LOC7488593 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC7488593
Entrez Gene ID	7488593
Full Name	hypothetical protein
Synonyms	POPTRDRAFT_756182
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024595350.1	XP_024451118.1	stromal processing peptidase, chloroplastic isoform X1
XM_024595352.1	XP_024451120.1	stromal processing peptidase, chloroplastic isoform X2
XM_024595353.1	XP_024451121.1	stromal processing peptidase, chloroplastic isoform X3
XM_002301712.3	XP_002301748.3	stromal processing peptidase, chloroplastic isoform X4
XM_024595351.1	XP_024451119.1	stromal processing peptidase, chloroplastic isoform X1
XM_002301712.2	XP_002301748.2	hypothetical protein
XM_024595354.1	XP_024451122.1	stromal processing peptidase, chloroplastic isoform X5

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, D?jardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasj?rvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Lepl? JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouz? P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC7488593 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC7488593 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa424732	XM_024595351.1 Latest version!	Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa424732	XM_024595350.1 Latest version!	Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa424733	XM_024595352.1 Latest version!	Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa424734	XM_024595353.1 Latest version!	Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa424735	XM_002301712.3 Latest version!	Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa424736	XM_024595354.1 Latest version!	Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPa06350	XM_002301712.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0002s23680g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC7488593 ( XM_024595350.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451118.1 Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa424732
Clone ID Related Accession (Same CDS sequence)	XM_024595350.1 , XM_024595351.1
Accession Version	XM_024595350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	stromal processing peptidase, chloroplastic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGTA CAGCTGCCCC AAGCTCTGTA TTGATGACGA GCTTGCCTCA AATCCGTACA 
GATGATACTT TCTCAAGGAA GAACCGGATA AACCTAATTC GTTCCCCTTT GATTCGATTC 
CAAGCCAGCC ATCATCAATC TTGTCATTGC ATTTCCTCCA AAAGGTGGAA ACATGAGTTT 
GCTGCCGGTA GATCAGGTAC TAGTAGAAAG AAGAATAATG CTTGGAAACA TTGTAGTTCG 
TTCCTTGGTG AGAGAGTGGT TGAGGCATCT TTTCCAGAAC AATTTAAGTG CATGTCTTGC 
TCCCTTAACC GCCGTAGAAG CCGCTATAGC ATCAAGAGAT CTATACCTAG AGCCTTTATT 
GATAAGTCTG CTTTTCGTTT GTCTGGCCAT TCATTCGATA CTGCTTCTGC AAAGCATGTT 
CATGTCCCAT GTGCATCAAT GGGTCCAAAT GAGCCACATG CAGCAAGTAT AGCCTGCCCA 
GATGGTATTC TTGAGAGACA AGATTCCCAT TTATTAGATT CTGAATTAGA AAGAGCAAGA 
CTACTTGAGT TTTTGAATTC TGAACTTCCA TGTCACCCCA AGTTACACAG AGGACAACTG 
AAAAATGGAC TCTGTTATCT GATTCTACCA AATAAAGTTC CACCAAACAG CTTTCAATTT 
TTTAGGTTTG AGGCACACAT GGAAGTTCAT GTAGGATCAA TAGACGAGGA AGATGATGAG 
CAAGGAATTG CACATATGAT TGAACATGTT GCATTCCTTG GAAGTAAGAA ACGTGAGAAA 
CTTCTTGGAA CAGGGGCCCG ATCTAATGCT TACACTGATT TCCACCATAC AGTGTTCCAC 
ATTCATTCAC CAACTTGTAC AAAGGATGCT GATGGAGATC TACTCCCCTC TGTGTTGGAT 
GCCTTGAATG AGATAGCTTT CCACCCGAGC TTCCTCGCTT CTCGAGTTGA AAAGGAAAGG 
CGTGCTATAC TTTCAGAACT ACAGATGATG AATACTATAG AATATCGTGT TGACTGCCAG 
TTGTTACAAC ATCTACATTC AGAAAACAAG CTGAGCAAAA GGTTCCCAAT AGGATTGGAA 
GAGCAGATTA AGAAGTGGGA TGCAGAGAAA ATTAGGAAAT TCCATGAGCG TTGGTACTTC 
CCAGCAAATG CAACTCTATA CATTGTTGGT GACATTGATA ACATTTCAAA GACGGTTCAC 
CAAATTGAAA ATGTCTTTGG ACAAACTGGC CTGGAAAACA AGACAGTTTC TGCTCCTTCT 
CCAAGTGCAT TTGGTGCAAT GGCTAGTTTT CTTGCTCCTA AGGTCTCAGT TGGGCTCCCT 
GGAAGTTCAT CTCGTGAAAA GTCATCTAGT TCTCTAGACC AATCCAAAAT CATTAAAAGG 
GAAAGGCATG CAGTCCGTCC TCCTGTCGAG CATTATTGGT CTCTTCCTGG AAGCAATGCA 
AATTTGAAGC CACCACAGAT ATTTCAGCAT GAGTTTCTTC AGAATTTCTC AATTAATATG 
TTCTGCAAGA TTCCGGTGAG CAAGGTCCAA ACGAATGGTG ACTTGTGCAG TGTTTTGATG 
AAGAGAATAT TTCTATCTGC ACTGCACTTC CGAATTAATA CAAGATACAA GAGTTCAAAT 
CCACCATTCA CTTCAGTTGA ATTGGATCAT AGTGATTCTG GAAGGGAAGG ATGCACTGTC 
ACAACTCTTA CAGTGACTGC AGAACCCAAG AATTGGCAAA ATGCAATTAA AGTTGCTGTT 
CAGGAGGTTC GAAGGCTTAA AGAATTTGGT GTCACAAAGG GTGAATTAAC TCGCTATATG 
GATGCACTTT TAAAAGATAG CGAACATCTG GCAGCTATGA TTGATAATGT ATCATCTGTT 
GATAATTTGG AATTTATCAT GGAGAGTGAT GCTCTTGGCC ATACTGTGAT GGATCAGAGA 
CAAGGACATG AGAGTTTGTT TGCTGTTGCT GGAATGGTCA CACTTGAAGA AGTCAATTCT 
ATTGGTGCCA AGTTATTAGA ATTTATCTCT GATTTTGGAA AACCTACTGC GCCCATTCCT 
GCAGCAATCG TTGCATGTGT TCCTACAAAA GTGCATATTG ATGGATTGGG TGAAACCGAG 
TTCAAGATAT CCTCAAGTGA GATTACAGCT GCCATAAAAT CAGGATTGGA GGAAGCAATT 
GAGGCTGAGC CAGAGCTTGA AGTGCCAAAA GAATTGATAT CTTCAACACA GTTAGAGGAG 
TTAAGGTTAG AACGTAGGCC ATCCTTTGTT CCCTTACTTC CAGATGCAGG CTATACGAAA 
TTGCATGACC AAGAAACAGG CATCACTCAG TGTCGTCTTT CAAATGGAAT TGCTGTAAAT 
TACAAGATAT CCAAAAGTGA ATCCCGGGGA GGTGTCATGC GGCTTATTGT GGGTGGGGGC 
CGAGCAGCTG AAAGTTCCGA GTCTAAAGGA GCTGTTGTTG TGGGTGTTCG AACTCTCAGT 
GAGGGTGGTC GTGTTGGCAG CTTTTCGAGA GAGCAGGTAG AACTTTTTTG TGTGAATCAC 
CTAATAAATT GCTCTTTGGA GTCAACTGAG GAATTTATTT GTATGGAGTT CCGCTTCACT 
CTGCGAGACA ATGGGATGCA AGCAGCATTT GAATTACTTC ATATGGTACT TGAGAATAGT 
GTCTGGCTGG ATGATGCATT TGACAGAGCA AGGCAACTCT ACTTGTCATA TTACCGGTCC 
ATTCCCAAAA GCTTAGAACG GGCAACTGCT CACAAGCTCA TGACAGCAAT GTTGAATGGG 
GATGAGCGGT TCATTGAGCC AACCCCACAA TCATTACAAA ATTTAACACT GAAATCCGTA 
AAAGATGCAG TGATGAATCA GTTTGTTGGT GGAAACATGG AGGTAAGTAT TGTTGGAGAT 
TTCTCAGAGG AGGAGGTTCA GTCTTGCATT ATTGATTACC TGGGCACAGT TAGAGCAACA 
AGAGATTCTG ATCAGGAACA AGAATTTAAT CCTGTCATGT TTCGACCATC CCCTTCTGAT 
TTGCAGTTTC AACAAGTGTT TTTGAAGGAT ACTGATGAGA GAGCATGTGC ATATATTGCT 
GGTCCTGCAC CAAATCGTTG GGGTTTCACT GTTGATGGAA CAGACCTGTT CAAGTCAATG 
AGTGGCTTTT CGGTTTCAGC TGCTGGCCAA TGCTTTGCAG ATGCACAACC AATATCTGAA 
ACACAACAGA TAGACGGGAT GGATGTTCAG AAGGATATGC AAGGAAAACT TCGCTGTCAT 
CCACTCTTCT TTGGCATAAC AATGGGGCTA CTGGCTGAGA TCATAAATTC TCGGCTTTTT 
ACCACAGTTA GGGATTCTCT TGGGTTGACA TATGATGTAT CGTTTGAGTT AAGCCTATTT 
GATAGGCTTA AGCTTGGTTG GTATGTTGTA TCAGTAACAT CAACTCCAGG CAAGGTACAT 
AAAGCGGTTG ATGCATGCAA AAGTGTTCTT AGAGGGTTGC ATAGCAACAA GGTTGCCCAG 
AGAGAGTTGG ACAGGGCAAG ACGGACGCTG CTAATGAGAC ATGAAGCTGA GATTAAGTCC 
AATGCCTATT GGCTTGGATT GCTTGCTCAT TTGCAAGCCT CTTCTGTTCC GAGAAAGGAC 
GTCTCATGTA TTAAAGATCT CACTTCACTC TATGAAGCTG CCACAATTGA GGACATATAC 
CTTGCATATG AACAATTGAA AGTAGATGAG GATTCTCTGT ATTCATGCAT TGGGGTTGCT 
GGGACACAAG CTGGAGAGGA GATTAATGCT CCATTAGAAG TGGAAGAAAC AGATGATGGT 
TTGCAGGGAG GCATACCTGT GGGACGTGGT TTGTCTACAA TGACACGACC AACAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451118.1
CDS	212..4051
Translation	MASTAAPSSVLMTSLPQIRTDDTFSRKNRINLIRSPLIRFQASHHQSCHCISSKRWKHEFAAGRSGTSRKKNNAWKHCSSFLGERVVEASFPEQFKCMSCSLNRRRSRYSIKRSIPRAFIDKSAFRLSGHSFDTASAKHVHVPCASMGPNEPHAASIACPDGILERQDSHLLDSELERARLLEFLNSELPCHPKLHRGQLKNGLCYLILPNKVPPNSFQFFRFEAHMEVHVGSIDEEDDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTCTKDADGDLLPSVLDALNEIAFHPSFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKWDAEKIRKFHERWYFPANATLYIVGDIDNISKTVHQIENVFGQTGLENKTVSAPSPSAFGAMASFLAPKVSVGLPGSSSREKSSSSLDQSKIIKRERHAVRPPVEHYWSLPGSNANLKPPQIFQHEFLQNFSINMFCKIPVSKVQTNGDLCSVLMKRIFLSALHFRINTRYKSSNPPFTSVELDHSDSGREGCTVTTLTVTAEPKNWQNAIKVAVQEVRRLKEFGVTKGELTRYMDALLKDSEHLAAMIDNVSSVDNLEFIMESDALGHTVMDQRQGHESLFAVAGMVTLEEVNSIGAKLLEFISDFGKPTAPIPAAIVACVPTKVHIDGLGETEFKISSSEITAAIKSGLEEAIEAEPELEVPKELISSTQLEELRLERRPSFVPLLPDAGYTKLHDQETGITQCRLSNGIAVNYKISKSESRGGVMRLIVGGGRAAESSESKGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINCSLESTEEFICMEFRFTLRDNGMQAAFELLHMVLENSVWLDDAFDRARQLYLSYYRSIPKSLERATAHKLMTAMLNGDERFIEPTPQSLQNLTLKSVKDAVMNQFVGGNMEVSIVGDFSEEEVQSCIIDYLGTVRATRDSDQEQEFNPVMFRPSPSDLQFQQVFLKDTDERACAYIAGPAPNRWGFTVDGTDLFKSMSGFSVSAAGQCFADAQPISETQQIDGMDVQKDMQGKLRCHPLFFGITMGLLAEIINSRLFTTVRDSLGLTYDVSFELSLFDRLKLGWYVVSVTSTPGKVHKAVDACKSVLRGLHSNKVAQRELDRARRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDVSCIKDLTSLYEAATIEDIYLAYEQLKVDEDSLYSCIGVAGTQAGEEINAPLEVEETDDGLQGGIPVGRGLSTMTRPTT

Target ORF information:

RefSeq Version	XM_024595350.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595350.1

ORF Insert Sequence:

