trhde cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following trhde gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trhde cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa54716 XM_002939942.5
Latest version!
Xenopus tropicalis thyrotropin releasing hormone degrading enzyme (trhde), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa07778 XM_002939942.4
Latest version!
Xenopus tropicalis thyrotropin releasing hormone degrading enzyme (trhde), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa54716
    Clone ID Related Accession (Same CDS sequence) XM_002939942.5
    Accession Version XM_002939942.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product thyrotropin-releasing hormone-degrading ectoenzyme
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939942.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCGGGG ACAGTGAGAA GCACAAGAAG AACAGCTTAT TTCCCAGTCC CATGTTAATA 
    GAAGACACCG GGATGAGGAA CGGGAGAGGG CTCCAGGACC AGTGGGCTGA TACTGTGGCA
    GTGAGAACCA GGACCACCGA AAGGCAGATC ACTGTTCATA GGAAGCTGGT CCTGGCATTT
    GCCATTTCTA TTCTGGCTTT GATCGTTGTC ACTATTATTG CGGTCCTGCT TAGCGTTCGC
    TTTGAGGAAT GTACAGACGG CTCCACTAGG GTCATTAATA GCAGCATCCT GGAGGCAGCC
    AGGTTCAACC AAAACCTAGA GCTAGACAAG GATTCCGGGG AAGGGACCCA GCGTCCGCCA
    GTGAAAGACC CAGAGGAGGA ATGGAAGCCT TGGACTTACC TGAGGCTACC GACTCACCTG
    AGACCCCTAC ACTACAATCT CATGGTCACC GCATTCATGA ACAACTTTAC TTTCTGCGGA
    GAAGTCAATG TGGAGATCGA GTGTCTGAAC TCCAGCAGAT ACATAGTGGT CCATGCCCAC
    AGGCTGACTG TGGAGAGGGT CCAGGTGGCG GAGGATCGAG TGGCTGGGGA AGTTAAAGTT
    TCTCATTATT TTCCTTATCC GCAAAACCAA GTTTTGGTGG TGGTTTTGAA CAGATCCCTG
    GAGGCCCAGA AAAGTTATAA CCTGAAGATC ATTTATAGTG CTATCATAGA GAATGAGCTC
    CTTGGCTTCT TCCGGAGCTC CTATGTGCTG CATGGAGAGA GAAGATCCCT TGCTGTTACC
    CAGTTTTCTC CAATCCATGC AAGAAAAGCC TTCCCCTGTT TTGATGAGCC TATCTACAAG
    GCAACATTCA AAATAAGTAT TAGGCATCAG ACCTCTTACC TTTCTTTGTC AAATATGCCT
    GTGGAAACTT CTGTTTTTGA AGAAGGTGGA TGGGTTACAG ATCATTTTTC ACAAACTCCT
    TTAATGTCTA CCTACTACCT TGCCTGGGCA GTGTGCAATT TTACCTTCAA GGAAGCGGTA
    ACACAGAGTG GTGTTGTTGT GAGATTGTAT GCACGTCCTG ATGCCATCAG AAGAGGATCT
    GGAGATTATG CCCTTAATAT CACAAAAAGA CTCCTAGAAT TTTATGAAGA TTACTTCAGA
    GTCCCATACT CATTACCAAA ATTAGATTTA TTAGCTGTGC CTAAACACCC TTATGCTGCT
    ATGGAAAACT GGGGTCTCAG TGTATTTGTG GAGCAAAGAA TACTCCTTGA TCCAAGCATT
