Ccn4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ccn4
Entrez Gene ID	65154
Full Name	WNT1 inducible signaling pathway protein 1
General protein information	Preferred Names WNT1 inducible signaling pathway protein 1 Names WNT1-inducible-signaling pathway protein 1 CCN family member 4 ELM-1 WISP-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34
Summary	human homolog is overexpressed in scirrhous gastric carcinomas; may play a role in transformation and tumor invasion [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031716.1	NP_113904.1	WNT1-inducible-signaling pathway protein 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	WISP1	XP_528234.2
Bos taurus (cattle)	WISP1	XP_002692649.2
Mus musculus (house mouse)	Wisp1	NP_061353.1
Gallus gallus (chicken)	WISP1	NP_001019750.1
	WISP1	XP_001087371.1
Danio rerio (zebrafish)	wisp1a	NP_001159702.1
Danio rerio (zebrafish)	wisp1b	NP_001107100.1
Homo sapiens (human)	WISP1	NP_003873.1
Canis lupus familiaris (dog)	WISP1	XP_005628355.1
Rattus norvegicus (Norway rat)	Wisp1	NP_113904.1
Xenopus tropicalis (tropical clawed frog)	wisp1	XP_002939562.2

Gene Ontology
Bibliography

Related articles in PubMed

WISP1 promotes non-alcoholic fatty liver disease and skeletal muscle insulin resistance via TLR4/JNK signaling.
Jung TW, Kang C, Goh J, Chae SI, Kim HC, Lee TJ, El-Aty AMA, Jeong JH
Journal of cellular physiology(2018 Jan)

CCN4/WISP1 controls cutaneous wound healing by modulating proliferation, migration and ECM expression in dermal fibroblasts via ?5?1 and TNF?.
Ono M, Masaki A, Maeda A, Kilts TM, Hara ES, Komori T, Pham H, Kuboki T, Young MF
Matrix biology : journal of the International Society for Matrix Biology(2018 Jan)

WISP1/CCN4 inhibits adipocyte differentiation through repression of PPAR? activity.
Ferrand N, B?reziat V, Moldes M, Zaoui M, Larsen AK, Sabbah M
Scientific reports7(1)1749(2017 May)

Thymosin ?4 Prevents Angiotensin II-Induced Cardiomyocyte Growth by Regulating Wnt/WISP Signaling.
Li L, Guleria RS, Thakur S, Zhang CL, Pan J, Baker KM, Gupta S
Journal of cellular physiology231(8)1737-44(2016 Aug)

Functional Effects of WNT1-Inducible Signaling Pathway Protein-1 on Bronchial Smooth Muscle Cell Migration and Proliferation in OVA-Induced Airway Remodeling.
Yang M, Du Y, Xu Z, Jiang Y
Inflammation39(1)16-29(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thymosin beta4 targets Wnt/WISP-1 signaling to protect against Ang II-induced cardiomyocyte growth.
Title: Thymosin ?4 Prevents Angiotensin II-Induced Cardiomyocyte Growth by Regulating Wnt/WISP Signaling.

WISP1 may be a potential inducer of airway smooth muscle proliferation and hypertrophy in asthma. The pro-remodeling effect of WISP1 is likely due to be involved in PI3K-GSK3beta-dependent noncanonical TGF-beta signaling.
Title: Functional Effects of WNT1-Inducible Signaling Pathway Protein-1 on Bronchial Smooth Muscle Cell Migration and Proliferation in OVA-Induced Airway Remodeling.

these results showed the upregulation of WISP-1 in both in vitro and in vivo liver fibrosis models, and WISP-1 stimulated the proliferation of HSCs in vitro.
Title: Wnt-induced secreted protein 1/CCN4 in liver fibrosis both in vitro and in vivo.

initiate and perpetuate the pathological remodeling of asthma by inducing fibroblast proliferation and ECM deposition
Title: A role for WNT1-inducible signaling protein-1 in airway remodeling in a rat asthma model.

Results suggest that CCN4 is involved in the migration and proliferation of vascular smooth muscle cells. Inhibition of CCN4 may provide a promising strategy for the prevention of restenosis after vascular interventions.
Title: CCN4 regulates vascular smooth muscle cell migration and proliferation.

