Best3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Best3
Entrez Gene ID	314847
Full Name	bestrophin 3
Synonyms	Vmd2l3
General protein information	Preferred Names bestrophin 3 Names bestrophin-3 vitelliform macular dystrophy 2-like 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191783.1	NP_001178712.1	bestrophin-3
XM_017594852.1	XP_017450341.1	bestrophin-3 isoform X5
XM_017594849.1	XP_017450338.1	bestrophin-3 isoform X2
XM_017594850.1	XP_017450339.1	bestrophin-3 isoform X3
XM_017594851.1	XP_017450340.1	bestrophin-3 isoform X4
XM_017594848.1	XP_017450337.1	bestrophin-3 isoform X1

Pathways from BioSystems

Homologies

	BEST3	XP_001117392.1
Pan troglodytes (chimpanzee)	BEST3	XP_522466.2
Mus musculus (house mouse)	Best3	NP_001007584.1
Rattus norvegicus (Norway rat)	Best3	NP_001178712.1
Canis lupus familiaris (dog)	BEST3	XP_538279.3
Gallus gallus (chicken)	BEST3	NP_001186598.1
Xenopus tropicalis (tropical clawed frog)	best3	NP_001155224.1
Bos taurus (cattle)	BEST3	XP_002687445.1
Homo sapiens (human)	BEST3	NP_116124.2
Drosophila melanogaster (fruit fly)	Best3	NP_730039.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondria dependent pathway is involved in the protective effect of bestrophin-3 on hydrogen peroxide-induced apoptosis in basilar artery smooth muscle cells.
Jiang L, Liu Y, Ma MM, Tang YB, Zhou JG, Guan YY
Apoptosis : an international journal on programmed cell death18(5)556-65(2013 May)

ERK1/2-dependent bestrophin-3 expression prevents ER-stress-induced cell death in renal epithelial cells by reducing CHOP.
Lee WK, Chakraborty PK, Roussa E, Wolff NA, Th?venod F
Biochimica et biophysica acta1823(10)1864-76(2012 Oct)

Bestrophin is important for the rhythmic but not the tonic contraction in rat mesenteric small arteries.
Broegger T, Jacobsen JC, Secher Dam V, Boedtkjer DM, Kold-Petersen H, Pedersen FS, Aalkjaer C, Matchkov VV
Cardiovascular research91(4)685-93(2011 Sep)

Bestrophin-3 (vitelliform macular dystrophy 2-like 3 protein) is essential for the cGMP-dependent calcium-activated chloride conductance in vascular smooth muscle cells.
Matchkov VV, Larsen P, Bouzinova EV, Rojek A, Boedtkjer DM, Golubinskaya V, Pedersen FS, Aalkjaer C, Nilsson H
Circulation research103(8)864-72(2008 Oct)

A short motif in the C-terminus of mouse bestrophin 3 [corrected] inhibits its activation as a Cl channel.
Qu Z, Cui Y, Hartzell C
FEBS letters580(8)2141-6(2006 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Best-3 inhibits apoptosis induced by H2O2 in basilar artery smooth muscle cells through mitochondria dependent pathway.
Title: Mitochondria dependent pathway is involved in the protective effect of bestrophin-3 on hydrogen peroxide-induced apoptosis in basilar artery smooth muscle cells.

ERK1/2-dependent Best-3 activation regulates cell fate decisions during endoplasmic reticulum stress by suppressing CHOP induction and death.
Title: ERK1/2-dependent bestrophin-3 expression prevents ER-stress-induced cell death in renal epithelial cells by reducing CHOP.

Bestrophin-3 expression was significantly reduced compared with arteries transfected with mutated siRNA
Title: Bestrophin is important for the rhythmic but not the tonic contraction in rat mesenteric small arteries.

Bestrophin-3 (vitelliform macular dystrophy 2-like 3 protein) is essential for the cGMP-dependent calcium-activated chloride conductance in vascular smooth muscle cells.
Title: Bestrophin-3 (vitelliform macular dystrophy 2-like 3 protein) is essential for the cGMP-dependent calcium-activated chloride conductance in vascular smooth muscle cells.

The following Best3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Best3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa47365	XM_017594852.1 Latest version!	Rattus norvegicus bestrophin 3 (Best3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa07644	NM_001191783.1 Latest version!	Rattus norvegicus bestrophin 3 (Best3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa07644	XM_017594849.1 Latest version!	Rattus norvegicus bestrophin 3 (Best3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa47364	XM_017594848.1 Latest version!	Rattus norvegicus bestrophin 3 (Best3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa62012	XM_017594850.1 Latest version!	Rattus norvegicus bestrophin 3 (Best3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa62013	XM_017594851.1 Latest version!	Rattus norvegicus bestrophin 3 (Best3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Best3 ( XM_017594852.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450341.1 Rattus norvegicus bestrophin 3 (Best3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa47365
Clone ID Related Accession (Same CDS sequence)	XM_017594852.1
Accession Version	XM_017594852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bestrophin-3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATTT TTCTTCCCTT GGGAGACTCC ATAGAAATAG GGTTTTACGT TACTCTGGTA 
GTGAACCGAT GGTGGAACCA GTTTGTGAAT CTGCCTTGGC CAGACAGGCT GATGTTCCTC 
ATCTCCAGCA GTGTCCACGG GAGCGACCAG CATGGGCGCC TGCTCAGAAG GACGCTGATG 
CGCTACGTCA ATCTCACGTC CTTGCTCATC TTCCGCTCGG TGAGCACAGC GGTGTACAAA 
AGATTCCCCA CCATGGACCA CGTGGTCGAA GCAGGTTTTA TGACGGCAGA TGAGAGGAAG 
TTATTCGACC ACCTCAAGTC GCCTCATCTG AAATACTGGG TTCCGTTCAT TTGGTTTGGA 
AATCTTGCAA CCAAAGCCCG GAATGAAGGC AGAATCAGAG ACAGCGTCGA TCTACAATCA 
CTGATGACCG AAATGAATCG GTACCGCTCT TGGTGCAGCC TCTTATTTGG TTACGACTGG 
GTTGGCATTC CACTGGTTTA CACACAGGTC GTCACTCTCG CCGTCTACAC GTTCTTCTTT 
GCATGCCTGA TCGGACGGCA GTTCCTGGAT CCCACCAAAG GCTACGCGGG ACACGACTTG 
GACCTGTACA TTCCCATCTT CACCCTCCTC CAGTTCTTCT TCTACGCAGG ATGGCTCAAG 
GTTGCCGAGC AGCTGATCAA CCCTTTCGGG GAAGACGACG ACGACTTCGA AACAAACTGG 
TGCATTGACA GAAACCTGCA GGTCTCTCTT CTGGCTGTGG ATGAAATGCA CATGAGTTTA 
CCCAAGATGA AGAAGGACAT TTACTGGGAC GACTCTGCTG CTCGGCCACC GTACACGCTG 
GCAGCGGCTG ACTACTGTAT ACCTTCCTTC CTGGGCTCAA CAATCCAAAT GGGGCTGTCC 
GGGTCCGACT TTCCCGCGGA GGATTGGCTG TGGAACTATG AGAAGCAAGG CCACCGGCAC 
TCGGTGATGA GGAGGGTCAA GCGTTTCCTG AGTACCCACG AGCACCCCGG CAGTCCCAAG 
AGGAGGAGGA GTTTCGGCAG GCAGGCCAGT GACAGCTCCA TGTTCTTCCC CCCAAGCCCA 
GCTCGGGATC TGCTGAATGT GCCCTCAAGG AACCCCCACC GAGGCTCACC TACCCGGAAG 
CAATCCCACT CCCAGGAGGG CAGCCCTAAG CTTCACTCCA GCATGGGAGA ATTGTCCACC 
ATCAGGGAGA CCAGCCGCAC CAGCACTCTG CAGAGCCTGA GCCAGCAGTC CAGTGTGAGG 
GCCTCCCCTA CCAAAATGCC CCAGGTCCCC GAGGTCCTAA TCACTGCAGC CGAAGCCCCG 
GAGGCGGCGT TCGCATCAGA CAACCATCAG CGTGACTCCA CTACCTCCAT CTTGAGCCTC 
GAGTTCACTG GGGTGCAGGC GAGCGGGACT GAGCAGCAGG AAGACCCCTC GGGGAAGGTG 
ACACCTCCTG GGGACCCCGA ACCCCAGACT ATTTCAGCCG GGACTGAAAA AGACTTGTTC 
AAGTTTGAGG AAGATCTAGA TGATGACATA TTCCCCAAAA GGTGGAGCCT ACCAGTGTTT 
CGGGAGTCCA GGCACACCTC CCTAGGAAAC TTAGGTCCGG ATCCTGTGAG TCCAGAGAAT 
GCTCTTTTAC TTGACACAGA AACATCCTCA GAGACCAACG GAGTCCATCC TGGGGCTGGC 
TCTGCCGTCT CTCCCGACAT CCTGTATTTA ATGGAAAGTT TGGATAAGGA AACGGACATC 
CTGGAGTTTA ACAACGAGCA CACTGAAGAA CCCCTTAAGG GAACACCGCC GCGCCCCAGG 
ACCTGGTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450341.1
CDS	282..2093
Translation	MKIFLPLGDSIEIGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDQHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTADERKLFDHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTIQMGLSGSDFPAEDWLWNYEKQGHRHSVMRRVKRFLSTHEHPGSPKRRRSFGRQASDSSMFFPPSPARDLLNVPSRNPHRGSPTRKQSHSQEGSPKLHSSMGELSTIRETSRTSTLQSLSQQSSVRASPTKMPQVPEVLITAAEAPEAAFASDNHQRDSTTSILSLEFTGVQASGTEQQEDPSGKVTPPGDPEPQTISAGTEKDLFKFEEDLDDDIFPKRWSLPVFRESRHTSLGNLGPDPVSPENALLLDTETSSETNGVHPGAGSAVSPDILYLMESLDKETDILEFNNEHTEEPLKGTPPRPRTWF