    TCTTCCATTT CCTATTTACT TGATGTTACT ATGGTCATTG TACATGAAAT TTGCCACCAG
    TGGTTTGGAG ATCTGGTGAC ACCTATTTGG TGGGAAGATG TATGGTTAAA AGAAGGTCTT
    GCTCACTATT TTGAATTTGT TGGAACGGAC TACCTCTATC CGGGATGGAA CATGGAAAAG
    CAAAGATTTC TGACAGATGT CTTACATGAG GTCATGCTGT TAGATGGATT GGCTAGTTCG
    CATCCAGTTT CTCAAGAGGT TTTGAAGGCT ACAGATATTG ACCGTGTGTT TGACTGGATT
    GCTTATAAAA AGGGGGCAGC CCTAATTCGA ATGCTGGCTA ATTTTATGGG TCATAAAGTG
    TTTCAGAGAG GACTACAAGA TTATTTAACA ACTCATAAAT ATGGCAATGC TGCACGAAAT
    GATCTCTGGA ACACACTCAC CAAGGTTCTA AAGAATGAAG GTAAATTACT GAATATCCGG
    GAGGTAATGG ATCAGTGGAC ACTTCAGATG GGCTATCCTG TAATAACAAT CCTAAGAAAT
    GATACAACAG ATGACATCAT AACAATTACT CAGGAGCACT TCCTATACAA TATAGATAAA
    CGGACTAGAG ACAAGGAGTT CAGAAATAAA AGCTCGCTTT GGCAGATTCC ATTAACGATT
    GCAGTAGGAA ATACAAGTCA CATTTCTCCC CAGGCAATTA TATGGGTGTC TAACAAATCA
    GAATACCACC GAATTCCCTC TTTGGATGAA AGTAATTGGG TACTGGGAAA CCTTGATCAA
    ATTGGCTACT TCAGGGTTAA CTATGATGTC CGGAACTGGA GATTACTAAC TGACCAGTTA
    ATGAGGAACT CTGAGGTTAT TTCTGTCAGC AATCGAGCTG CACTGATCGA TGATGCTTTT
    AATTTAGCAA GAGCTGGATA TCTGACTCAG AATATTCCTC TGGAGATCAT CAGATACCTT
    TCTGAAGAGA AGGATTTTCT TCCCTGGCAT GCAGCAAGCC GAGCTCTTTA TCCTCTAGAT
    AAATTGCTGG ACCGAACGGA GGACTACAGT ATATTCAGTG AATATATACT GAAACAAGTA
    TCTACAATGT ACTTTCGTCT AGGATGGCCA ACAAATGGAC AGAATGGATC AGCAGTTCCA
    ACATCCTACC AGACAGAGGA GCTTAGACGA GAAGTCATTA TGTTAGCCTG TAGTTTTGGG
    AATAAACAGT GTCACCAGAA AGCAGCAACT ATGATATCTG ACTGGATCTC CAGCAACAGA
    AACCGAATTC CTCCAAATGT CCGTGACATA GTCTATTGCA CCGGAGTATC CTTGATGGAT
    GAAGATGTAT GGGAATTCAT TTGGATGAAA TTTCATTCTT CCACAGCTGT ATCAGAAAAG
    AAAATTCTAT TAGAAGCTCT GACCTGCAGT GATAATAGAA ACCTCCTGAA TAGGCTTCTG
    AATCTCTCCT TGAATTCAGA AGTAGTACTG GATCAAGATG CCATTGATGT CATTATTCAT
    GTGGCAAGAA ACCCACATGG AAGAGATTTA GCTTGGAAAT TCTTCAGAGA CAAATGGAAG
    ATTTTAAATG CTAGGTATGG AGAAGCATTG TTTATGAATT CAAAGCTCAT TAGTGGAGTA
    ACTGAATTTC TTAATACAGA AGAAGAACTA AATGAGCTTA AAGATTTCAT ACAACAGCAC
    AAAGGAGGAT CGGCTGTGGC TTCTCTGTCT CGTGCTACGG AGACTGTCGA TGCAAATGTA
    CGTTGGCAAC ATCTATACAG AGATGAACTC TTTCAGTGGC TGAGAAAAAC AGTGAAACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002939988.3
    CDS808..3870
    Translation

    Target ORF information:

