Cyb5b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyb5b
Entrez Gene ID	80773
Full Name	cytochrome b5 type B
Synonyms	Cyb5m,omb5
General protein information	Preferred Names cytochrome b5 type B Names cytochrome b5 type B cytochrome b5 outer mitochondrial membrane isoform cytochrome b5 type B (outer mitochondrial membrane)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12
Summary	a cytochrome with isoforms located in endoplasmic reticulum and mitochondrial outer membranes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_030586.2	NP_085075.1	cytochrome b5 type B precursor

Pathways from BioSystems

Homologies

	CYB5B	XP_001100954.1
Rattus norvegicus (Norway rat)	Cyb5b	NP_085075.1
Homo sapiens (human)	CYB5B	NP_085056.2
Pan troglodytes (chimpanzee)	CYB5B	NP_001229538.1
Schizosaccharomyces pombe (fission yeast)	oca8	NP_587997.1
Arabidopsis thaliana (thale cress)	CB5-D	NP_199692.1
Danio rerio (zebrafish)	cyb5b	NP_998041.1
Caenorhabditis elegans (roundworm)	cytb-5.2	NP_491931.1
Eremothecium gossypii	AGOS_AFL223W	NP_985327.1
Bos taurus (cattle)	CYB5B	NP_001157254.1
Gallus gallus (chicken)	CYB5B	NP_001025752.1
Kluyveromyces lactis	KLLA0F23672g	XP_456135.1
Magnaporthe oryzae (rice blast fungus)	MGG_06510	XP_003717049.1
Xenopus tropicalis (tropical clawed frog)	cyb5b	NP_001011009.2
Mus musculus (house mouse)	Cyb5b	NP_079834.2
Saccharomyces cerevisiae (baker's yeast)	CYB5	NP_014288.3
Neurospora crassa	NCU03910	XP_957549.2

Gene Ontology
Bibliography

Related articles in PubMed

Amidoxime reductase system containing cytochrome b5 type B (CYB5B) and MOSC2 is of importance for lipid synthesis in adipocyte mitochondria.
Neve EP, Nordling A, Andersson TB, Hellman U, Diczfalusy U, Johansson I, Ingelman-Sundberg M
The Journal of biological chemistry287(9)6307-17(2012 Feb)

Accommodating a nonconservative internal mutation by water-mediated hydrogen bonding between ?-sheet strands: a comparison of human and rat type B (mitochondrial) cytochrome b5.
Parthasarathy S, Altuve A, Terzyan S, Zhang X, Kuczera K, Rivera M, Benson DR
Biochemistry50(24)5544-54(2011 Jun)

Biochemical and spectroscopic characterization of the human mitochondrial amidoxime reducing components hmARC-1 and hmARC-2 suggests the existence of a new molybdenum enzyme family in eukaryotes.
Wahl B, Reichmann D, Niks D, Krompholz N, Havemeyer A, Clement B, Messerschmidt T, Rothkegel M, Biester H, Hille R, Mendel RR, Bittner F
The Journal of biological chemistry285(48)37847-59(2010 Nov)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Influence of point mutations on the flexibility of cytochrome b5: molecular dynamics simulations of holoproteins.
Cheng Q, Benson DR, Rivera M, Kuczera K
Biopolymers83(3)297-312(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The presence of a novel reductive enzyme system of importance for lipid synthesis that is exclusively localized to the outer mitochondrial membrane and is composed of CYB5B, MOSC2, and a third unknown component (a CYB5B reductase).
Title: Amidoxime reductase system containing cytochrome b5 type B (CYB5B) and MOSC2 is of importance for lipid synthesis in adipocyte mitochondria.

comparison of X-ray crystal structures of human and rat CYB5B reveals a striking difference in packing involving the five-strand beta-sheet, which can be attributed to fully buried residue 21 in strand beta4
Title: Accommodating a nonconservative internal mutation by water-mediated hydrogen bonding between ?-sheet strands: a comparison of human and rat type B (mitochondrial) cytochrome b5.

simulations provided qualitative microscopic explanations of many of the differences in physical properties between outer mitochondrial membrane CYB5 isoform and microsomal CYB5 and two mutants in terms of localized changes in structure and flexibility
Title: Influence of point mutations on the flexibility of cytochrome b5: molecular dynamics simulations of holoproteins.

OMb, present abundantly in zona glomerulosa (zG), participates in aldosterone formation in zG of rat under angiotensin II-stimulation through regeneration of ascorbic acid.
Title: Possible participation of outer mitochondrial membrane cytonchrome B5 in steroidogenesis in zona glomerulosa of rat adrenal cortex.

