Cldn4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Cldn4
Entrez Gene ID	304407
Full Name	claudin 4
General protein information	Preferred Names claudin 4 Names claudin-4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q12

mRNA and Protein(s)

mRNA Protein Name

NM_001012022.1 NP_001012022.1 claudin-4
Pathways from BioSystems

mRNA	Protein	Name
NM_001012022.1	NP_001012022.1	claudin-4

Homologies

Homo sapiens (human)	CLDN4	NP_001296.1
Pan troglodytes (chimpanzee)	CLDN4	XP_001148188.1
Rattus norvegicus (Norway rat)	Cldn4	NP_001012022.1
Danio rerio (zebrafish)	cldna	NP_571837.2
Canis lupus familiaris (dog)	CLDN4	XP_005621019.1
Gallus gallus (chicken)	CLDN4	XP_003642430.1
	CLDN4	XP_001112477.1
Bos taurus (cattle)	CLDN4	NP_001014413.1
Danio rerio (zebrafish)	cldnb	NP_571838.1
Mus musculus (house mouse)	Cldn4	NP_034033.1

Gene Ontology
Bibliography

Related articles in PubMed

Aldosterone signaling regulates the over-expression of claudin-4 and -8 at the distal nephron from type 1 diabetic rats.
Molina-Jij?n E, Rodr?guez-Mu?oz R, Gonz?lez-Ram?rez R, Namorado-T?nix C, Pedraza-Chaverri J, Reyes JL
PloS one12(5)e0177362(2017)

Alteration of Tight Junction Protein Expression in Dahl Salt-Sensitive Rat Kidney.
Jo CH, Kim S, Oh IH, Park JS, Kim GH
Kidney & blood pressure research42(6)951-960(2017)

Claudin-4 is required for AMPK-modulated paracellular permeability in submandibular gland cells.
Xiang RL, Mei M, Cong X, Li J, Zhang Y, Ding C, Wu LL, Yu GY
Journal of molecular cell biology6(6)486-97(2014 Dec)

Hyperosmolarity-induced up-regulation of claudin-4 mediated by NADPH oxidase-dependent H2O2 production and Sp1/c-Jun cooperation.
Ikari A, Atomi K, Yamazaki Y, Sakai H, Hayashi H, Yamaguchi M, Sugatani J
Biochimica et biophysica acta1833(12)2617-2627(2013 Dec)

Disruption of ZO-1/claudin-4 interaction in relation to inflammatory responses in methotrexate-induced intestinal mucositis.
Hamada K, Kakigawa N, Sekine S, Shitara Y, Horie T
Cancer chemotherapy and pharmacology72(4)757-65(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the participation of aldosterone (ALD), and the effect of spironolactone (SPL), a mineralocorticoid receptor antagonist, on the expressions of claudin-2, -4, -5 and -8, and occludin in glomeruli, proximal and distal tubules isolated from diabetic rats, was evaluated.
Title: Aldosterone signaling regulates the over-expression of claudin-4 and -8 at the distal nephron from type 1 diabetic rats.

these results indicated that claudin-4 played a crucial role in AMPK-modulated paracellular permeability and ERK1/2 was required in AMPK-modulated tight junction barrier function in submandibular gland.
Title: Claudin-4 is required for AMPK-modulated paracellular permeability in submandibular gland cells.

hyperosmolarity increases nuclear Sp1/c-Jun complex and the association of the complex with the Sp1 binding site, resulting in the segment-specific expression of claudin-4 in the kidney.
Title: Hyperosmolarity-induced up-regulation of claudin-4 mediated by NADPH oxidase-dependent H2O2 production and Sp1/c-Jun cooperation.

elevated levels of inflammatory cytokines and chemokines in the small intestine of MTX-treated rats may contribute to inhibition of ZO-1/claudin-4 binding, and inhibition of ZO-1/claudin-4 binding may in turn lead to a reduction in claudin-4 expression.
Title: Disruption of ZO-1/claudin-4 interaction in relation to inflammatory responses in methotrexate-induced intestinal mucositis.

These results demonstrate that, in the context of the alveolar epithelium, Cldn-3 and Cldn-4 have different effects on paracellular permeability, despite significant homology in their extracellular loop domains.
Title: Differential effects of claudin-3 and claudin-4 on alveolar epithelial barrier function.

The following Cldn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cldn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13235	NM_001012022.1 Latest version!	Rattus norvegicus claudin 4 (Cldn4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cldn4 ( NM_001012022.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012022.1 Rattus norvegicus claudin 4 (Cldn4), mRNA.

