LOC660129 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC660129
Entrez Gene ID	660129
Full Name	membrane-bound alkaline phosphatase
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_966389.4	XP_971482.1	membrane-bound alkaline phosphatase

Pathways from BioSystems
Gene Ontology

The following LOC660129 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC660129 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg11960	XM_966389.4 Latest version!	Tribolium castaneum membrane-bound alkaline phosphatase (LOC660129), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC660129 ( XM_966389.4 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_971482.1 Tribolium castaneum membrane-bound alkaline phosphatase (LOC660129), mRNA.

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CloneID	OTg11960
Clone ID Related Accession (Same CDS sequence)	XM_966389.4
Accession Version	XM_966389.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	membrane-bound alkaline phosphatase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450185.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_966389.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTCA TCACTCTAAT CCTTTTCCTA GCCTTTGCCC AAACCTGGGC CTCCTCTATG 
CACCCCCGTC TCCCCCTCCC CACCAAACAC TCCCCCAACC CCGACGAACG AACCTCCAGC 
TTCTGGATCC AAAACGCCCA AAAAACCGTC AGCGAAAAAA TCAAACGCGA ACTCAACCAA 
AACGTAGCCA AAAACGTGAT TCTTTTCCTG GGGGACGGCA TGTCAATACC AACCGTGTCG 
GCAGCCAGGG TGTACCAATC AGGGGAGGAA ACCGAATTAG CTTTCGACAA ATTCCCCTAC 
ACTGGACTAG CCAAGACGTA CGCGGTCGAC AGACAAGTGG CCGATTCGGC CAACACGGCC 
ACGGCCTACT TGACCGGAAT TAAGGCAAAC TACGGAACAA TCGGAGTGGG ACCTGAAGTG 
AACCGAAGCG ACTGTCAAGG AATGACAGAT GAAACAAACC ACGCCCTGTC CATAGCCTAC 
TACTCCCAAT TAAAAAACAA ACGGACAGGT CTAGTGACCA CGGCTCGCGT CACCCATGCC 
TCGCCTGCTG GGGTTTATGC CCACACGAGT GACAGAAACT GGGAAAGTGA CACTGACGTC 
ATTAATGACG GTCAGAACCC GCAAATATGT TCCGACATTG CCACTCAATT GTTATTTAGT 
GAAACTGGTA AAAAGTTGAA CGTTGTTATG GGCGGGGGCA GAAAAAAGCT CCTCCCTAAA 
GAAATGACAG ATGAGGAAGG CCACAAAGGT GAAAGGTCAG ACGGCGCCAA CTTGATCCAA 
AAATGGCAAG AGCAGCGCAT TAACGTCAAG TATGCCTGGA ACCGCAGCCA ACTGATCGAC 
TCTGACGTGA CCTTTGACAA CGTTTTGGGC CTTTTTGCCC ACGACCACAT CAGTTATAAC 
CTTGAACGTG ACAATATCAG CGACCCCTCA TTGGCCGAAA TGACCGCGGC GGCCATTAAA 
ACCCTAAATC GGGGTTCCGA AGGCTACTTT CTCTTCGTAG AAGGCGCCCG TATCGACATG 
GCACACCACG AAACCGCATC GCGGATAGCC TTGGACGAAA CAATCGAATT CTCCAAGGCC 
ATCCAAGTCG CCGTTGACTT GACCGACGAA TCCGATACGC TGATCGTCGT GACGTCAGAC 
CACGCCCACA CAATGAGTTA CAGCGGATAT GCCGAACGCG GCCATGACAT ACTCGAATTT 
GGCGGCGATG GTAGTGATGG TAAAGTCTAC ACGACCTTGA ATTACGCCAA TGGGCCTGGG 
TATAAAAAGC CAAATTCGGA TGGGTCACGT CATGATATCG CGGGAGATAA TTATGGTGAT 
AAGCATTACG CATTTCCGGC TGTTGCGCCC TTATATGCCG AAACACATGG CGGTGATGAC 
GTTGCAGTTT TTGCAAGAGG ACCATGGGCC CACCTTTTTA GCGGGGTCTT GGAACAAAAT 
GTTATCCCGC ATTTGATGGC CTATGCCTCT TGTGTGGGCG AAGGATTGAC TGCTTGTGAT 
CAAATCAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_971482.1
CDS	32..1543
Translation	MSFITLILFLAFAQTWASSMHPRLPLPTKHSPNPDERTSSFWIQNAQKTVSEKIKRELNQNVAKNVILFLGDGMSIPTVSAARVYQSGEETELAFDKFPYTGLAKTYAVDRQVADSANTATAYLTGIKANYGTIGVGPEVNRSDCQGMTDETNHALSIAYYSQLKNKRTGLVTTARVTHASPAGVYAHTSDRNWESDTDVINDGQNPQICSDIATQLLFSETGKKLNVVMGGGRKKLLPKEMTDEEGHKGERSDGANLIQKWQEQRINVKYAWNRSQLIDSDVTFDNVLGLFAHDHISYNLERDNISDPSLAEMTAAAIKTLNRGSEGYFLFVEGARIDMAHHETASRIALDETIEFSKAIQVAVDLTDESDTLIVVTSDHAHTMSYSGYAERGHDILEFGGDGSDGKVYTTLNYANGPGYKKPNSDGSRHDIAGDNYGDKHYAFPAVAPLYAETHGGDDVAVFARGPWAHLFSGVLEQNVIPHLMAYASCVGEGLTACDQIN

