LOC112281321 cDNA ORF clone,

Gene
Clones
gRNAs

The following LOC112281321 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC112281321 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPn46215	XM_024517700.1 Latest version!	Physcomitrella patens callose synthase 11-like (LOC112281321), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn46215	XM_024517701.1 Latest version!	Physcomitrella patens callose synthase 11-like (LOC112281321), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn46216	XM_024517702.1 Latest version!	Physcomitrella patens callose synthase 11-like (LOC112281321), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC112281321 ( XM_024517701.1 ) cDNA ORF clone, -> XP_024373469.1 Physcomitrella patens callose synthase 11-like (LOC112281321), transcript variant X2, mRNA.

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CloneID	OPn46215
Clone ID Related Accession (Same CDS sequence)	XM_024517701.1 , XM_024517700.1
Accession Version	XM_024517701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	callose synthase 11-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACC CGGATATGGC TTATCCAGTG TACAACATCT TGCCGGTGGA TGATCCATCA 
GCTGACCATG CGGGGATGTC ATTTCCTGAG GTAAAAGCAG CAATTACGGC TTTGAAAAAG 
GTTGACGATT TGCCTATGCC ACCAGATGTA GCCTGGACAC CTGATATGGA CATGCTGAGT 
TGGCTTGGTA GTTTCTTTGG TTTCCAGGCA GATAATGTGA AGAATCAGCG GGAGCATCTT 
GTCCTGCTTC TTTCGAATGG AATGATGCAG TTGTATCATG CTGGGCCAAC CTTTGAAACA 
CTGGAAGCTA GTATTGTTAG AAAGACCCGA AAGAAGGTGA CGGAGAATTA TGTTAGTTGG 
TGCAAATTTA TCGGACGCAA ACACCACTTG AAGTTGCCAG ATGGAAAGCA TACTGAACAT 
TTTGACGAAA GACGGGAGTT GATCTATATA TGTTTGTACC TTCTAATTTG GGGAGAAGCA 
GCCAACTTGA GATTCATGCC CGAGTGTTTG TGTTTCATCT ATCACCACAT GCTCGGCGAA 
CTGAATAGGT TACTAGAATT TTCTGGAGCA GATGACGTGC TTGCTGTGAT GCCTACCTAC 
ACGGGAGTTA ATGGTTTTCT TAATCATGTT GTTGTTCCTC TCTATACTAT TTTGAAATTG 
GAGGCGGATT CTAATAATAA TGGGACAGCC CCTCACTCTT CATGGCGAAA CTATGACGAT 
CTTAATGAAT ATTTTTGGAC TTCACGTTGT TTCAAACAGC TTCAATGGCC TCTGCAAACT 
AAGAGTAGTT ATCTTGTTGA GCCTAGGCGA GAGAAAGGTT ACCTTAGCCG TGGGAGGAAA 
CCGCAAAGTG AAAAGGTGGG GAAAACTGGA TTCGTTGAAC AGCGGTCATT CTGGTATATC 
TTTCGGAGCT TCGATAAGTT GTGGATAGGT TATCTTCTTA TGCTGCAGGC TTCTGTTGTG 
TTGCTGTGGC ACAACGGTGG GGCTCCATGG ATTGAATTGC AAAAGCCAGA TCCTTTCGCA 
CGTTTCATGA GTATATTTAT CAGTTGGGCT CTTCTACGCT TTTTACAAGG ATTGTTGGAT 
GTGGGGAGTC AATATAGCCT GGTTTCAAAA GATACAAAAC TTATTGGTGT TCGCATGGTT 
CTCAAGCTTC TGGTAGCAGC TACATGGGCC ATCCTTTTCA TCATCTATTA CAGAAGGATG 
TGGTGGCAGC GGAACATCGA TCAGTACTGG ACTGAAATTG CAAACCAAAA GTTGTATGAG 
TTCTTGTACA TTGCTGCAGC CTTTATTGTA CCGGAGGTCC TAGCTATTTT ACTATTTATT 
GTACCATGGG TGCGTAATTT CGTTGAGACC TCAACTTGGA AAGTATTTCA TCTCATGACT 
TGGTGGTTTC AGTCACGTGG TTATGTTGGT CGAGGTCTTC GTGAAGGTAT CATGGACAAC 
GTGAGGTATA CCCTATTTTG GGCATGCGTC CTTACATCCA AATTCGCTTT CAGCTATTGG 
CTCCAGATTA GACCTTTAAT TGCACCAACA AAACAGATCC TTGAAGCCAC CGACGTCAGA 
TACAAGTGGC ACGAGTTTTT TCCAGATGGT AATCGAGCGG CTGTAGTTGC GCTGTGGGCT 
CCTGTTCTTA TGATCTATTT CATGGATACT CAAATATGGT ATTCAATCTG GTCCTCTGGG 
ATTGGAGCTT TTGTTGGTCT CCTTCAACAC CTTGGGGAGA TTCGGAACGT GGAGCAGCTC 
CAACTAAGAT TTCAGATCTT TCCAAGTGCA TTTCAATTCA GTTTAATGCC AGTAGACGAC 
TCTGTAACGA GGACTGTGTG GGCAGGTGCA AAAGACTTGC TTAAGCGGCT TAGCTTGAGA 
TATGGGTGGT CATCCGTTTA TGACAAGATG GAATGGGGTC AAATTGAAGG TGGACGTTTT 
GCCCATGTTT GGAACGAGAT TATTAAAACC TTTCGCGAAG AGGATCTCAT AAGTGACAGA 
GAGGTGGAGC TTATGGAAAT TCCTCAAGGA GCATGGAGGG TCTCCGTTTT TCAATGGCCT 
TCAACCTTGT TGGCTAACCA AATTCTGCTT GCCCTATACA GTATTCGTTA CCATCGAGGA 
GACGATAAAT CTGTTTGGAA CATAATATGT AAAAATGAGT ATAGGAAGTG TGCAGTGGTC 
GAAAGCTATG AGAGCATGAA GCATGTCATC AGAAAGATTC TCAAGGATGA TTCTGATGAG 
TTCCATATTT TTATAGCTAT TTTTGAAGAA ATAGATTTCG CGATAAGGAA GGATAGGTTC 
ACCGAAACTT TCAAGCTTCC AGAGCTGATG GAAATTCATG CCCGAGTTGT TGAATTGATT 
AGTTTTCTTT TGACTCGTCC CGCTGAAAAG CACAAACAGA AGGTAGTGAA AGACCTTCAG 
AACTTGTACG AAGGTCTGCT ACACGACTTT CCACTACAGC CACACATCTT TCTGGAAAGT 
ATCAAGGCAC GCGCTTCATA TCCCCAAAAT AACAAAGGGA CTGAGCTGTT CATGGACGCT 
GTGGAACTCC CAGATAAAGG TGACGAGCAC TTCTTTAAGA ATTTAAAGAG GCTGCATACC 
ACTCTAAGCA CCAGGGACCC TTTACTGTAT GTTCCGAAGG GACTAGAAGC AAGACGACGA 
ATCTCTTTTT TCAGTAACTC GTTGTTCATG ACCATGCCGC GTGCACCACA GGTTGAAAGA 
ATGCTGGCTT TCAGTGTTTT GACACCTTAC TACAATGAGG AAGTTATTTT CAGTAAGCAT 
CAGTTGAAAG AGGAAAACGA GGATGGCGTG ACCATTCTGT TCTACCTTCA ACGGATCTTT 
CCAGAGGACT GGTTAAATTT CCTGGAACGG ATGAAGAAGC TCGAACTTAA TGAGAGTGAG 
TTATGGGAAA AGGATGACGC TCTTGAACTT AGACTTTGGG CTTCTTTTAG AGGACAGACA 
CTTGCGCGAA CTGTACGAGG AATGATGTAT TATAAAAGGG CATTAGAAGT GCAGACCTTT 
