LOC112275748 cDNA ORF clone,

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gRNAs

The following LOC112275748 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC112275748 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPn14493	XM_024506367.1 Latest version!	Physcomitrella patens ATP-dependent zinc metalloprotease FTSH 2, chloroplastic-like (LOC112275748), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC112275748 ( XM_024506367.1 ) cDNA ORF clone, -> XP_024362135.1 Physcomitrella patens ATP-dependent zinc metalloprotease FTSH 2, chloroplastic-like (LOC112275748), mRNA.

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CloneID	OPn14493
Clone ID Related Accession (Same CDS sequence)	XM_024506367.1
Accession Version	XM_024506367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	ATP-dependent zinc metalloprotease FTSH 2, chloroplastic-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037275.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCAG CTGCAGGGAT GGCGAAGCTG CTGCCATCTC CTGCCCTCGG CCTTACGAAA 
GAATTGACTG GGTTAAGGCT CTCATCCTCG CGTGCAAGCC CTCGCAGCAT TTCTGCAACC 
CATGTTGTGA GAGCAACTTT GAACACTCAG GAGAATGACA GTACCGGCGA GCTGATTGTT 
CAGAATAGGA GAGCTTTGAT GAAGGGAGGA CTTGCAGCTA TGGGACTATT CCTCCCTTCG 
TTAAAGATGC AATCCGCCAA AGCTGCAGAC GAATCTGAAG GTGTCGCATC CTCCCGCATG 
TCTTACTCAC GATTTTTAGA GTATCTTGAC CAAGGGCGTG TGAAGAAAGT AGATCTGTAC 
GAGAATGGCA CCATAGCCAT TGTGGAGACT GTCTCTCCCG AGCTTGGCAA CCGCGTGCAA 
CGGGTTAGGG TTCAGCTCCC TGGCACAAGC CAAGAGCTCT TGTCAAGATT CCGAGCTAAA 
AACATTGATT TCGCAGCCCA CAATCCTCAG GAGGATCCCG GAAACGTGTT CTTCAACATT 
TTGGGCAACT TGGCATTCCC CCTTATTCTG GTCGGCACCT TATTTTTCCT CAACAGGAAC 
CAAGGGGGAC TTGGTGGACC TGGTGGGCCA GGTAATCCCC TTGCTTTTGG GAAATCCAAG 
GCCAAGTTTC AAATGGAGCC CAATACAGGG ATCACTTTCA AGGATGTAGC AGGTGTGGAT 
GAAGCGAAGC AAGACTTTGT GGAAGTGGTG GATTTTCTTA AGAGACCGGA GCGGTTCACC 
TCTGTTGGTG CAAGAATCCC TAAGGGAGTG TTGCTTGTTG GCCCTCCTGG AACAGGAAAA 
ACTCTCTTGG CCAAGGCCAT TGCTGGAGAG GCGGGAGTTC CATTTTTCTC AATCTCAGGT 
TCTGAATTCG TCGAGATGTT CGTGGGAGTT GGTGCATCGC GAGTACGTGA CCTCTTCAAG 
