG AGGCTACGAC AGCCGTAACA AGCATGAAAT CATTCGCTGC 
TTGAAAGCTT TTATGAATAA CAAGTTTGGA ATCAAGACCA TGCTGGAGAC AGAAGAAGGA 
ATTCTGCTGC TGGTCAGAGC CATGGATCCT GCGGTTCCCA GCATGATGAT TGACGCTGCT 
AAGCTGTTGT CTGCCCTCTG CATCCTGCCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCGATGA CAGAAAGAGC TGAAATGGAT GAGGTGGAAC GTTTTCAGCC ACTGCTGGAT 
GGATTAAAAA GTGGGACCTC CATTGCTCTC AAGGTGGGAT GCCTGCAGCT CATCAATGCT 
CTCATCACAC CAGCTGAAGA ACTTGACTTC CGAGTTCACA TCCGAAGTGA ACTGATGCGC 
TTGGGGCTGC ACCAAGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAGCTTAATG TATTTGATGA ACAAGGAGAA GAAGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GAATGGAGAT GGATGACTTC AGTGAAATCT TCCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCAGCACTTC CTGTCCATCC TGCAGCACTT ACTCTTAGTC 
CGAAATGACT ATGAGGCCAG ACCGCAGTAC TATAAGTTGA TTGAAGAATG TATTTCTCAG 
ATAGTTCTGC ATAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAC 
ATTGAGGGAC TGATTGACCA AATGATTGAT AAAACAAAGG TGGAGAAATC AGAAGCCAAA 
GCTACAGAGC TGGAAAAAAA GCTGGACTCT GAGTTAACAG CTCGGCATGA GCTCCAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTCC AGGATATTCA GGGAGAAAAG 
GATTCATTGG ATTCTGAAAA GCAAAAAATT GCCACAGAGA AGCAGGACCT GGAAGCAGAA 
GTGTCTCAGC TCACAGGAGA GGTCGCCAAG CTGTCCAAGG AACTGGAAGA TGCCAAGAAA 
GAAGTGGCTT CTCTCTCTGC TGCAGTTACT GCTGTAGCCC CTCCTAGCAG TGCTACTGTT 
ACCCCTGCCC CTTCTTTACC TGGCGACTCT GGGCCCGTTA TACCCCCCTT ATCAGTCCCC 
ACACCCCCTC TTCCTGAGGC CAGTGTCAGT CTTCCTCCAC CACCTCCTGC TCCTCCTCTA 
CCTGGGTGTG CTTTCATCTC CCCACCTGGG GGTCCTAGCA TCCCCCCTCC TCCCCCTTTG 
CCTGGAGGTG GTGGCATCCT CCCACCTCCT CCTGCCCCTT TGCCTGGAGG TCCTGGCATC 
CCCCCACCTC CTCCCCTTCC TGGAGGTCCT GGCATCCCCC CACCCCCTCC TCCCCTGCCT 
GGAGGTCCTG GCCTCCCCCC ACCCCCTCCT CCCCTGCCTG GAGGTCCTGG CATCCCCCCA 
CCCCCTCCTC CCCTGCCTGG AGGTCCTGGT CTTCCCCCAC CCCCTCCTCC CTTGCCTGGA 
GGACCAGGAA TGCTACCTCC CCCTCCCCCT CTTCCTGGTG GAATCCCTCC ACCCCCTCCA 
TTTCCTGGAG GCCCTGGCAT TCCTCCACCT CTCCCTTTTG GAGTTCCTGC AGCCCCAGTT 
CTGCCATTTG GATTAACCCC AAAGAAACTT TACAAGCCAG AGGTGCAGCT CCGGAGGCCA 
AACTGGTCCA AGTTTGTCGC TGAGGACCTT TCCCAGGACT GCTTCTGGAC AAAGGTGAAG 
GAGGACCGAT TTGAGAACAG TGAACTTTTC GCCAAACTTA CCAGTACCTT TTCTGCCCAG 
ACCAAGACTT CAAAAGCCAA GAAGGATCAG GAAGGTGGAG AAGAAAAGAA ATCTCTGCAA 
AAGAAAAAAG TGAAAGAGTT AAAGGTGTTG GATTCCAAGA CAGCCCAGAA TCTCTCAATC 
TTTTTGGGTT CCTTCCGCAT GCCCTATCAT GAGATCAAGA ATGTCATCCT GGAGGTGAAT 
GAGTCTGTTC TCACTGAGTC CATGATCCAG AACCTCATTA AGCAGATGCC AGAGCCAGAG 
CAGTTAAAAA TGCTTTCTGA ACTGAAGGAC GAATATGATG ATTTGGCTGA GTCAGAGCAG 
TTTGGTGTGG TGATGGGTGC AGTACCCCGC CTGCGGCCTC GCCTCAATGC CATCCTCTTC 
AAGCTGCAGT TCGGGGAGCA GGTGGAGAAC ATCAAGCCCG AGATCGTCTC GGTGACTGCC 
GCATGTGAGG AGGTGCGAAA GAGCGAGAAC TTCTCTAACC TCCTGGAGAT AACCTTGCTT 
GTCGGAAACT ATATGAATGC TGGCTCCAGG AATGCTGGTG CTTTTGGCTT CAACATCAGC 
TTCCTTTGTA AGCTTCGAGA CACCAAGTCC ACAGATCAGA AGATGACGTT GTTGCACTTC 
TTGGCTGACT TGTGTGAGCA TGATCATCCC GAGGTCCTGA AGTTCCCGGA CGAGCTTGCC 
CACGTGGAGA AAGCCAGCCG AGTTTCTGCA GAAAACTTGC AAAAGAACCT AGATCAGATG 
AAGAAACAGA TTTCCGATGT GGAACGTGAT ATTCAGAATT TTCCAGCTGC CACAGAAGAG 
AAAGACAAGT TTGTTGAAAA AATGACCAGC TTTGTGAAGG ATGCACAGGA ACAGTACAAC 
AAGCTGCGGA TGATGCACTC TAACATGGAG ATGCTCTACA AGGAGCTGGG CGAGTACTTC 
CTCTTTGACC CCAAGAAGGT GTCTGTGGAA GAATTCTTCA TGGATCTGCA CAACTTTAAG 
AATATGTTTG TGCAAGCCGT CAAGGAGAAC CAGAAGCGGC GGGAGACAGA GGAGAAGATG 
CGGCGAGCAA AGCTGGCCAA GGAGAAGGCA GAGAAGGAGC GGTTGGAGAA GCAGCAGAAG 
AGAGAGCAAC TCATAGACAT GAATGCTGAG GGTGATGAGA CGGGTGTGAT GGACAGTCTT 
CTGGAAGCCC TGCAGTCAGG CGCAGCATTC CGACGGAAGA GAGGACCCCG TCAGGGCGCC 
AGGAAGGCAG CGTGTGCAGC CACATCTCAG CTAGTCTCAG AGCTGACCAA GGAGGATGCC 
ATGACCTGTG TTCCTGTCAA GATGCCTAAG AAGGAGGAAG TCCCCACCAT CCTCGAGGAG 
ACCACAGAGT TGCTTGGCCG TGCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011982404.1
CDS	27..3836
