RFA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following RFA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi00845 NM_001178211.1
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Saccharomyces cerevisiae S288C replication factor A subunit protein RFA1 (RFA1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi00845
    Clone ID Related Accession (Same CDS sequence) NM_001178211.1
    Accession Version NM_001178211.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product replication factor A subunit protein RFA1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001133). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGCAGTG TTCAACTTTC GAGGGGCGAT TTTCATAGCA TCTTCACCAA TAAGCAAAGG 
    TACGATAATC CCACCGGTGG CGTTTATCAA GTTTATAACA CCAGGAAATC TGATGGGGCT
    AACAGCAACA GAAAGAATTT GATCATGATT TCCGATGGTA TTTACCATAT GAAGGCTCTG
    TTGAGAAACC AAGCTGCATC CAAGTTCCAG TCAATGGAAC TACAAAGGGG TGATATCATT
    CGCGTGATAA TTGCAGAACC TGCTATTGTC AGGGAAAGAA AGAAATACGT TCTTTTAGTA
    GATGACTTTG AGTTGGTCCA GTCGCGTGCT GATATGGTCA ACCAAACTAG TACTTTTTTG
    GATAACTATT TCTCAGAGCA TCCAAATGAA ACCTTAAAAG ACGAAGATAT AACTGACAGT
    GGTAATGTTG CCAATCAAAC AAACGCCAGC AATGCTGGTG TCCCTGATAT GCTGCATTCA
    AACTCAAACT TGAATGCAAA TGAGAGAAAA TTCGCCAATG AAAACCCTAA TTCGCAAAAA
    ACCAGACCAA TTTTTGCCAT CGAACAACTG TCTCCATACC AAAACGTTTG GACTATCAAA
    GCAAGAGTTT CCTACAAGGG AGAAATTAAA ACGTGGCACA ATCAAAGAGG TGATGGTAAA
    CTATTCAATG TCAACTTCTT GGATACCTCT GGAGAAATCC GAGCCACGGC GTTTAATGAT
    TTTGCTACAA AATTTAACGA AATTTTACAA GAAGGCAAAG TATACTATGT ATCAAAGGCA
    AAACTCCAAC CAGCTAAGCC CCAATTTACT AATCTAACAC ACCCTTATGA ACTGAATTTG
    GATAGAGACA CTGTTATAGA AGAATGTTTC GATGAAAGTA ATGTTCCGAA AACCCATTTC
    AATTTCATCA AACTAGATGC TATTCAGAAC CAGGAAGTAA ATTCCAACGT AGACGTCCTC
    GGTATTATCC AAACTATAAA CCCACATTTT GAGCTAACTT CAAGGGCTGG GAAGAAATTC
    GATCGTCGTG ACATCACAAT TGTTGACGAC TCTGGGTTTT CTATCTCTGT TGGCCTATGG
    AATCAGCAAG CCCTTGATTT CAACCTTCCT GAAGGTTCTG TTGCTGCCAT TAAAGGTGTT
    CGTGTGACGG ATTTTGGTGG CAAATCTTTG TCTATGGGAT TTTCTAGTAC CCTGATTCCG
    AATCCAGAAA TTCCTGAGGC ATATGCCTTA AAGGGTTGGT ATGATTCCAA GGGCCGCAAC
    GCAAACTTCA TCACTTTAAA GCAAGAACCC GGTATGGGTG GTCAATCGGC TGCTAGCTTA
    ACAAAATTCA TTGCTCAGCG TATTACTATT GCTAGAGCTC AAGCTGAAAA TCTAGGAAGA
    AGCGAGAAAG GTGACTTTTT TAGTGTTAAA GCTGCTATAA GTTTCTTAAA AGTTGATAAT
    TTTGCATATC CTGCCTGTTC TAATGAGAAT TGTAATAAGA AAGTTCTGGA ACAGCCTGAT
    GGTACTTGGA GATGTGAGAA GTGCGACACC AATAATGCAA GGCCAAATTG GAGATACATC
    TTGACAATAT CAATTATTGA CGAAACCAAT CAACTATGGC TCACTTTATT TGACGACCAA
    GCTAAACAAT TATTGGGTGT TGATGCTAAT ACATTAATGT CTTTGAAGGA AGAAGACCCC
    AACGAATTCA CAAAAATTAC TCAAAGTATC CAAATGAACG AATATGACTT TAGGATTAGA
    GCGCGTGAGG ATACATACAA TGATCAAAGC AGAATTAGAT ATACCGTTGC TAACCTACAC
    AGCTTGAATT ACAGGGCTGA AGCCGACTAT CTTGCCGATG AGTTATCCAA GGCTTTGTTA
    GCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_009404.1
    CDS1..1866
    Translation