Target ORF information:

RefSeq Version	XM_017594852.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bestrophin 3 (Best3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594852.1

ORF Insert Sequence:

ATGAAGATTT TTCTTCCCTT GGGAGACTCC ATAGAAATAG GGTTTTACGT TACTCTGGTA 
GTGAACCGAT GGTGGAACCA GTTTGTGAAT CTGCCTTGGC CAGACAGGCT GATGTTCCTC 
ATCTCCAGCA GTGTCCACGG GAGCGACCAG CATGGGCGCC TGCTCAGAAG GACGCTGATG 
CGCTACGTCA ATCTCACGTC CTTGCTCATC TTCCGCTCGG TGAGCACAGC GGTGTACAAA 
AGATTCCCCA CCATGGACCA CGTGGTCGAA GCAGGTTTTA TGACGGCAGA TGAGAGGAAG 
TTATTCGACC ACCTCAAGTC GCCTCATCTG AAATACTGGG TTCCGTTCAT TTGGTTTGGA 
AATCTTGCAA CCAAAGCCCG GAATGAAGGC AGAATCAGAG ACAGCGTCGA TCTACAATCA 
CTGATGACCG AAATGAATCG GTACCGCTCT TGGTGCAGCC TCTTATTTGG TTACGACTGG 
GTTGGCATTC CACTGGTTTA CACACAGGTC GTCACTCTCG CCGTCTACAC GTTCTTCTTT 
GCATGCCTGA TCGGACGGCA GTTCCTGGAT CCCACCAAAG GCTACGCGGG ACACGACTTG 
GACCTGTACA TTCCCATCTT CACCCTCCTC CAGTTCTTCT TCTACGCAGG ATGGCTCAAG 
GTTGCCGAGC AGCTGATCAA CCCTTTCGGG GAAGACGACG ACGACTTCGA AACAAACTGG 
TGCATTGACA GAAACCTGCA GGTCTCTCTT CTGGCTGTGG ATGAAATGCA CATGAGTTTA 
CCCAAGATGA AGAAGGACAT TTACTGGGAC GACTCTGCTG CTCGGCCACC GTACACGCTG 
GCAGCGGCTG ACTACTGTAT ACCTTCCTTC CTGGGCTCAA CAATCCAAAT GGGGCTGTCC 
GGGTCCGACT TTCCCGCGGA GGATTGGCTG TGGAACTATG AGAAGCAAGG CCACCGGCAC 
TCGGTGATGA GGAGGGTCAA GCGTTTCCTG AGTACCCACG AGCACCCCGG CAGTCCCAAG 
AGGAGGAGGA GTTTCGGCAG GCAGGCCAGT GACAGCTCCA TGTTCTTCCC CCCAAGCCCA 
GCTCGGGATC TGCTGAATGT GCCCTCAAGG AACCCCCACC GAGGCTCACC TACCCGGAAG 
CAATCCCACT CCCAGGAGGG CAGCCCTAAG CTTCACTCCA GCATGGGAGA ATTGTCCACC 
ATCAGGGAGA CCAGCCGCAC CAGCACTCTG CAGAGCCTGA GCCAGCAGTC CAGTGTGAGG 
GCCTCCCCTA CCAAAATGCC CCAGGTCCCC GAGGTCCTAA TCACTGCAGC CGAAGCCCCG 
GAGGCGGCGT TCGCATCAGA CAACCATCAG CGTGACTCCA CTACCTCCAT CTTGAGCCTC 
GAGTTCACTG GGGTGCAGGC GAGCGGGACT GAGCAGCAGG AAGACCCCTC GGGGAAGGTG 
ACACCTCCTG GGGACCCCGA ACCCCAGACT ATTTCAGCCG GGACTGAAAA AGACTTGTTC 
AAGTTTGAGG AAGATCTAGA TGATGACATA TTCCCCAAAA GGTGGAGCCT ACCAGTGTTT 
CGGGAGTCCA GGCACACCTC CCTAGGAAAC TTAGGTCCGG ATCCTGTGAG TCCAGAGAAT 
GCTCTTTTAC TTGACACAGA AACATCCTCA GAGACCAACG GAGTCCATCC TGGGGCTGGC 
TCTGCCGTCT CTCCCGACAT CCTGTATTTA ATGGAAAGTT TGGATAAGGA AACGGACATC 
CTGGAGTTTA ACAACGAGCA CACTGAAGAA CCCCTTAAGG GAACACCGCC GCGCCCCAGG 
ACCTGGTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Best3 ( NM_001191783.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178712.1 Rattus norvegicus bestrophin 3 (Best3), mRNA.

Price & Availability↑

CloneID	ORa07644
Clone ID Related Accession (Same CDS sequence)	NM_001191783.1 , XM_017594849.1
Accession Version	NM_001191783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	bestrophin-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07057196.1. On Jul 17, 2010 this sequence version replaced XM_235161.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN04563758, SAMN04563759 [ECO:0000348] ##Evidence-Data-END##