Translation	MEPPGGGLGPGRGTRDKKKGRNPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRSKKEKEKPNSAHRNSSAFYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMSQKESSRSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGGYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPSMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFSEIFQILLNTVKDSKAEQHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIDIEGLIDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMESDFEQKLQDIQGEKDSLDSEKQKIATEKQDLEAEVSQLTGEVAKLSKELEDAKKEVASLSAAVTAVAPPSSATVTPAPSLPGDSGPVIPPLSVPTPPLPEASVSLPPPPPAPPLPGCAFISPPGGPSIPPPPPLPGGGGILPPPPAPLPGGPGIPPPPPLPGGPGIPPPPPPLPGGPGLPPPPPPLPGGPGIPPPPPPLPGGPGLPPPPPPLPGGPGMLPPPPPLPGGIPPPPPFPGGPGIPPPLPFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENSELFAKLTSTFSAQTKTSKAKKDQEGGEEKKSLQKKKVKELKVLDSKTAQNLSIFLGSFRMPYHEIKNVILEVNESVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGAVPRLRPRLNAILFKLQFGEQVENIKPEIVSVTAACEEVRKSENFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLADLCEHDHPEVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDIQNFPAATEEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMEMLYKELGEYFLFDPKKVSVEEFFMDLHNFKNMFVQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQGARKAACAATSQLVSELTKEDAMTCVPVKMPKKEEVPTILEETTELLGRAS

Target ORF information:

RefSeq Version	XM_012127014.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon diaphanous related formin 1 (DIAPH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012127014.2

ORF Insert Sequence:

ATGGAGCCGC CCGGCGGGGG CCTGGGACCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAACCCGG ACGAGCTGCC TTCGGCAGGC GGCGACGGCG GCAAATCCAA GAAATTTACT 
CTGAAGAGAC TCATGGCAGA CGAGCTGGAG AGATTTACCA GCATGAGAAG TAAGAAGGAG 
AAAGAAAAGC CCAATTCTGC TCATAGAAAC TCTTCTGCAT TTTATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAG CAAGTTCTAG TACTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAACGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATTGTCATC 
AAGAGAGAGA TGGTGTCCCA GTACTTGCAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAGGT CAGCCATGAT GTACATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTC TCCCTCAACA ACAACCCTGT CAGTTGGGTC 
CAAACATTTG GTGCTGAGGG CCTGGCCTCC TTATTGGACA TCCTTAAACG ACTTCATGAT 
GAGAAGGAGG AGACTGCTGG AGGCTACGAC AGCCGTAACA AGCATGAAAT CATTCGCTGC 
TTGAAAGCTT TTATGAATAA CAAGTTTGGA ATCAAGACCA TGCTGGAGAC AGAAGAAGGA 
ATTCTGCTGC TGGTCAGAGC CATGGATCCT GCGGTTCCCA GCATGATGAT TGACGCTGCT 
AAGCTGTTGT CTGCCCTCTG CATCCTGCCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCGATGA CAGAAAGAGC TGAAATGGAT GAGGTGGAAC GTTTTCAGCC ACTGCTGGAT 
GGATTAAAAA GTGGGACCTC CATTGCTCTC AAGGTGGGAT GCCTGCAGCT CATCAATGCT 
CTCATCACAC CAGCTGAAGA ACTTGACTTC CGAGTTCACA TCCGAAGTGA ACTGATGCGC 
TTGGGGCTGC ACCAAGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAGCTTAATG TATTTGATGA ACAAGGAGAA GAAGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GAATGGAGAT GGATGACTTC AGTGAAATCT TCCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCAGCACTTC CTGTCCATCC TGCAGCACTT ACTCTTAGTC 
CGAAATGACT ATGAGGCCAG ACCGCAGTAC TATAAGTTGA TTGAAGAATG TATTTCTCAG 
ATAGTTCTGC ATAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAC 
ATTGAGGGAC TGATTGACCA AATGATTGAT AAAACAAAGG TGGAGAAATC AGAAGCCAAA 
GCTACAGAGC TGGAAAAAAA GCTGGACTCT GAGTTAACAG CTCGGCATGA GCTCCAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTCC AGGATATTCA GGGAGAAAAG 
GATTCATTGG ATTCTGAAAA GCAAAAAATT GCCACAGAGA AGCAGGACCT GGAAGCAGAA 
GTGTCTCAGC TCACAGGAGA GGTCGCCAAG CTGTCCAAGG AACTGGAAGA TGCCAAGAAA 
GAAGTGGCTT CTCTCTCTGC TGCAGTTACT GCTGTAGCCC CTCCTAGCAG TGCTACTGTT 
ACCCCTGCCC CTTCTTTACC TGGCGACTCT GGGCCCGTTA TACCCCCCTT ATCAGTCCCC 
ACACCCCCTC TTCCTGAGGC CAGTGTCAGT CTTCCTCCAC CACCTCCTGC TCCTCCTCTA 
CCTGGGTGTG CTTTCATCTC CCCACCTGGG GGTCCTAGCA TCCCCCCTCC TCCCCCTTTG 
CCTGGAGGTG GTGGCATCCT CCCACCTCCT CCTGCCCCTT TGCCTGGAGG TCCTGGCATC 
CCCCCACCTC CTCCCCTTCC TGGAGGTCCT GGCATCCCCC CACCCCCTCC TCCCCTGCCT 
GGAGGTCCTG GCCTCCCCCC ACCCCCTCCT CCCCTGCCTG GAGGTCCTGG CATCCCCCCA 
CCCCCTCCTC CCCTGCCTGG AGGTCCTGGT CTTCCCCCAC CCCCTCCTCC CTTGCCTGGA 
GGACCAGGAA TGCTACCTCC CCCTCCCCCT CTTCCTGGTG GAATCCCTCC ACCCCCTCCA 
TTTCCTGGAG GCCCTGGCAT TCCTCCACCT CTCCCTTTTG GAGTTCCTGC AGCCCCAGTT 
CTGCCATTTG GATTAACCCC AAAGAAACTT TACAAGCCAG AGGTGCAGCT CCGGAGGCCA 
AACTGGTCCA AGTTTGTCGC TGAGGACCTT TCCCAGGACT GCTTCTGGAC AAAGGTGAAG 
GAGGACCGAT TTGAGAACAG TGAACTTTTC GCCAAACTTA CCAGTACCTT TTCTGCCCAG 
ACCAAGACTT CAAAAGCCAA GAAGGATCAG GAAGGTGGAG AAGAAAAGAA ATCTCTGCAA 
AAGAAAAAAG TGAAAGAGTT AAAGGTGTTG GATTCCAAGA CAGCCCAGAA TCTCTCAATC 
