LOC660189 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC660189
Entrez Gene ID	660189
Full Name	ER degradation-enhancing alpha-mannosidase-like protein 3
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_966443.3	XP_971536.1	ER degradation-enhancing alpha-mannosidase-like protein 3

Pathways from BioSystems
Gene Ontology

The following LOC660189 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC660189 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg11201	XM_966443.3 Latest version!	Tribolium castaneum ER degradation-enhancing alpha-mannosidase-like protein 3 (LOC660189), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC660189 ( XM_966443.3 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_971536.1 Tribolium castaneum ER degradation-enhancing alpha-mannosidase-like protein 3 (LOC660189), mRNA.

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CloneID	OTg11201
Clone ID Related Accession (Same CDS sequence)	XM_966443.3
Accession Version	XM_966443.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450181.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_966443.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTT TGGAGGTGCT AGTGCGGGTG TTTTTCGTGA GTGCTTTGGT GGGCTTCTGC 
TGCCCGGAGG AGGCCCCCAT GACCAGAGCG GAGCGCCACC GGCTGAGGGA GGAGACCCGG 
GACATGTTCT ACCACGCGTA CTGGGCCTAC ATGGAGAACG CGTATCCTGC CGACGAGCTG 
ATGCCCCTGA GCTGCAAGGG CAGGTTCAGG GGGCTCACCC CCAACCGGGG GGACATCGAC 
GACTGCCTGG GCAATTTCTC ACTTACACTA ATTGATACTC TGGACTCTTT AGTGGTGTTA 
GGTGATTTAG AAGAATTCGA ACATGCCGTC AAGCTTGTAA TAAAAGACGT TTCGTTTGAT 
AACGATGTCG TCGTTTCAGT TTTCGAAACA AATATTCGAG TTTTAGGTGG ACTTTTATCA 
GCCCACATTT TGGCCGATTA TTTGCAACAA AGGGACGGGA TTATGCCCTG GTATAAGGGC 
GAACTTCTCA ACATGGCCAA AGATGTGGGC TATCGGCTTT TACCGGCATT TAACACCACG 
ACAGGAATAC CACATTCGAG GGTGAATATG AAATATGGGT TAAAGAGCGA TAGATTGGAG 
TCGGCGAGGG AGACGTGTAC AGCATGTGCT GGGTCTATGA TTTTAGAGAT GGCAGCACTG 
TCTCGACTGA CTGGAGAACC GATTTTCGAG GAGAAGGCTC ATAAAGCGAT GGATGAGTTG 
TGGAAGATGA GACATCGAAG TTCGGATTTA ATGGGAACTG TTTTGAATGT TCATTCGGGA 
GATTGGGTTC GAAGGGATTC GGGAGTGGGA GCTGGGATTG ATTCCTATTA CGAGTACTGT 
CTCAAGGCGT ATATTTTGCT GGGGGATAAC AAGTACTTGC ATAGGTTTAA TCGGCACTAT 
AATGCAGTCA TGAAGTATAT ATCGCAAGGT CCGATGTTGC TTGACGTGCA CATGCACCGC 
CCCCACACCA ACTCCCGCAA CTACATGGAC GCCCTCCTCG CCTTCTGGCC CGGCCTCCAG 
GTGCTCAAAG GCGACCTCAA ACCAGCCGTC GAAACCCACG AGATGCTCTA CCAGGTCATG 
CAGCGCCACA CCTTCATCCC CGAAGCCTTC ACCACCGACT TCCAGGTCCA CTGGGGCAAC 
CACCCCCTCC GACCCGAATT TCTCGAATCC ACTTACTTCC TCTACGGTGC CACAAACGAC 
CCCTACTATC TCGAAGTGGG CAGGACCGTA CTCAACAGTT TACAAAAGTA CGCCCGAGTG 
CCTTGCGGCT ATGCCGCCGT TAACGACGTC CGGACACGCA AACAGGAGGA TCGGATGGAT 
TCGTTTGTGT TGTCCGAAAC GTTGAAATAC TTGTATTTGT TGTTTGCCGA TAAGGAGGAT 
TTGATTTTGA ATTTGGATGA GTTTATTTTT ACGACGGAAG GTCATTTGTT GCCGTTGAGT 
