Serpina12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Serpina12
Entrez Gene ID	191570
Full Name	serpin family A member 12
Synonyms	Ol-64,Ol64
General protein information	Preferred Names serpin family A member 12 Names serpin A12 serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12 serine (or cysteine) peptidase inhibitor, clade A, member 12 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12 serpin peptidase inhibitor, clade A, member 12 vaspin visceral adipose tissue-derived serine protease inhibitor visceral adipose tissue-derived serpin visceral adipose-specific serpin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q32
Summary	human homolog is a serine protease inhibitor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594028.1	XP_017449517.1	serpin A12 isoform X1
NM_138825.2	NP_620180.2	serpin A12 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SERPINA12	XP_855210.1
Bos taurus (cattle)	SERPINA12	NP_001070985.1
Mus musculus (house mouse)	Serpina12	NP_080811.1
Pan troglodytes (chimpanzee)	SERPINA12	XP_001152987.1
	SERPINA12	XP_001099453.1
Rattus norvegicus (Norway rat)	Serpina12	NP_620180.2
Gallus gallus (chicken)	SERPINA3	XP_004941983.1
Homo sapiens (human)	SERPINA12	NP_776249.1

Gene Ontology
Bibliography

Related articles in PubMed

Vaspin prevents leptin?induced inflammation and catabolism by inhibiting the activation of nuclear factor??B in rat chondrocytes.
Bao JP, Xu LH, Ran JS, Xiong Y, Wu LD
Molecular medicine reports16(3)2925-2930(2017 Sep)

Visceral adipose tissue-derived serine protease inhibitor augments acetylcholine-induced relaxation via the inhibition of acetylcholine esterase activity in rat isolated mesenteric artery.
Kameshima S, Yamada K, Morita T, Okada M, Yamawaki H
Acta physiologica (Oxford, England)216(2)203-10(2016 Feb)

Vaspin as a Risk Factor of Insulin Resistance in Obstructive Sleep Apnea-Hypopnea Syndrome in an Animal Model.
Zhuang X, Ni Y, Jiang D, Pu Y, Liu X, Chen S, Sun F, Sun A, Pan Z
Clinical laboratory61(8)883-9(2015)

Vaspin attenuates high glucose-induced vascular smooth muscle cells proliferation and chemokinesis by inhibiting the MAPK, PI3K/Akt, and NF-?B signaling pathways.
Li H, Peng W, Zhuang J, Lu Y, Jian W, Wei Y, Li W, Xu Y
Atherosclerosis228(1)61-8(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We for the first time demonstrate that vaspin augments the acetylcholine -induced nitric oxide-mediated endothelium-dependent relaxation via the inhibition of acetylcholinesterase activity.
Title: Visceral adipose tissue-derived serine protease inhibitor augments acetylcholine-induced relaxation via the inhibition of acetylcholine esterase activity in rat isolated mesenteric artery.

In the chronic intermittent hypoxia rat model, the expression of vaspin in visceral adipose tissues and plasma were increased, which were correlated with insulin resistance.
Title: Vaspin as a Risk Factor of Insulin Resistance in Obstructive Sleep Apnea-Hypopnea Syndrome in an Animal Model.

Vaspin inhibits glucose-induced vascular smooth muscle cells proliferation and chemokinesis by preventing reactive oxygen species activation and MAPK, PI3K/Akt, and NF-kappaB signaling.
Title: Vaspin attenuates high glucose-induced vascular smooth muscle cells proliferation and chemokinesis by inhibiting the MAPK, PI3K/Akt, and NF-?B signaling pathways.

Vaspin is a novel ligand for the cell-surface GRP78/voltage-dependent anion channel complex in endothelial cells and promotes proliferation, inhibits apoptosis, and protects vascular injuries in diabetes mellitus.
Title: Visceral adipose tissue-derived serine proteinase inhibitor inhibits apoptosis of endothelial cells as a ligand for the cell-surface GRP78/voltage-dependent anion channel complex.