TTTTTGGGTT CCTTCCGCAT GCCCTATCAT GAGATCAAGA ATGTCATCCT GGAGGTGAAT 
GAGTCTGTTC TCACTGAGTC CATGATCCAG AACCTCATTA AGCAGATGCC AGAGCCAGAG 
CAGTTAAAAA TGCTTTCTGA ACTGAAGGAC GAATATGATG ATTTGGCTGA GTCAGAGCAG 
TTTGGTGTGG TGATGGGTGC AGTACCCCGC CTGCGGCCTC GCCTCAATGC CATCCTCTTC 
AAGCTGCAGT TCGGGGAGCA GGTGGAGAAC ATCAAGCCCG AGATCGTCTC GGTGACTGCC 
GCATGTGAGG AGGTGCGAAA GAGCGAGAAC TTCTCTAACC TCCTGGAGAT AACCTTGCTT 
GTCGGAAACT ATATGAATGC TGGCTCCAGG AATGCTGGTG CTTTTGGCTT CAACATCAGC 
TTCCTTTGTA AGCTTCGAGA CACCAAGTCC ACAGATCAGA AGATGACGTT GTTGCACTTC 
TTGGCTGACT TGTGTGAGCA TGATCATCCC GAGGTCCTGA AGTTCCCGGA CGAGCTTGCC 
CACGTGGAGA AAGCCAGCCG AGTTTCTGCA GAAAACTTGC AAAAGAACCT AGATCAGATG 
AAGAAACAGA TTTCCGATGT GGAACGTGAT ATTCAGAATT TTCCAGCTGC CACAGAAGAG 
AAAGACAAGT TTGTTGAAAA AATGACCAGC TTTGTGAAGG ATGCACAGGA ACAGTACAAC 
AAGCTGCGGA TGATGCACTC TAACATGGAG ATGCTCTACA AGGAGCTGGG CGAGTACTTC 
CTCTTTGACC CCAAGAAGGT GTCTGTGGAA GAATTCTTCA TGGATCTGCA CAACTTTAAG 
AATATGTTTG TGCAAGCCGT CAAGGAGAAC CAGAAGCGGC GGGAGACAGA GGAGAAGATG 
CGGCGAGCAA AGCTGGCCAA GGAGAAGGCA GAGAAGGAGC GGTTGGAGAA GCAGCAGAAG 
AGAGAGCAAC TCATAGACAT GAATGCTGAG GGTGATGAGA CGGGTGTGAT GGACAGTCTT 
CTGGAAGCCC TGCAGTCAGG CGCAGCATTC CGACGGAAGA GAGGACCCCG TCAGGGCGCC 
AGGAAGGCAG CGTGTGCAGC CACATCTCAG CTAGTCTCAG AGCTGACCAA GGAGGATGCC 
ATGACCTGTG TTCCTGTCAA GATGCCTAAG AAGGAGGAAG TCCCCACCAT CCTCGAGGAG 
ACCACAGAGT TGCTTGGCCG TGCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( XM_012127015.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_011982405.1 Ovis aries musimon diaphanous related formin 1 (DIAPH1), transcript variant X3, mRNA.

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CloneID	OOv11712
Clone ID Related Accession (Same CDS sequence)	XM_012127015.2
Accession Version	XM_012127015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	protein diaphanous homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012127015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC CCGGCGGGGG CCTGGGACCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAACCCGG ACGAGCTGCC TTCGGCAGGC GGCGACGGCG GCAAATCCAA GAAATTTCTG 
GAGAGATTTA CCAGCATGAG AAGTAAGAAG GAGAAAGAAA AGCCCAATTC TGCTCATAGA 
AACTCTTCTG CATTTTATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT 
GAGCAAGTTC TAGTACTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA CGAGGAGAAA 
CAGCAACCTT TGAGGGAGAA GGACATTGTC ATCAAGAGAG AGATGGTGTC CCAGTACTTG 
CACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA GGTCAGCCAT GATGTACATT 
CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT 
GTCTCCCTCA ACAACAACCC TGTCAGTTGG GTCCAAACAT TTGGTGCTGA GGGCCTGGCC 
TCCTTATTGG ACATCCTTAA ACGACTTCAT GATGAGAAGG AGGAGACTGC TGGAGGCTAC 
GACAGCCGTA ACAAGCATGA AATCATTCGC TGCTTGAAAG CTTTTATGAA TAACAAGTTT 
GGAATCAAGA CCATGCTGGA GACAGAAGAA GGAATTCTGC TGCTGGTCAG AGCCATGGAT 
CCTGCGGTTC CCAGCATGAT GATTGACGCT GCTAAGCTGT TGTCTGCCCT CTGCATCCTG 
CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCGA TGACAGAAAG AGCTGAAATG 
GATGAGGTGG AACGTTTTCA GCCACTGCTG GATGGATTAA AAAGTGGGAC CTCCATTGCT 
CTCAAGGTGG GATGCCTGCA GCTCATCAAT GCTCTCATCA CACCAGCTGA AGAACTTGAC 
TTCCGAGTTC ACATCCGAAG TGAACTGATG CGCTTGGGGC TGCACCAAGT GTTGCAGGAC 
CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAGCTTA ATGTATTTGA TGAACAAGGA 
GAAGAAGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGAATGGA GATGGATGAC 
TTCAGTGAAA TCTTCCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCAGCAC 
TTCCTGTCCA TCCTGCAGCA CTTACTCTTA GTCCGAAATG ACTATGAGGC CAGACCGCAG 
TACTATAAGT TGATTGAAGA ATGTATTTCT CAGATAGTTC TGCATAAGAA CGGGGCTGAT 
CCTGACTTCA AGTGCCGGCA CCTCCAGATT GACATTGAGG GACTGATTGA CCAAATGATT 
GATAAAACAA AGGTGGAGAA ATCAGAAGCC AAAGCTACAG AGCTGGAAAA AAAGCTGGAC 
TCTGAGTTAA CAGCTCGGCA TGAGCTCCAG GTGGAAATGA AAAAGATGGA AAGTGACTTT 
GAGCAGAAGC TCCAGGATAT TCAGGGAGAA AAGGATTCAT TGGATTCTGA AAAGCAAAAA 
ATTGCCACAG AGAAGCAGGA CCTGGAAGCA GAAGTGTCTC AGCTCACAGG AGAGGTCGCC 
AAGCTGTCCA AGGAACTGGA AGATGCCAAG AAAGAAGTGG CTTCTCTCTC TGCTGCAGTT 
ACTGCTGTAG CCCCTCCTAG CAGTGCTACT GTTACCCCTG CCCCTTCTTT ACCTGGCGAC 
TCTGGGCCCG TTATACCCCC CTTATCAGTC CCCACACCCC CTCTTCCTGA GGCCAGTGTC 
AGTCTTCCTC CACCACCTCC TGCTCCTCCT CTACCTGGGT GTGCTTTCAT CTCCCCACCT 
