TRHDE cDNA ORF clone, Canis lupus familiaris(Dog)

  • Gene

  • Clones

  • gRNAs

The following TRHDE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRHDE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe291731 XM_022424884.1
Latest version!
Canis lupus familiaris thyrotropin releasing hormone degrading enzyme (TRHDE), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe38758 XM_014117121.1
Latest version!
Canis lupus familiaris thyrotropin-releasing hormone degrading enzyme (TRHDE), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe291731
    Clone ID Related Accession (Same CDS sequence) XM_022424884.1
    Accession Version XM_022424884.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product thyrotropin-releasing hormone-degrading ectoenzyme
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTCGCGC GGGGGGTGCT CCCGGGCGAG GGATGCTCGC GGGGGGGATT CTCTGGGGGG 
    GATGCTCGCG GGGAGGATGC TGTTGGAGCT GCCTGGGCGG CCGTACCCGG AAGCACGGAG
    CGCCCCTCGG CGGAGCACGG GCGGCTGGTG CTAGCCTTCT CCGTGTGCAT CGTGACGCTG
    CTGGCGGTCA CCGCGCTCGC CGTGCTGCTC AGCCTGCGCT TCGACGGGTG CGGGCCGGGC
    GCCCCCGGTT GTGCCCCCGG CCTCCCTGCG CGCGGCGGCA ACGCCAGCTT CCCGGGGCCC
    GCGCGGCGCG ACCCGCAGGC GGGCGGGGAG GCCAGCACCC CCGGGGCCCC GGCCGCGCGG
    CCGCCGTCGG ACGCGGAGCG GGAGCGGGAG CGGGAGCGGG AGCGGGAGCG GGGGCCCGAG
    CGCGAGCCCG AGCGCGAGCA CCCCTGGACG CACCTGCGCC TGCCGGGCCA CCTCAAGCCG
    CTGCACTACA ACCTGATGCT CACCGCCTTC ATGGACAACT TCACCTTCTC CGGGGAGGTC
    AACGTGGAGA TCGCGTGCAC CAACCGCACC CGCTACGTCG TGCTGCACGC CTCCCGCGTG
    GCCGTGGACA AGGTGCAGCT GGCCGAGGAC CGCGCGGCCG GGGCCGTGCC CGTGGCCGGC
    TTCTTCCTCT ACCCGCAGAC CCAGGTCCTG GTGGTGGTGC TCAACCGCAG CCTGGACGCG
    CACCGCAACT ACAACCTGAA GGTCGTCTAC AGCGCGCTCA TCGAGAACGA GCTGCTGGGC
    TTCTTCCGCA GCTCCTACGT GCTCCACGGG GAGAGGAGAT TCCTCGGTGT TACGCAGTTT
    TCACCCACAC ATGCCAGAAA GGCATTTCCT TGTTTTGACG AGCCAATCTA CAAGGCCACT
    TTCAAAATTA GCATCAAGCA TCAAGCAACC TATTTATCTT TATCGAATAT GCCAGTGGAA
    ACTTCCGTGT TTGAGGAAGA TGGATGGGTT ACGGATCACT TTTCACAGAC CCCTCTCATG
    TCTACATATT ATTTAGCCTG GGCGATTTGC AACTTCACAT ACAGAGAAAC TACCACCAAG