    RefSeq Version XM_002939942.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis thyrotropin releasing hormone degrading enzyme (trhde), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002939942.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCGGGG ACAGTGAGAA GCACAAGAAG AACAGCTTAT TTCCCAGTCC CATGTTAATA 
    GAAGACACCG GGATGAGGAA CGGGAGAGGG CTCCAGGACC AGTGGGCTGA TACTGTGGCA
    GTGAGAACCA GGACCACCGA AAGGCAGATC ACTGTTCATA GGAAGCTGGT CCTGGCATTT
    GCCATTTCTA TTCTGGCTTT GATCGTTGTC ACTATTATTG CGGTCCTGCT TAGCGTTCGC
    TTTGAGGAAT GTACAGACGG CTCCACTAGG GTCATTAATA GCAGCATCCT GGAGGCAGCC
    AGGTTCAACC AAAACCTAGA GCTAGACAAG GATTCCGGGG AAGGGACCCA GCGTCCGCCA
    GTGAAAGACC CAGAGGAGGA ATGGAAGCCT TGGACTTACC TGAGGCTACC GACTCACCTG
    AGACCCCTAC ACTACAATCT CATGGTCACC GCATTCATGA ACAACTTTAC TTTCTGCGGA
    GAAGTCAATG TGGAGATCGA GTGTCTGAAC TCCAGCAGAT ACATAGTGGT CCATGCCCAC
    AGGCTGACTG TGGAGAGGGT CCAGGTGGCG GAGGATCGAG TGGCTGGGGA AGTTAAAGTT
    TCTCATTATT TTCCTTATCC GCAAAACCAA GTTTTGGTGG TGGTTTTGAA CAGATCCCTG
    GAGGCCCAGA AAAGTTATAA CCTGAAGATC ATTTATAGTG CTATCATAGA GAATGAGCTC
    CTTGGCTTCT TCCGGAGCTC CTATGTGCTG CATGGAGAGA GAAGATCCCT TGCTGTTACC
    CAGTTTTCTC CAATCCATGC AAGAAAAGCC TTCCCCTGTT TTGATGAGCC TATCTACAAG
    GCAACATTCA AAATAAGTAT TAGGCATCAG ACCTCTTACC TTTCTTTGTC AAATATGCCT
    GTGGAAACTT CTGTTTTTGA AGAAGGTGGA TGGGTTACAG ATCATTTTTC ACAAACTCCT
    TTAATGTCTA CCTACTACCT TGCCTGGGCA GTGTGCAATT TTACCTTCAA GGAAGCGGTA
    ACACAGAGTG GTGTTGTTGT GAGATTGTAT GCACGTCCTG ATGCCATCAG AAGAGGATCT
    GGAGATTATG CCCTTAATAT CACAAAAAGA CTCCTAGAAT TTTATGAAGA TTACTTCAGA
    GTCCCATACT CATTACCAAA ATTAGATTTA TTAGCTGTGC CTAAACACCC TTATGCTGCT
    ATGGAAAACT GGGGTCTCAG TGTATTTGTG GAGCAAAGAA TACTCCTTGA TCCAAGCATT
    TCTTCCATTT CCTATTTACT TGATGTTACT ATGGTCATTG TACATGAAAT TTGCCACCAG
    TGGTTTGGAG ATCTGGTGAC ACCTATTTGG TGGGAAGATG TATGGTTAAA AGAAGGTCTT
    GCTCACTATT TTGAATTTGT TGGAACGGAC TACCTCTATC CGGGATGGAA CATGGAAAAG
    CAAAGATTTC TGACAGATGT CTTACATGAG GTCATGCTGT TAGATGGATT GGCTAGTTCG
    CATCCAGTTT CTCAAGAGGT TTTGAAGGCT ACAGATATTG ACCGTGTGTT TGACTGGATT
    GCTTATAAAA AGGGGGCAGC CCTAATTCGA ATGCTGGCTA ATTTTATGGG TCATAAAGTG
    TTTCAGAGAG GACTACAAGA TTATTTAACA ACTCATAAAT ATGGCAATGC TGCACGAAAT
    GATCTCTGGA ACACACTCAC CAAGGTTCTA AAGAATGAAG GTAAATTACT GAATATCCGG
    GAGGTAATGG ATCAGTGGAC ACTTCAGATG GGCTATCCTG TAATAACAAT CCTAAGAAAT
    GATACAACAG ATGACATCAT AACAATTACT CAGGAGCACT TCCTATACAA TATAGATAAA
    CGGACTAGAG ACAAGGAGTT CAGAAATAAA AGCTCGCTTT GGCAGATTCC ATTAACGATT
