Ppp1r14a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp1r14a
Entrez Gene ID	114004
Full Name	protein phosphatase 1, regulatory (inhibitor) subunit 14A
Synonyms	Cpi17
General protein information	Preferred Names protein phosphatase 1, regulatory (inhibitor) subunit 14A Names protein phosphatase 1 regulatory subunit 14A 17 kDa PKC-potentiated inhibitory protein of PP1 CPI-17 protein kinase C-potentiated inhibitor protein of 17 kDa
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	negative regulator of myosin light chain phosphatase (MLCP) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017588644.1	XP_017444133.1	protein phosphatase 1 regulatory subunit 14A isoform X1
NM_130403.1	NP_569087.1	protein phosphatase 1 regulatory subunit 14A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPP1R14A	XP_005616686.1
Mus musculus (house mouse)	Ppp1r14a	NP_081007.2
Homo sapiens (human)	PPP1R14A	NP_150281.1
Bos taurus (cattle)	PPP1R14A	NP_001179999.1
Xenopus tropicalis (tropical clawed frog)	ppp1r14a	NP_001007956.1
Rattus norvegicus (Norway rat)	Ppp1r14a	NP_569087.1

Gene Ontology
Bibliography

Related articles in PubMed

Prolonged bed rest impairs rapid CPI-17 phosphorylation and contraction in rat mesenteric resistance arteries to cause orthostatic hypotension.
Kitazawa T, Kitazawa K
Pflugers Archiv : European journal of physiology469(12)1651-1662(2017 Dec)

Rac1 regulates myosin II phosphorylation through regulation of myosin light chain phosphatase.
Shibata K, Sakai H, Huang Q, Kamata H, Chiba Y, Misawa M, Ikebe R, Ikebe M
Journal of cellular physiology230(6)1352-64(2015 Jun)

Emodin inhibits tonic tension through suppressing PKC?-mediated inhibition of myosin phosphatase in rat isolated thoracic aorta.
Lim KM, Kwon JH, Kim K, Noh JY, Kang S, Park JM, Lee MY, Bae ON, Chung JH
British journal of pharmacology171(18)4300-10(2014 Sep)

Nuclear localization of CPI-17, a protein phosphatase-1 inhibitor protein, affects histone H3 phosphorylation and corresponds to proliferation of cancer and smooth muscle cells.
Eto M, Kirkbride JA, Chugh R, Karikari NK, Kim JI
Biochemical and biophysical research communications434(1)137-42(2013 Apr)

Reciprocal regulation controlling the expression of CPI-17, a specific inhibitor protein for the myosin light chain phosphatase in vascular smooth muscle cells.
Kim JI, Urban M, Young GD, Eto M
American journal of physiology. Cell physiology303(1)C58-68(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rac1 coordinates with RhoA to increase myosin light chain (MLC) phosphorylation by inactivation of CPI-17/MYPT1 phosphatase, which decreases MLC phosphatase activity thus promoting MLC phosphorylation and cell contraction
Title: Rac1 regulates myosin II phosphorylation through regulation of myosin light chain phosphatase.

Emodin attenuates tonic tension through the blockade of PKCdelta and CPI-17-mediated MLC-phosphatase inhibition.
Title: Emodin inhibits tonic tension through suppressing PKC?-mediated inhibition of myosin phosphatase in rat isolated thoracic aorta.

Nuclear accumulation of CPI-17 is detected in proliferating vascular smooth muscle cell cultures and in neointima cells of a vascular injury model.
Title: Nuclear localization of CPI-17, a protein phosphatase-1 inhibitor protein, affects histone H3 phosphorylation and corresponds to proliferation of cancer and smooth muscle cells.

results suggest that myocardin-independent Sp1/Sp3 pathways regulate the CPI-17 transcription through multiple kinases, such as ERK1/2, p38, JNK, PKC, and ROCK in response to environmental changes
Title: Reciprocal regulation controlling the expression of CPI-17, a specific inhibitor protein for the myosin light chain phosphatase in vascular smooth muscle cells.

a novel signaling cascade that links RhoA-mediated calcium sensitivity to MEF2-dependent myocardin expression in VSMCs through a mechanism involving p38 MAPK, PP1alpha, and CPI-17.
Title: A novel RhoA/ROCK-CPI-17-MEF2C signaling pathway regulates vascular smooth muscle cell gene expression.