CTGGACTCAG CAACTGAAGA TGAGCTACTT GGCATAAAGG AACTTCTAGA ACGAGGCTCT 
AGCACAAACA GCAGAGGTTC TATGCGCTCC ATAGGTTCTA TGGGTTCTAT TGGTTCTGAG 
CTTGAAGTTG CAGAGTTAAA TCGACAACGC AAGTTAGAAC AGGATCTTGC AAATGCTGCA 
ATGAAGTTTA CATACGTGGT AACCTGTCAA ATATACGGGG CTCAAAAGAA GGCCAATGAT 
GTTCGTGCAG CAGATATACT GCGATTAATG AAGACTCATA CAGGACTACG GATTGCCTAT 
GTGGATGAAA GATCTGAATC ATATTTTGAT GAAAATATTG GAGAGTACGT AACGCGTCAA 
CTATATTATT CTGTTTTAGT GAAATATGAT CCAGATTTGA AACAGGAAGT CGAGATTTAT 
CGAATTCGAT TACCTGGTCC TCTGAAGCTT GGAGAAGGGA AGCCAGAAAA TCAGAATCAT 
GCCCTTATAT TTACAAGAGG TGATGCTGTT CAAACTATAG ACATGAATCA GGAAATGTAC 
TTCGAGGAGG CAATAAAGAT GAGAAATCTG CTCCAAGAAT TTACAGTGTA TCATGGAACC 
CGAAAACCCA CAATCTTGGG TGTGCGAGAG CATGTCTTCA CTGGTTCAGT GTCATCACTG 
GCCTGGTTCA TGTCCGCTCA AGAGACAGTG TTTGTCACCC TCAGCCAGCG TGTCCTCGCC 
AATCCTTTGA AGATTCGAAT GCATTATGGT CATCCTGATG TATTTGATCG TTTGTGGTTT 
CTGACTCGCG GCGGGATAAG CAAAGCATCC CGGACTATTA ATATTAGTGA AGATATTTTT 
GCAGGATTTA ATTGTACATT AAGAGGTGGT AATGTAACTC ACCATGAGTA CATTCAGGCT 
GGCAAGGGAA GGGATGTTGG GCTCAACCAA ATCGCTATGT TTGAGGCAAA GGTGGCCAGT 
GGGAATGGTG AACAGATTCT AAGTCGTGAT GTGTATCGCC TTGGTCACCA TTTAGATTTC 
TTTCGCATGC TCTCTTTCTA TTATACCACG GTGGGCTTCT TTGTGAGCAA CATGATGGTT 
GTACTCACTG TCTACACCTT TCTCTGGGGC CGTGTCTACT TGGCTTTAAG CGGCATAGAG 
GAATCCTTAA CATCTGGTTC ACCAGCCCTT GAAAATTCTG CTCTCACAGC GACTTTGAAC 
CAGCAGCTTG TGGTACAGTT GGGGTTGCTG ACTGCTCTAC CCATGGTTGT GGAGGATGCA 
TTAGAGCACG GTTTCACTAC GGCTTTGTGG AATATGATAA CCATGCAGCT TCAACTTGCT 
TCCATCTTTT TCACCTTTTC AATGGGCACT CGTTGTCACT ACTTCGGTCG GACGCTTCTT 
CACGGTGGTG CTAAGTATCG AGCAACTGGT CGTGGCTTTG TAGTTAAGCA TGAAAAGTTT 
GCTGAAAACT ATCGACTTTA CTCCAGAAGC CATTTTGTTA AAGGAATAGA GCTTCTCTTG 
CTGCTTCTGT GTTACTTGGC GTATGGTGTA TCAAGCTCAT CTGGTACATA CATTTTGGTC 
AACATCTCCA GCTGGTTTTT AGCTCTGACA TGGGTAATGG GACCTTTTGT GTTTAATCCG 
TCTGGATTTG ATTGGTTGAA AACTGTTGAA GATTTTGGGG ACTTCATGCA GTGGATATGG 
TTCAAGGGTG ATGTGTTCGT TAAAGTTGAG CAAAGTTGGG AGATATGGTG GGAAGAGGAG 
CAAGCTCATT TACGAACCAC GGGATTGTGG GGGAAGCTAC TAGAGATCGT ACTTGATTTG 
CGATTCTTCA TTTTCCAGTA TGGCATCGTG TACCATTTGG GTATCACAGG GAATAACACA 
AGCATCTTCG TCTACCTTGC ATCATGGAGC TACATGCTTT TTGCAGCCCT TCTCCACTTC 
ATCCTTTCCA ATGCTAATGA GAAGTTAGCT GCCAACAACC ATGGTCTGTA CCGCGCTATT 
CAAGCCCTTG CGATCGCTAT CATTACTGCC TTAGTTGTCG TACTGTGGGT CGTAACCAAC 
TTCACATTTG TGGACATCAT AGCAAGCTTT CTTGCATTCT TACCGACAGG CTGGGGAATC 
ATTCAAATCT GCCTTGTTTT GAGGAGGCCA TTTTTGGAAA ACTCCCCTCT ATGGAGTACC 
ATTGTGGCCG TTGCGCGTCT ATACGATCTT GCCATGGGCA TTATTGTCAT GGCTCCCGTT 
GCTGTGCTGT CGTGGTTGCC AGGATTTCAA GCCATGCAAA CTAGAATACT TTACAACGAA 
GCCTTCAGCA GAGGTCTCCA AATCAGTAGG TTGTTGGCTG GCAAGAGGAA CCGCAATATT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024373469.1
CDS	701..6046
Translation	MENPDMAYPVYNILPVDDPSADHAGMSFPEVKAAITALKKVDDLPMPPDVAWTPDMDMLSWLGSFFGFQADNVKNQREHLVLLLSNGMMQLYHAGPTFETLEASIVRKTRKKVTENYVSWCKFIGRKHHLKLPDGKHTEHFDERRELIYICLYLLIWGEAANLRFMPECLCFIYHHMLGELNRLLEFSGADDVLAVMPTYTGVNGFLNHVVVPLYTILKLEADSNNNGTAPHSSWRNYDDLNEYFWTSRCFKQLQWPLQTKSSYLVEPRREKGYLSRGRKPQSEKVGKTGFVEQRSFWYIFRSFDKLWIGYLLMLQASVVLLWHNGGAPWIELQKPDPFARFMSIFISWALLRFLQGLLDVGSQYSLVSKDTKLIGVRMVLKLLVAATWAILFIIYYRRMWWQRNIDQYWTEIANQKLYEFLYIAAAFIVPEVLAILLFIVPWVRNFVETSTWKVFHLMTWWFQSRGYVGRGLREGIMDNVRYTLFWACVLTSKFAFSYWLQIRPLIAPTKQILEATDVRYKWHEFFPDGNRAAVVALWAPVLMIYFMDTQIWYSIWSSGIGAFVGLLQHLGEIRNVEQLQLRFQIFPSAFQFSLMPVDDSVTRTVWAGAKDLLKRLSLRYGWSSVYDKMEWGQIEGGRFAHVWNEIIKTFREEDLISDREVELMEIPQGAWRVSVFQWPSTLLANQILLALYSIRYHRGDDKSVWNIICKNEYRKCAVVESYESMKHVIRKILKDDSDEFHIFIAIFEEIDFAIRKDRFTETFKLPELMEIHARVVELISFLLTRPAEKHKQKVVKDLQNLYEGLLHDFPLQPHIFLESIKARASYPQNNKGTELFMDAVELPDKGDEHFFKNLKRLHTTLSTRDPLLYVPKGLEARRRISFFSNSLFMTMPRAPQVERMLAFSVLTPYYNEEVIFSKHQLKEENEDGVTILFYLQRIFPEDWLNFLERMKKLELNESELWEKDDALELRLWASFRGQTLARTVRGMMYYKRALEVQTFLDSATEDELLGIKELLERGSSTNSRGSMRSIGSMGSIGSELEVAELNRQRKLEQDLANAAMKFTYVVTCQIYGAQKKANDVRAADILRLMKTHTGLRIAYVDERSESYFDENIGEYVTRQLYYSVLVKYDPDLKQEVEIYRIRLPGPLKLGEGKPENQNHALIFTRGDAVQTIDMNQEMYFEEAIKMRNLLQEFTVYHGTRKPTILGVREHVFTGSVSSLAWFMSAQETVFVTLSQRVLANPLKIRMHYGHPDVFDRLWFLTRGGISKASRTINISEDIFAGFNCTLRGGNVTHHEYIQAGKGRDVGLNQIAMFEAKVASGNGEQILSRDVYRLGHHLDFFRMLSFYYTTVGFFVSNMMVVLTVYTFLWGRVYLALSGIEESLTSGSPALENSALTATLNQQLVVQLGLLTALPMVVEDALEHGFTTALWNMITMQLQLASIFFTFSMGTRCHYFGRTLLHGGAKYRATGRGFVVKHEKFAENYRLYSRSHFVKGIELLLLLLCYLAYGVSSSSGTYILVNISSWFLALTWVMGPFVFNPSGFDWLKTVEDFGDFMQWIWFKGDVFVKVEQSWEIWWEEEQAHLRTTGLWGKLLEIVLDLRFFIFQYGIVYHLGITGNNTSIFVYLASWSYMLFAALLHFILSNANEKLAANNHGLYRAIQALAIAIITALVVVLWVVTNFTFVDIIASFLAFLPTGWGIIQICLVLRRPFLENSPLWSTIVAVARLYDLAMGIIVMAPVAVLSWLPGFQAMQTRILYNEAFSRGLQISRLLAGKRNRNID

