Gpt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Gpt2
Entrez Gene ID	307759
Full Name	glutamic--pyruvic transaminase 2
Synonyms	ALT2,Cc2-5
General protein information	Preferred Names glutamic--pyruvic transaminase 2 Names alanine aminotransferase 2 glutamic pyruvate transaminase (alanine aminotransferase) 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19p11

mRNA and Protein(s)

mRNA	Protein	Name
XM_008772406.2	XP_008770628.1	alanine aminotransferase 2 isoform X1
NM_001012057.2	NP_001012057.1	alanine aminotransferase 2
XM_008772405.2	XP_008770627.1	alanine aminotransferase 2 isoform X1

Pathways from BioSystems

Homologies

	LOC716305	XP_001113757.2
Drosophila melanogaster (fruit fly)	CG1640	NP_572879.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000901	XP_316880.5
Neurospora crassa	NCU03973	XP_957612.1
Arabidopsis thaliana (thale cress)	AlaAT1	NP_173173.3
Oryza sativa (rice)	Os10g0390500	NP_001064504.1
Bos taurus (cattle)	GPT2	NP_001180116.1
Magnaporthe oryzae (rice blast fungus)	MGG_06503	XP_003717056.1
Mus musculus (house mouse)	Gpt2	NP_776291.1
Caenorhabditis elegans (roundworm)	CELE_C32F10.8	NP_001021021.1
Saccharomyces cerevisiae (baker's yeast)	ALT1	NP_013190.1
Schizosaccharomyces pombe (fission yeast)	SPBC582.08	NP_595176.1
Xenopus tropicalis (tropical clawed frog)	LOC100494152	XP_002931716.1
Homo sapiens (human)	GPT2	NP_597700.1
Rattus norvegicus (Norway rat)	Gpt2	NP_001012057.1
Arabidopsis thaliana (thale cress)	ALAAT2	NP_001185380.1
Pan troglodytes (chimpanzee)	GPT2	XP_003952890.1
Gallus gallus (chicken)	GPT2	XP_414111.3
Danio rerio (zebrafish)	gpt2	NP_001092227.2
Kluyveromyces lactis	KLLA0F19162g	XP_455940.1

Gene Ontology
Bibliography

Related articles in PubMed

Hepatic ALT isoenzymes are elevated in gluconeogenic conditions including diabetes and suppressed by insulin at the protein level.
Qian K, Zhong S, Xie K, Yu D, Yang R, Gong DW
Diabetes/metabolism research and reviews31(6)562-71(2015 Sep)

Isoform-specific alanine aminotransferase measurement can distinguish hepatic from extrahepatic injury in humans.
Rafter I, Gr?berg T, Kotronen A, Str?mmer L, Mattson CM, Kim RW, Ehrenborg E, Andersson HB, Yki-J?rvinen H, Schuppe-Koistinen I, Ekblom B, Cotgreave I, Glinghammar B
International journal of molecular medicine30(5)1241-9(2012 Nov)

Alanine aminotransferase regulation by androgens in non-hepatic tissues.
Coss CC, Bauler M, Narayanan R, Miller DD, Dalton JT
Pharmaceutical research29(4)1046-56(2012 Apr)

Increased hepatic expression is a major determinant of serum alanine aminotransferase elevation in mice with nonalcoholic steatohepatitis.
Liu R, Pan X, Whitington PF
Liver international : official journal of the International Association for the Study of the Liver29(3)337-43(2009 Mar)

Alanine aminotransferase isoenzymes: molecular cloning and quantitative analysis of tissue expression in rats and serum elevation in liver toxicity.
Yang RZ, Park S, Reagan WJ, Goldstein R, Zhong S, Lawton M, Rajamohan F, Qian K, Liu L, Gong DW
Hepatology (Baltimore, Md.)49(2)598-607(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that both Alt1 and Alt2 are induced in liver during gluconeogenesis and in diabetes (Goto-Kakizaki rats); in hepatocytes, both Alt1 and Alt2 are induced by glucocorticoid dexamethasone and are suppressed by insulin.
Title: Hepatic ALT isoenzymes are elevated in gluconeogenic conditions including diabetes and suppressed by insulin at the protein level.