The following Ppp1r14a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp1r14a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00343	NM_130403.1 Latest version!	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 14A (Ppp1r14a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa00343	XM_017588644.1 Latest version!	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 14A (Ppp1r14a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp1r14a ( NM_130403.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_569087.1 Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 14A (Ppp1r14a), mRNA.

Price & Availability↑

CloneID	ORa00343
Clone ID Related Accession (Same CDS sequence)	XM_017588644.1 , NM_130403.1
Accession Version	NM_130403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1 regulatory subunit 14A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY050673.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY050673.1, AF352572.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGCGC AGCGGCTGGG CAAGCGCGTG CTGAGCAAGC TGCAGTCCCC TTCGCGTGCC 
CGCGGCCCGG GGGGCAGCCC TAGTGGGCTG CAGAAGCGGC ACGCGCGAGT CACCGTCAAA 
TACGACCGGC GAGAGCTGCA GCGGCGACTG GACGTGGAGA AGTGGATCGA CGGACGCTTG 
GAAGAGCTGT ACCGCGGCAG GGAGGCAGAC ATGCCAGATG AGGTCAACAT CGACGAGCTG 
CTGGAATTGG ACAGTGAGGA GGAAAGATGC CGGAAAATCC GGGGACTCTT GGAGGCTTGC 
CTGAATCCCA CAGAGGACTT CGTCCAGGAG CTATTGGCCA AGCTTCGGGG CCTGCACAAG 
CAGCCGGGCT TCCCGCAGCC CAGCCCCTCA GATGACCCCA GCCTGAGCCC CCGCCAGGAC 
CCGGCTCACA CTGCTCCACC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569087.1
CDS	25..468
Translation	MAAQRLGKRVLSKLQSPSRARGPGGSPSGLQKRHARVTVKYDRRELQRRLDVEKWIDGRLEELYRGREADMPDEVNIDELLELDSEEERCRKIRGLLEACLNPTEDFVQELLAKLRGLHKQPGFPQPSPSDDPSLSPRQDPAHTAPP

Target ORF information:

RefSeq Version	NM_130403.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 14A (Ppp1r14a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130403.1

ORF Insert Sequence:

ATGGCAGCGC AGCGGCTGGG CAAGCGCGTG CTGAGCAAGC TGCAGTCCCC TTCGCGTGCC 
CGCGGCCCGG GGGGCAGCCC TAGTGGGCTG CAGAAGCGGC ACGCGCGAGT CACCGTCAAA 
TACGACCGGC GAGAGCTGCA GCGGCGACTG GACGTGGAGA AGTGGATCGA CGGACGCTTG 
GAAGAGCTGT ACCGCGGCAG GGAGGCAGAC ATGCCAGATG AGGTCAACAT CGACGAGCTG 
CTGGAATTGG ACAGTGAGGA GGAAAGATGC CGGAAAATCC GGGGACTCTT GGAGGCTTGC 
CTGAATCCCA CAGAGGACTT CGTCCAGGAG CTATTGGCCA AGCTTCGGGG CCTGCACAAG 
CAGCCGGGCT TCCCGCAGCC CAGCCCCTCA GATGACCCCA GCCTGAGCCC CCGCCAGGAC 
CCGGCTCACA CTGCTCCACC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r14a ( XM_017588644.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444133.1 Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 14A (Ppp1r14a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa00343
Clone ID Related Accession (Same CDS sequence)	XM_017588644.1 , NM_130403.1
Accession Version	XM_017588644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1 regulatory subunit 14A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGC AGCGGCTGGG CAAGCGCGTG CTGAGCAAGC TGCAGTCCCC TTCGCGTGCC 
CGCGGCCCGG GGGGCAGCCC TAGTGGGCTG CAGAAGCGGC ACGCGCGAGT CACCGTCAAA 
TACGACCGGC GAGAGCTGCA GCGGCGACTG GACGTGGAGA AGTGGATCGA CGGACGCTTG 
GAAGAGCTGT ACCGCGGCAG GGAGGCAGAC ATGCCAGATG AGGTCAACAT CGACGAGCTG 
CTGGAATTGG ACAGTGAGGA GGAAAGATGC CGGAAAATCC GGGGACTCTT GGAGGCTTGC 
CTGAATCCCA CAGAGGACTT CGTCCAGGAG CTATTGGCCA AGCTTCGGGG CCTGCACAAG 
CAGCCGGGCT TCCCGCAGCC CAGCCCCTCA GATGACCCCA GCCTGAGCCC CCGCCAGGAC 
CCGGCTCACA CTGCTCCACC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444133.1
CDS	218..661
Translation	MAAQRLGKRVLSKLQSPSRARGPGGSPSGLQKRHARVTVKYDRRELQRRLDVEKWIDGRLEELYRGREADMPDEVNIDELLELDSEEERCRKIRGLLEACLNPTEDFVQELLAKLRGLHKQPGFPQPSPSDDPSLSPRQDPAHTAPP