    GCAGTAGGAA ATACAAGTCA CATTTCTCCC CAGGCAATTA TATGGGTGTC TAACAAATCA
    GAATACCACC GAATTCCCTC TTTGGATGAA AGTAATTGGG TACTGGGAAA CCTTGATCAA
    ATTGGCTACT TCAGGGTTAA CTATGATGTC CGGAACTGGA GATTACTAAC TGACCAGTTA
    ATGAGGAACT CTGAGGTTAT TTCTGTCAGC AATCGAGCTG CACTGATCGA TGATGCTTTT
    AATTTAGCAA GAGCTGGATA TCTGACTCAG AATATTCCTC TGGAGATCAT CAGATACCTT
    TCTGAAGAGA AGGATTTTCT TCCCTGGCAT GCAGCAAGCC GAGCTCTTTA TCCTCTAGAT
    AAATTGCTGG ACCGAACGGA GGACTACAGT ATATTCAGTG AATATATACT GAAACAAGTA
    TCTACAATGT ACTTTCGTCT AGGATGGCCA ACAAATGGAC AGAATGGATC AGCAGTTCCA
    ACATCCTACC AGACAGAGGA GCTTAGACGA GAAGTCATTA TGTTAGCCTG TAGTTTTGGG
    AATAAACAGT GTCACCAGAA AGCAGCAACT ATGATATCTG ACTGGATCTC CAGCAACAGA
    AACCGAATTC CTCCAAATGT CCGTGACATA GTCTATTGCA CCGGAGTATC CTTGATGGAT
    GAAGATGTAT GGGAATTCAT TTGGATGAAA TTTCATTCTT CCACAGCTGT ATCAGAAAAG
    AAAATTCTAT TAGAAGCTCT GACCTGCAGT GATAATAGAA ACCTCCTGAA TAGGCTTCTG
    AATCTCTCCT TGAATTCAGA AGTAGTACTG GATCAAGATG CCATTGATGT CATTATTCAT
    GTGGCAAGAA ACCCACATGG AAGAGATTTA GCTTGGAAAT TCTTCAGAGA CAAATGGAAG
    ATTTTAAATG CTAGGTATGG AGAAGCATTG TTTATGAATT CAAAGCTCAT TAGTGGAGTA
    ACTGAATTTC TTAATACAGA AGAAGAACTA AATGAGCTTA AAGATTTCAT ACAACAGCAC
    AAAGGAGGAT CGGCTGTGGC TTCTCTGTCT CGTGCTACGG AGACTGTCGA TGCAAATGTA
    CGTTGGCAAC ATCTATACAG AGATGAACTC TTTCAGTGGC TGAGAAAAAC AGTGAAACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07778
    Clone ID Related Accession (Same CDS sequence) XM_002939942.4
    Accession Version XM_002939942.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product thyrotropin-releasing hormone-degrading ectoenzyme
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939942.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCGGGG ACAGTGAGAA GCAAAAGAAG AACAGCTTAT TTCCCAGTCC CATGTTAATA 
    GAAGACACCG GGATGAGGAA CGGGAGAGGG CTCCAGGACC AGTGGGCTGA TACTGTGGCG
    GTGAGAACCA GGACCACCGA AAGGCAGATC ACTGTTCATA GGAAGCTGGT CCTGGCATTT
    GCCATTTCTA TTCTGGCTTT GATCGTTGTC ACTATTATTG CGGTCCTGCT TAGCGTTCGC
    TTTGAGGAAT GTACAGACGG CTCCACTAGG GTCATTAATA GCAGCGTCCT GGAGGCAGCC
    AGGTTCAACC AAAACCTAGA GCTAGACAAG GATTACGGGG AAGGGACCCA GCGTCCGCCA
    GTGAAAGACC CAGAGGAGGA ATGGAAGCCT TGGACTTACC TGAGGCTACC GACTCACCTG
    AGACCCCTAC ACTACAATCT CATGGTCACC GCATTCATGA ACAACTTTAC TTTCTGCGGA
    GAAGTCAATG TGGAGATCGA GTGTCTGAAC TCCAGCAGAT ACATAGTGGT CCATGCCCAC