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCGACTT TTTTTGGATT TCATAGGTTG 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTGCTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTACA CAGCAATCAG TCTGGTGTAC AGATTGTTAC TTACAGGAGC CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTACGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATCTG 
CCTTGGCCAG ACAGGCTGAT GTTCCTCATC TCCAGCAGTG TCCACGGGAG CGACCAGCAT 
GGGCGCCTGC TCAGAAGGAC GCTGATGCGC TACGTCAATC TCACGTCCTT GCTCATCTTC 
CGCTCGGTGA GCACAGCGGT GTACAAAAGA TTCCCCACCA TGGACCACGT GGTCGAAGCA 
GGTTTTATGA CGGCAGATGA GAGGAAGTTA TTCGACCACC TCAAGTCGCC TCATCTGAAA 
TACTGGGTTC CGTTCATTTG GTTTGGAAAT CTTGCAACCA AAGCCCGGAA TGAAGGCAGA 
ATCAGAGACA GCGTCGATCT ACAATCACTG ATGACCGAAA TGAATCGGTA CCGCTCTTGG 
TGCAGCCTCT TATTTGGTTA CGACTGGGTT GGCATTCCAC TGGTTTACAC ACAGGTCGTC 
ACTCTCGCCG TCTACACGTT CTTCTTTGCA TGCCTGATCG GACGGCAGTT CCTGGATCCC 
ACCAAAGGCT ACGCGGGACA CGACTTGGAC CTGTACATTC CCATCTTCAC CCTCCTCCAG 
TTCTTCTTCT ACGCAGGATG GCTCAAGGTT GCCGAGCAGC TGATCAACCC TTTCGGGGAA 
GACGACGACG ACTTCGAAAC AAACTGGTGC ATTGACAGAA ACCTGCAGGT CTCTCTTCTG 
GCTGTGGATG AAATGCACAT GAGTTTACCC AAGATGAAGA AGGACATTTA CTGGGACGAC 
TCTGCTGCTC GGCCACCGTA CACGCTGGCA GCGGCTGACT ACTGTATACC TTCCTTCCTG 
GGCTCAACAA TCCAAATGGG GCTGTCCGGG TCCGACTTTC CCGCGGAGGA TTGGCTGTGG 
AACTATGAGA AGCAAGGCCA CCGGCACTCG GTGATGAGGA GGGTCAAGCG TTTCCTGAGT 
ACCCACGAGC ACCCCGGCAG TCCCAAGAGG AGGAGGAGTT TCGGCAGGCA GGCCAGTGAC 
AGCTCCATGT TCTTCCCCCC AAGCCCAGCT CGGGATCTGC TGAATGTGCC CTCAAGGAAC 
CCCCACCGAG GCTCACCTAC CCGGAAGCAA TCCCACTCCC AGGAGGGCAG CCCTAAGCTT 
CACTCCAGCA TGGGAGAATT GTCCACCATC AGGGAGACCA GCCGCACCAG CACTCTGCAG 
AGCCTGAGCC AGCAGTCCAG TGTGAGGGCC TCCCCTACCA AAATGCCCCA GGTCCCCGAG 
GTCCTAATCA CTGCAGCCGA AGCCCCGGAG GCGGCGTTCG CATCAGACAA CCATCAGCGT 
GACTCCACTA CCTCCATCTT GAGCCTCGAG TTCACTGGGG TGCAGGCGAG CGGGACTGAG 
CAGCAGGAAG ACCCCTCGGG GAAGGTGACA CCTCCTGGGG ACCCCGAACC CCAGACTATT 
TCAGCCGGGA CTGAAAAAGA CTTGTTCAAG TTTGAGGAAG ATCTAGATGA TGACATATTC 
CCCAAAAGGT GGAGCCTACC AGTGTTTCGG GAGTCCAGGC ACACCTCCCT AGGAAACTTA 
GGTCCGGATC CTGTGAGTCC AGAGAATGCT CTTTTACTTG ACACAGAAAC ATCCTCAGAG 
ACCAACGGAG TCCATCCTGG GGCTGGCTCT GCCGTCTCTC CCGACATCCT GTATTTAATG 
GAAAGTTTGG ATAAGGAAAC GGACATCCTG GAGTTTAACA ACGAGCACAC TGAAGAACCC 
CTTAAGGGAA CACCGCCGCG CCCCAGGACC TGGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178712.1
CDS	1..2019
Translation	MTVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTGAQKRYFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDQHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTADERKLFDHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTIQMGLSGSDFPAEDWLWNYEKQGHRHSVMRRVKRFLSTHEHPGSPKRRRSFGRQASDSSMFFPPSPARDLLNVPSRNPHRGSPTRKQSHSQEGSPKLHSSMGELSTIRETSRTSTLQSLSQQSSVRASPTKMPQVPEVLITAAEAPEAAFASDNHQRDSTTSILSLEFTGVQASGTEQQEDPSGKVTPPGDPEPQTISAGTEKDLFKFEEDLDDDIFPKRWSLPVFRESRHTSLGNLGPDPVSPENALLLDTETSSETNGVHPGAGSAVSPDILYLMESLDKETDILEFNNEHTEEPLKGTPPRPRTWF

Target ORF information:

RefSeq Version	NM_001191783.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bestrophin 3 (Best3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191783.1

ORF Insert Sequence:

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCGACTT TTTTTGGATT TCATAGGTTG 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTGCTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTACA CAGCAATCAG TCTGGTGTAC AGATTGTTAC TTACAGGAGC CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTACGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATCTG 
CCTTGGCCAG ACAGGCTGAT GTTCCTCATC TCCAGCAGTG TCCACGGGAG CGACCAGCAT 
GGGCGCCTGC TCAGAAGGAC GCTGATGCGC TACGTCAATC TCACGTCCTT GCTCATCTTC 
CGCTCGGTGA GCACAGCGGT GTACAAAAGA TTCCCCACCA TGGACCACGT GGTCGAAGCA 
GGTTTTATGA CGGCAGATGA GAGGAAGTTA TTCGACCACC TCAAGTCGCC TCATCTGAAA 
TACTGGGTTC CGTTCATTTG GTTTGGAAAT CTTGCAACCA AAGCCCGGAA TGAAGGCAGA 
ATCAGAGACA GCGTCGATCT ACAATCACTG ATGACCGAAA TGAATCGGTA CCGCTCTTGG 
TGCAGCCTCT TATTTGGTTA CGACTGGGTT GGCATTCCAC TGGTTTACAC ACAGGTCGTC 
ACTCTCGCCG TCTACACGTT CTTCTTTGCA TGCCTGATCG GACGGCAGTT CCTGGATCCC 
ACCAAAGGCT ACGCGGGACA CGACTTGGAC CTGTACATTC CCATCTTCAC CCTCCTCCAG 
TTCTTCTTCT ACGCAGGATG GCTCAAGGTT GCCGAGCAGC TGATCAACCC TTTCGGGGAA 
GACGACGACG ACTTCGAAAC AAACTGGTGC ATTGACAGAA ACCTGCAGGT CTCTCTTCTG 
GCTGTGGATG AAATGCACAT GAGTTTACCC AAGATGAAGA AGGACATTTA CTGGGACGAC 
TCTGCTGCTC GGCCACCGTA CACGCTGGCA GCGGCTGACT ACTGTATACC TTCCTTCCTG 