Price & Availability↑

CloneID	ORa13235
Clone ID Related Accession (Same CDS sequence)	NM_001012022.1
Accession Version	NM_001012022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	claudin-4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083582.1. On Feb 11, 2005 this sequence version replaced XM_222090.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC083582.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGTCCA TGGGGCTGCA GGTGCTGGGA ATCTCCTTGG CCGTCCTGGG CTGGTTAGGA 
GTCATCCTGA GTTGTTCGCT CCCCATGTGG CGGGTGACCG CCTTCATCGG CAGCAACATC 
GTCACGGCGC AGACGAGCTG GGAAGGCCTG TGGATGAACT GCGTGGTGCA GAGCACCGGC 
CAGATGCAGT GCAAGATGTA CGACTCGATG CTGGCCCTGC CGCAGGACCT GCAGGCCGCC 
CGAGCCCTGA TGGTCATCAG CATCATCGTG GGTGCTCTGG GGATGCTTCT CTCAGTCGTA 
GGGGGCAAGT GCACCAACTG CATGGAGGAC GAGACCGTCA AGGCCAAGGT CATGATCACA 
GCCGGAGCCG TGTTCATCGT GGCAAGCATG CTGATTATGG TGCCTGTGTC CTGGACGGCA 
CACAACGTCA TCCGCGACTT CTACAACCCT CTGGTGGCTT CCGGGCAGAA AAGGGAGATG 
GGGGCCTCGC TTTACATCGG CTGGGCGGCT TCTGGGCTGC TGCTCCTGGG AGGAGGCCTC 
CTCTGCTGCA ATTGCCCACC TCGCCGCAAC GAAAAGCCCT ACTCCGCCAA GTACTCCGCC 
GCCCGCTCTG TTCCCGCCAG CAACTATGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012022.1
CDS	192..824
Translation	MASMGLQVLGISLAVLGWLGVILSCSLPMWRVTAFIGSNIVTAQTSWEGLWMNCVVQSTGQMQCKMYDSMLALPQDLQAARALMVISIIVGALGMLLSVVGGKCTNCMEDETVKAKVMITAGAVFIVASMLIMVPVSWTAHNVIRDFYNPLVASGQKREMGASLYIGWAASGLLLLGGGLLCCNCPPRRNEKPYSAKYSAARSVPASNYV

Target ORF information:

RefSeq Version	NM_001012022.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus claudin 4 (Cldn4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012022.1

ORF Insert Sequence:

ATGGCGTCCA TGGGGCTGCA GGTGCTGGGA ATCTCCTTGG CCGTCCTGGG CTGGTTAGGA 
GTCATCCTGA GTTGTTCGCT CCCCATGTGG CGGGTGACCG CCTTCATCGG CAGCAACATC 
GTCACGGCGC AGACGAGCTG GGAAGGCCTG TGGATGAACT GCGTGGTGCA GAGCACCGGC 
CAGATGCAGT GCAAGATGTA CGACTCGATG CTGGCCCTGC CGCAGGACCT GCAGGCCGCC 
CGAGCCCTGA TGGTCATCAG CATCATCGTG GGTGCTCTGG GGATGCTTCT CTCAGTCGTA 
GGGGGCAAGT GCACCAACTG CATGGAGGAC GAGACCGTCA AGGCCAAGGT CATGATCACA 
GCCGGAGCCG TGTTCATCGT GGCAAGCATG CTGATTATGG TGCCTGTGTC CTGGACGGCA 
CACAACGTCA TCCGCGACTT CTACAACCCT CTGGTGGCTT CCGGGCAGAA AAGGGAGATG 
GGGGCCTCGC TTTACATCGG CTGGGCGGCT TCTGGGCTGC TGCTCCTGGG AGGAGGCCTC 
CTCTGCTGCA ATTGCCCACC TCGCCGCAAC GAAAAGCCCT ACTCCGCCAA GTACTCCGCC 
GCCCGCTCTG TTCCCGCCAG CAACTATGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Aldosterone signaling regulates the over-expression of claudin-4 and -8 at the distal nephron from type 1 diabetic rats.
PloS one12(5)e0177362(2017)
Molina-Jij?n E,Rodr?guez-Mu?oz R,Gonz?lez-Ram?rez R,Namorado-T?nix C,Pedraza-Chaverri J,Reyes JL

Alteration of Tight Junction Protein Expression in Dahl Salt-Sensitive Rat Kidney.
Kidney & blood pressure research42(6)951-960(2017)
Jo CH,Kim S,Oh IH,Park JS,Kim GH

Claudin-4 is required for AMPK-modulated paracellular permeability in submandibular gland cells.
Journal of molecular cell biology6(6)486-97(2014 Dec)
Xiang RL,Mei M,Cong X,Li J,Zhang Y,Ding C,Wu LL,Yu GY

Hyperosmolarity-induced up-regulation of claudin-4 mediated by NADPH oxidase-dependent H2O2 production and Sp1/c-Jun cooperation.
Biochimica et biophysica acta1833(12)2617-2627(2013 Dec)
Ikari A,Atomi K,Yamazaki Y,Sakai H,Hayashi H,Yamaguchi M,Sugatani J

Disruption of ZO-1/claudin-4 interaction in relation to inflammatory responses in methotrexate-induced intestinal mucositis.
Cancer chemotherapy and pharmacology72(4)757-65(2013 Oct)
Hamada K,Kakigawa N,Sekine S,Shitara Y,Horie T

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Cldn4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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