CAST cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAST
Entrez Gene ID	100174647
Full Name	calpastatin
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001134097.1	NP_001127569.1	calpastatin

Pathways from BioSystems
Gene Ontology

The following CAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSa19862	NM_001134097.1 Latest version!	Pongo abelii calpastatin (CAST), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAST ( NM_001134097.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> NP_001127569.1 Pongo abelii calpastatin (CAST), mRNA.

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CloneID	OSa19862
Clone ID Related Accession (Same CDS sequence)	NM_001134097.1
Accession Version	NM_001134097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Pongo abelii(Sumatran orangutan)
Product	calpastatin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR860493.1. ##Evidence-Data-START## Transcript exon combination :: CR860493.1 [ECO:0000332] ##Evidence-Data-END##

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGACGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA AGGATGCTCT CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA 
CCGCAAGACA CAATTTCTGC TGGTGGAGAG AGTGTTGCTG GTGTTGCTGC AATATCTGGC 
AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT 
AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT 
ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGT ACCAGAAGTT 
TCAGATCCAA TGAGTTCTAC CTACATAGAG GAATTGGGTG AAAGAGAAGT CACAATTCCT 
CCAAAATATA GGGAACTATT GGCTAAAAAC GAAGGGATCA CAGGGCCTCC TCCAGACTCC 
TCGAAACCTA TGGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG 
TCGCCTACAG CTGCTGGAAA GAAGACTGAA AAAGAGGAAT CTATAGAAGT TTTGAAAGCT 
CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG 
AAGGATACAA TGAGCGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA 
GCAGAACCTG AGCTCGACCT CAGCTCGATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA 
GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA 
CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT 
CGGAGTGAAT CAGAACTCAT TGATGAACTT TCGGAAGATT TTGACCGGTC TGAATGTAAA 
GAAAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCGA AGGCCGCTGC TCCAGCTCCT 
GTGGCGGAGG CTGTGTCTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG 
GCTACCTTGA AGGGCACGGT GCCAGACGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG 
AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC 
AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA 
TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG 
CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA 
CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG 
GTTTCCCAAA CCCCAGCTTC AACGACCCAC GCTGGAGCCC CACCCCGTGA TACTTCGCAG 
AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ATAGTCTAGG ACAAAGGCAG 
CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA 
CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG 
GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG 
AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC 
TCCACTACAG AAACCTCACA GAACACAGCA AAGGGTAAGG GCAAGAAGAC TGCTTCCAGC 
TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT 
TCCAAGCCAA AAGATGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127569.1
CDS	137..2197
Translation	MNPTETKAVKTEPEKTSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSKDALNKKAVSRSAEQQPSEKSTEPKTKPQDTISAGGESVAGVAAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTVPEVSDPMSSTYIEELGEREVTIPPKYRELLAKNEGITGPPPDSSKPMGPDDAIDALSSDFTCGSPTAAGKKTEKEESIEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLSSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVAEAVSRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTHAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKGKGKKTASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001134097.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii calpastatin (CAST), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134097.1

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGACGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA AGGATGCTCT CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA 
CCGCAAGACA CAATTTCTGC TGGTGGAGAG AGTGTTGCTG GTGTTGCTGC AATATCTGGC 
AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT 
AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT 
ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGT ACCAGAAGTT 
TCAGATCCAA TGAGTTCTAC CTACATAGAG GAATTGGGTG AAAGAGAAGT CACAATTCCT 
CCAAAATATA GGGAACTATT GGCTAAAAAC GAAGGGATCA CAGGGCCTCC TCCAGACTCC 
TCGAAACCTA TGGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG 
TCGCCTACAG CTGCTGGAAA GAAGACTGAA AAAGAGGAAT CTATAGAAGT TTTGAAAGCT 
CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG 
AAGGATACAA TGAGCGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA 
GCAGAACCTG AGCTCGACCT CAGCTCGATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA 
GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA 
CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT 
CGGAGTGAAT CAGAACTCAT TGATGAACTT TCGGAAGATT TTGACCGGTC TGAATGTAAA 
GAAAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCGA AGGCCGCTGC TCCAGCTCCT 
GTGGCGGAGG CTGTGTCTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG 
GCTACCTTGA AGGGCACGGT GCCAGACGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG 
AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC 
AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA 
TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG 
CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA 
CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG 
GTTTCCCAAA CCCCAGCTTC AACGACCCAC GCTGGAGCCC CACCCCGTGA TACTTCGCAG 
AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ATAGTCTAGG ACAAAGGCAG 
CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA 
CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG 
GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG 
AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC 
TCCACTACAG AAACCTCACA GAACACAGCA AAGGGTAAGG GCAAGAAGAC TGCTTCCAGC 
TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT 
TCCAAGCCAA AAGATGATTA A