Target ORF information:

RefSeq Version	XM_024517701.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens callose synthase 11-like (LOC112281321), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517701.1

ORF Insert Sequence:

ATGGAAAACC CGGATATGGC TTATCCAGTG TACAACATCT TGCCGGTGGA TGATCCATCA 
GCTGACCATG CGGGGATGTC ATTTCCTGAG GTAAAAGCAG CAATTACGGC TTTGAAAAAG 
GTTGACGATT TGCCTATGCC ACCAGATGTA GCCTGGACAC CTGATATGGA CATGCTGAGT 
TGGCTTGGTA GTTTCTTTGG TTTCCAGGCA GATAATGTGA AGAATCAGCG GGAGCATCTT 
GTCCTGCTTC TTTCGAATGG AATGATGCAG TTGTATCATG CTGGGCCAAC CTTTGAAACA 
CTGGAAGCTA GTATTGTTAG AAAGACCCGA AAGAAGGTGA CGGAGAATTA TGTTAGTTGG 
TGCAAATTTA TCGGACGCAA ACACCACTTG AAGTTGCCAG ATGGAAAGCA TACTGAACAT 
TTTGACGAAA GACGGGAGTT GATCTATATA TGTTTGTACC TTCTAATTTG GGGAGAAGCA 
GCCAACTTGA GATTCATGCC CGAGTGTTTG TGTTTCATCT ATCACCACAT GCTCGGCGAA 
CTGAATAGGT TACTAGAATT TTCTGGAGCA GATGACGTGC TTGCTGTGAT GCCTACCTAC 
ACGGGAGTTA ATGGTTTTCT TAATCATGTT GTTGTTCCTC TCTATACTAT TTTGAAATTG 
GAGGCGGATT CTAATAATAA TGGGACAGCC CCTCACTCTT CATGGCGAAA CTATGACGAT 
CTTAATGAAT ATTTTTGGAC TTCACGTTGT TTCAAACAGC TTCAATGGCC TCTGCAAACT 
AAGAGTAGTT ATCTTGTTGA GCCTAGGCGA GAGAAAGGTT ACCTTAGCCG TGGGAGGAAA 
CCGCAAAGTG AAAAGGTGGG GAAAACTGGA TTCGTTGAAC AGCGGTCATT CTGGTATATC 
TTTCGGAGCT TCGATAAGTT GTGGATAGGT TATCTTCTTA TGCTGCAGGC TTCTGTTGTG 
TTGCTGTGGC ACAACGGTGG GGCTCCATGG ATTGAATTGC AAAAGCCAGA TCCTTTCGCA 
CGTTTCATGA GTATATTTAT CAGTTGGGCT CTTCTACGCT TTTTACAAGG ATTGTTGGAT 
GTGGGGAGTC AATATAGCCT GGTTTCAAAA GATACAAAAC TTATTGGTGT TCGCATGGTT 
CTCAAGCTTC TGGTAGCAGC TACATGGGCC ATCCTTTTCA TCATCTATTA CAGAAGGATG 
TGGTGGCAGC GGAACATCGA TCAGTACTGG ACTGAAATTG CAAACCAAAA GTTGTATGAG 
TTCTTGTACA TTGCTGCAGC CTTTATTGTA CCGGAGGTCC TAGCTATTTT ACTATTTATT 
GTACCATGGG TGCGTAATTT CGTTGAGACC TCAACTTGGA AAGTATTTCA TCTCATGACT 
TGGTGGTTTC AGTCACGTGG TTATGTTGGT CGAGGTCTTC GTGAAGGTAT CATGGACAAC 
GTGAGGTATA CCCTATTTTG GGCATGCGTC CTTACATCCA AATTCGCTTT CAGCTATTGG 
CTCCAGATTA GACCTTTAAT TGCACCAACA AAACAGATCC TTGAAGCCAC CGACGTCAGA 
TACAAGTGGC ACGAGTTTTT TCCAGATGGT AATCGAGCGG CTGTAGTTGC GCTGTGGGCT 
CCTGTTCTTA TGATCTATTT CATGGATACT CAAATATGGT ATTCAATCTG GTCCTCTGGG 
ATTGGAGCTT TTGTTGGTCT CCTTCAACAC CTTGGGGAGA TTCGGAACGT GGAGCAGCTC 
CAACTAAGAT TTCAGATCTT TCCAAGTGCA TTTCAATTCA GTTTAATGCC AGTAGACGAC 
TCTGTAACGA GGACTGTGTG GGCAGGTGCA AAAGACTTGC TTAAGCGGCT TAGCTTGAGA 
TATGGGTGGT CATCCGTTTA TGACAAGATG GAATGGGGTC AAATTGAAGG TGGACGTTTT 
GCCCATGTTT GGAACGAGAT TATTAAAACC TTTCGCGAAG AGGATCTCAT AAGTGACAGA 
GAGGTGGAGC TTATGGAAAT TCCTCAAGGA GCATGGAGGG TCTCCGTTTT TCAATGGCCT 
TCAACCTTGT TGGCTAACCA AATTCTGCTT GCCCTATACA GTATTCGTTA CCATCGAGGA 
GACGATAAAT CTGTTTGGAA CATAATATGT AAAAATGAGT ATAGGAAGTG TGCAGTGGTC 
GAAAGCTATG AGAGCATGAA GCATGTCATC AGAAAGATTC TCAAGGATGA TTCTGATGAG 
TTCCATATTT TTATAGCTAT TTTTGAAGAA ATAGATTTCG CGATAAGGAA GGATAGGTTC 
ACCGAAACTT TCAAGCTTCC AGAGCTGATG GAAATTCATG CCCGAGTTGT TGAATTGATT 
AGTTTTCTTT TGACTCGTCC CGCTGAAAAG CACAAACAGA AGGTAGTGAA AGACCTTCAG 
AACTTGTACG AAGGTCTGCT ACACGACTTT CCACTACAGC CACACATCTT TCTGGAAAGT 
ATCAAGGCAC GCGCTTCATA TCCCCAAAAT AACAAAGGGA CTGAGCTGTT CATGGACGCT 
GTGGAACTCC CAGATAAAGG TGACGAGCAC TTCTTTAAGA ATTTAAAGAG GCTGCATACC 
ACTCTAAGCA CCAGGGACCC TTTACTGTAT GTTCCGAAGG GACTAGAAGC AAGACGACGA 
ATCTCTTTTT TCAGTAACTC GTTGTTCATG ACCATGCCGC GTGCACCACA GGTTGAAAGA 
ATGCTGGCTT TCAGTGTTTT GACACCTTAC TACAATGAGG AAGTTATTTT CAGTAAGCAT 
CAGTTGAAAG AGGAAAACGA GGATGGCGTG ACCATTCTGT TCTACCTTCA ACGGATCTTT 
CCAGAGGACT GGTTAAATTT CCTGGAACGG ATGAAGAAGC TCGAACTTAA TGAGAGTGAG 
TTATGGGAAA AGGATGACGC TCTTGAACTT AGACTTTGGG CTTCTTTTAG AGGACAGACA 
CTTGCGCGAA CTGTACGAGG AATGATGTAT TATAAAAGGG CATTAGAAGT GCAGACCTTT 
CTGGACTCAG CAACTGAAGA TGAGCTACTT GGCATAAAGG AACTTCTAGA ACGAGGCTCT 
AGCACAAACA GCAGAGGTTC TATGCGCTCC ATAGGTTCTA TGGGTTCTAT TGGTTCTGAG 
CTTGAAGTTG CAGAGTTAAA TCGACAACGC AAGTTAGAAC AGGATCTTGC AAATGCTGCA 
ATGAAGTTTA CATACGTGGT AACCTGTCAA ATATACGGGG CTCAAAAGAA GGCCAATGAT 
GTTCGTGCAG CAGATATACT GCGATTAATG AAGACTCATA CAGGACTACG GATTGCCTAT 
GTGGATGAAA GATCTGAATC ATATTTTGAT GAAAATATTG GAGAGTACGT AACGCGTCAA 
CTATATTATT CTGTTTTAGT GAAATATGAT CCAGATTTGA AACAGGAAGT CGAGATTTAT 
CGAATTCGAT TACCTGGTCC TCTGAAGCTT GGAGAAGGGA AGCCAGAAAA TCAGAATCAT 
GCCCTTATAT TTACAAGAGG TGATGCTGTT CAAACTATAG ACATGAATCA GGAAATGTAC 
TTCGAGGAGG CAATAAAGAT GAGAAATCTG CTCCAAGAAT TTACAGTGTA TCATGGAACC 
CGAAAACCCA CAATCTTGGG TGTGCGAGAG CATGTCTTCA CTGGTTCAGT GTCATCACTG 
GCCTGGTTCA TGTCCGCTCA AGAGACAGTG TTTGTCACCC TCAGCCAGCG TGTCCTCGCC 
AATCCTTTGA AGATTCGAAT GCATTATGGT CATCCTGATG TATTTGATCG TTTGTGGTTT 
CTGACTCGCG GCGGGATAAG CAAAGCATCC CGGACTATTA ATATTAGTGA AGATATTTTT 
GCAGGATTTA ATTGTACATT AAGAGGTGGT AATGTAACTC ACCATGAGTA CATTCAGGCT 
GGCAAGGGAA GGGATGTTGG GCTCAACCAA ATCGCTATGT TTGAGGCAAA GGTGGCCAGT 
GGGAATGGTG AACAGATTCT AAGTCGTGAT GTGTATCGCC TTGGTCACCA TTTAGATTTC 
TTTCGCATGC TCTCTTTCTA TTATACCACG GTGGGCTTCT TTGTGAGCAA CATGATGGTT 
GTACTCACTG TCTACACCTT TCTCTGGGGC CGTGTCTACT TGGCTTTAAG CGGCATAGAG 