TTGGCGGGGT ACAATGGTAA TAAGACTGTG AGCGAATTGG ATGTGGAGGA GGGGGAGTTT 
GCGAGGATTT GTCCCAATAC GTTACAGTTG TTCCCCGAAA CGGTGAGGAA GCCGTTACAG 
AATATGGTCG ATGGGATGTG TCCCAAGAGG AATACGAAGA GGAGGCTGAC AGCTGTCGAG 
TTCTCGGCCT CGAATATCAA TCATTTGAAG ATGATTAAGG ATATGGGGAT CAACATTATT 
GCGTTGAGTG ATGGTCGGGT GCAGCTGCTA CACACGTTTT CATCGGCCCG TTCGAGCGCC 
GACGCCGAAG AGGGCCTCCT CTTCATGCAA GAAATGATGG AACTCTCGAA ACTCCAAACC 
CAACAACTGG ACGCCACCCC CCAATCAGTC ACCTTTCCCA TCAAAGACCA AACCCTCACA 
GTCCAGGCCT GCCCCTCGCA GTTCGGCAAG AACCTCCAAG GCGACCAGAA AATCACCGCT 
CGCACCATCA TCATCCACCC GTTCCGCGCC TGCTCCGACC TCGTCCAGAC CGAGGCCATC 
AAGGGGAAAA TCGCCATCAT GGAGCGCGGC GACTGCATGT TCGTCGACAA GGCGCGCAAA 
GTGCAAAAGC ACGGTGCCGT GGCCGCCATC ATCATCGACA ACACACCAGG AAGCAGCGCC 
GCCTCCTCGC CGATGTTCTC CATGTCGGGC GACGGCACCG ACGACGTCGC CATCCCCACG 
GTGTTCCTGT TCGCCCAGGA CGCCTCCAAG CTGCTGTTCG CGCTGAGCAG GGACCCCAGC 
ATCGAAGTCA CCATAGGCGA GTATAAAAAC GAAGGTGAGG CGTGGGCGCA CAGCGAGGAG 
GAAAGTATGT TTCATAAATT GAAAGTCTCC GTCCAGGAGT TTTTGAATAA ACACACCGGG 
ATTGCGTTCA GTAAGTCGGT CAAAGTTGGC GCGTTTAGGG CGAGTTTAGG GTCTGATAAA 
ATACGGATAA CGTACGAGGA GGGCGAGGAG GGGGGGAAGG TCGCCGGGGT GGGAGATACA 
GTGACCACGC CCCAGTGGGT GCAAGTCCGT AGGGATTTGC TCAAGTCGAT TATGACGTCA 
GAGGGGAAGG AATTGTACGT CCCTTTGAAT ATTTTGCGGA TTTATTATCA AACATTAAGT 
GATGATCCGA TGGAAGATAA GAGAGTGCAA GATACGGTCA AGCAGGCCGA GTGGTTGCTC 
AATCAACTGT CCATTGAATT GGCCAATAAG GACGCGTTGG TTAAACCGGA GGATGGGGTT 
AAGGTGTTGT CGGTATTAAC CGACTTGGGT CAGTTGGCTG GGAGCGATCT CGAGGATAAG 
GAAACACTAA AGGCGCTGAA TTCGATTCTG GAAAGTGTGG CCAAGGATGT TAAGTTCAAG 
AAGGAACATT TGGGTGCGGT TATGAAGAAG TTGCAAGAGG CGGATGATGA TTTGGTGGTC 
AGTGATGTTA AGCCCAAAGA GACGGACAGG GATTCTAGTG AAATTAATAG TAGTGATAGT 
GAACATATTC AGACGAAAAT CAGTCACGCC GTTGATGAGT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_971536.1
CDS	494..3478
Translation	MSALEVLVRVFFVSALVGFCCPEEAPMTRAERHRLREETRDMFYHAYWAYMENAYPADELMPLSCKGRFRGLTPNRGDIDDCLGNFSLTLIDTLDSLVVLGDLEEFEHAVKLVIKDVSFDNDVVVSVFETNIRVLGGLLSAHILADYLQQRDGIMPWYKGELLNMAKDVGYRLLPAFNTTTGIPHSRVNMKYGLKSDRLESARETCTACAGSMILEMAALSRLTGEPIFEEKAHKAMDELWKMRHRSSDLMGTVLNVHSGDWVRRDSGVGAGIDSYYEYCLKAYILLGDNKYLHRFNRHYNAVMKYISQGPMLLDVHMHRPHTNSRNYMDALLAFWPGLQVLKGDLKPAVETHEMLYQVMQRHTFIPEAFTTDFQVHWGNHPLRPEFLESTYFLYGATNDPYYLEVGRTVLNSLQKYARVPCGYAAVNDVRTRKQEDRMDSFVLSETLKYLYLLFADKEDLILNLDEFIFTTEGHLLPLSLAGYNGNKTVSELDVEEGEFARICPNTLQLFPETVRKPLQNMVDGMCPKRNTKRRLTAVEFSASNINHLKMIKDMGINIIALSDGRVQLLHTFSSARSSADAEEGLLFMQEMMELSKLQTQQLDATPQSVTFPIKDQTLTVQACPSQFGKNLQGDQKITARTIIIHPFRACSDLVQTEAIKGKIAIMERGDCMFVDKARKVQKHGAVAAIIIDNTPGSSAASSPMFSMSGDGTDDVAIPTVFLFAQDASKLLFALSRDPSIEVTIGEYKNEGEAWAHSEEESMFHKLKVSVQEFLNKHTGIAFSKSVKVGAFRASLGSDKIRITYEEGEEGGKVAGVGDTVTTPQWVQVRRDLLKSIMTSEGKELYVPLNILRIYYQTLSDDPMEDKRVQDTVKQAEWLLNQLSIELANKDALVKPEDGVKVLSVLTDLGQLAGSDLEDKETLKALNSILESVAKDVKFKKEHLGAVMKKLQEADDDLVVSDVKPKETDRDSSEINSSDSEHIQTKISHAVDEL