Molecular dynamics simulation studies of this protein were made and some of its physical properties described.
Title: Molecular dynamics simulation studies of cytochrome b5 from outer mitochondrial and microsomal membrane.

The following Cyb5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyb5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13056	NM_030586.2 Latest version!	Rattus norvegicus cytochrome b5 type B (Cyb5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyb5b ( NM_030586.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_085075.1 Rattus norvegicus cytochrome b5 type B (Cyb5b), mRNA.

Price & Availability↑

CloneID	ORa13056
Clone ID Related Accession (Same CDS sequence)	NM_030586.2
Accession Version	NM_030586.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome b5 type B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC072535.1. On Jun 20, 2004 this sequence version replaced NM_030586.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC072535.1, EV775749.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGACCC CAGAGGCCAG CGGCAGCGGC AGGAATGGCC AGGGCTCCGA CCCCGCCGTC 
ACCTACTACC GGCTGGAGGA GGTGGCGAAG CGAAATACCG CGGAGGAGAC GTGGATGGTG 
ATCCACGGGC GAGTCTACGA TATCACCCGC TTCCTCAGCG AGCATCCTGG TGGAGAAGAG 
GTTCTACTGG AACAAGCTGG TGCCGATGCA ACTGAAAGCT TTGAAGATGT CGGCCACTCC 
CCTGATGCCA GGGAGATGCT AAAGCAGTAC TACATTGGTG ATGTCCATCC GAATGACCTT 
AAACCTAAAG ATGGTGACAA GGACCCTTCA AAAAACAATT CATGCCAAAG TTCCTGGGCA 
TATTGGATTG TCCCCATCGT GGGTGCTATT CTTATAGGTT TCCTGTATCG TCACTTCTGG 
GCTGACAGCA AATCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_085075.1
CDS	7..447
Translation	MATPEASGSGRNGQGSDPAVTYYRLEEVAKRNTAEETWMVIHGRVYDITRFLSEHPGGEEVLLEQAGADATESFEDVGHSPDAREMLKQYYIGDVHPNDLKPKDGDKDPSKNNSCQSSWAYWIVPIVGAILIGFLYRHFWADSKSS

Target ORF information:

RefSeq Version	NM_030586.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome b5 type B (Cyb5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030586.2

ORF Insert Sequence:

ATGGCGACCC CAGAGGCCAG CGGCAGCGGC AGGAATGGCC AGGGCTCCGA CCCCGCCGTC 
ACCTACTACC GGCTGGAGGA GGTGGCGAAG CGAAATACCG CGGAGGAGAC GTGGATGGTG 
ATCCACGGGC GAGTCTACGA TATCACCCGC TTCCTCAGCG AGCATCCTGG TGGAGAAGAG 
GTTCTACTGG AACAAGCTGG TGCCGATGCA ACTGAAAGCT TTGAAGATGT CGGCCACTCC 
CCTGATGCCA GGGAGATGCT AAAGCAGTAC TACATTGGTG ATGTCCATCC GAATGACCTT 
AAACCTAAAG ATGGTGACAA GGACCCTTCA AAAAACAATT CATGCCAAAG TTCCTGGGCA 
TATTGGATTG TCCCCATCGT GGGTGCTATT CTTATAGGTT TCCTGTATCG TCACTTCTGG 
GCTGACAGCA AATCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Amidoxime reductase system containing cytochrome b5 type B (CYB5B) and MOSC2 is of importance for lipid synthesis in adipocyte mitochondria.
The Journal of biological chemistry287(9)6307-17(2012 Feb)
Neve EP,Nordling A,Andersson TB,Hellman U,Diczfalusy U,Johansson I,Ingelman-Sundberg M

Accommodating a nonconservative internal mutation by water-mediated hydrogen bonding between ?-sheet strands: a comparison of human and rat type B (mitochondrial) cytochrome b5.
Biochemistry50(24)5544-54(2011 Jun)
Parthasarathy S,Altuve A,Terzyan S,Zhang X,Kuczera K,Rivera M,Benson DR

Biochemical and spectroscopic characterization of the human mitochondrial amidoxime reducing components hmARC-1 and hmARC-2 suggests the existence of a new molybdenum enzyme family in eukaryotes.
The Journal of biological chemistry285(48)37847-59(2010 Nov)
Wahl B,Reichmann D,Niks D,Krompholz N,Havemeyer A,Clement B,Messerschmidt T,Rothkegel M,Biester H,Hille R,Mendel RR,Bittner F

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Influence of point mutations on the flexibility of cytochrome b5: molecular dynamics simulations of holoproteins.
Biopolymers83(3)297-312(2006 Oct)
Cheng Q,Benson DR,Rivera M,Kuczera K