The following Ccn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ccn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13127	NM_031716.1 Latest version!	Rattus norvegicus cellular communication network factor 4 (Ccn4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ccn4 ( NM_031716.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113904.1 Rattus norvegicus cellular communication network factor 4 (Ccn4), mRNA.

Price & Availability↑

CloneID	ORa13127
Clone ID Related Accession (Same CDS sequence)	NM_031716.1
Accession Version	NM_031716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	WNT1-inducible-signaling pathway protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF228049.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF228049.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGGTGGC TCCTGCCCTG GACGCTGGCA GCCGTGGCAG TCCTGATGGT GGGCAACATC 
CTGGCCACGG CCCTCTCTCC AACCCCCACA ACCATGACCT TCACCCCAGC ACCACTGGAG 
GAAACGATTA CACGGCCGGA ATTCTGCAAG TGGCCGTGTG AGTGCCCACA AGCCCCACCT 
CGCTGCCCAC TGGGTGTCAG CCTAATCACA GATGGCTGTG AATGCTGTAA GATATGTGCC 
CAGCAGCTTG GGGACAACTG CACAGAGGCT GCCGTCTGTG ACCCACACCG GGGCCTCTAC 
TGCGATTACA GTGGGGACCG CCCGAGGTAC GCAATAGGAG TGTGTGCACA GGTGGTCGGT 
GTGGGCTGTG TCCTGGATGG CGTGCGCTAC ACCAATGGAG AGTCCTTCCA ACCCAACTGC 
AGGTACAACT GCACCTGTAT TGATGGCACC GTGGGCTGCA CACCACTGTG CCTGAGCCCC 
AGGCCCCCAC GCCTCTGGTG CCGCCAGCCC CGTCACGTGA GAGTCCCTGG CCAGTGCTGT 
GAACAATGGG TATGTGATGA TGATGCCAGG AGGCCACGCC AGACTGCACT GCTGGACACC 
AGAGCCTTTG CAGCTTCGGG TGCAGTGGAG CAACGGTATG AGAACTGCAT AGCCTACACT 
AGCCCCTGGA GTCCCTGCTC AACAACCTGT GGCCTAGGTA TCTCGACTCG CATCTCTAAT 
GTCAATGCGC GGTGCTGGCC AGAGCAGGAA AGTCGCCTCT GCAACCTGCG GCCATGTGAT 
GTGGACATCC GACCACACAT CAAGGCAGGG AAGAAATGCC TGGCTGTGTA CCAGCCAGAG 
GAGGCCACGA ACTTCACTCT CGCAGGCTGT GTCAGCACAC GCACCTACCG ACCCAAGTAC 
TGTGGAGTCT GTACTGACAA TAGGTGTTGC ATCCCCTACA AGTCTAAGAC CATCAGTGTT 
GATTTCCAGT GTCCAGAGGG GCCGGGTTTC TCTCGGCAGG TCCTATGGAT TAATGCTTGC 
TTCTGCAACC TGAGCTGCAG GAATCCTAAT GATATCTTTG CTGACTTGGA ATCTTACCCT 
GACTTTGCAG AGATTGCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113904.1
CDS	164..1267
Translation	MRWLLPWTLAAVAVLMVGNILATALSPTPTTMTFTPAPLEETITRPEFCKWPCECPQAPPRCPLGVSLITDGCECCKICAQQLGDNCTEAAVCDPHRGLYCDYSGDRPRYAIGVCAQVVGVGCVLDGVRYTNGESFQPNCRYNCTCIDGTVGCTPLCLSPRPPRLWCRQPRHVRVPGQCCEQWVCDDDARRPRQTALLDTRAFAASGAVEQRYENCIAYTSPWSPCSTTCGLGISTRISNVNARCWPEQESRLCNLRPCDVDIRPHIKAGKKCLAVYQPEEATNFTLAGCVSTRTYRPKYCGVCTDNRCCIPYKSKTISVDFQCPEGPGFSRQVLWINACFCNLSCRNPNDIFADLESYPDFAEIAN

Target ORF information:

RefSeq Version	NM_031716.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cellular communication network factor 4 (Ccn4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031716.1