GAATCCTTAA CATCTGGTTC ACCAGCCCTT GAAAATTCTG CTCTCACAGC GACTTTGAAC 
CAGCAGCTTG TGGTACAGTT GGGGTTGCTG ACTGCTCTAC CCATGGTTGT GGAGGATGCA 
TTAGAGCACG GTTTCACTAC GGCTTTGTGG AATATGATAA CCATGCAGCT TCAACTTGCT 
TCCATCTTTT TCACCTTTTC AATGGGCACT CGTTGTCACT ACTTCGGTCG GACGCTTCTT 
CACGGTGGTG CTAAGTATCG AGCAACTGGT CGTGGCTTTG TAGTTAAGCA TGAAAAGTTT 
GCTGAAAACT ATCGACTTTA CTCCAGAAGC CATTTTGTTA AAGGAATAGA GCTTCTCTTG 
CTGCTTCTGT GTTACTTGGC GTATGGTGTA TCAAGCTCAT CTGGTACATA CATTTTGGTC 
AACATCTCCA GCTGGTTTTT AGCTCTGACA TGGGTAATGG GACCTTTTGT GTTTAATCCG 
TCTGGATTTG ATTGGTTGAA AACTGTTGAA GATTTTGGGG ACTTCATGCA GTGGATATGG 
TTCAAGGGTG ATGTGTTCGT TAAAGTTGAG CAAAGTTGGG AGATATGGTG GGAAGAGGAG 
CAAGCTCATT TACGAACCAC GGGATTGTGG GGGAAGCTAC TAGAGATCGT ACTTGATTTG 
CGATTCTTCA TTTTCCAGTA TGGCATCGTG TACCATTTGG GTATCACAGG GAATAACACA 
AGCATCTTCG TCTACCTTGC ATCATGGAGC TACATGCTTT TTGCAGCCCT TCTCCACTTC 
ATCCTTTCCA ATGCTAATGA GAAGTTAGCT GCCAACAACC ATGGTCTGTA CCGCGCTATT 
CAAGCCCTTG CGATCGCTAT CATTACTGCC TTAGTTGTCG TACTGTGGGT CGTAACCAAC 
TTCACATTTG TGGACATCAT AGCAAGCTTT CTTGCATTCT TACCGACAGG CTGGGGAATC 
ATTCAAATCT GCCTTGTTTT GAGGAGGCCA TTTTTGGAAA ACTCCCCTCT ATGGAGTACC 
ATTGTGGCCG TTGCGCGTCT ATACGATCTT GCCATGGGCA TTATTGTCAT GGCTCCCGTT 
GCTGTGCTGT CGTGGTTGCC AGGATTTCAA GCCATGCAAA CTAGAATACT TTACAACGAA 
GCCTTCAGCA GAGGTCTCCA AATCAGTAGG TTGTTGGCTG GCAAGAGGAA CCGCAATATT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281321 ( XM_024517700.1 ) cDNA ORF clone, -> XP_024373468.1 Physcomitrella patens callose synthase 11-like (LOC112281321), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPn46215
Clone ID Related Accession (Same CDS sequence)	XM_024517701.1 , XM_024517700.1
Accession Version	XM_024517700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	callose synthase 11-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACC CGGATATGGC TTATCCAGTG TACAACATCT TGCCGGTGGA TGATCCATCA 
GCTGACCATG CGGGGATGTC ATTTCCTGAG GTAAAAGCAG CAATTACGGC TTTGAAAAAG 
GTTGACGATT TGCCTATGCC ACCAGATGTA GCCTGGACAC CTGATATGGA CATGCTGAGT 
TGGCTTGGTA GTTTCTTTGG TTTCCAGGCA GATAATGTGA AGAATCAGCG GGAGCATCTT 
GTCCTGCTTC TTTCGAATGG AATGATGCAG TTGTATCATG CTGGGCCAAC CTTTGAAACA 
CTGGAAGCTA GTATTGTTAG AAAGACCCGA AAGAAGGTGA CGGAGAATTA TGTTAGTTGG 
TGCAAATTTA TCGGACGCAA ACACCACTTG AAGTTGCCAG ATGGAAAGCA TACTGAACAT 
TTTGACGAAA GACGGGAGTT GATCTATATA TGTTTGTACC TTCTAATTTG GGGAGAAGCA 
GCCAACTTGA GATTCATGCC CGAGTGTTTG TGTTTCATCT ATCACCACAT GCTCGGCGAA 
CTGAATAGGT TACTAGAATT TTCTGGAGCA GATGACGTGC TTGCTGTGAT GCCTACCTAC 
ACGGGAGTTA ATGGTTTTCT TAATCATGTT GTTGTTCCTC TCTATACTAT TTTGAAATTG 
GAGGCGGATT CTAATAATAA TGGGACAGCC CCTCACTCTT CATGGCGAAA CTATGACGAT 
CTTAATGAAT ATTTTTGGAC TTCACGTTGT TTCAAACAGC TTCAATGGCC TCTGCAAACT 
AAGAGTAGTT ATCTTGTTGA GCCTAGGCGA GAGAAAGGTT ACCTTAGCCG TGGGAGGAAA 
CCGCAAAGTG AAAAGGTGGG GAAAACTGGA TTCGTTGAAC AGCGGTCATT CTGGTATATC 
TTTCGGAGCT TCGATAAGTT GTGGATAGGT TATCTTCTTA TGCTGCAGGC TTCTGTTGTG 
TTGCTGTGGC ACAACGGTGG GGCTCCATGG ATTGAATTGC AAAAGCCAGA TCCTTTCGCA 
CGTTTCATGA GTATATTTAT CAGTTGGGCT CTTCTACGCT TTTTACAAGG ATTGTTGGAT 
GTGGGGAGTC AATATAGCCT GGTTTCAAAA GATACAAAAC TTATTGGTGT TCGCATGGTT 
CTCAAGCTTC TGGTAGCAGC TACATGGGCC ATCCTTTTCA TCATCTATTA CAGAAGGATG 
TGGTGGCAGC GGAACATCGA TCAGTACTGG ACTGAAATTG CAAACCAAAA GTTGTATGAG 
TTCTTGTACA TTGCTGCAGC CTTTATTGTA CCGGAGGTCC TAGCTATTTT ACTATTTATT 
GTACCATGGG TGCGTAATTT CGTTGAGACC TCAACTTGGA AAGTATTTCA TCTCATGACT 
TGGTGGTTTC AGTCACGTGG TTATGTTGGT CGAGGTCTTC GTGAAGGTAT CATGGACAAC 
GTGAGGTATA CCCTATTTTG GGCATGCGTC CTTACATCCA AATTCGCTTT CAGCTATTGG 
CTCCAGATTA GACCTTTAAT TGCACCAACA AAACAGATCC TTGAAGCCAC CGACGTCAGA 
TACAAGTGGC ACGAGTTTTT TCCAGATGGT AATCGAGCGG CTGTAGTTGC GCTGTGGGCT 
CCTGTTCTTA TGATCTATTT CATGGATACT CAAATATGGT ATTCAATCTG GTCCTCTGGG 
ATTGGAGCTT TTGTTGGTCT CCTTCAACAC CTTGGGGAGA TTCGGAACGT GGAGCAGCTC 
CAACTAAGAT TTCAGATCTT TCCAAGTGCA TTTCAATTCA GTTTAATGCC AGTAGACGAC 
TCTGTAACGA GGACTGTGTG GGCAGGTGCA AAAGACTTGC TTAAGCGGCT TAGCTTGAGA 
TATGGGTGGT CATCCGTTTA TGACAAGATG GAATGGGGTC AAATTGAAGG TGGACGTTTT 
GCCCATGTTT GGAACGAGAT TATTAAAACC TTTCGCGAAG AGGATCTCAT AAGTGACAGA 
GAGGTGGAGC TTATGGAAAT TCCTCAAGGA GCATGGAGGG TCTCCGTTTT TCAATGGCCT 
TCAACCTTGT TGGCTAACCA AATTCTGCTT GCCCTATACA GTATTCGTTA CCATCGAGGA 
GACGATAAAT CTGTTTGGAA CATAATATGT AAAAATGAGT ATAGGAAGTG TGCAGTGGTC 
GAAAGCTATG AGAGCATGAA GCATGTCATC AGAAAGATTC TCAAGGATGA TTCTGATGAG 
TTCCATATTT TTATAGCTAT TTTTGAAGAA ATAGATTTCG CGATAAGGAA GGATAGGTTC 
ACCGAAACTT TCAAGCTTCC AGAGCTGATG GAAATTCATG CCCGAGTTGT TGAATTGATT 
AGTTTTCTTT TGACTCGTCC CGCTGAAAAG CACAAACAGA AGGTAGTGAA AGACCTTCAG 
AACTTGTACG AAGGTCTGCT ACACGACTTT CCACTACAGC CACACATCTT TCTGGAAAGT 
ATCAAGGCAC GCGCTTCATA TCCCCAAAAT AACAAAGGGA CTGAGCTGTT CATGGACGCT 
GTGGAACTCC CAGATAAAGG TGACGAGCAC TTCTTTAAGA ATTTAAAGAG GCTGCATACC 
ACTCTAAGCA CCAGGGACCC TTTACTGTAT GTTCCGAAGG GACTAGAAGC AAGACGACGA 
ATCTCTTTTT TCAGTAACTC GTTGTTCATG ACCATGCCGC GTGCACCACA GGTTGAAAGA 