    AGGCTGACTG TGGAGAGGGT CCAGGTGGCG GAGGATCGAG TGGCTGGGGA AGTTAAAGTT
    TCTCATTATT TTCCTTATCC GCAAAACCAA GTTTTGGTGG TGGTTTTGAA CAGATCCCTG
    GAGGCCCAGA AAAGTTATAA CCTGAAGATC ATTTATAGTG CTATCATAGA GAATGAGCTC
    CTTGGCTTCT TCCGGAGCTC CTATGTGCTG CATGGAGAGA GAAGATCCCT TGCTGTTACC
    CAGTTTTCTC CAATCCATGC AAGAAAAGCC TTCCCCTGTT TTGATGAGCC CATCTACAAG
    GCAACATTCA AAATAAGTAT TAGGCATCAG ACCTCTTACC TTTCTTTGTC AAATATGCCT
    GTGGAAACTT CTGTTTTTGA AGAAGGTGGA TGGGTTACAG ATCATTTTTC ACAAACTCCT
    TTAATGTCTA CCTACTACCT TGCCTGGGCA GTGTGCAATT TTACCTTCAA GGAAGCTGTA
    ACACAGAGTG GTGTTGTTGT GAGATTGTAT GCACGTCCTG ATGCCATCAG AAGAGGATCT
    GGAGATTATG CCCTTAATAT CACAAAAAGA CTCCTAGAAT TTTATGAAGA TTACTTCAGA
    GTCCCATACT CATTACCAAA ATTAGATTTA TTAGCTGTGC CTAAACACCC TTATGCTGCT
    ATGGAAAACT GGGGTCTCAG TGTATTTGTG GAGCAAAGAA TACTCCTTGA TCCAAGCATT
    TCTTCCATTT CCTATTTACT TGATGTTACT ATGGTCATTG TACATGAAAT TTGCCACCAG
    TGGTTTGGAG ATCTGGTGAC ACCTATTTGG TGGGAAGATG TATGGTTAAA AGAAGGTCTT
    GCTCACTATT TTGAATTTGT TGGAACGGAC TACCTCTATC CGGGATGGAA CATGGAAAAG
    CAAAGATTTC TGACAGATGT CTTACATGAG GTCATGCTGT TAGATGGATT GGCTAGTTCG
    CATCCAGTTT CTCAAGAGGT TTTGAAGGCT ACAGATATTG ACCGTGTGTT TGACTGGATT
    GCTTATAAAA AGGGGGCAGC CCTAATTCGA ATGCTGGCTA ATTTTATGGG TCATAAAGTG
    TTTCAGAGAG GACTACAAGA TTATTTAACA ACTCATAAAT ATGGCAATGC TGCACGAAAT
    GATCTCTGGA ACACACTCAC CAAGGTTCTA AAGAATGAAG GTAAATTACT GAATATCCGG
    GAGGTAATGG ATCAGTGGAC ACTTCAGATG GGCTATCCTG TAATAACAAT CCTAAGAAAT
    GATACAACAG ATGACATCAT AACAATTACT CAGGAGCACT TCCTATACAA TATAGATAAA
    CGGACTAGAG ACAAGGAGTT CAGAAATAAA AGCTCGCTTT GGCAGATTCC ATTAACGATT
    GCAGTAGGAA ATACAAGTCA CATTTCTCCC CAGGCAATTA TATGGGTGTC TAACAAATCA
    GAATACCACC GAATTCCCTC TTTGGATGAA AGTAATTGGG TACTGGGAAA CCTTGATCAA
    ATTGGCTACT TCAGGGTTAA CTATGATGTC CGGAACTGGA GATTACTAAC TGACCAGTTA
    ATGAGGAACT CTGAGGTTAT TTCTGTCAGC AATCGAGCTG CACTGATCGA TGATGCTTTT
    AATTTAGCAA GAGCTGGATA TCTGACTCAG AATATTCCTC TGGAGATCAT CAGATACCTT
    TCTGAAGAGA AGGATTTTCT TCCCTGGCAT GCAGCAAGCC GAGCTCTTTA TCCTCTAGAT
    AAATTGCTGG ACCGAACGGA GGACTACAGT ATATTCAGTG AATATATACT GAAACAAGTA
    TCTACAATGT ACTTTCGTCT AGGATGGCCA ACAAATGGAC AGAATGGATC AGCAGTTCCA
    ACATCCTACC AGACAGAGGA GCTTAGACGA GAAGTCATTA TGTTAGCCTG TAGTTTTGGG
    AATAAACAGT GTCACCAGAA AGCAGCAACT ATGATATCTG ACTGGATCTC CAGCAACAGA