The following Gpt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02081	NM_001012057.2 Latest version!	Rattus norvegicus glutamic--pyruvic transaminase 2 (Gpt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa02081	XM_008772406.2 Latest version!	Rattus norvegicus glutamic--pyruvic transaminase 2 (Gpt2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa02081	XM_008772405.2 Latest version!	Rattus norvegicus glutamic--pyruvic transaminase 2 (Gpt2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpt2 ( NM_001012057.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012057.1 Rattus norvegicus glutamic--pyruvic transaminase 2 (Gpt2), mRNA.

Price & Availability↑

CloneID	ORa02081
Clone ID Related Accession (Same CDS sequence)	XM_008772406.2 , NM_001012057.2 , XM_008772405.2
Accession Version	NM_001012057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Rattus norvegicus(Norway rat)
Product	alanine aminotransferase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131806.6. On Sep 19, 2016 this sequence version replaced NM_001012057.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGCGGG CAGCGGTGCT GGTGCGGCGG GGCTCCTGCC CCCGCGCCTC GGGCCCCTGG 
GGCCGTAGTC ACAGCAGCGC TGCAGCCGAA GCCTCGGCGG CGCTCAAGGT GCGACCAGAG 
CGTAGCCCTC GAGACCGCAT CCTCACCCTG GAGTCCATGA ACCCGCAGGT GAAGGCCGTG 
GAGTACGCTG TGCGGGGACC CATCGTGCTC AAGGCCGGCG AGATCGAGAT GGAGCTGCAG 
CGGGGTATCA AAAAACCATT CACTGAGGTA ATCCGAGCCA ACATTGGGGA TGCCCATGCT 
ATGGGCCAGC AGCCAATCAC CTTCCTTCGT CAGGTGATGG CTCTCTGCAC CTACCCGAAC 
CTGCTAAACA GCCCCAGCTT CCCGGAAGAT GCTAAGAAGC GAGCGCGGCG GATCCTGCAG 
GCTTGCGGTG GAAACAGCCT GGGGTCTTAC AGTGCGAGCC AGGGCGTTAA CTGTATCCGA 
GAGGACGTGG CAGCCTTTAT CACCAGGAGA GATGGTGTAC CTGCAGACCC AGACAACATT 