ATGCTGGCTT TCAGTGTTTT GACACCTTAC TACAATGAGG AAGTTATTTT CAGTAAGCAT 
CAGTTGAAAG AGGAAAACGA GGATGGCGTG ACCATTCTGT TCTACCTTCA ACGGATCTTT 
CCAGAGGACT GGTTAAATTT CCTGGAACGG ATGAAGAAGC TCGAACTTAA TGAGAGTGAG 
TTATGGGAAA AGGATGACGC TCTTGAACTT AGACTTTGGG CTTCTTTTAG AGGACAGACA 
CTTGCGCGAA CTGTACGAGG AATGATGTAT TATAAAAGGG CATTAGAAGT GCAGACCTTT 
CTGGACTCAG CAACTGAAGA TGAGCTACTT GGCATAAAGG AACTTCTAGA ACGAGGCTCT 
AGCACAAACA GCAGAGGTTC TATGCGCTCC ATAGGTTCTA TGGGTTCTAT TGGTTCTGAG 
CTTGAAGTTG CAGAGTTAAA TCGACAACGC AAGTTAGAAC AGGATCTTGC AAATGCTGCA 
ATGAAGTTTA CATACGTGGT AACCTGTCAA ATATACGGGG CTCAAAAGAA GGCCAATGAT 
GTTCGTGCAG CAGATATACT GCGATTAATG AAGACTCATA CAGGACTACG GATTGCCTAT 
GTGGATGAAA GATCTGAATC ATATTTTGAT GAAAATATTG GAGAGTACGT AACGCGTCAA 
CTATATTATT CTGTTTTAGT GAAATATGAT CCAGATTTGA AACAGGAAGT CGAGATTTAT 
CGAATTCGAT TACCTGGTCC TCTGAAGCTT GGAGAAGGGA AGCCAGAAAA TCAGAATCAT 
GCCCTTATAT TTACAAGAGG TGATGCTGTT CAAACTATAG ACATGAATCA GGAAATGTAC 
TTCGAGGAGG CAATAAAGAT GAGAAATCTG CTCCAAGAAT TTACAGTGTA TCATGGAACC 
CGAAAACCCA CAATCTTGGG TGTGCGAGAG CATGTCTTCA CTGGTTCAGT GTCATCACTG 
GCCTGGTTCA TGTCCGCTCA AGAGACAGTG TTTGTCACCC TCAGCCAGCG TGTCCTCGCC 
AATCCTTTGA AGATTCGAAT GCATTATGGT CATCCTGATG TATTTGATCG TTTGTGGTTT 
CTGACTCGCG GCGGGATAAG CAAAGCATCC CGGACTATTA ATATTAGTGA AGATATTTTT 
GCAGGATTTA ATTGTACATT AAGAGGTGGT AATGTAACTC ACCATGAGTA CATTCAGGCT 
GGCAAGGGAA GGGATGTTGG GCTCAACCAA ATCGCTATGT TTGAGGCAAA GGTGGCCAGT 
GGGAATGGTG AACAGATTCT AAGTCGTGAT GTGTATCGCC TTGGTCACCA TTTAGATTTC 
TTTCGCATGC TCTCTTTCTA TTATACCACG GTGGGCTTCT TTGTGAGCAA CATGATGGTT 
GTACTCACTG TCTACACCTT TCTCTGGGGC CGTGTCTACT TGGCTTTAAG CGGCATAGAG 
GAATCCTTAA CATCTGGTTC ACCAGCCCTT GAAAATTCTG CTCTCACAGC GACTTTGAAC 
CAGCAGCTTG TGGTACAGTT GGGGTTGCTG ACTGCTCTAC CCATGGTTGT GGAGGATGCA 
TTAGAGCACG GTTTCACTAC GGCTTTGTGG AATATGATAA CCATGCAGCT TCAACTTGCT 
TCCATCTTTT TCACCTTTTC AATGGGCACT CGTTGTCACT ACTTCGGTCG GACGCTTCTT 
CACGGTGGTG CTAAGTATCG AGCAACTGGT CGTGGCTTTG TAGTTAAGCA TGAAAAGTTT 
GCTGAAAACT ATCGACTTTA CTCCAGAAGC CATTTTGTTA AAGGAATAGA GCTTCTCTTG 
CTGCTTCTGT GTTACTTGGC GTATGGTGTA TCAAGCTCAT CTGGTACATA CATTTTGGTC 
AACATCTCCA GCTGGTTTTT AGCTCTGACA TGGGTAATGG GACCTTTTGT GTTTAATCCG 
TCTGGATTTG ATTGGTTGAA AACTGTTGAA GATTTTGGGG ACTTCATGCA GTGGATATGG 
TTCAAGGGTG ATGTGTTCGT TAAAGTTGAG CAAAGTTGGG AGATATGGTG GGAAGAGGAG 
CAAGCTCATT TACGAACCAC GGGATTGTGG GGGAAGCTAC TAGAGATCGT ACTTGATTTG 
CGATTCTTCA TTTTCCAGTA TGGCATCGTG TACCATTTGG GTATCACAGG GAATAACACA 
AGCATCTTCG TCTACCTTGC ATCATGGAGC TACATGCTTT TTGCAGCCCT TCTCCACTTC 
ATCCTTTCCA ATGCTAATGA GAAGTTAGCT GCCAACAACC ATGGTCTGTA CCGCGCTATT 
CAAGCCCTTG CGATCGCTAT CATTACTGCC TTAGTTGTCG TACTGTGGGT CGTAACCAAC 
TTCACATTTG TGGACATCAT AGCAAGCTTT CTTGCATTCT TACCGACAGG CTGGGGAATC 
ATTCAAATCT GCCTTGTTTT GAGGAGGCCA TTTTTGGAAA ACTCCCCTCT ATGGAGTACC 
ATTGTGGCCG TTGCGCGTCT ATACGATCTT GCCATGGGCA TTATTGTCAT GGCTCCCGTT 
GCTGTGCTGT CGTGGTTGCC AGGATTTCAA GCCATGCAAA CTAGAATACT TTACAACGAA 
GCCTTCAGCA GAGGTCTCCA AATCAGTAGG TTGTTGGCTG GCAAGAGGAA CCGCAATATT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024373468.1
CDS	629..5974
Translation	MENPDMAYPVYNILPVDDPSADHAGMSFPEVKAAITALKKVDDLPMPPDVAWTPDMDMLSWLGSFFGFQADNVKNQREHLVLLLSNGMMQLYHAGPTFETLEASIVRKTRKKVTENYVSWCKFIGRKHHLKLPDGKHTEHFDERRELIYICLYLLIWGEAANLRFMPECLCFIYHHMLGELNRLLEFSGADDVLAVMPTYTGVNGFLNHVVVPLYTILKLEADSNNNGTAPHSSWRNYDDLNEYFWTSRCFKQLQWPLQTKSSYLVEPRREKGYLSRGRKPQSEKVGKTGFVEQRSFWYIFRSFDKLWIGYLLMLQASVVLLWHNGGAPWIELQKPDPFARFMSIFISWALLRFLQGLLDVGSQYSLVSKDTKLIGVRMVLKLLVAATWAILFIIYYRRMWWQRNIDQYWTEIANQKLYEFLYIAAAFIVPEVLAILLFIVPWVRNFVETSTWKVFHLMTWWFQSRGYVGRGLREGIMDNVRYTLFWACVLTSKFAFSYWLQIRPLIAPTKQILEATDVRYKWHEFFPDGNRAAVVALWAPVLMIYFMDTQIWYSIWSSGIGAFVGLLQHLGEIRNVEQLQLRFQIFPSAFQFSLMPVDDSVTRTVWAGAKDLLKRLSLRYGWSSVYDKMEWGQIEGGRFAHVWNEIIKTFREEDLISDREVELMEIPQGAWRVSVFQWPSTLLANQILLALYSIRYHRGDDKSVWNIICKNEYRKCAVVESYESMKHVIRKILKDDSDEFHIFIAIFEEIDFAIRKDRFTETFKLPELMEIHARVVELISFLLTRPAEKHKQKVVKDLQNLYEGLLHDFPLQPHIFLESIKARASYPQNNKGTELFMDAVELPDKGDEHFFKNLKRLHTTLSTRDPLLYVPKGLEARRRISFFSNSLFMTMPRAPQVERMLAFSVLTPYYNEEVIFSKHQLKEENEDGVTILFYLQRIFPEDWLNFLERMKKLELNESELWEKDDALELRLWASFRGQTLARTVRGMMYYKRALEVQTFLDSATEDELLGIKELLERGSSTNSRGSMRSIGSMGSIGSELEVAELNRQRKLEQDLANAAMKFTYVVTCQIYGAQKKANDVRAADILRLMKTHTGLRIAYVDERSESYFDENIGEYVTRQLYYSVLVKYDPDLKQEVEIYRIRLPGPLKLGEGKPENQNHALIFTRGDAVQTIDMNQEMYFEEAIKMRNLLQEFTVYHGTRKPTILGVREHVFTGSVSSLAWFMSAQETVFVTLSQRVLANPLKIRMHYGHPDVFDRLWFLTRGGISKASRTINISEDIFAGFNCTLRGGNVTHHEYIQAGKGRDVGLNQIAMFEAKVASGNGEQILSRDVYRLGHHLDFFRMLSFYYTTVGFFVSNMMVVLTVYTFLWGRVYLALSGIEESLTSGSPALENSALTATLNQQLVVQLGLLTALPMVVEDALEHGFTTALWNMITMQLQLASIFFTFSMGTRCHYFGRTLLHGGAKYRATGRGFVVKHEKFAENYRLYSRSHFVKGIELLLLLLCYLAYGVSSSSGTYILVNISSWFLALTWVMGPFVFNPSGFDWLKTVEDFGDFMQWIWFKGDVFVKVEQSWEIWWEEEQAHLRTTGLWGKLLEIVLDLRFFIFQYGIVYHLGITGNNTSIFVYLASWSYMLFAALLHFILSNANEKLAANNHGLYRAIQALAIAIITALVVVLWVVTNFTFVDIIASFLAFLPTGWGIIQICLVLRRPFLENSPLWSTIVAVARLYDLAMGIIVMAPVAVLSWLPGFQAMQTRILYNEAFSRGLQISRLLAGKRNRNID