ATGGCGAGTA CAGCTGCCCC AAGCTCTGTA TTGATGACGA GCTTGCCTCA AATCCGTACA 
GATGATACTT TCTCAAGGAA GAACCGGATA AACCTAATTC GTTCCCCTTT GATTCGATTC 
CAAGCCAGCC ATCATCAATC TTGTCATTGC ATTTCCTCCA AAAGGTGGAA ACATGAGTTT 
GCTGCCGGTA GATCAGGTAC TAGTAGAAAG AAGAATAATG CTTGGAAACA TTGTAGTTCG 
TTCCTTGGTG AGAGAGTGGT TGAGGCATCT TTTCCAGAAC AATTTAAGTG CATGTCTTGC 
TCCCTTAACC GCCGTAGAAG CCGCTATAGC ATCAAGAGAT CTATACCTAG AGCCTTTATT 
GATAAGTCTG CTTTTCGTTT GTCTGGCCAT TCATTCGATA CTGCTTCTGC AAAGCATGTT 
CATGTCCCAT GTGCATCAAT GGGTCCAAAT GAGCCACATG CAGCAAGTAT AGCCTGCCCA 
GATGGTATTC TTGAGAGACA AGATTCCCAT TTATTAGATT CTGAATTAGA AAGAGCAAGA 
CTACTTGAGT TTTTGAATTC TGAACTTCCA TGTCACCCCA AGTTACACAG AGGACAACTG 
AAAAATGGAC TCTGTTATCT GATTCTACCA AATAAAGTTC CACCAAACAG CTTTCAATTT 
TTTAGGTTTG AGGCACACAT GGAAGTTCAT GTAGGATCAA TAGACGAGGA AGATGATGAG 
CAAGGAATTG CACATATGAT TGAACATGTT GCATTCCTTG GAAGTAAGAA ACGTGAGAAA 
CTTCTTGGAA CAGGGGCCCG ATCTAATGCT TACACTGATT TCCACCATAC AGTGTTCCAC 
ATTCATTCAC CAACTTGTAC AAAGGATGCT GATGGAGATC TACTCCCCTC TGTGTTGGAT 
GCCTTGAATG AGATAGCTTT CCACCCGAGC TTCCTCGCTT CTCGAGTTGA AAAGGAAAGG 
CGTGCTATAC TTTCAGAACT ACAGATGATG AATACTATAG AATATCGTGT TGACTGCCAG 
TTGTTACAAC ATCTACATTC AGAAAACAAG CTGAGCAAAA GGTTCCCAAT AGGATTGGAA 
GAGCAGATTA AGAAGTGGGA TGCAGAGAAA ATTAGGAAAT TCCATGAGCG TTGGTACTTC 
CCAGCAAATG CAACTCTATA CATTGTTGGT GACATTGATA ACATTTCAAA GACGGTTCAC 
CAAATTGAAA ATGTCTTTGG ACAAACTGGC CTGGAAAACA AGACAGTTTC TGCTCCTTCT 
CCAAGTGCAT TTGGTGCAAT GGCTAGTTTT CTTGCTCCTA AGGTCTCAGT TGGGCTCCCT 
GGAAGTTCAT CTCGTGAAAA GTCATCTAGT TCTCTAGACC AATCCAAAAT CATTAAAAGG 
GAAAGGCATG CAGTCCGTCC TCCTGTCGAG CATTATTGGT CTCTTCCTGG AAGCAATGCA 
AATTTGAAGC CACCACAGAT ATTTCAGCAT GAGTTTCTTC AGAATTTCTC AATTAATATG 
TTCTGCAAGA TTCCGGTGAG CAAGGTCCAA ACGAATGGTG ACTTGTGCAG TGTTTTGATG 
AAGAGAATAT TTCTATCTGC ACTGCACTTC CGAATTAATA CAAGATACAA GAGTTCAAAT 
CCACCATTCA CTTCAGTTGA ATTGGATCAT AGTGATTCTG GAAGGGAAGG ATGCACTGTC 
ACAACTCTTA CAGTGACTGC AGAACCCAAG AATTGGCAAA ATGCAATTAA AGTTGCTGTT 
CAGGAGGTTC GAAGGCTTAA AGAATTTGGT GTCACAAAGG GTGAATTAAC TCGCTATATG 
GATGCACTTT TAAAAGATAG CGAACATCTG GCAGCTATGA TTGATAATGT ATCATCTGTT 
GATAATTTGG AATTTATCAT GGAGAGTGAT GCTCTTGGCC ATACTGTGAT GGATCAGAGA 
CAAGGACATG AGAGTTTGTT TGCTGTTGCT GGAATGGTCA CACTTGAAGA AGTCAATTCT 
ATTGGTGCCA AGTTATTAGA ATTTATCTCT GATTTTGGAA AACCTACTGC GCCCATTCCT 
GCAGCAATCG TTGCATGTGT TCCTACAAAA GTGCATATTG ATGGATTGGG TGAAACCGAG 
TTCAAGATAT CCTCAAGTGA GATTACAGCT GCCATAAAAT CAGGATTGGA GGAAGCAATT 
GAGGCTGAGC CAGAGCTTGA AGTGCCAAAA GAATTGATAT CTTCAACACA GTTAGAGGAG 
TTAAGGTTAG AACGTAGGCC ATCCTTTGTT CCCTTACTTC CAGATGCAGG CTATACGAAA 
TTGCATGACC AAGAAACAGG CATCACTCAG TGTCGTCTTT CAAATGGAAT TGCTGTAAAT 
TACAAGATAT CCAAAAGTGA ATCCCGGGGA GGTGTCATGC GGCTTATTGT GGGTGGGGGC 
CGAGCAGCTG AAAGTTCCGA GTCTAAAGGA GCTGTTGTTG TGGGTGTTCG AACTCTCAGT 
GAGGGTGGTC GTGTTGGCAG CTTTTCGAGA GAGCAGGTAG AACTTTTTTG TGTGAATCAC 
CTAATAAATT GCTCTTTGGA GTCAACTGAG GAATTTATTT GTATGGAGTT CCGCTTCACT 
CTGCGAGACA ATGGGATGCA AGCAGCATTT GAATTACTTC ATATGGTACT TGAGAATAGT 
GTCTGGCTGG ATGATGCATT TGACAGAGCA AGGCAACTCT ACTTGTCATA TTACCGGTCC 
ATTCCCAAAA GCTTAGAACG GGCAACTGCT CACAAGCTCA TGACAGCAAT GTTGAATGGG 
GATGAGCGGT TCATTGAGCC AACCCCACAA TCATTACAAA ATTTAACACT GAAATCCGTA 
AAAGATGCAG TGATGAATCA GTTTGTTGGT GGAAACATGG AGGTAAGTAT TGTTGGAGAT 
TTCTCAGAGG AGGAGGTTCA GTCTTGCATT ATTGATTACC TGGGCACAGT TAGAGCAACA 
AGAGATTCTG ATCAGGAACA AGAATTTAAT CCTGTCATGT TTCGACCATC CCCTTCTGAT 
TTGCAGTTTC AACAAGTGTT TTTGAAGGAT ACTGATGAGA GAGCATGTGC ATATATTGCT 
GGTCCTGCAC CAAATCGTTG GGGTTTCACT GTTGATGGAA CAGACCTGTT CAAGTCAATG 
AGTGGCTTTT CGGTTTCAGC TGCTGGCCAA TGCTTTGCAG ATGCACAACC AATATCTGAA 
ACACAACAGA TAGACGGGAT GGATGTTCAG AAGGATATGC AAGGAAAACT TCGCTGTCAT 
CCACTCTTCT TTGGCATAAC AATGGGGCTA CTGGCTGAGA TCATAAATTC TCGGCTTTTT 
ACCACAGTTA GGGATTCTCT TGGGTTGACA TATGATGTAT CGTTTGAGTT AAGCCTATTT 
GATAGGCTTA AGCTTGGTTG GTATGTTGTA TCAGTAACAT CAACTCCAGG CAAGGTACAT 
AAAGCGGTTG ATGCATGCAA AAGTGTTCTT AGAGGGTTGC ATAGCAACAA GGTTGCCCAG 
AGAGAGTTGG ACAGGGCAAG ACGGACGCTG CTAATGAGAC ATGAAGCTGA GATTAAGTCC 
AATGCCTATT GGCTTGGATT GCTTGCTCAT TTGCAAGCCT CTTCTGTTCC GAGAAAGGAC 
GTCTCATGTA TTAAAGATCT CACTTCACTC TATGAAGCTG CCACAATTGA GGACATATAC 
CTTGCATATG AACAATTGAA AGTAGATGAG GATTCTCTGT ATTCATGCAT TGGGGTTGCT 
GGGACACAAG CTGGAGAGGA GATTAATGCT CCATTAGAAG TGGAAGAAAC AGATGATGGT 
TTGCAGGGAG GCATACCTGT GGGACGTGGT TTGTCTACAA TGACACGACC AACAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7488593 ( XM_024595351.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451119.1 Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa424732
Clone ID Related Accession (Same CDS sequence)	XM_024595350.1 , XM_024595351.1
Accession Version	XM_024595351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	stromal processing peptidase, chloroplastic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGTA CAGCTGCCCC AAGCTCTGTA TTGATGACGA GCTTGCCTCA AATCCGTACA 
GATGATACTT TCTCAAGGAA GAACCGGATA AACCTAATTC GTTCCCCTTT GATTCGATTC 
CAAGCCAGCC ATCATCAATC TTGTCATTGC ATTTCCTCCA AAAGGTGGAA ACATGAGTTT 
GCTGCCGGTA GATCAGGTAC TAGTAGAAAG AAGAATAATG CTTGGAAACA TTGTAGTTCG 
TTCCTTGGTG AGAGAGTGGT TGAGGCATCT TTTCCAGAAC AATTTAAGTG CATGTCTTGC 
TCCCTTAACC GCCGTAGAAG CCGCTATAGC ATCAAGAGAT CTATACCTAG AGCCTTTATT 
GATAAGTCTG CTTTTCGTTT GTCTGGCCAT TCATTCGATA CTGCTTCTGC AAAGCATGTT 
CATGTCCCAT GTGCATCAAT GGGTCCAAAT GAGCCACATG CAGCAAGTAT AGCCTGCCCA 
GATGGTATTC TTGAGAGACA AGATTCCCAT TTATTAGATT CTGAATTAGA AAGAGCAAGA 
CTACTTGAGT TTTTGAATTC TGAACTTCCA TGTCACCCCA AGTTACACAG AGGACAACTG 
AAAAATGGAC TCTGTTATCT GATTCTACCA AATAAAGTTC CACCAAACAG CTTTCAATTT 
TTTAGGTTTG AGGCACACAT GGAAGTTCAT GTAGGATCAA TAGACGAGGA AGATGATGAG 
CAAGGAATTG CACATATGAT TGAACATGTT GCATTCCTTG GAAGTAAGAA ACGTGAGAAA 
CTTCTTGGAA CAGGGGCCCG ATCTAATGCT TACACTGATT TCCACCATAC AGTGTTCCAC 
ATTCATTCAC CAACTTGTAC AAAGGATGCT GATGGAGATC TACTCCCCTC TGTGTTGGAT 
GCCTTGAATG AGATAGCTTT CCACCCGAGC TTCCTCGCTT CTCGAGTTGA AAAGGAAAGG 
CGTGCTATAC TTTCAGAACT ACAGATGATG AATACTATAG AATATCGTGT TGACTGCCAG 
TTGTTACAAC ATCTACATTC AGAAAACAAG CTGAGCAAAA GGTTCCCAAT AGGATTGGAA 
GAGCAGATTA AGAAGTGGGA TGCAGAGAAA ATTAGGAAAT TCCATGAGCG TTGGTACTTC 
CCAGCAAATG CAACTCTATA CATTGTTGGT GACATTGATA ACATTTCAAA GACGGTTCAC 
CAAATTGAAA ATGTCTTTGG ACAAACTGGC CTGGAAAACA AGACAGTTTC TGCTCCTTCT 
CCAAGTGCAT TTGGTGCAAT GGCTAGTTTT CTTGCTCCTA AGGTCTCAGT TGGGCTCCCT 
GGAAGTTCAT CTCGTGAAAA GTCATCTAGT TCTCTAGACC AATCCAAAAT CATTAAAAGG 
GAAAGGCATG CAGTCCGTCC TCCTGTCGAG CATTATTGGT CTCTTCCTGG AAGCAATGCA 
AATTTGAAGC CACCACAGAT ATTTCAGCAT GAGTTTCTTC AGAATTTCTC AATTAATATG 
TTCTGCAAGA TTCCGGTGAG CAAGGTCCAA ACGAATGGTG ACTTGTGCAG TGTTTTGATG 
AAGAGAATAT TTCTATCTGC ACTGCACTTC CGAATTAATA CAAGATACAA GAGTTCAAAT 
CCACCATTCA CTTCAGTTGA ATTGGATCAT AGTGATTCTG GAAGGGAAGG ATGCACTGTC 
ACAACTCTTA CAGTGACTGC AGAACCCAAG AATTGGCAAA ATGCAATTAA AGTTGCTGTT 
CAGGAGGTTC GAAGGCTTAA AGAATTTGGT GTCACAAAGG GTGAATTAAC TCGCTATATG 
GATGCACTTT TAAAAGATAG CGAACATCTG GCAGCTATGA TTGATAATGT ATCATCTGTT 
GATAATTTGG AATTTATCAT GGAGAGTGAT GCTCTTGGCC ATACTGTGAT GGATCAGAGA 
CAAGGACATG AGAGTTTGTT TGCTGTTGCT GGAATGGTCA CACTTGAAGA AGTCAATTCT 
ATTGGTGCCA AGTTATTAGA ATTTATCTCT GATTTTGGAA AACCTACTGC GCCCATTCCT 
GCAGCAATCG TTGCATGTGT TCCTACAAAA GTGCATATTG ATGGATTGGG TGAAACCGAG 
TTCAAGATAT CCTCAAGTGA GATTACAGCT GCCATAAAAT CAGGATTGGA GGAAGCAATT 
GAGGCTGAGC CAGAGCTTGA AGTGCCAAAA GAATTGATAT CTTCAACACA GTTAGAGGAG 
TTAAGGTTAG AACGTAGGCC ATCCTTTGTT CCCTTACTTC CAGATGCAGG CTATACGAAA 
TTGCATGACC AAGAAACAGG CATCACTCAG TGTCGTCTTT CAAATGGAAT TGCTGTAAAT 
TACAAGATAT CCAAAAGTGA ATCCCGGGGA GGTGTCATGC GGCTTATTGT GGGTGGGGGC 
CGAGCAGCTG AAAGTTCCGA GTCTAAAGGA GCTGTTGTTG TGGGTGTTCG AACTCTCAGT 
GAGGGTGGTC GTGTTGGCAG CTTTTCGAGA GAGCAGGTAG AACTTTTTTG TGTGAATCAC 
CTAATAAATT GCTCTTTGGA GTCAACTGAG GAATTTATTT GTATGGAGTT CCGCTTCACT 
CTGCGAGACA ATGGGATGCA AGCAGCATTT GAATTACTTC ATATGGTACT TGAGAATAGT 
GTCTGGCTGG ATGATGCATT TGACAGAGCA AGGCAACTCT ACTTGTCATA TTACCGGTCC 
ATTCCCAAAA GCTTAGAACG GGCAACTGCT CACAAGCTCA TGACAGCAAT GTTGAATGGG 
GATGAGCGGT TCATTGAGCC AACCCCACAA TCATTACAAA ATTTAACACT GAAATCCGTA 
AAAGATGCAG TGATGAATCA GTTTGTTGGT GGAAACATGG AGGTAAGTAT TGTTGGAGAT 
TTCTCAGAGG AGGAGGTTCA GTCTTGCATT ATTGATTACC TGGGCACAGT TAGAGCAACA 
AGAGATTCTG ATCAGGAACA AGAATTTAAT CCTGTCATGT TTCGACCATC CCCTTCTGAT 
TTGCAGTTTC AACAAGTGTT TTTGAAGGAT ACTGATGAGA GAGCATGTGC ATATATTGCT 
GGTCCTGCAC CAAATCGTTG GGGTTTCACT GTTGATGGAA CAGACCTGTT CAAGTCAATG 
AGTGGCTTTT CGGTTTCAGC TGCTGGCCAA TGCTTTGCAG ATGCACAACC AATATCTGAA 
ACACAACAGA TAGACGGGAT GGATGTTCAG AAGGATATGC AAGGAAAACT TCGCTGTCAT 
CCACTCTTCT TTGGCATAAC AATGGGGCTA CTGGCTGAGA TCATAAATTC TCGGCTTTTT 
ACCACAGTTA GGGATTCTCT TGGGTTGACA TATGATGTAT CGTTTGAGTT AAGCCTATTT 
GATAGGCTTA AGCTTGGTTG GTATGTTGTA TCAGTAACAT CAACTCCAGG CAAGGTACAT 
AAAGCGGTTG ATGCATGCAA AAGTGTTCTT AGAGGGTTGC ATAGCAACAA GGTTGCCCAG 
AGAGAGTTGG ACAGGGCAAG ACGGACGCTG CTAATGAGAC ATGAAGCTGA GATTAAGTCC 
AATGCCTATT GGCTTGGATT GCTTGCTCAT TTGCAAGCCT CTTCTGTTCC GAGAAAGGAC 
GTCTCATGTA TTAAAGATCT CACTTCACTC TATGAAGCTG CCACAATTGA GGACATATAC 
CTTGCATATG AACAATTGAA AGTAGATGAG GATTCTCTGT ATTCATGCAT TGGGGTTGCT 
GGGACACAAG CTGGAGAGGA GATTAATGCT CCATTAGAAG TGGAAGAAAC AGATGATGGT 
TTGCAGGGAG GCATACCTGT GGGACGTGGT TTGTCTACAA TGACACGACC AACAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451119.1
CDS	212..4051
Translation	MASTAAPSSVLMTSLPQIRTDDTFSRKNRINLIRSPLIRFQASHHQSCHCISSKRWKHEFAAGRSGTSRKKNNAWKHCSSFLGERVVEASFPEQFKCMSCSLNRRRSRYSIKRSIPRAFIDKSAFRLSGHSFDTASAKHVHVPCASMGPNEPHAASIACPDGILERQDSHLLDSELERARLLEFLNSELPCHPKLHRGQLKNGLCYLILPNKVPPNSFQFFRFEAHMEVHVGSIDEEDDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTCTKDADGDLLPSVLDALNEIAFHPSFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKWDAEKIRKFHERWYFPANATLYIVGDIDNISKTVHQIENVFGQTGLENKTVSAPSPSAFGAMASFLAPKVSVGLPGSSSREKSSSSLDQSKIIKRERHAVRPPVEHYWSLPGSNANLKPPQIFQHEFLQNFSINMFCKIPVSKVQTNGDLCSVLMKRIFLSALHFRINTRYKSSNPPFTSVELDHSDSGREGCTVTTLTVTAEPKNWQNAIKVAVQEVRRLKEFGVTKGELTRYMDALLKDSEHLAAMIDNVSSVDNLEFIMESDALGHTVMDQRQGHESLFAVAGMVTLEEVNSIGAKLLEFISDFGKPTAPIPAAIVACVPTKVHIDGLGETEFKISSSEITAAIKSGLEEAIEAEPELEVPKELISSTQLEELRLERRPSFVPLLPDAGYTKLHDQETGITQCRLSNGIAVNYKISKSESRGGVMRLIVGGGRAAESSESKGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINCSLESTEEFICMEFRFTLRDNGMQAAFELLHMVLENSVWLDDAFDRARQLYLSYYRSIPKSLERATAHKLMTAMLNGDERFIEPTPQSLQNLTLKSVKDAVMNQFVGGNMEVSIVGDFSEEEVQSCIIDYLGTVRATRDSDQEQEFNPVMFRPSPSDLQFQQVFLKDTDERACAYIAGPAPNRWGFTVDGTDLFKSMSGFSVSAAGQCFADAQPISETQQIDGMDVQKDMQGKLRCHPLFFGITMGLLAEIINSRLFTTVRDSLGLTYDVSFELSLFDRLKLGWYVVSVTSTPGKVHKAVDACKSVLRGLHSNKVAQRELDRARRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDVSCIKDLTSLYEAATIEDIYLAYEQLKVDEDSLYSCIGVAGTQAGEEINAPLEVEETDDGLQGGIPVGRGLSTMTRPTT

Target ORF information:

RefSeq Version	XM_024595351.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595351.1

ORF Insert Sequence:

