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hcn2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Summary

Gene Symbol hcn2
Entrez Gene ID 100487897
Full Name hyperpolarization activated cyclic nucleotide gated potassium channel 2
General protein information
Preferred Names

hyperpolarization activated cyclic nucleotide gated potassium channel 2

Names

potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Gene Type protein-coding
Organism Xenopus tropicalis(tropical clawed frog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_031905936.1 XP_031761796.1 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 isoform X1
XM_002939769.4 XP_002939815.3 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 isoform X2

Pathways from BioSystems


Homologies


Homo sapiens (human) HCN2 NP_001185.3
Gallus gallus (chicken) HCN2 XP_003642915.1
Xenopus tropicalis (tropical clawed frog) hcn2 XP_002939815.2
Canis lupus familiaris (dog) HCN2 XP_005642548.1
Rattus norvegicus (Norway rat) Hcn2 NP_446136.1
Pan troglodytes (chimpanzee) LOC467640 XP_003952669.1
Bos taurus (cattle) HCN2 XP_002689207.2
Mus musculus (house mouse) Hcn2 NP_032252.1
Danio rerio (zebrafish) hcn2b XP_685414.3

Gene Ontology

The following hcn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hcn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa42050 XM_002939769.4
Latest version!		 Xenopus tropicalis hyperpolarization activated cyclic nucleotide gated potassium channel 2 (hcn2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $510.30
$729.00
OXa48609 XM_031905936.1
Latest version!		 Xenopus tropicalis hyperpolarization activated cyclic nucleotide gated potassium channel 2 (hcn2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OXa42050
Clone ID Related Accession (Same CDS sequence) XM_002939769.4
Accession Version XM_002939769.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2709bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-17
Organism Xenopus tropicalis(tropical clawed frog)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939769.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACGGGC GGGGAGGCGG CGGCTGCAGC GCTGCGGGGA TGCGGGGAGT GGAGGTAGCA 
GCGGCGGCCG CGGAAGAACA ACTGCCCCGG GGTGTGGGTA GACGTGGGAC CCGGAGCCCT 
TGTAGCTCAG CAGGGACGCC GGGCACGGGC AAGGGCAGTC CCAATGGGGA GTGCAGGAGG 
GAGCTTTCCG AGACGCCGCC CCGGGAGCAC AAGGTGTCCT TCTCTACCGA GCCCCCGCGG 
GGGTCCTCAC CGGGCAAGGA GCCCGAAGAT GGGGATGGCG GAGAAGATGA AACCCGCTCT 
GGGTTCATGC AGCGCCAGCT TTCCGCCATG CTCCAGCCCG GGGTCAACAA GTTCTCCCTG 
CGGATGTTCG GCAGCCAAAA AGCGGTGGAA AGGGAACAGG AGAGGGTGAA ATCCGCGGGG 
