CARTPT cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	CARTPT
Entrez Gene ID	397252
Full Name	CART prepropeptide
Synonyms	CART
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001099925.1	NP_001093395.1	cocaine- and amphetamine-regulated transcript protein precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Distribution and immunohistochemical characteristics of cocaine- and amphetamineregulated transcript-positive nerve elements in the pelvic ganglia of the female pig.
Zacharko-Siembida A, Matysek M, Szalak R, Radli?ska A, Obsza?ska K, Arciszewski MB
Polish journal of veterinary sciences20(1)173-183(2017 Mar)

T2 Toxin-Induced Changes in Cocaine- and Amphetamine-Regulated Transcript (CART)-Like Immunoreactivity in the Enteric Nervous System Within Selected Fragments of the Porcine Digestive Tract.
Makowska K, Gonkowski S, Zielonka L, Dabrowski M, Calka J
Neurotoxicity research31(1)136-147(2017 Jan)

Expression of Cocaine and Amphetamine Regulated Transcript (CART) in the Porcine Intramural Neurons of Stomach in the Course of Experimentally Induced Diabetes Mellitus.
Bulc M, Gonkowski S, Ca?ka J
Journal of molecular neuroscience : MN57(3)376-85(2015 Nov)

The biochemical utility of chromogranin A, chromogranin B and cocaine- and amphetamine-regulated transcript for neuroendocrine neoplasia.
Bech PR, Martin NM, Ramachandran R, Bloom SR
Annals of clinical biochemistry51(Pt 1)8-21(2014 Jan)

Co-localisation of cocaine- and amphetamine-regulated transcript peptide and vasoactive intestinal polypeptide in the myenteric plexus of the porcine transverse colon.
Palus K, Rytel L
Folia morphologica72(4)328-32(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study showed that CART present in the pelvic plexus (PP) may influence the activity of pelvic ganglionic neurons/SIF cells, that the PP should be considered as a potential source of CART-like supply to pelvic viscera, and that functional interactions between CART and substance P or PACAP are possible at the periphery.
Title: Distribution and immunohistochemical characteristics of cocaine- and amphetamineregulated transcript-positive nerve elements in the pelvic ganglia of the female pig.

Results show that even low doses of T-2 toxin may change the expression of CART in the nervous system, and confirms that CART seems to be an important neuronal factor within the enteric nervous system, which takes part in regulation of intestinal functions both in physiological conditions and during pathological states.
Title: T2 Toxin-Induced Changes in Cocaine- and Amphetamine-Regulated Transcript (CART)-Like Immunoreactivity in the Enteric Nervous System Within Selected Fragments of the Porcine Digestive Tract.

CART-LI perikarya decreased in myenteric plexus of the gastric antrum, corpus, and pylorus in experimental diabetes. Acute hyperglycemia reduced CART expression in enteric perikarya and decreased CART-LI fiber density in gastric antrum circular muscle.
Title: Expression of Cocaine and Amphetamine Regulated Transcript (CART) in the Porcine Intramural Neurons of Stomach in the Course of Experimentally Induced Diabetes Mellitus.

CgA and CgB have been used as general NEN biomarkers for many years, while CART has only recently been identified
Title: The biochemical utility of chromogranin A, chromogranin B and cocaine- and amphetamine-regulated transcript for neuroendocrine neoplasia.

The present study reports for the first time on the co-localisation of CART and VIP in myenteric neurons of the porcine transverse colon.
Title: Co-localisation of cocaine- and amphetamine-regulated transcript peptide and vasoactive intestinal polypeptide in the myenteric plexus of the porcine transverse colon.

The following CARTPT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CARTPT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00593	NM_001099925.1 Latest version!	Sus scrofa CART prepropeptide (CARTPT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CARTPT ( NM_001099925.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001093395.1 Sus scrofa CART prepropeptide (CARTPT), mRNA.