    AGTGGGGTTG CAGTACGATT ATATGCAAGA CCCGATGCTA TCAGAAGAGG ATCTGGGGAC
    TATGCTCTCC ATATAACAAA GAGATTAATA GAATTTTATG AAGACTACTT TAAGGTGCCC
    TATTCGTTGC CAAAACTAGA TCTTTTAGCT GTGCCTAAGC ATCCGTACGC TGCTATGGAG
    AACTGGGGAC TAAGTATTTT TGTGGAGCAA AGAATACTGC TGGATCCCAG TGTTTCATCT
    ATTTCTTATT TACTGGATGT CACCATGGTC ATTGTTCATG AGATATGTCA CCAGTGGTTT
    GGTGACCTCG TGACCCCTGT ATGGTGGGAA GACGTGTGGC TAAAGGAAGG TTTTGCTCAC
    TACTTTGAAT TTGTTGGCAC GGACTATCTC TACCCTGGCT GGAACATGGA AAAACAGAGG
    TTTCTGACGG ATGTCCTGCA TGAAGTAATG CTGCTGGACG GCTTGGCCAG TTCCCATCCA
    GTATCACAGG AGGTGCTACA GGCAACAGAT ATTGACAGGG TGTTTGACTG GATCGCCTAT
    AAAAAGGGTG CTGCTCTAAT TAGAATGCTG GCTAATTTTA TGGGCCATTC AGTATTTCAA
    AGGGGTTTAC AGGATTATTT AACCATTCAT AAGTATGGCA ATGCAGCCAG AAACGATCTC
    TGGAATACAC TGTCAGAGGC TTTAAAAAGG AATGGGAAAT TTGTAAATAT ACAAGAAGTG
    ATGGATCAGT GGACACTCCA GATGGGTTAT CCTGTAATCA CCATCTTGGG GAATTTGACG
    GCGGAAAATA GAATAATAAT TACCCAACAA CATTTTATCT ATGACATCAG TGCTAAAACT
    AAAGCACTCC AACTTCAAAA TAACAGTTAC CTGTGGCAGA TTCCATTAAC TATAGTGGTA
    GGAAATAGAA GCCATGTGTC TTCAGAAGCA ATTATTTGGG TGTCTAACAA ATCAGAACAC
    CACAGGATAA CTTTGGACAA AGGAAGCTGG TTGCTGGGGA ACATCAATCA AACTGGCTAT
    TTTAGAGTCA ACTATGATTT AAGGAATTGG AGATTATTAA TTGATCAATT AATCAGGAAC
    CATCAGGTTC TCTCTGTCAG TAACAGAGCG GGTTTGATCG ATGATGCCTT CAGCCTAGCC
    AGGGCCGGCT ATTTGCCTCA GCATATCCCC CTGGAGATTA TCAGATACCT GTCCGAGGAG
    AAGGATTTTC TTCCGTGGCA CGCTGCCAGC CGAGCTCTTT ACCCCCTTGA TAAATTACTG
    GACCGCATGG AGAAATACAA CGTCTTCAAT GAATATATTT TAAAGCAAGT CGCAACAACG
    TACATCAAGC TGGGATGGCC AAAAAACAAC TTTAATGGAT CTCTTGTTCA AGCATCCTAC
    CAACATGAAG AACTACGTAG AGAAGTTATA ATGCTGGCGT GCAGTTTTGG CAACAAGCAC
    TGTCACCAGC AGGCATCAAC ACTTATTTCA GATTGGATTT CCAGCAACAG GAACAGAATA
    CCGCTCAATG TTAGAGACAT AGTCTACTGT ACGGGAGTTT CGCTACTAGA TGAGGATGTC
    TGGGAATTCA TATGGATGAA ATTCCACTCC ACTACGGCAG TTTCTGAGAA GAAAATACTA
    TTGGAAGCCT TAACTTGCAG CGATGACAGG AACCTATTAA ATAGGCTTTT GAATCTCTCA
    CTGAATTCCG AGGTGGTGTT AGATCAAGAT GCAATTGATG TGATAATCCA TGTCGCTCGA
    AATCCACACG GCCGAGACCT TGCCTGGAAG TTTTTCAGAG ATAAATGGAA GATATTAAAT