ATGGCGAGTA CAGCTGCCCC AAGCTCTGTA TTGATGACGA GCTTGCCTCA AATCCGTACA 
GATGATACTT TCTCAAGGAA GAACCGGATA AACCTAATTC GTTCCCCTTT GATTCGATTC 
CAAGCCAGCC ATCATCAATC TTGTCATTGC ATTTCCTCCA AAAGGTGGAA ACATGAGTTT 
GCTGCCGGTA GATCAGGTAC TAGTAGAAAG AAGAATAATG CTTGGAAACA TTGTAGTTCG 
TTCCTTGGTG AGAGAGTGGT TGAGGCATCT TTTCCAGAAC AATTTAAGTG CATGTCTTGC 
TCCCTTAACC GCCGTAGAAG CCGCTATAGC ATCAAGAGAT CTATACCTAG AGCCTTTATT 
GATAAGTCTG CTTTTCGTTT GTCTGGCCAT TCATTCGATA CTGCTTCTGC AAAGCATGTT 
CATGTCCCAT GTGCATCAAT GGGTCCAAAT GAGCCACATG CAGCAAGTAT AGCCTGCCCA 
GATGGTATTC TTGAGAGACA AGATTCCCAT TTATTAGATT CTGAATTAGA AAGAGCAAGA 
CTACTTGAGT TTTTGAATTC TGAACTTCCA TGTCACCCCA AGTTACACAG AGGACAACTG 
AAAAATGGAC TCTGTTATCT GATTCTACCA AATAAAGTTC CACCAAACAG CTTTCAATTT 
TTTAGGTTTG AGGCACACAT GGAAGTTCAT GTAGGATCAA TAGACGAGGA AGATGATGAG 
CAAGGAATTG CACATATGAT TGAACATGTT GCATTCCTTG GAAGTAAGAA ACGTGAGAAA 
CTTCTTGGAA CAGGGGCCCG ATCTAATGCT TACACTGATT TCCACCATAC AGTGTTCCAC 
ATTCATTCAC CAACTTGTAC AAAGGATGCT GATGGAGATC TACTCCCCTC TGTGTTGGAT 
GCCTTGAATG AGATAGCTTT CCACCCGAGC TTCCTCGCTT CTCGAGTTGA AAAGGAAAGG 
CGTGCTATAC TTTCAGAACT ACAGATGATG AATACTATAG AATATCGTGT TGACTGCCAG 
TTGTTACAAC ATCTACATTC AGAAAACAAG CTGAGCAAAA GGTTCCCAAT AGGATTGGAA 
GAGCAGATTA AGAAGTGGGA TGCAGAGAAA ATTAGGAAAT TCCATGAGCG TTGGTACTTC 
CCAGCAAATG CAACTCTATA CATTGTTGGT GACATTGATA ACATTTCAAA GACGGTTCAC 
CAAATTGAAA ATGTCTTTGG ACAAACTGGC CTGGAAAACA AGACAGTTTC TGCTCCTTCT 
CCAAGTGCAT TTGGTGCAAT GGCTAGTTTT CTTGCTCCTA AGGTCTCAGT TGGGCTCCCT 
GGAAGTTCAT CTCGTGAAAA GTCATCTAGT TCTCTAGACC AATCCAAAAT CATTAAAAGG 
GAAAGGCATG CAGTCCGTCC TCCTGTCGAG CATTATTGGT CTCTTCCTGG AAGCAATGCA 
AATTTGAAGC CACCACAGAT ATTTCAGCAT GAGTTTCTTC AGAATTTCTC AATTAATATG 
TTCTGCAAGA TTCCGGTGAG CAAGGTCCAA ACGAATGGTG ACTTGTGCAG TGTTTTGATG 
AAGAGAATAT TTCTATCTGC ACTGCACTTC CGAATTAATA CAAGATACAA GAGTTCAAAT 
CCACCATTCA CTTCAGTTGA ATTGGATCAT AGTGATTCTG GAAGGGAAGG ATGCACTGTC 
ACAACTCTTA CAGTGACTGC AGAACCCAAG AATTGGCAAA ATGCAATTAA AGTTGCTGTT 
CAGGAGGTTC GAAGGCTTAA AGAATTTGGT GTCACAAAGG GTGAATTAAC TCGCTATATG 
GATGCACTTT TAAAAGATAG CGAACATCTG GCAGCTATGA TTGATAATGT ATCATCTGTT 
GATAATTTGG AATTTATCAT GGAGAGTGAT GCTCTTGGCC ATACTGTGAT GGATCAGAGA 
CAAGGACATG AGAGTTTGTT TGCTGTTGCT GGAATGGTCA CACTTGAAGA AGTCAATTCT 
ATTGGTGCCA AGTTATTAGA ATTTATCTCT GATTTTGGAA AACCTACTGC GCCCATTCCT 
GCAGCAATCG TTGCATGTGT TCCTACAAAA GTGCATATTG ATGGATTGGG TGAAACCGAG 
TTCAAGATAT CCTCAAGTGA GATTACAGCT GCCATAAAAT CAGGATTGGA GGAAGCAATT 
GAGGCTGAGC CAGAGCTTGA AGTGCCAAAA GAATTGATAT CTTCAACACA GTTAGAGGAG 
TTAAGGTTAG AACGTAGGCC ATCCTTTGTT CCCTTACTTC CAGATGCAGG CTATACGAAA 
TTGCATGACC AAGAAACAGG CATCACTCAG TGTCGTCTTT CAAATGGAAT TGCTGTAAAT 
TACAAGATAT CCAAAAGTGA ATCCCGGGGA GGTGTCATGC GGCTTATTGT GGGTGGGGGC 
CGAGCAGCTG AAAGTTCCGA GTCTAAAGGA GCTGTTGTTG TGGGTGTTCG AACTCTCAGT 
GAGGGTGGTC GTGTTGGCAG CTTTTCGAGA GAGCAGGTAG AACTTTTTTG TGTGAATCAC 
CTAATAAATT GCTCTTTGGA GTCAACTGAG GAATTTATTT GTATGGAGTT CCGCTTCACT 
CTGCGAGACA ATGGGATGCA AGCAGCATTT GAATTACTTC ATATGGTACT TGAGAATAGT 
GTCTGGCTGG ATGATGCATT TGACAGAGCA AGGCAACTCT ACTTGTCATA TTACCGGTCC 
ATTCCCAAAA GCTTAGAACG GGCAACTGCT CACAAGCTCA TGACAGCAAT GTTGAATGGG 
GATGAGCGGT TCATTGAGCC AACCCCACAA TCATTACAAA ATTTAACACT GAAATCCGTA 
AAAGATGCAG TGATGAATCA GTTTGTTGGT GGAAACATGG AGGTAAGTAT TGTTGGAGAT 
TTCTCAGAGG AGGAGGTTCA GTCTTGCATT ATTGATTACC TGGGCACAGT TAGAGCAACA 
AGAGATTCTG ATCAGGAACA AGAATTTAAT CCTGTCATGT TTCGACCATC CCCTTCTGAT 
TTGCAGTTTC AACAAGTGTT TTTGAAGGAT ACTGATGAGA GAGCATGTGC ATATATTGCT 
GGTCCTGCAC CAAATCGTTG GGGTTTCACT GTTGATGGAA CAGACCTGTT CAAGTCAATG 
AGTGGCTTTT CGGTTTCAGC TGCTGGCCAA TGCTTTGCAG ATGCACAACC AATATCTGAA 
ACACAACAGA TAGACGGGAT GGATGTTCAG AAGGATATGC AAGGAAAACT TCGCTGTCAT 
CCACTCTTCT TTGGCATAAC AATGGGGCTA CTGGCTGAGA TCATAAATTC TCGGCTTTTT 
ACCACAGTTA GGGATTCTCT TGGGTTGACA TATGATGTAT CGTTTGAGTT AAGCCTATTT 
GATAGGCTTA AGCTTGGTTG GTATGTTGTA TCAGTAACAT CAACTCCAGG CAAGGTACAT 
AAAGCGGTTG ATGCATGCAA AAGTGTTCTT AGAGGGTTGC ATAGCAACAA GGTTGCCCAG 
AGAGAGTTGG ACAGGGCAAG ACGGACGCTG CTAATGAGAC ATGAAGCTGA GATTAAGTCC 
AATGCCTATT GGCTTGGATT GCTTGCTCAT TTGCAAGCCT CTTCTGTTCC GAGAAAGGAC 
GTCTCATGTA TTAAAGATCT CACTTCACTC TATGAAGCTG CCACAATTGA GGACATATAC 
CTTGCATATG AACAATTGAA AGTAGATGAG GATTCTCTGT ATTCATGCAT TGGGGTTGCT 
GGGACACAAG CTGGAGAGGA GATTAATGCT CCATTAGAAG TGGAAGAAAC AGATGATGGT 
TTGCAGGGAG GCATACCTGT GGGACGTGGT TTGTCTACAA TGACACGACC AACAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7488593 ( XM_024595352.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451120.1 Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X3, mRNA.

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CloneID	OPa424733
Clone ID Related Accession (Same CDS sequence)	XM_024595352.1
Accession Version	XM_024595352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	stromal processing peptidase, chloroplastic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGTA CAGCTGCCCC AAGCTCTGTA TTGATGACGA GCTTGCCTCA AATCCGTACA 
GATGATACTT TCTCAAGGAA GAACCGGATA AACCTAATTC GTTCCCCTTT GATTCGATTC 
CAAGCCAGCC ATCATCAATC TTGTCATTGC ATTTCCTCCA AAAGGTGGAA ACATGAGTTT 
GCTGCCGGTA GATCAGGTAC TAGTAGAAAG AAGAATAATG CTTGGAAACA TTGTAGTTCG 
TTCCTTGGTG AGAGAGTGGT TGAGGCATCT TTTCCAGAAC AATTTAAGTG CATGTCTTGC 
TCCCTTAACC GCCGTAGAAG CCGCTATAGC ATCAAGAGAT CTATACCTAG AGCCTTTATT 
GATAAGTCTG CTTTTCGTTT GTCTGGCCAT TCATTCGATA CTGCTTCTGC AAAGCATGTT 
CATGTCCCAT GTGCATCAAT GGGTCCAAAT GAGCCACATG CAGCAAGTAT AGCCTGCCCA 
GATGGTATTC TTGAGAGACA AGATTCCCAT TTATTAGATT CTGAATTAGA AAGAGCAAGA 
CTACTTGAGT TTTTGAATTC TGAACTTCCA TGTCACCCCA AGTTACACAG AGGACAACTG 
AAAAATGGAC TCTGTTATCT GATTCTACCA AATAAAGTTC CACCAAACAG GTTTGAGGCA 
CACATGGAAG TTCATGTAGG ATCAATAGAC GAGGAAGATG ATGAGCAAGG AATTGCACAT 
ATGATTGAAC ATGTTGCATT CCTTGGAAGT AAGAAACGTG AGAAACTTCT TGGAACAGGG 
GCCCGATCTA ATGCTTACAC TGATTTCCAC CATACAGTGT TCCACATTCA TTCACCAACT 
TGTACAAAGG ATGCTGATGG AGATCTACTC CCCTCTGTGT TGGATGCCTT GAATGAGATA 
GCTTTCCACC CGAGCTTCCT CGCTTCTCGA GTTGAAAAGG AAAGGCGTGC TATACTTTCA 
GAACTACAGA TGATGAATAC TATAGAATAT CGTGTTGACT GCCAGTTGTT ACAACATCTA 
CATTCAGAAA ACAAGCTGAG CAAAAGGTTC CCAATAGGAT TGGAAGAGCA GATTAAGAAG 
TGGGATGCAG AGAAAATTAG GAAATTCCAT GAGCGTTGGT ACTTCCCAGC AAATGCAACT 
CTATACATTG TTGGTGACAT TGATAACATT TCAAAGACGG TTCACCAAAT TGAAAATGTC 
TTTGGACAAA CTGGCCTGGA AAACAAGACA GTTTCTGCTC CTTCTCCAAG TGCATTTGGT 
GCAATGGCTA GTTTTCTTGC TCCTAAGGTC TCAGTTGGGC TCCCTGGAAG TTCATCTCGT 
GAAAAGTCAT CTAGTTCTCT AGACCAATCC AAAATCATTA AAAGGGAAAG GCATGCAGTC 
CGTCCTCCTG TCGAGCATTA TTGGTCTCTT CCTGGAAGCA ATGCAAATTT GAAGCCACCA 
CAGATATTTC AGCATGAGTT TCTTCAGAAT TTCTCAATTA ATATGTTCTG CAAGATTCCG 
GTGAGCAAGG TCCAAACGAA TGGTGACTTG TGCAGTGTTT TGATGAAGAG AATATTTCTA 
TCTGCACTGC ACTTCCGAAT TAATACAAGA TACAAGAGTT CAAATCCACC ATTCACTTCA 
GTTGAATTGG ATCATAGTGA TTCTGGAAGG GAAGGATGCA CTGTCACAAC TCTTACAGTG 
ACTGCAGAAC CCAAGAATTG GCAAAATGCA ATTAAAGTTG CTGTTCAGGA GGTTCGAAGG 
CTTAAAGAAT TTGGTGTCAC AAAGGGTGAA TTAACTCGCT ATATGGATGC ACTTTTAAAA 
GATAGCGAAC ATCTGGCAGC TATGATTGAT AATGTATCAT CTGTTGATAA TTTGGAATTT 
ATCATGGAGA GTGATGCTCT TGGCCATACT GTGATGGATC AGAGACAAGG ACATGAGAGT 
TTGTTTGCTG TTGCTGGAAT GGTCACACTT GAAGAAGTCA ATTCTATTGG TGCCAAGTTA 
TTAGAATTTA TCTCTGATTT TGGAAAACCT ACTGCGCCCA TTCCTGCAGC AATCGTTGCA 
TGTGTTCCTA CAAAAGTGCA TATTGATGGA TTGGGTGAAA CCGAGTTCAA GATATCCTCA 
AGTGAGATTA CAGCTGCCAT AAAATCAGGA TTGGAGGAAG CAATTGAGGC TGAGCCAGAG 
CTTGAAGTGC CAAAAGAATT GATATCTTCA ACACAGTTAG AGGAGTTAAG GTTAGAACGT 
AGGCCATCCT TTGTTCCCTT ACTTCCAGAT GCAGGCTATA CGAAATTGCA TGACCAAGAA 
ACAGGCATCA CTCAGTGTCG TCTTTCAAAT GGAATTGCTG TAAATTACAA GATATCCAAA 
AGTGAATCCC GGGGAGGTGT CATGCGGCTT ATTGTGGGTG GGGGCCGAGC AGCTGAAAGT 
TCCGAGTCTA AAGGAGCTGT TGTTGTGGGT GTTCGAACTC TCAGTGAGGG TGGTCGTGTT 
GGCAGCTTTT CGAGAGAGCA GGTAGAACTT TTTTGTGTGA ATCACCTAAT AAATTGCTCT 
TTGGAGTCAA CTGAGGAATT TATTTGTATG GAGTTCCGCT TCACTCTGCG AGACAATGGG 
ATGCAAGCAG CATTTGAATT ACTTCATATG GTACTTGAGA ATAGTGTCTG GCTGGATGAT 
GCATTTGACA GAGCAAGGCA ACTCTACTTG TCATATTACC GGTCCATTCC CAAAAGCTTA 
GAACGGGCAA CTGCTCACAA GCTCATGACA GCAATGTTGA ATGGGGATGA GCGGTTCATT 
GAGCCAACCC CACAATCATT ACAAAATTTA ACACTGAAAT CCGTAAAAGA TGCAGTGATG 
AATCAGTTTG TTGGTGGAAA CATGGAGGTA AGTATTGTTG GAGATTTCTC AGAGGAGGAG 
GTTCAGTCTT GCATTATTGA TTACCTGGGC ACAGTTAGAG CAACAAGAGA TTCTGATCAG 
GAACAAGAAT TTAATCCTGT CATGTTTCGA CCATCCCCTT CTGATTTGCA GTTTCAACAA 
GTGTTTTTGA AGGATACTGA TGAGAGAGCA TGTGCATATA TTGCTGGTCC TGCACCAAAT 
CGTTGGGGTT TCACTGTTGA TGGAACAGAC CTGTTCAAGT CAATGAGTGG CTTTTCGGTT 
TCAGCTGCTG GCCAATGCTT TGCAGATGCA CAACCAATAT CTGAAACACA ACAGATAGAC 
GGGATGGATG TTCAGAAGGA TATGCAAGGA AAACTTCGCT GTCATCCACT CTTCTTTGGC 
ATAACAATGG GGCTACTGGC TGAGATCATA AATTCTCGGC TTTTTACCAC AGTTAGGGAT 
TCTCTTGGGT TGACATATGA TGTATCGTTT GAGTTAAGCC TATTTGATAG GCTTAAGCTT 
GGTTGGTATG TTGTATCAGT AACATCAACT CCAGGCAAGG TACATAAAGC GGTTGATGCA 
TGCAAAAGTG TTCTTAGAGG GTTGCATAGC AACAAGGTTG CCCAGAGAGA GTTGGACAGG 
GCAAGACGGA CGCTGCTAAT GAGACATGAA GCTGAGATTA AGTCCAATGC CTATTGGCTT 
GGATTGCTTG CTCATTTGCA AGCCTCTTCT GTTCCGAGAA AGGACGTCTC ATGTATTAAA 
GATCTCACTT CACTCTATGA AGCTGCCACA ATTGAGGACA TATACCTTGC ATATGAACAA 
TTGAAAGTAG ATGAGGATTC TCTGTATTCA TGCATTGGGG TTGCTGGGAC ACAAGCTGGA 
GAGGAGATTA ATGCTCCATT AGAAGTGGAA GAAACAGATG ATGGTTTGCA GGGAGGCATA 
CCTGTGGGAC GTGGTTTGTC TACAATGACA CGACCAACAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451120.1
CDS	212..4036
Translation	MASTAAPSSVLMTSLPQIRTDDTFSRKNRINLIRSPLIRFQASHHQSCHCISSKRWKHEFAAGRSGTSRKKNNAWKHCSSFLGERVVEASFPEQFKCMSCSLNRRRSRYSIKRSIPRAFIDKSAFRLSGHSFDTASAKHVHVPCASMGPNEPHAASIACPDGILERQDSHLLDSELERARLLEFLNSELPCHPKLHRGQLKNGLCYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTCTKDADGDLLPSVLDALNEIAFHPSFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKWDAEKIRKFHERWYFPANATLYIVGDIDNISKTVHQIENVFGQTGLENKTVSAPSPSAFGAMASFLAPKVSVGLPGSSSREKSSSSLDQSKIIKRERHAVRPPVEHYWSLPGSNANLKPPQIFQHEFLQNFSINMFCKIPVSKVQTNGDLCSVLMKRIFLSALHFRINTRYKSSNPPFTSVELDHSDSGREGCTVTTLTVTAEPKNWQNAIKVAVQEVRRLKEFGVTKGELTRYMDALLKDSEHLAAMIDNVSSVDNLEFIMESDALGHTVMDQRQGHESLFAVAGMVTLEEVNSIGAKLLEFISDFGKPTAPIPAAIVACVPTKVHIDGLGETEFKISSSEITAAIKSGLEEAIEAEPELEVPKELISSTQLEELRLERRPSFVPLLPDAGYTKLHDQETGITQCRLSNGIAVNYKISKSESRGGVMRLIVGGGRAAESSESKGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINCSLESTEEFICMEFRFTLRDNGMQAAFELLHMVLENSVWLDDAFDRARQLYLSYYRSIPKSLERATAHKLMTAMLNGDERFIEPTPQSLQNLTLKSVKDAVMNQFVGGNMEVSIVGDFSEEEVQSCIIDYLGTVRATRDSDQEQEFNPVMFRPSPSDLQFQQVFLKDTDERACAYIAGPAPNRWGFTVDGTDLFKSMSGFSVSAAGQCFADAQPISETQQIDGMDVQKDMQGKLRCHPLFFGITMGLLAEIINSRLFTTVRDSLGLTYDVSFELSLFDRLKLGWYVVSVTSTPGKVHKAVDACKSVLRGLHSNKVAQRELDRARRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDVSCIKDLTSLYEAATIEDIYLAYEQLKVDEDSLYSCIGVAGTQAGEEINAPLEVEETDDGLQGGIPVGRGLSTMTRPTT