AAAGCCAAAG AGAATGCTCC TTGTATTGTC TTTGTGGACG AGATCGATGC TGTCGGACGG 
CAAAGGGGTA CTGGCATTGG TGGTGGCAAC GATGAACGAG AACAAACCTT GAACCAGCTC 
CTGACAGAAA TGGATGGATT TGAGGGCAAC ACAGGTGTTA TAGTTATTGC TGCTACCAAC 
AGAGCTGATA TCCTTGATGC TGCTTTATTG CGACCCGGTC GCTTTGATCG TCAGGTTACG 
GTGGATGTGC CTGACGTGAG AGGGAGGACC GAAATCTTGA AAGTACATGC GAGTAACAAG 
AAGTTCGATG ATGATGTGTC GCTTGACATT ATTGCCATGC GCACTCCAGG GTTTAGTGGA 
GCCGATCTCG CTAATCTCTT GAATGAGGCA GCAATATTGA CTGGAAGAAG AGGAAAGACA 
GCTATTTCTG CCAAGGAGAT CGATGATTCC ATTGATAGAA TCGTGGCTGG TATGGAGGGG 
ACAGTCATGA CCGATGGCAA AGCAAAGAGT CTTGTCGCAT ATCATGAAGT TGGCCATGCT 
ATTTGCGGGA CCTTGACACC AGGCCATGAT GCAGTGCAAA AGGTTACCCT GATTCCTAGG 
GGTCAAGCTC GTGGTCTCAC CTGGTTCATT CCAGGCGAGG ACCCCACCCT CATCACCAAG 
CAGCAAATCT TTGCTCGCAT AGTTGGGGCT CTTGGTGGAC GCGCTGCAGA AGAAGTCATC 
TTCGGTGACG CTGAAGTAAC AACTGGCGCT TCCAGTGATT TGCAACAAGT GTCTTCCATG 
GCGAAGCAGA TGGTCACAGT CTACGGCATG TCCGACATCG GCCCGTGGGC ACTTATGGAC 
CCATCTGCCC AAGGTGGAGA TATGATCATG CGCATGATGG CTCGCAACCA GATGTCTGAG 
AAGCTGGCCG AGGATATCGA CCGAGCTGTG AAGAGAATTT CTGACGAAGC CTACAACGTT 
GCTCTCAAGC ACATTAGGGA AAACCGAGTA GCAATGGACA AGATTGTGGA GATCCTTCTG 
GAGAAGGAGA CAATCAGTGG CGATGAGTTC CGAGCTATCT TGTCTGAATA TACTGAGATT 
CCGTCCAGCA ACTCGTCTAA AGATAACCAG TCAGAGCCTG TGGCTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024362135.1
CDS	260..2350
Translation	MAAAAGMAKLLPSPALGLTKELTGLRLSSSRASPRSISATHVVRATLNTQENDSTGELIVQNRRALMKGGLAAMGLFLPSLKMQSAKAADESEGVASSRMSYSRFLEYLDQGRVKKVDLYENGTIAIVETVSPELGNRVQRVRVQLPGTSQELLSRFRAKNIDFAAHNPQEDPGNVFFNILGNLAFPLILVGTLFFLNRNQGGLGGPGGPGNPLAFGKSKAKFQMEPNTGITFKDVAGVDEAKQDFVEVVDFLKRPERFTSVGARIPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGTGIGGGNDEREQTLNQLLTEMDGFEGNTGVIVIAATNRADILDAALLRPGRFDRQVTVDVPDVRGRTEILKVHASNKKFDDDVSLDIIAMRTPGFSGADLANLLNEAAILTGRRGKTAISAKEIDDSIDRIVAGMEGTVMTDGKAKSLVAYHEVGHAICGTLTPGHDAVQKVTLIPRGQARGLTWFIPGEDPTLITKQQIFARIVGALGGRAAEEVIFGDAEVTTGASSDLQQVSSMAKQMVTVYGMSDIGPWALMDPSAQGGDMIMRMMARNQMSEKLAEDIDRAVKRISDEAYNVALKHIRENRVAMDKIVEILLEKETISGDEFRAILSEYTEIPSSNSSKDNQSEPVAV

