acan cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	acan
Entrez Gene ID	100491091
Full Name	aggrecan
Synonyms	agc1,agcan,cspg1,cspgcp,msk16,sedk,xagc1
General protein information	Preferred Names aggrecan Names aggrecan core protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018092666.1	XP_017948155.1	aggrecan core protein
XM_018092666.2	XP_017948155.2	aggrecan core protein isoform X1
XM_031899229.1	XP_031755089.1	aggrecan core protein isoform X2
XM_031899230.1	XP_031755090.1	aggrecan core protein isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	acana	XP_005166542.1
Danio rerio (zebrafish)	acanb	XP_688042.4
Homo sapiens (human)	ACAN	NP_001126.3
Xenopus tropicalis (tropical clawed frog)	acan	XP_002932297.2

Gene Ontology

The following acan gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the acan cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa55190	XM_018092666.2 Latest version!	Xenopus tropicalis aggrecan (acan), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55191	XM_031899229.1 Latest version!	Xenopus tropicalis aggrecan (acan), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa55192	XM_031899230.1 Latest version!	Xenopus tropicalis aggrecan (acan), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa43554	XM_018092666.1 Latest version!	Xenopus tropicalis aggrecan (acan), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

acan ( XM_018092666.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948155.2 Xenopus tropicalis aggrecan (acan), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa55190
Clone ID Related Accession (Same CDS sequence)	XM_018092666.2
Accession Version	XM_018092666.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	aggrecan core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092666.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT TACTATTAGT GTTCGTTTGC TTGCGGGTCA TCGCTGCAGC GATATCCGTA 
GAAGTTTCAG ACCATCACAA CGCACTGAGC GTGATTATAC CTGTAGAAGC TCCACTGAAA 
GTCCAGCTTG GACATTCCCT TACCATCCCT TGTTTCTTCA TAAATGCTTT GGAAGATGTG 
ACTGTGGCTC CCTCAACAGC ACCCTTAACA CCAAGGATCA AGTGGAGCAA ACATGTCAAA 
GGAAAGGAAA CCGTCCTGCT GGTAGCCACA GATGGACATG TTCGTGTAAA TGACATCTAC 
CAAGGCCGTG TCTCTATGCC CACCTACCCA CTTGCCCCTA CAGATGTCAG CCTGGAAATT 
TCCAACATAC TGTCTAATGA TTCAGGAATC TACCGCTGTG AAGTGATGCA TGGGATAGAA 
GACAGCCAAG CTACAGTAGA GGTTAATGTG AAAGGTGTCG TTTTCCACTA CAGAGCAATC 
TCGACCAGAT ATACTTTGGA CTTTGAAAAT GCCAAAAAAG CCTGCATACA AAACAGTGCC 
ACCATTGCCA CCCCCGAGCA GCTCCAGGCA GCATATGATG ATGGTTACCA CCAATGTGAT 
GCTGGGTGGC TTTCCGACCA GACCGTTCGT TATCCAATTC ATAATCCACG TGAAGCCTGC 
TACGGAGACA AGGACATGTT TCCAGGAGTG AGAACATATG GAGTTCGCGA AACAGATGAA 
ACATATGATG TGTATTGCTA TGTTGAGGAT ATGCAGGGTG AAGTGTTTTA TGCAACATCT 
CCTGAAAAGT TCACCTTTGA TGAAGCAGAA AAGCAATGTA AGAGCCTTGG TGCCAGATTG 
GCCACAACTG GAGAGCTCTA TGTGGCATGG AAAAGTGGAA TGGATATGTG CAGTGCGGGT 
TGGTTGGGAG ACCGCAGTGT TAGGTACCCC ATATCCTTTG CTCGGCCAAA CTGTGGTGGA 
AATTTAGTAG GCGTAAGAAC AGTTTATCTC CACCCCACCA ACCAGACTGG ATATCCTAAT 
CCACTATCTC GGTATGATGC TATCTGCTAC AAAGCTGGAC CAGATGAGGA AATCACTATT 
CAAACCATTA CACAGCCAAG TTTAGAGATC ATCCTTCCCC GTAATGAGAC AGAAGAGGAG 
GCTCGTGGCA GTATTGCTTC TCTTGAACCA ACAGACTTTA CCCTTACACC TACTGATTTT 
GAGGAATTTT TCACAGCAAC CCCTGAGCTT GTGCTAACAG AAGGGCCATT TGAAAATGTC 
ACAGAAGAAG TATTTCCCCT TGAAAATATT ACAGCATCAC TCCAAACAGA AGCCCCTGGT 
ACAGCAGATG TGACATTCTC TGAGGGTCCA GAGTTTAATG CAACAACATC TTTAGGAGAG 
CAAATAGTTC CTGCAACAGC TGCTCCTGCT GAAGCTGGGC CAGAAAAAGA CATTTCTAAG 
ACTGGTGTTG TCTTTCATTA CCGAGCTGCT TCCAGTCGAT ATTCCCTTAA CTTCACCGAG 
GCTAAACAGG CATGTCTGGA CAACAATGCT ATCATTGCAA GCCCTGCTCA GCTCCAGGCT 
GCCTATGAAG CTGGATTTGA TCAATGTGAT GCTGGATGGA TATCAGACCA AACTGTCAGA 
TACCCAATAG TCACTCCAAG AGAAAACTGT CATGGAGACA AGAATGGATT TCCTGGGGTT 
AGAAATTATG GAGTGGTAAA CCCAAATGCA CATTTTGATG TGTACTGCTA CATTGATAAA 
CTTCATGGAG AGGTGTTTTT TGCCACTCAA CCAGACCAGT TTACTTTCCA AGAAGCAAAA 
GAATTTTGTG ATGGACAAAA TGCCTCATTG GCCTCCACTG GACAGCTCTA TATGGCCTGG 
AGGTTGGGGT TGGACAAGTG TAGAGCAGGC TGGTTGTCAG ATGGAAGTGT CCGCTACCCC 
GTTGTGACAC CTCGCAGAGC CTGTGGAGGA GACGTACCTG GAGTGCGGAC AGTCTACATT 
CATCCTAATC AAACAGGATT CCCTGATCCT CTGTCAAAAT ACCACGCCTT TTGTTTCCGA 
GAAGAGATTC CAGGTTTTCC ATCAATTGAG GAGGAGCTTA TTGTGCCTGA GGTAGTCCCA 
GAAGTAGAGA AATTACCTTC TGGAGAAGGA CCAACTTCCG AAATTGAGAC TGTGACTGAA 
TTTGAAAATC AGACAGATAT GTGGCTGGCA AATGTCACTG TAATTCCTAC TATTGATTCA 
TCAGTAACAG TCAGTCCTTC AGTTGTTCCA ATTGTGAGCC CAGATCCAGA AGATATAAGT 
GGAGAGACAT CAGCAATAGG CAGTGGAGTA AGTGGAGAAC TATCTGGATT GCCATCTGGA 
GTATCTAGTG GAGATGTGTC TGGTGCTGAT CTAAGTGGAC AACCATCAGG AGCTGAAGAA 
AGTGGATTTC CATCTGGCAC TGACATTTCT GGAGTGAGTG GACTGCCATC TGGTGACATA 
TCTGGTGACA TATCAGGTGA ACCTGATATT AGTGGACTGC CATCTGGATT TCCTTCAGGA 
GTTGAAGACA TATCAGGTCA AGGTTCTGGA GTTCCAGATA TAAGTGGGTT GTCTTCTGTA 
CCAATTGAAA CAAGTGGTCT CCCTTCTGGA GAGCCATCTG GAGATGAGAT AATTAGTGGT 
TCAGCTTCTG GAATTGAGAT AATTAGTGGC CTTTTTTCTG GTACAGAAGA TTTCAGTGGC 
ATTGCCTCAG GCTTCCCAAC AATTACACAT GTAGATACAA CTTGGGAAGA AGCTGTTACA 
AAACCAACAG AAACTGAACT AGAAGGAAAG GGAGAAATTG AATTAAGTGC ATCAGGAATA 
GAATTAAGTG GAGATCTTTC TGGCTTAGAG CCCAGTGGAG AGGTCTCTGG ATTTGACCTT 
AGTGGGGTTA CTTCATCAGT AGTAGAAAGC AGTGGAGATC TCTCTGGAAT TATTTCTAGT 
GGTGATATTA GTGGAGAGCC ATCAGGTATA ATTGATTTGA GTGGTGAAGT GTCAGGAGTA 
ACTGATATTA GTGGAGATTT GTCAGGATTA AAGGATTTTA GTGGTGAGAC ATCAGGAATA 
TTGGATTTTA GTGGTGAGCT ATCTGGCCTA AGTGGAGAAT CATCAGGAAT TATTTCTGTA 
AGTGGTGGAT TTTCTGGAGA TATAGATTTT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGA TTTCTGGAGT AAGAGAGATT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGC TTTCTGGAGT TCCTGACATC AGTGGCTCGG CCTCCGGTAT CTTAGAGATA 
CCATCAGGTG TTTTTCTTAT TGACCAAGCT TTGACAGAAC TCTCTCAGCC ATCAACAAAA 
GAAGAGGAAG GAAAAGGGTC TGTTGGAATA AGTGGCTTTT ATTCAGGAGA TGTTGACAGC 
TCTGGAGACA TATCTGGCAT CTTGGATGTC AGTGGTGGAC CTTCAGGTTT TATAGACATT 
AGTGGTTTTG TTTCAGGGGC TGTTCCTAGT GGTGATATAG ATATTAGTGG TGTATCAGAC 
ATAAGTGGTG CAACATCTGG AGTTGAATAT GTTAGTGGCT TACAATCTGG ATTTGATGAT 
CTAAGTGGAG TCCCTACTGT TTCTCATATT GATTTCACAT TAAAAGAGCC ACCAACTCAA 
GGTCCTGATA CTCAAGAAGC AGGAGAAGGT CCATCAGTAA TACTTGAGAT CAGTGGAATA 
TCTGGAGAAT CTTTGCCATC AGGAACTCAT GAAGGCAGTC TAGAAAGTTC AGGCATATAT 
ACATCAGGAT TTTCTCAAGT CACACTTGTT ACTGATGATA CTGTAGAAGC CATCACAAAA 
CCAGCAGTTT CACAAGAGCT AGGTGGTGTT ACTTTTGCAT ACCCAATAGA AACATCAGCA 
TCTGGTGATA TTAGCACCGA GGCATCTGCA ACTCATGAAA CCAGTGGAAA ACAAGAAACA 
TCTCTAGAAG GAAGCACTGA ACCAAGCTCT GGACAGCTGA CATTTTTGCC ACCAGAAATA 
TCTGAAACAT CAGAATCTGA AATAACTCAT TCTCCTGAGG AAGCACAACA GGAAACAGAA 
TTAGTTACTT CAGTTGAACT GGGTGAAGTA ACAGATGAAC CCACGATTGA AGCAGCCAGT 
CCTTCAAGTC TAATTGCAAC TGAGTCTGAA GGAATAATTG GATCATGTGC TGATCAACCC 
TGCGGAGCTG GAACATGTAC AGAAAAGGAA GGAAGAATAT ACTGTGCCTG CCTGCCAGGC 
TATGGTGGAG AACTTTGTAA TATAGACATT GATGAGTGCC ACTCAAGCCC CTGTGTGAAT 
GGAGCTACCT GCGTTGATGG CATCGACTCA TTCAAATGCT TATGCCTTCC CAGCTACGGA 
GGGGACCTAT GTCAGATCGA TCTAGAGGTG TGTGAGGATG GATGGACCAA GTTCCAGGGT 
CACTGCTACA AACACTTTTC AGAGAGAGAA ACTTGGGTGG ATGCAGAAAA CGTCTGTCGG 
TATCACCAGT CCCATCTAGC GAGCATTCTC ACACCTGAAG AGCAAGAATT TGTGAATGGT 
AACGCTCAGG ACTATCAATG GATTGGGCTG AATGATAAGA CAATTGAAAA TGATTTCCGC 
TGGTCAGATG CAAACCCCTT GCAATATGAA AACTGGAGGC CAAACCAACC AGATAACTTC 
TTCACCACGG GAGAAGATTG TGTTGTTATG ATCTGGCATG AACGAGGAGA ATGGAATGAT 
GTTCCCTGTA ACTACCACCT GCCATTTACA TGCAAAAAGG GAACAGTGGC TTGTGGTGAT 
CCTCCAGCAG TGCAAAACGC CTACACACTA GGAAAAAAGA AAGCACGTTA TGAAATCAGC 
TCCTTAGTGC GGTATCAATG TAACCAAGGC TACCTACAAC GCCATGTTCC TATAATCCGA 
TGTCTACCAA CTGGGCACTG GGATGAGCCC AGGATACAGT GCATAGATCT TACCACTTAC 
AGACGACACA AGAGAAACCC CAGAAGTCGG TCGAGAGCAA TTGCCACATC TGAACAGGGA 
AAGGCCCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948155.2
CDS	402..5453
Translation	MTTLLLVFVCLRVIAAAISVEVSDHHNALSVIIPVEAPLKVQLGHSLTIPCFFINALEDVTVAPSTAPLTPRIKWSKHVKGKETVLLVATDGHVRVNDIYQGRVSMPTYPLAPTDVSLEISNILSNDSGIYRCEVMHGIEDSQATVEVNVKGVVFHYRAISTRYTLDFENAKKACIQNSATIATPEQLQAAYDDGYHQCDAGWLSDQTVRYPIHNPREACYGDKDMFPGVRTYGVRETDETYDVYCYVEDMQGEVFYATSPEKFTFDEAEKQCKSLGARLATTGELYVAWKSGMDMCSAGWLGDRSVRYPISFARPNCGGNLVGVRTVYLHPTNQTGYPNPLSRYDAICYKAGPDEEITIQTITQPSLEIILPRNETEEEARGSIASLEPTDFTLTPTDFEEFFTATPELVLTEGPFENVTEEVFPLENITASLQTEAPGTADVTFSEGPEFNATTSLGEQIVPATAAPAEAGPEKDISKTGVVFHYRAASSRYSLNFTEAKQACLDNNAIIASPAQLQAAYEAGFDQCDAGWISDQTVRYPIVTPRENCHGDKNGFPGVRNYGVVNPNAHFDVYCYIDKLHGEVFFATQPDQFTFQEAKEFCDGQNASLASTGQLYMAWRLGLDKCRAGWLSDGSVRYPVVTPRRACGGDVPGVRTVYIHPNQTGFPDPLSKYHAFCFREEIPGFPSIEEELIVPEVVPEVEKLPSGEGPTSEIETVTEFENQTDMWLANVTVIPTIDSSVTVSPSVVPIVSPDPEDISGETSAIGSGVSGELSGLPSGVSSGDVSGADLSGQPSGAEESGFPSGTDISGVSGLPSGDISGDISGEPDISGLPSGFPSGVEDISGQGSGVPDISGLSSVPIETSGLPSGEPSGDEIISGSASGIEIISGLFSGTEDFSGIASGFPTITHVDTTWEEAVTKPTETELEGKGEIELSASGIELSGDLSGLEPSGEVSGFDLSGVTSSVVESSGDLSGIISSGDISGEPSGIIDLSGEVSGVTDISGDLSGLKDFSGETSGILDFSGELSGLSGESSGIISVSGGFSGDIDFSGEISGVREISGEISGVREISGEISGVREISGELSGVPDISGSASGILEIPSGVFLIDQALTELSQPSTKEEEGKGSVGISGFYSGDVDSSGDISGILDVSGGPSGFIDISGFVSGAVPSGDIDISGVSDISGATSGVEYVSGLQSGFDDLSGVPTVSHIDFTLKEPPTQGPDTQEAGEGPSVILEISGISGESLPSGTHEGSLESSGIYTSGFSQVTLVTDDTVEAITKPAVSQELGGVTFAYPIETSASGDISTEASATHETSGKQETSLEGSTEPSSGQLTFLPPEISETSESEITHSPEEAQQETELVTSVELGEVTDEPTIEAASPSSLIATESEGIIGSCADQPCGAGTCTEKEGRIYCACLPGYGGELCNIDIDECHSSPCVNGATCVDGIDSFKCLCLPSYGGDLCQIDLEVCEDGWTKFQGHCYKHFSERETWVDAENVCRYHQSHLASILTPEEQEFVNGNAQDYQWIGLNDKTIENDFRWSDANPLQYENWRPNQPDNFFTTGEDCVVMIWHERGEWNDVPCNYHLPFTCKKGTVACGDPPAVQNAYTLGKKKARYEISSLVRYQCNQGYLQRHVPIIRCLPTGHWDEPRIQCIDLTTYRRHKRNPRSRSRAIATSEQGKAH