GGCTCAACAA TCCAAATGGG GCTGTCCGGG TCCGACTTTC CCGCGGAGGA TTGGCTGTGG 
AACTATGAGA AGCAAGGCCA CCGGCACTCG GTGATGAGGA GGGTCAAGCG TTTCCTGAGT 
ACCCACGAGC ACCCCGGCAG TCCCAAGAGG AGGAGGAGTT TCGGCAGGCA GGCCAGTGAC 
AGCTCCATGT TCTTCCCCCC AAGCCCAGCT CGGGATCTGC TGAATGTGCC CTCAAGGAAC 
CCCCACCGAG GCTCACCTAC CCGGAAGCAA TCCCACTCCC AGGAGGGCAG CCCTAAGCTT 
CACTCCAGCA TGGGAGAATT GTCCACCATC AGGGAGACCA GCCGCACCAG CACTCTGCAG 
AGCCTGAGCC AGCAGTCCAG TGTGAGGGCC TCCCCTACCA AAATGCCCCA GGTCCCCGAG 
GTCCTAATCA CTGCAGCCGA AGCCCCGGAG GCGGCGTTCG CATCAGACAA CCATCAGCGT 
GACTCCACTA CCTCCATCTT GAGCCTCGAG TTCACTGGGG TGCAGGCGAG CGGGACTGAG 
CAGCAGGAAG ACCCCTCGGG GAAGGTGACA CCTCCTGGGG ACCCCGAACC CCAGACTATT 
TCAGCCGGGA CTGAAAAAGA CTTGTTCAAG TTTGAGGAAG ATCTAGATGA TGACATATTC 
CCCAAAAGGT GGAGCCTACC AGTGTTTCGG GAGTCCAGGC ACACCTCCCT AGGAAACTTA 
GGTCCGGATC CTGTGAGTCC AGAGAATGCT CTTTTACTTG ACACAGAAAC ATCCTCAGAG 
ACCAACGGAG TCCATCCTGG GGCTGGCTCT GCCGTCTCTC CCGACATCCT GTATTTAATG 
GAAAGTTTGG ATAAGGAAAC GGACATCCTG GAGTTTAACA ACGAGCACAC TGAAGAACCC 
CTTAAGGGAA CACCGCCGCG CCCCAGGACC TGGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Best3 ( XM_017594849.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450338.1 Rattus norvegicus bestrophin 3 (Best3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa07644
Clone ID Related Accession (Same CDS sequence)	NM_001191783.1 , XM_017594849.1
Accession Version	XM_017594849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bestrophin-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCGACTT TTTTTGGATT TCATAGGTTG 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTGCTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTACA CAGCAATCAG TCTGGTGTAC AGATTGTTAC TTACAGGAGC CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTACGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATCTG 
CCTTGGCCAG ACAGGCTGAT GTTCCTCATC TCCAGCAGTG TCCACGGGAG CGACCAGCAT 
GGGCGCCTGC TCAGAAGGAC GCTGATGCGC TACGTCAATC TCACGTCCTT GCTCATCTTC 
CGCTCGGTGA GCACAGCGGT GTACAAAAGA TTCCCCACCA TGGACCACGT GGTCGAAGCA 
GGTTTTATGA CGGCAGATGA GAGGAAGTTA TTCGACCACC TCAAGTCGCC TCATCTGAAA 
TACTGGGTTC CGTTCATTTG GTTTGGAAAT CTTGCAACCA AAGCCCGGAA TGAAGGCAGA 
ATCAGAGACA GCGTCGATCT ACAATCACTG ATGACCGAAA TGAATCGGTA CCGCTCTTGG 
TGCAGCCTCT TATTTGGTTA CGACTGGGTT GGCATTCCAC TGGTTTACAC ACAGGTCGTC 
ACTCTCGCCG TCTACACGTT CTTCTTTGCA TGCCTGATCG GACGGCAGTT CCTGGATCCC 
ACCAAAGGCT ACGCGGGACA CGACTTGGAC CTGTACATTC CCATCTTCAC CCTCCTCCAG 
TTCTTCTTCT ACGCAGGATG GCTCAAGGTT GCCGAGCAGC TGATCAACCC TTTCGGGGAA 
GACGACGACG ACTTCGAAAC AAACTGGTGC ATTGACAGAA ACCTGCAGGT CTCTCTTCTG 
GCTGTGGATG AAATGCACAT GAGTTTACCC AAGATGAAGA AGGACATTTA CTGGGACGAC 
TCTGCTGCTC GGCCACCGTA CACGCTGGCA GCGGCTGACT ACTGTATACC TTCCTTCCTG 
GGCTCAACAA TCCAAATGGG GCTGTCCGGG TCCGACTTTC CCGCGGAGGA TTGGCTGTGG 
AACTATGAGA AGCAAGGCCA CCGGCACTCG GTGATGAGGA GGGTCAAGCG TTTCCTGAGT 
ACCCACGAGC ACCCCGGCAG TCCCAAGAGG AGGAGGAGTT TCGGCAGGCA GGCCAGTGAC 
AGCTCCATGT TCTTCCCCCC AAGCCCAGCT CGGGATCTGC TGAATGTGCC CTCAAGGAAC 
CCCCACCGAG GCTCACCTAC CCGGAAGCAA TCCCACTCCC AGGAGGGCAG CCCTAAGCTT 
CACTCCAGCA TGGGAGAATT GTCCACCATC AGGGAGACCA GCCGCACCAG CACTCTGCAG 
AGCCTGAGCC AGCAGTCCAG TGTGAGGGCC TCCCCTACCA AAATGCCCCA GGTCCCCGAG 
GTCCTAATCA CTGCAGCCGA AGCCCCGGAG GCGGCGTTCG CATCAGACAA CCATCAGCGT 
GACTCCACTA CCTCCATCTT GAGCCTCGAG TTCACTGGGG TGCAGGCGAG CGGGACTGAG 
CAGCAGGAAG ACCCCTCGGG GAAGGTGACA CCTCCTGGGG ACCCCGAACC CCAGACTATT 
TCAGCCGGGA CTGAAAAAGA CTTGTTCAAG TTTGAGGAAG ATCTAGATGA TGACATATTC 
CCCAAAAGGT GGAGCCTACC AGTGTTTCGG GAGTCCAGGC ACACCTCCCT AGGAAACTTA 
GGTCCGGATC CTGTGAGTCC AGAGAATGCT CTTTTACTTG ACACAGAAAC ATCCTCAGAG 
ACCAACGGAG TCCATCCTGG GGCTGGCTCT GCCGTCTCTC CCGACATCCT GTATTTAATG 
GAAAGTTTGG ATAAGGAAAC GGACATCCTG GAGTTTAACA ACGAGCACAC TGAAGAACCC 
CTTAAGGGAA CACCGCCGCG CCCCAGGACC TGGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450338.1
CDS	591..2609
Translation	MTVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTGAQKRYFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDQHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTADERKLFDHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTIQMGLSGSDFPAEDWLWNYEKQGHRHSVMRRVKRFLSTHEHPGSPKRRRSFGRQASDSSMFFPPSPARDLLNVPSRNPHRGSPTRKQSHSQEGSPKLHSSMGELSTIRETSRTSTLQSLSQQSSVRASPTKMPQVPEVLITAAEAPEAAFASDNHQRDSTTSILSLEFTGVQASGTEQQEDPSGKVTPPGDPEPQTISAGTEKDLFKFEEDLDDDIFPKRWSLPVFRESRHTSLGNLGPDPVSPENALLLDTETSSETNGVHPGAGSAVSPDILYLMESLDKETDILEFNNEHTEEPLKGTPPRPRTWF