Target ORF information:

RefSeq Version	XM_024517700.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens callose synthase 11-like (LOC112281321), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517700.1

ORF Insert Sequence:

ATGGAAAACC CGGATATGGC TTATCCAGTG TACAACATCT TGCCGGTGGA TGATCCATCA 
GCTGACCATG CGGGGATGTC ATTTCCTGAG GTAAAAGCAG CAATTACGGC TTTGAAAAAG 
GTTGACGATT TGCCTATGCC ACCAGATGTA GCCTGGACAC CTGATATGGA CATGCTGAGT 
TGGCTTGGTA GTTTCTTTGG TTTCCAGGCA GATAATGTGA AGAATCAGCG GGAGCATCTT 
GTCCTGCTTC TTTCGAATGG AATGATGCAG TTGTATCATG CTGGGCCAAC CTTTGAAACA 
CTGGAAGCTA GTATTGTTAG AAAGACCCGA AAGAAGGTGA CGGAGAATTA TGTTAGTTGG 
TGCAAATTTA TCGGACGCAA ACACCACTTG AAGTTGCCAG ATGGAAAGCA TACTGAACAT 
TTTGACGAAA GACGGGAGTT GATCTATATA TGTTTGTACC TTCTAATTTG GGGAGAAGCA 
GCCAACTTGA GATTCATGCC CGAGTGTTTG TGTTTCATCT ATCACCACAT GCTCGGCGAA 
CTGAATAGGT TACTAGAATT TTCTGGAGCA GATGACGTGC TTGCTGTGAT GCCTACCTAC 
ACGGGAGTTA ATGGTTTTCT TAATCATGTT GTTGTTCCTC TCTATACTAT TTTGAAATTG 
GAGGCGGATT CTAATAATAA TGGGACAGCC CCTCACTCTT CATGGCGAAA CTATGACGAT 
CTTAATGAAT ATTTTTGGAC TTCACGTTGT TTCAAACAGC TTCAATGGCC TCTGCAAACT 
AAGAGTAGTT ATCTTGTTGA GCCTAGGCGA GAGAAAGGTT ACCTTAGCCG TGGGAGGAAA 
CCGCAAAGTG AAAAGGTGGG GAAAACTGGA TTCGTTGAAC AGCGGTCATT CTGGTATATC 
TTTCGGAGCT TCGATAAGTT GTGGATAGGT TATCTTCTTA TGCTGCAGGC TTCTGTTGTG 
TTGCTGTGGC ACAACGGTGG GGCTCCATGG ATTGAATTGC AAAAGCCAGA TCCTTTCGCA 
CGTTTCATGA GTATATTTAT CAGTTGGGCT CTTCTACGCT TTTTACAAGG ATTGTTGGAT 
GTGGGGAGTC AATATAGCCT GGTTTCAAAA GATACAAAAC TTATTGGTGT TCGCATGGTT 
CTCAAGCTTC TGGTAGCAGC TACATGGGCC ATCCTTTTCA TCATCTATTA CAGAAGGATG 
TGGTGGCAGC GGAACATCGA TCAGTACTGG ACTGAAATTG CAAACCAAAA GTTGTATGAG 
TTCTTGTACA TTGCTGCAGC CTTTATTGTA CCGGAGGTCC TAGCTATTTT ACTATTTATT 
GTACCATGGG TGCGTAATTT CGTTGAGACC TCAACTTGGA AAGTATTTCA TCTCATGACT 
TGGTGGTTTC AGTCACGTGG TTATGTTGGT CGAGGTCTTC GTGAAGGTAT CATGGACAAC 
GTGAGGTATA CCCTATTTTG GGCATGCGTC CTTACATCCA AATTCGCTTT CAGCTATTGG 
CTCCAGATTA GACCTTTAAT TGCACCAACA AAACAGATCC TTGAAGCCAC CGACGTCAGA 
TACAAGTGGC ACGAGTTTTT TCCAGATGGT AATCGAGCGG CTGTAGTTGC GCTGTGGGCT 
CCTGTTCTTA TGATCTATTT CATGGATACT CAAATATGGT ATTCAATCTG GTCCTCTGGG 
ATTGGAGCTT TTGTTGGTCT CCTTCAACAC CTTGGGGAGA TTCGGAACGT GGAGCAGCTC 
CAACTAAGAT TTCAGATCTT TCCAAGTGCA TTTCAATTCA GTTTAATGCC AGTAGACGAC 
TCTGTAACGA GGACTGTGTG GGCAGGTGCA AAAGACTTGC TTAAGCGGCT TAGCTTGAGA 
TATGGGTGGT CATCCGTTTA TGACAAGATG GAATGGGGTC AAATTGAAGG TGGACGTTTT 
GCCCATGTTT GGAACGAGAT TATTAAAACC TTTCGCGAAG AGGATCTCAT AAGTGACAGA 
GAGGTGGAGC TTATGGAAAT TCCTCAAGGA GCATGGAGGG TCTCCGTTTT TCAATGGCCT 
TCAACCTTGT TGGCTAACCA AATTCTGCTT GCCCTATACA GTATTCGTTA CCATCGAGGA 
GACGATAAAT CTGTTTGGAA CATAATATGT AAAAATGAGT ATAGGAAGTG TGCAGTGGTC 
GAAAGCTATG AGAGCATGAA GCATGTCATC AGAAAGATTC TCAAGGATGA TTCTGATGAG 
TTCCATATTT TTATAGCTAT TTTTGAAGAA ATAGATTTCG CGATAAGGAA GGATAGGTTC 
ACCGAAACTT TCAAGCTTCC AGAGCTGATG GAAATTCATG CCCGAGTTGT TGAATTGATT 
AGTTTTCTTT TGACTCGTCC CGCTGAAAAG CACAAACAGA AGGTAGTGAA AGACCTTCAG 
AACTTGTACG AAGGTCTGCT ACACGACTTT CCACTACAGC CACACATCTT TCTGGAAAGT 
ATCAAGGCAC GCGCTTCATA TCCCCAAAAT AACAAAGGGA CTGAGCTGTT CATGGACGCT 
GTGGAACTCC CAGATAAAGG TGACGAGCAC TTCTTTAAGA ATTTAAAGAG GCTGCATACC 
ACTCTAAGCA CCAGGGACCC TTTACTGTAT GTTCCGAAGG GACTAGAAGC AAGACGACGA 
ATCTCTTTTT TCAGTAACTC GTTGTTCATG ACCATGCCGC GTGCACCACA GGTTGAAAGA 
ATGCTGGCTT TCAGTGTTTT GACACCTTAC TACAATGAGG AAGTTATTTT CAGTAAGCAT 
CAGTTGAAAG AGGAAAACGA GGATGGCGTG ACCATTCTGT TCTACCTTCA ACGGATCTTT 
CCAGAGGACT GGTTAAATTT CCTGGAACGG ATGAAGAAGC TCGAACTTAA TGAGAGTGAG 
TTATGGGAAA AGGATGACGC TCTTGAACTT AGACTTTGGG CTTCTTTTAG AGGACAGACA 
CTTGCGCGAA CTGTACGAGG AATGATGTAT TATAAAAGGG CATTAGAAGT GCAGACCTTT 
CTGGACTCAG CAACTGAAGA TGAGCTACTT GGCATAAAGG AACTTCTAGA ACGAGGCTCT 
AGCACAAACA GCAGAGGTTC TATGCGCTCC ATAGGTTCTA TGGGTTCTAT TGGTTCTGAG 
CTTGAAGTTG CAGAGTTAAA TCGACAACGC AAGTTAGAAC AGGATCTTGC AAATGCTGCA 
ATGAAGTTTA CATACGTGGT AACCTGTCAA ATATACGGGG CTCAAAAGAA GGCCAATGAT 
GTTCGTGCAG CAGATATACT GCGATTAATG AAGACTCATA CAGGACTACG GATTGCCTAT 
GTGGATGAAA GATCTGAATC ATATTTTGAT GAAAATATTG GAGAGTACGT AACGCGTCAA 
CTATATTATT CTGTTTTAGT GAAATATGAT CCAGATTTGA AACAGGAAGT CGAGATTTAT 
CGAATTCGAT TACCTGGTCC TCTGAAGCTT GGAGAAGGGA AGCCAGAAAA TCAGAATCAT 
GCCCTTATAT TTACAAGAGG TGATGCTGTT CAAACTATAG ACATGAATCA GGAAATGTAC 
TTCGAGGAGG CAATAAAGAT GAGAAATCTG CTCCAAGAAT TTACAGTGTA TCATGGAACC 
CGAAAACCCA CAATCTTGGG TGTGCGAGAG CATGTCTTCA CTGGTTCAGT GTCATCACTG 
GCCTGGTTCA TGTCCGCTCA AGAGACAGTG TTTGTCACCC TCAGCCAGCG TGTCCTCGCC 
AATCCTTTGA AGATTCGAAT GCATTATGGT CATCCTGATG TATTTGATCG TTTGTGGTTT 
CTGACTCGCG GCGGGATAAG CAAAGCATCC CGGACTATTA ATATTAGTGA AGATATTTTT 
GCAGGATTTA ATTGTACATT AAGAGGTGGT AATGTAACTC ACCATGAGTA CATTCAGGCT 
GGCAAGGGAA GGGATGTTGG GCTCAACCAA ATCGCTATGT TTGAGGCAAA GGTGGCCAGT 
GGGAATGGTG AACAGATTCT AAGTCGTGAT GTGTATCGCC TTGGTCACCA TTTAGATTTC 
TTTCGCATGC TCTCTTTCTA TTATACCACG GTGGGCTTCT TTGTGAGCAA CATGATGGTT 
GTACTCACTG TCTACACCTT TCTCTGGGGC CGTGTCTACT TGGCTTTAAG CGGCATAGAG 
GAATCCTTAA CATCTGGTTC ACCAGCCCTT GAAAATTCTG CTCTCACAGC GACTTTGAAC 
CAGCAGCTTG TGGTACAGTT GGGGTTGCTG ACTGCTCTAC CCATGGTTGT GGAGGATGCA 
TTAGAGCACG GTTTCACTAC GGCTTTGTGG AATATGATAA CCATGCAGCT TCAACTTGCT 
TCCATCTTTT TCACCTTTTC AATGGGCACT CGTTGTCACT ACTTCGGTCG GACGCTTCTT 
CACGGTGGTG CTAAGTATCG AGCAACTGGT CGTGGCTTTG TAGTTAAGCA TGAAAAGTTT 
GCTGAAAACT ATCGACTTTA CTCCAGAAGC CATTTTGTTA AAGGAATAGA GCTTCTCTTG 
CTGCTTCTGT GTTACTTGGC GTATGGTGTA TCAAGCTCAT CTGGTACATA CATTTTGGTC 
AACATCTCCA GCTGGTTTTT AGCTCTGACA TGGGTAATGG GACCTTTTGT GTTTAATCCG 
TCTGGATTTG ATTGGTTGAA AACTGTTGAA GATTTTGGGG ACTTCATGCA GTGGATATGG 
TTCAAGGGTG ATGTGTTCGT TAAAGTTGAG CAAAGTTGGG AGATATGGTG GGAAGAGGAG 
CAAGCTCATT TACGAACCAC GGGATTGTGG GGGAAGCTAC TAGAGATCGT ACTTGATTTG 
CGATTCTTCA TTTTCCAGTA TGGCATCGTG TACCATTTGG GTATCACAGG GAATAACACA 
AGCATCTTCG TCTACCTTGC ATCATGGAGC TACATGCTTT TTGCAGCCCT TCTCCACTTC 
ATCCTTTCCA ATGCTAATGA GAAGTTAGCT GCCAACAACC ATGGTCTGTA CCGCGCTATT 
CAAGCCCTTG CGATCGCTAT CATTACTGCC TTAGTTGTCG TACTGTGGGT CGTAACCAAC 
TTCACATTTG TGGACATCAT AGCAAGCTTT CTTGCATTCT TACCGACAGG CTGGGGAATC 
ATTCAAATCT GCCTTGTTTT GAGGAGGCCA TTTTTGGAAA ACTCCCCTCT ATGGAGTACC 
ATTGTGGCCG TTGCGCGTCT ATACGATCTT GCCATGGGCA TTATTGTCAT GGCTCCCGTT 
GCTGTGCTGT CGTGGTTGCC AGGATTTCAA GCCATGCAAA CTAGAATACT TTACAACGAA 
GCCTTCAGCA GAGGTCTCCA AATCAGTAGG TTGTTGGCTG GCAAGAGGAA CCGCAATATT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112281321 ( XM_024517702.1 ) cDNA ORF clone, -> XP_024373470.1 Physcomitrella patens callose synthase 11-like (LOC112281321), transcript variant X3, mRNA.