Target ORF information:

RefSeq Version	XM_018092666.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis aggrecan (acan), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092666.2

ORF Insert Sequence:

ATGACCACTT TACTATTAGT GTTCGTTTGC TTGCGGGTCA TCGCTGCAGC GATATCCGTA 
GAAGTTTCAG ACCATCACAA CGCACTGAGC GTGATTATAC CTGTAGAAGC TCCACTGAAA 
GTCCAGCTTG GACATTCCCT TACCATCCCT TGTTTCTTCA TAAATGCTTT GGAAGATGTG 
ACTGTGGCTC CCTCAACAGC ACCCTTAACA CCAAGGATCA AGTGGAGCAA ACATGTCAAA 
GGAAAGGAAA CCGTCCTGCT GGTAGCCACA GATGGACATG TTCGTGTAAA TGACATCTAC 
CAAGGCCGTG TCTCTATGCC CACCTACCCA CTTGCCCCTA CAGATGTCAG CCTGGAAATT 
TCCAACATAC TGTCTAATGA TTCAGGAATC TACCGCTGTG AAGTGATGCA TGGGATAGAA 
GACAGCCAAG CTACAGTAGA GGTTAATGTG AAAGGTGTCG TTTTCCACTA CAGAGCAATC 
TCGACCAGAT ATACTTTGGA CTTTGAAAAT GCCAAAAAAG CCTGCATACA AAACAGTGCC 
ACCATTGCCA CCCCCGAGCA GCTCCAGGCA GCATATGATG ATGGTTACCA CCAATGTGAT 
GCTGGGTGGC TTTCCGACCA GACCGTTCGT TATCCAATTC ATAATCCACG TGAAGCCTGC 
TACGGAGACA AGGACATGTT TCCAGGAGTG AGAACATATG GAGTTCGCGA AACAGATGAA 
ACATATGATG TGTATTGCTA TGTTGAGGAT ATGCAGGGTG AAGTGTTTTA TGCAACATCT 
CCTGAAAAGT TCACCTTTGA TGAAGCAGAA AAGCAATGTA AGAGCCTTGG TGCCAGATTG 
GCCACAACTG GAGAGCTCTA TGTGGCATGG AAAAGTGGAA TGGATATGTG CAGTGCGGGT 
TGGTTGGGAG ACCGCAGTGT TAGGTACCCC ATATCCTTTG CTCGGCCAAA CTGTGGTGGA 
AATTTAGTAG GCGTAAGAAC AGTTTATCTC CACCCCACCA ACCAGACTGG ATATCCTAAT 
CCACTATCTC GGTATGATGC TATCTGCTAC AAAGCTGGAC CAGATGAGGA AATCACTATT 
CAAACCATTA CACAGCCAAG TTTAGAGATC ATCCTTCCCC GTAATGAGAC AGAAGAGGAG 
GCTCGTGGCA GTATTGCTTC TCTTGAACCA ACAGACTTTA CCCTTACACC TACTGATTTT 
GAGGAATTTT TCACAGCAAC CCCTGAGCTT GTGCTAACAG AAGGGCCATT TGAAAATGTC 
ACAGAAGAAG TATTTCCCCT TGAAAATATT ACAGCATCAC TCCAAACAGA AGCCCCTGGT 
ACAGCAGATG TGACATTCTC TGAGGGTCCA GAGTTTAATG CAACAACATC TTTAGGAGAG 
CAAATAGTTC CTGCAACAGC TGCTCCTGCT GAAGCTGGGC CAGAAAAAGA CATTTCTAAG 
ACTGGTGTTG TCTTTCATTA CCGAGCTGCT TCCAGTCGAT ATTCCCTTAA CTTCACCGAG 
GCTAAACAGG CATGTCTGGA CAACAATGCT ATCATTGCAA GCCCTGCTCA GCTCCAGGCT 
GCCTATGAAG CTGGATTTGA TCAATGTGAT GCTGGATGGA TATCAGACCA AACTGTCAGA 
TACCCAATAG TCACTCCAAG AGAAAACTGT CATGGAGACA AGAATGGATT TCCTGGGGTT 
AGAAATTATG GAGTGGTAAA CCCAAATGCA CATTTTGATG TGTACTGCTA CATTGATAAA 
CTTCATGGAG AGGTGTTTTT TGCCACTCAA CCAGACCAGT TTACTTTCCA AGAAGCAAAA 
GAATTTTGTG ATGGACAAAA TGCCTCATTG GCCTCCACTG GACAGCTCTA TATGGCCTGG 
AGGTTGGGGT TGGACAAGTG TAGAGCAGGC TGGTTGTCAG ATGGAAGTGT CCGCTACCCC 
GTTGTGACAC CTCGCAGAGC CTGTGGAGGA GACGTACCTG GAGTGCGGAC AGTCTACATT 
CATCCTAATC AAACAGGATT CCCTGATCCT CTGTCAAAAT ACCACGCCTT TTGTTTCCGA 
GAAGAGATTC CAGGTTTTCC ATCAATTGAG GAGGAGCTTA TTGTGCCTGA GGTAGTCCCA 
GAAGTAGAGA AATTACCTTC TGGAGAAGGA CCAACTTCCG AAATTGAGAC TGTGACTGAA 
TTTGAAAATC AGACAGATAT GTGGCTGGCA AATGTCACTG TAATTCCTAC TATTGATTCA 
TCAGTAACAG TCAGTCCTTC AGTTGTTCCA ATTGTGAGCC CAGATCCAGA AGATATAAGT 
GGAGAGACAT CAGCAATAGG CAGTGGAGTA AGTGGAGAAC TATCTGGATT GCCATCTGGA 
GTATCTAGTG GAGATGTGTC TGGTGCTGAT CTAAGTGGAC AACCATCAGG AGCTGAAGAA 
AGTGGATTTC CATCTGGCAC TGACATTTCT GGAGTGAGTG GACTGCCATC TGGTGACATA 
TCTGGTGACA TATCAGGTGA ACCTGATATT AGTGGACTGC CATCTGGATT TCCTTCAGGA 
GTTGAAGACA TATCAGGTCA AGGTTCTGGA GTTCCAGATA TAAGTGGGTT GTCTTCTGTA 
CCAATTGAAA CAAGTGGTCT CCCTTCTGGA GAGCCATCTG GAGATGAGAT AATTAGTGGT 
TCAGCTTCTG GAATTGAGAT AATTAGTGGC CTTTTTTCTG GTACAGAAGA TTTCAGTGGC 
ATTGCCTCAG GCTTCCCAAC AATTACACAT GTAGATACAA CTTGGGAAGA AGCTGTTACA 
AAACCAACAG AAACTGAACT AGAAGGAAAG GGAGAAATTG AATTAAGTGC ATCAGGAATA 
GAATTAAGTG GAGATCTTTC TGGCTTAGAG CCCAGTGGAG AGGTCTCTGG ATTTGACCTT 
AGTGGGGTTA CTTCATCAGT AGTAGAAAGC AGTGGAGATC TCTCTGGAAT TATTTCTAGT 
GGTGATATTA GTGGAGAGCC ATCAGGTATA ATTGATTTGA GTGGTGAAGT GTCAGGAGTA 
ACTGATATTA GTGGAGATTT GTCAGGATTA AAGGATTTTA GTGGTGAGAC ATCAGGAATA 
TTGGATTTTA GTGGTGAGCT ATCTGGCCTA AGTGGAGAAT CATCAGGAAT TATTTCTGTA 
AGTGGTGGAT TTTCTGGAGA TATAGATTTT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGA TTTCTGGAGT AAGAGAGATT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGC TTTCTGGAGT TCCTGACATC AGTGGCTCGG CCTCCGGTAT CTTAGAGATA 
CCATCAGGTG TTTTTCTTAT TGACCAAGCT TTGACAGAAC TCTCTCAGCC ATCAACAAAA 
GAAGAGGAAG GAAAAGGGTC TGTTGGAATA AGTGGCTTTT ATTCAGGAGA TGTTGACAGC 
TCTGGAGACA TATCTGGCAT CTTGGATGTC AGTGGTGGAC CTTCAGGTTT TATAGACATT 
AGTGGTTTTG TTTCAGGGGC TGTTCCTAGT GGTGATATAG ATATTAGTGG TGTATCAGAC 
ATAAGTGGTG CAACATCTGG AGTTGAATAT GTTAGTGGCT TACAATCTGG ATTTGATGAT 
CTAAGTGGAG TCCCTACTGT TTCTCATATT GATTTCACAT TAAAAGAGCC ACCAACTCAA 
GGTCCTGATA CTCAAGAAGC AGGAGAAGGT CCATCAGTAA TACTTGAGAT CAGTGGAATA 
TCTGGAGAAT CTTTGCCATC AGGAACTCAT GAAGGCAGTC TAGAAAGTTC AGGCATATAT 
ACATCAGGAT TTTCTCAAGT CACACTTGTT ACTGATGATA CTGTAGAAGC CATCACAAAA 
CCAGCAGTTT CACAAGAGCT AGGTGGTGTT ACTTTTGCAT ACCCAATAGA AACATCAGCA 
TCTGGTGATA TTAGCACCGA GGCATCTGCA ACTCATGAAA CCAGTGGAAA ACAAGAAACA 
TCTCTAGAAG GAAGCACTGA ACCAAGCTCT GGACAGCTGA CATTTTTGCC ACCAGAAATA 
TCTGAAACAT CAGAATCTGA AATAACTCAT TCTCCTGAGG AAGCACAACA GGAAACAGAA 
TTAGTTACTT CAGTTGAACT GGGTGAAGTA ACAGATGAAC CCACGATTGA AGCAGCCAGT 
CCTTCAAGTC TAATTGCAAC TGAGTCTGAA GGAATAATTG GATCATGTGC TGATCAACCC 
TGCGGAGCTG GAACATGTAC AGAAAAGGAA GGAAGAATAT ACTGTGCCTG CCTGCCAGGC 
TATGGTGGAG AACTTTGTAA TATAGACATT GATGAGTGCC ACTCAAGCCC CTGTGTGAAT 
GGAGCTACCT GCGTTGATGG CATCGACTCA TTCAAATGCT TATGCCTTCC CAGCTACGGA 
GGGGACCTAT GTCAGATCGA TCTAGAGGTG TGTGAGGATG GATGGACCAA GTTCCAGGGT 
CACTGCTACA AACACTTTTC AGAGAGAGAA ACTTGGGTGG ATGCAGAAAA CGTCTGTCGG 
TATCACCAGT CCCATCTAGC GAGCATTCTC ACACCTGAAG AGCAAGAATT TGTGAATGGT 
AACGCTCAGG ACTATCAATG GATTGGGCTG AATGATAAGA CAATTGAAAA TGATTTCCGC 
TGGTCAGATG CAAACCCCTT GCAATATGAA AACTGGAGGC CAAACCAACC AGATAACTTC 
TTCACCACGG GAGAAGATTG TGTTGTTATG ATCTGGCATG AACGAGGAGA ATGGAATGAT 
GTTCCCTGTA ACTACCACCT GCCATTTACA TGCAAAAAGG GAACAGTGGC TTGTGGTGAT 
CCTCCAGCAG TGCAAAACGC CTACACACTA GGAAAAAAGA AAGCACGTTA TGAAATCAGC 
TCCTTAGTGC GGTATCAATG TAACCAAGGC TACCTACAAC GCCATGTTCC TATAATCCGA 
TGTCTACCAA CTGGGCACTG GGATGAGCCC AGGATACAGT GCATAGATCT TACCACTTAC 
AGACGACACA AGAGAAACCC CAGAAGTCGG TCGAGAGCAA TTGCCACATC TGAACAGGGA 
AAGGCCCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acan ( XM_031899229.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755089.1 Xenopus tropicalis aggrecan (acan), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa55191
Clone ID Related Accession (Same CDS sequence)	XM_031899229.1
Accession Version	XM_031899229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	aggrecan core protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT TACTATTAGT GTTCGTTTGC TTGCGGGTCA TCGCTGCAGC GATATCCGTA 
GAAGTTTCAG ACCATCACAA CGCACTGAGC GTGATTATAC CTGTAGAAGC TCCACTGAAA 
GTCCAGCTTG GACATTCCCT TACCATCCCT TGTTTCTTCA TAAATGCTTT GGAAGATGTG 
ACTGTGGCTC CCTCAACAGC ACCCTTAACA CCAAGGATCA AGTGGAGCAA ACATGTCAAA 
GGAAAGGAAA CCGTCCTGCT GGTAGCCACA GATGGACATG TTCGTGTAAA TGACATCTAC 
CAAGGCCGTG TCTCTATGCC CACCTACCCA CTTGCCCCTA CAGATGTCAG CCTGGAAATT 
TCCAACATAC TGTCTAATGA TTCAGGAATC TACCGCTGTG AAGTGATGCA TGGGATAGAA 