GGGGGTCCTA GCATCCCCCC TCCTCCCCCT TTGCCTGGAG GTGGTGGCAT CCTCCCACCT 
CCTCCTGCCC CTTTGCCTGG AGGTCCTGGC ATCCCCCCAC CTCCTCCCCT TCCTGGAGGT 
CCTGGCATCC CCCCACCCCC TCCTCCCCTG CCTGGAGGTC CTGGCCTCCC CCCACCCCCT 
CCTCCCCTGC CTGGAGGTCC TGGCATCCCC CCACCCCCTC CTCCCCTGCC TGGAGGTCCT 
GGTCTTCCCC CACCCCCTCC TCCCTTGCCT GGAGGACCAG GAATGCTACC TCCCCCTCCC 
CCTCTTCCTG GTGGAATCCC TCCACCCCCT CCATTTCCTG GAGGCCCTGG CATTCCTCCA 
CCTCTCCCTT TTGGAGTTCC TGCAGCCCCA GTTCTGCCAT TTGGATTAAC CCCAAAGAAA 
CTTTACAAGC CAGAGGTGCA GCTCCGGAGG CCAAACTGGT CCAAGTTTGT CGCTGAGGAC 
CTTTCCCAGG ACTGCTTCTG GACAAAGGTG AAGGAGGACC GATTTGAGAA CAGTGAACTT 
TTCGCCAAAC TTACCAGTAC CTTTTCTGCC CAGACCAAGA CTTCAAAAGC CAAGAAGGAT 
CAGGAAGGTG GAGAAGAAAA GAAATCTCTG CAAAAGAAAA AAGTGAAAGA GTTAAAGGTG 
TTGGATTCCA AGACAGCCCA GAATCTCTCA ATCTTTTTGG GTTCCTTCCG CATGCCCTAT 
CATGAGATCA AGAATGTCAT CCTGGAGGTG AATGAGTCTG TTCTCACTGA GTCCATGATC 
CAGAACCTCA TTAAGCAGAT GCCAGAGCCA GAGCAGTTAA AAATGCTTTC TGAACTGAAG 
GACGAATATG ATGATTTGGC TGAGTCAGAG CAGTTTGGTG TGGTGATGGG TGCAGTACCC 
CGCCTGCGGC CTCGCCTCAA TGCCATCCTC TTCAAGCTGC AGTTCGGGGA GCAGGTGGAG 
AACATCAAGC CCGAGATCGT CTCGGTGACT GCCGCATGTG AGGAGGTGCG AAAGAGCGAG 
AACTTCTCTA ACCTCCTGGA GATAACCTTG CTTGTCGGAA ACTATATGAA TGCTGGCTCC 
AGGAATGCTG GTGCTTTTGG CTTCAACATC AGCTTCCTTT GTAAGCTTCG AGACACCAAG 
TCCACAGATC AGAAGATGAC GTTGTTGCAC TTCTTGGCTG ACTTGTGTGA GCATGATCAT 
CCCGAGGTCC TGAAGTTCCC GGACGAGCTT GCCCACGTGG AGAAAGCCAG CCGAGTTTCT 
GCAGAAAACT TGCAAAAGAA CCTAGATCAG ATGAAGAAAC AGATTTCCGA TGTGGAACGT 
GATATTCAGA ATTTTCCAGC TGCCACAGAA GAGAAAGACA AGTTTGTTGA AAAAATGACC 
AGCTTTGTGA AGGATGCACA GGAACAGTAC AACAAGCTGC GGATGATGCA CTCTAACATG 
GAGATGCTCT ACAAGGAGCT GGGCGAGTAC TTCCTCTTTG ACCCCAAGAA GGTGTCTGTG 
GAAGAATTCT TCATGGATCT GCACAACTTT AAGAATATGT TTGTGCAAGC CGTCAAGGAG 
AACCAGAAGC GGCGGGAGAC AGAGGAGAAG ATGCGGCGAG CAAAGCTGGC CAAGGAGAAG 
GCAGAGAAGG AGCGGTTGGA GAAGCAGCAG AAGAGAGAGC AACTCATAGA CATGAATGCT 
GAGGGTGATG AGACGGGTGT GATGGACAGT CTTCTGGAAG CCCTGCAGTC AGGCGCAGCA 
TTCCGACGGA AGAGAGGACC CCGTCAGGGC GCCAGGAAGG CAGCGTGTGC AGCCACATCT 
CAGCTAGTCT CAGAGCTGAC CAAGGAGGAT GCCATGACCT GTGTTCCTGT CAAGATGCCT 
AAGAAGGAGG AAGTCCCCAC CATCCTCGAG GAGACCACAG AGTTGCTTGG CCGTGCAAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011982405.1
CDS	88..3870
Translation	MEPPGGGLGPGRGTRDKKKGRNPDELPSAGGDGGKSKKFLERFTSMRSKKEKEKPNSAHRNSSAFYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMSQKESSRSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGGYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPSMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFSEIFQILLNTVKDSKAEQHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIDIEGLIDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMESDFEQKLQDIQGEKDSLDSEKQKIATEKQDLEAEVSQLTGEVAKLSKELEDAKKEVASLSAAVTAVAPPSSATVTPAPSLPGDSGPVIPPLSVPTPPLPEASVSLPPPPPAPPLPGCAFISPPGGPSIPPPPPLPGGGGILPPPPAPLPGGPGIPPPPPLPGGPGIPPPPPPLPGGPGLPPPPPPLPGGPGIPPPPPPLPGGPGLPPPPPPLPGGPGMLPPPPPLPGGIPPPPPFPGGPGIPPPLPFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENSELFAKLTSTFSAQTKTSKAKKDQEGGEEKKSLQKKKVKELKVLDSKTAQNLSIFLGSFRMPYHEIKNVILEVNESVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGAVPRLRPRLNAILFKLQFGEQVENIKPEIVSVTAACEEVRKSENFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLADLCEHDHPEVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDIQNFPAATEEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMEMLYKELGEYFLFDPKKVSVEEFFMDLHNFKNMFVQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQGARKAACAATSQLVSELTKEDAMTCVPVKMPKKEEVPTILEETTELLGRAS

Target ORF information:

RefSeq Version	XM_012127015.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon diaphanous related formin 1 (DIAPH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012127015.2

ORF Insert Sequence:

ATGGAGCCGC CCGGCGGGGG CCTGGGACCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAACCCGG ACGAGCTGCC TTCGGCAGGC GGCGACGGCG GCAAATCCAA GAAATTTCTG 
GAGAGATTTA CCAGCATGAG AAGTAAGAAG GAGAAAGAAA AGCCCAATTC TGCTCATAGA 
AACTCTTCTG CATTTTATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT 
GAGCAAGTTC TAGTACTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA CGAGGAGAAA 
CAGCAACCTT TGAGGGAGAA GGACATTGTC ATCAAGAGAG AGATGGTGTC CCAGTACTTG 
CACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA GGTCAGCCAT GATGTACATT 
CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT 
GTCTCCCTCA ACAACAACCC TGTCAGTTGG GTCCAAACAT TTGGTGCTGA GGGCCTGGCC 
TCCTTATTGG ACATCCTTAA ACGACTTCAT GATGAGAAGG AGGAGACTGC TGGAGGCTAC 
GACAGCCGTA ACAAGCATGA AATCATTCGC TGCTTGAAAG CTTTTATGAA TAACAAGTTT 
GGAATCAAGA CCATGCTGGA GACAGAAGAA GGAATTCTGC TGCTGGTCAG AGCCATGGAT 
CCTGCGGTTC CCAGCATGAT GATTGACGCT GCTAAGCTGT TGTCTGCCCT CTGCATCCTG 
CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCGA TGACAGAAAG AGCTGAAATG 
GATGAGGTGG AACGTTTTCA GCCACTGCTG GATGGATTAA AAAGTGGGAC CTCCATTGCT 
CTCAAGGTGG GATGCCTGCA GCTCATCAAT GCTCTCATCA CACCAGCTGA AGAACTTGAC 
TTCCGAGTTC ACATCCGAAG TGAACTGATG CGCTTGGGGC TGCACCAAGT GTTGCAGGAC 
CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAGCTTA ATGTATTTGA TGAACAAGGA 
GAAGAAGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGAATGGA GATGGATGAC 
TTCAGTGAAA TCTTCCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCAGCAC 
TTCCTGTCCA TCCTGCAGCA CTTACTCTTA GTCCGAAATG ACTATGAGGC CAGACCGCAG 
TACTATAAGT TGATTGAAGA ATGTATTTCT CAGATAGTTC TGCATAAGAA CGGGGCTGAT 
CCTGACTTCA AGTGCCGGCA CCTCCAGATT GACATTGAGG GACTGATTGA CCAAATGATT 
GATAAAACAA AGGTGGAGAA ATCAGAAGCC AAAGCTACAG AGCTGGAAAA AAAGCTGGAC 
TCTGAGTTAA CAGCTCGGCA TGAGCTCCAG GTGGAAATGA AAAAGATGGA AAGTGACTTT 
GAGCAGAAGC TCCAGGATAT TCAGGGAGAA AAGGATTCAT TGGATTCTGA AAAGCAAAAA 
ATTGCCACAG AGAAGCAGGA CCTGGAAGCA GAAGTGTCTC AGCTCACAGG AGAGGTCGCC 
AAGCTGTCCA AGGAACTGGA AGATGCCAAG AAAGAAGTGG CTTCTCTCTC TGCTGCAGTT 
ACTGCTGTAG CCCCTCCTAG CAGTGCTACT GTTACCCCTG CCCCTTCTTT ACCTGGCGAC 
TCTGGGCCCG TTATACCCCC CTTATCAGTC CCCACACCCC CTCTTCCTGA GGCCAGTGTC 
AGTCTTCCTC CACCACCTCC TGCTCCTCCT CTACCTGGGT GTGCTTTCAT CTCCCCACCT 
GGGGGTCCTA GCATCCCCCC TCCTCCCCCT TTGCCTGGAG GTGGTGGCAT CCTCCCACCT 
CCTCCTGCCC CTTTGCCTGG AGGTCCTGGC ATCCCCCCAC CTCCTCCCCT TCCTGGAGGT 
CCTGGCATCC CCCCACCCCC TCCTCCCCTG CCTGGAGGTC CTGGCCTCCC CCCACCCCCT 
CCTCCCCTGC CTGGAGGTCC TGGCATCCCC CCACCCCCTC CTCCCCTGCC TGGAGGTCCT 
GGTCTTCCCC CACCCCCTCC TCCCTTGCCT GGAGGACCAG GAATGCTACC TCCCCCTCCC 
CCTCTTCCTG GTGGAATCCC TCCACCCCCT CCATTTCCTG GAGGCCCTGG CATTCCTCCA 
CCTCTCCCTT TTGGAGTTCC TGCAGCCCCA GTTCTGCCAT TTGGATTAAC CCCAAAGAAA 
CTTTACAAGC CAGAGGTGCA GCTCCGGAGG CCAAACTGGT CCAAGTTTGT CGCTGAGGAC 
CTTTCCCAGG ACTGCTTCTG GACAAAGGTG AAGGAGGACC GATTTGAGAA CAGTGAACTT 
TTCGCCAAAC TTACCAGTAC CTTTTCTGCC CAGACCAAGA CTTCAAAAGC CAAGAAGGAT 
CAGGAAGGTG GAGAAGAAAA GAAATCTCTG CAAAAGAAAA AAGTGAAAGA GTTAAAGGTG 
TTGGATTCCA AGACAGCCCA GAATCTCTCA ATCTTTTTGG GTTCCTTCCG CATGCCCTAT 
CATGAGATCA AGAATGTCAT CCTGGAGGTG AATGAGTCTG TTCTCACTGA GTCCATGATC 
CAGAACCTCA TTAAGCAGAT GCCAGAGCCA GAGCAGTTAA AAATGCTTTC TGAACTGAAG 
GACGAATATG ATGATTTGGC TGAGTCAGAG CAGTTTGGTG TGGTGATGGG TGCAGTACCC 
CGCCTGCGGC CTCGCCTCAA TGCCATCCTC TTCAAGCTGC AGTTCGGGGA GCAGGTGGAG 
AACATCAAGC CCGAGATCGT CTCGGTGACT GCCGCATGTG AGGAGGTGCG AAAGAGCGAG 
AACTTCTCTA ACCTCCTGGA GATAACCTTG CTTGTCGGAA ACTATATGAA TGCTGGCTCC 
AGGAATGCTG GTGCTTTTGG CTTCAACATC AGCTTCCTTT GTAAGCTTCG AGACACCAAG 
TCCACAGATC AGAAGATGAC GTTGTTGCAC TTCTTGGCTG ACTTGTGTGA GCATGATCAT 
CCCGAGGTCC TGAAGTTCCC GGACGAGCTT GCCCACGTGG AGAAAGCCAG CCGAGTTTCT 
GCAGAAAACT TGCAAAAGAA CCTAGATCAG ATGAAGAAAC AGATTTCCGA TGTGGAACGT 
GATATTCAGA ATTTTCCAGC TGCCACAGAA GAGAAAGACA AGTTTGTTGA AAAAATGACC 
AGCTTTGTGA AGGATGCACA GGAACAGTAC AACAAGCTGC GGATGATGCA CTCTAACATG 
GAGATGCTCT ACAAGGAGCT GGGCGAGTAC TTCCTCTTTG ACCCCAAGAA GGTGTCTGTG 
GAAGAATTCT TCATGGATCT GCACAACTTT AAGAATATGT TTGTGCAAGC CGTCAAGGAG 
AACCAGAAGC GGCGGGAGAC AGAGGAGAAG ATGCGGCGAG CAAAGCTGGC CAAGGAGAAG 
GCAGAGAAGG AGCGGTTGGA GAAGCAGCAG AAGAGAGAGC AACTCATAGA CATGAATGCT 
GAGGGTGATG AGACGGGTGT GATGGACAGT CTTCTGGAAG CCCTGCAGTC AGGCGCAGCA 
TTCCGACGGA AGAGAGGACC CCGTCAGGGC GCCAGGAAGG CAGCGTGTGC AGCCACATCT 
CAGCTAGTCT CAGAGCTGAC CAAGGAGGAT GCCATGACCT GTGTTCCTGT CAAGATGCCT 
AAGAAGGAGG AAGTCCCCAC CATCCTCGAG GAGACCACAG AGTTGCTTGG CCGTGCAAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( XM_015096089.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014951575.1 Ovis aries diaphanous related formin 1 (DIAPH1), transcript variant X1, mRNA.