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CloneID	OPn46216
Clone ID Related Accession (Same CDS sequence)	XM_024517702.1
Accession Version	XM_024517702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	callose synthase 11-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAGT GTTTGTGTTT CATCTATCAC CACATGCTCG GCGAACTGAA TAGGTTACTA 
GAATTTTCTG GAGCAGATGA CGTGCTTGCT GTGATGCCTA CCTACACGGG AGTTAATGGT 
TTTCTTAATC ATGTTGTTGT TCCTCTCTAT ACTATTTTGA AATTGGAGGC GGATTCTAAT 
AATAATGGGA CAGCCCCTCA CTCTTCATGG CGAAACTATG ACGATCTTAA TGAATATTTT 
TGGACTTCAC GTTGTTTCAA ACAGCTTCAA TGGCCTCTGC AAACTAAGAG TAGTTATCTT 
GTTGAGCCTA GGCGAGAGAA AGGTTACCTT AGCCGTGGGA GGAAACCGCA AAGTGAAAAG 
GTGGGGAAAA CTGGATTCGT TGAACAGCGG TCATTCTGGT ATATCTTTCG GAGCTTCGAT 
AAGTTGTGGA TAGGTTATCT TCTTATGCTG CAGGCTTCTG TTGTGTTGCT GTGGCACAAC 
GGTGGGGCTC CATGGATTGA ATTGCAAAAG CCAGATCCTT TCGCACGTTT CATGAGTATA 
TTTATCAGTT GGGCTCTTCT ACGCTTTTTA CAAGGATTGT TGGATGTGGG GAGTCAATAT 
AGCCTGGTTT CAAAAGATAC AAAACTTATT GGTGTTCGCA TGGTTCTCAA GCTTCTGGTA 
GCAGCTACAT GGGCCATCCT TTTCATCATC TATTACAGAA GGATGTGGTG GCAGCGGAAC 
ATCGATCAGT ACTGGACTGA AATTGCAAAC CAAAAGTTGT ATGAGTTCTT GTACATTGCT 
GCAGCCTTTA TTGTACCGGA GGTCCTAGCT ATTTTACTAT TTATTGTACC ATGGGTGCGT 
AATTTCGTTG AGACCTCAAC TTGGAAAGTA TTTCATCTCA TGACTTGGTG GTTTCAGTCA 
CGTGGTTATG TTGGTCGAGG TCTTCGTGAA GGTATCATGG ACAACGTGAG GTATACCCTA 
TTTTGGGCAT GCGTCCTTAC ATCCAAATTC GCTTTCAGCT ATTGGCTCCA GATTAGACCT 
TTAATTGCAC CAACAAAACA GATCCTTGAA GCCACCGACG TCAGATACAA GTGGCACGAG 
TTTTTTCCAG ATGGTAATCG AGCGGCTGTA GTTGCGCTGT GGGCTCCTGT TCTTATGATC 
TATTTCATGG ATACTCAAAT ATGGTATTCA ATCTGGTCCT CTGGGATTGG AGCTTTTGTT 
GGTCTCCTTC AACACCTTGG GGAGATTCGG AACGTGGAGC AGCTCCAACT AAGATTTCAG 
ATCTTTCCAA GTGCATTTCA ATTCAGTTTA ATGCCAGTAG ACGACTCTGT AACGAGGACT 
GTGTGGGCAG GTGCAAAAGA CTTGCTTAAG CGGCTTAGCT TGAGATATGG GTGGTCATCC 
GTTTATGACA AGATGGAATG GGGTCAAATT GAAGGTGGAC GTTTTGCCCA TGTTTGGAAC 
GAGATTATTA AAACCTTTCG CGAAGAGGAT CTCATAAGTG ACAGAGAGGT GGAGCTTATG 
GAAATTCCTC AAGGAGCATG GAGGGTCTCC GTTTTTCAAT GGCCTTCAAC CTTGTTGGCT 
AACCAAATTC TGCTTGCCCT ATACAGTATT CGTTACCATC GAGGAGACGA TAAATCTGTT 
TGGAACATAA TATGTAAAAA TGAGTATAGG AAGTGTGCAG TGGTCGAAAG CTATGAGAGC 
ATGAAGCATG TCATCAGAAA GATTCTCAAG GATGATTCTG ATGAGTTCCA TATTTTTATA 
GCTATTTTTG AAGAAATAGA TTTCGCGATA AGGAAGGATA GGTTCACCGA AACTTTCAAG 
CTTCCAGAGC TGATGGAAAT TCATGCCCGA GTTGTTGAAT TGATTAGTTT TCTTTTGACT 
CGTCCCGCTG AAAAGCACAA ACAGAAGGTA GTGAAAGACC TTCAGAACTT GTACGAAGGT 
CTGCTACACG ACTTTCCACT ACAGCCACAC ATCTTTCTGG AAAGTATCAA GGCACGCGCT 
TCATATCCCC AAAATAACAA AGGGACTGAG CTGTTCATGG ACGCTGTGGA ACTCCCAGAT 
AAAGGTGACG AGCACTTCTT TAAGAATTTA AAGAGGCTGC ATACCACTCT AAGCACCAGG 
GACCCTTTAC TGTATGTTCC GAAGGGACTA GAAGCAAGAC GACGAATCTC TTTTTTCAGT 
AACTCGTTGT TCATGACCAT GCCGCGTGCA CCACAGGTTG AAAGAATGCT GGCTTTCAGT 
GTTTTGACAC CTTACTACAA TGAGGAAGTT ATTTTCAGTA AGCATCAGTT GAAAGAGGAA 
AACGAGGATG GCGTGACCAT TCTGTTCTAC CTTCAACGGA TCTTTCCAGA GGACTGGTTA 
AATTTCCTGG AACGGATGAA GAAGCTCGAA CTTAATGAGA GTGAGTTATG GGAAAAGGAT 
GACGCTCTTG AACTTAGACT TTGGGCTTCT TTTAGAGGAC AGACACTTGC GCGAACTGTA 
CGAGGAATGA TGTATTATAA AAGGGCATTA GAAGTGCAGA CCTTTCTGGA CTCAGCAACT 
GAAGATGAGC TACTTGGCAT AAAGGAACTT CTAGAACGAG GCTCTAGCAC AAACAGCAGA 
GGTTCTATGC GCTCCATAGG TTCTATGGGT TCTATTGGTT CTGAGCTTGA AGTTGCAGAG 
TTAAATCGAC AACGCAAGTT AGAACAGGAT CTTGCAAATG CTGCAATGAA GTTTACATAC 
GTGGTAACCT GTCAAATATA CGGGGCTCAA AAGAAGGCCA ATGATGTTCG TGCAGCAGAT 
ATACTGCGAT TAATGAAGAC TCATACAGGA CTACGGATTG CCTATGTGGA TGAAAGATCT 
GAATCATATT TTGATGAAAA TATTGGAGAG TACGTAACGC GTCAACTATA TTATTCTGTT 
TTAGTGAAAT ATGATCCAGA TTTGAAACAG GAAGTCGAGA TTTATCGAAT TCGATTACCT 
GGTCCTCTGA AGCTTGGAGA AGGGAAGCCA GAAAATCAGA ATCATGCCCT TATATTTACA 
AGAGGTGATG CTGTTCAAAC TATAGACATG AATCAGGAAA TGTACTTCGA GGAGGCAATA 
AAGATGAGAA ATCTGCTCCA AGAATTTACA GTGTATCATG GAACCCGAAA ACCCACAATC 
TTGGGTGTGC GAGAGCATGT CTTCACTGGT TCAGTGTCAT CACTGGCCTG GTTCATGTCC 
GCTCAAGAGA CAGTGTTTGT CACCCTCAGC CAGCGTGTCC TCGCCAATCC TTTGAAGATT 
CGAATGCATT ATGGTCATCC TGATGTATTT GATCGTTTGT GGTTTCTGAC TCGCGGCGGG 
ATAAGCAAAG CATCCCGGAC TATTAATATT AGTGAAGATA TTTTTGCAGG ATTTAATTGT 
ACATTAAGAG GTGGTAATGT AACTCACCAT GAGTACATTC AGGCTGGCAA GGGAAGGGAT 
GTTGGGCTCA ACCAAATCGC TATGTTTGAG GCAAAGGTGG CCAGTGGGAA TGGTGAACAG 
ATTCTAAGTC GTGATGTGTA TCGCCTTGGT CACCATTTAG ATTTCTTTCG CATGCTCTCT 
TTCTATTATA CCACGGTGGG CTTCTTTGTG AGCAACATGA TGGTTGTACT CACTGTCTAC 
ACCTTTCTCT GGGGCCGTGT CTACTTGGCT TTAAGCGGCA TAGAGGAATC CTTAACATCT 
GGTTCACCAG CCCTTGAAAA TTCTGCTCTC ACAGCGACTT TGAACCAGCA GCTTGTGGTA 
CAGTTGGGGT TGCTGACTGC TCTACCCATG GTTGTGGAGG ATGCATTAGA GCACGGTTTC 
ACTACGGCTT TGTGGAATAT GATAACCATG CAGCTTCAAC TTGCTTCCAT CTTTTTCACC 
TTTTCAATGG GCACTCGTTG TCACTACTTC GGTCGGACGC TTCTTCACGG TGGTGCTAAG 
TATCGAGCAA CTGGTCGTGG CTTTGTAGTT AAGCATGAAA AGTTTGCTGA AAACTATCGA 
CTTTACTCCA GAAGCCATTT TGTTAAAGGA ATAGAGCTTC TCTTGCTGCT TCTGTGTTAC 
TTGGCGTATG GTGTATCAAG CTCATCTGGT ACATACATTT TGGTCAACAT CTCCAGCTGG 
TTTTTAGCTC TGACATGGGT AATGGGACCT TTTGTGTTTA ATCCGTCTGG ATTTGATTGG 
TTGAAAACTG TTGAAGATTT TGGGGACTTC ATGCAGTGGA TATGGTTCAA GGGTGATGTG 
TTCGTTAAAG TTGAGCAAAG TTGGGAGATA TGGTGGGAAG AGGAGCAAGC TCATTTACGA 
ACCACGGGAT TGTGGGGGAA GCTACTAGAG ATCGTACTTG ATTTGCGATT CTTCATTTTC 
CAGTATGGCA TCGTGTACCA TTTGGGTATC ACAGGGAATA ACACAAGCAT CTTCGTCTAC 
CTTGCATCAT GGAGCTACAT GCTTTTTGCA GCCCTTCTCC ACTTCATCCT TTCCAATGCT 
AATGAGAAGT TAGCTGCCAA CAACCATGGT CTGTACCGCG CTATTCAAGC CCTTGCGATC 
GCTATCATTA CTGCCTTAGT TGTCGTACTG TGGGTCGTAA CCAACTTCAC ATTTGTGGAC 
ATCATAGCAA GCTTTCTTGC ATTCTTACCG ACAGGCTGGG GAATCATTCA AATCTGCCTT 
GTTTTGAGGA GGCCATTTTT GGAAAACTCC CCTCTATGGA GTACCATTGT GGCCGTTGCG 
CGTCTATACG ATCTTGCCAT GGGCATTATT GTCATGGCTC CCGTTGCTGT GCTGTCGTGG 
TTGCCAGGAT TTCAAGCCAT GCAAACTAGA ATACTTTACA ACGAAGCCTT CAGCAGAGGT 
CTCCAAATCA GTAGGTTGTT GGCTGGCAAG AGGAACCGCA ATATTGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024373470.1
CDS	225..5075
Translation	MPECLCFIYHHMLGELNRLLEFSGADDVLAVMPTYTGVNGFLNHVVVPLYTILKLEADSNNNGTAPHSSWRNYDDLNEYFWTSRCFKQLQWPLQTKSSYLVEPRREKGYLSRGRKPQSEKVGKTGFVEQRSFWYIFRSFDKLWIGYLLMLQASVVLLWHNGGAPWIELQKPDPFARFMSIFISWALLRFLQGLLDVGSQYSLVSKDTKLIGVRMVLKLLVAATWAILFIIYYRRMWWQRNIDQYWTEIANQKLYEFLYIAAAFIVPEVLAILLFIVPWVRNFVETSTWKVFHLMTWWFQSRGYVGRGLREGIMDNVRYTLFWACVLTSKFAFSYWLQIRPLIAPTKQILEATDVRYKWHEFFPDGNRAAVVALWAPVLMIYFMDTQIWYSIWSSGIGAFVGLLQHLGEIRNVEQLQLRFQIFPSAFQFSLMPVDDSVTRTVWAGAKDLLKRLSLRYGWSSVYDKMEWGQIEGGRFAHVWNEIIKTFREEDLISDREVELMEIPQGAWRVSVFQWPSTLLANQILLALYSIRYHRGDDKSVWNIICKNEYRKCAVVESYESMKHVIRKILKDDSDEFHIFIAIFEEIDFAIRKDRFTETFKLPELMEIHARVVELISFLLTRPAEKHKQKVVKDLQNLYEGLLHDFPLQPHIFLESIKARASYPQNNKGTELFMDAVELPDKGDEHFFKNLKRLHTTLSTRDPLLYVPKGLEARRRISFFSNSLFMTMPRAPQVERMLAFSVLTPYYNEEVIFSKHQLKEENEDGVTILFYLQRIFPEDWLNFLERMKKLELNESELWEKDDALELRLWASFRGQTLARTVRGMMYYKRALEVQTFLDSATEDELLGIKELLERGSSTNSRGSMRSIGSMGSIGSELEVAELNRQRKLEQDLANAAMKFTYVVTCQIYGAQKKANDVRAADILRLMKTHTGLRIAYVDERSESYFDENIGEYVTRQLYYSVLVKYDPDLKQEVEIYRIRLPGPLKLGEGKPENQNHALIFTRGDAVQTIDMNQEMYFEEAIKMRNLLQEFTVYHGTRKPTILGVREHVFTGSVSSLAWFMSAQETVFVTLSQRVLANPLKIRMHYGHPDVFDRLWFLTRGGISKASRTINISEDIFAGFNCTLRGGNVTHHEYIQAGKGRDVGLNQIAMFEAKVASGNGEQILSRDVYRLGHHLDFFRMLSFYYTTVGFFVSNMMVVLTVYTFLWGRVYLALSGIEESLTSGSPALENSALTATLNQQLVVQLGLLTALPMVVEDALEHGFTTALWNMITMQLQLASIFFTFSMGTRCHYFGRTLLHGGAKYRATGRGFVVKHEKFAENYRLYSRSHFVKGIELLLLLLCYLAYGVSSSSGTYILVNISSWFLALTWVMGPFVFNPSGFDWLKTVEDFGDFMQWIWFKGDVFVKVEQSWEIWWEEEQAHLRTTGLWGKLLEIVLDLRFFIFQYGIVYHLGITGNNTSIFVYLASWSYMLFAALLHFILSNANEKLAANNHGLYRAIQALAIAIITALVVVLWVVTNFTFVDIIASFLAFLPTGWGIIQICLVLRRPFLENSPLWSTIVAVARLYDLAMGIIVMAPVAVLSWLPGFQAMQTRILYNEAFSRGLQISRLLAGKRNRNID