    AACCGAATTC CTCCAAATGT CCGTGACATA GTCTATTGCA CCGGAGTATC CTTGATGGAT
    GAAGATGTAT GGGAATTCAT TTGGATGAAA TTTCATTCTT CCACAGCTGT ATCAGAAAAG
    AAAATTCTAT TAGAAGCTCT GACCTGCAGT GATAATAGAA ACCTCCTGAA TAGGCTTTTG
    AATCTCTCCT TGAATTCAGA AGTAGTACTG GATCAAGATG CCATTGATGT CATTATTCAT
    GTGGCAAGAA ACCCACATGG AAGAGATTTA GCTTGGAAAT TCTTCAGAGA CAAATGGAAG
    ATTTTAAATG CTAGGTATGG AGAAGCATTG TTTATGAATT CAAAGCTCAT TAGTGGAGTA
    ACTGAATTTC TTAATACAGA AGAAGAACTA AATGAGCTTA AAGATTTCAT ACAACAGCAC
    AAAGGAGGAT CGGCTGTGGC TTCTCTGTCT CGTGCTACGG AGACTGTCGA TGCAAATGTA
    CGTTGGCAAC ATCTATACAG AGATGAACTC TTTCAGTGGC TGAGAAAAAC AGTGAAACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002939988.2
    CDS3848..6910
    Translation

    Target ORF information:

    RefSeq Version XM_002939942.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis thyrotropin releasing hormone degrading enzyme (trhde), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002939942.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCCGGGG ACAGTGAGAA GCAAAAGAAG AACAGCTTAT TTCCCAGTCC CATGTTAATA 
    GAAGACACCG GGATGAGGAA CGGGAGAGGG CTCCAGGACC AGTGGGCTGA TACTGTGGCG
    GTGAGAACCA GGACCACCGA AAGGCAGATC ACTGTTCATA GGAAGCTGGT CCTGGCATTT
    GCCATTTCTA TTCTGGCTTT GATCGTTGTC ACTATTATTG CGGTCCTGCT TAGCGTTCGC
    TTTGAGGAAT GTACAGACGG CTCCACTAGG GTCATTAATA GCAGCGTCCT GGAGGCAGCC
    AGGTTCAACC AAAACCTAGA GCTAGACAAG GATTACGGGG AAGGGACCCA GCGTCCGCCA
    GTGAAAGACC CAGAGGAGGA ATGGAAGCCT TGGACTTACC TGAGGCTACC GACTCACCTG
    AGACCCCTAC ACTACAATCT CATGGTCACC GCATTCATGA ACAACTTTAC TTTCTGCGGA
    GAAGTCAATG TGGAGATCGA GTGTCTGAAC TCCAGCAGAT ACATAGTGGT CCATGCCCAC
    AGGCTGACTG TGGAGAGGGT CCAGGTGGCG GAGGATCGAG TGGCTGGGGA AGTTAAAGTT
    TCTCATTATT TTCCTTATCC GCAAAACCAA GTTTTGGTGG TGGTTTTGAA CAGATCCCTG
    GAGGCCCAGA AAAGTTATAA CCTGAAGATC ATTTATAGTG CTATCATAGA GAATGAGCTC
    CTTGGCTTCT TCCGGAGCTC CTATGTGCTG CATGGAGAGA GAAGATCCCT TGCTGTTACC
    CAGTTTTCTC CAATCCATGC AAGAAAAGCC TTCCCCTGTT TTGATGAGCC CATCTACAAG
    GCAACATTCA AAATAAGTAT TAGGCATCAG ACCTCTTACC TTTCTTTGTC AAATATGCCT
    GTGGAAACTT CTGTTTTTGA AGAAGGTGGA TGGGTTACAG ATCATTTTTC ACAAACTCCT
    TTAATGTCTA CCTACTACCT TGCCTGGGCA GTGTGCAATT TTACCTTCAA GGAAGCTGTA
    ACACAGAGTG GTGTTGTTGT GAGATTGTAT GCACGTCCTG ATGCCATCAG AAGAGGATCT
    GGAGATTATG CCCTTAATAT CACAAAAAGA CTCCTAGAAT TTTATGAAGA TTACTTCAGA