Target ORF information:

RefSeq Version	XM_966443.3
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum ER degradation-enhancing alpha-mannosidase-like protein 3 (LOC660189), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_966443.3

ORF Insert Sequence:

ATGAGTGCTT TGGAGGTGCT AGTGCGGGTG TTTTTCGTGA GTGCTTTGGT GGGCTTCTGC 
TGCCCGGAGG AGGCCCCCAT GACCAGAGCG GAGCGCCACC GGCTGAGGGA GGAGACCCGG 
GACATGTTCT ACCACGCGTA CTGGGCCTAC ATGGAGAACG CGTATCCTGC CGACGAGCTG 
ATGCCCCTGA GCTGCAAGGG CAGGTTCAGG GGGCTCACCC CCAACCGGGG GGACATCGAC 
GACTGCCTGG GCAATTTCTC ACTTACACTA ATTGATACTC TGGACTCTTT AGTGGTGTTA 
GGTGATTTAG AAGAATTCGA ACATGCCGTC AAGCTTGTAA TAAAAGACGT TTCGTTTGAT 
AACGATGTCG TCGTTTCAGT TTTCGAAACA AATATTCGAG TTTTAGGTGG ACTTTTATCA 
GCCCACATTT TGGCCGATTA TTTGCAACAA AGGGACGGGA TTATGCCCTG GTATAAGGGC 
GAACTTCTCA ACATGGCCAA AGATGTGGGC TATCGGCTTT TACCGGCATT TAACACCACG 
ACAGGAATAC CACATTCGAG GGTGAATATG AAATATGGGT TAAAGAGCGA TAGATTGGAG 
TCGGCGAGGG AGACGTGTAC AGCATGTGCT GGGTCTATGA TTTTAGAGAT GGCAGCACTG 
TCTCGACTGA CTGGAGAACC GATTTTCGAG GAGAAGGCTC ATAAAGCGAT GGATGAGTTG 
TGGAAGATGA GACATCGAAG TTCGGATTTA ATGGGAACTG TTTTGAATGT TCATTCGGGA 
GATTGGGTTC GAAGGGATTC GGGAGTGGGA GCTGGGATTG ATTCCTATTA CGAGTACTGT 
CTCAAGGCGT ATATTTTGCT GGGGGATAAC AAGTACTTGC ATAGGTTTAA TCGGCACTAT 
AATGCAGTCA TGAAGTATAT ATCGCAAGGT CCGATGTTGC TTGACGTGCA CATGCACCGC 
CCCCACACCA ACTCCCGCAA CTACATGGAC GCCCTCCTCG CCTTCTGGCC CGGCCTCCAG 
GTGCTCAAAG GCGACCTCAA ACCAGCCGTC GAAACCCACG AGATGCTCTA CCAGGTCATG 
CAGCGCCACA CCTTCATCCC CGAAGCCTTC ACCACCGACT TCCAGGTCCA CTGGGGCAAC 
CACCCCCTCC GACCCGAATT TCTCGAATCC ACTTACTTCC TCTACGGTGC CACAAACGAC 
CCCTACTATC TCGAAGTGGG CAGGACCGTA CTCAACAGTT TACAAAAGTA CGCCCGAGTG 
CCTTGCGGCT ATGCCGCCGT TAACGACGTC CGGACACGCA AACAGGAGGA TCGGATGGAT 
TCGTTTGTGT TGTCCGAAAC GTTGAAATAC TTGTATTTGT TGTTTGCCGA TAAGGAGGAT 
