Higd1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Higd1a
Entrez Gene ID	140937
Full Name	HIG1 hypoxia inducible domain family, member 1A
Synonyms	Hig1
General protein information	Preferred Names HIG1 hypoxia inducible domain family, member 1A Names HIG1 domain family member 1A, mitochondrial HIG1 domain family, member 1A hypoxia induced gene 1 hypoxia-inducible gene 1 protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	expressed in the nervous system; displays developmental regulation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_080902.4	NP_543178.2	HIG1 domain family member 1A, mitochondrial

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC101902290	XP_005205509.1
Bos taurus (cattle)	LOC788501	XP_003587982.1
Xenopus tropicalis (tropical clawed frog)	higd1a	NP_001011058.1
	HIGD1A	XP_001115209.2
Canis lupus familiaris (dog)	LOC100688295	XP_005633366.1
Bos taurus (cattle)	LOC101907084	XP_005212880.1
Bos taurus (cattle)	LOC101904447	XP_005202576.1
Bos taurus (cattle)	HIGD1A	XP_005228004.1
Bos taurus (cattle)	LOC101907436	XP_005218919.1
Mus musculus (house mouse)	Higd1a	NP_062788.1
Gallus gallus (chicken)	HIGD1A	NP_001026128.1
Danio rerio (zebrafish)	hig1	NP_956394.1
Homo sapiens (human)	HIGD1A	NP_001093138.1
Pan troglodytes (chimpanzee)	HIGD1A	XP_003309789.1
Canis lupus familiaris (dog)	HIGD1A	XP_005634369.1
Bos taurus (cattle)	LOC100337355	XP_005224108.1
Rattus norvegicus (Norway rat)	Higd1a	NP_543178.2
Bos taurus (cattle)	LOC101902273	XP_005210901.1
Bos taurus (cattle)	HIGD1D	NP_001070582.1

Gene Ontology
Bibliography

Related articles in PubMed

HIGD1A Regulates Oxygen Consumption, ROS Production, and AMPK Activity during Glucose Deprivation to Modulate Cell Survival and Tumor Growth.
Ameri K, Jahangiri A, Rajah AM, Tormos KV, Nagarajan R, Pekmezci M, Nguyen V, Wheeler ML, Murphy MP, Sanders TA, Jeffrey SS, Yeghiazarians Y, Rinaudo PF, Costello JF, Aghi MK, Maltepe E
Cell reports(2015 Feb)

Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.
Ameri K, Rajah AM, Nguyen V, Sanders TA, Jahangiri A, Delay M, Donne M, Choi HJ, Tormos KV, Yeghiazarians Y, Jeffrey SS, Rinaudo PF, Rowitch DH, Aghi M, Maltepe E
PloS one8(4)e62758(2013)

Temporal distribution of Hig-1 (hypoxia-induced gene 1) mRNA and protein in rat spinal cord: changes during postnatal life.
Bed? G, Lagos P, Agrati D
Journal of molecular neuroscience : MN47(3)666-73(2012 Jul)

The survival effect of mitochondrial Higd-1a is associated with suppression of cytochrome C release and prevention of caspase activation.
An HJ, Shin H, Jo SG, Kim YJ, Lee JO, Paik SG, Lee H
Biochimica et biophysica acta1813(12)2088-98(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found an interesting change in the protein expression pattern, shifting from neurons at P1 to glial cells at P90, which points towards a functional role for this protein in the spinal cord throughout development.
Title: Temporal distribution of Hig-1 (hypoxia-induced gene 1) mRNA and protein in rat spinal cord: changes during postnatal life.

Hig-1 protein and human- and murine-predicted sequences, as well as its characteristic expression in the Central Nervous System, are indicative of a specific role which could be related to apoptosis signaling during postnatal maturation.
Title: Characterization of hypoxia induced gene 1: expression during rat central nervous system maturation and evidence of antisense RNA expression.

The following Higd1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Higd1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00281	NM_080902.4 Latest version!	Rattus norvegicus HIG1 hypoxia inducible domain family, member 1A (Higd1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Higd1a ( NM_080902.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_543178.2 Rattus norvegicus HIG1 hypoxia inducible domain family, member 1A (Higd1a), mRNA.

Price & Availability↑

CloneID	ORa00281
Clone ID Related Accession (Same CDS sequence)	NM_080902.4
Accession Version	NM_080902.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	HIG1 domain family member 1A, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC059118.1. On Aug 31, 2012 this sequence version replaced NM_080902.3. ##Evidence-Data-START## Transcript exon combination :: BC059118.1, CF977525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCAACCA ACACAGACCT TTCTCTCTCT TCATACGATG AAGGTCAGGG GTCCAAGTTT 
ATTCGGAAAG CTAGGGAGAC ACCGTTTGTC CCCATTGGAA TGGCCGGCTT CGCAGCGATT 
GTTGCATATG GGCTCTACAA GCTGAAGAGC AGGGGAAACA CGAAGATGTC TATTCACTTG 
ATCCACATGC GTGTGGCAGC CCAGGGCTTT GTTGTGGGGG CCATGACTCT TGGTATGGGC 
TATTCCATGT ATCAAGAATT CTGGGCCAAG CGTAAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_543178.2
CDS	79..360
Translation	MSTNTDLSLSSYDEGQGSKFIRKARETPFVPIGMAGFAAIVAYGLYKLKSRGNTKMSIHLIHMRVAAQGFVVGAMTLGMGYSMYQEFWAKRKP

Target ORF information:

RefSeq Version	NM_080902.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HIG1 hypoxia inducible domain family, member 1A (Higd1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080902.4

ORF Insert Sequence:

ATGTCAACCA ACACAGACCT TTCTCTCTCT TCATACGATG AAGGTCAGGG GTCCAAGTTT 
ATTCGGAAAG CTAGGGAGAC ACCGTTTGTC CCCATTGGAA TGGCCGGCTT CGCAGCGATT 
GTTGCATATG GGCTCTACAA GCTGAAGAGC AGGGGAAACA CGAAGATGTC TATTCACTTG 
ATCCACATGC GTGTGGCAGC CCAGGGCTTT GTTGTGGGGG CCATGACTCT TGGTATGGGC 
TATTCCATGT ATCAAGAATT CTGGGCCAAG CGTAAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HIGD1A Regulates Oxygen Consumption, ROS Production, and AMPK Activity during Glucose Deprivation to Modulate Cell Survival and Tumor Growth.
Cell reports(2015 Feb)
Ameri K,Jahangiri A,Rajah AM,Tormos KV,Nagarajan R,Pekmezci M,Nguyen V,Wheeler ML,Murphy MP,Sanders TA,Jeffrey SS,Yeghiazarians Y,Rinaudo PF,Costello JF,Aghi MK,Maltepe E

Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.
PloS one8(4)e62758(2013)
Ameri K,Rajah AM,Nguyen V,Sanders TA,Jahangiri A,Delay M,Donne M,Choi HJ,Tormos KV,Yeghiazarians Y,Jeffrey SS,Rinaudo PF,Rowitch DH,Aghi M,Maltepe E

Temporal distribution of Hig-1 (hypoxia-induced gene 1) mRNA and protein in rat spinal cord: changes during postnatal life.
Journal of molecular neuroscience : MN47(3)666-73(2012 Jul)
Bed? G,Lagos P,Agrati D

The survival effect of mitochondrial Higd-1a is associated with suppression of cytochrome C release and prevention of caspase activation.
Biochimica et biophysica acta1813(12)2088-98(2011 Dec)
An HJ,Shin H,Jo SG,Kim YJ,Lee JO,Paik SG,Lee H