GACAGCCAAG CTACAGTAGA GGTTAATGTG AAAGGTGTCG TTTTCCACTA CAGAGCAATC 
TCGACCAGAT ATACTTTGGA CTTTGAAAAT GCCAAAAAAG CCTGCATACA AAACAGTGCC 
ACCATTGCCA CCCCCGAGCA GCTCCAGGCA GCATATGATG ATGGTTACCA CCAATGTGAT 
GCTGGGTGGC TTTCCGACCA GACCGTTCGT TATCCAATTC ATAATCCACG TGAAGCCTGC 
TACGGAGACA AGGACATGTT TCCAGGAGTG AGAACATATG GAGTTCGCGA AACAGATGAA 
ACATATGATG TGTATTGCTA TGTTGAGGAT ATGCAGGGTG AAGTGTTTTA TGCAACATCT 
CCTGAAAAGT TCACCTTTGA TGAAGCAGAA AAGCAATGTA AGAGCCTTGG TGCCAGATTG 
GCCACAACTG GAGAGCTCTA TGTGGCATGG AAAAGTGGAA TGGATATGTG CAGTGCGGGT 
TGGTTGGGAG ACCGCAGTGT TAGGTACCCC ATATCCTTTG CTCGGCCAAA CTGTGGTGGA 
AATTTAGTAG GCGTAAGAAC AGTTTATCTC CACCCCACCA ACCAGACTGG ATATCCTAAT 
CCACTATCTC GGTATGATGC TATCTGCTAC AAAGCTGGAC CAGATGAGGA AATCACTATT 
CAAACCATTA CACAGCCAAG TTTAGAGATC ATCCTTCCCC GTAATGAGAC AGAAGAGGAG 
GCTCGTGGCA GTATTGCTTC TCTTGAACCA ACAGACTTTA CCCTTACACC TACTGATTTT 
GAGGAATTTT TCACAGCAAC CCCTGAGCTT GTGCTAACAG AAGGGCCATT TGAAAATGTC 
ACAGAAGAAG TATTTCCCCT TGAAAATATT ACAGCATCAC TCCAAACAGA AGCCCCTGGT 
ACAGCAGATG TGACATTCTC TGAGGGTCCA GAGTTTAATG CAACAACATC TTTAGGAGAG 
CAAATAGTTC CTGCAACAGC TGCTCCTGCT GAAGCTGGGC CAGAAAAAGA CATTTCTAAG 
ACTGGTGTTG TCTTTCATTA CCGAGCTGCT TCCAGTCGAT ATTCCCTTAA CTTCACCGAG 
GCTAAACAGG CATGTCTGGA CAACAATGCT ATCATTGCAA GCCCTGCTCA GCTCCAGGCT 
GCCTATGAAG CTGGATTTGA TCAATGTGAT GCTGGATGGA TATCAGACCA AACTGTCAGA 
TACCCAATAG TCACTCCAAG AGAAAACTGT CATGGAGACA AGAATGGATT TCCTGGGGTT 
AGAAATTATG GAGTGGTAAA CCCAAATGCA CATTTTGATG TGTACTGCTA CATTGATAAA 
CTTCATGGAG AGGTGTTTTT TGCCACTCAA CCAGACCAGT TTACTTTCCA AGAAGCAAAA 
GAATTTTGTG ATGGACAAAA TGCCTCATTG GCCTCCACTG GACAGCTCTA TATGGCCTGG 
AGGTTGGGGT TGGACAAGTG TAGAGCAGGC TGGTTGTCAG ATGGAAGTGT CCGCTACCCC 
GTTGTGACAC CTCGCAGAGC CTGTGGAGGA GACGTACCTG GAGTGCGGAC AGTCTACATT 
CATCCTAATC AAACAGGATT CCCTGATCCT CTGTCAAAAT ACCACGCCTT TTGTTTCCGA 
GAAGAGATTC CAGGTTTTCC ATCAATTGAG GAGGAGCTTA TTGTGCCTGA GGTAGTCCCA 
GAAGTAGAGA AATTACCTTC TGGAGAAGGA CCAACTTCCG AAATTGAGAC TGTGACTGAA 
TTTGAAAATC AGACAGATAT GTGGCTGGCA AATGTCACTG TAATTCCTAC TATTGATTCA 
TCAGTAACAG TCAGTCCTTC AGTTGTTCCA ATTGTGAGCC CAGATCCAGA AGATATAAGT 
GGAGAGACAT CAGCAATAGG CAGTGGAGTA AGTGGAGAAC TATCTGGATT GCCATCTGGA 
GTATCTAGTG GAGATGTGTC TGGTGCTGAT CTAAGTGGAC AACCATCAGG AGCTGAAGAA 
AGTGGATTTC CATCTGGCAC TGACATTTCT GGAGTGAGTG GACTGCCATC TGGTGACATA 
TCTGGTGACA TATCAGGTGA ACCTGATATT AGTGGACTGC CATCTGGATT TCCTTCAGGA 
GTTGAAGACA TATCAGGTCA AGGTTCTGGA GTTCCAGATA TAAGTGGGTT GTCTTCTGTA 
CCAATTGAAA CAAGTGGTCT CCCTTCTGGA GAGCCATCTG GAGATGAGAT AATTAGTGGT 
TCAGCTTCTG GAATTGAGAT AATTAGTGGC CTTTTTTCTG GTACAGAAGA TTTCAGTGGC 
ATTGCCTCAG GCTTCCCAAC AATTACACAT GTAGATACAA CTTGGGAAGA AGCTGTTACA 
AAACCAACAG AAACTGAACT AGAAGGAAAG GGAGAAATTG AATTAAGTGC ATCAGGAATA 
GAATTAAGTG GAGATCTTTC TGGCTTAGAG CCCAGTGGAG AGGTCTCTGG ATTTGACCTT 
AGTGGGGTTA CTTCATCAGT AGTAGAAAGC AGTGGAGATC TCTCTGGAAT TATTTCTAGT 
GGTGATATTA GTGGAGAGCC ATCAGGTATA ATTGATTTGA GTGGTGAAGT GTCAGGAGTA 
ACTGATATTA GTGGAGATTT GTCAGGATTA AAGGATTTTA GTGGTGAGAC ATCAGGAATA 
TTGGATTTTA GTGGTGAGCT ATCTGGCCTA AGTGGAGAAT CATCAGGAAT TATTTCTGTA 
AGTGGTGGAT TTTCTGGAGA TATAGATTTT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGA TTTCTGGAGT AAGAGAGATT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGC TTTCTGGAGT TCCTGACATC AGTGGCTCGG CCTCCGGTAT CTTAGAGATA 
CCATCAGGTG TTTTTCTTAT TGACCAAGCT TTGACAGAAC TCTCTCAGCC ATCAACAAAA 
GAAGAGGAAG GAAAAGGGTC TGTTGGAATA AGTGGCTTTT ATTCAGGAGA TGTTGACAGC 
TCTGGAGACA TATCTGGCAT CTTGGATGTC AGTGGTGGAC CTTCAGGTTT TATAGACATT 
AGTGGTTTTG TTTCAGGGGC TGTTCCTAGT GGTGATATAG ATATTAGTGG TGTATCAGAC 
ATAAGTGGTG CAACATCTGG AGTTGAATAT GTTAGTGGCT TACAATCTGG ATTTGATGAT 
CTAAGTGGAG TCCCTACTGT TTCTCATATT GATTTCACAT TAAAAGAGCC ACCAACTCAA 
GGTCCTGATA CTCAAGAAGC AGGAGAAGGT CCATCAGTAA TACTTGAGAT CAGTGGAATA 
TCTGGAGAAT CTTTGCCATC AGGAACTCAT GAAGGCAGTC TAGAAAGTTC AGGCATATAT 
ACATCAGGAT TTTCTCAAGT CACACTTGTT ACTGATGATA CTGTAGAAGC CATCACAAAA 
CCAGCAGTTT CACAAGAGCT AGGTGGTGTT ACTTTTGCAT ACCCAATAGA AACATCAGCA 
TCTGGTGATA TTAGCACCGA GGCATCTGCA ACTCATGAAA CCAGTGGAAA ACAAGAAACA 
TCTCTAGAAG GAAGCACTGA ACCAAGCTCT GGACAGCTGA CATTTTTGCC ACCAGAAATA 
TCTGAAACAT CAGAATCTGA AATAACTCAT TCTCCTGAGG AAGCACAACA GGAAACAGAA 
TTAGTTACTT CAGTTGAACT GGGTGAAGTA ACAGATGAAC CCACGATTGA AGCAGCCAGT 
CCTTCAAGTC TAATTGCAAC TGAGTCTGAA GGAATAATTG GATCATGTGC TGATCAACCC 
TGCGGAGCTG GAACATGTAC AGAAAAGGAA GGAAGAATAT ACTGTGCCTG CCTGCCAGGC 
TATGGTGGAG AACTTTGTAA TATAGATCTA GAGGTGTGTG AGGATGGATG GACCAAGTTC 
CAGGGTCACT GCTACAAACA CTTTTCAGAG AGAGAAACTT GGGTGGATGC AGAAAACGTC 
TGTCGGTATC ACCAGTCCCA TCTAGCGAGC ATTCTCACAC CTGAAGAGCA AGAATTTGTG 
AATGGTAACG CTCAGGACTA TCAATGGATT GGGCTGAATG ATAAGACAAT TGAAAATGAT 
TTCCGCTGGT CAGATGCAAA CCCCTTGCAA TATGAAAACT GGAGGCCAAA CCAACCAGAT 
AACTTCTTCA CCACGGGAGA AGATTGTGTT GTTATGATCT GGCATGAACG AGGAGAATGG 
AATGATGTTC CCTGTAACTA CCACCTGCCA TTTACATGCA AAAAGGGAAC AGTGGCTTGT 
GGTGATCCTC CAGCAGTGCA AAACGCCTAC ACACTAGGAA AAAAGAAAGC ACGTTATGAA 
ATCAGCTCCT TAGTGCGGTA TCAATGTAAC CAAGGCTACC TACAACGCCA TGTTCCTATA 
ATCCGATGTC TACCAACTGG GCACTGGGAT GAGCCCAGGA TACAGTGCAT AGATCTTACC 
ACTTACAGAC GACACAAGAG AAACCCCAGA AGTCGGTCGA GAGCAATTGC CACATCTGAA 
CAGGGAAAGG CCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755089.1
CDS	402..5339
Translation	MTTLLLVFVCLRVIAAAISVEVSDHHNALSVIIPVEAPLKVQLGHSLTIPCFFINALEDVTVAPSTAPLTPRIKWSKHVKGKETVLLVATDGHVRVNDIYQGRVSMPTYPLAPTDVSLEISNILSNDSGIYRCEVMHGIEDSQATVEVNVKGVVFHYRAISTRYTLDFENAKKACIQNSATIATPEQLQAAYDDGYHQCDAGWLSDQTVRYPIHNPREACYGDKDMFPGVRTYGVRETDETYDVYCYVEDMQGEVFYATSPEKFTFDEAEKQCKSLGARLATTGELYVAWKSGMDMCSAGWLGDRSVRYPISFARPNCGGNLVGVRTVYLHPTNQTGYPNPLSRYDAICYKAGPDEEITIQTITQPSLEIILPRNETEEEARGSIASLEPTDFTLTPTDFEEFFTATPELVLTEGPFENVTEEVFPLENITASLQTEAPGTADVTFSEGPEFNATTSLGEQIVPATAAPAEAGPEKDISKTGVVFHYRAASSRYSLNFTEAKQACLDNNAIIASPAQLQAAYEAGFDQCDAGWISDQTVRYPIVTPRENCHGDKNGFPGVRNYGVVNPNAHFDVYCYIDKLHGEVFFATQPDQFTFQEAKEFCDGQNASLASTGQLYMAWRLGLDKCRAGWLSDGSVRYPVVTPRRACGGDVPGVRTVYIHPNQTGFPDPLSKYHAFCFREEIPGFPSIEEELIVPEVVPEVEKLPSGEGPTSEIETVTEFENQTDMWLANVTVIPTIDSSVTVSPSVVPIVSPDPEDISGETSAIGSGVSGELSGLPSGVSSGDVSGADLSGQPSGAEESGFPSGTDISGVSGLPSGDISGDISGEPDISGLPSGFPSGVEDISGQGSGVPDISGLSSVPIETSGLPSGEPSGDEIISGSASGIEIISGLFSGTEDFSGIASGFPTITHVDTTWEEAVTKPTETELEGKGEIELSASGIELSGDLSGLEPSGEVSGFDLSGVTSSVVESSGDLSGIISSGDISGEPSGIIDLSGEVSGVTDISGDLSGLKDFSGETSGILDFSGELSGLSGESSGIISVSGGFSGDIDFSGEISGVREISGEISGVREISGEISGVREISGELSGVPDISGSASGILEIPSGVFLIDQALTELSQPSTKEEEGKGSVGISGFYSGDVDSSGDISGILDVSGGPSGFIDISGFVSGAVPSGDIDISGVSDISGATSGVEYVSGLQSGFDDLSGVPTVSHIDFTLKEPPTQGPDTQEAGEGPSVILEISGISGESLPSGTHEGSLESSGIYTSGFSQVTLVTDDTVEAITKPAVSQELGGVTFAYPIETSASGDISTEASATHETSGKQETSLEGSTEPSSGQLTFLPPEISETSESEITHSPEEAQQETELVTSVELGEVTDEPTIEAASPSSLIATESEGIIGSCADQPCGAGTCTEKEGRIYCACLPGYGGELCNIDLEVCEDGWTKFQGHCYKHFSERETWVDAENVCRYHQSHLASILTPEEQEFVNGNAQDYQWIGLNDKTIENDFRWSDANPLQYENWRPNQPDNFFTTGEDCVVMIWHERGEWNDVPCNYHLPFTCKKGTVACGDPPAVQNAYTLGKKKARYEISSLVRYQCNQGYLQRHVPIIRCLPTGHWDEPRIQCIDLTTYRRHKRNPRSRSRAIATSEQGKAH