    ACTAGGTACG GAGAGGCATT ATTTATGAAT TCCAAACTCA TTAGTGGAGT CACAGAGTTT
    CTTAATACTG AAGGTGAACT CAAAGAGTTG AAGAACTTCA TGAAGACCTA CGATGGAGTG
    GCTGCTGCCT CGTTCTTGAG AGCTGTGGAA ACGGTGGAGG CCAACGTGCG CTGGAAAGCG
    CTGTATCAGG ATGAACTCTT CCAGTGGTTA GGAAAAGCTC TGAGACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280592.1
    CDS1..3111
    Translation

    Target ORF information:

    RefSeq Version XM_022424884.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris thyrotropin releasing hormone degrading enzyme (TRHDE), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTCGCGC GGGGGGTGCT CCCGGGCGAG GGATGCTCGC GGGGGGGATT CTCTGGGGGG 
    GATGCTCGCG GGGAGGATGC TGTTGGAGCT GCCTGGGCGG CCGTACCCGG AAGCACGGAG
    CGCCCCTCGG CGGAGCACGG GCGGCTGGTG CTAGCCTTCT CCGTGTGCAT CGTGACGCTG
    CTGGCGGTCA CCGCGCTCGC CGTGCTGCTC AGCCTGCGCT TCGACGGGTG CGGGCCGGGC
    GCCCCCGGTT GTGCCCCCGG CCTCCCTGCG CGCGGCGGCA ACGCCAGCTT CCCGGGGCCC
    GCGCGGCGCG ACCCGCAGGC GGGCGGGGAG GCCAGCACCC CCGGGGCCCC GGCCGCGCGG
    CCGCCGTCGG ACGCGGAGCG GGAGCGGGAG CGGGAGCGGG AGCGGGAGCG GGGGCCCGAG
    CGCGAGCCCG AGCGCGAGCA CCCCTGGACG CACCTGCGCC TGCCGGGCCA CCTCAAGCCG
    CTGCACTACA ACCTGATGCT CACCGCCTTC ATGGACAACT TCACCTTCTC CGGGGAGGTC
    AACGTGGAGA TCGCGTGCAC CAACCGCACC CGCTACGTCG TGCTGCACGC CTCCCGCGTG
    GCCGTGGACA AGGTGCAGCT GGCCGAGGAC CGCGCGGCCG GGGCCGTGCC CGTGGCCGGC
    TTCTTCCTCT ACCCGCAGAC CCAGGTCCTG GTGGTGGTGC TCAACCGCAG CCTGGACGCG
    CACCGCAACT ACAACCTGAA GGTCGTCTAC AGCGCGCTCA TCGAGAACGA GCTGCTGGGC
    TTCTTCCGCA GCTCCTACGT GCTCCACGGG GAGAGGAGAT TCCTCGGTGT TACGCAGTTT
    TCACCCACAC ATGCCAGAAA GGCATTTCCT TGTTTTGACG AGCCAATCTA CAAGGCCACT
    TTCAAAATTA GCATCAAGCA TCAAGCAACC TATTTATCTT TATCGAATAT GCCAGTGGAA
    ACTTCCGTGT TTGAGGAAGA TGGATGGGTT ACGGATCACT TTTCACAGAC CCCTCTCATG
    TCTACATATT ATTTAGCCTG GGCGATTTGC AACTTCACAT ACAGAGAAAC TACCACCAAG
    AGTGGGGTTG CAGTACGATT ATATGCAAGA CCCGATGCTA TCAGAAGAGG ATCTGGGGAC
    TATGCTCTCC ATATAACAAA GAGATTAATA GAATTTTATG AAGACTACTT TAAGGTGCCC
    TATTCGTTGC CAAAACTAGA TCTTTTAGCT GTGCCTAAGC ATCCGTACGC TGCTATGGAG
    AACTGGGGAC TAAGTATTTT TGTGGAGCAA AGAATACTGC TGGATCCCAG TGTTTCATCT