Target ORF information:

RefSeq Version	XM_017594849.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bestrophin 3 (Best3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594849.1

ORF Insert Sequence:

ATGACTGTCA CTTACTCCAG TAAAGTAGCA AATGCGACTT TTTTTGGATT TCATAGGTTG 
CTCCTCAAGT GGAGAGGCAG CATCTACAAA CTGCTGTACA GGGAATTTAT TGTTTTTGCT 
GTTCTTTACA CAGCAATCAG TCTGGTGTAC AGATTGTTAC TTACAGGAGC CCAAAAACGT 
TACTTTGAAA AATTATCAAT TTACTGTGAC AGATATGCTG AACAAATTCC AGTAACCTTT 
GTGCTTGGGT TTTACGTTAC TCTGGTAGTG AACCGATGGT GGAACCAGTT TGTGAATCTG 
CCTTGGCCAG ACAGGCTGAT GTTCCTCATC TCCAGCAGTG TCCACGGGAG CGACCAGCAT 
GGGCGCCTGC TCAGAAGGAC GCTGATGCGC TACGTCAATC TCACGTCCTT GCTCATCTTC 
CGCTCGGTGA GCACAGCGGT GTACAAAAGA TTCCCCACCA TGGACCACGT GGTCGAAGCA 
GGTTTTATGA CGGCAGATGA GAGGAAGTTA TTCGACCACC TCAAGTCGCC TCATCTGAAA 
TACTGGGTTC CGTTCATTTG GTTTGGAAAT CTTGCAACCA AAGCCCGGAA TGAAGGCAGA 
ATCAGAGACA GCGTCGATCT ACAATCACTG ATGACCGAAA TGAATCGGTA CCGCTCTTGG 
TGCAGCCTCT TATTTGGTTA CGACTGGGTT GGCATTCCAC TGGTTTACAC ACAGGTCGTC 
ACTCTCGCCG TCTACACGTT CTTCTTTGCA TGCCTGATCG GACGGCAGTT CCTGGATCCC 
ACCAAAGGCT ACGCGGGACA CGACTTGGAC CTGTACATTC CCATCTTCAC CCTCCTCCAG 
TTCTTCTTCT ACGCAGGATG GCTCAAGGTT GCCGAGCAGC TGATCAACCC TTTCGGGGAA 
GACGACGACG ACTTCGAAAC AAACTGGTGC ATTGACAGAA ACCTGCAGGT CTCTCTTCTG 
GCTGTGGATG AAATGCACAT GAGTTTACCC AAGATGAAGA AGGACATTTA CTGGGACGAC 
TCTGCTGCTC GGCCACCGTA CACGCTGGCA GCGGCTGACT ACTGTATACC TTCCTTCCTG 
GGCTCAACAA TCCAAATGGG GCTGTCCGGG TCCGACTTTC CCGCGGAGGA TTGGCTGTGG 
AACTATGAGA AGCAAGGCCA CCGGCACTCG GTGATGAGGA GGGTCAAGCG TTTCCTGAGT 
ACCCACGAGC ACCCCGGCAG TCCCAAGAGG AGGAGGAGTT TCGGCAGGCA GGCCAGTGAC 
AGCTCCATGT TCTTCCCCCC AAGCCCAGCT CGGGATCTGC TGAATGTGCC CTCAAGGAAC 
CCCCACCGAG GCTCACCTAC CCGGAAGCAA TCCCACTCCC AGGAGGGCAG CCCTAAGCTT 
CACTCCAGCA TGGGAGAATT GTCCACCATC AGGGAGACCA GCCGCACCAG CACTCTGCAG 
AGCCTGAGCC AGCAGTCCAG TGTGAGGGCC TCCCCTACCA AAATGCCCCA GGTCCCCGAG 
GTCCTAATCA CTGCAGCCGA AGCCCCGGAG GCGGCGTTCG CATCAGACAA CCATCAGCGT 
GACTCCACTA CCTCCATCTT GAGCCTCGAG TTCACTGGGG TGCAGGCGAG CGGGACTGAG 
CAGCAGGAAG ACCCCTCGGG GAAGGTGACA CCTCCTGGGG ACCCCGAACC CCAGACTATT 
TCAGCCGGGA CTGAAAAAGA CTTGTTCAAG TTTGAGGAAG ATCTAGATGA TGACATATTC 
CCCAAAAGGT GGAGCCTACC AGTGTTTCGG GAGTCCAGGC ACACCTCCCT AGGAAACTTA 
GGTCCGGATC CTGTGAGTCC AGAGAATGCT CTTTTACTTG ACACAGAAAC ATCCTCAGAG 
ACCAACGGAG TCCATCCTGG GGCTGGCTCT GCCGTCTCTC CCGACATCCT GTATTTAATG 
GAAAGTTTGG ATAAGGAAAC GGACATCCTG GAGTTTAACA ACGAGCACAC TGAAGAACCC 
CTTAAGGGAA CACCGCCGCG CCCCAGGACC TGGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Best3 ( XM_017594848.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450337.1 Rattus norvegicus bestrophin 3 (Best3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47364
Clone ID Related Accession (Same CDS sequence)	XM_017594848.1
Accession Version	XM_017594848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bestrophin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGG CTAAGACCGA AAGCAAACTA AGAACTGAGG CTGTTTATAA CGCTATAACT 
GTCATCAGTG GGCAAACTGG CACAGACAGG CCTCTGCAAG TGGAAAAACA TTTAACCCTG 
GGCTCGCTCG CTACTCGCCG GATTGTCACA GCAGCCGGAG AACAGGAGGA AACCCGCCTG 
GACACGGGTG AAAGGTGGAA GCCGGAGTGC CTCTTTGGGA ACATTCAGAC GAATGAAGAT 
TTTTCTTCCC TTGGGAGACT CCATAGAAAT AGAACAAAAC TATCCAAGAT GACTGTCACT 
TACTCCAGTA AAGTAGCAAA TGCGACTTTT TTTGGATTTC ATAGGTTGCT CCTCAAGTGG 
AGAGGCAGCA TCTACAAACT GCTGTACAGG GAATTTATTG TTTTTGCTGT TCTTTACACA 
GCAATCAGTC TGGTGTACAG ATTGTTACTT ACAGGAGCCC AAAAACGTTA CTTTGAAAAA 
TTATCAATTT ACTGTGACAG ATATGCTGAA CAAATTCCAG TAACCTTTGT GCTTGGGTTT 
TACGTTACTC TGGTAGTGAA CCGATGGTGG AACCAGTTTG TGAATCTGCC TTGGCCAGAC 
AGGCTGATGT TCCTCATCTC CAGCAGTGTC CACGGGAGCG ACCAGCATGG GCGCCTGCTC 
AGAAGGACGC TGATGCGCTA CGTCAATCTC ACGTCCTTGC TCATCTTCCG CTCGGTGAGC 
ACAGCGGTGT ACAAAAGATT CCCCACCATG GACCACGTGG TCGAAGCAGG TTTTATGACG 
GCAGATGAGA GGAAGTTATT CGACCACCTC AAGTCGCCTC ATCTGAAATA CTGGGTTCCG 
TTCATTTGGT TTGGAAATCT TGCAACCAAA GCCCGGAATG AAGGCAGAAT CAGAGACAGC 
GTCGATCTAC AATCACTGAT GACCGAAATG AATCGGTACC GCTCTTGGTG CAGCCTCTTA 
TTTGGTTACG ACTGGGTTGG CATTCCACTG GTTTACACAC AGGTCGTCAC TCTCGCCGTC 
TACACGTTCT TCTTTGCATG CCTGATCGGA CGGCAGTTCC TGGATCCCAC CAAAGGCTAC 
GCGGGACACG ACTTGGACCT GTACATTCCC ATCTTCACCC TCCTCCAGTT CTTCTTCTAC 
GCAGGATGGC TCAAGGTTGC CGAGCAGCTG ATCAACCCTT TCGGGGAAGA CGACGACGAC 
TTCGAAACAA ACTGGTGCAT TGACAGAAAC CTGCAGGTCT CTCTTCTGGC TGTGGATGAA 
ATGCACATGA GTTTACCCAA GATGAAGAAG GACATTTACT GGGACGACTC TGCTGCTCGG 
CCACCGTACA CGCTGGCAGC GGCTGACTAC TGTATACCTT CCTTCCTGGG CTCAACAATC 
CAAATGGGGC TGTCCGGGTC CGACTTTCCC GCGGAGGATT GGCTGTGGAA CTATGAGAAG 
CAAGGCCACC GGCACTCGGT GATGAGGAGG GTCAAGCGTT TCCTGAGTAC CCACGAGCAC 
CCCGGCAGTC CCAAGAGGAG GAGGAGTTTC GGCAGGCAGG CCAGTGACAG CTCCATGTTC 
TTCCCCCCAA GCCCAGCTCG GGATCTGCTG AATGTGCCCT CAAGGAACCC CCACCGAGGC 
TCACCTACCC GGAAGCAATC CCACTCCCAG GAGGGCAGCC CTAAGCTTCA CTCCAGCATG 
GGAGAATTGT CCACCATCAG GGAGACCAGC CGCACCAGCA CTCTGCAGAG CCTGAGCCAG 
CAGTCCAGTG TGAGGGCCTC CCCTACCAAA ATGCCCCAGG TCCCCGAGGT CCTAATCACT 
GCAGCCGAAG CCCCGGAGGC GGCGTTCGCA TCAGACAACC ATCAGCGTGA CTCCACTACC 
TCCATCTTGA GCCTCGAGTT CACTGGGGTG CAGGCGAGCG GGACTGAGCA GCAGGAAGAC 
CCCTCGGGGA AGGTGACACC TCCTGGGGAC CCCGAACCCC AGACTATTTC AGCCGGGACT 
GAAAAAGACT TGTTCAAGTT TGAGGAAGAT CTAGATGATG ACATATTCCC CAAAAGGTGG 
AGCCTACCAG TGTTTCGGGA GTCCAGGCAC ACCTCCCTAG GAAACTTAGG TCCGGATCCT 
GTGAGTCCAG AGAATGCTCT TTTACTTGAC ACAGAAACAT CCTCAGAGAC CAACGGAGTC 
CATCCTGGGG CTGGCTCTGC CGTCTCTCCC GACATCCTGT ATTTAATGGA AAGTTTGGAT 
AAGGAAACGG ACATCCTGGA GTTTAACAAC GAGCACACTG AAGAACCCCT TAAGGGAACA 
CCGCCGCGCC CCAGGACCTG GTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450337.1
CDS	118..2424
Translation	MSQAKTESKLRTEAVYNAITVISGQTGTDRPLQVEKHLTLGSLATRRIVTAAGEQEETRLDTGERWKPECLFGNIQTNEDFSSLGRLHRNRTKLSKMTVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTGAQKRYFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDQHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTADERKLFDHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTIQMGLSGSDFPAEDWLWNYEKQGHRHSVMRRVKRFLSTHEHPGSPKRRRSFGRQASDSSMFFPPSPARDLLNVPSRNPHRGSPTRKQSHSQEGSPKLHSSMGELSTIRETSRTSTLQSLSQQSSVRASPTKMPQVPEVLITAAEAPEAAFASDNHQRDSTTSILSLEFTGVQASGTEQQEDPSGKVTPPGDPEPQTISAGTEKDLFKFEEDLDDDIFPKRWSLPVFRESRHTSLGNLGPDPVSPENALLLDTETSSETNGVHPGAGSAVSPDILYLMESLDKETDILEFNNEHTEEPLKGTPPRPRTWF

Target ORF information:

RefSeq Version	XM_017594848.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bestrophin 3 (Best3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594848.1

ORF Insert Sequence:

