Lrat cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lrat
Entrez Gene ID	64047
Full Name	lecithin retinol acyltransferase
General protein information	Preferred Names lecithin retinol acyltransferase Names lecithin retinol acyltransferase lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase) phosphatidylcholine--retinol O-acyltransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	catalyzes the transfer of the sn-1 fatty acid of phosphatidylcholine to retinol bound to a cellular retinol-binding protein; may function to divert retinol into storage during vitamin A adequacy [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022280.2	NP_071616.1	lecithin retinol acyltransferase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LRAT	XP_539777.2
Gallus gallus (chicken)	LRAT	XP_420371.2
Xenopus tropicalis (tropical clawed frog)	lrat	NP_989141.1
Bos taurus (cattle)	LRAT	NP_803469.1
Rattus norvegicus (Norway rat)	Lrat	NP_071616.1
Danio rerio (zebrafish)	lrata	NP_001191060.1
	LRAT	XP_001092118.1
Xenopus tropicalis (tropical clawed frog)	LOC100485002	XP_002933533.1
Homo sapiens (human)	LRAT	NP_004735.2
Pan troglodytes (chimpanzee)	LRAT	XP_526710.2
Mus musculus (house mouse)	Lrat	NP_076113.1
Danio rerio (zebrafish)	lratb	XP_001921190.2

Gene Ontology
Bibliography

Related articles in PubMed

Genes and mutations causing retinitis pigmentosa.
Daiger SP, Sullivan LS, Bowne SJ
Clinical genetics84(2)132-41(2013 Aug)

Liver retinol transporter and receptor for serum retinol-binding protein (RBP4).
Alapatt P, Guo F, Komanetsky SM, Wang S, Cai J, Sargsyan A, Rodr?guez D?az E, Bacon BT, Aryal P, Graham TE
The Journal of biological chemistry288(2)1250-65(2013 Jan)

Impact of lactobacilli on orally acquired listeriosis.
Archambaud C, Nahori MA, Soubigou G, B?cavin C, Laval L, Lechat P, Smokvina T, Langella P, Lecuit M, Cossart P
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)

Mechanisms involved in the intestinal absorption of dietary vitamin A and provitamin A carotenoids.
Harrison EH
Biochimica et biophysica acta1821(1)70-7(2012 Jan)

Physiological insights into all-trans-retinoic acid biosynthesis.
Napoli JL
Biochimica et biophysica acta1821(1)152-67(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In obese and dyslipidemic fa/fa rats, intestinal beta-Carotene 15,15'-Monooxygenase gene expression was increased, whereas hepatic lecithin:retinol acyltransferase gene expression was deceased.
Title: Altered expression of both ?-carotene 15,15' monooxygenase and lecithin:retinol acyltransferase in obese Zucker rats.

proximal region together with basal transcription factors may be sufficient to drive Lrat expression.
Title: An essential set of basic DNA response elements is required for receptor-dependent transcription of the lecithin:retinol acyltransferase (Lrat) gene.

The results show that the major 5.3 kb transcript uses the canonical signal AAUAAA located at nt 5308, and the major short transcript of approximately 1.5 kb uses the non-canonical signal AUUAAA located at nt 1330.
Title: Cloning and molecular expression analysis of large and small lecithin:retinol acyltransferase mRNAs in the liver and other tissues of adult rats.

The following Lrat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lrat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05612	NM_022280.2 Latest version!	Rattus norvegicus lecithin retinol acyltransferase (Lrat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lrat ( NM_022280.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071616.1 Rattus norvegicus lecithin retinol acyltransferase (Lrat), mRNA.