    ATTTCTTATT TACTGGATGT CACCATGGTC ATTGTTCATG AGATATGTCA CCAGTGGTTT
    GGTGACCTCG TGACCCCTGT ATGGTGGGAA GACGTGTGGC TAAAGGAAGG TTTTGCTCAC
    TACTTTGAAT TTGTTGGCAC GGACTATCTC TACCCTGGCT GGAACATGGA AAAACAGAGG
    TTTCTGACGG ATGTCCTGCA TGAAGTAATG CTGCTGGACG GCTTGGCCAG TTCCCATCCA
    GTATCACAGG AGGTGCTACA GGCAACAGAT ATTGACAGGG TGTTTGACTG GATCGCCTAT
    AAAAAGGGTG CTGCTCTAAT TAGAATGCTG GCTAATTTTA TGGGCCATTC AGTATTTCAA
    AGGGGTTTAC AGGATTATTT AACCATTCAT AAGTATGGCA ATGCAGCCAG AAACGATCTC
    TGGAATACAC TGTCAGAGGC TTTAAAAAGG AATGGGAAAT TTGTAAATAT ACAAGAAGTG
    ATGGATCAGT GGACACTCCA GATGGGTTAT CCTGTAATCA CCATCTTGGG GAATTTGACG
    GCGGAAAATA GAATAATAAT TACCCAACAA CATTTTATCT ATGACATCAG TGCTAAAACT
    AAAGCACTCC AACTTCAAAA TAACAGTTAC CTGTGGCAGA TTCCATTAAC TATAGTGGTA
    GGAAATAGAA GCCATGTGTC TTCAGAAGCA ATTATTTGGG TGTCTAACAA ATCAGAACAC
    CACAGGATAA CTTTGGACAA AGGAAGCTGG TTGCTGGGGA ACATCAATCA AACTGGCTAT
    TTTAGAGTCA ACTATGATTT AAGGAATTGG AGATTATTAA TTGATCAATT AATCAGGAAC
    CATCAGGTTC TCTCTGTCAG TAACAGAGCG GGTTTGATCG ATGATGCCTT CAGCCTAGCC
    AGGGCCGGCT ATTTGCCTCA GCATATCCCC CTGGAGATTA TCAGATACCT GTCCGAGGAG
    AAGGATTTTC TTCCGTGGCA CGCTGCCAGC CGAGCTCTTT ACCCCCTTGA TAAATTACTG
    GACCGCATGG AGAAATACAA CGTCTTCAAT GAATATATTT TAAAGCAAGT CGCAACAACG
    TACATCAAGC TGGGATGGCC AAAAAACAAC TTTAATGGAT CTCTTGTTCA AGCATCCTAC
    CAACATGAAG AACTACGTAG AGAAGTTATA ATGCTGGCGT GCAGTTTTGG CAACAAGCAC
    TGTCACCAGC AGGCATCAAC ACTTATTTCA GATTGGATTT CCAGCAACAG GAACAGAATA
    CCGCTCAATG TTAGAGACAT AGTCTACTGT ACGGGAGTTT CGCTACTAGA TGAGGATGTC
    TGGGAATTCA TATGGATGAA ATTCCACTCC ACTACGGCAG TTTCTGAGAA GAAAATACTA
    TTGGAAGCCT TAACTTGCAG CGATGACAGG AACCTATTAA ATAGGCTTTT GAATCTCTCA
    CTGAATTCCG AGGTGGTGTT AGATCAAGAT GCAATTGATG TGATAATCCA TGTCGCTCGA
    AATCCACACG GCCGAGACCT TGCCTGGAAG TTTTTCAGAG ATAAATGGAA GATATTAAAT
    ACTAGGTACG GAGAGGCATT ATTTATGAAT TCCAAACTCA TTAGTGGAGT CACAGAGTTT
    CTTAATACTG AAGGTGAACT CAAAGAGTTG AAGAACTTCA TGAAGACCTA CGATGGAGTG
    GCTGCTGCCT CGTTCTTGAG AGCTGTGGAA ACGGTGGAGG CCAACGTGCG CTGGAAAGCG