Target ORF information:

RefSeq Version	XM_024517702.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens callose synthase 11-like (LOC112281321), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024517702.1

ORF Insert Sequence:

ATGCCCGAGT GTTTGTGTTT CATCTATCAC CACATGCTCG GCGAACTGAA TAGGTTACTA 
GAATTTTCTG GAGCAGATGA CGTGCTTGCT GTGATGCCTA CCTACACGGG AGTTAATGGT 
TTTCTTAATC ATGTTGTTGT TCCTCTCTAT ACTATTTTGA AATTGGAGGC GGATTCTAAT 
AATAATGGGA CAGCCCCTCA CTCTTCATGG CGAAACTATG ACGATCTTAA TGAATATTTT 
TGGACTTCAC GTTGTTTCAA ACAGCTTCAA TGGCCTCTGC AAACTAAGAG TAGTTATCTT 
GTTGAGCCTA GGCGAGAGAA AGGTTACCTT AGCCGTGGGA GGAAACCGCA AAGTGAAAAG 
GTGGGGAAAA CTGGATTCGT TGAACAGCGG TCATTCTGGT ATATCTTTCG GAGCTTCGAT 
AAGTTGTGGA TAGGTTATCT TCTTATGCTG CAGGCTTCTG TTGTGTTGCT GTGGCACAAC 
GGTGGGGCTC CATGGATTGA ATTGCAAAAG CCAGATCCTT TCGCACGTTT CATGAGTATA 
TTTATCAGTT GGGCTCTTCT ACGCTTTTTA CAAGGATTGT TGGATGTGGG GAGTCAATAT 
AGCCTGGTTT CAAAAGATAC AAAACTTATT GGTGTTCGCA TGGTTCTCAA GCTTCTGGTA 
GCAGCTACAT GGGCCATCCT TTTCATCATC TATTACAGAA GGATGTGGTG GCAGCGGAAC 
ATCGATCAGT ACTGGACTGA AATTGCAAAC CAAAAGTTGT ATGAGTTCTT GTACATTGCT 
GCAGCCTTTA TTGTACCGGA GGTCCTAGCT ATTTTACTAT TTATTGTACC ATGGGTGCGT 
AATTTCGTTG AGACCTCAAC TTGGAAAGTA TTTCATCTCA TGACTTGGTG GTTTCAGTCA 
CGTGGTTATG TTGGTCGAGG TCTTCGTGAA GGTATCATGG ACAACGTGAG GTATACCCTA 
TTTTGGGCAT GCGTCCTTAC ATCCAAATTC GCTTTCAGCT ATTGGCTCCA GATTAGACCT 
TTAATTGCAC CAACAAAACA GATCCTTGAA GCCACCGACG TCAGATACAA GTGGCACGAG 
TTTTTTCCAG ATGGTAATCG AGCGGCTGTA GTTGCGCTGT GGGCTCCTGT TCTTATGATC 
TATTTCATGG ATACTCAAAT ATGGTATTCA ATCTGGTCCT CTGGGATTGG AGCTTTTGTT 
GGTCTCCTTC AACACCTTGG GGAGATTCGG AACGTGGAGC AGCTCCAACT AAGATTTCAG 
ATCTTTCCAA GTGCATTTCA ATTCAGTTTA ATGCCAGTAG ACGACTCTGT AACGAGGACT 
GTGTGGGCAG GTGCAAAAGA CTTGCTTAAG CGGCTTAGCT TGAGATATGG GTGGTCATCC 
GTTTATGACA AGATGGAATG GGGTCAAATT GAAGGTGGAC GTTTTGCCCA TGTTTGGAAC 
GAGATTATTA AAACCTTTCG CGAAGAGGAT CTCATAAGTG ACAGAGAGGT GGAGCTTATG 
GAAATTCCTC AAGGAGCATG GAGGGTCTCC GTTTTTCAAT GGCCTTCAAC CTTGTTGGCT 
AACCAAATTC TGCTTGCCCT ATACAGTATT CGTTACCATC GAGGAGACGA TAAATCTGTT 
TGGAACATAA TATGTAAAAA TGAGTATAGG AAGTGTGCAG TGGTCGAAAG CTATGAGAGC 
ATGAAGCATG TCATCAGAAA GATTCTCAAG GATGATTCTG ATGAGTTCCA TATTTTTATA 
GCTATTTTTG AAGAAATAGA TTTCGCGATA AGGAAGGATA GGTTCACCGA AACTTTCAAG 
CTTCCAGAGC TGATGGAAAT TCATGCCCGA GTTGTTGAAT TGATTAGTTT TCTTTTGACT 
CGTCCCGCTG AAAAGCACAA ACAGAAGGTA GTGAAAGACC TTCAGAACTT GTACGAAGGT 
CTGCTACACG ACTTTCCACT ACAGCCACAC ATCTTTCTGG AAAGTATCAA GGCACGCGCT 
TCATATCCCC AAAATAACAA AGGGACTGAG CTGTTCATGG ACGCTGTGGA ACTCCCAGAT 
AAAGGTGACG AGCACTTCTT TAAGAATTTA AAGAGGCTGC ATACCACTCT AAGCACCAGG 
GACCCTTTAC TGTATGTTCC GAAGGGACTA GAAGCAAGAC GACGAATCTC TTTTTTCAGT 
AACTCGTTGT TCATGACCAT GCCGCGTGCA CCACAGGTTG AAAGAATGCT GGCTTTCAGT 
GTTTTGACAC CTTACTACAA TGAGGAAGTT ATTTTCAGTA AGCATCAGTT GAAAGAGGAA 
AACGAGGATG GCGTGACCAT TCTGTTCTAC CTTCAACGGA TCTTTCCAGA GGACTGGTTA 
AATTTCCTGG AACGGATGAA GAAGCTCGAA CTTAATGAGA GTGAGTTATG GGAAAAGGAT 
GACGCTCTTG AACTTAGACT TTGGGCTTCT TTTAGAGGAC AGACACTTGC GCGAACTGTA 
CGAGGAATGA TGTATTATAA AAGGGCATTA GAAGTGCAGA CCTTTCTGGA CTCAGCAACT 
GAAGATGAGC TACTTGGCAT AAAGGAACTT CTAGAACGAG GCTCTAGCAC AAACAGCAGA 
GGTTCTATGC GCTCCATAGG TTCTATGGGT TCTATTGGTT CTGAGCTTGA AGTTGCAGAG 
TTAAATCGAC AACGCAAGTT AGAACAGGAT CTTGCAAATG CTGCAATGAA GTTTACATAC 
GTGGTAACCT GTCAAATATA CGGGGCTCAA AAGAAGGCCA ATGATGTTCG TGCAGCAGAT 
ATACTGCGAT TAATGAAGAC TCATACAGGA CTACGGATTG CCTATGTGGA TGAAAGATCT 
GAATCATATT TTGATGAAAA TATTGGAGAG TACGTAACGC GTCAACTATA TTATTCTGTT 
TTAGTGAAAT ATGATCCAGA TTTGAAACAG GAAGTCGAGA TTTATCGAAT TCGATTACCT 
GGTCCTCTGA AGCTTGGAGA AGGGAAGCCA GAAAATCAGA ATCATGCCCT TATATTTACA 
AGAGGTGATG CTGTTCAAAC TATAGACATG AATCAGGAAA TGTACTTCGA GGAGGCAATA 
AAGATGAGAA ATCTGCTCCA AGAATTTACA GTGTATCATG GAACCCGAAA ACCCACAATC 
TTGGGTGTGC GAGAGCATGT CTTCACTGGT TCAGTGTCAT CACTGGCCTG GTTCATGTCC 
GCTCAAGAGA CAGTGTTTGT CACCCTCAGC CAGCGTGTCC TCGCCAATCC TTTGAAGATT 
CGAATGCATT ATGGTCATCC TGATGTATTT GATCGTTTGT GGTTTCTGAC TCGCGGCGGG 
ATAAGCAAAG CATCCCGGAC TATTAATATT AGTGAAGATA TTTTTGCAGG ATTTAATTGT 
ACATTAAGAG GTGGTAATGT AACTCACCAT GAGTACATTC AGGCTGGCAA GGGAAGGGAT 
GTTGGGCTCA ACCAAATCGC TATGTTTGAG GCAAAGGTGG CCAGTGGGAA TGGTGAACAG 
ATTCTAAGTC GTGATGTGTA TCGCCTTGGT CACCATTTAG ATTTCTTTCG CATGCTCTCT 
TTCTATTATA CCACGGTGGG CTTCTTTGTG AGCAACATGA TGGTTGTACT CACTGTCTAC 
ACCTTTCTCT GGGGCCGTGT CTACTTGGCT TTAAGCGGCA TAGAGGAATC CTTAACATCT 
GGTTCACCAG CCCTTGAAAA TTCTGCTCTC ACAGCGACTT TGAACCAGCA GCTTGTGGTA 
CAGTTGGGGT TGCTGACTGC TCTACCCATG GTTGTGGAGG ATGCATTAGA GCACGGTTTC 
ACTACGGCTT TGTGGAATAT GATAACCATG CAGCTTCAAC TTGCTTCCAT CTTTTTCACC 
TTTTCAATGG GCACTCGTTG TCACTACTTC GGTCGGACGC TTCTTCACGG TGGTGCTAAG 
TATCGAGCAA CTGGTCGTGG CTTTGTAGTT AAGCATGAAA AGTTTGCTGA AAACTATCGA 
CTTTACTCCA GAAGCCATTT TGTTAAAGGA ATAGAGCTTC TCTTGCTGCT TCTGTGTTAC 
TTGGCGTATG GTGTATCAAG CTCATCTGGT ACATACATTT TGGTCAACAT CTCCAGCTGG 
TTTTTAGCTC TGACATGGGT AATGGGACCT TTTGTGTTTA ATCCGTCTGG ATTTGATTGG 
TTGAAAACTG TTGAAGATTT TGGGGACTTC ATGCAGTGGA TATGGTTCAA GGGTGATGTG 
TTCGTTAAAG TTGAGCAAAG TTGGGAGATA TGGTGGGAAG AGGAGCAAGC TCATTTACGA 
ACCACGGGAT TGTGGGGGAA GCTACTAGAG ATCGTACTTG ATTTGCGATT CTTCATTTTC 
CAGTATGGCA TCGTGTACCA TTTGGGTATC ACAGGGAATA ACACAAGCAT CTTCGTCTAC 
CTTGCATCAT GGAGCTACAT GCTTTTTGCA GCCCTTCTCC ACTTCATCCT TTCCAATGCT 
AATGAGAAGT TAGCTGCCAA CAACCATGGT CTGTACCGCG CTATTCAAGC CCTTGCGATC 
GCTATCATTA CTGCCTTAGT TGTCGTACTG TGGGTCGTAA CCAACTTCAC ATTTGTGGAC 
ATCATAGCAA GCTTTCTTGC ATTCTTACCG ACAGGCTGGG GAATCATTCA AATCTGCCTT 
GTTTTGAGGA GGCCATTTTT GGAAAACTCC CCTCTATGGA GTACCATTGT GGCCGTTGCG 
CGTCTATACG ATCTTGCCAT GGGCATTATT GTCATGGCTC CCGTTGCTGT GCTGTCGTGG 
TTGCCAGGAT TTCAAGCCAT GCAAACTAGA ATACTTTACA ACGAAGCCTT CAGCAGAGGT 
CTCCAAATCA GTAGGTTGTT GGCTGGCAAG AGGAACCGCA ATATTGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

REQUEST A QUOTE

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[email protected]

PHONE

1-877-436-7274

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FAX

1-732-210-0262

LOC112281321 cDNA ORF clone,

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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REQUEST A QUOTE

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PHONE

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ONLINE FORM

FAX

Gene Symbol	LOC112281321
Entrez Gene ID	112281321