GCTTGGATCA TACACCCCTA CAGCGACTTC AGATTCTATT GGGACTTCAC AATGCTGCTT 
TTCATGGTGG GCAATCTTAT CATCATTCCT GTGGGCATCA CCTTCTTTAA AGATGAGACC 
ACAACTCCAT GGATCGTGTT TAATGTGGTG TCGGACACCT TCTTCCTTAT GGACCTGGTT 
CTGAATTTCC GAACAGGGAT TGTGATTGAG GACAACACAG AAATAATACT AGACCCACAA 
AAGATTAAGC GCAAGTATCT GCGGACATGG TTTGTAGTGG ACTTTGTATC TTCAATTCCT 
GTGGATTACA TCTTCCTCAT TGTAGAGAAG GGTATTGACT CTGAAGTATA TAAGACAGCC 
AGAGCATTAA GAATTGTGAG ATTCACCAAA ATACTTAGTC TGTTGAGATT GCTTCGTCTC 
TCGAGACTTA TTCGATATAT ACATCAGTGG GAAGAGATTT TCCACATGAC TTATGACCTT 
GCCAGTGCAG TTATGCGGAT CTTTAACTTG ATTGCCATGA TGCTGTTGCT TTGCCATTGG 
GATGGCTGCC TACAGTTCCT TGTTCCTATG CTCCAAGATT TCCCAACTAA CTGTTGGGTG 
TCTATAAACA AGATGGTGAA TGATTCATGG AGTGAACTTT ACTCCTTTGC ACTCTTCAAA 
GCCATGAGCC ACATGCTGTG TATCGGCTAT GGGCGCCAAG CTCCAGAGAG CATGTCAGAT 
ATTTGGCTTA CCATGCTAAG CATGATCGTT GGAGCAACAT GCTATGCTAT GTTCATAGGA 
CATGCTACAG CTCTCATCCA ATCTTTGGAC TCCTCACGCA GGCAATATCA AGAAAAGTAC 
AAGCAGGTGG AGCAGTACAT GTCCTTTCAC AAGCTTCCTG CTGACTTTCG CCAAAGGCTT 
CATGATTATT ATGAGCATCG CTACCAAGGC AAGATGTTTG ATGAGGACAG TATCCTAGGA 
GAGCTGAATG AGCCCCTGCG AGAGGAAATA GTCAACTTTA ACTGCCGGAA ACTGGTAGCA 
TCCATGCCAC TGTTTGCCAA TGCTGACCCT TCTTTCGTGA CTGCCATGCT GACCAAGTTG 
AAGTTTGAGG TCTTTCAGCC CACAGATTAC ATTATTCGTG AGGGGACTAT TGGTAAAAAG 
ATGTATTTCA TCCAACATGG AGTGGTCAGC GTCCTCACCA AGGGCAACAA GGAGGTGAAG 
CTGTCAGATG GCTCCTACTT TGGAGAGATC TGTCTTCTAA CACGAGGCCG AAGGACAGCA 
AGTGTGCGTG CTGACACCTA CTGTCGTCTG TATTCGCTTT CTGTGGATCA CTTTAATGAG 
GTGCTGGAGG AATATCCCAT GATGAGACGT GCATTTGAGA CAGTGGCCAT AGACAGGCTG 
GATAGGATCG GAAAGAAGAA TTCTATACTA ATGCACAAGG TTCAGCATGA TTTAAACTCC 
GGTGTCTTTA ACAATAAGGA GAATGAAATT ATCCAGGAAA TAGTGAAGTA TGATCGGGAG 
ATGGTGCATC AAGCGGAACT AAACCAACAA CTCGCCGGTG GGATGTATGC CACTTGCAAT 
CCACCTTCTC CCCAACCACA GGTCACCTCT GCCATAGCCA CTCTACAACA GGCTGTGGCT 
ATGAGCTTCT GTCCCCCAAT GGCCAGCCCA CTAGTGGGAT CACCCCGCAT CATGAGACGC 
TTTCAGTATG CCCAAGGAGG ACCCAGCGCT TTCTCTTTGT CTCCAGCCTT GTTTCAGCCA 
CCACTTCAGC AAATGCAACA TTACCCTCCC CCACAAGTTT CCAGACAGCC TTCCTCTGTT 
GTGCCCAGTC CACCATCACA GTCCCCACGA GTGTCCCAGA ACTTTGCATA CCCAACAAAA 
GGTCAGACAG GCTCCCAGCT CTCCCTCACT CAGTCCGGGA CCTCTGCTTC TCCTTCCCCT 
CATAGGCTTT CTGTCCATAA GAGTACACAT TCTCTGCACT CTGGTACTCT TACCCGTGAG 
TCTCGTCCAC TCTCTGCCTC ACAGCCATCC CTACCCCAGG GGGCATCACA GCTGCGTAGC 
CCACCAGCAC CTCCTTTAGC TTCTGGGGGC TTAAGCTCCC AATTGGGCAA GGGAATGCCT 
GGTTCTCAAG CTGGGCCAAG GGTTCCTGTG CCAGCACGTC TGGCCCTTTC TCATCATTTG 
TCCTCTTCTA GTTCAGTTGG TGGTGTTTCG GACTCTGCAG GTGGAGCGTC ACTAGGAGGC 
AGGAAGGATT CAGTAGTGAG TGCCCCAGAT ACCGATCCTA TTAGGGGGCG GCTGTCTGCT 
AACTTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_002939815.3
CDS62..2770
Translation