Target ORF information:

RefSeq Version	XM_031899229.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis aggrecan (acan), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899229.1

ORF Insert Sequence:

ATGACCACTT TACTATTAGT GTTCGTTTGC TTGCGGGTCA TCGCTGCAGC GATATCCGTA 
GAAGTTTCAG ACCATCACAA CGCACTGAGC GTGATTATAC CTGTAGAAGC TCCACTGAAA 
GTCCAGCTTG GACATTCCCT TACCATCCCT TGTTTCTTCA TAAATGCTTT GGAAGATGTG 
ACTGTGGCTC CCTCAACAGC ACCCTTAACA CCAAGGATCA AGTGGAGCAA ACATGTCAAA 
GGAAAGGAAA CCGTCCTGCT GGTAGCCACA GATGGACATG TTCGTGTAAA TGACATCTAC 
CAAGGCCGTG TCTCTATGCC CACCTACCCA CTTGCCCCTA CAGATGTCAG CCTGGAAATT 
TCCAACATAC TGTCTAATGA TTCAGGAATC TACCGCTGTG AAGTGATGCA TGGGATAGAA 
GACAGCCAAG CTACAGTAGA GGTTAATGTG AAAGGTGTCG TTTTCCACTA CAGAGCAATC 
TCGACCAGAT ATACTTTGGA CTTTGAAAAT GCCAAAAAAG CCTGCATACA AAACAGTGCC 
ACCATTGCCA CCCCCGAGCA GCTCCAGGCA GCATATGATG ATGGTTACCA CCAATGTGAT 
GCTGGGTGGC TTTCCGACCA GACCGTTCGT TATCCAATTC ATAATCCACG TGAAGCCTGC 
TACGGAGACA AGGACATGTT TCCAGGAGTG AGAACATATG GAGTTCGCGA AACAGATGAA 
ACATATGATG TGTATTGCTA TGTTGAGGAT ATGCAGGGTG AAGTGTTTTA TGCAACATCT 
CCTGAAAAGT TCACCTTTGA TGAAGCAGAA AAGCAATGTA AGAGCCTTGG TGCCAGATTG 
GCCACAACTG GAGAGCTCTA TGTGGCATGG AAAAGTGGAA TGGATATGTG CAGTGCGGGT 
TGGTTGGGAG ACCGCAGTGT TAGGTACCCC ATATCCTTTG CTCGGCCAAA CTGTGGTGGA 
AATTTAGTAG GCGTAAGAAC AGTTTATCTC CACCCCACCA ACCAGACTGG ATATCCTAAT 
CCACTATCTC GGTATGATGC TATCTGCTAC AAAGCTGGAC CAGATGAGGA AATCACTATT 
CAAACCATTA CACAGCCAAG TTTAGAGATC ATCCTTCCCC GTAATGAGAC AGAAGAGGAG 
GCTCGTGGCA GTATTGCTTC TCTTGAACCA ACAGACTTTA CCCTTACACC TACTGATTTT 
GAGGAATTTT TCACAGCAAC CCCTGAGCTT GTGCTAACAG AAGGGCCATT TGAAAATGTC 
ACAGAAGAAG TATTTCCCCT TGAAAATATT ACAGCATCAC TCCAAACAGA AGCCCCTGGT 
ACAGCAGATG TGACATTCTC TGAGGGTCCA GAGTTTAATG CAACAACATC TTTAGGAGAG 
CAAATAGTTC CTGCAACAGC TGCTCCTGCT GAAGCTGGGC CAGAAAAAGA CATTTCTAAG 
ACTGGTGTTG TCTTTCATTA CCGAGCTGCT TCCAGTCGAT ATTCCCTTAA CTTCACCGAG 
GCTAAACAGG CATGTCTGGA CAACAATGCT ATCATTGCAA GCCCTGCTCA GCTCCAGGCT 
GCCTATGAAG CTGGATTTGA TCAATGTGAT GCTGGATGGA TATCAGACCA AACTGTCAGA 
TACCCAATAG TCACTCCAAG AGAAAACTGT CATGGAGACA AGAATGGATT TCCTGGGGTT 
AGAAATTATG GAGTGGTAAA CCCAAATGCA CATTTTGATG TGTACTGCTA CATTGATAAA 
CTTCATGGAG AGGTGTTTTT TGCCACTCAA CCAGACCAGT TTACTTTCCA AGAAGCAAAA 
GAATTTTGTG ATGGACAAAA TGCCTCATTG GCCTCCACTG GACAGCTCTA TATGGCCTGG 
AGGTTGGGGT TGGACAAGTG TAGAGCAGGC TGGTTGTCAG ATGGAAGTGT CCGCTACCCC 
GTTGTGACAC CTCGCAGAGC CTGTGGAGGA GACGTACCTG GAGTGCGGAC AGTCTACATT 
CATCCTAATC AAACAGGATT CCCTGATCCT CTGTCAAAAT ACCACGCCTT TTGTTTCCGA 
GAAGAGATTC CAGGTTTTCC ATCAATTGAG GAGGAGCTTA TTGTGCCTGA GGTAGTCCCA 
GAAGTAGAGA AATTACCTTC TGGAGAAGGA CCAACTTCCG AAATTGAGAC TGTGACTGAA 
TTTGAAAATC AGACAGATAT GTGGCTGGCA AATGTCACTG TAATTCCTAC TATTGATTCA 
TCAGTAACAG TCAGTCCTTC AGTTGTTCCA ATTGTGAGCC CAGATCCAGA AGATATAAGT 
GGAGAGACAT CAGCAATAGG CAGTGGAGTA AGTGGAGAAC TATCTGGATT GCCATCTGGA 
GTATCTAGTG GAGATGTGTC TGGTGCTGAT CTAAGTGGAC AACCATCAGG AGCTGAAGAA 
AGTGGATTTC CATCTGGCAC TGACATTTCT GGAGTGAGTG GACTGCCATC TGGTGACATA 
TCTGGTGACA TATCAGGTGA ACCTGATATT AGTGGACTGC CATCTGGATT TCCTTCAGGA 
GTTGAAGACA TATCAGGTCA AGGTTCTGGA GTTCCAGATA TAAGTGGGTT GTCTTCTGTA 
CCAATTGAAA CAAGTGGTCT CCCTTCTGGA GAGCCATCTG GAGATGAGAT AATTAGTGGT 
TCAGCTTCTG GAATTGAGAT AATTAGTGGC CTTTTTTCTG GTACAGAAGA TTTCAGTGGC 
ATTGCCTCAG GCTTCCCAAC AATTACACAT GTAGATACAA CTTGGGAAGA AGCTGTTACA 
AAACCAACAG AAACTGAACT AGAAGGAAAG GGAGAAATTG AATTAAGTGC ATCAGGAATA 
GAATTAAGTG GAGATCTTTC TGGCTTAGAG CCCAGTGGAG AGGTCTCTGG ATTTGACCTT 
AGTGGGGTTA CTTCATCAGT AGTAGAAAGC AGTGGAGATC TCTCTGGAAT TATTTCTAGT 
GGTGATATTA GTGGAGAGCC ATCAGGTATA ATTGATTTGA GTGGTGAAGT GTCAGGAGTA 
ACTGATATTA GTGGAGATTT GTCAGGATTA AAGGATTTTA GTGGTGAGAC ATCAGGAATA 
TTGGATTTTA GTGGTGAGCT ATCTGGCCTA AGTGGAGAAT CATCAGGAAT TATTTCTGTA 
AGTGGTGGAT TTTCTGGAGA TATAGATTTT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGA TTTCTGGAGT AAGAGAGATT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGC TTTCTGGAGT TCCTGACATC AGTGGCTCGG CCTCCGGTAT CTTAGAGATA 
CCATCAGGTG TTTTTCTTAT TGACCAAGCT TTGACAGAAC TCTCTCAGCC ATCAACAAAA 
GAAGAGGAAG GAAAAGGGTC TGTTGGAATA AGTGGCTTTT ATTCAGGAGA TGTTGACAGC 
TCTGGAGACA TATCTGGCAT CTTGGATGTC AGTGGTGGAC CTTCAGGTTT TATAGACATT 
AGTGGTTTTG TTTCAGGGGC TGTTCCTAGT GGTGATATAG ATATTAGTGG TGTATCAGAC 
ATAAGTGGTG CAACATCTGG AGTTGAATAT GTTAGTGGCT TACAATCTGG ATTTGATGAT 
CTAAGTGGAG TCCCTACTGT TTCTCATATT GATTTCACAT TAAAAGAGCC ACCAACTCAA 
GGTCCTGATA CTCAAGAAGC AGGAGAAGGT CCATCAGTAA TACTTGAGAT CAGTGGAATA 
TCTGGAGAAT CTTTGCCATC AGGAACTCAT GAAGGCAGTC TAGAAAGTTC AGGCATATAT 
ACATCAGGAT TTTCTCAAGT CACACTTGTT ACTGATGATA CTGTAGAAGC CATCACAAAA 
CCAGCAGTTT CACAAGAGCT AGGTGGTGTT ACTTTTGCAT ACCCAATAGA AACATCAGCA 
TCTGGTGATA TTAGCACCGA GGCATCTGCA ACTCATGAAA CCAGTGGAAA ACAAGAAACA 
TCTCTAGAAG GAAGCACTGA ACCAAGCTCT GGACAGCTGA CATTTTTGCC ACCAGAAATA 
TCTGAAACAT CAGAATCTGA AATAACTCAT TCTCCTGAGG AAGCACAACA GGAAACAGAA 
TTAGTTACTT CAGTTGAACT GGGTGAAGTA ACAGATGAAC CCACGATTGA AGCAGCCAGT 
CCTTCAAGTC TAATTGCAAC TGAGTCTGAA GGAATAATTG GATCATGTGC TGATCAACCC 
TGCGGAGCTG GAACATGTAC AGAAAAGGAA GGAAGAATAT ACTGTGCCTG CCTGCCAGGC 
TATGGTGGAG AACTTTGTAA TATAGATCTA GAGGTGTGTG AGGATGGATG GACCAAGTTC 
CAGGGTCACT GCTACAAACA CTTTTCAGAG AGAGAAACTT GGGTGGATGC AGAAAACGTC 
TGTCGGTATC ACCAGTCCCA TCTAGCGAGC ATTCTCACAC CTGAAGAGCA AGAATTTGTG 
AATGGTAACG CTCAGGACTA TCAATGGATT GGGCTGAATG ATAAGACAAT TGAAAATGAT 
TTCCGCTGGT CAGATGCAAA CCCCTTGCAA TATGAAAACT GGAGGCCAAA CCAACCAGAT 
AACTTCTTCA CCACGGGAGA AGATTGTGTT GTTATGATCT GGCATGAACG AGGAGAATGG 
AATGATGTTC CCTGTAACTA CCACCTGCCA TTTACATGCA AAAAGGGAAC AGTGGCTTGT 
GGTGATCCTC CAGCAGTGCA AAACGCCTAC ACACTAGGAA AAAAGAAAGC ACGTTATGAA 
ATCAGCTCCT TAGTGCGGTA TCAATGTAAC CAAGGCTACC TACAACGCCA TGTTCCTATA 
ATCCGATGTC TACCAACTGG GCACTGGGAT GAGCCCAGGA TACAGTGCAT AGATCTTACC 
ACTTACAGAC GACACAAGAG AAACCCCAGA AGTCGGTCGA GAGCAATTGC CACATCTGAA 
CAGGGAAAGG CCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acan ( XM_031899230.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755090.1 Xenopus tropicalis aggrecan (acan), transcript variant X3, mRNA.