    GTCCCATACT CATTACCAAA ATTAGATTTA TTAGCTGTGC CTAAACACCC TTATGCTGCT
    ATGGAAAACT GGGGTCTCAG TGTATTTGTG GAGCAAAGAA TACTCCTTGA TCCAAGCATT
    TCTTCCATTT CCTATTTACT TGATGTTACT ATGGTCATTG TACATGAAAT TTGCCACCAG
    TGGTTTGGAG ATCTGGTGAC ACCTATTTGG TGGGAAGATG TATGGTTAAA AGAAGGTCTT
    GCTCACTATT TTGAATTTGT TGGAACGGAC TACCTCTATC CGGGATGGAA CATGGAAAAG
    CAAAGATTTC TGACAGATGT CTTACATGAG GTCATGCTGT TAGATGGATT GGCTAGTTCG
    CATCCAGTTT CTCAAGAGGT TTTGAAGGCT ACAGATATTG ACCGTGTGTT TGACTGGATT
    GCTTATAAAA AGGGGGCAGC CCTAATTCGA ATGCTGGCTA ATTTTATGGG TCATAAAGTG
    TTTCAGAGAG GACTACAAGA TTATTTAACA ACTCATAAAT ATGGCAATGC TGCACGAAAT
    GATCTCTGGA ACACACTCAC CAAGGTTCTA AAGAATGAAG GTAAATTACT GAATATCCGG
    GAGGTAATGG ATCAGTGGAC ACTTCAGATG GGCTATCCTG TAATAACAAT CCTAAGAAAT
    GATACAACAG ATGACATCAT AACAATTACT CAGGAGCACT TCCTATACAA TATAGATAAA
    CGGACTAGAG ACAAGGAGTT CAGAAATAAA AGCTCGCTTT GGCAGATTCC ATTAACGATT
    GCAGTAGGAA ATACAAGTCA CATTTCTCCC CAGGCAATTA TATGGGTGTC TAACAAATCA
    GAATACCACC GAATTCCCTC TTTGGATGAA AGTAATTGGG TACTGGGAAA CCTTGATCAA
    ATTGGCTACT TCAGGGTTAA CTATGATGTC CGGAACTGGA GATTACTAAC TGACCAGTTA
    ATGAGGAACT CTGAGGTTAT TTCTGTCAGC AATCGAGCTG CACTGATCGA TGATGCTTTT
    AATTTAGCAA GAGCTGGATA TCTGACTCAG AATATTCCTC TGGAGATCAT CAGATACCTT
    TCTGAAGAGA AGGATTTTCT TCCCTGGCAT GCAGCAAGCC GAGCTCTTTA TCCTCTAGAT
    AAATTGCTGG ACCGAACGGA GGACTACAGT ATATTCAGTG AATATATACT GAAACAAGTA
    TCTACAATGT ACTTTCGTCT AGGATGGCCA ACAAATGGAC AGAATGGATC AGCAGTTCCA
    ACATCCTACC AGACAGAGGA GCTTAGACGA GAAGTCATTA TGTTAGCCTG TAGTTTTGGG
    AATAAACAGT GTCACCAGAA AGCAGCAACT ATGATATCTG ACTGGATCTC CAGCAACAGA
    AACCGAATTC CTCCAAATGT CCGTGACATA GTCTATTGCA CCGGAGTATC CTTGATGGAT
    GAAGATGTAT GGGAATTCAT TTGGATGAAA TTTCATTCTT CCACAGCTGT ATCAGAAAAG
    AAAATTCTAT TAGAAGCTCT GACCTGCAGT GATAATAGAA ACCTCCTGAA TAGGCTTTTG
    AATCTCTCCT TGAATTCAGA AGTAGTACTG GATCAAGATG CCATTGATGT CATTATTCAT
    GTGGCAAGAA ACCCACATGG AAGAGATTTA GCTTGGAAAT TCTTCAGAGA CAAATGGAAG
    ATTTTAAATG CTAGGTATGG AGAAGCATTG TTTATGAATT CAAAGCTCAT TAGTGGAGTA
    ACTGAATTTC TTAATACAGA AGAAGAACTA AATGAGCTTA AAGATTTCAT ACAACAGCAC
    AAAGGAGGAT CGGCTGTGGC TTCTCTGTCT CGTGCTACGG AGACTGTCGA TGCAAATGTA
    CGTTGGCAAC ATCTATACAG AGATGAACTC TTTCAGTGGC TGAGAAAAAC AGTGAAACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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