ATGAGCCAGG CTAAGACCGA AAGCAAACTA AGAACTGAGG CTGTTTATAA CGCTATAACT 
GTCATCAGTG GGCAAACTGG CACAGACAGG CCTCTGCAAG TGGAAAAACA TTTAACCCTG 
GGCTCGCTCG CTACTCGCCG GATTGTCACA GCAGCCGGAG AACAGGAGGA AACCCGCCTG 
GACACGGGTG AAAGGTGGAA GCCGGAGTGC CTCTTTGGGA ACATTCAGAC GAATGAAGAT 
TTTTCTTCCC TTGGGAGACT CCATAGAAAT AGAACAAAAC TATCCAAGAT GACTGTCACT 
TACTCCAGTA AAGTAGCAAA TGCGACTTTT TTTGGATTTC ATAGGTTGCT CCTCAAGTGG 
AGAGGCAGCA TCTACAAACT GCTGTACAGG GAATTTATTG TTTTTGCTGT TCTTTACACA 
GCAATCAGTC TGGTGTACAG ATTGTTACTT ACAGGAGCCC AAAAACGTTA CTTTGAAAAA 
TTATCAATTT ACTGTGACAG ATATGCTGAA CAAATTCCAG TAACCTTTGT GCTTGGGTTT 
TACGTTACTC TGGTAGTGAA CCGATGGTGG AACCAGTTTG TGAATCTGCC TTGGCCAGAC 
AGGCTGATGT TCCTCATCTC CAGCAGTGTC CACGGGAGCG ACCAGCATGG GCGCCTGCTC 
AGAAGGACGC TGATGCGCTA CGTCAATCTC ACGTCCTTGC TCATCTTCCG CTCGGTGAGC 
ACAGCGGTGT ACAAAAGATT CCCCACCATG GACCACGTGG TCGAAGCAGG TTTTATGACG 
GCAGATGAGA GGAAGTTATT CGACCACCTC AAGTCGCCTC ATCTGAAATA CTGGGTTCCG 
TTCATTTGGT TTGGAAATCT TGCAACCAAA GCCCGGAATG AAGGCAGAAT CAGAGACAGC 
GTCGATCTAC AATCACTGAT GACCGAAATG AATCGGTACC GCTCTTGGTG CAGCCTCTTA 
TTTGGTTACG ACTGGGTTGG CATTCCACTG GTTTACACAC AGGTCGTCAC TCTCGCCGTC 
TACACGTTCT TCTTTGCATG CCTGATCGGA CGGCAGTTCC TGGATCCCAC CAAAGGCTAC 
GCGGGACACG ACTTGGACCT GTACATTCCC ATCTTCACCC TCCTCCAGTT CTTCTTCTAC 
GCAGGATGGC TCAAGGTTGC CGAGCAGCTG ATCAACCCTT TCGGGGAAGA CGACGACGAC 
TTCGAAACAA ACTGGTGCAT TGACAGAAAC CTGCAGGTCT CTCTTCTGGC TGTGGATGAA 
ATGCACATGA GTTTACCCAA GATGAAGAAG GACATTTACT GGGACGACTC TGCTGCTCGG 
CCACCGTACA CGCTGGCAGC GGCTGACTAC TGTATACCTT CCTTCCTGGG CTCAACAATC 
CAAATGGGGC TGTCCGGGTC CGACTTTCCC GCGGAGGATT GGCTGTGGAA CTATGAGAAG 
CAAGGCCACC GGCACTCGGT GATGAGGAGG GTCAAGCGTT TCCTGAGTAC CCACGAGCAC 
CCCGGCAGTC CCAAGAGGAG GAGGAGTTTC GGCAGGCAGG CCAGTGACAG CTCCATGTTC 
TTCCCCCCAA GCCCAGCTCG GGATCTGCTG AATGTGCCCT CAAGGAACCC CCACCGAGGC 
TCACCTACCC GGAAGCAATC CCACTCCCAG GAGGGCAGCC CTAAGCTTCA CTCCAGCATG 
GGAGAATTGT CCACCATCAG GGAGACCAGC CGCACCAGCA CTCTGCAGAG CCTGAGCCAG 
CAGTCCAGTG TGAGGGCCTC CCCTACCAAA ATGCCCCAGG TCCCCGAGGT CCTAATCACT 
GCAGCCGAAG CCCCGGAGGC GGCGTTCGCA TCAGACAACC ATCAGCGTGA CTCCACTACC 
TCCATCTTGA GCCTCGAGTT CACTGGGGTG CAGGCGAGCG GGACTGAGCA GCAGGAAGAC 
CCCTCGGGGA AGGTGACACC TCCTGGGGAC CCCGAACCCC AGACTATTTC AGCCGGGACT 
GAAAAAGACT TGTTCAAGTT TGAGGAAGAT CTAGATGATG ACATATTCCC CAAAAGGTGG 
AGCCTACCAG TGTTTCGGGA GTCCAGGCAC ACCTCCCTAG GAAACTTAGG TCCGGATCCT 
GTGAGTCCAG AGAATGCTCT TTTACTTGAC ACAGAAACAT CCTCAGAGAC CAACGGAGTC 
CATCCTGGGG CTGGCTCTGC CGTCTCTCCC GACATCCTGT ATTTAATGGA AAGTTTGGAT 
AAGGAAACGG ACATCCTGGA GTTTAACAAC GAGCACACTG AAGAACCCCT TAAGGGAACA 
CCGCCGCGCC CCAGGACCTG GTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Best3 ( XM_017594850.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450339.1 Rattus norvegicus bestrophin 3 (Best3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa62012
Clone ID Related Accession (Same CDS sequence)	XM_017594850.1
Accession Version	XM_017594850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bestrophin-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATTT TTCTTCCCTT GGGAGACTCC ATAGAAATAG GTTGCTCCTC AAGTGGAGAG 
GCAGCATCTA CAAACTGCTG TACAGGGAAT TTATTGTTTT TGCTGTTCTT TACACAGCAA 
TCAGTCTGGT GTACAGGGTT TTACGTTACT CTGGTAGTGA ACCGATGGTG GAACCAGTTT 
GTGAATCTGC CTTGGCCAGA CAGGCTGATG TTCCTCATCT CCAGCAGTGT CCACGGGAGC 
GACCAGCATG GGCGCCTGCT CAGAAGGACG CTGATGCGCT ACGTCAATCT CACGTCCTTG 
CTCATCTTCC GCTCGGTGAG CACAGCGGTG TACAAAAGAT TCCCCACCAT GGACCACGTG 
GTCGAAGCAG GTTTTATGAC GGCAGATGAG AGGAAGTTAT TCGACCACCT CAAGTCGCCT 
CATCTGAAAT ACTGGGTTCC GTTCATTTGG TTTGGAAATC TTGCAACCAA AGCCCGGAAT 
GAAGGCAGAA TCAGAGACAG CGTCGATCTA CAATCACTGA TGACCGAAAT GAATCGGTAC 
CGCTCTTGGT GCAGCCTCTT ATTTGGTTAC GACTGGGTTG GCATTCCACT GGTTTACACA 
CAGGTCGTCA CTCTCGCCGT CTACACGTTC TTCTTTGCAT GCCTGATCGG ACGGCAGTTC 
CTGGATCCCA CCAAAGGCTA CGCGGGACAC GACTTGGACC TGTACATTCC CATCTTCACC 
CTCCTCCAGT TCTTCTTCTA CGCAGGATGG CTCAAGGTTG CCGAGCAGCT GATCAACCCT 
TTCGGGGAAG ACGACGACGA CTTCGAAACA AACTGGTGCA TTGACAGAAA CCTGCAGGTC 
TCTCTTCTGG CTGTGGATGA AATGCACATG AGTTTACCCA AGATGAAGAA GGACATTTAC 
TGGGACGACT CTGCTGCTCG GCCACCGTAC ACGCTGGCAG CGGCTGACTA CTGTATACCT 
TCCTTCCTGG GCTCAACAAT CCAAATGGGG CTGTCCGGGT CCGACTTTCC CGCGGAGGAT 
TGGCTGTGGA ACTATGAGAA GCAAGGCCAC CGGCACTCGG TGATGAGGAG GGTCAAGCGT 
TTCCTGAGTA CCCACGAGCA CCCCGGCAGT CCCAAGAGGA GGAGGAGTTT CGGCAGGCAG 
GCCAGTGACA GCTCCATGTT CTTCCCCCCA AGCCCAGCTC GGGATCTGCT GAATGTGCCC 
TCAAGGAACC CCCACCGAGG CTCACCTACC CGGAAGCAAT CCCACTCCCA GGAGGGCAGC 
CCTAAGCTTC ACTCCAGCAT GGGAGAATTG TCCACCATCA GGGAGACCAG CCGCACCAGC 
ACTCTGCAGA GCCTGAGCCA GCAGTCCAGT GTGAGGGCCT CCCCTACCAA AATGCCCCAG 
GTCCCCGAGG TCCTAATCAC TGCAGCCGAA GCCCCGGAGG CGGCGTTCGC ATCAGACAAC 
CATCAGCGTG ACTCCACTAC CTCCATCTTG AGCCTCGAGT TCACTGGGGT GCAGGCGAGC 
GGGACTGAGC AGCAGGAAGA CCCCTCGGGG AAGGTGACAC CTCCTGGGGA CCCCGAACCC 
CAGACTATTT CAGCCGGGAC TGAAAAAGAC TTGTTCAAGT TTGAGGAAGA TCTAGATGAT 
GACATATTCC CCAAAAGGTG GAGCCTACCA GTGTTTCGGG AGTCCAGGCA CACCTCCCTA 
GGAAACTTAG GTCCGGATCC TGTGAGTCCA GAGAATGCTC TTTTACTTGA CACAGAAACA 
TCCTCAGAGA CCAACGGAGT CCATCCTGGG GCTGGCTCTG CCGTCTCTCC CGACATCCTG 
TATTTAATGG AAAGTTTGGA TAAGGAAACG GACATCCTGG AGTTTAACAA CGAGCACACT 
GAAGAACCCC TTAAGGGAAC ACCGCCGCGC CCCAGGACCT GGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450339.1
CDS	94..2001
Translation	MKIFLPLGDSIEIGCSSSGEAASTNCCTGNLLFLLFFTQQSVWCTGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDQHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTADERKLFDHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTIQMGLSGSDFPAEDWLWNYEKQGHRHSVMRRVKRFLSTHEHPGSPKRRRSFGRQASDSSMFFPPSPARDLLNVPSRNPHRGSPTRKQSHSQEGSPKLHSSMGELSTIRETSRTSTLQSLSQQSSVRASPTKMPQVPEVLITAAEAPEAAFASDNHQRDSTTSILSLEFTGVQASGTEQQEDPSGKVTPPGDPEPQTISAGTEKDLFKFEEDLDDDIFPKRWSLPVFRESRHTSLGNLGPDPVSPENALLLDTETSSETNGVHPGAGSAVSPDILYLMESLDKETDILEFNNEHTEEPLKGTPPRPRTWF

