act1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following act1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the act1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc101220 NM_001021513.2
Latest version!
Schizosaccharomyces pombe actin Act1 (act1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc101220
    Clone ID Related Accession (Same CDS sequence) NM_001021513.2
    Accession Version NM_001021513.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product actin Act1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021513.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGAAGAAG AAATCGCAGC GTTGGTTATT GATAATGGCT CTGGTATGTG CAAAGCCGGT 
    TTCGCTGGAG ATGATGCCCC TAGAGCTGTA TTCCCCTCGA TTGTCGGTAG ACCCCGTCAC
    CATGGTATTA TGGTAGGTAT GGGACAAAAG GATTCCTACG TTGGTGATGA AGCTCAAAGC
    AAGCGTGGTA TTTTGACCTT GAAGTACCCC ATTGAGCACG GTATTGTCAA CAACTGGGAT
    GATATGGAAA AGATTTGGCA TCACACTTTC TACAACGAGC TTCGTGTTGC TCCTGAGGAG
    CACCCTTGCT TGTTGACTGA GGCTCCTTTG AACCCCAAAT CCAACCGTGA GAAGATGACT
    CAAATTATTT TCGAAACCTT TAATGCACCT GCCTTTTATG TTGCTATTCA AGCTGTTTTG
    TCTTTGTATG CCTCTGGTCG TACCACTGGT ATCGTCTTGG ACTCTGGTGA TGGTGTTACC
    CACACTGTTC CCATTTATGA GGGTTATGCT CTTCCTCATG CTATCATGCG TCTTGATCTC
    GCCGGTCGTG ACTTGACTGA CTACCTTATG AAGATTCTCA TGGAGCGTGG TTATACTTTC
    TCTACTACCG CCGAACGTGA AATTGTTCGT GACATCAAGG AGAAACTTTG CTACGTCGCT
    TTGGACTTTG AGCAAGAACT TCAAACTGCT GCTCAATCTT CCTCCCTTGA GAAATCTTAT
    GAGCTTCCTG ATGGTCAAGT TATCACTATC GGAAACGAGC GTTTCCGTGC CCCTGAAGCT
    CTTTTCCAAC CCTCAGCTTT GGGTCTTGAA AACGCCGGTA TTCATGAGGC TACTTACAAC
    TCTATCATGA AATGTGATGT TGATATTCGT AAAGATTTGT ACGGTAACGT TGTTATGTCT
    GGTGGTACCA CTATGTATCC CGGTATTGCC GATCGTATGC AAAAGGAGAT TCAAGCCCTT
    GCTCCTAGCT CCATGAAGGT CAAGATTGTT GCTCCTCCTG AGCGTAAATA CTCTGTCTGG
    ATTGGTGGAT CCATTCTTGC TTCTCTTTCT ACTTTCCAAC AGATGTGGAT CTCCAAGCAA
    GAGTATGATG AGAGCGGACC TGGTATCGTT TACCGTAAGT GCTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595618.1
    CDS42..1169
    Translation

    Target ORF information:

    RefSeq Version NM_001021513.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe actin Act1 (act1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001021513.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGAAGAAG AAATCGCAGC GTTGGTTATT GATAATGGCT CTGGTATGTG CAAAGCCGGT 
    TTCGCTGGAG ATGATGCCCC TAGAGCTGTA TTCCCCTCGA TTGTCGGTAG ACCCCGTCAC
    CATGGTATTA TGGTAGGTAT GGGACAAAAG GATTCCTACG TTGGTGATGA AGCTCAAAGC
    AAGCGTGGTA TTTTGACCTT GAAGTACCCC ATTGAGCACG GTATTGTCAA CAACTGGGAT
    GATATGGAAA AGATTTGGCA TCACACTTTC TACAACGAGC TTCGTGTTGC TCCTGAGGAG
    CACCCTTGCT TGTTGACTGA GGCTCCTTTG AACCCCAAAT CCAACCGTGA GAAGATGACT
    CAAATTATTT TCGAAACCTT TAATGCACCT GCCTTTTATG TTGCTATTCA AGCTGTTTTG
    TCTTTGTATG CCTCTGGTCG TACCACTGGT ATCGTCTTGG ACTCTGGTGA TGGTGTTACC
    CACACTGTTC CCATTTATGA GGGTTATGCT CTTCCTCATG CTATCATGCG TCTTGATCTC
    GCCGGTCGTG ACTTGACTGA CTACCTTATG AAGATTCTCA TGGAGCGTGG TTATACTTTC
    TCTACTACCG CCGAACGTGA AATTGTTCGT GACATCAAGG AGAAACTTTG CTACGTCGCT
    TTGGACTTTG AGCAAGAACT TCAAACTGCT GCTCAATCTT CCTCCCTTGA GAAATCTTAT
    GAGCTTCCTG ATGGTCAAGT TATCACTATC GGAAACGAGC GTTTCCGTGC CCCTGAAGCT
    CTTTTCCAAC CCTCAGCTTT GGGTCTTGAA AACGCCGGTA TTCATGAGGC TACTTACAAC
    TCTATCATGA AATGTGATGT TGATATTCGT AAAGATTTGT ACGGTAACGT TGTTATGTCT
    GGTGGTACCA CTATGTATCC CGGTATTGCC GATCGTATGC AAAAGGAGAT TCAAGCCCTT
    GCTCCTAGCT CCATGAAGGT CAAGATTGTT GCTCCTCCTG AGCGTAAATA CTCTGTCTGG
    ATTGGTGGAT CCATTCTTGC TTCTCTTTCT ACTTTCCAAC AGATGTGGAT CTCCAAGCAA
    GAGTATGATG AGAGCGGACC TGGTATCGTT TACCGTAAGT GCTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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