Price & Availability↑

CloneID	OSe00593
Clone ID Related Accession (Same CDS sequence)	NM_001099925.1
Accession Version	NM_001099925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Sus scrofa(pig)
Product	cocaine- and amphetamine-regulated transcript protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF581838.1. ##Evidence-Data-START## Transcript exon combination :: CF363900.1, CF361864.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1487420, SAMEA1876824 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAGCC CCCGCCTGCG GCTGCTGCCC CTGCTAGGTG CCGCCCTGCT GCTGCTGCTA 
CCTCTGCTGG GCGCCCTTGC CCAGGAGGAT GCCGAGCTCC AGCCGCGAGC CCTGGACATC 
TACTCTGCCG TGGAGGATGC CTCCCATGAG AAGGAGCTGA TTGAAGCGCT GCAGGAAGTC 
CTGAAGAAGC TCAAGAGTAA ACGTATCCCG ATTTATGAGA AGAAGTATGG CCAAGTCCCT 
ATGTGTGACG CAGGAGAGCA GTGCGCCGTG CGAAAAGGAG CTAGAATCGG GAAGCTGTGT 
GACTGTCCCC GAGGAACCTC CTGCAATTCC TTCCTCCTGA AGTGCTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093395.1
CDS	11..361
Translation	MESPRLRLLPLLGAALLLLLPLLGALAQEDAELQPRALDIYSAVEDASHEKELIEALQEVLKKLKSKRIPIYEKKYGQVPMCDAGEQCAVRKGARIGKLCDCPRGTSCNSFLLKCL

Target ORF information:

RefSeq Version	NM_001099925.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa CART prepropeptide (CARTPT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099925.1

ORF Insert Sequence:

ATGGAGAGCC CCCGCCTGCG GCTGCTGCCC CTGCTAGGTG CCGCCCTGCT GCTGCTGCTA 
CCTCTGCTGG GCGCCCTTGC CCAGGAGGAT GCCGAGCTCC AGCCGCGAGC CCTGGACATC 
TACTCTGCCG TGGAGGATGC CTCCCATGAG AAGGAGCTGA TTGAAGCGCT GCAGGAAGTC 
CTGAAGAAGC TCAAGAGTAA ACGTATCCCG ATTTATGAGA AGAAGTATGG CCAAGTCCCT 
ATGTGTGACG CAGGAGAGCA GTGCGCCGTG CGAAAAGGAG CTAGAATCGG GAAGCTGTGT 
GACTGTCCCC GAGGAACCTC CTGCAATTCC TTCCTCCTGA AGTGCTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distribution and immunohistochemical characteristics of cocaine- and amphetamineregulated transcript-positive nerve elements in the pelvic ganglia of the female pig.
Polish journal of veterinary sciences20(1)173-183(2017 Mar)
Zacharko-Siembida A,Matysek M,Szalak R,Radli?ska A,Obsza?ska K,Arciszewski MB

T2 Toxin-Induced Changes in Cocaine- and Amphetamine-Regulated Transcript (CART)-Like Immunoreactivity in the Enteric Nervous System Within Selected Fragments of the Porcine Digestive Tract.
Neurotoxicity research31(1)136-147(2017 Jan)
Makowska K,Gonkowski S,Zielonka L,Dabrowski M,Calka J

Expression of Cocaine and Amphetamine Regulated Transcript (CART) in the Porcine Intramural Neurons of Stomach in the Course of Experimentally Induced Diabetes Mellitus.
Journal of molecular neuroscience : MN57(3)376-85(2015 Nov)
Bulc M,Gonkowski S,Ca?ka J

The biochemical utility of chromogranin A, chromogranin B and cocaine- and amphetamine-regulated transcript for neuroendocrine neoplasia.
Annals of clinical biochemistry51(Pt 1)8-21(2014 Jan)
Bech PR,Martin NM,Ramachandran R,Bloom SR

Co-localisation of cocaine- and amphetamine-regulated transcript peptide and vasoactive intestinal polypeptide in the myenteric plexus of the porcine transverse colon.
Folia morphologica72(4)328-32(2013 Nov)
Palus K,Rytel L