Target ORF information:

RefSeq Version	XM_017594850.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bestrophin 3 (Best3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594850.1

ORF Insert Sequence:

ATGAAGATTT TTCTTCCCTT GGGAGACTCC ATAGAAATAG GTTGCTCCTC AAGTGGAGAG 
GCAGCATCTA CAAACTGCTG TACAGGGAAT TTATTGTTTT TGCTGTTCTT TACACAGCAA 
TCAGTCTGGT GTACAGGGTT TTACGTTACT CTGGTAGTGA ACCGATGGTG GAACCAGTTT 
GTGAATCTGC CTTGGCCAGA CAGGCTGATG TTCCTCATCT CCAGCAGTGT CCACGGGAGC 
GACCAGCATG GGCGCCTGCT CAGAAGGACG CTGATGCGCT ACGTCAATCT CACGTCCTTG 
CTCATCTTCC GCTCGGTGAG CACAGCGGTG TACAAAAGAT TCCCCACCAT GGACCACGTG 
GTCGAAGCAG GTTTTATGAC GGCAGATGAG AGGAAGTTAT TCGACCACCT CAAGTCGCCT 
CATCTGAAAT ACTGGGTTCC GTTCATTTGG TTTGGAAATC TTGCAACCAA AGCCCGGAAT 
GAAGGCAGAA TCAGAGACAG CGTCGATCTA CAATCACTGA TGACCGAAAT GAATCGGTAC 
CGCTCTTGGT GCAGCCTCTT ATTTGGTTAC GACTGGGTTG GCATTCCACT GGTTTACACA 
CAGGTCGTCA CTCTCGCCGT CTACACGTTC TTCTTTGCAT GCCTGATCGG ACGGCAGTTC 
CTGGATCCCA CCAAAGGCTA CGCGGGACAC GACTTGGACC TGTACATTCC CATCTTCACC 
CTCCTCCAGT TCTTCTTCTA CGCAGGATGG CTCAAGGTTG CCGAGCAGCT GATCAACCCT 
TTCGGGGAAG ACGACGACGA CTTCGAAACA AACTGGTGCA TTGACAGAAA CCTGCAGGTC 
TCTCTTCTGG CTGTGGATGA AATGCACATG AGTTTACCCA AGATGAAGAA GGACATTTAC 
TGGGACGACT CTGCTGCTCG GCCACCGTAC ACGCTGGCAG CGGCTGACTA CTGTATACCT 
TCCTTCCTGG GCTCAACAAT CCAAATGGGG CTGTCCGGGT CCGACTTTCC CGCGGAGGAT 
TGGCTGTGGA ACTATGAGAA GCAAGGCCAC CGGCACTCGG TGATGAGGAG GGTCAAGCGT 
TTCCTGAGTA CCCACGAGCA CCCCGGCAGT CCCAAGAGGA GGAGGAGTTT CGGCAGGCAG 
GCCAGTGACA GCTCCATGTT CTTCCCCCCA AGCCCAGCTC GGGATCTGCT GAATGTGCCC 
TCAAGGAACC CCCACCGAGG CTCACCTACC CGGAAGCAAT CCCACTCCCA GGAGGGCAGC 
CCTAAGCTTC ACTCCAGCAT GGGAGAATTG TCCACCATCA GGGAGACCAG CCGCACCAGC 
ACTCTGCAGA GCCTGAGCCA GCAGTCCAGT GTGAGGGCCT CCCCTACCAA AATGCCCCAG 
GTCCCCGAGG TCCTAATCAC TGCAGCCGAA GCCCCGGAGG CGGCGTTCGC ATCAGACAAC 
CATCAGCGTG ACTCCACTAC CTCCATCTTG AGCCTCGAGT TCACTGGGGT GCAGGCGAGC 
GGGACTGAGC AGCAGGAAGA CCCCTCGGGG AAGGTGACAC CTCCTGGGGA CCCCGAACCC 
CAGACTATTT CAGCCGGGAC TGAAAAAGAC TTGTTCAAGT TTGAGGAAGA TCTAGATGAT 
GACATATTCC CCAAAAGGTG GAGCCTACCA GTGTTTCGGG AGTCCAGGCA CACCTCCCTA 
GGAAACTTAG GTCCGGATCC TGTGAGTCCA GAGAATGCTC TTTTACTTGA CACAGAAACA 
TCCTCAGAGA CCAACGGAGT CCATCCTGGG GCTGGCTCTG CCGTCTCTCC CGACATCCTG 
TATTTAATGG AAAGTTTGGA TAAGGAAACG GACATCCTGG AGTTTAACAA CGAGCACACT 
GAAGAACCCC TTAAGGGAAC ACCGCCGCGC CCCAGGACCT GGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Best3 ( XM_017594851.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450340.1 Rattus norvegicus bestrophin 3 (Best3), transcript variant X4, mRNA.

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CloneID	ORa62013
Clone ID Related Accession (Same CDS sequence)	XM_017594851.1
Accession Version	XM_017594851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bestrophin-3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACTTT TTTTGGATTT CATAGGTTGC TCCTCAAGTG GAGAGGCAGC ATCTACAAAC 
TGCTGTACAG GGAATTTATT GTTTTTGCTG TTCTTTACAC AGCAATCAGT CTGGTGTACA 
GGGTTTTACG TTACTCTGGT AGTGAACCGA TGGTGGAACC AGTTTGTGAA TCTGCCTTGG 
CCAGACAGGC TGATGTTCCT CATCTCCAGC AGTGTCCACG GGAGCGACCA GCATGGGCGC 
CTGCTCAGAA GGACGCTGAT GCGCTACGTC AATCTCACGT CCTTGCTCAT CTTCCGCTCG 
GTGAGCACAG CGGTGTACAA AAGATTCCCC ACCATGGACC ACGTGGTCGA AGCAGGTTTT 
ATGACGGCAG ATGAGAGGAA GTTATTCGAC CACCTCAAGT CGCCTCATCT GAAATACTGG 
GTTCCGTTCA TTTGGTTTGG AAATCTTGCA ACCAAAGCCC GGAATGAAGG CAGAATCAGA 
GACAGCGTCG ATCTACAATC ACTGATGACC GAAATGAATC GGTACCGCTC TTGGTGCAGC 
CTCTTATTTG GTTACGACTG GGTTGGCATT CCACTGGTTT ACACACAGGT CGTCACTCTC 
GCCGTCTACA CGTTCTTCTT TGCATGCCTG ATCGGACGGC AGTTCCTGGA TCCCACCAAA 
GGCTACGCGG GACACGACTT GGACCTGTAC ATTCCCATCT TCACCCTCCT CCAGTTCTTC 
TTCTACGCAG GATGGCTCAA GGTTGCCGAG CAGCTGATCA ACCCTTTCGG GGAAGACGAC 
GACGACTTCG AAACAAACTG GTGCATTGAC AGAAACCTGC AGGTCTCTCT TCTGGCTGTG 
GATGAAATGC ACATGAGTTT ACCCAAGATG AAGAAGGACA TTTACTGGGA CGACTCTGCT 
GCTCGGCCAC CGTACACGCT GGCAGCGGCT GACTACTGTA TACCTTCCTT CCTGGGCTCA 
ACAATCCAAA TGGGGCTGTC CGGGTCCGAC TTTCCCGCGG AGGATTGGCT GTGGAACTAT 
GAGAAGCAAG GCCACCGGCA CTCGGTGATG AGGAGGGTCA AGCGTTTCCT GAGTACCCAC 
GAGCACCCCG GCAGTCCCAA GAGGAGGAGG AGTTTCGGCA GGCAGGCCAG TGACAGCTCC 
ATGTTCTTCC CCCCAAGCCC AGCTCGGGAT CTGCTGAATG TGCCCTCAAG GAACCCCCAC 
CGAGGCTCAC CTACCCGGAA GCAATCCCAC TCCCAGGAGG GCAGCCCTAA GCTTCACTCC 
AGCATGGGAG AATTGTCCAC CATCAGGGAG ACCAGCCGCA CCAGCACTCT GCAGAGCCTG 
AGCCAGCAGT CCAGTGTGAG GGCCTCCCCT ACCAAAATGC CCCAGGTCCC CGAGGTCCTA 
ATCACTGCAG CCGAAGCCCC GGAGGCGGCG TTCGCATCAG ACAACCATCA GCGTGACTCC 
ACTACCTCCA TCTTGAGCCT CGAGTTCACT GGGGTGCAGG CGAGCGGGAC TGAGCAGCAG 
GAAGACCCCT CGGGGAAGGT GACACCTCCT GGGGACCCCG AACCCCAGAC TATTTCAGCC 
GGGACTGAAA AAGACTTGTT CAAGTTTGAG GAAGATCTAG ATGATGACAT ATTCCCCAAA 
AGGTGGAGCC TACCAGTGTT TCGGGAGTCC AGGCACACCT CCCTAGGAAA CTTAGGTCCG 
GATCCTGTGA GTCCAGAGAA TGCTCTTTTA CTTGACACAG AAACATCCTC AGAGACCAAC 
GGAGTCCATC CTGGGGCTGG CTCTGCCGTC TCTCCCGACA TCCTGTATTT AATGGAAAGT 
TTGGATAAGG AAACGGACAT CCTGGAGTTT AACAACGAGC ACACTGAAGA ACCCCTTAAG 
GGAACACCGC CGCGCCCCAG GACCTGGTTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450340.1
CDS	619..2511
Translation	MRLFLDFIGCSSSGEAASTNCCTGNLLFLLFFTQQSVWCTGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDQHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTADERKLFDHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTIQMGLSGSDFPAEDWLWNYEKQGHRHSVMRRVKRFLSTHEHPGSPKRRRSFGRQASDSSMFFPPSPARDLLNVPSRNPHRGSPTRKQSHSQEGSPKLHSSMGELSTIRETSRTSTLQSLSQQSSVRASPTKMPQVPEVLITAAEAPEAAFASDNHQRDSTTSILSLEFTGVQASGTEQQEDPSGKVTPPGDPEPQTISAGTEKDLFKFEEDLDDDIFPKRWSLPVFRESRHTSLGNLGPDPVSPENALLLDTETSSETNGVHPGAGSAVSPDILYLMESLDKETDILEFNNEHTEEPLKGTPPRPRTWF