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CloneID	OXa55192
Clone ID Related Accession (Same CDS sequence)	XM_031899230.1
Accession Version	XM_031899230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	aggrecan core protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT TACTATTAGT GTTCGTTTGC TTGCGGGTCA TCGCTGCAGC GATATCCGTA 
GAAGTTTCAG ACCATCACAA CGCACTGAGC GTGATTATAC CTGTAGAAGC TCCACTGAAA 
GTCCAGCTTG GACATTCCCT TACCATCCCT TGTTTCTTCA TAAATGCTTT GGAAGATGTG 
ACTGTGGCTC CCTCAACAGC ACCCTTAACA CCAAGGATCA AGTGGAGCAA ACATGTCAAA 
GGAAAGGAAA CCGTCCTGCT GGTAGCCACA GATGGACATG TTCGTGTAAA TGACATCTAC 
CAAGGCCGTG TCTCTATGCC CACCTACCCA CTTGCCCCTA CAGATGTCAG CCTGGAAATT 
TCCAACATAC TGTCTAATGA TTCAGGAATC TACCGCTGTG AAGTGATGCA TGGGATAGAA 
GACAGCCAAG CTACAGTAGA GGTTAATGTG AAAGGTGTCG TTTTCCACTA CAGAGCAATC 
TCGACCAGAT ATACTTTGGA CTTTGAAAAT GCCAAAAAAG CCTGCATACA AAACAGTGCC 
ACCATTGCCA CCCCCGAGCA GCTCCAGGCA GCATATGATG ATGGTTACCA CCAATGTGAT 
GCTGGGTGGC TTTCCGACCA GACCGTTCGT TATCCAATTC ATAATCCACG TGAAGCCTGC 
TACGGAGACA AGGACATGTT TCCAGGAGTG AGAACATATG GAGTTCGCGA AACAGATGAA 
ACATATGATG TGTATTGCTA TGTTGAGGAT ATGCAGGGTG AAGTGTTTTA TGCAACATCT 
CCTGAAAAGT TCACCTTTGA TGAAGCAGAA AAGCAATGTA AGAGCCTTGG TGCCAGATTG 
GCCACAACTG GAGAGCTCTA TGTGGCATGG AAAAGTGGAA TGGATATGTG CAGTGCGGGT 
TGGTTGGGAG ACCGCAGTGT TAGGTACCCC ATATCCTTTG CTCGGCCAAA CTGTGGTGGA 
AATTTAGTAG GCGTAAGAAC AGTTTATCTC CACCCCACCA ACCAGACTGG ATATCCTAAT 
CCACTATCTC GGTATGATGC TATCTGCTAC AAAGCTGGAC CAGATGAGGA AATCACTATT 
CAAACCATTA CACAGCCAAG TTTAGAGATC ATCCTTCCCC GTAATGAGAC AGAAGAGGAG 
GCTCGTGGCA GTATTGCTTC TCTTGAACCA ACAGACTTTA CCCTTACACC TACTGATTTT 
GAGGAATTTT TCACAGCAAC CCCTGAGCTT GTGCTAACAG AAGGGCCATT TGAAAATGTC 
ACAGAAGAAG TATTTCCCCT TGAAAATATT ACAGCATCAC TCCAAACAGA AGCCCCTGGT 
ACAGCAGATG TGACATTCTC TGAGGGTCCA GAGTTTAATG CAACAACATC TTTAGGAGAG 
CAAATAGTTC CTGCAACAGC TGCTCCTGCT GAAGCTGGGC CAGAAAAAGA CATTTCTAAG 
ACTGGTGTTG TCTTTCATTA CCGAGCTGCT TCCAGTCGAT ATTCCCTTAA CTTCACCGAG 
GCTAAACAGG CATGTCTGGA CAACAATGCT ATCATTGCAA GCCCTGCTCA GCTCCAGGCT 
GCCTATGAAG CTGGATTTGA TCAATGTGAT GCTGGATGGA TATCAGACCA AACTGTCAGA 
TACCCAATAG TCACTCCAAG AGAAAACTGT CATGGAGACA AGAATGGATT TCCTGGGGTT 
AGAAATTATG GAGTGGTAAA CCCAAATGCA CATTTTGATG TGTACTGCTA CATTGATAAA 
CTTCATGGAG AGGTGTTTTT TGCCACTCAA CCAGACCAGT TTACTTTCCA AGAAGCAAAA 
GAATTTTGTG ATGGACAAAA TGCCTCATTG GCCTCCACTG GACAGCTCTA TATGGCCTGG 
AGGTTGGGGT TGGACAAGTG TAGAGCAGGC TGGTTGTCAG ATGGAAGTGT CCGCTACCCC 
GTTGTGACAC CTCGCAGAGC CTGTGGAGGA GACGTACCTG GAGTGCGGAC AGTCTACATT 
CATCCTAATC AAACAGGATT CCCTGATCCT CTGTCAAAAT ACCACGCCTT TTGTTTCCGA 
GAAGAGATTC CAGGTTTTCC ATCAATTGAG GAGGAGCTTA TTGTGCCTGA GGTAGTCCCA 
GAAGTAGAGA AATTACCTTC TGGAGAAGGA CCAACTTCCG AAATTGAGAC TGTGACTGAA 
TTTGAAAATC AGACAGATAT GTGGCTGGCA AATGTCACTG TAATTCCTAC TATTGATTCA 
TCAGTAACAG TCAGTCCTTC AGTTGTTCCA ATTGTGAGCC CAGATCCAGA AGATATAAGT 
GGAGAGACAT CAGCAATAGG CAGTGGAGTA AGTGGAGAAC TATCTGGATT GCCATCTGGA 
GTATCTAGTG GAGATGTGTC TGGTGCTGAT CTAAGTGGAC AACCATCAGG AGCTGAAGAA 
AGTGGATTTC CATCTGGCAC TGACATTTCT GGAGTGAGTG GACTGCCATC TGGTGACATA 
TCTGGTGACA TATCAGGTGA ACCTGATATT AGTGGACTGC CATCTGGATT TCCTTCAGGA 
GTTGAAGACA TATCAGGTCA AGGTTCTGGA GTTCCAGATA TAAGTGGGTT GTCTTCTGTA 
CCAATTGAAA CAAGTGGTCT CCCTTCTGGA GAGCCATCTG GAGATGAGAT AATTAGTGGT 
TCAGCTTCTG GAATTGAGAT AATTAGTGGC CTTTTTTCTG GTACAGAAGA TTTCAGTGGC 
ATTGCCTCAG GCTTCCCAAC AATTACACAT GTAGATACAA CTTGGGAAGA AGCTGTTACA 
AAACCAACAG AAACTGAACT AGAAGGAAAG GGAGAAATTG AATTAAGTGC ATCAGGAATA 
GAATTAAGTG GAGATCTTTC TGGCTTAGAG CCCAGTGGAG AGGTCTCTGG ATTTGACCTT 
AGTGGGGTTA CTTCATCAGT AGTAGAAAGC AGTGGAGATC TCTCTGGAAT TATTTCTAGT 
GGTGATATTA GTGGAGAGCC ATCAGGTATA ATTGATTTGA GTGGTGAAGT GTCAGGAGTA 
ACTGATATTA GTGGAGATTT GTCAGGATTA AAGGATTTTA GTGGTGAGAC ATCAGGAATA 
TTGGATTTTA GTGGTGAGCT ATCTGGCCTA AGTGGAGAAT CATCAGGAAT TATTTCTGTA 
AGTGGTGGAT TTTCTGGAGA TATAGATTTT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGA TTTCTGGAGT AAGAGAGATT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGC TTTCTGGAGT TCCTGACATC AGTGGCTCGG CCTCCGGTAT CTTAGAGATA 
CCATCAGGTG TTTTTCTTAT TGACCAAGCT TTGACAGAAC TCTCTCAGCC ATCAACAAAA 
GAAGAGGAAG GAAAAGGGTC TGTTGGAATA AGTGGCTTTT ATTCAGGAGA TGTTGACAGC 
TCTGGAGACA TATCTGGCAT CTTGGATGTC AGTGGTGGAC CTTCAGGTTT TATAGACATT 
AGTGGTTTTG TTTCAGGGGC TGTTCCTAGT GGTGATATAG ATATTAGTGG TGTATCAGAC 
ATAAGTGGTG CAACATCTGG AGTTGAATAT GTTAGTGGCT TACAATCTGG ATTTGATGAT 
CTAAGTGGAG TCCCTACTGT TTCTCATATT GATTTCACAT TAAAAGAGCC ACCAACTCAA 
GGTCCTGATA CTCAAGAAGC AGGAGAAGGT CCATCAGTAA TACTTGAGAT CAGTGGAATA 
TCTGGAGAAT CTTTGCCATC AGGAACTCAT GAAGGCAGTC TAGAAAGTTC AGGCATATAT 
ACATCAGGAT TTTCTCAAGT CACACTTGTT ACTGATGATA CTGTAGAAGC CATCACAAAA 
CCAGCAGTTT CACAAGAGCT AGGTGGTGTT ACTTTTGCAT ACCCAATAGA AACATCAGCA 
TCTGGTGATA TTAGCACCGA GGCATCTGCA ACTCATGAAA CCAGTGGAAA ACAAGAAACA 
TCTCTAGAAG GAAGCACTGA ACCAAGCTCT GGACAGCTGA CATTTTTGCC ACCAGAAATA 
TCTGAAACAT CAGAATCTGA AATAACTCAT TCTCCTGAGG AAGCACAACA GGAAACAGAA 
TTAGTTACTT CAGTTGAACT GGGTGAAGTA ACAGATGAAC CCACGATTGA AGCAGCCAGT 
CCTTCAAGTC TAATTGCAAC TGAGTCTGAA GATCTAGAGG TGTGTGAGGA TGGATGGACC 
AAGTTCCAGG GTCACTGCTA CAAACACTTT TCAGAGAGAG AAACTTGGGT GGATGCAGAA 
AACGTCTGTC GGTATCACCA GTCCCATCTA GCGAGCATTC TCACACCTGA AGAGCAAGAA 
TTTGTGAATG GTAACGCTCA GGACTATCAA TGGATTGGGC TGAATGATAA GACAATTGAA 
AATGATTTCC GCTGGTCAGA TGCAAACCCC TTGCAATATG AAAACTGGAG GCCAAACCAA 
CCAGATAACT TCTTCACCAC GGGAGAAGAT TGTGTTGTTA TGATCTGGCA TGAACGAGGA 
GAATGGAATG ATGTTCCCTG TAACTACCAC CTGCCATTTA CATGCAAAAA GGGAACAGTG 
GCTTGTGGTG ATCCTCCAGC AGTGCAAAAC GCCTACACAC TAGGAAAAAA GAAAGCACGT 
TATGAAATCA GCTCCTTAGT GCGGTATCAA TGTAACCAAG GCTACCTACA ACGCCATGTT 
CCTATAATCC GATGTCTACC AACTGGGCAC TGGGATGAGC CCAGGATACA GTGCATAGAT 
CTTACCACTT ACAGACGACA CAAGAGAAAC CCCAGAAGTC GGTCGAGAGC AATTGCCACA 
TCTGAACAGG GAAAGGCCCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755090.1
CDS	402..5225
Translation	MTTLLLVFVCLRVIAAAISVEVSDHHNALSVIIPVEAPLKVQLGHSLTIPCFFINALEDVTVAPSTAPLTPRIKWSKHVKGKETVLLVATDGHVRVNDIYQGRVSMPTYPLAPTDVSLEISNILSNDSGIYRCEVMHGIEDSQATVEVNVKGVVFHYRAISTRYTLDFENAKKACIQNSATIATPEQLQAAYDDGYHQCDAGWLSDQTVRYPIHNPREACYGDKDMFPGVRTYGVRETDETYDVYCYVEDMQGEVFYATSPEKFTFDEAEKQCKSLGARLATTGELYVAWKSGMDMCSAGWLGDRSVRYPISFARPNCGGNLVGVRTVYLHPTNQTGYPNPLSRYDAICYKAGPDEEITIQTITQPSLEIILPRNETEEEARGSIASLEPTDFTLTPTDFEEFFTATPELVLTEGPFENVTEEVFPLENITASLQTEAPGTADVTFSEGPEFNATTSLGEQIVPATAAPAEAGPEKDISKTGVVFHYRAASSRYSLNFTEAKQACLDNNAIIASPAQLQAAYEAGFDQCDAGWISDQTVRYPIVTPRENCHGDKNGFPGVRNYGVVNPNAHFDVYCYIDKLHGEVFFATQPDQFTFQEAKEFCDGQNASLASTGQLYMAWRLGLDKCRAGWLSDGSVRYPVVTPRRACGGDVPGVRTVYIHPNQTGFPDPLSKYHAFCFREEIPGFPSIEEELIVPEVVPEVEKLPSGEGPTSEIETVTEFENQTDMWLANVTVIPTIDSSVTVSPSVVPIVSPDPEDISGETSAIGSGVSGELSGLPSGVSSGDVSGADLSGQPSGAEESGFPSGTDISGVSGLPSGDISGDISGEPDISGLPSGFPSGVEDISGQGSGVPDISGLSSVPIETSGLPSGEPSGDEIISGSASGIEIISGLFSGTEDFSGIASGFPTITHVDTTWEEAVTKPTETELEGKGEIELSASGIELSGDLSGLEPSGEVSGFDLSGVTSSVVESSGDLSGIISSGDISGEPSGIIDLSGEVSGVTDISGDLSGLKDFSGETSGILDFSGELSGLSGESSGIISVSGGFSGDIDFSGEISGVREISGEISGVREISGEISGVREISGELSGVPDISGSASGILEIPSGVFLIDQALTELSQPSTKEEEGKGSVGISGFYSGDVDSSGDISGILDVSGGPSGFIDISGFVSGAVPSGDIDISGVSDISGATSGVEYVSGLQSGFDDLSGVPTVSHIDFTLKEPPTQGPDTQEAGEGPSVILEISGISGESLPSGTHEGSLESSGIYTSGFSQVTLVTDDTVEAITKPAVSQELGGVTFAYPIETSASGDISTEASATHETSGKQETSLEGSTEPSSGQLTFLPPEISETSESEITHSPEEAQQETELVTSVELGEVTDEPTIEAASPSSLIATESEDLEVCEDGWTKFQGHCYKHFSERETWVDAENVCRYHQSHLASILTPEEQEFVNGNAQDYQWIGLNDKTIENDFRWSDANPLQYENWRPNQPDNFFTTGEDCVVMIWHERGEWNDVPCNYHLPFTCKKGTVACGDPPAVQNAYTLGKKKARYEISSLVRYQCNQGYLQRHVPIIRCLPTGHWDEPRIQCIDLTTYRRHKRNPRSRSRAIATSEQGKAH