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CloneID	OSm49824
Clone ID Related Accession (Same CDS sequence)	XM_015096089.1
Accession Version	XM_015096089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	LOW QUALITY PROTEIN: protein diaphanous homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGAGCCGC CCGGCGGGGG CCTGGGACCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAACCCGG ACGAGCTGCC TTCGGCAGGC GGCGACGGCG GCAAATCCAA GAAATTTACT 
CTGAAGAGAC TCATGGCAGA CGAGCTGGAG AGATTTACCA GCATGAGAAG TAAGAAGGAG 
AAAGAAAAGC CCAATTCTGC TCATAGAAAC TCTTCTGCAT TTTATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAG CAAGTTCTAG TACTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAACGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATTGTCATC 
AAGAGAGAGA TGGTGTCCCA GTACTTGCAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAGGT CAGCCATGAT GTACATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTC TCCCTCAACA ACAACCCTGT CAGTTGGGTC 
CAAACATTTG GTGCTGAGGG CCTGGCCTCC TTATTGGACA TCCTTAAACG ACTTCATGAT 
GAGAAGGAGG AGACTGCTGG AGGCTACGAC AGCCGTAACA AGCATGAAAT CATTCGCTGC 
TTGAAAGCTT TTATGAATAA CAAGTTTGGA ATCAAGACCA TGCTGGAGAC AGAAGAAGGA 
ATTCTGCTGC TGGTCAGAGC CATGGATCCT GCGGTTCCCA GCATGATGAT TGACGCTGCT 
AAGCTGTTGT CTGCCCTCTG CATCCTGCCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCGATGA CAGAAAGAGC TGAAATGGAT GAGGTGGAAC GTTTTCAGCC ACTGCTGGAT 
GGATTAAAAA GTGGGACCTC CATTGCTCTC AAGGTGGGAT GCCTGCAGCT CATCAATGCT 
CTCATCACAC CAGCTGAAGA ACTTGACTTC CGAGTTCACA TCCGAAGTGA ACTGATGCGC 
TTGGGGCTGC ACCAAGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAGCTTAATG TATTTGATGA ACAAGGAGAA GAAGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GAATGGAGAT GGATGACTTC AGTGAAATCT TCCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCAGCACTTC CTGTCCATCC TGCAGCACTT ACTCTTAGTC 
CGAAATGACT ATGAGGCCAG ACCGCAGTAC TATAAGTTGA TTGAAGAATG TATTTCTCAG 
ATAGTTCTGC ATAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAC 
ATTGAGGGAC TGATTGACCA AATGATTGAT AAAACAAAGG TGGAGAAATC AGAAGCCAAA 
GCTACAGAGC TAGAAAAAAA GCTGGACTCT GAGTTAACAG CTCGGCATGA GCTCCAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTCC AGGATATTCA GGGAGAAAAG 
GATTCATTGG ATTCTGAAAA GCAAAAAATT GCCACAGAGA AGCAGGACCT GGAAGCAGAA 
GTGTCTCAGC TCACAGGAGA GGTCGCCAAG CTGTCCAAGG AACTGGAAGA TGCCAAGAAA 
GAAGTGGCTT CTCTCTCTGC TGCAGTTACT GCTGTAGCCC CTCCTAGCAG TGCTACTGTT 
ACCCCTGCCC CTTCTTTACC TGGCGACTCT GGGCCCGTTA TACCCCCCTT ATCAGTCCCC 
ACACCCCCTC TTCCTGAGGC CAGTGTCAGT CTTCCTCCAC CACCTCCTGC TCCTCCTCTA 
CCTGGGTGTG CTTTCATCTC CCCACCTGGG GGTCCTAGCA TCCCCCCTCC TCCCCCTTTG 
CCTGGAGGTG GTGGCATCCT CCCACCTCCT CCTGCCCCTT TGCCTGGAGG TCCTGGCATC 
CCCCCACCTC CTCCCCTTCC TGGAGGTCCT GGCATCCCCC CACCCCCTCC TCCCCTGCCT 
GGAGGTCCTG CTCCCCCACC CCCTCCTCCT CCTGGAGGTC CTGGCATCCC CACCCCTCCT 
CCCCTGCCTG GAGGTCCGGG CATCCCCCAC CCCCCTCCTC CCCTGCCTGG AGGTCCTGGC 
ATCCCCCCAC CCCTCTCCCC TGCCTGGAGT CCTGGTCTTC ATCCCCCCCC CCTCTCTCCT 
TAGCCTGGAG GACCAGGAAT GCTACCTCCC CCTCCCCCTC TTCCTGGTGG AATCCCTCCA 
CCCCCTCCAT TTCCTGGAGG CCCTGGCATT CCTCCACCTC TCCCTTTTGG AGTTCCTGCA 
GCCCCAGTTC TGCCATTTGG ATTAACCCCA AAGAAACTTT ACAAGCCAGA GGTGCAGCTC 
CGGAGGCCAA ACTGGTCCAA GTTTGTCGCT GAGGACCTTT CCCAGGACTG CTTCTGGACA 
AAGGTGAAGG AGGACCGATT TGAGAACAGT GAACTTTTCG CCAAACTTAC CAGTACCTTT 
TCTGCCCAGA CCAAGACTTC AAAAGCCAAG AAGGATCAGG AAGGTGGAGA AGAAAAGAAA 
TCTCTGCAAA AGAAAAAAGT GAAAGAGTTA AAGGTGTTGG ATTCCAAGAC AGCCCAGAAT 
CTCTCAATCT TTTTGGGTTC CTTCCGCATG CCCTATCATG AGATCAAGAA TGTCATCCTG 
GAGGTGAATG AGTCTGTTCT CACTGAGTCC ATGATCCAGA ACCTCATTAA GCAGATGCCA 
GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA CTGAAGGACG AATATGATGA TTTGGCTGAG 
TCAGAGCAGT TTGGTGTGGT GATGGGTGCA GTACCCCGCC TGCGGCCTCG CCTCAATGCC 