TACCTGACTA CTGGAGCGAG CGACGGTATT TCTACGATCC TGAAGCTCCT GGTCTCCGGC 
GGTGGCAAGT CACGGACCGG GGTGATGATC CCCATCCCAC AGTACCCCTT GTACTCCGCG 
GTCATCTCCG AGCTCGACGC CATCCAGGTG AACTACTATC TGGATGAGGA CAACTGCTGG 
GCTTTGAACG TGGACGAGCT CCGGCGGGCA CTGCGGCAAG CTAAAGACCA CTGTGACCCT 
AAAGTTCTCT GCATCATCAA CCCCGGAAAC CCCACAGGCC AGGTACAAAG CAGAAAGTGC 
ATAGAAGATG TGATTCACTT TGCCTGGGAA GAGAAGCTTT TTCTCCTGGC TGACGAGGTA 
TACCAGGACA ACGTGTACTC TCCGGACTGC AGATTCCACT CCTTTAAGAA AGTGCTTTAC 
CAGATGGGGC CTGAGTACTC GAGTAATGTG GAGCTTGCCT CCTTCCACTC CACCTCCAAG 
GGCTACATGG GCGAATGCGG CTACAGAGGA GGCTACATGG AGGTGATCAA TTTGCACCCC 
GAGATCAAAG GCCAGCTGGT TAAGCTCCTC TCGGTCCGCC TCTGTCCGCC AGTGTCTGGA 
CAGGCCGCGA TGGACATTGT TGTGAATCCA CCGGTGCCAG GAGAGGAGTC CTTTGAGCAA 
TTCACCAGGG AAAAAGAATC CGTCCTTGGT AATCTGGCCA AAAAAGCAAA GCTGACAGAA 
GATCTGTTTA ACCAAGTCCC GGGGATCCAG TGCAACCCCT TGCAAGGAGC CATGTATGCA 
TTCCCTCGGA TTCTCATCCC TGCCAAGGCC GTGGAGGCAG CTCAGTCCCA TAAAATGGCT 
CCAGATATGT TTTACTGCAT GAAACTCCTG GAAGAAACTG GCATCTGTGT CGTGCCTGGC 
AGTGGCTTTG GACAGCGAGA AGGCACCTAC CACTTCAGAA TGACAATTCT CCCTCCAGTG 
GAGAAACTGA AGACAGTACT CCACAAGGTG AAAGATTTTC ACCTGAAGTT CCTGGAGAAG 
TACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012057.1
CDS	23..1591
Translation	MQRAAVLVRRGSCPRASGPWGRSHSSAAAEASAALKVRPERSPRDRILTLESMNPQVKAVEYAVRGPIVLKAGEIEMELQRGIKKPFTEVIRANIGDAHAMGQQPITFLRQVMALCTYPNLLNSPSFPEDAKKRARRILQACGGNSLGSYSASQGVNCIREDVAAFITRRDGVPADPDNIYLTTGASDGISTILKLLVSGGGKSRTGVMIPIPQYPLYSAVISELDAIQVNYYLDEDNCWALNVDELRRALRQAKDHCDPKVLCIINPGNPTGQVQSRKCIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYQMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVPGEESFEQFTREKESVLGNLAKKAKLTEDLFNQVPGIQCNPLQGAMYAFPRILIPAKAVEAAQSHKMAPDMFYCMKLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLKTVLHKVKDFHLKFLEKYS

Target ORF information:

RefSeq Version	NM_001012057.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamic--pyruvic transaminase 2 (Gpt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012057.2

ORF Insert Sequence:

ATGCAGCGGG CAGCGGTGCT GGTGCGGCGG GGCTCCTGCC CCCGCGCCTC GGGCCCCTGG 
GGCCGTAGTC ACAGCAGCGC TGCAGCCGAA GCCTCGGCGG CGCTCAAGGT GCGACCAGAG 
CGTAGCCCTC GAGACCGCAT CCTCACCCTG GAGTCCATGA ACCCGCAGGT GAAGGCCGTG 
GAGTACGCTG TGCGGGGACC CATCGTGCTC AAGGCCGGCG AGATCGAGAT GGAGCTGCAG 
CGGGGTATCA AAAAACCATT CACTGAGGTA ATCCGAGCCA ACATTGGGGA TGCCCATGCT 
ATGGGCCAGC AGCCAATCAC CTTCCTTCGT CAGGTGATGG CTCTCTGCAC CTACCCGAAC 
CTGCTAAACA GCCCCAGCTT CCCGGAAGAT GCTAAGAAGC GAGCGCGGCG GATCCTGCAG 
GCTTGCGGTG GAAACAGCCT GGGGTCTTAC AGTGCGAGCC AGGGCGTTAA CTGTATCCGA 
GAGGACGTGG CAGCCTTTAT CACCAGGAGA GATGGTGTAC CTGCAGACCC AGACAACATT 
TACCTGACTA CTGGAGCGAG CGACGGTATT TCTACGATCC TGAAGCTCCT GGTCTCCGGC 
GGTGGCAAGT CACGGACCGG GGTGATGATC CCCATCCCAC AGTACCCCTT GTACTCCGCG 
GTCATCTCCG AGCTCGACGC CATCCAGGTG AACTACTATC TGGATGAGGA CAACTGCTGG 
GCTTTGAACG TGGACGAGCT CCGGCGGGCA CTGCGGCAAG CTAAAGACCA CTGTGACCCT 
AAAGTTCTCT GCATCATCAA CCCCGGAAAC CCCACAGGCC AGGTACAAAG CAGAAAGTGC 
ATAGAAGATG TGATTCACTT TGCCTGGGAA GAGAAGCTTT TTCTCCTGGC TGACGAGGTA 
TACCAGGACA ACGTGTACTC TCCGGACTGC AGATTCCACT CCTTTAAGAA AGTGCTTTAC 
CAGATGGGGC CTGAGTACTC GAGTAATGTG GAGCTTGCCT CCTTCCACTC CACCTCCAAG 
GGCTACATGG GCGAATGCGG CTACAGAGGA GGCTACATGG AGGTGATCAA TTTGCACCCC 
GAGATCAAAG GCCAGCTGGT TAAGCTCCTC TCGGTCCGCC TCTGTCCGCC AGTGTCTGGA 
CAGGCCGCGA TGGACATTGT TGTGAATCCA CCGGTGCCAG GAGAGGAGTC CTTTGAGCAA 
TTCACCAGGG AAAAAGAATC CGTCCTTGGT AATCTGGCCA AAAAAGCAAA GCTGACAGAA 
GATCTGTTTA ACCAAGTCCC GGGGATCCAG TGCAACCCCT TGCAAGGAGC CATGTATGCA 
TTCCCTCGGA TTCTCATCCC TGCCAAGGCC GTGGAGGCAG CTCAGTCCCA TAAAATGGCT 
CCAGATATGT TTTACTGCAT GAAACTCCTG GAAGAAACTG GCATCTGTGT CGTGCCTGGC 
AGTGGCTTTG GACAGCGAGA AGGCACCTAC CACTTCAGAA TGACAATTCT CCCTCCAGTG 
GAGAAACTGA AGACAGTACT CCACAAGGTG AAAGATTTTC ACCTGAAGTT CCTGGAGAAG 
TACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpt2 ( XM_008772406.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770628.1 Rattus norvegicus glutamic--pyruvic transaminase 2 (Gpt2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa02081
Clone ID Related Accession (Same CDS sequence)	XM_008772406.2 , NM_001012057.2 , XM_008772405.2