Target ORF information:

RefSeq Version	XM_031899230.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis aggrecan (acan), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899230.1

ORF Insert Sequence:

ATGACCACTT TACTATTAGT GTTCGTTTGC TTGCGGGTCA TCGCTGCAGC GATATCCGTA 
GAAGTTTCAG ACCATCACAA CGCACTGAGC GTGATTATAC CTGTAGAAGC TCCACTGAAA 
GTCCAGCTTG GACATTCCCT TACCATCCCT TGTTTCTTCA TAAATGCTTT GGAAGATGTG 
ACTGTGGCTC CCTCAACAGC ACCCTTAACA CCAAGGATCA AGTGGAGCAA ACATGTCAAA 
GGAAAGGAAA CCGTCCTGCT GGTAGCCACA GATGGACATG TTCGTGTAAA TGACATCTAC 
CAAGGCCGTG TCTCTATGCC CACCTACCCA CTTGCCCCTA CAGATGTCAG CCTGGAAATT 
TCCAACATAC TGTCTAATGA TTCAGGAATC TACCGCTGTG AAGTGATGCA TGGGATAGAA 
GACAGCCAAG CTACAGTAGA GGTTAATGTG AAAGGTGTCG TTTTCCACTA CAGAGCAATC 
TCGACCAGAT ATACTTTGGA CTTTGAAAAT GCCAAAAAAG CCTGCATACA AAACAGTGCC 
ACCATTGCCA CCCCCGAGCA GCTCCAGGCA GCATATGATG ATGGTTACCA CCAATGTGAT 
GCTGGGTGGC TTTCCGACCA GACCGTTCGT TATCCAATTC ATAATCCACG TGAAGCCTGC 
TACGGAGACA AGGACATGTT TCCAGGAGTG AGAACATATG GAGTTCGCGA AACAGATGAA 
ACATATGATG TGTATTGCTA TGTTGAGGAT ATGCAGGGTG AAGTGTTTTA TGCAACATCT 
CCTGAAAAGT TCACCTTTGA TGAAGCAGAA AAGCAATGTA AGAGCCTTGG TGCCAGATTG 
GCCACAACTG GAGAGCTCTA TGTGGCATGG AAAAGTGGAA TGGATATGTG CAGTGCGGGT 
TGGTTGGGAG ACCGCAGTGT TAGGTACCCC ATATCCTTTG CTCGGCCAAA CTGTGGTGGA 
AATTTAGTAG GCGTAAGAAC AGTTTATCTC CACCCCACCA ACCAGACTGG ATATCCTAAT 
CCACTATCTC GGTATGATGC TATCTGCTAC AAAGCTGGAC CAGATGAGGA AATCACTATT 
CAAACCATTA CACAGCCAAG TTTAGAGATC ATCCTTCCCC GTAATGAGAC AGAAGAGGAG 
GCTCGTGGCA GTATTGCTTC TCTTGAACCA ACAGACTTTA CCCTTACACC TACTGATTTT 
GAGGAATTTT TCACAGCAAC CCCTGAGCTT GTGCTAACAG AAGGGCCATT TGAAAATGTC 
ACAGAAGAAG TATTTCCCCT TGAAAATATT ACAGCATCAC TCCAAACAGA AGCCCCTGGT 
ACAGCAGATG TGACATTCTC TGAGGGTCCA GAGTTTAATG CAACAACATC TTTAGGAGAG 
CAAATAGTTC CTGCAACAGC TGCTCCTGCT GAAGCTGGGC CAGAAAAAGA CATTTCTAAG 
ACTGGTGTTG TCTTTCATTA CCGAGCTGCT TCCAGTCGAT ATTCCCTTAA CTTCACCGAG 
GCTAAACAGG CATGTCTGGA CAACAATGCT ATCATTGCAA GCCCTGCTCA GCTCCAGGCT 
GCCTATGAAG CTGGATTTGA TCAATGTGAT GCTGGATGGA TATCAGACCA AACTGTCAGA 
TACCCAATAG TCACTCCAAG AGAAAACTGT CATGGAGACA AGAATGGATT TCCTGGGGTT 
AGAAATTATG GAGTGGTAAA CCCAAATGCA CATTTTGATG TGTACTGCTA CATTGATAAA 
CTTCATGGAG AGGTGTTTTT TGCCACTCAA CCAGACCAGT TTACTTTCCA AGAAGCAAAA 
GAATTTTGTG ATGGACAAAA TGCCTCATTG GCCTCCACTG GACAGCTCTA TATGGCCTGG 
AGGTTGGGGT TGGACAAGTG TAGAGCAGGC TGGTTGTCAG ATGGAAGTGT CCGCTACCCC 
GTTGTGACAC CTCGCAGAGC CTGTGGAGGA GACGTACCTG GAGTGCGGAC AGTCTACATT 
CATCCTAATC AAACAGGATT CCCTGATCCT CTGTCAAAAT ACCACGCCTT TTGTTTCCGA 
GAAGAGATTC CAGGTTTTCC ATCAATTGAG GAGGAGCTTA TTGTGCCTGA GGTAGTCCCA 
GAAGTAGAGA AATTACCTTC TGGAGAAGGA CCAACTTCCG AAATTGAGAC TGTGACTGAA 
TTTGAAAATC AGACAGATAT GTGGCTGGCA AATGTCACTG TAATTCCTAC TATTGATTCA 
TCAGTAACAG TCAGTCCTTC AGTTGTTCCA ATTGTGAGCC CAGATCCAGA AGATATAAGT 
GGAGAGACAT CAGCAATAGG CAGTGGAGTA AGTGGAGAAC TATCTGGATT GCCATCTGGA 
GTATCTAGTG GAGATGTGTC TGGTGCTGAT CTAAGTGGAC AACCATCAGG AGCTGAAGAA 
AGTGGATTTC CATCTGGCAC TGACATTTCT GGAGTGAGTG GACTGCCATC TGGTGACATA 
TCTGGTGACA TATCAGGTGA ACCTGATATT AGTGGACTGC CATCTGGATT TCCTTCAGGA 
GTTGAAGACA TATCAGGTCA AGGTTCTGGA GTTCCAGATA TAAGTGGGTT GTCTTCTGTA 
CCAATTGAAA CAAGTGGTCT CCCTTCTGGA GAGCCATCTG GAGATGAGAT AATTAGTGGT 
TCAGCTTCTG GAATTGAGAT AATTAGTGGC CTTTTTTCTG GTACAGAAGA TTTCAGTGGC 
ATTGCCTCAG GCTTCCCAAC AATTACACAT GTAGATACAA CTTGGGAAGA AGCTGTTACA 
AAACCAACAG AAACTGAACT AGAAGGAAAG GGAGAAATTG AATTAAGTGC ATCAGGAATA 
GAATTAAGTG GAGATCTTTC TGGCTTAGAG CCCAGTGGAG AGGTCTCTGG ATTTGACCTT 
AGTGGGGTTA CTTCATCAGT AGTAGAAAGC AGTGGAGATC TCTCTGGAAT TATTTCTAGT 
GGTGATATTA GTGGAGAGCC ATCAGGTATA ATTGATTTGA GTGGTGAAGT GTCAGGAGTA 
ACTGATATTA GTGGAGATTT GTCAGGATTA AAGGATTTTA GTGGTGAGAC ATCAGGAATA 
TTGGATTTTA GTGGTGAGCT ATCTGGCCTA AGTGGAGAAT CATCAGGAAT TATTTCTGTA 
AGTGGTGGAT TTTCTGGAGA TATAGATTTT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGA TTTCTGGAGT AAGAGAGATT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGC TTTCTGGAGT TCCTGACATC AGTGGCTCGG CCTCCGGTAT CTTAGAGATA 
CCATCAGGTG TTTTTCTTAT TGACCAAGCT TTGACAGAAC TCTCTCAGCC ATCAACAAAA 
GAAGAGGAAG GAAAAGGGTC TGTTGGAATA AGTGGCTTTT ATTCAGGAGA TGTTGACAGC 
TCTGGAGACA TATCTGGCAT CTTGGATGTC AGTGGTGGAC CTTCAGGTTT TATAGACATT 
AGTGGTTTTG TTTCAGGGGC TGTTCCTAGT GGTGATATAG ATATTAGTGG TGTATCAGAC 
ATAAGTGGTG CAACATCTGG AGTTGAATAT GTTAGTGGCT TACAATCTGG ATTTGATGAT 
CTAAGTGGAG TCCCTACTGT TTCTCATATT GATTTCACAT TAAAAGAGCC ACCAACTCAA 
GGTCCTGATA CTCAAGAAGC AGGAGAAGGT CCATCAGTAA TACTTGAGAT CAGTGGAATA 
TCTGGAGAAT CTTTGCCATC AGGAACTCAT GAAGGCAGTC TAGAAAGTTC AGGCATATAT 
ACATCAGGAT TTTCTCAAGT CACACTTGTT ACTGATGATA CTGTAGAAGC CATCACAAAA 
CCAGCAGTTT CACAAGAGCT AGGTGGTGTT ACTTTTGCAT ACCCAATAGA AACATCAGCA 
TCTGGTGATA TTAGCACCGA GGCATCTGCA ACTCATGAAA CCAGTGGAAA ACAAGAAACA 
TCTCTAGAAG GAAGCACTGA ACCAAGCTCT GGACAGCTGA CATTTTTGCC ACCAGAAATA 
TCTGAAACAT CAGAATCTGA AATAACTCAT TCTCCTGAGG AAGCACAACA GGAAACAGAA 
TTAGTTACTT CAGTTGAACT GGGTGAAGTA ACAGATGAAC CCACGATTGA AGCAGCCAGT 
CCTTCAAGTC TAATTGCAAC TGAGTCTGAA GATCTAGAGG TGTGTGAGGA TGGATGGACC 
AAGTTCCAGG GTCACTGCTA CAAACACTTT TCAGAGAGAG AAACTTGGGT GGATGCAGAA 
AACGTCTGTC GGTATCACCA GTCCCATCTA GCGAGCATTC TCACACCTGA AGAGCAAGAA 
TTTGTGAATG GTAACGCTCA GGACTATCAA TGGATTGGGC TGAATGATAA GACAATTGAA 
AATGATTTCC GCTGGTCAGA TGCAAACCCC TTGCAATATG AAAACTGGAG GCCAAACCAA 
CCAGATAACT TCTTCACCAC GGGAGAAGAT TGTGTTGTTA TGATCTGGCA TGAACGAGGA 
GAATGGAATG ATGTTCCCTG TAACTACCAC CTGCCATTTA CATGCAAAAA GGGAACAGTG 
GCTTGTGGTG ATCCTCCAGC AGTGCAAAAC GCCTACACAC TAGGAAAAAA GAAAGCACGT 
TATGAAATCA GCTCCTTAGT GCGGTATCAA TGTAACCAAG GCTACCTACA ACGCCATGTT 
CCTATAATCC GATGTCTACC AACTGGGCAC TGGGATGAGC CCAGGATACA GTGCATAGAT 
CTTACCACTT ACAGACGACA CAAGAGAAAC CCCAGAAGTC GGTCGAGAGC AATTGCCACA 
TCTGAACAGG GAAAGGCCCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acan ( XM_018092666.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948155.1 Xenopus tropicalis aggrecan (acan), mRNA.