ORF Insert Sequence:

ATGAGGTGGC TCCTGCCCTG GACGCTGGCA GCCGTGGCAG TCCTGATGGT GGGCAACATC 
CTGGCCACGG CCCTCTCTCC AACCCCCACA ACCATGACCT TCACCCCAGC ACCACTGGAG 
GAAACGATTA CACGGCCGGA ATTCTGCAAG TGGCCGTGTG AGTGCCCACA AGCCCCACCT 
CGCTGCCCAC TGGGTGTCAG CCTAATCACA GATGGCTGTG AATGCTGTAA GATATGTGCC 
CAGCAGCTTG GGGACAACTG CACAGAGGCT GCCGTCTGTG ACCCACACCG GGGCCTCTAC 
TGCGATTACA GTGGGGACCG CCCGAGGTAC GCAATAGGAG TGTGTGCACA GGTGGTCGGT 
GTGGGCTGTG TCCTGGATGG CGTGCGCTAC ACCAATGGAG AGTCCTTCCA ACCCAACTGC 
AGGTACAACT GCACCTGTAT TGATGGCACC GTGGGCTGCA CACCACTGTG CCTGAGCCCC 
AGGCCCCCAC GCCTCTGGTG CCGCCAGCCC CGTCACGTGA GAGTCCCTGG CCAGTGCTGT 
GAACAATGGG TATGTGATGA TGATGCCAGG AGGCCACGCC AGACTGCACT GCTGGACACC 
AGAGCCTTTG CAGCTTCGGG TGCAGTGGAG CAACGGTATG AGAACTGCAT AGCCTACACT 
AGCCCCTGGA GTCCCTGCTC AACAACCTGT GGCCTAGGTA TCTCGACTCG CATCTCTAAT 
GTCAATGCGC GGTGCTGGCC AGAGCAGGAA AGTCGCCTCT GCAACCTGCG GCCATGTGAT 
GTGGACATCC GACCACACAT CAAGGCAGGG AAGAAATGCC TGGCTGTGTA CCAGCCAGAG 
GAGGCCACGA ACTTCACTCT CGCAGGCTGT GTCAGCACAC GCACCTACCG ACCCAAGTAC 
TGTGGAGTCT GTACTGACAA TAGGTGTTGC ATCCCCTACA AGTCTAAGAC CATCAGTGTT 
GATTTCCAGT GTCCAGAGGG GCCGGGTTTC TCTCGGCAGG TCCTATGGAT TAATGCTTGC 
TTCTGCAACC TGAGCTGCAG GAATCCTAAT GATATCTTTG CTGACTTGGA ATCTTACCCT 
GACTTTGCAG AGATTGCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

WISP1 promotes non-alcoholic fatty liver disease and skeletal muscle insulin resistance via TLR4/JNK signaling.
Journal of cellular physiology(2018 Jan)
Jung TW,Kang C,Goh J,Chae SI,Kim HC,Lee TJ,El-Aty AMA,Jeong JH

CCN4/WISP1 controls cutaneous wound healing by modulating proliferation, migration and ECM expression in dermal fibroblasts via ?5?1 and TNF?.
Matrix biology : journal of the International Society for Matrix Biology(2018 Jan)
Ono M,Masaki A,Maeda A,Kilts TM,Hara ES,Komori T,Pham H,Kuboki T,Young MF

WISP1/CCN4 inhibits adipocyte differentiation through repression of PPAR? activity.
Scientific reports7(1)1749(2017 May)
Ferrand N,B?reziat V,Moldes M,Zaoui M,Larsen AK,Sabbah M

Thymosin ?4 Prevents Angiotensin II-Induced Cardiomyocyte Growth by Regulating Wnt/WISP Signaling.
Journal of cellular physiology231(8)1737-44(2016 Aug)
Li L,Guleria RS,Thakur S,Zhang CL,Pan J,Baker KM,Gupta S

Functional Effects of WNT1-Inducible Signaling Pathway Protein-1 on Bronchial Smooth Muscle Cell Migration and Proliferation in OVA-Induced Airway Remodeling.
Inflammation39(1)16-29(2016 Feb)
Yang M,Du Y,Xu Z,Jiang Y

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Ccn4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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