Target ORF information:

RefSeq Version	XM_024595352.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595352.1

ORF Insert Sequence:

ATGGCGAGTA CAGCTGCCCC AAGCTCTGTA TTGATGACGA GCTTGCCTCA AATCCGTACA 
GATGATACTT TCTCAAGGAA GAACCGGATA AACCTAATTC GTTCCCCTTT GATTCGATTC 
CAAGCCAGCC ATCATCAATC TTGTCATTGC ATTTCCTCCA AAAGGTGGAA ACATGAGTTT 
GCTGCCGGTA GATCAGGTAC TAGTAGAAAG AAGAATAATG CTTGGAAACA TTGTAGTTCG 
TTCCTTGGTG AGAGAGTGGT TGAGGCATCT TTTCCAGAAC AATTTAAGTG CATGTCTTGC 
TCCCTTAACC GCCGTAGAAG CCGCTATAGC ATCAAGAGAT CTATACCTAG AGCCTTTATT 
GATAAGTCTG CTTTTCGTTT GTCTGGCCAT TCATTCGATA CTGCTTCTGC AAAGCATGTT 
CATGTCCCAT GTGCATCAAT GGGTCCAAAT GAGCCACATG CAGCAAGTAT AGCCTGCCCA 
GATGGTATTC TTGAGAGACA AGATTCCCAT TTATTAGATT CTGAATTAGA AAGAGCAAGA 
CTACTTGAGT TTTTGAATTC TGAACTTCCA TGTCACCCCA AGTTACACAG AGGACAACTG 
AAAAATGGAC TCTGTTATCT GATTCTACCA AATAAAGTTC CACCAAACAG GTTTGAGGCA 
CACATGGAAG TTCATGTAGG ATCAATAGAC GAGGAAGATG ATGAGCAAGG AATTGCACAT 
ATGATTGAAC ATGTTGCATT CCTTGGAAGT AAGAAACGTG AGAAACTTCT TGGAACAGGG 
GCCCGATCTA ATGCTTACAC TGATTTCCAC CATACAGTGT TCCACATTCA TTCACCAACT 
TGTACAAAGG ATGCTGATGG AGATCTACTC CCCTCTGTGT TGGATGCCTT GAATGAGATA 
GCTTTCCACC CGAGCTTCCT CGCTTCTCGA GTTGAAAAGG AAAGGCGTGC TATACTTTCA 
GAACTACAGA TGATGAATAC TATAGAATAT CGTGTTGACT GCCAGTTGTT ACAACATCTA 
CATTCAGAAA ACAAGCTGAG CAAAAGGTTC CCAATAGGAT TGGAAGAGCA GATTAAGAAG 
TGGGATGCAG AGAAAATTAG GAAATTCCAT GAGCGTTGGT ACTTCCCAGC AAATGCAACT 
CTATACATTG TTGGTGACAT TGATAACATT TCAAAGACGG TTCACCAAAT TGAAAATGTC 
TTTGGACAAA CTGGCCTGGA AAACAAGACA GTTTCTGCTC CTTCTCCAAG TGCATTTGGT 
GCAATGGCTA GTTTTCTTGC TCCTAAGGTC TCAGTTGGGC TCCCTGGAAG TTCATCTCGT 
GAAAAGTCAT CTAGTTCTCT AGACCAATCC AAAATCATTA AAAGGGAAAG GCATGCAGTC 
CGTCCTCCTG TCGAGCATTA TTGGTCTCTT CCTGGAAGCA ATGCAAATTT GAAGCCACCA 
CAGATATTTC AGCATGAGTT TCTTCAGAAT TTCTCAATTA ATATGTTCTG CAAGATTCCG 
GTGAGCAAGG TCCAAACGAA TGGTGACTTG TGCAGTGTTT TGATGAAGAG AATATTTCTA 
TCTGCACTGC ACTTCCGAAT TAATACAAGA TACAAGAGTT CAAATCCACC ATTCACTTCA 
GTTGAATTGG ATCATAGTGA TTCTGGAAGG GAAGGATGCA CTGTCACAAC TCTTACAGTG 
ACTGCAGAAC CCAAGAATTG GCAAAATGCA ATTAAAGTTG CTGTTCAGGA GGTTCGAAGG 
CTTAAAGAAT TTGGTGTCAC AAAGGGTGAA TTAACTCGCT ATATGGATGC ACTTTTAAAA 
GATAGCGAAC ATCTGGCAGC TATGATTGAT AATGTATCAT CTGTTGATAA TTTGGAATTT 
ATCATGGAGA GTGATGCTCT TGGCCATACT GTGATGGATC AGAGACAAGG ACATGAGAGT 
TTGTTTGCTG TTGCTGGAAT GGTCACACTT GAAGAAGTCA ATTCTATTGG TGCCAAGTTA 
TTAGAATTTA TCTCTGATTT TGGAAAACCT ACTGCGCCCA TTCCTGCAGC AATCGTTGCA 
TGTGTTCCTA CAAAAGTGCA TATTGATGGA TTGGGTGAAA CCGAGTTCAA GATATCCTCA 
AGTGAGATTA CAGCTGCCAT AAAATCAGGA TTGGAGGAAG CAATTGAGGC TGAGCCAGAG 
CTTGAAGTGC CAAAAGAATT GATATCTTCA ACACAGTTAG AGGAGTTAAG GTTAGAACGT 
AGGCCATCCT TTGTTCCCTT ACTTCCAGAT GCAGGCTATA CGAAATTGCA TGACCAAGAA 
ACAGGCATCA CTCAGTGTCG TCTTTCAAAT GGAATTGCTG TAAATTACAA GATATCCAAA 
AGTGAATCCC GGGGAGGTGT CATGCGGCTT ATTGTGGGTG GGGGCCGAGC AGCTGAAAGT 
TCCGAGTCTA AAGGAGCTGT TGTTGTGGGT GTTCGAACTC TCAGTGAGGG TGGTCGTGTT 
GGCAGCTTTT CGAGAGAGCA GGTAGAACTT TTTTGTGTGA ATCACCTAAT AAATTGCTCT 
TTGGAGTCAA CTGAGGAATT TATTTGTATG GAGTTCCGCT TCACTCTGCG AGACAATGGG 
ATGCAAGCAG CATTTGAATT ACTTCATATG GTACTTGAGA ATAGTGTCTG GCTGGATGAT 
GCATTTGACA GAGCAAGGCA ACTCTACTTG TCATATTACC GGTCCATTCC CAAAAGCTTA 
GAACGGGCAA CTGCTCACAA GCTCATGACA GCAATGTTGA ATGGGGATGA GCGGTTCATT 
GAGCCAACCC CACAATCATT ACAAAATTTA ACACTGAAAT CCGTAAAAGA TGCAGTGATG 
AATCAGTTTG TTGGTGGAAA CATGGAGGTA AGTATTGTTG GAGATTTCTC AGAGGAGGAG 
GTTCAGTCTT GCATTATTGA TTACCTGGGC ACAGTTAGAG CAACAAGAGA TTCTGATCAG 
GAACAAGAAT TTAATCCTGT CATGTTTCGA CCATCCCCTT CTGATTTGCA GTTTCAACAA 
GTGTTTTTGA AGGATACTGA TGAGAGAGCA TGTGCATATA TTGCTGGTCC TGCACCAAAT 
CGTTGGGGTT TCACTGTTGA TGGAACAGAC CTGTTCAAGT CAATGAGTGG CTTTTCGGTT 
TCAGCTGCTG GCCAATGCTT TGCAGATGCA CAACCAATAT CTGAAACACA ACAGATAGAC 
GGGATGGATG TTCAGAAGGA TATGCAAGGA AAACTTCGCT GTCATCCACT CTTCTTTGGC 
ATAACAATGG GGCTACTGGC TGAGATCATA AATTCTCGGC TTTTTACCAC AGTTAGGGAT 
TCTCTTGGGT TGACATATGA TGTATCGTTT GAGTTAAGCC TATTTGATAG GCTTAAGCTT 
GGTTGGTATG TTGTATCAGT AACATCAACT CCAGGCAAGG TACATAAAGC GGTTGATGCA 
TGCAAAAGTG TTCTTAGAGG GTTGCATAGC AACAAGGTTG CCCAGAGAGA GTTGGACAGG 
GCAAGACGGA CGCTGCTAAT GAGACATGAA GCTGAGATTA AGTCCAATGC CTATTGGCTT 
GGATTGCTTG CTCATTTGCA AGCCTCTTCT GTTCCGAGAA AGGACGTCTC ATGTATTAAA 
GATCTCACTT CACTCTATGA AGCTGCCACA ATTGAGGACA TATACCTTGC ATATGAACAA 
TTGAAAGTAG ATGAGGATTC TCTGTATTCA TGCATTGGGG TTGCTGGGAC ACAAGCTGGA 
GAGGAGATTA ATGCTCCATT AGAAGTGGAA GAAACAGATG ATGGTTTGCA GGGAGGCATA 
CCTGTGGGAC GTGGTTTGTC TACAATGACA CGACCAACAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7488593 ( XM_024595353.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451121.1 Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPa424734
Clone ID Related Accession (Same CDS sequence)	XM_024595353.1
Accession Version	XM_024595353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	stromal processing peptidase, chloroplastic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGTA CAGCTGCCCC AAGCTCTGTA TTGATGACGA GCTTGCCTCA AATCCGTACA 
GATGATACTT TCTCAAGGAA GAACCGGATA AACCTAATTC GTTCCCCTTT GATTCGATTC 
CAAGCCAGCC ATCATCAATC TTGTCATTGC ATTTCCTCCA AAAGGTGGAA ACATGAGTTT 
GCTGCCGGTA GATCAGGTAC TAGTAGAAAG AAGAATAATG CTTGGAAACA TTGTAGTTCG 
TTCCTTGGTG AGAGAGTGGT TGAGGCATCT TTTCCAGAAC AATTTAAGTG CATGTCTTGC 
TCCCTTAACC GCCGTAGAAG CCGCTATAGC ATCAAGAGAT CTATACCTAG AGCCTTTATT 
GATAAGTCTG CTTTTCGTTT GTCTGGCCAT TCATTCGATA CTGCTTCTGC AAAGCATGTT 
CATGTCCCAT GTGCATCAAT GGGTCCAAAT GAGCCACATG CAGCAAGTAT AGCCTGCCCA 
GATGGTATTC TTGAGAGACA AGATTCCCAT TTATTAGATT CTGAATTAGA AAGAGCAAGA 
CTACTTGAGT TTTTGAATTC TGAACTTCCA TGTCACCCCA AGTTACACAG AGGACAACTG 
AAAAATGGAC TCTGTTATCT GATTCTACCA AATAAAGTTC CACCAAACAG CTTTCAATTT 
TTTAGGTTTG AGGCACACAT GGAAGTTCAT GTAGGATCAA TAGACGAGGA AGATGATGAG 
CAAGGAATTG CACATATGAT TGAACATGTT GCATTCCTTG GAAGTAAGAA ACGTGAGAAA 
CTTCTTGGAA CAGGGGCCCG ATCTAATGCT TACACTGATT TCCACCATAC AGTGTTCCAC 
ATTCATTCAC CAACTTGTAC AAAGGATGCT GATGGAGATC TACTCCCCTC TGTGTTGGAT 
GCCTTGAATG AGATAGCTTT CCACCCGAGC TTCCTCGCTT CTCGAGTTGA AAAGGAAAGG 
CGTGCTATAC TTTCAGAACT ACAGATGATG AATACTATAG AATATCGTGT TGACTGCCAG 
TTGTTACAAC ATCTACATTC AGAAAACAAG CTGAGCAAAA GGTTCCCAAT AGGATTGGAA 
GAGCAGATTA AGAAGTGGGA TGCAGAGAAA ATTAGGAAAT TCCATGAGCG TTGGTACTTC 
CCAGCAAATG CAACTCTATA CATTGTTGGT GACATTGATA ACATTTCAAA GACGGTTCAC 
CAAATTGAAA ATGTCTTTGG ACAAACTGGC CTGGAAAACA AGACAGTTTC TGCTCCTTCT 
CCAAGTGCAT TTGGTGCAAT GGCTAGTTTT CTTGCTCCTA AGGTCTCAGT TGGGCTCCCT 
GGAAGTTCAT CTCGTGAAAA GTCATCTAGT TCTCTAGACC AATCCAAAAT CATTAAAAGG 
GAAAGGCATG CAGTCCGTCC TCCTGTCGAG CATTATTGGT CTCTTCCTGG AAGCAATGCA 
AATTTGAAGC CACCACAGAT ATTTCAGCAT GAGTTTCTTC AGAATTTCTC AATTAATATG 
TTCTGCAAGA TTCCGGTGAG CAAGGTCCAA ACGAATGGTG ACTTGTGCAG TGTTTTGATG 
AAGAGAATAT TTCTATCTGC ACTGCACTTC CGAATTAATA CAAGATACAA GAGTTCAAAT 
CCACCATTCA CTTCAGTTGA ATTGGATCAT AGTGATTCTG GAAGGGAAGG ATGCACTGTC 
ACAACTCTTA CAGTGACTGC AGAACCCAAG AATTGGCAAA ATGCAATTAA AGTTGCTGTT 
CAGGAGGTTC GAAGGCTTAA AGAATTTGGT GTCACAAAGG GTGAATTAAC TCGCTATATG 
GATGCACTTT TAAAAGATAG CGAACATCTG GCAGCTATGA TTGATAATGT ATCATCTGTT 
GATAATTTGG AATTTATCAT GGAGAGTGAT GCTCTTGGCC ATACTGTGAT GGATCAGAGA 
CAAGGACATG AGAGTTTGTT TGCTGTTGCT GGAATGGTCA CACTTGAAGA AGTCAATTCT 
ATTGGTGCCA AGTTATTAGA ATTTATCTCT GATTTTGGAA AACCTACTGC GCCCATTCCT 
GCAGCAATCG TTGCATGTGT TCCTACAAAA GTGCATATTG ATGGATTGGG TGAAACCGAG 
TTCAAGATAT CCTCAAGTGA GATTACAGCT GCCATAAAAT CAGGATTGGA GGAAGCAATT 
GAGGCTGAGC CAGAGCTTGA AGTGCCAAAA GAATTGATAT CTTCAACACA GTTAGAGGAG 
TTAAGGTTAG AACGTAGGCC ATCCTTTGTT CCCTTACTTC CAGATGCAGG CTATACGAAA 
TTGCATGACC AAGAAACAGG CATCACTCAG TGTCGTCTTT CAAATGGAAT TGCTGTAAAT 
TACAAGATAT CCAAAAGTGA ATCCCGGGGA GGTGTCATGC GGCTTATTGT GGGTGGGGGC 
CGAGCAGCTG AAAGTTCCGA GTCTAAAGGA GCTGTTGTTG TGGGTGTTCG AACTCTCAGT 
GAGGGTGGTC GTGTTGGCAG CTTTTCGAGA GAGCAGGTAG AACTTTTTTG TGTGAATCAC 
CTAATAAATT GCTCTTTGGA GTCAACTGAG GAATTTATTT GTATGGAGTT CCGCTTCACT 
CTGCGAGACA ATGGGATGCA AGCAGCATTT GAATTACTTC ATATGGTACT TGAGAATAGT 
GTCTGGCTGG ATGATGCATT TGACAGAGCA AGGCAACTCT ACTTGTCATA TTACCGGTCC 
ATTCCCAAAA GCTTAGAACG GGCAACTGCT CACAAGCTCA TGACAGCAAT GTTGAATGGG 
GATGAGCGGT TCATTGAGCC AACCCCACAA TCATTACAAA ATTTAACACT GAAATCCGTA 
AAAGATGCAG TGATGAATCA GTTTGTTGGT GGAAACATGG AGGTAAGTAT TGTTGGAGAT 
TTCTCAGAGG AGGAGGTTCA GTCTTGCATT ATTGATTACC TGGGCACAGT TAGAGCAACA 
AGAGATTCTG ATCAGGAACA AGAATTTAAT CCTGTCATGT TTCGACCATC CCCTTCTGAT 
TTGCAGTTTC AACAAGTGTT TTTGAAGGAT ACTGATGAGA GAGCATGTGC ATATATTGCT 
GGTCCTGCAC CAAATCGTTG GGGTTTCACT GTTGATGGAA CAGACCTGTT CAAGTCAATG 
AGTGGCTTTT CGGTTTCAGC TGATGCACAA CCAATATCTG AAACACAACA GATAGACGGG 
ATGGATGTTC AGAAGGATAT GCAAGGAAAA CTTCGCTGTC ATCCACTCTT CTTTGGCATA 
ACAATGGGGC TACTGGCTGA GATCATAAAT TCTCGGCTTT TTACCACAGT TAGGGATTCT 
CTTGGGTTGA CATATGATGT ATCGTTTGAG TTAAGCCTAT TTGATAGGCT TAAGCTTGGT 
TGGTATGTTG TATCAGTAAC ATCAACTCCA GGCAAGGTAC ATAAAGCGGT TGATGCATGC 
AAAAGTGTTC TTAGAGGGTT GCATAGCAAC AAGGTTGCCC AGAGAGAGTT GGACAGGGCA 
AGACGGACGC TGCTAATGAG ACATGAAGCT GAGATTAAGT CCAATGCCTA TTGGCTTGGA 
TTGCTTGCTC ATTTGCAAGC CTCTTCTGTT CCGAGAAAGG ACGTCTCATG TATTAAAGAT 
CTCACTTCAC TCTATGAAGC TGCCACAATT GAGGACATAT ACCTTGCATA TGAACAATTG 
AAAGTAGATG AGGATTCTCT GTATTCATGC ATTGGGGTTG CTGGGACACA AGCTGGAGAG 
GAGATTAATG CTCCATTAGA AGTGGAAGAA ACAGATGATG GTTTGCAGGG AGGCATACCT 
GTGGGACGTG GTTTGTCTAC AATGACACGA CCAACAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451121.1
CDS	212..4033
Translation	MASTAAPSSVLMTSLPQIRTDDTFSRKNRINLIRSPLIRFQASHHQSCHCISSKRWKHEFAAGRSGTSRKKNNAWKHCSSFLGERVVEASFPEQFKCMSCSLNRRRSRYSIKRSIPRAFIDKSAFRLSGHSFDTASAKHVHVPCASMGPNEPHAASIACPDGILERQDSHLLDSELERARLLEFLNSELPCHPKLHRGQLKNGLCYLILPNKVPPNSFQFFRFEAHMEVHVGSIDEEDDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTCTKDADGDLLPSVLDALNEIAFHPSFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKWDAEKIRKFHERWYFPANATLYIVGDIDNISKTVHQIENVFGQTGLENKTVSAPSPSAFGAMASFLAPKVSVGLPGSSSREKSSSSLDQSKIIKRERHAVRPPVEHYWSLPGSNANLKPPQIFQHEFLQNFSINMFCKIPVSKVQTNGDLCSVLMKRIFLSALHFRINTRYKSSNPPFTSVELDHSDSGREGCTVTTLTVTAEPKNWQNAIKVAVQEVRRLKEFGVTKGELTRYMDALLKDSEHLAAMIDNVSSVDNLEFIMESDALGHTVMDQRQGHESLFAVAGMVTLEEVNSIGAKLLEFISDFGKPTAPIPAAIVACVPTKVHIDGLGETEFKISSSEITAAIKSGLEEAIEAEPELEVPKELISSTQLEELRLERRPSFVPLLPDAGYTKLHDQETGITQCRLSNGIAVNYKISKSESRGGVMRLIVGGGRAAESSESKGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINCSLESTEEFICMEFRFTLRDNGMQAAFELLHMVLENSVWLDDAFDRARQLYLSYYRSIPKSLERATAHKLMTAMLNGDERFIEPTPQSLQNLTLKSVKDAVMNQFVGGNMEVSIVGDFSEEEVQSCIIDYLGTVRATRDSDQEQEFNPVMFRPSPSDLQFQQVFLKDTDERACAYIAGPAPNRWGFTVDGTDLFKSMSGFSVSADAQPISETQQIDGMDVQKDMQGKLRCHPLFFGITMGLLAEIINSRLFTTVRDSLGLTYDVSFELSLFDRLKLGWYVVSVTSTPGKVHKAVDACKSVLRGLHSNKVAQRELDRARRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDVSCIKDLTSLYEAATIEDIYLAYEQLKVDEDSLYSCIGVAGTQAGEEINAPLEVEETDDGLQGGIPVGRGLSTMTRPTT