Target ORF information:

RefSeq Version	XM_024506367.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens ATP-dependent zinc metalloprotease FTSH 2, chloroplastic-like (LOC112275748), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024506367.1

ORF Insert Sequence:

ATGGCGGCAG CTGCAGGGAT GGCGAAGCTG CTGCCATCTC CTGCCCTCGG CCTTACGAAA 
GAATTGACTG GGTTAAGGCT CTCATCCTCG CGTGCAAGCC CTCGCAGCAT TTCTGCAACC 
CATGTTGTGA GAGCAACTTT GAACACTCAG GAGAATGACA GTACCGGCGA GCTGATTGTT 
CAGAATAGGA GAGCTTTGAT GAAGGGAGGA CTTGCAGCTA TGGGACTATT CCTCCCTTCG 
TTAAAGATGC AATCCGCCAA AGCTGCAGAC GAATCTGAAG GTGTCGCATC CTCCCGCATG 
TCTTACTCAC GATTTTTAGA GTATCTTGAC CAAGGGCGTG TGAAGAAAGT AGATCTGTAC 
GAGAATGGCA CCATAGCCAT TGTGGAGACT GTCTCTCCCG AGCTTGGCAA CCGCGTGCAA 
CGGGTTAGGG TTCAGCTCCC TGGCACAAGC CAAGAGCTCT TGTCAAGATT CCGAGCTAAA 
AACATTGATT TCGCAGCCCA CAATCCTCAG GAGGATCCCG GAAACGTGTT CTTCAACATT 
TTGGGCAACT TGGCATTCCC CCTTATTCTG GTCGGCACCT TATTTTTCCT CAACAGGAAC 
CAAGGGGGAC TTGGTGGACC TGGTGGGCCA GGTAATCCCC TTGCTTTTGG GAAATCCAAG 
GCCAAGTTTC AAATGGAGCC CAATACAGGG ATCACTTTCA AGGATGTAGC AGGTGTGGAT 
GAAGCGAAGC AAGACTTTGT GGAAGTGGTG GATTTTCTTA AGAGACCGGA GCGGTTCACC 
TCTGTTGGTG CAAGAATCCC TAAGGGAGTG TTGCTTGTTG GCCCTCCTGG AACAGGAAAA 
ACTCTCTTGG CCAAGGCCAT TGCTGGAGAG GCGGGAGTTC CATTTTTCTC AATCTCAGGT 
TCTGAATTCG TCGAGATGTT CGTGGGAGTT GGTGCATCGC GAGTACGTGA CCTCTTCAAG 
AAAGCCAAAG AGAATGCTCC TTGTATTGTC TTTGTGGACG AGATCGATGC TGTCGGACGG 
CAAAGGGGTA CTGGCATTGG TGGTGGCAAC GATGAACGAG AACAAACCTT GAACCAGCTC 
CTGACAGAAA TGGATGGATT TGAGGGCAAC ACAGGTGTTA TAGTTATTGC TGCTACCAAC 
AGAGCTGATA TCCTTGATGC TGCTTTATTG CGACCCGGTC GCTTTGATCG TCAGGTTACG 
GTGGATGTGC CTGACGTGAG AGGGAGGACC GAAATCTTGA AAGTACATGC GAGTAACAAG 
AAGTTCGATG ATGATGTGTC GCTTGACATT ATTGCCATGC GCACTCCAGG GTTTAGTGGA 
GCCGATCTCG CTAATCTCTT GAATGAGGCA GCAATATTGA CTGGAAGAAG AGGAAAGACA 
GCTATTTCTG CCAAGGAGAT CGATGATTCC ATTGATAGAA TCGTGGCTGG TATGGAGGGG 
ACAGTCATGA CCGATGGCAA AGCAAAGAGT CTTGTCGCAT ATCATGAAGT TGGCCATGCT 
ATTTGCGGGA CCTTGACACC AGGCCATGAT GCAGTGCAAA AGGTTACCCT GATTCCTAGG 
GGTCAAGCTC GTGGTCTCAC CTGGTTCATT CCAGGCGAGG ACCCCACCCT CATCACCAAG 
CAGCAAATCT TTGCTCGCAT AGTTGGGGCT CTTGGTGGAC GCGCTGCAGA AGAAGTCATC 
TTCGGTGACG CTGAAGTAAC AACTGGCGCT TCCAGTGATT TGCAACAAGT GTCTTCCATG 
GCGAAGCAGA TGGTCACAGT CTACGGCATG TCCGACATCG GCCCGTGGGC ACTTATGGAC 
CCATCTGCCC AAGGTGGAGA TATGATCATG CGCATGATGG CTCGCAACCA GATGTCTGAG 
AAGCTGGCCG AGGATATCGA CCGAGCTGTG AAGAGAATTT CTGACGAAGC CTACAACGTT 
GCTCTCAAGC ACATTAGGGA AAACCGAGTA GCAATGGACA AGATTGTGGA GATCCTTCTG 
GAGAAGGAGA CAATCAGTGG CGATGAGTTC CGAGCTATCT TGTCTGAATA TACTGAGATT 
CCGTCCAGCA ACTCGTCTAA AGATAACCAG TCAGAGCCTG TGGCTGTTTG A