Accession Version	XM_008772406.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alanine aminotransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772406.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGGG CAGCGGTGCT GGTGCGGCGG GGCTCCTGCC CCCGCGCCTC GGGCCCCTGG 
GGCCGTAGTC ACAGCAGCGC TGCAGCCGAA GCCTCGGCGG CGCTCAAGGT GCGACCAGAG 
CGTAGCCCTC GAGACCGCAT CCTCACCCTG GAGTCCATGA ACCCGCAGGT GAAGGCCGTG 
GAGTACGCTG TGCGGGGACC CATCGTGCTC AAGGCCGGCG AGATCGAGAT GGAGCTGCAG 
CGGGGTATCA AAAAACCATT CACTGAGGTA ATCCGAGCCA ACATTGGGGA TGCCCATGCT 
ATGGGCCAGC AGCCAATCAC CTTCCTTCGT CAGGTGATGG CTCTCTGCAC CTACCCGAAC 
CTGCTAAACA GCCCCAGCTT CCCGGAAGAT GCTAAGAAGC GAGCGCGGCG GATCCTGCAG 
GCTTGCGGTG GAAACAGCCT GGGGTCTTAC AGTGCGAGCC AGGGCGTTAA CTGTATCCGA 
GAGGACGTGG CAGCCTTTAT CACCAGGAGA GATGGTGTAC CTGCAGACCC AGACAACATT 
TACCTGACTA CTGGAGCGAG CGACGGTATT TCTACGATCC TGAAGCTCCT GGTCTCCGGC 
GGTGGCAAGT CACGGACCGG GGTGATGATC CCCATCCCAC AGTACCCCTT GTACTCCGCG 
GTCATCTCCG AGCTCGACGC CATCCAGGTG AACTACTATC TGGATGAGGA CAACTGCTGG 
GCTTTGAACG TGGACGAGCT CCGGCGGGCA CTGCGGCAAG CTAAAGACCA CTGTGACCCT 
AAAGTTCTCT GCATCATCAA CCCCGGAAAC CCCACAGGCC AGGTACAAAG CAGAAAGTGC 
ATAGAAGATG TGATTCACTT TGCCTGGGAA GAGAAGCTTT TTCTCCTGGC TGACGAGGTA 
TACCAGGACA ACGTGTACTC TCCGGACTGC AGATTCCACT CCTTTAAGAA AGTGCTTTAC 
CAGATGGGGC CTGAGTACTC GAGTAATGTG GAGCTTGCCT CCTTCCACTC CACCTCCAAG 
GGCTACATGG GCGAATGCGG CTACAGAGGA GGCTACATGG AGGTGATCAA TTTGCACCCC 
GAGATCAAAG GCCAGCTGGT TAAGCTCCTC TCGGTCCGCC TCTGTCCGCC AGTGTCTGGA 
CAGGCCGCGA TGGACATTGT TGTGAATCCA CCGGTGCCAG GAGAGGAGTC CTTTGAGCAA 
TTCACCAGGG AAAAAGAATC CGTCCTTGGT AATCTGGCCA AAAAAGCAAA GCTGACAGAA 
GATCTGTTTA ACCAAGTCCC GGGGATCCAG TGCAACCCCT TGCAAGGAGC CATGTATGCA 
TTCCCTCGGA TTCTCATCCC TGCCAAGGCC GTGGAGGCAG CTCAGTCCCA TAAAATGGCT 
CCAGATATGT TTTACTGCAT GAAACTCCTG GAAGAAACTG GCATCTGTGT CGTGCCTGGC 
AGTGGCTTTG GACAGCGAGA AGGCACCTAC CACTTCAGAA TGACAATTCT CCCTCCAGTG 
GAGAAACTGA AGACAGTACT CCACAAGGTG AAAGATTTTC ACCTGAAGTT CCTGGAGAAG 
TACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770628.1
CDS	304..1872
Translation	MQRAAVLVRRGSCPRASGPWGRSHSSAAAEASAALKVRPERSPRDRILTLESMNPQVKAVEYAVRGPIVLKAGEIEMELQRGIKKPFTEVIRANIGDAHAMGQQPITFLRQVMALCTYPNLLNSPSFPEDAKKRARRILQACGGNSLGSYSASQGVNCIREDVAAFITRRDGVPADPDNIYLTTGASDGISTILKLLVSGGGKSRTGVMIPIPQYPLYSAVISELDAIQVNYYLDEDNCWALNVDELRRALRQAKDHCDPKVLCIINPGNPTGQVQSRKCIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYQMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVPGEESFEQFTREKESVLGNLAKKAKLTEDLFNQVPGIQCNPLQGAMYAFPRILIPAKAVEAAQSHKMAPDMFYCMKLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLKTVLHKVKDFHLKFLEKYS

Target ORF information:

RefSeq Version	XM_008772406.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamic--pyruvic transaminase 2 (Gpt2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772406.2

ORF Insert Sequence:

ATGCAGCGGG CAGCGGTGCT GGTGCGGCGG GGCTCCTGCC CCCGCGCCTC GGGCCCCTGG 
GGCCGTAGTC ACAGCAGCGC TGCAGCCGAA GCCTCGGCGG CGCTCAAGGT GCGACCAGAG 
CGTAGCCCTC GAGACCGCAT CCTCACCCTG GAGTCCATGA ACCCGCAGGT GAAGGCCGTG 
GAGTACGCTG TGCGGGGACC CATCGTGCTC AAGGCCGGCG AGATCGAGAT GGAGCTGCAG 
CGGGGTATCA AAAAACCATT CACTGAGGTA ATCCGAGCCA ACATTGGGGA TGCCCATGCT 
ATGGGCCAGC AGCCAATCAC CTTCCTTCGT CAGGTGATGG CTCTCTGCAC CTACCCGAAC 
CTGCTAAACA GCCCCAGCTT CCCGGAAGAT GCTAAGAAGC GAGCGCGGCG GATCCTGCAG 
GCTTGCGGTG GAAACAGCCT GGGGTCTTAC AGTGCGAGCC AGGGCGTTAA CTGTATCCGA 
GAGGACGTGG CAGCCTTTAT CACCAGGAGA GATGGTGTAC CTGCAGACCC AGACAACATT 
TACCTGACTA CTGGAGCGAG CGACGGTATT TCTACGATCC TGAAGCTCCT GGTCTCCGGC 
GGTGGCAAGT CACGGACCGG GGTGATGATC CCCATCCCAC AGTACCCCTT GTACTCCGCG 
GTCATCTCCG AGCTCGACGC CATCCAGGTG AACTACTATC TGGATGAGGA CAACTGCTGG 
GCTTTGAACG TGGACGAGCT CCGGCGGGCA CTGCGGCAAG CTAAAGACCA CTGTGACCCT 
AAAGTTCTCT GCATCATCAA CCCCGGAAAC CCCACAGGCC AGGTACAAAG CAGAAAGTGC 
ATAGAAGATG TGATTCACTT TGCCTGGGAA GAGAAGCTTT TTCTCCTGGC TGACGAGGTA 
TACCAGGACA ACGTGTACTC TCCGGACTGC AGATTCCACT CCTTTAAGAA AGTGCTTTAC 
CAGATGGGGC CTGAGTACTC GAGTAATGTG GAGCTTGCCT CCTTCCACTC CACCTCCAAG 
GGCTACATGG GCGAATGCGG CTACAGAGGA GGCTACATGG AGGTGATCAA TTTGCACCCC 
GAGATCAAAG GCCAGCTGGT TAAGCTCCTC TCGGTCCGCC TCTGTCCGCC AGTGTCTGGA 
CAGGCCGCGA TGGACATTGT TGTGAATCCA CCGGTGCCAG GAGAGGAGTC CTTTGAGCAA 
TTCACCAGGG AAAAAGAATC CGTCCTTGGT AATCTGGCCA AAAAAGCAAA GCTGACAGAA 
GATCTGTTTA ACCAAGTCCC GGGGATCCAG TGCAACCCCT TGCAAGGAGC CATGTATGCA 
TTCCCTCGGA TTCTCATCCC TGCCAAGGCC GTGGAGGCAG CTCAGTCCCA TAAAATGGCT 
CCAGATATGT TTTACTGCAT GAAACTCCTG GAAGAAACTG GCATCTGTGT CGTGCCTGGC 
AGTGGCTTTG GACAGCGAGA AGGCACCTAC CACTTCAGAA TGACAATTCT CCCTCCAGTG 
GAGAAACTGA AGACAGTACT CCACAAGGTG AAAGATTTTC ACCTGAAGTT CCTGGAGAAG 
TACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpt2 ( XM_008772405.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770627.1 Rattus norvegicus glutamic--pyruvic transaminase 2 (Gpt2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa02081
Clone ID Related Accession (Same CDS sequence)	XM_008772406.2 , NM_001012057.2 , XM_008772405.2