Target ORF information:

RefSeq Version	XM_017588644.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 14A (Ppp1r14a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588644.1

ORF Insert Sequence:

ATGGCAGCGC AGCGGCTGGG CAAGCGCGTG CTGAGCAAGC TGCAGTCCCC TTCGCGTGCC 
CGCGGCCCGG GGGGCAGCCC TAGTGGGCTG CAGAAGCGGC ACGCGCGAGT CACCGTCAAA 
TACGACCGGC GAGAGCTGCA GCGGCGACTG GACGTGGAGA AGTGGATCGA CGGACGCTTG 
GAAGAGCTGT ACCGCGGCAG GGAGGCAGAC ATGCCAGATG AGGTCAACAT CGACGAGCTG 
CTGGAATTGG ACAGTGAGGA GGAAAGATGC CGGAAAATCC GGGGACTCTT GGAGGCTTGC 
CTGAATCCCA CAGAGGACTT CGTCCAGGAG CTATTGGCCA AGCTTCGGGG CCTGCACAAG 
CAGCCGGGCT TCCCGCAGCC CAGCCCCTCA GATGACCCCA GCCTGAGCCC CCGCCAGGAC 
CCGGCTCACA CTGCTCCACC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prolonged bed rest impairs rapid CPI-17 phosphorylation and contraction in rat mesenteric resistance arteries to cause orthostatic hypotension.
Pflugers Archiv : European journal of physiology469(12)1651-1662(2017 Dec)
Kitazawa T,Kitazawa K

Rac1 regulates myosin II phosphorylation through regulation of myosin light chain phosphatase.
Journal of cellular physiology230(6)1352-64(2015 Jun)
Shibata K,Sakai H,Huang Q,Kamata H,Chiba Y,Misawa M,Ikebe R,Ikebe M

Emodin inhibits tonic tension through suppressing PKC?-mediated inhibition of myosin phosphatase in rat isolated thoracic aorta.
British journal of pharmacology171(18)4300-10(2014 Sep)
Lim KM,Kwon JH,Kim K,Noh JY,Kang S,Park JM,Lee MY,Bae ON,Chung JH

Nuclear localization of CPI-17, a protein phosphatase-1 inhibitor protein, affects histone H3 phosphorylation and corresponds to proliferation of cancer and smooth muscle cells.
Biochemical and biophysical research communications434(1)137-42(2013 Apr)
Eto M,Kirkbride JA,Chugh R,Karikari NK,Kim JI

Reciprocal regulation controlling the expression of CPI-17, a specific inhibitor protein for the myosin light chain phosphatase in vascular smooth muscle cells.
American journal of physiology. Cell physiology303(1)C58-68(2012 Jul)
Kim JI,Urban M,Young GD,Eto M