    CTGTATCAGG ATGAACTCTT CCAGTGGTTA GGAAAAGCTC TGAGACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38758
    Clone ID Related Accession (Same CDS sequence) XM_014117121.1
    Accession Version XM_014117121.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product thyrotropin-releasing hormone-degrading ectoenzyme
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726077.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGATTCAAA CGGACCTTTG GAATGTACGG CATTGGGAAT ACATCCCATG TGGGGATAAA 
    CGGAAAAGAG CTTCCTTGAG CAGGGCTGTG TTTTCAGTGG TTGTCAGGCT GCAGTGCATG
    TGTATCCCAT CTCTTTTGTT CGGTCATACT CGTAAAGCCA AACACAATAT TTTAGTTGTA
    TATTCTCAGG GCTACAACGC GAGCACGGAG CGCCCCTCGG CGGAGCACGG GCGGCTGGTG
    CTAGCCTTCT CCGTGTGCAT CGTGACGCTG CTGGCGGTCA CCGCGCTCGC CGTGCTGCTC
    AGCCTGCGCT TCGACGGGTG CGGGCCGGGC GCCCCCGGTT GTGCCCCCGG CCTCCCTGCG
    CGCGGCGGCA ACGCCAGCTT CCCGGGGCCC GCGCGGCGCG ACCCGCAGGC GGGCGGGGAG
    GCCAGCACCC CCGGGGCCCC GGCCGCGCGG CCGCCGTCGG ACGCGGAGCG GGAGCGGGAG
    CGGGAGCGGG AGCGGGAGCG GGGGCCCGAG CGCGAGCCCG AGCGCGAGCA CCCCTGGACG
    CACCTGCGCC TGCCGGGCCA CCTCAAGCCG CTGCACTACA ACCTGATGCT CACCGCCTTC
    ATGGACAACT TCACCTTCTC CGGGGAGGTC AACGTGGAGA TCGCGTGCAC CAACCGCACC
    CGCTACGTCG TGCTGCACGC CTCCCGCGTG GCCGTGGACA AGGTGCAGCT GGCCGAGGAC
    CGCGCGGCCG GGGCCGTGCC CGTGGCCGGC TTCTTCCTCT ACCCGCAGAC CCAGGTCCTG
    GTGGTGGTGC TCAACCGCAG CCTGGACGCG CACCGCAACT ACAACCTGAA GGTCGTCTAC
    AGCGCGCTCA TCGAGAACGA GCTGCTGGGC TTCTTCCGCA GCTCCTACGT GCTCCACGGG
    GAGAGGAGAT TCCTCGGTGT TACGCAGTTT TCACCCACAC ATGCCAGAAA GGCATTTCCT
    TGTTTTGACG AGCCAATCTA CAAGGCCACT TTCAAAATTA GCATCAAGCA TCAAGCAACC
    TATTTATCTT TATCGAATAT GCCAGTGGAA ACTTCCGTGT TTGAGGAAGA TGGATGGGTT
    ACGGATCACT TTTCACAGAC CCCTCTCATG TCTACATATT ATTTAGCCTG GGCGATTTGC
    AACTTCACAT ACAGAGAAAC TACCACCAAG AGTGGGGTTG CAGTACGATT ATATGCAAGA
    CCCGATGCTA TCAGAAGAGG ATCTGGGGAC TATGCTCTCC ATATAACAAA GAGATTAATA
    GAATTTTATG AAGACTACTT TAAGGTGCCC TATTCGTTGC CAAAACTAGA TCTTTTAGCT
    GTGCCTAAGC ATCCGTACGC TGCTATGGAG AACTGGGGAC TAAGTATTTT TGTGGAGCAA
    AGAATACTGC TGGATCCCAG TGTTTCATCT ATTTCTTATT TACTGGATGT CACCATGGTC