ATCCTCTTCA AGCTGCAGTT CGGGGAGCAG GTGGAGAACA TCAAGCCCGA GATCGTCTCG 
GTGACTGCCG CATGTGAGGA GGTGCGAAAG AGCGAGAACT TCTCTAACCT CCTGGAGATA 
ACCTTGCTTG TCGGAAACTA TATGAATGCT GGCTCCAGGA ATGCTGGTGC TTTTGGCTTC 
AACATCAGCT TCCTTTGTAA GCTTCGAGAC ACCAAGTCCA CAGATCAGAA GATGACGTTG 
TTGCACTTCT TGGCTGACTT GTGTGAGCAT GATCATCCCG AGGTCCTGAA GTTCCCGGAC 
GAGCTTGCCC ACGTGGAGAA AGCCAGCCGA GTTTCTGCAG AAAACTTGCA AAAGAACCTA 
GATCAGATGA AGAAACAGAT TTCCGATGTG GAACGTGATA TTCAGAATTT TCCAGCTGCC 
ACAGAAGAGA AAGACAAGTT TGTTGAAAAA ATGACCAGCT TTGTGAAGGA TGCACAGGAA 
CAGTACAACA AGCTGCGGAT GATGCACTCT AACATGGAGA TGCTCTACAA GGAGCTGGGC 
GAGTACTTCC TCTTTGACCC CAAGAAGGTG TCTGTGGAAG AATTCTTCAT GGATCTGCAC 
AACTTTAAGA ATATGTTTGT GCAAGCCGTC AAGGAGAACC AGAAGCGGCG GGAGACAGAG 
GAGAAGATGC GGCGAGCAAA GCTGGCCAAG GAGAAGGCAG AGAAGGAGCG GTTGGAGAAG 
CAGCAGAAGA GAGAGCAACT CATAGACATG AATGCTGAGG GTGATGAGAC GGGTGTGATG 
GACAGTCTTC TGGAAGCCCT GCAGTCAGGC GCAGCATTCC GACGGAAGAG AGGACCCCGT 
CAGGGCGCCA GGAAGGCAGC GTGTGCAGCC ACATCTCAGC TAGTCTCAGA GCTGACCAAG 
GAGGATGCCA TGACCTGTGT TCCTGTCAAG ATGCCTAAGA AGGAGGAAGT CCCCACCATC 
CTCGAGGAGA CCACAGAGTT GCTTGGCCGT GCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014951575.1
CDS	1..3879
Translation	MEPPGGGLGPGRGTRDKKKGRNPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRSKKEKEKPNSAHRNSSAFYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMSQKESSRSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGGYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPSMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFSEIFQILLNTVKDSKAEQHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIDIEGLIDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMESDFEQKLQDIQGEKDSLDSEKQKIATEKQDLEAEVSQLTGEVAKLSKELEDAKKEVASLSAAVTAVAPPSSATVTPAPSLPGDSGPVIPPLSVPTPPLPEASVSLPPPPPAPPLPGCAFISPPGGPSIPPPPPLPGGGGILPPPPAPLPGGPGIPPPPPLPGGPGIPPPPPPLPGGPAPPPPPPPGGPGIPTPPPLPGGPGIPHPPPPLPGGPGIPPPLSPAWSPGLHPPPLSP*PGGPGMLPPPPPLPGGIPPPPPFPGGPGIPPPLPFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENSELFAKLTSTFSAQTKTSKAKKDQEGGEEKKSLQKKKVKELKVLDSKTAQNLSIFLGSFRMPYHEIKNVILEVNESVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGAVPRLRPRLNAILFKLQFGEQVENIKPEIVSVTAACEEVRKSENFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLADLCEHDHPEVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDIQNFPAATEEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMEMLYKELGEYFLFDPKKVSVEEFFMDLHNFKNMFVQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQGARKAACAATSQLVSELTKEDAMTCVPVKMPKKEEVPTILEETTELLGRAS

Target ORF information:

RefSeq Version	XM_015096089.1
Organism	Ovis aries(sheep)
Definition	Ovis aries diaphanous related formin 1 (DIAPH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015096089.1

ORF Insert Sequence:

ATGGAGCCGC CCGGCGGGGG CCTGGGACCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAACCCGG ACGAGCTGCC TTCGGCAGGC GGCGACGGCG GCAAATCCAA GAAATTTACT 
CTGAAGAGAC TCATGGCAGA CGAGCTGGAG AGATTTACCA GCATGAGAAG TAAGAAGGAG 
AAAGAAAAGC CCAATTCTGC TCATAGAAAC TCTTCTGCAT TTTATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAG CAAGTTCTAG TACTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAACGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATTGTCATC 
AAGAGAGAGA TGGTGTCCCA GTACTTGCAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAGGT CAGCCATGAT GTACATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTC TCCCTCAACA ACAACCCTGT CAGTTGGGTC 
CAAACATTTG GTGCTGAGGG CCTGGCCTCC TTATTGGACA TCCTTAAACG ACTTCATGAT 
GAGAAGGAGG AGACTGCTGG AGGCTACGAC AGCCGTAACA AGCATGAAAT CATTCGCTGC 
TTGAAAGCTT TTATGAATAA CAAGTTTGGA ATCAAGACCA TGCTGGAGAC AGAAGAAGGA 
ATTCTGCTGC TGGTCAGAGC CATGGATCCT GCGGTTCCCA GCATGATGAT TGACGCTGCT 