Target ORF information:

RefSeq Version	XM_024595353.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595353.1

ORF Insert Sequence:

ATGGCGAGTA CAGCTGCCCC AAGCTCTGTA TTGATGACGA GCTTGCCTCA AATCCGTACA 
GATGATACTT TCTCAAGGAA GAACCGGATA AACCTAATTC GTTCCCCTTT GATTCGATTC 
CAAGCCAGCC ATCATCAATC TTGTCATTGC ATTTCCTCCA AAAGGTGGAA ACATGAGTTT 
GCTGCCGGTA GATCAGGTAC TAGTAGAAAG AAGAATAATG CTTGGAAACA TTGTAGTTCG 
TTCCTTGGTG AGAGAGTGGT TGAGGCATCT TTTCCAGAAC AATTTAAGTG CATGTCTTGC 
TCCCTTAACC GCCGTAGAAG CCGCTATAGC ATCAAGAGAT CTATACCTAG AGCCTTTATT 
GATAAGTCTG CTTTTCGTTT GTCTGGCCAT TCATTCGATA CTGCTTCTGC AAAGCATGTT 
CATGTCCCAT GTGCATCAAT GGGTCCAAAT GAGCCACATG CAGCAAGTAT AGCCTGCCCA 
GATGGTATTC TTGAGAGACA AGATTCCCAT TTATTAGATT CTGAATTAGA AAGAGCAAGA 
CTACTTGAGT TTTTGAATTC TGAACTTCCA TGTCACCCCA AGTTACACAG AGGACAACTG 
AAAAATGGAC TCTGTTATCT GATTCTACCA AATAAAGTTC CACCAAACAG CTTTCAATTT 
TTTAGGTTTG AGGCACACAT GGAAGTTCAT GTAGGATCAA TAGACGAGGA AGATGATGAG 
CAAGGAATTG CACATATGAT TGAACATGTT GCATTCCTTG GAAGTAAGAA ACGTGAGAAA 
CTTCTTGGAA CAGGGGCCCG ATCTAATGCT TACACTGATT TCCACCATAC AGTGTTCCAC 
ATTCATTCAC CAACTTGTAC AAAGGATGCT GATGGAGATC TACTCCCCTC TGTGTTGGAT 
GCCTTGAATG AGATAGCTTT CCACCCGAGC TTCCTCGCTT CTCGAGTTGA AAAGGAAAGG 
CGTGCTATAC TTTCAGAACT ACAGATGATG AATACTATAG AATATCGTGT TGACTGCCAG 
TTGTTACAAC ATCTACATTC AGAAAACAAG CTGAGCAAAA GGTTCCCAAT AGGATTGGAA 
GAGCAGATTA AGAAGTGGGA TGCAGAGAAA ATTAGGAAAT TCCATGAGCG TTGGTACTTC 
CCAGCAAATG CAACTCTATA CATTGTTGGT GACATTGATA ACATTTCAAA GACGGTTCAC 
CAAATTGAAA ATGTCTTTGG ACAAACTGGC CTGGAAAACA AGACAGTTTC TGCTCCTTCT 
CCAAGTGCAT TTGGTGCAAT GGCTAGTTTT CTTGCTCCTA AGGTCTCAGT TGGGCTCCCT 
GGAAGTTCAT CTCGTGAAAA GTCATCTAGT TCTCTAGACC AATCCAAAAT CATTAAAAGG 
GAAAGGCATG CAGTCCGTCC TCCTGTCGAG CATTATTGGT CTCTTCCTGG AAGCAATGCA 
AATTTGAAGC CACCACAGAT ATTTCAGCAT GAGTTTCTTC AGAATTTCTC AATTAATATG 
TTCTGCAAGA TTCCGGTGAG CAAGGTCCAA ACGAATGGTG ACTTGTGCAG TGTTTTGATG 
AAGAGAATAT TTCTATCTGC ACTGCACTTC CGAATTAATA CAAGATACAA GAGTTCAAAT 
CCACCATTCA CTTCAGTTGA ATTGGATCAT AGTGATTCTG GAAGGGAAGG ATGCACTGTC 
ACAACTCTTA CAGTGACTGC AGAACCCAAG AATTGGCAAA ATGCAATTAA AGTTGCTGTT 
CAGGAGGTTC GAAGGCTTAA AGAATTTGGT GTCACAAAGG GTGAATTAAC TCGCTATATG 
GATGCACTTT TAAAAGATAG CGAACATCTG GCAGCTATGA TTGATAATGT ATCATCTGTT 
GATAATTTGG AATTTATCAT GGAGAGTGAT GCTCTTGGCC ATACTGTGAT GGATCAGAGA 
CAAGGACATG AGAGTTTGTT TGCTGTTGCT GGAATGGTCA CACTTGAAGA AGTCAATTCT 
ATTGGTGCCA AGTTATTAGA ATTTATCTCT GATTTTGGAA AACCTACTGC GCCCATTCCT 
GCAGCAATCG TTGCATGTGT TCCTACAAAA GTGCATATTG ATGGATTGGG TGAAACCGAG 
TTCAAGATAT CCTCAAGTGA GATTACAGCT GCCATAAAAT CAGGATTGGA GGAAGCAATT 
GAGGCTGAGC CAGAGCTTGA AGTGCCAAAA GAATTGATAT CTTCAACACA GTTAGAGGAG 
TTAAGGTTAG AACGTAGGCC ATCCTTTGTT CCCTTACTTC CAGATGCAGG CTATACGAAA 
TTGCATGACC AAGAAACAGG CATCACTCAG TGTCGTCTTT CAAATGGAAT TGCTGTAAAT 
TACAAGATAT CCAAAAGTGA ATCCCGGGGA GGTGTCATGC GGCTTATTGT GGGTGGGGGC 
CGAGCAGCTG AAAGTTCCGA GTCTAAAGGA GCTGTTGTTG TGGGTGTTCG AACTCTCAGT 
GAGGGTGGTC GTGTTGGCAG CTTTTCGAGA GAGCAGGTAG AACTTTTTTG TGTGAATCAC 
CTAATAAATT GCTCTTTGGA GTCAACTGAG GAATTTATTT GTATGGAGTT CCGCTTCACT 
CTGCGAGACA ATGGGATGCA AGCAGCATTT GAATTACTTC ATATGGTACT TGAGAATAGT 
GTCTGGCTGG ATGATGCATT TGACAGAGCA AGGCAACTCT ACTTGTCATA TTACCGGTCC 
ATTCCCAAAA GCTTAGAACG GGCAACTGCT CACAAGCTCA TGACAGCAAT GTTGAATGGG 
GATGAGCGGT TCATTGAGCC AACCCCACAA TCATTACAAA ATTTAACACT GAAATCCGTA 
AAAGATGCAG TGATGAATCA GTTTGTTGGT GGAAACATGG AGGTAAGTAT TGTTGGAGAT 
TTCTCAGAGG AGGAGGTTCA GTCTTGCATT ATTGATTACC TGGGCACAGT TAGAGCAACA 
AGAGATTCTG ATCAGGAACA AGAATTTAAT CCTGTCATGT TTCGACCATC CCCTTCTGAT 
TTGCAGTTTC AACAAGTGTT TTTGAAGGAT ACTGATGAGA GAGCATGTGC ATATATTGCT 
GGTCCTGCAC CAAATCGTTG GGGTTTCACT GTTGATGGAA CAGACCTGTT CAAGTCAATG 
AGTGGCTTTT CGGTTTCAGC TGATGCACAA CCAATATCTG AAACACAACA GATAGACGGG 
ATGGATGTTC AGAAGGATAT GCAAGGAAAA CTTCGCTGTC ATCCACTCTT CTTTGGCATA 
ACAATGGGGC TACTGGCTGA GATCATAAAT TCTCGGCTTT TTACCACAGT TAGGGATTCT 
CTTGGGTTGA CATATGATGT ATCGTTTGAG TTAAGCCTAT TTGATAGGCT TAAGCTTGGT 
TGGTATGTTG TATCAGTAAC ATCAACTCCA GGCAAGGTAC ATAAAGCGGT TGATGCATGC 
AAAAGTGTTC TTAGAGGGTT GCATAGCAAC AAGGTTGCCC AGAGAGAGTT GGACAGGGCA 
AGACGGACGC TGCTAATGAG ACATGAAGCT GAGATTAAGT CCAATGCCTA TTGGCTTGGA 
TTGCTTGCTC ATTTGCAAGC CTCTTCTGTT CCGAGAAAGG ACGTCTCATG TATTAAAGAT 
CTCACTTCAC TCTATGAAGC TGCCACAATT GAGGACATAT ACCTTGCATA TGAACAATTG 
AAAGTAGATG AGGATTCTCT GTATTCATGC ATTGGGGTTG CTGGGACACA AGCTGGAGAG 
GAGATTAATG CTCCATTAGA AGTGGAAGAA ACAGATGATG GTTTGCAGGG AGGCATACCT 
GTGGGACGTG GTTTGTCTAC AATGACACGA CCAACAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7488593 ( XM_002301712.3 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002301748.3 Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPa424735
Clone ID Related Accession (Same CDS sequence)	XM_002301712.3
Accession Version	XM_002301712.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	stromal processing peptidase, chloroplastic isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 9, 2018 this sequence version replaced XM_002301712.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGTA CAGCTGCCCC AAGCTCTGTA TTGATGACGA GCTTGCCTCA AATCCGTACA 
GATGATACTT TCTCAAGGAA GAACCGGATA AACCTAATTC GTTCCCCTTT GATTCGATTC 
CAAGCCAGCC ATCATCAATC TTGTCATTGC ATTTCCTCCA AAAGGTGGAA ACATGAGTTT 
GCTGCCGGTA GATCAGGTAC TAGTAGAAAG AAGAATAATG CTTGGAAACA TTGTAGTTCG 
TTCCTTGGTG AGAGAGTGGT TGAGGCATCT TTTCCAGAAC AATTTAAGTG CATGTCTTGC 
TCCCTTAACC GCCGTAGAAG CCGCTATAGC ATCAAGAGAT CTATACCTAG AGCCTTTATT 
GATAAGTCTG CTTTTCGTTT GTCTGGCCAT TCATTCGATA CTGCTTCTGC AAAGCATGTT 
CATGTCCCAT GTGCATCAAT GGGTCCAAAT GAGCCACATG CAGCAAGTAT AGCCTGCCCA 
GATGGTATTC TTGAGAGACA AGATTCCCAT TTATTAGATT CTGAATTAGA AAGAGCAAGA 
CTACTTGAGT TTTTGAATTC TGAACTTCCA TGTCACCCCA AGTTACACAG AGGACAACTG 
AAAAATGGAC TCTGTTATCT GATTCTACCA AATAAAGTTC CACCAAACAG GTTTGAGGCA 
CACATGGAAG TTCATGTAGG ATCAATAGAC GAGGAAGATG ATGAGCAAGG AATTGCACAT 
ATGATTGAAC ATGTTGCATT CCTTGGAAGT AAGAAACGTG AGAAACTTCT TGGAACAGGG 
GCCCGATCTA ATGCTTACAC TGATTTCCAC CATACAGTGT TCCACATTCA TTCACCAACT 
TGTACAAAGG ATGCTGATGG AGATCTACTC CCCTCTGTGT TGGATGCCTT GAATGAGATA 
GCTTTCCACC CGAGCTTCCT CGCTTCTCGA GTTGAAAAGG AAAGGCGTGC TATACTTTCA 
GAACTACAGA TGATGAATAC TATAGAATAT CGTGTTGACT GCCAGTTGTT ACAACATCTA 
CATTCAGAAA ACAAGCTGAG CAAAAGGTTC CCAATAGGAT TGGAAGAGCA GATTAAGAAG 
TGGGATGCAG AGAAAATTAG GAAATTCCAT GAGCGTTGGT ACTTCCCAGC AAATGCAACT 
CTATACATTG TTGGTGACAT TGATAACATT TCAAAGACGG TTCACCAAAT TGAAAATGTC 
TTTGGACAAA CTGGCCTGGA AAACAAGACA GTTTCTGCTC CTTCTCCAAG TGCATTTGGT 
GCAATGGCTA GTTTTCTTGC TCCTAAGGTC TCAGTTGGGC TCCCTGGAAG TTCATCTCGT 
GAAAAGTCAT CTAGTTCTCT AGACCAATCC AAAATCATTA AAAGGGAAAG GCATGCAGTC 
CGTCCTCCTG TCGAGCATTA TTGGTCTCTT CCTGGAAGCA ATGCAAATTT GAAGCCACCA 
CAGATATTTC AGCATGAGTT TCTTCAGAAT TTCTCAATTA ATATGTTCTG CAAGATTCCG 
GTGAGCAAGG TCCAAACGAA TGGTGACTTG TGCAGTGTTT TGATGAAGAG AATATTTCTA 
TCTGCACTGC ACTTCCGAAT TAATACAAGA TACAAGAGTT CAAATCCACC ATTCACTTCA 
GTTGAATTGG ATCATAGTGA TTCTGGAAGG GAAGGATGCA CTGTCACAAC TCTTACAGTG 
ACTGCAGAAC CCAAGAATTG GCAAAATGCA ATTAAAGTTG CTGTTCAGGA GGTTCGAAGG 
CTTAAAGAAT TTGGTGTCAC AAAGGGTGAA TTAACTCGCT ATATGGATGC ACTTTTAAAA 
GATAGCGAAC ATCTGGCAGC TATGATTGAT AATGTATCAT CTGTTGATAA TTTGGAATTT 
ATCATGGAGA GTGATGCTCT TGGCCATACT GTGATGGATC AGAGACAAGG ACATGAGAGT 
TTGTTTGCTG TTGCTGGAAT GGTCACACTT GAAGAAGTCA ATTCTATTGG TGCCAAGTTA 
TTAGAATTTA TCTCTGATTT TGGAAAACCT ACTGCGCCCA TTCCTGCAGC AATCGTTGCA 
TGTGTTCCTA CAAAAGTGCA TATTGATGGA TTGGGTGAAA CCGAGTTCAA GATATCCTCA 
AGTGAGATTA CAGCTGCCAT AAAATCAGGA TTGGAGGAAG CAATTGAGGC TGAGCCAGAG 
CTTGAAGTGC CAAAAGAATT GATATCTTCA ACACAGTTAG AGGAGTTAAG GTTAGAACGT 
AGGCCATCCT TTGTTCCCTT ACTTCCAGAT GCAGGCTATA CGAAATTGCA TGACCAAGAA 
ACAGGCATCA CTCAGTGTCG TCTTTCAAAT GGAATTGCTG TAAATTACAA GATATCCAAA 
AGTGAATCCC GGGGAGGTGT CATGCGGCTT ATTGTGGGTG GGGGCCGAGC AGCTGAAAGT 
TCCGAGTCTA AAGGAGCTGT TGTTGTGGGT GTTCGAACTC TCAGTGAGGG TGGTCGTGTT 
GGCAGCTTTT CGAGAGAGCA GGTAGAACTT TTTTGTGTGA ATCACCTAAT AAATTGCTCT 
TTGGAGTCAA CTGAGGAATT TATTTGTATG GAGTTCCGCT TCACTCTGCG AGACAATGGG 
ATGCAAGCAG CATTTGAATT ACTTCATATG GTACTTGAGA ATAGTGTCTG GCTGGATGAT 
GCATTTGACA GAGCAAGGCA ACTCTACTTG TCATATTACC GGTCCATTCC CAAAAGCTTA 
GAACGGGCAA CTGCTCACAA GCTCATGACA GCAATGTTGA ATGGGGATGA GCGGTTCATT 
GAGCCAACCC CACAATCATT ACAAAATTTA ACACTGAAAT CCGTAAAAGA TGCAGTGATG 
AATCAGTTTG TTGGTGGAAA CATGGAGGTA AGTATTGTTG GAGATTTCTC AGAGGAGGAG 
GTTCAGTCTT GCATTATTGA TTACCTGGGC ACAGTTAGAG CAACAAGAGA TTCTGATCAG 
GAACAAGAAT TTAATCCTGT CATGTTTCGA CCATCCCCTT CTGATTTGCA GTTTCAACAA 
GTGTTTTTGA AGGATACTGA TGAGAGAGCA TGTGCATATA TTGCTGGTCC TGCACCAAAT 
CGTTGGGGTT TCACTGTTGA TGGAACAGAC CTGTTCAAGT CAATGAGTGG CTTTTCGGTT 
TCAGCTGATG CACAACCAAT ATCTGAAACA CAACAGATAG ACGGGATGGA TGTTCAGAAG 
GATATGCAAG GAAAACTTCG CTGTCATCCA CTCTTCTTTG GCATAACAAT GGGGCTACTG 
GCTGAGATCA TAAATTCTCG GCTTTTTACC ACAGTTAGGG ATTCTCTTGG GTTGACATAT 
GATGTATCGT TTGAGTTAAG CCTATTTGAT AGGCTTAAGC TTGGTTGGTA TGTTGTATCA 
GTAACATCAA CTCCAGGCAA GGTACATAAA GCGGTTGATG CATGCAAAAG TGTTCTTAGA 
GGGTTGCATA GCAACAAGGT TGCCCAGAGA GAGTTGGACA GGGCAAGACG GACGCTGCTA 
ATGAGACATG AAGCTGAGAT TAAGTCCAAT GCCTATTGGC TTGGATTGCT TGCTCATTTG 
CAAGCCTCTT CTGTTCCGAG AAAGGACGTC TCATGTATTA AAGATCTCAC TTCACTCTAT 
GAAGCTGCCA CAATTGAGGA CATATACCTT GCATATGAAC AATTGAAAGT AGATGAGGAT 
TCTCTGTATT CATGCATTGG GGTTGCTGGG ACACAAGCTG GAGAGGAGAT TAATGCTCCA 
TTAGAAGTGG AAGAAACAGA TGATGGTTTG CAGGGAGGCA TACCTGTGGG ACGTGGTTTG 
TCTACAATGA CACGACCAAC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002301748.3
CDS	212..4018
Translation	MASTAAPSSVLMTSLPQIRTDDTFSRKNRINLIRSPLIRFQASHHQSCHCISSKRWKHEFAAGRSGTSRKKNNAWKHCSSFLGERVVEASFPEQFKCMSCSLNRRRSRYSIKRSIPRAFIDKSAFRLSGHSFDTASAKHVHVPCASMGPNEPHAASIACPDGILERQDSHLLDSELERARLLEFLNSELPCHPKLHRGQLKNGLCYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTCTKDADGDLLPSVLDALNEIAFHPSFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKWDAEKIRKFHERWYFPANATLYIVGDIDNISKTVHQIENVFGQTGLENKTVSAPSPSAFGAMASFLAPKVSVGLPGSSSREKSSSSLDQSKIIKRERHAVRPPVEHYWSLPGSNANLKPPQIFQHEFLQNFSINMFCKIPVSKVQTNGDLCSVLMKRIFLSALHFRINTRYKSSNPPFTSVELDHSDSGREGCTVTTLTVTAEPKNWQNAIKVAVQEVRRLKEFGVTKGELTRYMDALLKDSEHLAAMIDNVSSVDNLEFIMESDALGHTVMDQRQGHESLFAVAGMVTLEEVNSIGAKLLEFISDFGKPTAPIPAAIVACVPTKVHIDGLGETEFKISSSEITAAIKSGLEEAIEAEPELEVPKELISSTQLEELRLERRPSFVPLLPDAGYTKLHDQETGITQCRLSNGIAVNYKISKSESRGGVMRLIVGGGRAAESSESKGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINCSLESTEEFICMEFRFTLRDNGMQAAFELLHMVLENSVWLDDAFDRARQLYLSYYRSIPKSLERATAHKLMTAMLNGDERFIEPTPQSLQNLTLKSVKDAVMNQFVGGNMEVSIVGDFSEEEVQSCIIDYLGTVRATRDSDQEQEFNPVMFRPSPSDLQFQQVFLKDTDERACAYIAGPAPNRWGFTVDGTDLFKSMSGFSVSADAQPISETQQIDGMDVQKDMQGKLRCHPLFFGITMGLLAEIINSRLFTTVRDSLGLTYDVSFELSLFDRLKLGWYVVSVTSTPGKVHKAVDACKSVLRGLHSNKVAQRELDRARRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDVSCIKDLTSLYEAATIEDIYLAYEQLKVDEDSLYSCIGVAGTQAGEEINAPLEVEETDDGLQGGIPVGRGLSTMTRPTT