Vaspin is presumably an adipocytokine that can increase insulin sensitivity, promote insulin secretion by islet beta-cells and improve glucose tolerance, and it may be involved in insulin resistance and the disturbance of carbohydrate metabolism.
Title: [Correlation between vaspin concentration and insulin sensitivity in the visceral adipose tissue of young obese rats].

The following Serpina12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Serpina12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12300	NM_138825.2 Latest version!	Rattus norvegicus serpin family A member 12 (Serpina12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa12300	XM_017594028.1 Latest version!	Rattus norvegicus serpin family A member 12 (Serpina12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Serpina12 ( NM_138825.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620180.2 Rattus norvegicus serpin family A member 12 (Serpina12), mRNA.

Price & Availability↑

CloneID	ORa12300
Clone ID Related Accession (Same CDS sequence)	XM_017594028.1 , NM_138825.2
Accession Version	NM_138825.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Rattus norvegicus(Norway rat)
Product	serpin A12 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC094636.6. On Oct 17, 2007 this sequence version replaced NM_138825.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF245398.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGAACCTCG TGCTGGGCCT GGGTCTGTTT CTGGCAGGCC TCCTCACTGT GAAAGGTCTC 
CTGCAGGACA GAGATGCTCC AGATACATAT GAGTCTCCAG TCAGGGTCCA AGAGTGGAGG 
GGCAAGAAGG ATGCCCGGGA GCTTACTCGG CACAACATGG AATTTGGCTT CAAGCTACTG 
CAGAGGCTGG CCAGCAACAG CCGTCAGGGG AACATCTTCT TATCTCCACT GAGCATCTCA 
ACTGCCTTCT CCATGCTGTC TCTGGGGGCC CAAAACTCCA CACTGGAGGA AATCAGGGAG 
GGTTTCAATT TCAAGGAGAT GTCAGACAGG GACATGCACA TGGGTTTCCA CTACCTTCTC 
CAAAAGCTCA ACCGGGAGAC ACAGGACGTA AAGATGAGCA TAGGCAATGC TTTATTTATG 
GACCAGAGGC TGAGGCCCCA ACAGAGGTTC CTGAAACTGG CTAAGAACTT GTATGATGCG 
GACATGATAC TCACTAATTT CCAGGACTTA GAAAACACTC AGAAGAATAT CAACAAGTAC 
ATCAGTCGGA AAACCCACAA CAGAATCGAA AACATGGTCA AAAACATAGA CCCAGGGACG 
GTGATGCTCC TCACAAACTA TATTTACTTT CAAGGCAGGT GGCAGTATGA ATTCGACCCA 
AAACAAACCA AAGAGGAGGA CTTCTTCATA GAGGAAGGCA AGACCGTGAA GGTGCCCATG 
ATGTTCCAAA GGGGCATGTA CGACATGGCC TATGACAGTC AGCTCTCCTG CACCATCTTG 
GAAATGCCCT ACCGAAGAAA CATCACGGCC ACCTTTGTCC TTCCTGACAG TGGCAAACTG 
AGGCTCCTGG AGCAGGGCTT GCAAGCAGAC ATCTTTGCCA AATGGAAAAG CCTTCTCTCT 
AAAAGAGTGG TAGATGTGTG GGTGCCCAGG CTCCACATCT CGGCTACTTA CAACATGAAG 
AAGGTTCTGT CCCGCCTGGG CATCAGCAAA ATCTTTGAAG AGCATGGTGA CCTCACCCGC 
ATCTCGTCTC ATCGAAGCCT GAAGGTGGGA GAGGCTGTGC ACAAGGCTGA GCTGAGGATG 
AATGAGAAGG GCACAGAAGG AGCTGCAGGC TCGGGAGCCC AGACACTGCC CATGGAGACC 
CCACGAAGGA TGAAGCTAAA CGCACCCTTC CTGATGATGA TTTATGAGAA CCTCATGCCT 
TCCATGATCT TCTTGGCAAG GATTTATAAC CCTAGTGAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620180.2
CDS	213..1457
Translation	MNLVLGLGLFLAGLLTVKGLLQDRDAPDTYESPVRVQEWRGKKDARELTRHNMEFGFKLLQRLASNSRQGNIFLSPLSISTAFSMLSLGAQNSTLEEIREGFNFKEMSDRDMHMGFHYLLQKLNRETQDVKMSIGNALFMDQRLRPQQRFLKLAKNLYDADMILTNFQDLENTQKNINKYISRKTHNRIENMVKNIDPGTVMLLTNYIYFQGRWQYEFDPKQTKEEDFFIEEGKTVKVPMMFQRGMYDMAYDSQLSCTILEMPYRRNITATFVLPDSGKLRLLEQGLQADIFAKWKSLLSKRVVDVWVPRLHISATYNMKKVLSRLGISKIFEEHGDLTRISSHRSLKVGEAVHKAELRMNEKGTEGAAGSGAQTLPMETPRRMKLNAPFLMMIYENLMPSMIFLARIYNPSEK