Target ORF information:

RefSeq Version XM_002939769.4
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis hyperpolarization activated cyclic nucleotide gated potassium channel 2 (hcn2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_002939769.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGACGGGC GGGGAGGCGG CGGCTGCAGC GCTGCGGGGA TGCGGGGAGT GGAGGTAGCA 
GCGGCGGCCG CGGAAGAACA ACTGCCCCGG GGTGTGGGTA GACGTGGGAC CCGGAGCCCT 
TGTAGCTCAG CAGGGACGCC GGGCACGGGC AAGGGCAGTC CCAATGGGGA GTGCAGGAGG 
GAGCTTTCCG AGACGCCGCC CCGGGAGCAC AAGGTGTCCT TCTCTACCGA GCCCCCGCGG 
GGGTCCTCAC CGGGCAAGGA GCCCGAAGAT GGGGATGGCG GAGAAGATGA AACCCGCTCT 
GGGTTCATGC AGCGCCAGCT TTCCGCCATG CTCCAGCCCG GGGTCAACAA GTTCTCCCTG 
CGGATGTTCG GCAGCCAAAA AGCGGTGGAA AGGGAACAGG AGAGGGTGAA ATCCGCGGGG 
GCTTGGATCA TACACCCCTA CAGCGACTTC AGATTCTATT GGGACTTCAC AATGCTGCTT 
TTCATGGTGG GCAATCTTAT CATCATTCCT GTGGGCATCA CCTTCTTTAA AGATGAGACC 
ACAACTCCAT GGATCGTGTT TAATGTGGTG TCGGACACCT TCTTCCTTAT GGACCTGGTT 
CTGAATTTCC GAACAGGGAT TGTGATTGAG GACAACACAG AAATAATACT AGACCCACAA 
AAGATTAAGC GCAAGTATCT GCGGACATGG TTTGTAGTGG ACTTTGTATC TTCAATTCCT 
GTGGATTACA TCTTCCTCAT TGTAGAGAAG GGTATTGACT CTGAAGTATA TAAGACAGCC 
AGAGCATTAA GAATTGTGAG ATTCACCAAA ATACTTAGTC TGTTGAGATT GCTTCGTCTC 
TCGAGACTTA TTCGATATAT ACATCAGTGG GAAGAGATTT TCCACATGAC TTATGACCTT 
GCCAGTGCAG TTATGCGGAT CTTTAACTTG ATTGCCATGA TGCTGTTGCT TTGCCATTGG 
GATGGCTGCC TACAGTTCCT TGTTCCTATG CTCCAAGATT TCCCAACTAA CTGTTGGGTG 
TCTATAAACA AGATGGTGAA TGATTCATGG AGTGAACTTT ACTCCTTTGC ACTCTTCAAA 
GCCATGAGCC ACATGCTGTG TATCGGCTAT GGGCGCCAAG CTCCAGAGAG CATGTCAGAT 
ATTTGGCTTA CCATGCTAAG CATGATCGTT GGAGCAACAT GCTATGCTAT GTTCATAGGA 
CATGCTACAG CTCTCATCCA ATCTTTGGAC TCCTCACGCA GGCAATATCA AGAAAAGTAC 
AAGCAGGTGG AGCAGTACAT GTCCTTTCAC AAGCTTCCTG CTGACTTTCG CCAAAGGCTT 
CATGATTATT ATGAGCATCG CTACCAAGGC AAGATGTTTG ATGAGGACAG TATCCTAGGA 
GAGCTGAATG AGCCCCTGCG AGAGGAAATA GTCAACTTTA ACTGCCGGAA ACTGGTAGCA 
TCCATGCCAC TGTTTGCCAA TGCTGACCCT TCTTTCGTGA CTGCCATGCT GACCAAGTTG 
AAGTTTGAGG TCTTTCAGCC CACAGATTAC ATTATTCGTG AGGGGACTAT TGGTAAAAAG 
ATGTATTTCA TCCAACATGG AGTGGTCAGC GTCCTCACCA AGGGCAACAA GGAGGTGAAG 
CTGTCAGATG GCTCCTACTT TGGAGAGATC TGTCTTCTAA CACGAGGCCG AAGGACAGCA 
AGTGTGCGTG CTGACACCTA CTGTCGTCTG TATTCGCTTT CTGTGGATCA CTTTAATGAG 