Target ORF information:

RefSeq Version	XM_002301712.3
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002301712.3

ORF Insert Sequence:

ATGGCGAGTA CAGCTGCCCC AAGCTCTGTA TTGATGACGA GCTTGCCTCA AATCCGTACA 
GATGATACTT TCTCAAGGAA GAACCGGATA AACCTAATTC GTTCCCCTTT GATTCGATTC 
CAAGCCAGCC ATCATCAATC TTGTCATTGC ATTTCCTCCA AAAGGTGGAA ACATGAGTTT 
GCTGCCGGTA GATCAGGTAC TAGTAGAAAG AAGAATAATG CTTGGAAACA TTGTAGTTCG 
TTCCTTGGTG AGAGAGTGGT TGAGGCATCT TTTCCAGAAC AATTTAAGTG CATGTCTTGC 
TCCCTTAACC GCCGTAGAAG CCGCTATAGC ATCAAGAGAT CTATACCTAG AGCCTTTATT 
GATAAGTCTG CTTTTCGTTT GTCTGGCCAT TCATTCGATA CTGCTTCTGC AAAGCATGTT 
CATGTCCCAT GTGCATCAAT GGGTCCAAAT GAGCCACATG CAGCAAGTAT AGCCTGCCCA 
GATGGTATTC TTGAGAGACA AGATTCCCAT TTATTAGATT CTGAATTAGA AAGAGCAAGA 
CTACTTGAGT TTTTGAATTC TGAACTTCCA TGTCACCCCA AGTTACACAG AGGACAACTG 
AAAAATGGAC TCTGTTATCT GATTCTACCA AATAAAGTTC CACCAAACAG GTTTGAGGCA 
CACATGGAAG TTCATGTAGG ATCAATAGAC GAGGAAGATG ATGAGCAAGG AATTGCACAT 
ATGATTGAAC ATGTTGCATT CCTTGGAAGT AAGAAACGTG AGAAACTTCT TGGAACAGGG 
GCCCGATCTA ATGCTTACAC TGATTTCCAC CATACAGTGT TCCACATTCA TTCACCAACT 
TGTACAAAGG ATGCTGATGG AGATCTACTC CCCTCTGTGT TGGATGCCTT GAATGAGATA 
GCTTTCCACC CGAGCTTCCT CGCTTCTCGA GTTGAAAAGG AAAGGCGTGC TATACTTTCA 
GAACTACAGA TGATGAATAC TATAGAATAT CGTGTTGACT GCCAGTTGTT ACAACATCTA 
CATTCAGAAA ACAAGCTGAG CAAAAGGTTC CCAATAGGAT TGGAAGAGCA GATTAAGAAG 
TGGGATGCAG AGAAAATTAG GAAATTCCAT GAGCGTTGGT ACTTCCCAGC AAATGCAACT 
CTATACATTG TTGGTGACAT TGATAACATT TCAAAGACGG TTCACCAAAT TGAAAATGTC 
TTTGGACAAA CTGGCCTGGA AAACAAGACA GTTTCTGCTC CTTCTCCAAG TGCATTTGGT 
GCAATGGCTA GTTTTCTTGC TCCTAAGGTC TCAGTTGGGC TCCCTGGAAG TTCATCTCGT 
GAAAAGTCAT CTAGTTCTCT AGACCAATCC AAAATCATTA AAAGGGAAAG GCATGCAGTC 
CGTCCTCCTG TCGAGCATTA TTGGTCTCTT CCTGGAAGCA ATGCAAATTT GAAGCCACCA 
CAGATATTTC AGCATGAGTT TCTTCAGAAT TTCTCAATTA ATATGTTCTG CAAGATTCCG 
GTGAGCAAGG TCCAAACGAA TGGTGACTTG TGCAGTGTTT TGATGAAGAG AATATTTCTA 
TCTGCACTGC ACTTCCGAAT TAATACAAGA TACAAGAGTT CAAATCCACC ATTCACTTCA 
GTTGAATTGG ATCATAGTGA TTCTGGAAGG GAAGGATGCA CTGTCACAAC TCTTACAGTG 
ACTGCAGAAC CCAAGAATTG GCAAAATGCA ATTAAAGTTG CTGTTCAGGA GGTTCGAAGG 
CTTAAAGAAT TTGGTGTCAC AAAGGGTGAA TTAACTCGCT ATATGGATGC ACTTTTAAAA 
GATAGCGAAC ATCTGGCAGC TATGATTGAT AATGTATCAT CTGTTGATAA TTTGGAATTT 
ATCATGGAGA GTGATGCTCT TGGCCATACT GTGATGGATC AGAGACAAGG ACATGAGAGT 
TTGTTTGCTG TTGCTGGAAT GGTCACACTT GAAGAAGTCA ATTCTATTGG TGCCAAGTTA 
TTAGAATTTA TCTCTGATTT TGGAAAACCT ACTGCGCCCA TTCCTGCAGC AATCGTTGCA 
TGTGTTCCTA CAAAAGTGCA TATTGATGGA TTGGGTGAAA CCGAGTTCAA GATATCCTCA 
AGTGAGATTA CAGCTGCCAT AAAATCAGGA TTGGAGGAAG CAATTGAGGC TGAGCCAGAG 
CTTGAAGTGC CAAAAGAATT GATATCTTCA ACACAGTTAG AGGAGTTAAG GTTAGAACGT 
AGGCCATCCT TTGTTCCCTT ACTTCCAGAT GCAGGCTATA CGAAATTGCA TGACCAAGAA 
ACAGGCATCA CTCAGTGTCG TCTTTCAAAT GGAATTGCTG TAAATTACAA GATATCCAAA 
AGTGAATCCC GGGGAGGTGT CATGCGGCTT ATTGTGGGTG GGGGCCGAGC AGCTGAAAGT 
TCCGAGTCTA AAGGAGCTGT TGTTGTGGGT GTTCGAACTC TCAGTGAGGG TGGTCGTGTT 
GGCAGCTTTT CGAGAGAGCA GGTAGAACTT TTTTGTGTGA ATCACCTAAT AAATTGCTCT 
TTGGAGTCAA CTGAGGAATT TATTTGTATG GAGTTCCGCT TCACTCTGCG AGACAATGGG 
ATGCAAGCAG CATTTGAATT ACTTCATATG GTACTTGAGA ATAGTGTCTG GCTGGATGAT 
GCATTTGACA GAGCAAGGCA ACTCTACTTG TCATATTACC GGTCCATTCC CAAAAGCTTA 
GAACGGGCAA CTGCTCACAA GCTCATGACA GCAATGTTGA ATGGGGATGA GCGGTTCATT 
GAGCCAACCC CACAATCATT ACAAAATTTA ACACTGAAAT CCGTAAAAGA TGCAGTGATG 
AATCAGTTTG TTGGTGGAAA CATGGAGGTA AGTATTGTTG GAGATTTCTC AGAGGAGGAG 
GTTCAGTCTT GCATTATTGA TTACCTGGGC ACAGTTAGAG CAACAAGAGA TTCTGATCAG 
GAACAAGAAT TTAATCCTGT CATGTTTCGA CCATCCCCTT CTGATTTGCA GTTTCAACAA 
GTGTTTTTGA AGGATACTGA TGAGAGAGCA TGTGCATATA TTGCTGGTCC TGCACCAAAT 
CGTTGGGGTT TCACTGTTGA TGGAACAGAC CTGTTCAAGT CAATGAGTGG CTTTTCGGTT 
TCAGCTGATG CACAACCAAT ATCTGAAACA CAACAGATAG ACGGGATGGA TGTTCAGAAG 
GATATGCAAG GAAAACTTCG CTGTCATCCA CTCTTCTTTG GCATAACAAT GGGGCTACTG 
GCTGAGATCA TAAATTCTCG GCTTTTTACC ACAGTTAGGG ATTCTCTTGG GTTGACATAT 
GATGTATCGT TTGAGTTAAG CCTATTTGAT AGGCTTAAGC TTGGTTGGTA TGTTGTATCA 
GTAACATCAA CTCCAGGCAA GGTACATAAA GCGGTTGATG CATGCAAAAG TGTTCTTAGA 
GGGTTGCATA GCAACAAGGT TGCCCAGAGA GAGTTGGACA GGGCAAGACG GACGCTGCTA 
ATGAGACATG AAGCTGAGAT TAAGTCCAAT GCCTATTGGC TTGGATTGCT TGCTCATTTG 
CAAGCCTCTT CTGTTCCGAG AAAGGACGTC TCATGTATTA AAGATCTCAC TTCACTCTAT 
GAAGCTGCCA CAATTGAGGA CATATACCTT GCATATGAAC AATTGAAAGT AGATGAGGAT 
TCTCTGTATT CATGCATTGG GGTTGCTGGG ACACAAGCTG GAGAGGAGAT TAATGCTCCA 
TTAGAAGTGG AAGAAACAGA TGATGGTTTG CAGGGAGGCA TACCTGTGGG ACGTGGTTTG 
TCTACAATGA CACGACCAAC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7488593 ( XM_024595354.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451122.1 Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X6, mRNA.