Target ORF information:

RefSeq Version	XM_017594851.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bestrophin 3 (Best3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594851.1

ORF Insert Sequence:

ATGCGACTTT TTTTGGATTT CATAGGTTGC TCCTCAAGTG GAGAGGCAGC ATCTACAAAC 
TGCTGTACAG GGAATTTATT GTTTTTGCTG TTCTTTACAC AGCAATCAGT CTGGTGTACA 
GGGTTTTACG TTACTCTGGT AGTGAACCGA TGGTGGAACC AGTTTGTGAA TCTGCCTTGG 
CCAGACAGGC TGATGTTCCT CATCTCCAGC AGTGTCCACG GGAGCGACCA GCATGGGCGC 
CTGCTCAGAA GGACGCTGAT GCGCTACGTC AATCTCACGT CCTTGCTCAT CTTCCGCTCG 
GTGAGCACAG CGGTGTACAA AAGATTCCCC ACCATGGACC ACGTGGTCGA AGCAGGTTTT 
ATGACGGCAG ATGAGAGGAA GTTATTCGAC CACCTCAAGT CGCCTCATCT GAAATACTGG 
GTTCCGTTCA TTTGGTTTGG AAATCTTGCA ACCAAAGCCC GGAATGAAGG CAGAATCAGA 
GACAGCGTCG ATCTACAATC ACTGATGACC GAAATGAATC GGTACCGCTC TTGGTGCAGC 
CTCTTATTTG GTTACGACTG GGTTGGCATT CCACTGGTTT ACACACAGGT CGTCACTCTC 
GCCGTCTACA CGTTCTTCTT TGCATGCCTG ATCGGACGGC AGTTCCTGGA TCCCACCAAA 
GGCTACGCGG GACACGACTT GGACCTGTAC ATTCCCATCT TCACCCTCCT CCAGTTCTTC 
TTCTACGCAG GATGGCTCAA GGTTGCCGAG CAGCTGATCA ACCCTTTCGG GGAAGACGAC 
GACGACTTCG AAACAAACTG GTGCATTGAC AGAAACCTGC AGGTCTCTCT TCTGGCTGTG 
GATGAAATGC ACATGAGTTT ACCCAAGATG AAGAAGGACA TTTACTGGGA CGACTCTGCT 
GCTCGGCCAC CGTACACGCT GGCAGCGGCT GACTACTGTA TACCTTCCTT CCTGGGCTCA 
ACAATCCAAA TGGGGCTGTC CGGGTCCGAC TTTCCCGCGG AGGATTGGCT GTGGAACTAT 
GAGAAGCAAG GCCACCGGCA CTCGGTGATG AGGAGGGTCA AGCGTTTCCT GAGTACCCAC 
GAGCACCCCG GCAGTCCCAA GAGGAGGAGG AGTTTCGGCA GGCAGGCCAG TGACAGCTCC 
ATGTTCTTCC CCCCAAGCCC AGCTCGGGAT CTGCTGAATG TGCCCTCAAG GAACCCCCAC 
CGAGGCTCAC CTACCCGGAA GCAATCCCAC TCCCAGGAGG GCAGCCCTAA GCTTCACTCC 
AGCATGGGAG AATTGTCCAC CATCAGGGAG ACCAGCCGCA CCAGCACTCT GCAGAGCCTG 
AGCCAGCAGT CCAGTGTGAG GGCCTCCCCT ACCAAAATGC CCCAGGTCCC CGAGGTCCTA 
ATCACTGCAG CCGAAGCCCC GGAGGCGGCG TTCGCATCAG ACAACCATCA GCGTGACTCC 
ACTACCTCCA TCTTGAGCCT CGAGTTCACT GGGGTGCAGG CGAGCGGGAC TGAGCAGCAG 
GAAGACCCCT CGGGGAAGGT GACACCTCCT GGGGACCCCG AACCCCAGAC TATTTCAGCC 
GGGACTGAAA AAGACTTGTT CAAGTTTGAG GAAGATCTAG ATGATGACAT ATTCCCCAAA 
AGGTGGAGCC TACCAGTGTT TCGGGAGTCC AGGCACACCT CCCTAGGAAA CTTAGGTCCG 
GATCCTGTGA GTCCAGAGAA TGCTCTTTTA CTTGACACAG AAACATCCTC AGAGACCAAC 
GGAGTCCATC CTGGGGCTGG CTCTGCCGTC TCTCCCGACA TCCTGTATTT AATGGAAAGT 
TTGGATAAGG AAACGGACAT CCTGGAGTTT AACAACGAGC ACACTGAAGA ACCCCTTAAG 
GGAACACCGC CGCGCCCCAG GACCTGGTTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondria dependent pathway is involved in the protective effect of bestrophin-3 on hydrogen peroxide-induced apoptosis in basilar artery smooth muscle cells.
Apoptosis : an international journal on programmed cell death18(5)556-65(2013 May)
Jiang L,Liu Y,Ma MM,Tang YB,Zhou JG,Guan YY

ERK1/2-dependent bestrophin-3 expression prevents ER-stress-induced cell death in renal epithelial cells by reducing CHOP.
Biochimica et biophysica acta1823(10)1864-76(2012 Oct)
Lee WK,Chakraborty PK,Roussa E,Wolff NA,Th?venod F

Bestrophin is important for the rhythmic but not the tonic contraction in rat mesenteric small arteries.
Cardiovascular research91(4)685-93(2011 Sep)
Broegger T,Jacobsen JC,Secher Dam V,Boedtkjer DM,Kold-Petersen H,Pedersen FS,Aalkjaer C,Matchkov VV

Bestrophin-3 (vitelliform macular dystrophy 2-like 3 protein) is essential for the cGMP-dependent calcium-activated chloride conductance in vascular smooth muscle cells.
Circulation research103(8)864-72(2008 Oct)
Matchkov VV,Larsen P,Bouzinova EV,Rojek A,Boedtkjer DM,Golubinskaya V,Pedersen FS,Aalkjaer C,Nilsson H

A short motif in the C-terminus of mouse bestrophin 3 [corrected] inhibits its activation as a Cl channel.
FEBS letters580(8)2141-6(2006 Apr)
Qu Z,Cui Y,Hartzell C

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Best3 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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