GTGCTGGAGG AATATCCCAT GATGAGACGT GCATTTGAGA CAGTGGCCAT AGACAGGCTG 
GATAGGATCG GAAAGAAGAA TTCTATACTA ATGCACAAGG TTCAGCATGA TTTAAACTCC 
GGTGTCTTTA ACAATAAGGA GAATGAAATT ATCCAGGAAA TAGTGAAGTA TGATCGGGAG 
ATGGTGCATC AAGCGGAACT AAACCAACAA CTCGCCGGTG GGATGTATGC CACTTGCAAT 
CCACCTTCTC CCCAACCACA GGTCACCTCT GCCATAGCCA CTCTACAACA GGCTGTGGCT 
ATGAGCTTCT GTCCCCCAAT GGCCAGCCCA CTAGTGGGAT CACCCCGCAT CATGAGACGC 
TTTCAGTATG CCCAAGGAGG ACCCAGCGCT TTCTCTTTGT CTCCAGCCTT GTTTCAGCCA 
CCACTTCAGC AAATGCAACA TTACCCTCCC CCACAAGTTT CCAGACAGCC TTCCTCTGTT 
GTGCCCAGTC CACCATCACA GTCCCCACGA GTGTCCCAGA ACTTTGCATA CCCAACAAAA 
GGTCAGACAG GCTCCCAGCT CTCCCTCACT CAGTCCGGGA CCTCTGCTTC TCCTTCCCCT 
CATAGGCTTT CTGTCCATAA GAGTACACAT TCTCTGCACT CTGGTACTCT TACCCGTGAG 
TCTCGTCCAC TCTCTGCCTC ACAGCCATCC CTACCCCAGG GGGCATCACA GCTGCGTAGC 
CCACCAGCAC CTCCTTTAGC TTCTGGGGGC TTAAGCTCCC AATTGGGCAA GGGAATGCCT 
GGTTCTCAAG CTGGGCCAAG GGTTCCTGTG CCAGCACGTC TGGCCCTTTC TCATCATTTG 
TCCTCTTCTA GTTCAGTTGG TGGTGTTTCG GACTCTGCAG GTGGAGCGTC ACTAGGAGGC 
AGGAAGGATT CAGTAGTGAG TGCCCCAGAT ACCGATCCTA TTAGGGGGCG GCTGTCTGCT 
AACTTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa48609
Clone ID Related Accession (Same CDS sequence) XM_031905936.1
Accession Version XM_031905936.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-17
Organism Xenopus tropicalis(tropical clawed frog)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGACGGGC GGGGAGGCGG CGGCTGCAGC GCTGCGGGGA TGCGGGGAGT GGAGGTAGCA 
GCGGCGGCCG CGGAAGAACA ACTGCCCCGG GGTGTGGGTA GACGTGGGAC CCGGAGCCCT 
TGTAGCTCAG CAGGGACGCC GGGCACGGGC AAGGGCAGTC CCAATGGGGA GTGCAGGAGG 
GAGCTTTCCG AGACGCCGCC CCGGGAGCAC AAGGTGTCCT TCTCTACCGA GCCCCCGCGG 
GGGTCCTCAC CGGGCAAGGA GCCCGAAGAT GGGGATGGCG GAGAAGATGA AACCCGCTCT 
GGGTTCATGC AGCGCCAGCT TTCCGCCATG CTCCAGCCCG GGGTCAACAA GTTCTCCCTG 
CGGATGTTCG GCAGCCAAAA AGCGGTGGAA AGGGAACAGG AGAGGGTGAA ATCCGCGGGG 
GCTTGGATCA TACACCCCTA CAGCGACTTC AGATTCTATT GGGACTTCAC AATGCTGCTT 
TTCATGGTGG GCAATCTTAT CATCATTCCT GTGGGCATCA CCTTCTTTAA AGATGAGACC 
ACAACTCCAT GGATCGTGTT TAATGTGGTG TCGGACACCT TCTTCCTTAT GGACCTGGTT 
CTGAATTTCC GAACAGGGAT TGTGATTGAG GACAACACAG AAATAATACT AGACCCACAA 
AAGATTAAGC GCAAGTATCT GCGGACATGG TTTGTAGTGG ACTTTGTATC TTCAATTCCT 
GTGGATTACA TCTTCCTCAT TGTAGAGAAG GGTATTGACT CTGAAGTATA TAAGACAGCC 
AGAGCATTAA GAATTGTGAG ATTCACCAAA ATACTTAGTC TGTTGAGATT GCTTCGTCTC 