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CloneID	OPa424736
Clone ID Related Accession (Same CDS sequence)	XM_024595354.1
Accession Version	XM_024595354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	stromal processing peptidase, chloroplastic isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCAA ATGAGCCACA TGCAGCAAGT ATAGCCTGCC CAGATGGTAT TCTTGAGAGA 
CAAGATTCCC ATTTATTAGA TTCTGAATTA GAAAGAGCAA GACTACTTGA GTTTTTGAAT 
TCTGAACTTC CATGTCACCC CAAGTTACAC AGAGGACAAC TGAAAAATGG ACTCTGTTAT 
CTGATTCTAC CAAATAAAGT TCCACCAAAC AGCTTTCAAT TTTTTAGGTT TGAGGCACAC 
ATGGAAGTTC ATGTAGGATC AATAGACGAG GAAGATGATG AGCAAGGAAT TGCACATATG 
ATTGAACATG TTGCATTCCT TGGAAGTAAG AAACGTGAGA AACTTCTTGG AACAGGGGCC 
CGATCTAATG CTTACACTGA TTTCCACCAT ACAGTGTTCC ACATTCATTC ACCAACTTGT 
ACAAAGGATG CTGATGGAGA TCTACTCCCC TCTGTGTTGG ATGCCTTGAA TGAGATAGCT 
TTCCACCCGA GCTTCCTCGC TTCTCGAGTT GAAAAGGAAA GGCGTGCTAT ACTTTCAGAA 
CTACAGATGA TGAATACTAT AGAATATCGT GTTGACTGCC AGTTGTTACA ACATCTACAT 
TCAGAAAACA AGCTGAGCAA AAGGTTCCCA ATAGGATTGG AAGAGCAGAT TAAGAAGTGG 
GATGCAGAGA AAATTAGGAA ATTCCATGAG CGTTGGTACT TCCCAGCAAA TGCAACTCTA 
TACATTGTTG GTGACATTGA TAACATTTCA AAGACGGTTC ACCAAATTGA AAATGTCTTT 
GGACAAACTG GCCTGGAAAA CAAGACAGTT TCTGCTCCTT CTCCAAGTGC ATTTGGTGCA 
ATGGCTAGTT TTCTTGCTCC TAAGGTCTCA GTTGGGCTCC CTGGAAGTTC ATCTCGTGAA 
AAGTCATCTA GTTCTCTAGA CCAATCCAAA ATCATTAAAA GGGAAAGGCA TGCAGTCCGT 
CCTCCTGTCG AGCATTATTG GTCTCTTCCT GGAAGCAATG CAAATTTGAA GCCACCACAG 
ATATTTCAGC ATGAGTTTCT TCAGAATTTC TCAATTAATA TGTTCTGCAA GATTCCGGTG 
AGCAAGGTCC AAACGAATGG TGACTTGTGC AGTGTTTTGA TGAAGAGAAT ATTTCTATCT 
GCACTGCACT TCCGAATTAA TACAAGATAC AAGAGTTCAA ATCCACCATT CACTTCAGTT 
GAATTGGATC ATAGTGATTC TGGAAGGGAA GGATGCACTG TCACAACTCT TACAGTGACT 
GCAGAACCCA AGAATTGGCA AAATGCAATT AAAGTTGCTG TTCAGGAGGT TCGAAGGCTT 
AAAGAATTTG GTGTCACAAA GGGTGAATTA ACTCGCTATA TGGATGCACT TTTAAAAGAT 
AGCGAACATC TGGCAGCTAT GATTGATAAT GTATCATCTG TTGATAATTT GGAATTTATC 
ATGGAGAGTG ATGCTCTTGG CCATACTGTG ATGGATCAGA GACAAGGACA TGAGAGTTTG 
TTTGCTGTTG CTGGAATGGT CACACTTGAA GAAGTCAATT CTATTGGTGC CAAGTTATTA 
GAATTTATCT CTGATTTTGG AAAACCTACT GCGCCCATTC CTGCAGCAAT CGTTGCATGT 
GTTCCTACAA AAGTGCATAT TGATGGATTG GGTGAAACCG AGTTCAAGAT ATCCTCAAGT 
GAGATTACAG CTGCCATAAA ATCAGGATTG GAGGAAGCAA TTGAGGCTGA GCCAGAGCTT 
GAAGTGCCAA AAGAATTGAT ATCTTCAACA CAGTTAGAGG AGTTAAGGTT AGAACGTAGG 
CCATCCTTTG TTCCCTTACT TCCAGATGCA GGCTATACGA AATTGCATGA CCAAGAAACA 
GGCATCACTC AGTGTCGTCT TTCAAATGGA ATTGCTGTAA ATTACAAGAT ATCCAAAAGT 
GAATCCCGGG GAGGTGTCAT GCGGCTTATT GTGGGTGGGG GCCGAGCAGC TGAAAGTTCC 
GAGTCTAAAG GAGCTGTTGT TGTGGGTGTT CGAACTCTCA GTGAGGGTGG TCGTGTTGGC 
AGCTTTTCGA GAGAGCAGGT AGAACTTTTT TGTGTGAATC ACCTAATAAA TTGCTCTTTG 
GAGTCAACTG AGGAATTTAT TTGTATGGAG TTCCGCTTCA CTCTGCGAGA CAATGGGATG 
CAAGCAGCAT TTGAATTACT TCATATGGTA CTTGAGAATA GTGTCTGGCT GGATGATGCA 
TTTGACAGAG CAAGGCAACT CTACTTGTCA TATTACCGGT CCATTCCCAA AAGCTTAGAA 
CGGGCAACTG CTCACAAGCT CATGACAGCA ATGTTGAATG GGGATGAGCG GTTCATTGAG 
CCAACCCCAC AATCATTACA AAATTTAACA CTGAAATCCG TAAAAGATGC AGTGATGAAT 
CAGTTTGTTG GTGGAAACAT GGAGGTAAGT ATTGTTGGAG ATTTCTCAGA GGAGGAGGTT 
CAGTCTTGCA TTATTGATTA CCTGGGCACA GTTAGAGCAA CAAGAGATTC TGATCAGGAA 
CAAGAATTTA ATCCTGTCAT GTTTCGACCA TCCCCTTCTG ATTTGCAGTT TCAACAAGTG 
TTTTTGAAGG ATACTGATGA GAGAGCATGT GCATATATTG CTGGTCCTGC ACCAAATCGT 
TGGGGTTTCA CTGTTGATGG AACAGACCTG TTCAAGTCAA TGAGTGGCTT TTCGGTTTCA 
GCTGCTGGCC AATGCTTTGC AGATGCACAA CCAATATCTG AAACACAACA GATAGACGGG 
ATGGATGTTC AGAAGGATAT GCAAGGAAAA CTTCGCTGTC ATCCACTCTT CTTTGGCATA 
ACAATGGGGC TACTGGCTGA GATCATAAAT TCTCGGCTTT TTACCACAGT TAGGGATTCT 
CTTGGGTTGA CATATGATGT ATCGTTTGAG TTAAGCCTAT TTGATAGGCT TAAGCTTGGT 
TGGTATGTTG TATCAGTAAC ATCAACTCCA GGCAAGGTAC ATAAAGCGGT TGATGCATGC 
AAAAGTGTTC TTAGAGGGTT GCATAGCAAC AAGGTTGCCC AGAGAGAGTT GGACAGGGCA 
AGACGGACGC TGCTAATGAG ACATGAAGCT GAGATTAAGT CCAATGCCTA TTGGCTTGGA 
TTGCTTGCTC ATTTGCAAGC CTCTTCTGTT CCGAGAAAGG ACGTCTCATG TATTAAAGAT 
CTCACTTCAC TCTATGAAGC TGCCACAATT GAGGACATAT ACCTTGCATA TGAACAATTG 
AAAGTAGATG AGGATTCTCT GTATTCATGC ATTGGGGTTG CTGGGACACA AGCTGGAGAG 
GAGATTAATG CTCCATTAGA AGTGGAAGAA ACAGATGATG GTTTGCAGGG AGGCATACCT 
GTGGGACGTG GTTTGTCTAC AATGACACGA CCAACAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451122.1
CDS	166..3567
Translation	MGPNEPHAASIACPDGILERQDSHLLDSELERARLLEFLNSELPCHPKLHRGQLKNGLCYLILPNKVPPNSFQFFRFEAHMEVHVGSIDEEDDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTCTKDADGDLLPSVLDALNEIAFHPSFLASRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKWDAEKIRKFHERWYFPANATLYIVGDIDNISKTVHQIENVFGQTGLENKTVSAPSPSAFGAMASFLAPKVSVGLPGSSSREKSSSSLDQSKIIKRERHAVRPPVEHYWSLPGSNANLKPPQIFQHEFLQNFSINMFCKIPVSKVQTNGDLCSVLMKRIFLSALHFRINTRYKSSNPPFTSVELDHSDSGREGCTVTTLTVTAEPKNWQNAIKVAVQEVRRLKEFGVTKGELTRYMDALLKDSEHLAAMIDNVSSVDNLEFIMESDALGHTVMDQRQGHESLFAVAGMVTLEEVNSIGAKLLEFISDFGKPTAPIPAAIVACVPTKVHIDGLGETEFKISSSEITAAIKSGLEEAIEAEPELEVPKELISSTQLEELRLERRPSFVPLLPDAGYTKLHDQETGITQCRLSNGIAVNYKISKSESRGGVMRLIVGGGRAAESSESKGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINCSLESTEEFICMEFRFTLRDNGMQAAFELLHMVLENSVWLDDAFDRARQLYLSYYRSIPKSLERATAHKLMTAMLNGDERFIEPTPQSLQNLTLKSVKDAVMNQFVGGNMEVSIVGDFSEEEVQSCIIDYLGTVRATRDSDQEQEFNPVMFRPSPSDLQFQQVFLKDTDERACAYIAGPAPNRWGFTVDGTDLFKSMSGFSVSAAGQCFADAQPISETQQIDGMDVQKDMQGKLRCHPLFFGITMGLLAEIINSRLFTTVRDSLGLTYDVSFELSLFDRLKLGWYVVSVTSTPGKVHKAVDACKSVLRGLHSNKVAQRELDRARRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDVSCIKDLTSLYEAATIEDIYLAYEQLKVDEDSLYSCIGVAGTQAGEEINAPLEVEETDDGLQGGIPVGRGLSTMTRPTT

Target ORF information:

RefSeq Version	XM_024595354.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa stromal processing peptidase, chloroplastic (LOC7488593), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595354.1

ORF Insert Sequence:

ATGGGTCCAA ATGAGCCACA TGCAGCAAGT ATAGCCTGCC CAGATGGTAT TCTTGAGAGA 
CAAGATTCCC ATTTATTAGA TTCTGAATTA GAAAGAGCAA GACTACTTGA GTTTTTGAAT 
TCTGAACTTC CATGTCACCC CAAGTTACAC AGAGGACAAC TGAAAAATGG ACTCTGTTAT 
CTGATTCTAC CAAATAAAGT TCCACCAAAC AGCTTTCAAT TTTTTAGGTT TGAGGCACAC 
ATGGAAGTTC ATGTAGGATC AATAGACGAG GAAGATGATG AGCAAGGAAT TGCACATATG 
ATTGAACATG TTGCATTCCT TGGAAGTAAG AAACGTGAGA AACTTCTTGG AACAGGGGCC 
CGATCTAATG CTTACACTGA TTTCCACCAT ACAGTGTTCC ACATTCATTC ACCAACTTGT 
ACAAAGGATG CTGATGGAGA TCTACTCCCC TCTGTGTTGG ATGCCTTGAA TGAGATAGCT 
TTCCACCCGA GCTTCCTCGC TTCTCGAGTT GAAAAGGAAA GGCGTGCTAT ACTTTCAGAA 
CTACAGATGA TGAATACTAT AGAATATCGT GTTGACTGCC AGTTGTTACA ACATCTACAT 
TCAGAAAACA AGCTGAGCAA AAGGTTCCCA ATAGGATTGG AAGAGCAGAT TAAGAAGTGG 
GATGCAGAGA AAATTAGGAA ATTCCATGAG CGTTGGTACT TCCCAGCAAA TGCAACTCTA 
TACATTGTTG GTGACATTGA TAACATTTCA AAGACGGTTC ACCAAATTGA AAATGTCTTT 
GGACAAACTG GCCTGGAAAA CAAGACAGTT TCTGCTCCTT CTCCAAGTGC ATTTGGTGCA 
ATGGCTAGTT TTCTTGCTCC TAAGGTCTCA GTTGGGCTCC CTGGAAGTTC ATCTCGTGAA 
AAGTCATCTA GTTCTCTAGA CCAATCCAAA ATCATTAAAA GGGAAAGGCA TGCAGTCCGT 
CCTCCTGTCG AGCATTATTG GTCTCTTCCT GGAAGCAATG CAAATTTGAA GCCACCACAG 
ATATTTCAGC ATGAGTTTCT TCAGAATTTC TCAATTAATA TGTTCTGCAA GATTCCGGTG 
AGCAAGGTCC AAACGAATGG TGACTTGTGC AGTGTTTTGA TGAAGAGAAT ATTTCTATCT 
GCACTGCACT TCCGAATTAA TACAAGATAC AAGAGTTCAA ATCCACCATT CACTTCAGTT 
GAATTGGATC ATAGTGATTC TGGAAGGGAA GGATGCACTG TCACAACTCT TACAGTGACT 
GCAGAACCCA AGAATTGGCA AAATGCAATT AAAGTTGCTG TTCAGGAGGT TCGAAGGCTT 
AAAGAATTTG GTGTCACAAA GGGTGAATTA ACTCGCTATA TGGATGCACT TTTAAAAGAT 
AGCGAACATC TGGCAGCTAT GATTGATAAT GTATCATCTG TTGATAATTT GGAATTTATC 
ATGGAGAGTG ATGCTCTTGG CCATACTGTG ATGGATCAGA GACAAGGACA TGAGAGTTTG 
TTTGCTGTTG CTGGAATGGT CACACTTGAA GAAGTCAATT CTATTGGTGC CAAGTTATTA 
GAATTTATCT CTGATTTTGG AAAACCTACT GCGCCCATTC CTGCAGCAAT CGTTGCATGT 
GTTCCTACAA AAGTGCATAT TGATGGATTG GGTGAAACCG AGTTCAAGAT ATCCTCAAGT 
GAGATTACAG CTGCCATAAA ATCAGGATTG GAGGAAGCAA TTGAGGCTGA GCCAGAGCTT 
GAAGTGCCAA AAGAATTGAT ATCTTCAACA CAGTTAGAGG AGTTAAGGTT AGAACGTAGG 
CCATCCTTTG TTCCCTTACT TCCAGATGCA GGCTATACGA AATTGCATGA CCAAGAAACA 
GGCATCACTC AGTGTCGTCT TTCAAATGGA ATTGCTGTAA ATTACAAGAT ATCCAAAAGT 
GAATCCCGGG GAGGTGTCAT GCGGCTTATT GTGGGTGGGG GCCGAGCAGC TGAAAGTTCC 
GAGTCTAAAG GAGCTGTTGT TGTGGGTGTT CGAACTCTCA GTGAGGGTGG TCGTGTTGGC 
AGCTTTTCGA GAGAGCAGGT AGAACTTTTT TGTGTGAATC ACCTAATAAA TTGCTCTTTG 
GAGTCAACTG AGGAATTTAT TTGTATGGAG TTCCGCTTCA CTCTGCGAGA CAATGGGATG 
CAAGCAGCAT TTGAATTACT TCATATGGTA CTTGAGAATA GTGTCTGGCT GGATGATGCA 
TTTGACAGAG CAAGGCAACT CTACTTGTCA TATTACCGGT CCATTCCCAA AAGCTTAGAA 
CGGGCAACTG CTCACAAGCT CATGACAGCA ATGTTGAATG GGGATGAGCG GTTCATTGAG 
CCAACCCCAC AATCATTACA AAATTTAACA CTGAAATCCG TAAAAGATGC AGTGATGAAT 
CAGTTTGTTG GTGGAAACAT GGAGGTAAGT ATTGTTGGAG ATTTCTCAGA GGAGGAGGTT 
CAGTCTTGCA TTATTGATTA CCTGGGCACA GTTAGAGCAA CAAGAGATTC TGATCAGGAA 
CAAGAATTTA ATCCTGTCAT GTTTCGACCA TCCCCTTCTG ATTTGCAGTT TCAACAAGTG 
TTTTTGAAGG ATACTGATGA GAGAGCATGT GCATATATTG CTGGTCCTGC ACCAAATCGT 
TGGGGTTTCA CTGTTGATGG AACAGACCTG TTCAAGTCAA TGAGTGGCTT TTCGGTTTCA 
GCTGCTGGCC AATGCTTTGC AGATGCACAA CCAATATCTG AAACACAACA GATAGACGGG 
ATGGATGTTC AGAAGGATAT GCAAGGAAAA CTTCGCTGTC ATCCACTCTT CTTTGGCATA 
ACAATGGGGC TACTGGCTGA GATCATAAAT TCTCGGCTTT TTACCACAGT TAGGGATTCT 
CTTGGGTTGA CATATGATGT ATCGTTTGAG TTAAGCCTAT TTGATAGGCT TAAGCTTGGT 
TGGTATGTTG TATCAGTAAC ATCAACTCCA GGCAAGGTAC ATAAAGCGGT TGATGCATGC 
AAAAGTGTTC TTAGAGGGTT GCATAGCAAC AAGGTTGCCC AGAGAGAGTT GGACAGGGCA 
AGACGGACGC TGCTAATGAG ACATGAAGCT GAGATTAAGT CCAATGCCTA TTGGCTTGGA 
TTGCTTGCTC ATTTGCAAGC CTCTTCTGTT CCGAGAAAGG ACGTCTCATG TATTAAAGAT 
CTCACTTCAC TCTATGAAGC TGCCACAATT GAGGACATAT ACCTTGCATA TGAACAATTG 
AAAGTAGATG AGGATTCTCT GTATTCATGC ATTGGGGTTG CTGGGACACA AGCTGGAGAG 
GAGATTAATG CTCCATTAGA AGTGGAAGAA ACAGATGATG GTTTGCAGGG AGGCATACCT 
GTGGGACGTG GTTTGTCTAC AATGACACGA CCAACAACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7488593 ( XM_002301712.2 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002301748.2 Populus trichocarpa hypothetical protein (POPTR_0002s23680g) mRNA, complete cds.

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CloneID	OPa06350
Clone ID Related Accession (Same CDS sequence)	XM_002301712.2
Accession Version	XM_002301712.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-12-30
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008468). On Dec 16, 2013 this sequence version replaced XM_002301712.1.