    ATTGTTCATG AGATATGTCA CCAGTGGTTT GGTGACCTCG TGACCCCTGT ATGGTGGGAA
    GACGTGTGGC TAAAGGAAGG TTTTGCTCAC TACTTTGAAT TTGTTGGCAC GGACTATCTC
    TACCCTGGCT GGAACATGGA AAAACAGAGG TTTCTGACGG ATGTCCTGCA TGAAGTAATG
    CTGCTGGACG GCTTGGCCAG TTCCCATCCA GTATCACAGG AGGTGCTACA GGCAACAGAT
    ATTGACAGGG TGTTTGACTG GATCGCCTAT AAAAAGGGTG CTGCTCTAAT TAGAATGCTG
    GCTAATTTTA TGGGCCATTC AGTATTTCAA AGGGGTTTAC AGGATTATTT AACCATTCAT
    AAGTATGGCA ATGCAGCCAG AAACGATCTC TGGAATACAC TGTCAGAGGC TTTAAAAAGG
    AATGGGAAAT TTGTAAATAT ACAAGAAGTG ATGGATCAGT GGACACTCCA GATGGGTTAT
    CCTGTAATCA CCATCTTGGG GAATTTGACG GCGGAAAATA GAATAATAAT TACCCAACAA
    CATTTTATCT ATGACATCAG TGCTAAAACT AAAGCACTCC AACTTCAAAA TAACAGTTAC
    CTGTGGCAGA TTCCATTAAC TATAGTGGTA GGAAATAGAA GCCATGTGTC TTCAGAAGCA
    ATTATTTGGG TGTCTAACAA ATCAGAACAC CACAGGATAA CTTTGGACAA AGGAAGCTGG
    TTGCTGGGGA ACATCAATCA AACTGGCTAT TTTAGAGTCA ACTATGATTT AAGGAATTGG
    AGATTATTAA TTGATCAATT AATCAGGAAC CATCAGGTTC TCTCTGTCAG TAACAGAGCG
    GGTTTGATCG ATGATGCCTT CAGCCTAGCC AGGGCCGGCT ATTTGCCTCA GCATATCCCC
    CTGGAGATTA TCAGATACCT GTCCGAGGAG AAGGATTTTC TTCCGTGGCA CGCTGCCAGC
    CGAGCTCTTT ACCCCCTTGA TAAATTACTG GACCGCATGG AGAAATACAA CGTCTTCAAT
    GAATATATTT TAAAGCAAGT CGCAACAACG TACATCAAGC TGGGATGGCC AAAAAACAAC
    TTTAATGGAT CTCTTGTTCA AGCATCCTAC CAACATGAAG AACTACGTAG AGAAGTTATA
    ATGCTGGCGT GCAGTTTTGG CAACAAGCAC TGTCACCAGC AGGCATCAAC ACTTATTTCA
    GATTGGATTT CCAGCAACAG GAACAGAATA CCGCTCAATG TTAGAGACAT AGTCTACTGT
    ACGGGAGTTT CGCTACTAGA TGAGGATGTC TGGGAATTCA TATGGATGAA ATTCCACTCC
    ACTACGGCAG TTTCTGAGAA GAAAATACTA TTGGAAGCCT TAACTTGCAG CGATGACAGG
    AACCTATTAA ATAGGCTTTT GAATCTCTCA CTGAATTCCG AGGTGGTGTT AGATCAAGAT
    GCAATTGATG TGATAATCCA TGTCGCTCGA AATCCACACG GCCGAGACCT TGCCTGGAAG
    TTTTTCAGAG ATAAATGGAA GATATTAAAT ACTAGGTACG GAGAGGCATT ATTTATGAAT
    TCCAAACTCA TTAGTGGAGT CACAGAGTTT CTTAATACTG AAGGTGAACT CAAAGAGTTG
    AAGAACTTCA TGAAGACCTA CGATGGAGTG GCTGCTGCCT CGTTCTTGAG AGCTGTGGAA
    ACGGTGGAGG CCAACGTGCG CTGGAAAGCG CTGTATCAGG ATGAACTCTT CCAGTGGTTA
    GGAAAAGCTC TGAGACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972596.1