Target ORF information:

RefSeq Version	NM_138825.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serpin family A member 12 (Serpina12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138825.2

ORF Insert Sequence:

ATGAACCTCG TGCTGGGCCT GGGTCTGTTT CTGGCAGGCC TCCTCACTGT GAAAGGTCTC 
CTGCAGGACA GAGATGCTCC AGATACATAT GAGTCTCCAG TCAGGGTCCA AGAGTGGAGG 
GGCAAGAAGG ATGCCCGGGA GCTTACTCGG CACAACATGG AATTTGGCTT CAAGCTACTG 
CAGAGGCTGG CCAGCAACAG CCGTCAGGGG AACATCTTCT TATCTCCACT GAGCATCTCA 
ACTGCCTTCT CCATGCTGTC TCTGGGGGCC CAAAACTCCA CACTGGAGGA AATCAGGGAG 
GGTTTCAATT TCAAGGAGAT GTCAGACAGG GACATGCACA TGGGTTTCCA CTACCTTCTC 
CAAAAGCTCA ACCGGGAGAC ACAGGACGTA AAGATGAGCA TAGGCAATGC TTTATTTATG 
GACCAGAGGC TGAGGCCCCA ACAGAGGTTC CTGAAACTGG CTAAGAACTT GTATGATGCG 
GACATGATAC TCACTAATTT CCAGGACTTA GAAAACACTC AGAAGAATAT CAACAAGTAC 
ATCAGTCGGA AAACCCACAA CAGAATCGAA AACATGGTCA AAAACATAGA CCCAGGGACG 
GTGATGCTCC TCACAAACTA TATTTACTTT CAAGGCAGGT GGCAGTATGA ATTCGACCCA 
AAACAAACCA AAGAGGAGGA CTTCTTCATA GAGGAAGGCA AGACCGTGAA GGTGCCCATG 
ATGTTCCAAA GGGGCATGTA CGACATGGCC TATGACAGTC AGCTCTCCTG CACCATCTTG 
GAAATGCCCT ACCGAAGAAA CATCACGGCC ACCTTTGTCC TTCCTGACAG TGGCAAACTG 
AGGCTCCTGG AGCAGGGCTT GCAAGCAGAC ATCTTTGCCA AATGGAAAAG CCTTCTCTCT 
AAAAGAGTGG TAGATGTGTG GGTGCCCAGG CTCCACATCT CGGCTACTTA CAACATGAAG 
AAGGTTCTGT CCCGCCTGGG CATCAGCAAA ATCTTTGAAG AGCATGGTGA CCTCACCCGC 
ATCTCGTCTC ATCGAAGCCT GAAGGTGGGA GAGGCTGTGC ACAAGGCTGA GCTGAGGATG 
AATGAGAAGG GCACAGAAGG AGCTGCAGGC TCGGGAGCCC AGACACTGCC CATGGAGACC 
CCACGAAGGA TGAAGCTAAA CGCACCCTTC CTGATGATGA TTTATGAGAA CCTCATGCCT 
TCCATGATCT TCTTGGCAAG GATTTATAAC CCTAGTGAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Serpina12 ( XM_017594028.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449517.1 Rattus norvegicus serpin family A member 12 (Serpina12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa12300
Clone ID Related Accession (Same CDS sequence)	XM_017594028.1 , NM_138825.2