ATGGCGAGTA CAGCTGCCCC AAGCTCTGTA TTGATGACGA GCTTGCCTCA AATCCGTACA 
GATGATACTT TCTCAAGGAA GAACCGGATA AACCTAATTC GTTCCCCTTT GATTCGATTC 
CAAGCCAGCC ATCATCAATC TTGTCATTGC ATTTCCTCCA AAAGGTGGAA ACATGAGTTT 
GCTGCCGGTA GATCAGGTAC TAGTAGAAAG AAGAATAATG CTTGGAAACA TTGTAGTTCG 
TTCCTTGGTG AGAGAGTGGT TGAGGCATCT TTTCCAGAAC AATTTAAGTG CATGTCTTGC 
TCCCTTAACC GCCGTAGAAG CCGCTATAGC ATCAAGAGAT CTATACCTAG AGCCTTTATT 
GATAAGTCTG CTTTTCGTTT GTCTGGCCAT TCATTCGATA CTGCTTCTGC AAAGCATGTT 
CATGTCCCAT GTGCATCAAT GGGTCCAAAT GAGCCACATG CAGCAAGTAT AGCCTGCCCA 
GATGGTATTC TTGAGAGACA AGATTCCCAT TTATTAGATT CTGAATTAGA AAGAGCAAGA 
CTACTTGAGT TTTTGAATTC TGAACTTCCA TGTCACCCCA AGTTACACAG AGGACAACTG 
AAAAATGGAC TCTGTTATCT GATTCTACCA AATAAAGTTC CACCAAACAG GTTTGAGGCA 
CACATGGAAG TTCATGTAGG ATCAATAGAC GAGGAAGATG ATGAGCAAGG AATTGCACAT 
ATGATTGAAC ATGTTGCATT CCTTGGAAGT AAGAAACGTG AGAAACTTCT TGGAACAGGG 
GCCCGATCTA ATGCTTACAC TGATTTCCAC CATACAGTGT TCCACATTCA TTCACCAACT 
TGTACAAAGG ATGCTGATGG AGATCTACTC CCCTCTGTGT TGGATGCCTT GAATGAGATA 
GCTTTCCACC CGAGCTTCCT CGCTTCTCGA GTTGAAAAGG AAAGGCGTGC TATACTTTCA 
GAACTACGGA TGATGAATAC TATAGAATAT CGTGTTGACT GCCAGTTGTT ACAACATCTA 
CATTCAGAAA ACAAGCTGAG CAAAAGGTTC CCAATAGGAT TGGAAGAGCA GATTAAGAAG 
TGGGATGCAG AGAAAATTAG GAAATTCCAT GAGCGTTGGT ACTTCCCAGC AAATGCAACT 
CTATACATTG TTGGTGACAT TGATAACATT TCAAAGACGG TTCACCAAAT TGAAAATGTC 
TTTGGACAAA CTGGCCTGGA AAACAAGACA GTTTCTGCTC CTTCTCCAAG TGCATTTGGT 
GCAATGGCTA GTTTTCTTGC TCCTAAGGTC TCAGTTGGGC TCCCTGGAAG TTCATCTCGT 
GAAAAGTCAT CTAGTTCTCT AGACCAATCC AAAATCATTA AAAGGGAAAG GCATGCAGTC 
CGTCCTCCTG TCGAGCATTA TTGGTCTCTT CCTGGAAGCA ATGCAAATTT GAAGCCACCA 
CAGATATTTC AGCATGAGTT TCTTCAGAAT TTCTCAATTA ATATGTTCTG CAAGATTCCG 
GTGAGCAAGG TCCAAACGAA TGGTGACTTG TGCAGTGTTT TGATGAAGAG AATATTTCTA 
TCTGCACTGC ACTTCCGAAT TAATACAAGA TACAAGAGTT CAAATCCACC ATTCACTTCA 
GTTGAATTGG ATCATAGTGA TTCTGGAAGG GAAGGATGCA CTGTCACAAC TCTTACAGTG 
ACTGCAGAAC CCAAGAATTG GCAAAATGCA ATTAAAGTTG CTGTTCAGGA GGTTCGAAGG 
CTTAAAGAAT TTGGTGTCAC AAAGGGTGAA TTAACTCGCT ATATGGATGC ACTTTTAAAA 
GATAGCGAAC ATCTGGCAGC TATGATTGAT AATGTATCAT CTGTTGATAA TTTGGAATTT 
ATCATGGAGA GTGATGCTCT TGGCCATACT GTGATGGATC AGAGACAAGG ACATGAGAGT 
TTGTTTGCTG TTGCTGGAAT GGTCACACTT GAAGAAGTCA ATTCTATTGG TGCCAAGTTA 
TTAGAATTTA TCTCTGATTT TGGAAAACCT ACTGCGCCCA TTCCTGCAGC AATCGTTGCA 
TGTGTTCCTA CAAAAGTGCA TATTGATGGA TTGGGTGAAA CCGAGTTCAA GATATCCTCA 
AGTGAGATTA CAGCTGCCAT AAAATCAGGA TTGGAGGAAG CAATTGAGGC TGAGCCAGAG 
CTTGAAGTGC CAAAAGAATT GATATCTTCA ACACAGTTAG AGGAGTTAAG GTTAGAACGT 
AGGCCATCCT TTGTTCCCTT ACTTCCAGAT GCAGGCTATA CGAAATTGCA TGACCAAGAA 
ACAGGCATCA CTCAGTGTCG TCTTTCAAAT GGAATTGCTG TAAATTACAA GATATCCAAA 
AGTGAATCCC GGGGAGGTGT CATGCGGCTT ATTGTGGGTG GGGGCCGAGC AGCTGAAAGT 
TCCGAGTCTA AAGGAGCTGT TGTTGTGGGT GTTCGAACTC TCAGTGAGGG TGGTCGTGTT 
GGCAGCTTTT CGAGAGAGCA GGTAGAACTT TTTTGTGTGA ATCACCTAAT AAATTGCTCT 
TTGGAGTCAA CTGAGGAATT TATTTGTATG GAGTTCCGCT TCACTCTGCG AGACAATGGG 
ATGCAAGCAG CATTTGAATT ACTTCATATG GTACTTGAGA ATAGTGTCTG GCTGGATGAT 
GCATTTGACA GAGCAAGGCA ACTCTACTTG TCATATTACC GGTCCATTCC CAAAAGCTTA 
GAACGGGCAA CTGCTCACAA GCTCATGACA GCAATGTTGA ATGGGGATGA GCGGTTCATT 
GAGCCAACCC CACAATCATT ACAAAATTTA ACACTGAAAT CCGTAAAAGA TGCAGTGATG 
AATCAGTTTG TTGGTGGAAA CATGGAGGTA AGTATTGTTG GAGATTTCTC AGAGGAGGAG 
GTTCAGTCTT GCATTATTGA TTACCTGGGC ACAGTTAGAG CAACAAGAGA TTCTGATCAG 
GAACAAGAAT TTAATCCTGT CATGTTTCGA CCATCCCCTT CTGATTTGCA GTTTCAACAA 
GTGTTTTTGA AGGATACTGA TGAGAGAGCA TGTGCATATA TTGCTGGTCC TGCACCAAAT 
CGTTGGGGTT TCACTGTTGA TGGAACAGAC CTGTTCAAGT CAATGAGTGG CTTTTCGGTT 
TCAGCTGATG CACAACCAAT ATCTGAAACA CAACAGATAG ACGGGATGGA TGTTCAGAAG 
GATATGCAAG GAAAACTTCG CTGTCATCCA CTCTTCTTTG GCATAACAAT GGGGCTACTG 
GCTGAGATCA TAAATTCTCG GCTTTTTACC ACAGTTAGGG ATTCTCTTGG GTTGACATAT 
GATGTATCGT TTGAGTTAAG CCTATTTGAT AGGCTTAAGC TTGGTTGGTA TGTTGTATCA 
GTAACATCAA CTCCAGGCAA GGTACATAAA GCGGTTGATG CATGCAAAAG TGTTCTTAGA 
GGGTTGCATA GCAACAAGGT TGCCCAGAGA GAGTTGGACA GGGCAAGACG GACGCTGCTA 
ATGAGACATG AAGCTGAGAT TAAGTCCAAT GCCTATTGGC TTGGATTGCT TGCTCATTTG 
CAAGCCTCTT CTGTTCCGAG AAAGGACGTC TCATGTATTA AAGATCTCAC TTCACTCTAT 
GAAGCTGCCA CAATTGAGGA CATATACCTT GCATATGAAC AATTGAAAGT AGATGAGGAT 
TCTCTGTATT CATGCATTGG GGTTGCTGGG ACACAAGCTG GAGAGGAGAT TAATGCTCCA 
TTAGAAGTGG AAGAAACAGA TGATGGTTTG CAGGGAGGCA TACCTGTGGG ACGTGGTTTG 
TCTACAATGA CACGACCAAC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002301748.2
CDS	164..3970
Translation	MASTAAPSSVLMTSLPQIRTDDTFSRKNRINLIRSPLIRFQASHHQSCHCISSKRWKHEFAAGRSGTSRKKNNAWKHCSSFLGERVVEASFPEQFKCMSCSLNRRRSRYSIKRSIPRAFIDKSAFRLSGHSFDTASAKHVHVPCASMGPNEPHAASIACPDGILERQDSHLLDSELERARLLEFLNSELPCHPKLHRGQLKNGLCYLILPNKVPPNRFEAHMEVHVGSIDEEDDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTCTKDADGDLLPSVLDALNEIAFHPSFLASRVEKERRAILSELRMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKWDAEKIRKFHERWYFPANATLYIVGDIDNISKTVHQIENVFGQTGLENKTVSAPSPSAFGAMASFLAPKVSVGLPGSSSREKSSSSLDQSKIIKRERHAVRPPVEHYWSLPGSNANLKPPQIFQHEFLQNFSINMFCKIPVSKVQTNGDLCSVLMKRIFLSALHFRINTRYKSSNPPFTSVELDHSDSGREGCTVTTLTVTAEPKNWQNAIKVAVQEVRRLKEFGVTKGELTRYMDALLKDSEHLAAMIDNVSSVDNLEFIMESDALGHTVMDQRQGHESLFAVAGMVTLEEVNSIGAKLLEFISDFGKPTAPIPAAIVACVPTKVHIDGLGETEFKISSSEITAAIKSGLEEAIEAEPELEVPKELISSTQLEELRLERRPSFVPLLPDAGYTKLHDQETGITQCRLSNGIAVNYKISKSESRGGVMRLIVGGGRAAESSESKGAVVVGVRTLSEGGRVGSFSREQVELFCVNHLINCSLESTEEFICMEFRFTLRDNGMQAAFELLHMVLENSVWLDDAFDRARQLYLSYYRSIPKSLERATAHKLMTAMLNGDERFIEPTPQSLQNLTLKSVKDAVMNQFVGGNMEVSIVGDFSEEEVQSCIIDYLGTVRATRDSDQEQEFNPVMFRPSPSDLQFQQVFLKDTDERACAYIAGPAPNRWGFTVDGTDLFKSMSGFSVSADAQPISETQQIDGMDVQKDMQGKLRCHPLFFGITMGLLAEIINSRLFTTVRDSLGLTYDVSFELSLFDRLKLGWYVVSVTSTPGKVHKAVDACKSVLRGLHSNKVAQRELDRARRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDVSCIKDLTSLYEAATIEDIYLAYEQLKVDEDSLYSCIGVAGTQAGEEINAPLEVEETDDGLQGGIPVGRGLSTMTRPTT

Target ORF information:

RefSeq Version	XM_002301712.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0002s23680g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002301712.2

ORF Insert Sequence:

ATGGCGAGTA CAGCTGCCCC AAGCTCTGTA TTGATGACGA GCTTGCCTCA AATCCGTACA 
GATGATACTT TCTCAAGGAA GAACCGGATA AACCTAATTC GTTCCCCTTT GATTCGATTC 
CAAGCCAGCC ATCATCAATC TTGTCATTGC ATTTCCTCCA AAAGGTGGAA ACATGAGTTT 
GCTGCCGGTA GATCAGGTAC TAGTAGAAAG AAGAATAATG CTTGGAAACA TTGTAGTTCG 
TTCCTTGGTG AGAGAGTGGT TGAGGCATCT TTTCCAGAAC AATTTAAGTG CATGTCTTGC 
TCCCTTAACC GCCGTAGAAG CCGCTATAGC ATCAAGAGAT CTATACCTAG AGCCTTTATT 
GATAAGTCTG CTTTTCGTTT GTCTGGCCAT TCATTCGATA CTGCTTCTGC AAAGCATGTT 
CATGTCCCAT GTGCATCAAT GGGTCCAAAT GAGCCACATG CAGCAAGTAT AGCCTGCCCA 
GATGGTATTC TTGAGAGACA AGATTCCCAT TTATTAGATT CTGAATTAGA AAGAGCAAGA 
CTACTTGAGT TTTTGAATTC TGAACTTCCA TGTCACCCCA AGTTACACAG AGGACAACTG 
AAAAATGGAC TCTGTTATCT GATTCTACCA AATAAAGTTC CACCAAACAG GTTTGAGGCA 
CACATGGAAG TTCATGTAGG ATCAATAGAC GAGGAAGATG ATGAGCAAGG AATTGCACAT 
ATGATTGAAC ATGTTGCATT CCTTGGAAGT AAGAAACGTG AGAAACTTCT TGGAACAGGG 
GCCCGATCTA ATGCTTACAC TGATTTCCAC CATACAGTGT TCCACATTCA TTCACCAACT 
TGTACAAAGG ATGCTGATGG AGATCTACTC CCCTCTGTGT TGGATGCCTT GAATGAGATA 
GCTTTCCACC CGAGCTTCCT CGCTTCTCGA GTTGAAAAGG AAAGGCGTGC TATACTTTCA 
GAACTACGGA TGATGAATAC TATAGAATAT CGTGTTGACT GCCAGTTGTT ACAACATCTA 
CATTCAGAAA ACAAGCTGAG CAAAAGGTTC CCAATAGGAT TGGAAGAGCA GATTAAGAAG 
TGGGATGCAG AGAAAATTAG GAAATTCCAT GAGCGTTGGT ACTTCCCAGC AAATGCAACT 
CTATACATTG TTGGTGACAT TGATAACATT TCAAAGACGG TTCACCAAAT TGAAAATGTC 
TTTGGACAAA CTGGCCTGGA AAACAAGACA GTTTCTGCTC CTTCTCCAAG TGCATTTGGT 
GCAATGGCTA GTTTTCTTGC TCCTAAGGTC TCAGTTGGGC TCCCTGGAAG TTCATCTCGT 
GAAAAGTCAT CTAGTTCTCT AGACCAATCC AAAATCATTA AAAGGGAAAG GCATGCAGTC 
CGTCCTCCTG TCGAGCATTA TTGGTCTCTT CCTGGAAGCA ATGCAAATTT GAAGCCACCA 
CAGATATTTC AGCATGAGTT TCTTCAGAAT TTCTCAATTA ATATGTTCTG CAAGATTCCG 
GTGAGCAAGG TCCAAACGAA TGGTGACTTG TGCAGTGTTT TGATGAAGAG AATATTTCTA 
TCTGCACTGC ACTTCCGAAT TAATACAAGA TACAAGAGTT CAAATCCACC ATTCACTTCA 
GTTGAATTGG ATCATAGTGA TTCTGGAAGG GAAGGATGCA CTGTCACAAC TCTTACAGTG 
ACTGCAGAAC CCAAGAATTG GCAAAATGCA ATTAAAGTTG CTGTTCAGGA GGTTCGAAGG 
CTTAAAGAAT TTGGTGTCAC AAAGGGTGAA TTAACTCGCT ATATGGATGC ACTTTTAAAA 
GATAGCGAAC ATCTGGCAGC TATGATTGAT AATGTATCAT CTGTTGATAA TTTGGAATTT 
ATCATGGAGA GTGATGCTCT TGGCCATACT GTGATGGATC AGAGACAAGG ACATGAGAGT 
TTGTTTGCTG TTGCTGGAAT GGTCACACTT GAAGAAGTCA ATTCTATTGG TGCCAAGTTA 
TTAGAATTTA TCTCTGATTT TGGAAAACCT ACTGCGCCCA TTCCTGCAGC AATCGTTGCA 
TGTGTTCCTA CAAAAGTGCA TATTGATGGA TTGGGTGAAA CCGAGTTCAA GATATCCTCA 
AGTGAGATTA CAGCTGCCAT AAAATCAGGA TTGGAGGAAG CAATTGAGGC TGAGCCAGAG 
CTTGAAGTGC CAAAAGAATT GATATCTTCA ACACAGTTAG AGGAGTTAAG GTTAGAACGT 
AGGCCATCCT TTGTTCCCTT ACTTCCAGAT GCAGGCTATA CGAAATTGCA TGACCAAGAA 
ACAGGCATCA CTCAGTGTCG TCTTTCAAAT GGAATTGCTG TAAATTACAA GATATCCAAA 
AGTGAATCCC GGGGAGGTGT CATGCGGCTT ATTGTGGGTG GGGGCCGAGC AGCTGAAAGT 
TCCGAGTCTA AAGGAGCTGT TGTTGTGGGT GTTCGAACTC TCAGTGAGGG TGGTCGTGTT 
GGCAGCTTTT CGAGAGAGCA GGTAGAACTT TTTTGTGTGA ATCACCTAAT AAATTGCTCT 
TTGGAGTCAA CTGAGGAATT TATTTGTATG GAGTTCCGCT TCACTCTGCG AGACAATGGG 
ATGCAAGCAG CATTTGAATT ACTTCATATG GTACTTGAGA ATAGTGTCTG GCTGGATGAT 
GCATTTGACA GAGCAAGGCA ACTCTACTTG TCATATTACC GGTCCATTCC CAAAAGCTTA 
GAACGGGCAA CTGCTCACAA GCTCATGACA GCAATGTTGA ATGGGGATGA GCGGTTCATT 
GAGCCAACCC CACAATCATT ACAAAATTTA ACACTGAAAT CCGTAAAAGA TGCAGTGATG 
AATCAGTTTG TTGGTGGAAA CATGGAGGTA AGTATTGTTG GAGATTTCTC AGAGGAGGAG 
GTTCAGTCTT GCATTATTGA TTACCTGGGC ACAGTTAGAG CAACAAGAGA TTCTGATCAG 
GAACAAGAAT TTAATCCTGT CATGTTTCGA CCATCCCCTT CTGATTTGCA GTTTCAACAA 
GTGTTTTTGA AGGATACTGA TGAGAGAGCA TGTGCATATA TTGCTGGTCC TGCACCAAAT 
CGTTGGGGTT TCACTGTTGA TGGAACAGAC CTGTTCAAGT CAATGAGTGG CTTTTCGGTT 
TCAGCTGATG CACAACCAAT ATCTGAAACA CAACAGATAG ACGGGATGGA TGTTCAGAAG 
GATATGCAAG GAAAACTTCG CTGTCATCCA CTCTTCTTTG GCATAACAAT GGGGCTACTG 
GCTGAGATCA TAAATTCTCG GCTTTTTACC ACAGTTAGGG ATTCTCTTGG GTTGACATAT 
GATGTATCGT TTGAGTTAAG CCTATTTGAT AGGCTTAAGC TTGGTTGGTA TGTTGTATCA 
GTAACATCAA CTCCAGGCAA GGTACATAAA GCGGTTGATG CATGCAAAAG TGTTCTTAGA 
GGGTTGCATA GCAACAAGGT TGCCCAGAGA GAGTTGGACA GGGCAAGACG GACGCTGCTA 
ATGAGACATG AAGCTGAGAT TAAGTCCAAT GCCTATTGGC TTGGATTGCT TGCTCATTTG 
CAAGCCTCTT CTGTTCCGAG AAAGGACGTC TCATGTATTA AAGATCTCAC TTCACTCTAT 
GAAGCTGCCA CAATTGAGGA CATATACCTT GCATATGAAC AATTGAAAGT AGATGAGGAT 
TCTCTGTATT CATGCATTGG GGTTGCTGGG ACACAAGCTG GAGAGGAGAT TAATGCTCCA 
TTAGAAGTGG AAGAAACAGA TGATGGTTTG CAGGGAGGCA TACCTGTGGG ACGTGGTTTG 
TCTACAATGA CACGACCAAC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,D?jardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasj?rvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Lepl? JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouz? P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC7488593 cDNA ORF clone, Populus trichocarpa(black cottonwood)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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