Price & Availability↑

CloneID	ORa05612
Clone ID Related Accession (Same CDS sequence)	NM_022280.2
Accession Version	NM_022280.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	lecithin retinol acyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF255060.2. On or before Jan 12, 2005 this sequence version replaced XM_213090.1, NM_022280.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF255060.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAAGAACT CAATGCTGGA GGCTGCGTCC CTCCTTCTGG AGAAGCTGCT CCTTATTTCC 
AACTTCAAGA TCTTTAGCGT GTGCGCCCCG GGAGGAGGCA CAGGGAAGAA ACATCCCTAT 
GAAATCAACT CTTTTCTCCG GGGTGACGTG TTGGAAGTGT CACGGACCCA TTTTACCCAC 
TATGGGATCT ACCTGGGGGA CAACCGTGTC GCCCATCTAA TGCCTGACAT CCTGTTGGCC 
CTGACCAGTG ACAAGGAACG CACTCAGAAG GTGGTCTCCA ACAAGCGTCT CCTCCCAGGA 
GTCATTTGCA AGGTGGCCAG CATCCGTGTG GACACAGTAG AGGACTTTGC CTATGGAGCG 
GACATCCTCG TCAATCACCT AGACGAGACT CTCAAGAAGA AGTCCTTGCT CAATGAGGAG 
GTGGCACGCA GAGCAGAGCA GCAGTTGGGG CTGACCCCCT ACAGCCTACT GTGGAACAAC 
TGCGAACACT TTGTGACCTA CTGCAGATAC GGCTCTCCTA TCAGTCCGCA GGCTGAGAAG 
TTTCACGAGA CTGTGAAGAT ACTCATTCGT GATCAGAGAA GTTGTCTTGC TTCAGCTGTC 
TTGGGATTAG TGTCTATTAT CTACACAGGC CTGGCATCAT ATATGACCCT TCCTGCAGTC 
TGCATCCCGT TCTGCTTGTG GATGATGTCT GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071616.1
CDS	271..966
Translation	MKNSMLEAASLLLEKLLLISNFKIFSVCAPGGGTGKKHPYEINSFLRGDVLEVSRTHFTHYGIYLGDNRVAHLMPDILLALTSDKERTQKVVSNKRLLPGVICKVASIRVDTVEDFAYGADILVNHLDETLKKKSLLNEEVARRAEQQLGLTPYSLLWNNCEHFVTYCRYGSPISPQAEKFHETVKILIRDQRSCLASAVLGLVSIIYTGLASYMTLPAVCIPFCLWMMSG

Target ORF information:

RefSeq Version	NM_022280.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lecithin retinol acyltransferase (Lrat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022280.2

ORF Insert Sequence:

ATGAAGAACT CAATGCTGGA GGCTGCGTCC CTCCTTCTGG AGAAGCTGCT CCTTATTTCC 
AACTTCAAGA TCTTTAGCGT GTGCGCCCCG GGAGGAGGCA CAGGGAAGAA ACATCCCTAT 
GAAATCAACT CTTTTCTCCG GGGTGACGTG TTGGAAGTGT CACGGACCCA TTTTACCCAC 
TATGGGATCT ACCTGGGGGA CAACCGTGTC GCCCATCTAA TGCCTGACAT CCTGTTGGCC 
CTGACCAGTG ACAAGGAACG CACTCAGAAG GTGGTCTCCA ACAAGCGTCT CCTCCCAGGA 
GTCATTTGCA AGGTGGCCAG CATCCGTGTG GACACAGTAG AGGACTTTGC CTATGGAGCG 
GACATCCTCG TCAATCACCT AGACGAGACT CTCAAGAAGA AGTCCTTGCT CAATGAGGAG 
GTGGCACGCA GAGCAGAGCA GCAGTTGGGG CTGACCCCCT ACAGCCTACT GTGGAACAAC 
TGCGAACACT TTGTGACCTA CTGCAGATAC GGCTCTCCTA TCAGTCCGCA GGCTGAGAAG 
TTTCACGAGA CTGTGAAGAT ACTCATTCGT GATCAGAGAA GTTGTCTTGC TTCAGCTGTC 
TTGGGATTAG TGTCTATTAT CTACACAGGC CTGGCATCAT ATATGACCCT TCCTGCAGTC 
TGCATCCCGT TCTGCTTGTG GATGATGTCT GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genes and mutations causing retinitis pigmentosa.
Clinical genetics84(2)132-41(2013 Aug)
Daiger SP,Sullivan LS,Bowne SJ

Liver retinol transporter and receptor for serum retinol-binding protein (RBP4).
The Journal of biological chemistry288(2)1250-65(2013 Jan)
Alapatt P,Guo F,Komanetsky SM,Wang S,Cai J,Sargsyan A,Rodr?guez D?az E,Bacon BT,Aryal P,Graham TE

Impact of lactobacilli on orally acquired listeriosis.
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)
Archambaud C,Nahori MA,Soubigou G,B?cavin C,Laval L,Lechat P,Smokvina T,Langella P,Lecuit M,Cossart P

Mechanisms involved in the intestinal absorption of dietary vitamin A and provitamin A carotenoids.
Biochimica et biophysica acta1821(1)70-7(2012 Jan)
Harrison EH

Physiological insights into all-trans-retinoic acid biosynthesis.
Biochimica et biophysica acta1821(1)152-67(2012 Jan)
Napoli JL