    Target ORF information:

    RefSeq Version NM_001178211.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C replication factor A subunit protein RFA1 (RFA1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001178211.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGCAGTG TTCAACTTTC GAGGGGCGAT TTTCATAGCA TCTTCACCAA TAAGCAAAGG 
    TACGATAATC CCACCGGTGG CGTTTATCAA GTTTATAACA CCAGGAAATC TGATGGGGCT
    AACAGCAACA GAAAGAATTT GATCATGATT TCCGATGGTA TTTACCATAT GAAGGCTCTG
    TTGAGAAACC AAGCTGCATC CAAGTTCCAG TCAATGGAAC TACAAAGGGG TGATATCATT
    CGCGTGATAA TTGCAGAACC TGCTATTGTC AGGGAAAGAA AGAAATACGT TCTTTTAGTA
    GATGACTTTG AGTTGGTCCA GTCGCGTGCT GATATGGTCA ACCAAACTAG TACTTTTTTG
    GATAACTATT TCTCAGAGCA TCCAAATGAA ACCTTAAAAG ACGAAGATAT AACTGACAGT
    GGTAATGTTG CCAATCAAAC AAACGCCAGC AATGCTGGTG TCCCTGATAT GCTGCATTCA
    AACTCAAACT TGAATGCAAA TGAGAGAAAA TTCGCCAATG AAAACCCTAA TTCGCAAAAA
    ACCAGACCAA TTTTTGCCAT CGAACAACTG TCTCCATACC AAAACGTTTG GACTATCAAA
    GCAAGAGTTT CCTACAAGGG AGAAATTAAA ACGTGGCACA ATCAAAGAGG TGATGGTAAA
    CTATTCAATG TCAACTTCTT GGATACCTCT GGAGAAATCC GAGCCACGGC GTTTAATGAT
    TTTGCTACAA AATTTAACGA AATTTTACAA GAAGGCAAAG TATACTATGT ATCAAAGGCA
    AAACTCCAAC CAGCTAAGCC CCAATTTACT AATCTAACAC ACCCTTATGA ACTGAATTTG
    GATAGAGACA CTGTTATAGA AGAATGTTTC GATGAAAGTA ATGTTCCGAA AACCCATTTC
    AATTTCATCA AACTAGATGC TATTCAGAAC CAGGAAGTAA ATTCCAACGT AGACGTCCTC
    GGTATTATCC AAACTATAAA CCCACATTTT GAGCTAACTT CAAGGGCTGG GAAGAAATTC
    GATCGTCGTG ACATCACAAT TGTTGACGAC TCTGGGTTTT CTATCTCTGT TGGCCTATGG
    AATCAGCAAG CCCTTGATTT CAACCTTCCT GAAGGTTCTG TTGCTGCCAT TAAAGGTGTT
    CGTGTGACGG ATTTTGGTGG CAAATCTTTG TCTATGGGAT TTTCTAGTAC CCTGATTCCG
    AATCCAGAAA TTCCTGAGGC ATATGCCTTA AAGGGTTGGT ATGATTCCAA GGGCCGCAAC
    GCAAACTTCA TCACTTTAAA GCAAGAACCC GGTATGGGTG GTCAATCGGC TGCTAGCTTA
    ACAAAATTCA TTGCTCAGCG TATTACTATT GCTAGAGCTC AAGCTGAAAA TCTAGGAAGA
    AGCGAGAAAG GTGACTTTTT TAGTGTTAAA GCTGCTATAA GTTTCTTAAA AGTTGATAAT
    TTTGCATATC CTGCCTGTTC TAATGAGAAT TGTAATAAGA AAGTTCTGGA ACAGCCTGAT
    GGTACTTGGA GATGTGAGAA GTGCGACACC AATAATGCAA GGCCAAATTG GAGATACATC
    TTGACAATAT CAATTATTGA CGAAACCAAT CAACTATGGC TCACTTTATT TGACGACCAA
    GCTAAACAAT TATTGGGTGT TGATGCTAAT ACATTAATGT CTTTGAAGGA AGAAGACCCC
    AACGAATTCA CAAAAATTAC TCAAAGTATC CAAATGAACG AATATGACTT TAGGATTAGA
    GCGCGTGAGG ATACATACAA TGATCAAAGC AGAATTAGAT ATACCGTTGC TAACCTACAC
    AGCTTGAATT ACAGGGCTGA AGCCGACTAT CTTGCCGATG AGTTATCCAA GGCTTTGTTA
    GCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
    Proceedings of the National Academy of Sciences of the United States of America92(9)3809-13(1995 Apr)
    Bussey H,Kaback DB,Zhong W,Vo DT,Clark MW,Fortin N,Hall J,Ouellette BF,Keng T,Barton AB


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