TCGAGACTTA TTCGATATAT ACATCAGTGG GAAGAGGTAT ATGTTGAAAA GCTTTGGACA 
AGGAGCGCAG GCCTGTTCTT CAAAAGTGTA TGTTTCAAAA TTTTCCACAT GACTTATGAC 
CTTGCCAGTG CAGTTATGCG GATCTTTAAC TTGATTGCCA TGATGCTGTT GCTTTGCCAT 
TGGGATGGCT GCCTACAGTT CCTTGTTCCT ATGCTCCAAG ATTTCCCAAC TAACTGTTGG 
GTGTCTATAA ACAAGATGGT GAATGATTCA TGGAGTGAAC TTTACTCCTT TGCACTCTTC 
AAAGCCATGA GCCACATGCT GTGTATCGGC TATGGGCGCC AAGCTCCAGA GAGCATGTCA 
GATATTTGGC TTACCATGCT AAGCATGATC GTTGGAGCAA CATGCTATGC TATGTTCATA 
GGACATGCTA CAGCTCTCAT CCAATCTTTG GACTCCTCAC GCAGGCAATA TCAAGAAAAG 
TACAAGCAGG TGGAGCAGTA CATGTCCTTT CACAAGCTTC CTGCTGACTT TCGCCAAAGG 
CTTCATGATT ATTATGAGCA TCGCTACCAA GGCAAGATGT TTGATGAGGA CAGTATCCTA 
GGAGAGCTGA ATGAGCCCCT GCGAGAGGAA ATAGTCAACT TTAACTGCCG GAAACTGGTA 
GCATCCATGC CACTGTTTGC CAATGCTGAC CCTTCTTTCG TGACTGCCAT GCTGACCAAG 
TTGAAGTTTG AGGTCTTTCA GCCCACAGAT TACATTATTC GTGAGGGGAC TATTGGTAAA 
AAGATGTATT TCATCCAACA TGGAGTGGTC AGCGTCCTCA CCAAGGGCAA CAAGGAGGTG 
AAGCTGTCAG ATGGCTCCTA CTTTGGAGAG ATCTGTCTTC TAACACGAGG CCGAAGGACA 
GCAAGTGTGC GTGCTGACAC CTACTGTCGT CTGTATTCGC TTTCTGTGGA TCACTTTAAT 
GAGGTGCTGG AGGAATATCC CATGATGAGA CGTGCATTTG AGACAGTGGC CATAGACAGG 
CTGGATAGGA TCGGAAAGAA GAATTCTATA CTAATGCACA AGGTTCAGCA TGATTTAAAC 
TCCGGTGTCT TTAACAATAA GGAGAATGAA ATTATCCAGG AAATAGTGAA GTATGATCGG 
GAGATGGTGC ATCAAGCGGA ACTAAACCAA CAACTCGCCG GTGGGATGTA TGCCACTTGC 
AATCCACCTT CTCCCCAACC ACAGGTCACC TCTGCCATAG CCACTCTACA ACAGGCTGTG 
GCTATGAGCT TCTGTCCCCC AATGGCCAGC CCACTAGTGG GATCACCCCG CATCATGAGA 
CGCTTTCAGT ATGCCCAAGG AGGACCCAGC GCTTTCTCTT TGTCTCCAGC CTTGTTTCAG 
CCACCACTTC AGCAAATGCA ACATTACCCT CCCCCACAAG TTTCCAGACA GCCTTCCTCT 
GTTGTGCCCA GTCCACCATC ACAGTCCCCA CGAGTGTCCC AGAACTTTGC ATACCCAACA 
AAAGGTCAGA CAGGCTCCCA GCTCTCCCTC ACTCAGTCCG GGACCTCTGC TTCTCCTTCC 
CCTCATAGGC TTTCTGTCCA TAAGAGTACA CATTCTCTGC ACTCTGGTAC TCTTACCCGT 
GAGTCTCGTC CACTCTCTGC CTCACAGCCA TCCCTACCCC AGGGGGCATC ACAGCTGCGT 
AGCCCACCAG CACCTCCTTT AGCTTCTGGG GGCTTAAGCT CCCAATTGGG CAAGGGAATG 
CCTGGTTCTC AAGCTGGGCC AAGGGTTCCT GTGCCAGCAC GTCTGGCCCT TTCTCATCAT 
TTGTCCTCTT CTAGTTCAGT TGGTGGTGTT TCGGACTCTG CAGGTGGAGC GTCACTAGGA 
GGCAGGAAGG ATTCAGTAGT GAGTGCCCCA GATACCGATC CTATTAGGGG GCGGCTGTCT 
GCTAACTTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_031761796.1
CDS69..2840
Translation