Accession Version	XM_017594028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serpin A12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTCG TGCTGGGCCT GGGTCTGTTT CTGGCAGGCC TCCTCACTGT GAAAGGTCTC 
CTGCAGGACA GAGATGCTCC AGATACATAT GAGTCTCCAG TCAGGGTCCA AGAGTGGAGG 
GGCAAGAAGG ATGCCCGGGA GCTTACTCGG CACAACATGG AATTTGGCTT CAAGCTACTG 
CAGAGGCTGG CCAGCAACAG CCGTCAGGGG AACATCTTCT TATCTCCACT GAGCATCTCA 
ACTGCCTTCT CCATGCTGTC TCTGGGGGCC CAAAACTCCA CACTGGAGGA AATCAGGGAG 
GGTTTCAATT TCAAGGAGAT GTCAGACAGG GACATGCACA TGGGTTTCCA CTACCTTCTC 
CAAAAGCTCA ACCGGGAGAC ACAGGACGTA AAGATGAGCA TAGGCAATGC TTTATTTATG 
GACCAGAGGC TGAGGCCCCA ACAGAGGTTC CTGAAACTGG CTAAGAACTT GTATGATGCG 
GACATGATAC TCACTAATTT CCAGGACTTA GAAAACACTC AGAAGAATAT CAACAAGTAC 
ATCAGTCGGA AAACCCACAA CAGAATCGAA AACATGGTCA AAAACATAGA CCCAGGGACG 
GTGATGCTCC TCACAAACTA TATTTACTTT CAAGGCAGGT GGCAGTATGA ATTCGACCCA 
AAACAAACCA AAGAGGAGGA CTTCTTCATA GAGGAAGGCA AGACCGTGAA GGTGCCCATG 
ATGTTCCAAA GGGGCATGTA CGACATGGCC TATGACAGTC AGCTCTCCTG CACCATCTTG 
GAAATGCCCT ACCGAAGAAA CATCACGGCC ACCTTTGTCC TTCCTGACAG TGGCAAACTG 
AGGCTCCTGG AGCAGGGCTT GCAAGCAGAC ATCTTTGCCA AATGGAAAAG CCTTCTCTCT 
AAAAGAGTGG TAGATGTGTG GGTGCCCAGG CTCCACATCT CGGCTACTTA CAACATGAAG 
AAGGTTCTGT CCCGCCTGGG CATCAGCAAA ATCTTTGAAG AGCATGGTGA CCTCACCCGC 
ATCTCGTCTC ATCGAAGCCT GAAGGTGGGA GAGGCTGTGC ACAAGGCTGA GCTGAGGATG 
AATGAGAAGG GCACAGAAGG AGCTGCAGGC TCGGGAGCCC AGACACTGCC CATGGAGACC 
CCACGAAGGA TGAAGCTAAA CGCACCCTTC CTGATGATGA TTTATGAGAA CCTCATGCCT 
TCCATGATCT TCTTGGCAAG GATTTATAAC CCTAGTGAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449517.1
CDS	134..1378
Translation	MNLVLGLGLFLAGLLTVKGLLQDRDAPDTYESPVRVQEWRGKKDARELTRHNMEFGFKLLQRLASNSRQGNIFLSPLSISTAFSMLSLGAQNSTLEEIREGFNFKEMSDRDMHMGFHYLLQKLNRETQDVKMSIGNALFMDQRLRPQQRFLKLAKNLYDADMILTNFQDLENTQKNINKYISRKTHNRIENMVKNIDPGTVMLLTNYIYFQGRWQYEFDPKQTKEEDFFIEEGKTVKVPMMFQRGMYDMAYDSQLSCTILEMPYRRNITATFVLPDSGKLRLLEQGLQADIFAKWKSLLSKRVVDVWVPRLHISATYNMKKVLSRLGISKIFEEHGDLTRISSHRSLKVGEAVHKAELRMNEKGTEGAAGSGAQTLPMETPRRMKLNAPFLMMIYENLMPSMIFLARIYNPSEK

Target ORF information:

RefSeq Version	XM_017594028.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serpin family A member 12 (Serpina12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594028.1

ORF Insert Sequence:

ATGAACCTCG TGCTGGGCCT GGGTCTGTTT CTGGCAGGCC TCCTCACTGT GAAAGGTCTC 
CTGCAGGACA GAGATGCTCC AGATACATAT GAGTCTCCAG TCAGGGTCCA AGAGTGGAGG 
GGCAAGAAGG ATGCCCGGGA GCTTACTCGG CACAACATGG AATTTGGCTT CAAGCTACTG 
CAGAGGCTGG CCAGCAACAG CCGTCAGGGG AACATCTTCT TATCTCCACT GAGCATCTCA 
ACTGCCTTCT CCATGCTGTC TCTGGGGGCC CAAAACTCCA CACTGGAGGA AATCAGGGAG 
GGTTTCAATT TCAAGGAGAT GTCAGACAGG GACATGCACA TGGGTTTCCA CTACCTTCTC 
CAAAAGCTCA ACCGGGAGAC ACAGGACGTA AAGATGAGCA TAGGCAATGC TTTATTTATG 
GACCAGAGGC TGAGGCCCCA ACAGAGGTTC CTGAAACTGG CTAAGAACTT GTATGATGCG 
GACATGATAC TCACTAATTT CCAGGACTTA GAAAACACTC AGAAGAATAT CAACAAGTAC 
ATCAGTCGGA AAACCCACAA CAGAATCGAA AACATGGTCA AAAACATAGA CCCAGGGACG 
GTGATGCTCC TCACAAACTA TATTTACTTT CAAGGCAGGT GGCAGTATGA ATTCGACCCA 
AAACAAACCA AAGAGGAGGA CTTCTTCATA GAGGAAGGCA AGACCGTGAA GGTGCCCATG 
ATGTTCCAAA GGGGCATGTA CGACATGGCC TATGACAGTC AGCTCTCCTG CACCATCTTG 
GAAATGCCCT ACCGAAGAAA CATCACGGCC ACCTTTGTCC TTCCTGACAG TGGCAAACTG 
AGGCTCCTGG AGCAGGGCTT GCAAGCAGAC ATCTTTGCCA AATGGAAAAG CCTTCTCTCT 
AAAAGAGTGG TAGATGTGTG GGTGCCCAGG CTCCACATCT CGGCTACTTA CAACATGAAG 
AAGGTTCTGT CCCGCCTGGG CATCAGCAAA ATCTTTGAAG AGCATGGTGA CCTCACCCGC 
ATCTCGTCTC ATCGAAGCCT GAAGGTGGGA GAGGCTGTGC ACAAGGCTGA GCTGAGGATG 
AATGAGAAGG GCACAGAAGG AGCTGCAGGC TCGGGAGCCC AGACACTGCC CATGGAGACC 
CCACGAAGGA TGAAGCTAAA CGCACCCTTC CTGATGATGA TTTATGAGAA CCTCATGCCT 
TCCATGATCT TCTTGGCAAG GATTTATAAC CCTAGTGAGA AATAA