    CDS1..3201
    Translation

    Target ORF information:

    RefSeq Version XM_014117121.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris thyrotropin-releasing hormone degrading enzyme (TRHDE), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117121.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGATTCAAA CGGACCTTTG GAATGTACGG CATTGGGAAT ACATCCCATG TGGGGATAAA 
    CGGAAAAGAG CTTCCTTGAG CAGGGCTGTG TTTTCAGTGG TTGTCAGGCT GCAGTGCATG
    TGTATCCCAT CTCTTTTGTT CGGTCATACT CGTAAAGCCA AACACAATAT TTTAGTTGTA
    TATTCTCAGG GCTACAACGC GAGCACGGAG CGCCCCTCGG CGGAGCACGG GCGGCTGGTG
    CTAGCCTTCT CCGTGTGCAT CGTGACGCTG CTGGCGGTCA CCGCGCTCGC CGTGCTGCTC
    AGCCTGCGCT TCGACGGGTG CGGGCCGGGC GCCCCCGGTT GTGCCCCCGG CCTCCCTGCG
    CGCGGCGGCA ACGCCAGCTT CCCGGGGCCC GCGCGGCGCG ACCCGCAGGC GGGCGGGGAG
    GCCAGCACCC CCGGGGCCCC GGCCGCGCGG CCGCCGTCGG ACGCGGAGCG GGAGCGGGAG
    CGGGAGCGGG AGCGGGAGCG GGGGCCCGAG CGCGAGCCCG AGCGCGAGCA CCCCTGGACG
    CACCTGCGCC TGCCGGGCCA CCTCAAGCCG CTGCACTACA ACCTGATGCT CACCGCCTTC
    ATGGACAACT TCACCTTCTC CGGGGAGGTC AACGTGGAGA TCGCGTGCAC CAACCGCACC
    CGCTACGTCG TGCTGCACGC CTCCCGCGTG GCCGTGGACA AGGTGCAGCT GGCCGAGGAC
    CGCGCGGCCG GGGCCGTGCC CGTGGCCGGC TTCTTCCTCT ACCCGCAGAC CCAGGTCCTG
    GTGGTGGTGC TCAACCGCAG CCTGGACGCG CACCGCAACT ACAACCTGAA GGTCGTCTAC
    AGCGCGCTCA TCGAGAACGA GCTGCTGGGC TTCTTCCGCA GCTCCTACGT GCTCCACGGG
    GAGAGGAGAT TCCTCGGTGT TACGCAGTTT TCACCCACAC ATGCCAGAAA GGCATTTCCT
    TGTTTTGACG AGCCAATCTA CAAGGCCACT TTCAAAATTA GCATCAAGCA TCAAGCAACC
    TATTTATCTT TATCGAATAT GCCAGTGGAA ACTTCCGTGT TTGAGGAAGA TGGATGGGTT
    ACGGATCACT TTTCACAGAC CCCTCTCATG TCTACATATT ATTTAGCCTG GGCGATTTGC
    AACTTCACAT ACAGAGAAAC TACCACCAAG AGTGGGGTTG CAGTACGATT ATATGCAAGA
    CCCGATGCTA TCAGAAGAGG ATCTGGGGAC TATGCTCTCC ATATAACAAA GAGATTAATA
    GAATTTTATG AAGACTACTT TAAGGTGCCC TATTCGTTGC CAAAACTAGA TCTTTTAGCT
    GTGCCTAAGC ATCCGTACGC TGCTATGGAG AACTGGGGAC TAAGTATTTT TGTGGAGCAA
    AGAATACTGC TGGATCCCAG TGTTTCATCT ATTTCTTATT TACTGGATGT CACCATGGTC
    ATTGTTCATG AGATATGTCA CCAGTGGTTT GGTGACCTCG TGACCCCTGT ATGGTGGGAA