TTGATTTTGA ATTTGGATGA GTTTATTTTT ACGACGGAAG GTCATTTGTT GCCGTTGAGT 
TTGGCGGGGT ACAATGGTAA TAAGACTGTG AGCGAATTGG ATGTGGAGGA GGGGGAGTTT 
GCGAGGATTT GTCCCAATAC GTTACAGTTG TTCCCCGAAA CGGTGAGGAA GCCGTTACAG 
AATATGGTCG ATGGGATGTG TCCCAAGAGG AATACGAAGA GGAGGCTGAC AGCTGTCGAG 
TTCTCGGCCT CGAATATCAA TCATTTGAAG ATGATTAAGG ATATGGGGAT CAACATTATT 
GCGTTGAGTG ATGGTCGGGT GCAGCTGCTA CACACGTTTT CATCGGCCCG TTCGAGCGCC 
GACGCCGAAG AGGGCCTCCT CTTCATGCAA GAAATGATGG AACTCTCGAA ACTCCAAACC 
CAACAACTGG ACGCCACCCC CCAATCAGTC ACCTTTCCCA TCAAAGACCA AACCCTCACA 
GTCCAGGCCT GCCCCTCGCA GTTCGGCAAG AACCTCCAAG GCGACCAGAA AATCACCGCT 
CGCACCATCA TCATCCACCC GTTCCGCGCC TGCTCCGACC TCGTCCAGAC CGAGGCCATC 
AAGGGGAAAA TCGCCATCAT GGAGCGCGGC GACTGCATGT TCGTCGACAA GGCGCGCAAA 
GTGCAAAAGC ACGGTGCCGT GGCCGCCATC ATCATCGACA ACACACCAGG AAGCAGCGCC 
GCCTCCTCGC CGATGTTCTC CATGTCGGGC GACGGCACCG ACGACGTCGC CATCCCCACG 
GTGTTCCTGT TCGCCCAGGA CGCCTCCAAG CTGCTGTTCG CGCTGAGCAG GGACCCCAGC 
ATCGAAGTCA CCATAGGCGA GTATAAAAAC GAAGGTGAGG CGTGGGCGCA CAGCGAGGAG 
GAAAGTATGT TTCATAAATT GAAAGTCTCC GTCCAGGAGT TTTTGAATAA ACACACCGGG 
ATTGCGTTCA GTAAGTCGGT CAAAGTTGGC GCGTTTAGGG CGAGTTTAGG GTCTGATAAA 
ATACGGATAA CGTACGAGGA GGGCGAGGAG GGGGGGAAGG TCGCCGGGGT GGGAGATACA 
GTGACCACGC CCCAGTGGGT GCAAGTCCGT AGGGATTTGC TCAAGTCGAT TATGACGTCA 
GAGGGGAAGG AATTGTACGT CCCTTTGAAT ATTTTGCGGA TTTATTATCA AACATTAAGT 
GATGATCCGA TGGAAGATAA GAGAGTGCAA GATACGGTCA AGCAGGCCGA GTGGTTGCTC 
AATCAACTGT CCATTGAATT GGCCAATAAG GACGCGTTGG TTAAACCGGA GGATGGGGTT 
AAGGTGTTGT CGGTATTAAC CGACTTGGGT CAGTTGGCTG GGAGCGATCT CGAGGATAAG 
GAAACACTAA AGGCGCTGAA TTCGATTCTG GAAAGTGTGG CCAAGGATGT TAAGTTCAAG 
AAGGAACATT TGGGTGCGGT TATGAAGAAG TTGCAAGAGG CGGATGATGA TTTGGTGGTC 
AGTGATGTTA AGCCCAAAGA GACGGACAGG GATTCTAGTG AAATTAATAG TAGTGATAGT 
GAACATATTC AGACGAAAAT CAGTCACGCC GTTGATGAGT TATGA