Target ORF information:

RefSeq Version	XM_966389.4
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum membrane-bound alkaline phosphatase (LOC660129), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_966389.4

ORF Insert Sequence:

ATGTCCTTCA TCACTCTAAT CCTTTTCCTA GCCTTTGCCC AAACCTGGGC CTCCTCTATG 
CACCCCCGTC TCCCCCTCCC CACCAAACAC TCCCCCAACC CCGACGAACG AACCTCCAGC 
TTCTGGATCC AAAACGCCCA AAAAACCGTC AGCGAAAAAA TCAAACGCGA ACTCAACCAA 
AACGTAGCCA AAAACGTGAT TCTTTTCCTG GGGGACGGCA TGTCAATACC AACCGTGTCG 
GCAGCCAGGG TGTACCAATC AGGGGAGGAA ACCGAATTAG CTTTCGACAA ATTCCCCTAC 
ACTGGACTAG CCAAGACGTA CGCGGTCGAC AGACAAGTGG CCGATTCGGC CAACACGGCC 
ACGGCCTACT TGACCGGAAT TAAGGCAAAC TACGGAACAA TCGGAGTGGG ACCTGAAGTG 
AACCGAAGCG ACTGTCAAGG AATGACAGAT GAAACAAACC ACGCCCTGTC CATAGCCTAC 
TACTCCCAAT TAAAAAACAA ACGGACAGGT CTAGTGACCA CGGCTCGCGT CACCCATGCC 
TCGCCTGCTG GGGTTTATGC CCACACGAGT GACAGAAACT GGGAAAGTGA CACTGACGTC 
ATTAATGACG GTCAGAACCC GCAAATATGT TCCGACATTG CCACTCAATT GTTATTTAGT 
GAAACTGGTA AAAAGTTGAA CGTTGTTATG GGCGGGGGCA GAAAAAAGCT CCTCCCTAAA 
GAAATGACAG ATGAGGAAGG CCACAAAGGT GAAAGGTCAG ACGGCGCCAA CTTGATCCAA 
AAATGGCAAG AGCAGCGCAT TAACGTCAAG TATGCCTGGA ACCGCAGCCA ACTGATCGAC 
TCTGACGTGA CCTTTGACAA CGTTTTGGGC CTTTTTGCCC ACGACCACAT CAGTTATAAC 
CTTGAACGTG ACAATATCAG CGACCCCTCA TTGGCCGAAA TGACCGCGGC GGCCATTAAA 
ACCCTAAATC GGGGTTCCGA AGGCTACTTT CTCTTCGTAG AAGGCGCCCG TATCGACATG 
GCACACCACG AAACCGCATC GCGGATAGCC TTGGACGAAA CAATCGAATT CTCCAAGGCC 
ATCCAAGTCG CCGTTGACTT GACCGACGAA TCCGATACGC TGATCGTCGT GACGTCAGAC 
CACGCCCACA CAATGAGTTA CAGCGGATAT GCCGAACGCG GCCATGACAT ACTCGAATTT 
GGCGGCGATG GTAGTGATGG TAAAGTCTAC ACGACCTTGA ATTACGCCAA TGGGCCTGGG 
TATAAAAAGC CAAATTCGGA TGGGTCACGT CATGATATCG CGGGAGATAA TTATGGTGAT 
AAGCATTACG CATTTCCGGC TGTTGCGCCC TTATATGCCG AAACACATGG CGGTGATGAC 
GTTGCAGTTT TTGCAAGAGG ACCATGGGCC CACCTTTTTA GCGGGGTCTT GGAACAAAAT 
GTTATCCCGC ATTTGATGGC CTATGCCTCT TGTGTGGGCG AAGGATTGAC TGCTTGTGAT 
CAAATCAATT AA