    GACGTGTGGC TAAAGGAAGG TTTTGCTCAC TACTTTGAAT TTGTTGGCAC GGACTATCTC
    TACCCTGGCT GGAACATGGA AAAACAGAGG TTTCTGACGG ATGTCCTGCA TGAAGTAATG
    CTGCTGGACG GCTTGGCCAG TTCCCATCCA GTATCACAGG AGGTGCTACA GGCAACAGAT
    ATTGACAGGG TGTTTGACTG GATCGCCTAT AAAAAGGGTG CTGCTCTAAT TAGAATGCTG
    GCTAATTTTA TGGGCCATTC AGTATTTCAA AGGGGTTTAC AGGATTATTT AACCATTCAT
    AAGTATGGCA ATGCAGCCAG AAACGATCTC TGGAATACAC TGTCAGAGGC TTTAAAAAGG
    AATGGGAAAT TTGTAAATAT ACAAGAAGTG ATGGATCAGT GGACACTCCA GATGGGTTAT
    CCTGTAATCA CCATCTTGGG GAATTTGACG GCGGAAAATA GAATAATAAT TACCCAACAA
    CATTTTATCT ATGACATCAG TGCTAAAACT AAAGCACTCC AACTTCAAAA TAACAGTTAC
    CTGTGGCAGA TTCCATTAAC TATAGTGGTA GGAAATAGAA GCCATGTGTC TTCAGAAGCA
    ATTATTTGGG TGTCTAACAA ATCAGAACAC CACAGGATAA CTTTGGACAA AGGAAGCTGG
    TTGCTGGGGA ACATCAATCA AACTGGCTAT TTTAGAGTCA ACTATGATTT AAGGAATTGG
    AGATTATTAA TTGATCAATT AATCAGGAAC CATCAGGTTC TCTCTGTCAG TAACAGAGCG
    GGTTTGATCG ATGATGCCTT CAGCCTAGCC AGGGCCGGCT ATTTGCCTCA GCATATCCCC
    CTGGAGATTA TCAGATACCT GTCCGAGGAG AAGGATTTTC TTCCGTGGCA CGCTGCCAGC
    CGAGCTCTTT ACCCCCTTGA TAAATTACTG GACCGCATGG AGAAATACAA CGTCTTCAAT
    GAATATATTT TAAAGCAAGT CGCAACAACG TACATCAAGC TGGGATGGCC AAAAAACAAC
    TTTAATGGAT CTCTTGTTCA AGCATCCTAC CAACATGAAG AACTACGTAG AGAAGTTATA
    ATGCTGGCGT GCAGTTTTGG CAACAAGCAC TGTCACCAGC AGGCATCAAC ACTTATTTCA
    GATTGGATTT CCAGCAACAG GAACAGAATA CCGCTCAATG TTAGAGACAT AGTCTACTGT
    ACGGGAGTTT CGCTACTAGA TGAGGATGTC TGGGAATTCA TATGGATGAA ATTCCACTCC
    ACTACGGCAG TTTCTGAGAA GAAAATACTA TTGGAAGCCT TAACTTGCAG CGATGACAGG
    AACCTATTAA ATAGGCTTTT GAATCTCTCA CTGAATTCCG AGGTGGTGTT AGATCAAGAT
    GCAATTGATG TGATAATCCA TGTCGCTCGA AATCCACACG GCCGAGACCT TGCCTGGAAG
    TTTTTCAGAG ATAAATGGAA GATATTAAAT ACTAGGTACG GAGAGGCATT ATTTATGAAT
    TCCAAACTCA TTAGTGGAGT CACAGAGTTT CTTAATACTG AAGGTGAACT CAAAGAGTTG
    AAGAACTTCA TGAAGACCTA CGATGGAGTG GCTGCTGCCT CGTTCTTGAG AGCTGTGGAA
    ACGGTGGAGG CCAACGTGCG CTGGAAAGCG CTGTATCAGG ATGAACTCTT CCAGTGGTTA
    GGAAAAGCTC TGAGACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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