AAGCTGTTGT CTGCCCTCTG CATCCTGCCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCGATGA CAGAAAGAGC TGAAATGGAT GAGGTGGAAC GTTTTCAGCC ACTGCTGGAT 
GGATTAAAAA GTGGGACCTC CATTGCTCTC AAGGTGGGAT GCCTGCAGCT CATCAATGCT 
CTCATCACAC CAGCTGAAGA ACTTGACTTC CGAGTTCACA TCCGAAGTGA ACTGATGCGC 
TTGGGGCTGC ACCAAGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAGCTTAATG TATTTGATGA ACAAGGAGAA GAAGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GAATGGAGAT GGATGACTTC AGTGAAATCT TCCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCAGCACTTC CTGTCCATCC TGCAGCACTT ACTCTTAGTC 
CGAAATGACT ATGAGGCCAG ACCGCAGTAC TATAAGTTGA TTGAAGAATG TATTTCTCAG 
ATAGTTCTGC ATAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAC 
ATTGAGGGAC TGATTGACCA AATGATTGAT AAAACAAAGG TGGAGAAATC AGAAGCCAAA 
GCTACAGAGC TAGAAAAAAA GCTGGACTCT GAGTTAACAG CTCGGCATGA GCTCCAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTCC AGGATATTCA GGGAGAAAAG 
GATTCATTGG ATTCTGAAAA GCAAAAAATT GCCACAGAGA AGCAGGACCT GGAAGCAGAA 
GTGTCTCAGC TCACAGGAGA GGTCGCCAAG CTGTCCAAGG AACTGGAAGA TGCCAAGAAA 
GAAGTGGCTT CTCTCTCTGC TGCAGTTACT GCTGTAGCCC CTCCTAGCAG TGCTACTGTT 
ACCCCTGCCC CTTCTTTACC TGGCGACTCT GGGCCCGTTA TACCCCCCTT ATCAGTCCCC 
ACACCCCCTC TTCCTGAGGC CAGTGTCAGT CTTCCTCCAC CACCTCCTGC TCCTCCTCTA 
CCTGGGTGTG CTTTCATCTC CCCACCTGGG GGTCCTAGCA TCCCCCCTCC TCCCCCTTTG 
CCTGGAGGTG GTGGCATCCT CCCACCTCCT CCTGCCCCTT TGCCTGGAGG TCCTGGCATC 
CCCCCACCTC CTCCCCTTCC TGGAGGTCCT GGCATCCCCC CACCCCCTCC TCCCCTGCCT 
GGAGGTCCTG CTCCCCCACC CCCTCCTCCT CCTGGAGGTC CTGGCATCCC CACCCCTCCT 
CCCCTGCCTG GAGGTCCGGG CATCCCCCAC CCCCCTCCTC CCCTGCCTGG AGGTCCTGGC 
ATCCCCCCAC CCCTCTCCCC TGCCTGGAGT CCTGGTCTTC ATCCCCCCCC CCTCTCTCCT 
TAGCCTGGAG GACCAGGAAT GCTACCTCCC CCTCCCCCTC TTCCTGGTGG AATCCCTCCA 
CCCCCTCCAT TTCCTGGAGG CCCTGGCATT CCTCCACCTC TCCCTTTTGG AGTTCCTGCA 
GCCCCAGTTC TGCCATTTGG ATTAACCCCA AAGAAACTTT ACAAGCCAGA GGTGCAGCTC 
CGGAGGCCAA ACTGGTCCAA GTTTGTCGCT GAGGACCTTT CCCAGGACTG CTTCTGGACA 
AAGGTGAAGG AGGACCGATT TGAGAACAGT GAACTTTTCG CCAAACTTAC CAGTACCTTT 
TCTGCCCAGA CCAAGACTTC AAAAGCCAAG AAGGATCAGG AAGGTGGAGA AGAAAAGAAA 
TCTCTGCAAA AGAAAAAAGT GAAAGAGTTA AAGGTGTTGG ATTCCAAGAC AGCCCAGAAT 
CTCTCAATCT TTTTGGGTTC CTTCCGCATG CCCTATCATG AGATCAAGAA TGTCATCCTG 
GAGGTGAATG AGTCTGTTCT CACTGAGTCC ATGATCCAGA ACCTCATTAA GCAGATGCCA 
GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA CTGAAGGACG AATATGATGA TTTGGCTGAG 
TCAGAGCAGT TTGGTGTGGT GATGGGTGCA GTACCCCGCC TGCGGCCTCG CCTCAATGCC 
ATCCTCTTCA AGCTGCAGTT CGGGGAGCAG GTGGAGAACA TCAAGCCCGA GATCGTCTCG 
GTGACTGCCG CATGTGAGGA GGTGCGAAAG AGCGAGAACT TCTCTAACCT CCTGGAGATA 
ACCTTGCTTG TCGGAAACTA TATGAATGCT GGCTCCAGGA ATGCTGGTGC TTTTGGCTTC 
AACATCAGCT TCCTTTGTAA GCTTCGAGAC ACCAAGTCCA CAGATCAGAA GATGACGTTG 
TTGCACTTCT TGGCTGACTT GTGTGAGCAT GATCATCCCG AGGTCCTGAA GTTCCCGGAC 
GAGCTTGCCC ACGTGGAGAA AGCCAGCCGA GTTTCTGCAG AAAACTTGCA AAAGAACCTA 
GATCAGATGA AGAAACAGAT TTCCGATGTG GAACGTGATA TTCAGAATTT TCCAGCTGCC 
ACAGAAGAGA AAGACAAGTT TGTTGAAAAA ATGACCAGCT TTGTGAAGGA TGCACAGGAA 
CAGTACAACA AGCTGCGGAT GATGCACTCT AACATGGAGA TGCTCTACAA GGAGCTGGGC 
GAGTACTTCC TCTTTGACCC CAAGAAGGTG TCTGTGGAAG AATTCTTCAT GGATCTGCAC 
AACTTTAAGA ATATGTTTGT GCAAGCCGTC AAGGAGAACC AGAAGCGGCG GGAGACAGAG 
GAGAAGATGC GGCGAGCAAA GCTGGCCAAG GAGAAGGCAG AGAAGGAGCG GTTGGAGAAG 
CAGCAGAAGA GAGAGCAACT CATAGACATG AATGCTGAGG GTGATGAGAC GGGTGTGATG 
GACAGTCTTC TGGAAGCCCT GCAGTCAGGC GCAGCATTCC GACGGAAGAG AGGACCCCGT 
CAGGGCGCCA GGAAGGCAGC GTGTGCAGCC ACATCTCAGC TAGTCTCAGA GCTGACCAAG 
GAGGATGCCA TGACCTGTGT TCCTGTCAAG ATGCCTAAGA AGGAGGAAGT CCCCACCATC 
CTCGAGGAGA CCACAGAGTT GCTTGGCCGT GCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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DIAPH1 cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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