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CloneID	OXa43554
Clone ID Related Accession (Same CDS sequence)	XM_018092666.1
Accession Version	XM_018092666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	aggrecan core protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGACCACTT TACTATTAGT GTTCGTTTGC TTGCGGGTCA TCGCTGCAGC GATATCCGTA 
GAAGTTTCAG ACCATCACAA CGCACTGAGT GTGATTATAC CTGTAGAAGC TCCACTGAAA 
GTCCAGCTTG GACATTCCCT TACCATCCCT TGTTTCTTCA TAAATGCTTT GGAAGATGTG 
ACTGTGGCTC CCTCAACAGC ACCCTTAACA CCAAGGATCA AGTGGAGCAA ACATGTCAAA 
GGAAAGGAAA CCGTCCTGCT GGTAGCCACA GATGGACATG TTCGTGTAAA TGACATCTAC 
CAAGGCCGTG TCTCTATGCC CACCTACCCA CTTGCCCCTA CAGATGTCAG CCTGGAAATT 
TCCAACATAC TGTCTAATGA TTCAGGAATC TACCGCTGTG AAGTGATGCA TGGGATAGAA 
GACAGCCAAG CTACAGTAGA GGTTAATGTG AAAGGTGTCG TTTTCCACTA CAGAGCAATC 
TCGACCAGAT ATACTTTGGA CTTTGAAAAT GCCAAAAAAG CCTGCATACA AAACAGTGCC 
ACCATTGCCA CCCCCGAGCA GCTCCAGGCA GCATATGATG ATGGTTACCA CCAATGTGAT 
GCTGGGTGGC TTTCCGACCA GACCGTTCGT TATCCAATTC ATAATCCACG TGAAGCCTGC 
TACGGAGACA AGGACATGTT TCCAGGAGTG AGAACATATG GAGTTCGCGA AACAGATGAA 
ACATATGATG TGTATTGCTA TGTTGAGGAT ATGCAGGGTG AAGTGTTTTA TGCAACATCT 
CCTGAAAAGT TCACCTTTGA TGAAGCAGAA AAGCAATGTA AGAGCCTTGG TGCCAGATTG 
GCCACAACTG GAGAGCTCTA TGTGGCATGG AAAAGTGGAA TGGATATGTG CAGTGCGGGT 
TGGTTGGGAG ACCGCAGTGT TAGGTACCCC ATATCCTTTG CTCGGCCAAA CTGTGGTGGA 
AATTTAGTAG GCGTAAGAAC AGTTTATCTC CACCCCACCA ACCAGACTGG ATATCCTAAT 
CCACTATCTC GGTATGATGC TATCTGCTAC AAAGCTGGAC CAGATGAGGA AATCACTATT 
CAAACCATTA CACAGCCAAG TTTAGAGATC ATCCTTCCCC GTAATGAGAC AGAAGAGGAG 
GCTCGTGGCA GTATTGCTTC TCTTGAACCA ACAGACTTTA CCCTTACACC TACTGATTTT 
GAGGAATTTT TCACAGCAAC CCCTGAGCTT GTGCTAACAG AAGGGCAATT TGAAAATGTC 
ACAGAAGAAG TATTTCCCCT TGAAAATATT ACAGCATCAC TCCAAACAGA AGCCCCTGGT 
ACAGCAGATG TGACATTCTC TGAGGGTCCA GAGTTTAATG CAACAACATC TTTAGGAGAG 
CAAATAGTTC CTGCAACAGC TGCTCCTGCT GAAGCTGGGC CAGAAAAAGA CATTTCTAAG 
ACTGGTGTTG TCTTTCATTA CCGAGCTGCT TCCAGTCGAT ATTCCCTTAA CTTCACCGAG 
GCTAAACAGG CATGTCTGGA CAACAATGCT ATCATTGCAA GCCCTGCTCA GCTCCAGGCT 
GCCTATGAAG CTGGATTTGA TCAATGTGAT GCTGGATGGA TATCAGACCA AACTGTCAGA 
TACCCAATAG TCACTCCAAG AGAAAACTGT CATGGAGACA AGAATGGATT TCCTGGGGTT 
AGAAATTATG GAGTGGTAAA CCCAAATGCA CATTTTGATG TGTACTGCTA CATTGATAAA 
CTTCATGGAG AGGTGTTTTT TGCCACTCAA CCAGACCAGT TTACTTTCCA AGAAGCAAAA 
GAATTTTGTG ATGGACAAAA TGCCTCATTG GCCTCCACTG GACAGCTCTA TATGGCCTGG 
AGGTTGGGGT TGGACAAGTG TAGAGCAGGC TGGTTGTCAG ATGGAAGTGT CCGCTACCCC 
GTTGTGACAC CTCGCAGAGC CTGTGGAGGA GACGTACCTG GAGTGCGGAC AGTCTACATT 
CATCCTAATC AAACAGGATT CCCTGATCCT CTGTCAAAAT ACCACGCCTT TTGTTTCCGA 
GAAGAGATTC CAGGTTTTCC ATCAATTGAG GAGGAGCTTA TTGTGCCTGA GGTAGTCCCA 
GAAGTAGAGA AATTACCTTC TGGAGAAGGA CCAACTTCCG AAATTGAGAC TGTGACTGAA 
TTTGAAAATC AGACAGATAT GTGGCTGGCA AATGTCACTG TAATTCCTAC TATTGATTCA 
TCAGTAACAG TCAGTCCTTC AGTTGTTCCA ATTGTGAGCC CAGATCCAGA AGATATAAGT 
GGAGAGACAT CAGCAATAGG CAGTGGAGTA AGTGGAGAAC TATCTGGATT GCCATCTGGA 
GTATCTAGTG GAGATGTGTC TGGTGCTGAT CTAAGTGGAC AACCATCAGG AGCTGAAGAA 
AGTGGATTTC CATCTGGCAC TGACATTTCT GGAGTGAGTG GACTGCCATC TGGTGACATA 
TCTGGTGACA TATCAGGTGA ACCTGATATT AGTGGACTGC CATCTGGATT TCCTTCAGGA 
GTTGAAGACA TATCAGGTCA AGGTTCTGGA GTTCCAGATA TAAGTGGGTT GTCTTCTGTA 
CCAATTGAAA CAAGTGGTCT CCCTTCTGGA GAGCCATCTG GAGATGAGAT AATTAGTGGT 
TCAGCTTCTG GAATTGAGAT AATTAGTGGC CTTTTTTCTG GTACAGAAGA TTTCAGTGGC 
ATTGCCTCAG GCTTCCCAAC AATTACACAT GTAGATACAA CTTGGGAAGA AGCTGTTACA 
AAACCAACAG AAACTGAACT AGAAGGAAAG GGAGAAATTG AATTAAGTGC ATCAGGAATA 
GAATTAAGTG GAGATCTTTC TGGCTTAGAG CCCAGTGGAG AGGTCTCTGG ATTTGACCTT 
AGTGGGGTTA CTTCATCAGT AGTAGAAAGC AGTGGAGATC TCTCTGGAAT TATTTCTAGT 
GGTGATATTA GTGGAGAGCC ATCAGGTATA ATTGATTTGA GTGGTGAAGT GTCAGGAGTA 
ACTGATATTA GTGGAGATTT GTCAGGATTA AAGGATTTTA GTGGTGAGAC ATCAGGAATA 
TTGGATTTTA GTGGTGAGCT ATCTGGCCTA AGTGGAGAAT CATCAGGAAT TATTTCTGTA 
AGTGGTGGAT TTTCTGGAGA TATAGATTTT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGA TTTCTGGAGT AAGAGAGATT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGC TTTCTGGAGT TCCTGACATC AGTGGCTCGG CCTCCGGTAT CTTAGAGATA 
CCATCAGGTG TTTTTCTTAT TGACCAAGCT TTGACAGAAC TCTCTCAGCC ATCAACAAAA 
GAAGAGGAAG GAAAAGGGTC TGTTGGAATA AGTGGCTTTT ATTCAGGAGA TGTTGACAGC 
TCTGGAGACA TATCTGGCAT CTTGGATGTC AGTGGTGGAC CTTCAGGTTT TATAGACATT 
AGTGGTTTTG TTTCAGGGGC TGTTCCTAGT GGTGATATAG ATATTAGTGG TGTATCAGAC 
ATAAGTGGTG CAACATCTGG AGTTGAATAT GTTAGTGGCT TACAATCTGG ATTTGATGAT 
CTAAGTGGAG TCCCTACTGT TTCTCATATT GATTTCACAT TAAAAGAGCC ACCAACTCAA 
GGTCCTGATA CTCAAGAAGC AGGAGAAGGT CCATCAGTAA TACTTGAGAT CAGTGGAATA 
TCTGGAGAAT CTTTGCCATC AGGAACTCAT GAAGGCAGTC TAGAAAGTTC AGGCATATAT 
ACATCAGGAT TTTCTCAAGT CACACTTGTT ACTGATGATA CTGTAGAAGC CATCACAAAA 
CCAGCAGTTT CACAAGAGCT AGGTGGTGTT ACTTTTGCAT ACCCAATAGA AACATCAGCA 
TCTGGTGATA TTAGCACCGA GGCATCTGCA ACTCATGAAA CCAGTGGAAA ACAAGAAACA 
TCTCTAGAAG GAAGCACTGA ACCAAGCTCT GGACAGCTGA CATTTTTGCC ACCAGAAATA 
TCTGAAACAT CAGAATCTGA AATAACTCAT TCTCCTGAGG AAGCACAACA GGAAACAGAA 
TTAGTTACTT CAGTTGAACT GGGTGAAGTA ACAGATGAAC CCACGATTGA AGCAGCCAGT 
CCTTCAAGTC TAATTGCAAC TGAGTCTGAA GGAATAATTG GATCATGTGC TGATCAACCC 
TGCGGAGCTG GAACATGTAC AGAAAAGGAA GGAAGAATAT ACTGTGCCTG CCTGCCAGGC 
TATGGTGGAG AACTTTGTAA TATAGACATT GATGAGTGCC ACTCAAGCCC CTGTGTGAAT 
GGAGCTACCT GCGTTGATGG CATCGACTCA TTCAAATGCT TATGCCTTCC CAGCTACGGA 
GGGGACCTAT GTCAGATCGA TCTAGAGGTG TGTGAGGATG GATGGACCAA GTTCCAGGGT 
CACTGCTACA AACACTTTTC AGAGAGAGAA ACTTGGGTGG ATGCAGAAAA CGTCTGTCGG 
TATCACCAGT CCCATCTAGC GAGCATTCTC ACACCTGAAG AGCAAGAATT TGTGAATGGT 
AACGCTCAGG ACTATCAATG GATTGGGCTG AATGATAAGA CAATTGAAAA TGATTTCCGC 
TGGTCAGATG CAAACCCCTT GCAATATGAA AACTGGAGGC CAAACCAACC AGATAACTTC 
TTCACCACGG GAGAAGATTG TGTTGTTATG ATCTGGCATG AACGTGGAGA ATGGAATGAT 
GTTCCCTGTA ACTACCACCT GCCATTTACA TGCAAAAAGG GAACAGTGGC TTGTGGTGAT 
CCTCCAGCAG TGCAAAATGC CTACACACTA GGAAAAAAGA AAGCACGTTA TGAAATCAGC 
TCCTTAGTGC GGTATCAATG TAACCAAGGC TACCTACAAC GTCATGTTCC TATAATCCGA 
TGTCTACCAA CTGGGCACTG GGATGAGCCC AGGATACAGT GCATAGATCT TACCACTTAC 
AGACGACACA AGAGAAACCC CAGAAGTCGG TCGAGAGCAA TTGCCACATC TGAACAGGGA 
AAGGCCCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948155.1
CDS	1..5052
Translation	MTTLLLVFVCLRVIAAAISVEVSDHHNALSVIIPVEAPLKVQLGHSLTIPCFFINALEDVTVAPSTAPLTPRIKWSKHVKGKETVLLVATDGHVRVNDIYQGRVSMPTYPLAPTDVSLEISNILSNDSGIYRCEVMHGIEDSQATVEVNVKGVVFHYRAISTRYTLDFENAKKACIQNSATIATPEQLQAAYDDGYHQCDAGWLSDQTVRYPIHNPREACYGDKDMFPGVRTYGVRETDETYDVYCYVEDMQGEVFYATSPEKFTFDEAEKQCKSLGARLATTGELYVAWKSGMDMCSAGWLGDRSVRYPISFARPNCGGNLVGVRTVYLHPTNQTGYPNPLSRYDAICYKAGPDEEITIQTITQPSLEIILPRNETEEEARGSIASLEPTDFTLTPTDFEEFFTATPELVLTEGQFENVTEEVFPLENITASLQTEAPGTADVTFSEGPEFNATTSLGEQIVPATAAPAEAGPEKDISKTGVVFHYRAASSRYSLNFTEAKQACLDNNAIIASPAQLQAAYEAGFDQCDAGWISDQTVRYPIVTPRENCHGDKNGFPGVRNYGVVNPNAHFDVYCYIDKLHGEVFFATQPDQFTFQEAKEFCDGQNASLASTGQLYMAWRLGLDKCRAGWLSDGSVRYPVVTPRRACGGDVPGVRTVYIHPNQTGFPDPLSKYHAFCFREEIPGFPSIEEELIVPEVVPEVEKLPSGEGPTSEIETVTEFENQTDMWLANVTVIPTIDSSVTVSPSVVPIVSPDPEDISGETSAIGSGVSGELSGLPSGVSSGDVSGADLSGQPSGAEESGFPSGTDISGVSGLPSGDISGDISGEPDISGLPSGFPSGVEDISGQGSGVPDISGLSSVPIETSGLPSGEPSGDEIISGSASGIEIISGLFSGTEDFSGIASGFPTITHVDTTWEEAVTKPTETELEGKGEIELSASGIELSGDLSGLEPSGEVSGFDLSGVTSSVVESSGDLSGIISSGDISGEPSGIIDLSGEVSGVTDISGDLSGLKDFSGETSGILDFSGELSGLSGESSGIISVSGGFSGDIDFSGEISGVREISGEISGVREISGEISGVREISGELSGVPDISGSASGILEIPSGVFLIDQALTELSQPSTKEEEGKGSVGISGFYSGDVDSSGDISGILDVSGGPSGFIDISGFVSGAVPSGDIDISGVSDISGATSGVEYVSGLQSGFDDLSGVPTVSHIDFTLKEPPTQGPDTQEAGEGPSVILEISGISGESLPSGTHEGSLESSGIYTSGFSQVTLVTDDTVEAITKPAVSQELGGVTFAYPIETSASGDISTEASATHETSGKQETSLEGSTEPSSGQLTFLPPEISETSESEITHSPEEAQQETELVTSVELGEVTDEPTIEAASPSSLIATESEGIIGSCADQPCGAGTCTEKEGRIYCACLPGYGGELCNIDIDECHSSPCVNGATCVDGIDSFKCLCLPSYGGDLCQIDLEVCEDGWTKFQGHCYKHFSERETWVDAENVCRYHQSHLASILTPEEQEFVNGNAQDYQWIGLNDKTIENDFRWSDANPLQYENWRPNQPDNFFTTGEDCVVMIWHERGEWNDVPCNYHLPFTCKKGTVACGDPPAVQNAYTLGKKKARYEISSLVRYQCNQGYLQRHVPIIRCLPTGHWDEPRIQCIDLTTYRRHKRNPRSRSRAIATSEQGKAH