Accession Version	XM_008772405.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alanine aminotransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772405.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGGG CAGCGGTGCT GGTGCGGCGG GGCTCCTGCC CCCGCGCCTC GGGCCCCTGG 
GGCCGTAGTC ACAGCAGCGC TGCAGCCGAA GCCTCGGCGG CGCTCAAGGT GCGACCAGAG 
CGTAGCCCTC GAGACCGCAT CCTCACCCTG GAGTCCATGA ACCCGCAGGT GAAGGCCGTG 
GAGTACGCTG TGCGGGGACC CATCGTGCTC AAGGCCGGCG AGATCGAGAT GGAGCTGCAG 
CGGGGTATCA AAAAACCATT CACTGAGGTA ATCCGAGCCA ACATTGGGGA TGCCCATGCT 
ATGGGCCAGC AGCCAATCAC CTTCCTTCGT CAGGTGATGG CTCTCTGCAC CTACCCGAAC 
CTGCTAAACA GCCCCAGCTT CCCGGAAGAT GCTAAGAAGC GAGCGCGGCG GATCCTGCAG 
GCTTGCGGTG GAAACAGCCT GGGGTCTTAC AGTGCGAGCC AGGGCGTTAA CTGTATCCGA 
GAGGACGTGG CAGCCTTTAT CACCAGGAGA GATGGTGTAC CTGCAGACCC AGACAACATT 
TACCTGACTA CTGGAGCGAG CGACGGTATT TCTACGATCC TGAAGCTCCT GGTCTCCGGC 
GGTGGCAAGT CACGGACCGG GGTGATGATC CCCATCCCAC AGTACCCCTT GTACTCCGCG 
GTCATCTCCG AGCTCGACGC CATCCAGGTG AACTACTATC TGGATGAGGA CAACTGCTGG 
GCTTTGAACG TGGACGAGCT CCGGCGGGCA CTGCGGCAAG CTAAAGACCA CTGTGACCCT 
AAAGTTCTCT GCATCATCAA CCCCGGAAAC CCCACAGGCC AGGTACAAAG CAGAAAGTGC 
ATAGAAGATG TGATTCACTT TGCCTGGGAA GAGAAGCTTT TTCTCCTGGC TGACGAGGTA 
TACCAGGACA ACGTGTACTC TCCGGACTGC AGATTCCACT CCTTTAAGAA AGTGCTTTAC 
CAGATGGGGC CTGAGTACTC GAGTAATGTG GAGCTTGCCT CCTTCCACTC CACCTCCAAG 
GGCTACATGG GCGAATGCGG CTACAGAGGA GGCTACATGG AGGTGATCAA TTTGCACCCC 
GAGATCAAAG GCCAGCTGGT TAAGCTCCTC TCGGTCCGCC TCTGTCCGCC AGTGTCTGGA 
CAGGCCGCGA TGGACATTGT TGTGAATCCA CCGGTGCCAG GAGAGGAGTC CTTTGAGCAA 
TTCACCAGGG AAAAAGAATC CGTCCTTGGT AATCTGGCCA AAAAAGCAAA GCTGACAGAA 
GATCTGTTTA ACCAAGTCCC GGGGATCCAG TGCAACCCCT TGCAAGGAGC CATGTATGCA 
TTCCCTCGGA TTCTCATCCC TGCCAAGGCC GTGGAGGCAG CTCAGTCCCA TAAAATGGCT 
CCAGATATGT TTTACTGCAT GAAACTCCTG GAAGAAACTG GCATCTGTGT CGTGCCTGGC 
AGTGGCTTTG GACAGCGAGA AGGCACCTAC CACTTCAGAA TGACAATTCT CCCTCCAGTG 
GAGAAACTGA AGACAGTACT CCACAAGGTG AAAGATTTTC ACCTGAAGTT CCTGGAGAAG 
TACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770627.1
CDS	149..1717
Translation	MQRAAVLVRRGSCPRASGPWGRSHSSAAAEASAALKVRPERSPRDRILTLESMNPQVKAVEYAVRGPIVLKAGEIEMELQRGIKKPFTEVIRANIGDAHAMGQQPITFLRQVMALCTYPNLLNSPSFPEDAKKRARRILQACGGNSLGSYSASQGVNCIREDVAAFITRRDGVPADPDNIYLTTGASDGISTILKLLVSGGGKSRTGVMIPIPQYPLYSAVISELDAIQVNYYLDEDNCWALNVDELRRALRQAKDHCDPKVLCIINPGNPTGQVQSRKCIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYQMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVPGEESFEQFTREKESVLGNLAKKAKLTEDLFNQVPGIQCNPLQGAMYAFPRILIPAKAVEAAQSHKMAPDMFYCMKLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLKTVLHKVKDFHLKFLEKYS

Target ORF information:

RefSeq Version	XM_008772405.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamic--pyruvic transaminase 2 (Gpt2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772405.2

ORF Insert Sequence:

ATGCAGCGGG CAGCGGTGCT GGTGCGGCGG GGCTCCTGCC CCCGCGCCTC GGGCCCCTGG 
GGCCGTAGTC ACAGCAGCGC TGCAGCCGAA GCCTCGGCGG CGCTCAAGGT GCGACCAGAG 
CGTAGCCCTC GAGACCGCAT CCTCACCCTG GAGTCCATGA ACCCGCAGGT GAAGGCCGTG 
GAGTACGCTG TGCGGGGACC CATCGTGCTC AAGGCCGGCG AGATCGAGAT GGAGCTGCAG 
CGGGGTATCA AAAAACCATT CACTGAGGTA ATCCGAGCCA ACATTGGGGA TGCCCATGCT 
ATGGGCCAGC AGCCAATCAC CTTCCTTCGT CAGGTGATGG CTCTCTGCAC CTACCCGAAC 
CTGCTAAACA GCCCCAGCTT CCCGGAAGAT GCTAAGAAGC GAGCGCGGCG GATCCTGCAG 
GCTTGCGGTG GAAACAGCCT GGGGTCTTAC AGTGCGAGCC AGGGCGTTAA CTGTATCCGA 
GAGGACGTGG CAGCCTTTAT CACCAGGAGA GATGGTGTAC CTGCAGACCC AGACAACATT 
TACCTGACTA CTGGAGCGAG CGACGGTATT TCTACGATCC TGAAGCTCCT GGTCTCCGGC 
GGTGGCAAGT CACGGACCGG GGTGATGATC CCCATCCCAC AGTACCCCTT GTACTCCGCG 
GTCATCTCCG AGCTCGACGC CATCCAGGTG AACTACTATC TGGATGAGGA CAACTGCTGG 
GCTTTGAACG TGGACGAGCT CCGGCGGGCA CTGCGGCAAG CTAAAGACCA CTGTGACCCT 
AAAGTTCTCT GCATCATCAA CCCCGGAAAC CCCACAGGCC AGGTACAAAG CAGAAAGTGC 
ATAGAAGATG TGATTCACTT TGCCTGGGAA GAGAAGCTTT TTCTCCTGGC TGACGAGGTA 
TACCAGGACA ACGTGTACTC TCCGGACTGC AGATTCCACT CCTTTAAGAA AGTGCTTTAC 
CAGATGGGGC CTGAGTACTC GAGTAATGTG GAGCTTGCCT CCTTCCACTC CACCTCCAAG 
GGCTACATGG GCGAATGCGG CTACAGAGGA GGCTACATGG AGGTGATCAA TTTGCACCCC 
GAGATCAAAG GCCAGCTGGT TAAGCTCCTC TCGGTCCGCC TCTGTCCGCC AGTGTCTGGA 
CAGGCCGCGA TGGACATTGT TGTGAATCCA CCGGTGCCAG GAGAGGAGTC CTTTGAGCAA 
TTCACCAGGG AAAAAGAATC CGTCCTTGGT AATCTGGCCA AAAAAGCAAA GCTGACAGAA 
GATCTGTTTA ACCAAGTCCC GGGGATCCAG TGCAACCCCT TGCAAGGAGC CATGTATGCA 
TTCCCTCGGA TTCTCATCCC TGCCAAGGCC GTGGAGGCAG CTCAGTCCCA TAAAATGGCT 
CCAGATATGT TTTACTGCAT GAAACTCCTG GAAGAAACTG GCATCTGTGT CGTGCCTGGC 
AGTGGCTTTG GACAGCGAGA AGGCACCTAC CACTTCAGAA TGACAATTCT CCCTCCAGTG 
GAGAAACTGA AGACAGTACT CCACAAGGTG AAAGATTTTC ACCTGAAGTT CCTGGAGAAG 
TACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hepatic ALT isoenzymes are elevated in gluconeogenic conditions including diabetes and suppressed by insulin at the protein level.
Diabetes/metabolism research and reviews31(6)562-71(2015 Sep)
Qian K,Zhong S,Xie K,Yu D,Yang R,Gong DW

Isoform-specific alanine aminotransferase measurement can distinguish hepatic from extrahepatic injury in humans.
International journal of molecular medicine30(5)1241-9(2012 Nov)
Rafter I,Gr?berg T,Kotronen A,Str?mmer L,Mattson CM,Kim RW,Ehrenborg E,Andersson HB,Yki-J?rvinen H,Schuppe-Koistinen I,Ekblom B,Cotgreave I,Glinghammar B

Alanine aminotransferase regulation by androgens in non-hepatic tissues.
Pharmaceutical research29(4)1046-56(2012 Apr)
Coss CC,Bauler M,Narayanan R,Miller DD,Dalton JT

Increased hepatic expression is a major determinant of serum alanine aminotransferase elevation in mice with nonalcoholic steatohepatitis.
Liver international : official journal of the International Association for the Study of the Liver29(3)337-43(2009 Mar)
Liu R,Pan X,Whitington PF

Alanine aminotransferase isoenzymes: molecular cloning and quantitative analysis of tissue expression in rats and serum elevation in liver toxicity.
Hepatology (Baltimore, Md.)49(2)598-607(2009 Feb)
Yang RZ,Park S,Reagan WJ,Goldstein R,Zhong S,Lawton M,Rajamohan F,Qian K,Liu L,Gong DW

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Gpt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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