Target ORF information:

RefSeq Version XM_031905936.1
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis hyperpolarization activated cyclic nucleotide gated potassium channel 2 (hcn2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_031905936.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGACGGGC GGGGAGGCGG CGGCTGCAGC GCTGCGGGGA TGCGGGGAGT GGAGGTAGCA 
GCGGCGGCCG CGGAAGAACA ACTGCCCCGG GGTGTGGGTA GACGTGGGAC CCGGAGCCCT 
TGTAGCTCAG CAGGGACGCC GGGCACGGGC AAGGGCAGTC CCAATGGGGA GTGCAGGAGG 
GAGCTTTCCG AGACGCCGCC CCGGGAGCAC AAGGTGTCCT TCTCTACCGA GCCCCCGCGG 
GGGTCCTCAC CGGGCAAGGA GCCCGAAGAT GGGGATGGCG GAGAAGATGA AACCCGCTCT 
GGGTTCATGC AGCGCCAGCT TTCCGCCATG CTCCAGCCCG GGGTCAACAA GTTCTCCCTG 
CGGATGTTCG GCAGCCAAAA AGCGGTGGAA AGGGAACAGG AGAGGGTGAA ATCCGCGGGG 
GCTTGGATCA TACACCCCTA CAGCGACTTC AGATTCTATT GGGACTTCAC AATGCTGCTT 
TTCATGGTGG GCAATCTTAT CATCATTCCT GTGGGCATCA CCTTCTTTAA AGATGAGACC 
ACAACTCCAT GGATCGTGTT TAATGTGGTG TCGGACACCT TCTTCCTTAT GGACCTGGTT 
CTGAATTTCC GAACAGGGAT TGTGATTGAG GACAACACAG AAATAATACT AGACCCACAA 
AAGATTAAGC GCAAGTATCT GCGGACATGG TTTGTAGTGG ACTTTGTATC TTCAATTCCT 
GTGGATTACA TCTTCCTCAT TGTAGAGAAG GGTATTGACT CTGAAGTATA TAAGACAGCC 
AGAGCATTAA GAATTGTGAG ATTCACCAAA ATACTTAGTC TGTTGAGATT GCTTCGTCTC 
TCGAGACTTA TTCGATATAT ACATCAGTGG GAAGAGGTAT ATGTTGAAAA GCTTTGGACA 
AGGAGCGCAG GCCTGTTCTT CAAAAGTGTA TGTTTCAAAA TTTTCCACAT GACTTATGAC 
CTTGCCAGTG CAGTTATGCG GATCTTTAAC TTGATTGCCA TGATGCTGTT GCTTTGCCAT 
TGGGATGGCT GCCTACAGTT CCTTGTTCCT ATGCTCCAAG ATTTCCCAAC TAACTGTTGG 
GTGTCTATAA ACAAGATGGT GAATGATTCA TGGAGTGAAC TTTACTCCTT TGCACTCTTC 
AAAGCCATGA GCCACATGCT GTGTATCGGC TATGGGCGCC AAGCTCCAGA GAGCATGTCA 
GATATTTGGC TTACCATGCT AAGCATGATC GTTGGAGCAA CATGCTATGC TATGTTCATA 