Target ORF information:

RefSeq Version	XM_018092666.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis aggrecan (acan), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092666.1

ORF Insert Sequence:

ATGACCACTT TACTATTAGT GTTCGTTTGC TTGCGGGTCA TCGCTGCAGC GATATCCGTA 
GAAGTTTCAG ACCATCACAA CGCACTGAGT GTGATTATAC CTGTAGAAGC TCCACTGAAA 
GTCCAGCTTG GACATTCCCT TACCATCCCT TGTTTCTTCA TAAATGCTTT GGAAGATGTG 
ACTGTGGCTC CCTCAACAGC ACCCTTAACA CCAAGGATCA AGTGGAGCAA ACATGTCAAA 
GGAAAGGAAA CCGTCCTGCT GGTAGCCACA GATGGACATG TTCGTGTAAA TGACATCTAC 
CAAGGCCGTG TCTCTATGCC CACCTACCCA CTTGCCCCTA CAGATGTCAG CCTGGAAATT 
TCCAACATAC TGTCTAATGA TTCAGGAATC TACCGCTGTG AAGTGATGCA TGGGATAGAA 
GACAGCCAAG CTACAGTAGA GGTTAATGTG AAAGGTGTCG TTTTCCACTA CAGAGCAATC 
TCGACCAGAT ATACTTTGGA CTTTGAAAAT GCCAAAAAAG CCTGCATACA AAACAGTGCC 
ACCATTGCCA CCCCCGAGCA GCTCCAGGCA GCATATGATG ATGGTTACCA CCAATGTGAT 
GCTGGGTGGC TTTCCGACCA GACCGTTCGT TATCCAATTC ATAATCCACG TGAAGCCTGC 
TACGGAGACA AGGACATGTT TCCAGGAGTG AGAACATATG GAGTTCGCGA AACAGATGAA 
ACATATGATG TGTATTGCTA TGTTGAGGAT ATGCAGGGTG AAGTGTTTTA TGCAACATCT 
CCTGAAAAGT TCACCTTTGA TGAAGCAGAA AAGCAATGTA AGAGCCTTGG TGCCAGATTG 
GCCACAACTG GAGAGCTCTA TGTGGCATGG AAAAGTGGAA TGGATATGTG CAGTGCGGGT 
TGGTTGGGAG ACCGCAGTGT TAGGTACCCC ATATCCTTTG CTCGGCCAAA CTGTGGTGGA 
AATTTAGTAG GCGTAAGAAC AGTTTATCTC CACCCCACCA ACCAGACTGG ATATCCTAAT 
CCACTATCTC GGTATGATGC TATCTGCTAC AAAGCTGGAC CAGATGAGGA AATCACTATT 
CAAACCATTA CACAGCCAAG TTTAGAGATC ATCCTTCCCC GTAATGAGAC AGAAGAGGAG 
GCTCGTGGCA GTATTGCTTC TCTTGAACCA ACAGACTTTA CCCTTACACC TACTGATTTT 
GAGGAATTTT TCACAGCAAC CCCTGAGCTT GTGCTAACAG AAGGGCAATT TGAAAATGTC 
ACAGAAGAAG TATTTCCCCT TGAAAATATT ACAGCATCAC TCCAAACAGA AGCCCCTGGT 
ACAGCAGATG TGACATTCTC TGAGGGTCCA GAGTTTAATG CAACAACATC TTTAGGAGAG 
CAAATAGTTC CTGCAACAGC TGCTCCTGCT GAAGCTGGGC CAGAAAAAGA CATTTCTAAG 
ACTGGTGTTG TCTTTCATTA CCGAGCTGCT TCCAGTCGAT ATTCCCTTAA CTTCACCGAG 
GCTAAACAGG CATGTCTGGA CAACAATGCT ATCATTGCAA GCCCTGCTCA GCTCCAGGCT 
GCCTATGAAG CTGGATTTGA TCAATGTGAT GCTGGATGGA TATCAGACCA AACTGTCAGA 
TACCCAATAG TCACTCCAAG AGAAAACTGT CATGGAGACA AGAATGGATT TCCTGGGGTT 
AGAAATTATG GAGTGGTAAA CCCAAATGCA CATTTTGATG TGTACTGCTA CATTGATAAA 
CTTCATGGAG AGGTGTTTTT TGCCACTCAA CCAGACCAGT TTACTTTCCA AGAAGCAAAA 
GAATTTTGTG ATGGACAAAA TGCCTCATTG GCCTCCACTG GACAGCTCTA TATGGCCTGG 
AGGTTGGGGT TGGACAAGTG TAGAGCAGGC TGGTTGTCAG ATGGAAGTGT CCGCTACCCC 
GTTGTGACAC CTCGCAGAGC CTGTGGAGGA GACGTACCTG GAGTGCGGAC AGTCTACATT 
CATCCTAATC AAACAGGATT CCCTGATCCT CTGTCAAAAT ACCACGCCTT TTGTTTCCGA 
GAAGAGATTC CAGGTTTTCC ATCAATTGAG GAGGAGCTTA TTGTGCCTGA GGTAGTCCCA 
GAAGTAGAGA AATTACCTTC TGGAGAAGGA CCAACTTCCG AAATTGAGAC TGTGACTGAA 
TTTGAAAATC AGACAGATAT GTGGCTGGCA AATGTCACTG TAATTCCTAC TATTGATTCA 
TCAGTAACAG TCAGTCCTTC AGTTGTTCCA ATTGTGAGCC CAGATCCAGA AGATATAAGT 
GGAGAGACAT CAGCAATAGG CAGTGGAGTA AGTGGAGAAC TATCTGGATT GCCATCTGGA 
GTATCTAGTG GAGATGTGTC TGGTGCTGAT CTAAGTGGAC AACCATCAGG AGCTGAAGAA 
AGTGGATTTC CATCTGGCAC TGACATTTCT GGAGTGAGTG GACTGCCATC TGGTGACATA 
TCTGGTGACA TATCAGGTGA ACCTGATATT AGTGGACTGC CATCTGGATT TCCTTCAGGA 
GTTGAAGACA TATCAGGTCA AGGTTCTGGA GTTCCAGATA TAAGTGGGTT GTCTTCTGTA 
CCAATTGAAA CAAGTGGTCT CCCTTCTGGA GAGCCATCTG GAGATGAGAT AATTAGTGGT 
TCAGCTTCTG GAATTGAGAT AATTAGTGGC CTTTTTTCTG GTACAGAAGA TTTCAGTGGC 
ATTGCCTCAG GCTTCCCAAC AATTACACAT GTAGATACAA CTTGGGAAGA AGCTGTTACA 
AAACCAACAG AAACTGAACT AGAAGGAAAG GGAGAAATTG AATTAAGTGC ATCAGGAATA 
GAATTAAGTG GAGATCTTTC TGGCTTAGAG CCCAGTGGAG AGGTCTCTGG ATTTGACCTT 
AGTGGGGTTA CTTCATCAGT AGTAGAAAGC AGTGGAGATC TCTCTGGAAT TATTTCTAGT 
GGTGATATTA GTGGAGAGCC ATCAGGTATA ATTGATTTGA GTGGTGAAGT GTCAGGAGTA 
ACTGATATTA GTGGAGATTT GTCAGGATTA AAGGATTTTA GTGGTGAGAC ATCAGGAATA 
TTGGATTTTA GTGGTGAGCT ATCTGGCCTA AGTGGAGAAT CATCAGGAAT TATTTCTGTA 
AGTGGTGGAT TTTCTGGAGA TATAGATTTT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGA TTTCTGGAGT AAGAGAGATT AGTGGGGAGA TTTCTGGAGT AAGAGAGATT 
AGTGGGGAGC TTTCTGGAGT TCCTGACATC AGTGGCTCGG CCTCCGGTAT CTTAGAGATA 
CCATCAGGTG TTTTTCTTAT TGACCAAGCT TTGACAGAAC TCTCTCAGCC ATCAACAAAA 
GAAGAGGAAG GAAAAGGGTC TGTTGGAATA AGTGGCTTTT ATTCAGGAGA TGTTGACAGC 
TCTGGAGACA TATCTGGCAT CTTGGATGTC AGTGGTGGAC CTTCAGGTTT TATAGACATT 
AGTGGTTTTG TTTCAGGGGC TGTTCCTAGT GGTGATATAG ATATTAGTGG TGTATCAGAC 
ATAAGTGGTG CAACATCTGG AGTTGAATAT GTTAGTGGCT TACAATCTGG ATTTGATGAT 
CTAAGTGGAG TCCCTACTGT TTCTCATATT GATTTCACAT TAAAAGAGCC ACCAACTCAA 
GGTCCTGATA CTCAAGAAGC AGGAGAAGGT CCATCAGTAA TACTTGAGAT CAGTGGAATA 
TCTGGAGAAT CTTTGCCATC AGGAACTCAT GAAGGCAGTC TAGAAAGTTC AGGCATATAT 
ACATCAGGAT TTTCTCAAGT CACACTTGTT ACTGATGATA CTGTAGAAGC CATCACAAAA 
CCAGCAGTTT CACAAGAGCT AGGTGGTGTT ACTTTTGCAT ACCCAATAGA AACATCAGCA 
TCTGGTGATA TTAGCACCGA GGCATCTGCA ACTCATGAAA CCAGTGGAAA ACAAGAAACA 
TCTCTAGAAG GAAGCACTGA ACCAAGCTCT GGACAGCTGA CATTTTTGCC ACCAGAAATA 
TCTGAAACAT CAGAATCTGA AATAACTCAT TCTCCTGAGG AAGCACAACA GGAAACAGAA 
TTAGTTACTT CAGTTGAACT GGGTGAAGTA ACAGATGAAC CCACGATTGA AGCAGCCAGT 
CCTTCAAGTC TAATTGCAAC TGAGTCTGAA GGAATAATTG GATCATGTGC TGATCAACCC 
TGCGGAGCTG GAACATGTAC AGAAAAGGAA GGAAGAATAT ACTGTGCCTG CCTGCCAGGC 
TATGGTGGAG AACTTTGTAA TATAGACATT GATGAGTGCC ACTCAAGCCC CTGTGTGAAT 
GGAGCTACCT GCGTTGATGG CATCGACTCA TTCAAATGCT TATGCCTTCC CAGCTACGGA 
GGGGACCTAT GTCAGATCGA TCTAGAGGTG TGTGAGGATG GATGGACCAA GTTCCAGGGT 
CACTGCTACA AACACTTTTC AGAGAGAGAA ACTTGGGTGG ATGCAGAAAA CGTCTGTCGG 
TATCACCAGT CCCATCTAGC GAGCATTCTC ACACCTGAAG AGCAAGAATT TGTGAATGGT 
AACGCTCAGG ACTATCAATG GATTGGGCTG AATGATAAGA CAATTGAAAA TGATTTCCGC 
TGGTCAGATG CAAACCCCTT GCAATATGAA AACTGGAGGC CAAACCAACC AGATAACTTC 
TTCACCACGG GAGAAGATTG TGTTGTTATG ATCTGGCATG AACGTGGAGA ATGGAATGAT 
GTTCCCTGTA ACTACCACCT GCCATTTACA TGCAAAAAGG GAACAGTGGC TTGTGGTGAT 
CCTCCAGCAG TGCAAAATGC CTACACACTA GGAAAAAAGA AAGCACGTTA TGAAATCAGC 
TCCTTAGTGC GGTATCAATG TAACCAAGGC TACCTACAAC GTCATGTTCC TATAATCCGA 
TGTCTACCAA CTGGGCACTG GGATGAGCCC AGGATACAGT GCATAGATCT TACCACTTAC 
AGACGACACA AGAGAAACCC CAGAAGTCGG TCGAGAGCAA TTGCCACATC TGAACAGGGA 
AAGGCCCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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acan cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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