GGACATGCTA CAGCTCTCAT CCAATCTTTG GACTCCTCAC GCAGGCAATA TCAAGAAAAG 
TACAAGCAGG TGGAGCAGTA CATGTCCTTT CACAAGCTTC CTGCTGACTT TCGCCAAAGG 
CTTCATGATT ATTATGAGCA TCGCTACCAA GGCAAGATGT TTGATGAGGA CAGTATCCTA 
GGAGAGCTGA ATGAGCCCCT GCGAGAGGAA ATAGTCAACT TTAACTGCCG GAAACTGGTA 
GCATCCATGC CACTGTTTGC CAATGCTGAC CCTTCTTTCG TGACTGCCAT GCTGACCAAG 
TTGAAGTTTG AGGTCTTTCA GCCCACAGAT TACATTATTC GTGAGGGGAC TATTGGTAAA 
AAGATGTATT TCATCCAACA TGGAGTGGTC AGCGTCCTCA CCAAGGGCAA CAAGGAGGTG 
AAGCTGTCAG ATGGCTCCTA CTTTGGAGAG ATCTGTCTTC TAACACGAGG CCGAAGGACA 
GCAAGTGTGC GTGCTGACAC CTACTGTCGT CTGTATTCGC TTTCTGTGGA TCACTTTAAT 
GAGGTGCTGG AGGAATATCC CATGATGAGA CGTGCATTTG AGACAGTGGC CATAGACAGG 
CTGGATAGGA TCGGAAAGAA GAATTCTATA CTAATGCACA AGGTTCAGCA TGATTTAAAC 
TCCGGTGTCT TTAACAATAA GGAGAATGAA ATTATCCAGG AAATAGTGAA GTATGATCGG 
GAGATGGTGC ATCAAGCGGA ACTAAACCAA CAACTCGCCG GTGGGATGTA TGCCACTTGC 
AATCCACCTT CTCCCCAACC ACAGGTCACC TCTGCCATAG CCACTCTACA ACAGGCTGTG 
GCTATGAGCT TCTGTCCCCC AATGGCCAGC CCACTAGTGG GATCACCCCG CATCATGAGA 
CGCTTTCAGT ATGCCCAAGG AGGACCCAGC GCTTTCTCTT TGTCTCCAGC CTTGTTTCAG 
CCACCACTTC AGCAAATGCA ACATTACCCT CCCCCACAAG TTTCCAGACA GCCTTCCTCT 
GTTGTGCCCA GTCCACCATC ACAGTCCCCA CGAGTGTCCC AGAACTTTGC ATACCCAACA 
AAAGGTCAGA CAGGCTCCCA GCTCTCCCTC ACTCAGTCCG GGACCTCTGC TTCTCCTTCC 
CCTCATAGGC TTTCTGTCCA TAAGAGTACA CATTCTCTGC ACTCTGGTAC TCTTACCCGT 
GAGTCTCGTC CACTCTCTGC CTCACAGCCA TCCCTACCCC AGGGGGCATC ACAGCTGCGT 
AGCCCACCAG CACCTCCTTT AGCTTCTGGG GGCTTAAGCT CCCAATTGGG CAAGGGAATG 
CCTGGTTCTC AAGCTGGGCC AAGGGTTCCT GTGCCAGCAC GTCTGGCCCT TTCTCATCAT 
TTGTCCTCTT CTAGTTCAGT TGGTGGTGTT TCGGACTCTG CAGGTGGAGC GTCACTAGGA 
GGCAGGAAGG ATTCAGTAGT GAGTGCCCCA GATACCGATC CTATTAGGGG GCGGCTGTCT 
GCTAACTTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed